共查询到20条相似文献,搜索用时 31 毫秒
1.
Zecchin KG Seidinger AL Chiaratti MR Degasperi GR Meirelles FV Castilho RF Vercesi AE 《Journal of bioenergetics and biomembranes》2007,39(2):186-194
It has been previously shown that Walker 256 tumor cells express a high content of the anti-apoptotic protein Bcl-2 which
protects mitochondria against the damaging effects of Ca2+. In the present study, we analyze H2O2-induced apoptotic death in two different types of tumor cells: Walker 256 and SCC-25. Treatment with H2O2 (4mM) increased reactive oxygen species generation and the concentration of cytosolic free Ca2+. These alterations preceded apoptosis in both cell lines. In Walker cells, which show a high Bcl-2/Bax ratio, apoptosis was
dependent on calcineurin activation and independent of changes in mitochondrial membrane potential (Δ < eqid1 > m), as well as cytochrome c release. In contrast, in SCC-25 cells, which show a lower Bcl-2/Bax ratio, apoptosis was preceded by a decrease in Δ < eqid2 > m, mitochondrial permeability transition, and cytochrome c release. Caspase-3 activation occurred in both cell lines. The data suggest that although the high Bcl-2/Bax ratio protected
the mitochondria of Walker cells from oxidative stress, it was not sufficient to prevent apoptosis through calcineurin pathways. 相似文献
2.
It is believed that ROS-induced oxidative stress triggers numerous signaling pathways which are involved in neurodegenerative
diseases, including Alzheimer’s disease. To find the effective drugs for neurodegenerative diseases, the deep delve into molecular
mechanisms underlie these diseases is necessary. In the current study, we investigated the effects of flavonoid baicalein
on H2O2-induced oxidative stress and cell death in SK-N-MC cells. Our results revealed that the treatment of SK-N-MC cells with H2O2 led to a decrease in cell viability through phosphorylation and activation of extracellular signal-regulated kinases (ERKs)
and c-Jun N-terminal kinases (JNKs) pathways followed by increase in Bax/Bcl2 ratio and initiation of caspase-dependent apoptotic
pathways. In addition, our results showed that the exposure of SK-N-MC cells to H2O2 ended up in reduction of glutathione (GSH) levels of SK-N-MC cells via JNK/ERK-mediated down-regulation of γ-glutamyl-cysteine
synthetase (γ-GCS) expression. Our results demonstrated that flavonoid baicalein protected against H2O2-induced cell death by inhibition of JNK/ERK pathways activation and other key molecules in apoptotic pathways, including
blockage of Bax and caspase-9 activation, induction of Bcl-2 expression and prevention of cell death. Baicalein supported
intracellular defense mechanisms through maintaining GSH levels in SK-N-MC cells by the removal of inhibition effects of JNK/ERK
pathways from γ-GCS expression. In addition, baicalein attenuated lipid and protein peroxidation and intracellular reactive
oxygen species in SK-N-MC cells. In accordance with these observations, baicalein can be a promising candidate in antioxidant
therapy and designing of natural-based drug for ROS-induced neurodegenerative disorders. 相似文献
3.
Cinnamomum japonicum Sieb. is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value. In this study,
we successfully developed a large-scale micropropagation protocol for C. japonicum for the first time. Sterilized shoots were excised and used as explants for
shoot induction on several basal media, supplemented with different concentrations
of plant growth regulators (PGRs), such as Thidiazuron (TDZ), N6
-Benzyladenine
(6-benzylaminopurine) (BA), α-naphthaleneacetic acid (NAA) and Gibberellic acid
(GA3). After comparison, the most efficient medium for shoot regeneration
was 1/2 Murashige and Skoog (MS) medium containing 0.5 mg L–1 BA,
0.05 mg L–1 NAA and 0.2 mg L–1 GA3, which resulted in an average number of
induced shoots per explant and shoot length of 5.2 and 1.62 cm at 28 d, respectively. Then, elongated adventitious shoots were transferred to induce roots.
86.7% of shoots was able to root on 1/2 MS medium supplemented with 0.5 mg
L–1 NAA and 0.1 mg L–1 BA. The earliest rooting time observed was after 21 d
and the average root length was up to 3.3 cm after 28 d. Our study shows that
C. japonicum can be successfully regenerated through de novo organogenesis,
which lays a foundation for future transformation research on this tree. 相似文献
4.
