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1.
Summary
D. melanogaster males of
bb/O genetic constitution undergoing rDNA magnification were mated singly to XXbb
+/O females, yielding
bb/O male progeny, and to XNO-w sn bb
+ fameles, yielding
bb/XNO- females. The male and female offspring were scored for the bb
+ phenotype.Results show that there is a higher percentage of bb
+ flies in the
bb/O male progeny than in
bb/XNO- females progeny, in single crosses as well as in the combined data. rRNA/DNA hybridization experiments agree with this observation, by showing that the rDNA content in the progeny of premagnified flies was higher in the sons than in the daughters.These data indicate that the increase of ribosomal RNA genes is not due to a stable event such as an unequal mitotic sister exchange, whereas they do not contrast with the extracopy model. 相似文献
2.
P. Pic 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1978,123(2):155-162
Summary In seawater (SW)-adaptedMugil andFundulus, gill effluxes of Na+
and of Cl–
and the simultaneously recorded transgill potential (P.D.) differ according to whether they are measured in stressed or rested animals.In rested animals of the two species, transfer to Ringer's solution considerably reduces the P.D. but not
. InFundulus,
is also decreased. Transfer of the two species from SW to fresh water (FW) reduces
and
by 75 to 85% and leads to a large inversion of P.D. When K+ is added to FW, a gill depolarization occurs, as well as a large increase of
and
.These results suggest that: 1) the P.D. originates primarily from the diffusion of cations, the gill permeability to Na+ (
) being greater than that to Cl– (
), 2) a Cl–/Cl– exchange independent of P.D. is associated with the Cl– pump; 3) Cl– pump activity is linked to Na+/K+ exchange which in turn is associated to a Na+/Na+ exchange diffusion mechanism.In stressed individuals of the two species, the P.D. in SW, as well as the P.D. changes observed during transfer experiments, are considerably reduced. The decrease of
and
observed after transfer from SW to FW are also minimised. Changes are smaller inFundulus. The decrease of P.D. characterizing stressed animals may be at least in part due to a 3 to 4 fold increase of
which becomes equal to
in both species.As a result of stress, the K+-activated Na+ and Cl– excretion mechanisms are totally inhibited inFundulus and partially so inMugil.Stress response seems more intense inFundulus and recovery from stress faster inMugil. 相似文献
3.
Philippe Pelupessy Elisabetta Chiarparin Ranajeet Ghose Geoffrey Bodenhausen 《Journal of biomolecular NMR》1999,14(3):277-280
A method is presented to determine both and backbone angles in proteins simultaneously. This is achieved by measuring the effect on two-spin coherences of cross-correlation between 15 N-1HN and 13
vectors. The cross-correlation rates are obtained by comparing two complementary three-dimensional experiments. 相似文献
4.
Kenth Dimberg Lars B. Höglund 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1987,157(4):405-412
Summary Carbonic anhydrase (CA) activities in gills and venous blood, acid-base balance, and haematological variables were studied during environmental hypercapnia in rainbow trout (Salmo gairdneri). Batches of 8–10 fish were exposed to about 3 or 13 mmHg
in flow-through tests of various duration from 4 h to 80 days.After initial acidosis, blood pH rose above pre-experimental values. At 3 mmHg it became normal again within 21 days, while at 13 mmHg the overshoot lasted for 80 days. In fish acclimated for 3 weeks or more to 13 mmHg
, blood HCO
3
–
increased four to five times while plasma Cl– levels were lower and K+ higher. Na+ levels did not show any consistent trend associated with exposure to hypercapnia. After an initial acidaemia, Hct, Hb, and RBC remained relatively constant.Patterns of change in CA activity differed between gills and erythrocytes. Initially, blood CA decreased at both
levels. It then began rising after about 3 weeks and tended to reach pre-experimental values by 80 day's hypercapnia. At 13 mmHg
, gill CA increased to twice the pre-experimental level. Compared with blood CA, gill CA appeared to be more specifically involved in fish acclimation to hypercapnia, which demands an increase in blood bicarbonate to provide a sufficient buffering capacity. Increased CA indicates that the gill enzyme may play a more important role than blood CA in acid-base regulation in fish during hypercapnia.Abbreviations
CA
carbonic anhydrase
-
Hb
haemoglobin
-
Hct
haematocrit value
-
RBC
red blood cells 相似文献
5.