Apoptosis modulatory activities of transiently expressed Bcl-2: Roles in cytochrome c release and Bax regulation 总被引:12,自引:0,他引:12
Hou Q Cymbalyuk E Hsu SC Xu M Hsu YT 《Apoptosis : an international journal on programmed cell death》2003,8(6):617-629
Bcl-2 and Bcl-XL are pro-survival members of the Bcl-2 family. These proteins have been shown to antagonize the pro-apoptotic activity of Bax and promote cell survival through blocking Bax translocation from the cytosol to mitochondria and by preventing the release of cytochrome c. However, it has been recently reported that transiently expressed Bcl-2 unexpectedly leads to significant cell toxicity. To study this intriguing phenomenon, we have carried out further analyses into the properties of transiently expressed Bcl-2. We found that various isoforms of human and different species of Bcl-2 were equally capable of inducing apoptosis. In addition, we discovered that transient expression of Bcl-2, unlike its pro-survival homolog Bcl-XL, can lead to the release of cytochrome c from mitochondria and that the resulting cell death can be inhibited by caspase and calpain inhibitors. Moreover, we have shown that unlike the pro-apoptotic protein Bid, the toxicity associated with the transient expression of Bcl-2 occurs independent of the activity of the endogenous Bax. Finally, we found that in spite of its intrinsic toxicity, transiently expressed Bcl-2 is fully capable of blocking the ectopically expressed Bax from localizing to mitochondria. Taken together, these studies demonstrate that transiently expressed Bcl-2 displays opposing functional properties. 相似文献
5.
Jafari Anarkooli I Sankian M Vahedi F Bonakdaran S Varasteh AR Haghir H 《Cellular and molecular neurobiology》2009,29(1):133-140
Aims Effects of insulin and ascorbic acid on expression of Bcl-2 family proteins and caspase-3 activity in hippocampus of diabetic
rats were evaluated in this study. Methods Diabetes was induced in Wistar male rats by streptozotocin (STZ). Six weeks after verification of diabetes, the animals were
treated for 2 weeks with insulin or/and ascorbic acid in separate groups. Hippocampi of rats were removed and evaluation of
Bcl-2, Bcl-xL, and Bax proteins expression in frozen hippocampi tissues were done by SDS-PAGE electrophoresis and blotting. The Bcl-2,
Bcl-xL, and Bax proteins bands were visualized after incubation with specific antibodies using enhanced chemiluminescences method.
Caspase-3 activity was determined using the caspase-3/CPP32 Fluorometric Assay Kit. Results Diabetic rats showed increase in Bax protein expression and decrease in Bcl-2 and Bcl-xL proteins expression. The Bax/Bcl-2 and Bax/Bcl-xL ratios were found higher compared with non-diabetic control group. Treatments with insulin and/or ascorbic acid were resulted
in decrease in Bax protein expression and increase in Bcl-2 and Bcl-xL proteins expression. The Bcl-2/Bax and Bcl-xL/Bax ratios were found higher in treated groups than untreated diabetic group. Caspase-3 activity level was found higher in
diabetic group compared with non-diabetic group. Treatment with insulin and ascorbic acid did downregulated caspase-3 activity.
Conclusions Our data provide supportive evidence to demonstrate the antiapoptotic effects of insulin and ascorbic acid on hippocampus
of STZ-induced diabetic rats. 相似文献
6.
《Molecular & cellular biomechanics : MCB》2018,15(1):21-36
Dendritic cells (DCs) are the most potent professional antigen presenting cells as now known, which play critical roles in the initiation, programming and regulation of the immune response. Transforming growth factor-β1 (TGF-β1), one of the major suppressive cytokines in tumor microenvironment, can deteriorate the biomechanical characteristics and motility of mature dendritic cells (mDCs),but the underlying molecular mechanisms are not well defined. In this study, the effects of TGF-β1 on the motilities and T cell priming capabilities of mDCs as well as the molecular regulatory mechanisms were investigated. The results showed that the cytoskeleton (F-actin) organizations of mDCs were abnormally remodeled by TGF-β1. Simultaneously, the migration and immune priming capabilities of mDCs were impaired by TGF-β1 via Smad2/3 signaling pathway. It’s significant for further understanding the interaction of DCs and TGF-β1 in tumor host, as well as the immune escape mechanism of cancer, which may be important for enhancing the clinical efficiency of DCs-based immunotherapy against cancer. 相似文献
7.
Chen SP Wu JL Su YC Hong JR 《Apoptosis : an international journal on programmed cell death》2007,12(6):1043-1060
Nervous necrosis virus (NNV)-induced, host cell apoptosis mediates secondary necrosis by an ill-understood process. In this
study, redspotted grouper nervous necrosis virus (RGNNV) is shown to induce mitochondria-mediated necrotic cell death in GL-av
cells (fish cells) via cytochrome c release, and anti-apoptotic proteins are shown to protect these cells from death. Western blots revealed that cytochrome
c release coincided with disruption of mitochondrial ultrastructure and preceded necrosis, but did not correlate with caspases
activation. To identify the mediator(s) of this necrotic process, a protein synthesis inhibitor (cycloheximide; CHX; 0.33 μg/ml)
was used to block cytochrome c release as well as PS exposure and mitochondrial membrane permeability transition pore (MMP) loss. CHX (0.33 μg/ml) completely
blocked viral protein B2 expression, and partly blocked protein A, protein α, and a pro-apoptotic death protein (Bad) expression.
Overexpression of B2 gene increased necrotic-like cell death up to 30% at 48 h post-transfection, suggesting that newly synthesized protein (B2)
may be involved in this necrotic process. Finally, necrotic death was prevented by overexpression of Bcl-2 family proteins,
zfBcl-xL and xfMcl-1a. Thus, new protein synthesis and release of cytochrome c are required for RGNNV-induced necrotic cell death, which can be blocked by anti-apoptotic Bcl-2 members.
J.-L. Wu and J.-R. Hong contributed equally to the research. 相似文献
8.
Eliana Arias-Pérez Carlos Alberto Lecona-Guzmán Federico Antonio Gutiérrez-Miceli Joaquín Adolfo Montes-Molina Nancy Ruiz-Lau 《Phyton》2021,90(6):1741-1748
The present study aimed to develop a protocol for somatic embryogenesis and encapsulation of coffee embryos (Coffea arabica L.), for the conservation of genotypes with characteristics of commercial interest. Somatic embryos were induced from leaf explants in Murashige and Skoog medium (MS) supplemented with 1 mg · L−1 of 2,4-dichlorophenoxiacetic acid (2,4-D) combined with 2 mg · L−1 of benzyladenine (BA). Somatic embryos (SE) at the globular stage were encapsulated in a sodium alginate matrix; two treatments were tested: MS + 5 mg · L−1 BA + 1 mg · L−1 NAA + 3% (w/v) alginate, and MS + 7 mg · L−1 BA + 5.7 mg · L−1 indoleacetic acid (IAA) + 3% (w/v) alginate. Alginate was complexed with 100 mM calcium chloride (CaCl2). Viability of the encapsulated SE was determined by staining with 0.01% fluorescein diacetate (FDA) after 0, 15, 30, and 45 days of storage at 4°C. Embryo viability was 100% in both treatments. 相似文献
9.
Increases in Bcl-2 and cleavage of caspase-1 and caspase-3 in human brain after head injury. 总被引:17,自引:0,他引:17
R S Clark P M Kochanek M Chen S C Watkins D W Marion J Chen R L Hamilton J E Loeffert S H Graham 《FASEB journal》1999,13(8):813-821
The bcl-2 and caspase families are important regulators of programmed cell death in experimental models of ischemic, excitotoxic, and traumatic brain injury. The Bcl-2 family members Bcl-2 and Bcl-xL suppress programmed cell death, whereas Bax promotes programmed cell death. Activated caspase-1 (interleukin-1beta converting enzyme) and caspase-3 (Yama/Apopain/Cpp32) cleave proteins that are important in maintaining cytoskeletal integrity and DNA repair, and activate deoxyribonucleases, producing cell death with morphological features of apoptosis. To address the question of whether these Bcl-2 and caspase family members participate in the process of delayed neuronal death in humans, we examined brain tissue samples removed from adult patients during surgical decompression for intracranial hypertension in the acute phase after traumatic brain injury (n=8) and compared these samples to brain tissue obtained at autopsy from non-trauma patients (n=6). An increase in Bcl-2 but not Bcl-xL or Bax, cleavage of caspase-1, up-regulation and cleavage of caspase-3, and evidence for DNA fragmentation with both apoptotic and necrotic morphologies were found in tissue from traumatic brain injury patients compared with controls. These findings are the first to demonstrate that programmed cell death occurs in human brain after acute injury, and identify potential pharmacological and molecular targets for the treatment of human head injury. 相似文献
10.
Abdelmajid Krouma 《Phyton》2023,92(7):2133-2150
Iron is an essential element for plants as well as all living organisms, functioning in various physiological and
biochemical processes such as photosynthesis, respiration, DNA synthesis, and N2 fixation. In the soil, Fe bioavailability is extremely low, especially under aerobic conditions and at high pH ranges. In contrast, plants with
nodules on their roots that fix atmospheric nitrogen need much more iron. To highlight the physiological traits
underlying the tolerance of N2-fixing common bean to iron deficiency, two genotypes were hydroponically cultivated in a greenhouse: Coco nain (CN) and Coco blanc (CB). Plants were inoculated with an efficient strain of
Rhizobium tropici, CIAT899, and received a nutrient solution added with 0 µM Fe (severe Fe deficiency, SFeD),
5 µM Fe (moderate Fe deficiency, MFeD) or 45 µM Fe (control, C). Several physiological parameters related to
photosynthesis and symbiotic nitrogen fixation were then analyzed. Iron deficiency significantly reduced whole
plant and nodule growth, chlorophyll biosynthesis, photosynthesis, leghemoglobin (LgHb), nitrogenase (N2ase)
activity, nitrogen, and Fe nutrition, with some genotypic differences. As compared to CB, CN maintained better
Fe allocation to shoots and nodules, allowing it to preserve the integrity of its photosynthetic and symbiotic apparatus, thus maintaining the key functional traits of the plant metabolism (chlorophyll biosynthesis and photosynthesis in shoots, leghemoglobin accumulation, and nitrogenase activity in root nodules). Plant growth
depends on photosynthesis, which needs to be supplied with sufficient iron and nitrogen. Fe deficiency stress
index (FeD-SI) and Fe use efficiency (FeUE) are two physiological traits of tolerance that discriminated the
studied genotypes. 相似文献
11.
Chinese hickory (Carya cathayensis Sarg.) is a popular nut tree in China, but there is little information about the
influences of fertilization on soil CO2 efflux and soil microbial biomass. This study evaluated the short-term
effects of different fertilizer applications on soil CO2 efflux and soil microbial biomass in Chinese hickory stands.
Four fertilizer treatments were established: control (CK, no fertilizer), inorganic fertilizer (IF), organic fertilizer
(OF), and equal parts organic and inorganic N fertilizers (OIF). A field experiment was conducted to measure
soil CO2 effluxes using closed chamber and gas chromatography techniques. Regardless of the fertilization practices, soil CO2 effluxes of all the treatments showed a similar temporal pattern, with the highest value in summer
and the lowest in winter. The mean annual soil CO2 efflux in the IF treatment was significantly higher than that in
the CK, OIF, and OF treatments. There was no significant difference in soil CO2 efflux between the OIF, OF, and
CK treatments. Soil CO2 effluxes were significantly affected by soil temperature. Soil dissolved organic carbon
(DOC) was positively correlated with soil CO2 efflux only in the CK treatment. Regression analysis, including soil
temperature, moisture, and DOC, showed that soil temperature was the primary factor influencing soil CO2
effluxes. Both OF and OIF treatments increased concentrations of soil microbial biomass carbon (MBC) and
microbial biomass nitrogen (MBN), but decreased the ratio of MBC:MBN. These results reveal that applying
organic fertilizer, either alone or combined with inorganic fertilizer, may be the optimal strategy for mitigating
soil CO2 emission and improving soil quality in Chinese hickory stands. 相似文献
12.
Aconitum vilmorinianum, a well-known traditional Chinese herb, is recently being threatened by overexploitation
and environment disturbance. This study was conducted to provide propagation methods through in vitro germination and explant cultivation. Germination was stimulated up to 66.00% on Murashige and Skoog (MS) medium containing 2.0 mg L−1 6-benzylaminopurine (BAP), 0.1 mg L−1 1-napthaleneacetic acid (NAA), and 30 g L−1
sucrose. Three bacteria (Pantoea agglomerans, Erwinia persicina, and Pseudomonas tolaasii) would be responsible
for consistent contamination during germination. The latter two were effectively eradicated after disinfected. The
influence of explant types and hormone combinations on direct and indirect organogenesis was evaluated in the
present work. The frequency of shoot induction from axillary bud explants was 100% on the MS fortified with
2.0 mg L−1 BAP and 0.3 mg L−1 NAA. Shoots multiplication was optimized on MS medium supplemented with
0.1 mg L−1 thidiazuron (TDZ) and 0.1 mg L−1 NAA. High callus induction percentage (96.67%) was obtained
from stem segments on MS medium with 2.0 mg L−1 2,4-D, then successfully regenerated into shoots on MS medium in the presence of 0.1 mg L−1 TDZ and 0.2 mg L−1 NAA. The present work could be useful for the utilization
and conservation of this valuable species. 相似文献
13.
Fatemeh Shaerzadeh Shabnam Zeighamy Alamdary Mohammad Ali Esmaeili Nazanin Namazi Sarvestani Fariba Khodagholi 《Neurochemical research》2011,36(12):2216-2226
Herein, we investigated the protective effect of Salvia sahendica against H2O2-induced cell death in rat pheochromocytoma (PC12) cells. Our data show that S. sahendica blocks apoptosis pathway by inhibition of cytochrome c release from mitochondria and leakage of calcium from endoplasmic reticulum. It also activates/inactivates two members of
Bcl-2 family, Bax and Bcl-2. Bax inhibition and Bcl-2 activation suppress release of cytochrome c from mitochondria that prevents cleavage of caspase-3. Besides S. sahendica suppresses ER stress via attenuation of intracellular levels of calcium. Suppression of ER stress decreased calpain activation
and subsequently cleavage of caspase-12. Altogether, these results indicate that S. sahendica protects PC12 cells treated with H2O2 via suppression of upstream factors of apoptosis pathway. While oxidative stress is an early event in Alzheimer disease,
it seems that S. sahendica prevents deleterious effects of reactive oxygen species by stabilizing mitochondrial membranes and inhibiting ER stress. 相似文献
14.
Abdulkhaliq Alshoaibi 《Phyton》2022,91(1):45-55
Pearl millet (Pennisetum spicatum (L.) Körn.) and maize (Zea mays L.) are C4 grass species grown for feeding humans and animals in Almadinah Almunawwarah, which is in the western part of Saudi Arabia. During the winter, the mean temperature, which drops to 14°C, represents a major problem for the growth of these species in this region. Therefore, the objectives of this research were to investigate the growth response and the photosynthetic performance of P. spicatum and Z. mays under a low temperature stress. The treatments involved daytime and nighttime temperatures of 14/12°C (low temperature) and 24/22°C (optimum temperature). The results indicated that low temperature significantly reduced all growth and physiological parameters, including seed germination, leaf expansion, leaf area, shoot length and root length of the two species compared to those of the control. Additionally, the low temperature significantly decreased the light-saturated assimilation rate (Asat), quantum yield (ϕ), saturated rate of carbon dioxide uptake (Amax) and efficiency of carboxylation on both species compared to those of the control. Moreover, the values of Fv/Fm and the chlorophyll contents of both species were significantly reduced by low temperature compared to those of the control. It can be concluded that both species had little tolerance to low temperatures. 相似文献
15.
Zhenqiu Liu Laurence S Magder Terry Hyslop Li Mao 《Algorithms for molecular biology : AMB》2010,5(1):30
It has been demonstrated that genes in a cell do not act independently. They interact with one another to complete certain
biological processes or to implement certain molecular functions. How to incorporate biological pathways or functional groups
into the model and identify survival associated gene pathways is still a challenging problem. In this paper, we propose a
novel iterative gradient based method for survival analysis with group L
p
penalized global AUC summary maximization. Unlike LASSO, L
p
(p < 1) (with its special implementation entitled adaptive LASSO) is asymptotic unbiased and has oracle properties [1]. We first extend L
p
for individual gene identification to group L
p
penalty for pathway selection, and then develop a novel iterative gradient algorithm for penalized global AUC summary maximization
(IGGAUCS). This method incorporates the genetic pathways into global AUC summary maximization and identifies survival associated
pathways instead of individual genes. The tuning parameters are determined using 10-fold cross validation with training data
only. The prediction performance is evaluated using test data. We apply the proposed method to survival outcome analysis with
gene expression profile and identify multiple pathways simultaneously. Experimental results with simulation and gene expression
data demonstrate that the proposed procedures can be used for identifying important biological pathways that are related to
survival phenotype and for building a parsimonious model for predicting the survival times. 相似文献
16.
17.
In this study, the effect of aucubin on H2O2-induced apoptosis was studied by using a rat pheochromocytoma (PC12) cell line. We have analyzed the apoptosis of H2O2-induced PC12 cells, H2O2-induced apoptosis appeared to correlate with lower Bcl-2 expression, higher Bax expression and sequential activation of caspase-3
leading to cleavage of poly-ADP-ribose polymerase (PARP). Aucubin not only inhibited lower Bcl-2 expression, high Bax expression,
but also modulated caspase-3 activation, PARP cleavage, and eventually protected against H2O2-induced apoptosis. These results indicated that aucubin can obstruct H2O2-induced apoptosis by regulating of the expression of Bcl-2 and Bax, as well as suppression of caspases cascade activation. 相似文献
18.
P. M. Abdul-Muneer Mathew Long Adriano Andrea Conte Vijayalakshmi Santhakumar Bryan J. Pfister 《Molecular neurobiology》2017,54(6):3964-3975
We investigated the hypothesis that high Ca2+ influx during traumatic brain injury induces the activation of the caspase-1 enzyme, which triggers neuroinflammation and cell apoptosis in a cell culture model of neuronal stretch injury and an in vivo model of fluid percussion injury (FPI). We first established that stretch injury causes a rapid increase in the intracellular Ca2+ level, which activates interleukin-converting enzyme caspase-1. The increase in the intracellular Ca2+ level and subsequent caspase-1 activation culminates into neuroinflammation via the maturation of IL-1β. Further, we analyzed caspase-1-mediated apoptosis by TUNEL staining and PARP western blotting. The voltage-gated sodium channel blocker, tetrodotoxin, mitigated the stretch injury-induced neuroinflammation and subsequent apoptosis by blocking Ca2+ influx during the injury. The effect of tetrodotoxin was similar to the caspase-1 inhibitor, zYVAD-fmk, in neuronal culture. To validate the in vitro results, we demonstrated an increase in caspase-1 activity, neuroinflammation and neurodegeneration in fluid percussion-injured animals. Our data suggest that neuronal injury/traumatic brain injury (TBI) can induce a high influx of Ca2+ to the cells that cause neuroinflammation and cell death by activating caspase-1, IL-1β, and intrinsic apoptotic pathways. We conclude that excess IL-1β production and cell death may contribute to neuronal dysfunction and cognitive impairment associated with TBI. 相似文献
19.
Wuzhong Yin Hongxia Yang Yantong Wang Ping Feng Yao Deng Yang Liu Danyang Chen Yijie Ban Weichi Liu Guanghua He Nan Wang 《Phyton》2022,91(4):727-744
The mitogen-activated protein kinase (MAPK) cascade is important in stress signal transduction and plant development. In the present study, we identified a rice (Oryza sativa L.) mutant with reduced fertility, Oryza sativa
mitogen-activated protein kinase 6 (osmapk6), which harbored a mutated MAPK gene. Scanning and transmission
electron microscopy, quantitative RT-PCR analysis, TUNEL assays, RNA in situ hybridization, longitudinal and
transverse histological sectioning, and map-based cloning were performed to characterize the osmapk6 mutant.
The gene OsMAPK6 was expressed throughout the plant but predominantly in the microspore mother cells, tapetal cells, and microspores in the anther sac. Compared with the wild type, the total number of microspores was
reduced in the osmapk6 mutant. The formation of microspore mother cells was reduced in the osmapk6 anther
sac at an early stage of anther development, which was the primary reason for the decrease in the total number of
microspores. Programmed cell death of some tapetal cells was delayed in osmapk6 anthers and affected exine formation in neighboring microspores. These results suggest that OsMAPK6 plays pivotal roles in microspore
mother cell formation and tapetal cell degradation. 相似文献
20.
Jutamart Monkai Ruvishika S. Jayawardena Sajeewa S. N. Maharachchikumbura Kevin D. Hyde 《Phyton》2020,89(3):765-777
We isolated an interesting fungus from dead leaves of Magnolia liliifera
collected from Chiang Mai, Thailand. The novel strain is related to Plectosphaerellaceae based on the morphology of its asexual morph and the analysis of
sequence data. Phylogenetic analyses using a combined gene analysis of LSU
and ITS sequence data showed that this strain is clustered in the same clade with
Furcasterigmium furcatum with high statistical support. The new strains produced
the asexual morph in culture which is morphologically similar to F. furcatum.
Thus, we identified this strain as the sexual morph of F. furcatum. This is the first
record of sexual morph for the monotypic genus Furcasterigmium and the first
record of this genus on Magnolia. 相似文献