Lemaster DM 《Journal of biomolecular NMR》1995,6(4):366-374
Summary The Lipari-Szabo dynamical formalism is extended by setting the time constants of the Lorentzian terms to
and
. This analysis is compared to the earlier proposed three-parameter
extended model free formalism with regard to the range of equivalence and the advantages of the simplified two-parameter (S
inff
sup2
,S
infH
sup2
) and (S
inff
sup2
,S
infN
sup2
) representations. Spectral density components are calculated and compared to those obtained from the spectral density analysis formalism. Protein relaxation data, commonly analyzed in terms of the two-parameter
representation, may correspond to a dynamically heterogeneous behaviour that is more appropriately represented in terms of a fast limit order parameter and a second, lower frequency order parameter. 相似文献
6.
The growth yield of Chlorella vulgaris, Y
kJ defined as g cells harvested per kJ of light energy absorbed by the cells, was assessed in a turbidostat culture by varying CO2 and O2 partial pressures (
and
). The value of Y
kJ ranged from 3.1×10-3 to 5.0×10-3 g cells/kJ under light-limited conditions [
= 1.02.4%,
= 065%; total pressure of gas (composed of CO2, O2 and N2)=1 atm]. In the light-limited environment, the algal specific growth rate deteriorated appreciably with the increase of
. The deterioration accounts for the above range of Y
kJ observed. The growth inhibition due to oxygen that was defined by subtracting from 1.0 the ratio of at given values of
to that at
= 0% extended from 0.07–0.30 (7–30%). However, glycolate could not be detected in the turbidostat culture. Isotopic experiments on the specific rate of 14CO2 uptake also revealed that the inhibition due to oxygen was from 22–38% when
was varied from 0 to nearly 100%. These effects of oxygen were discussed, referring to the activity of ribulose-1,5-bisphosphate carboxylase that is inhibited competitively by oxygen.Non-Standard Abbreviations INH
isonicotinic acid hydrazide
- PPO
2,5-diphenyloxazole
- DCMU
3-(-3,4-dichlorophenyl)-1,1-dimetylurea
- CA
carbonic anhydrase
- RuP2
ribulose-1,5-bisphosphate 相似文献
7.
Raif Musa-Aziz Lihong Jiang Li-Ming Chen Kevin L. Behar Walter F. Boron 《The Journal of membrane biology》2009,228(1):15-31
Others have shown that exposing oocytes to high levels of (10–20 mM) causes a paradoxical fall in intracellular pH (pHi), whereas low levels (e.g., 0.5 mM) cause little pHi change. Here we monitored pHi and extracellular surface pH (pHS) while exposing oocytes to 5 or 0.5 mM NH3/NH4
+. We confirm that 5 mM causes a paradoxical pHi fall (−ΔpHi ≅ 0.2), but also observe an abrupt pHS fall (−ΔpHS ≅ 0.2)—indicative of NH3 influx—followed by a slow decay. Reducing [NH3/NH4
+] to 0.5 mM minimizes pHi changes but maintains pHS changes at a reduced magnitude. Expressing AmtB (bacterial Rh homologue) exaggerates −ΔpHS at both levels. During removal of 0.5 or 5 mM NH3/NH4
+, failure of pHS to markedly overshoot bulk extracellular pH implies little NH3 efflux and, thus, little free cytosolic NH3/NH4
+. A new analysis of the effects of NH3 vs. NH4
+ fluxes on pHS and pHi indicates that (a) NH3 rather than NH4
+ fluxes dominate pHi and pHS changes and (b) oocytes dispose of most incoming NH3. NMR studies of oocytes exposed to 15N-labeled show no significant formation of glutamine but substantial accumulation in what is likely an acid intracellular compartment. In conclusion, parallel measurements of pHi and pHS demonstrate that NH3 flows across the plasma membrane and provide new insights into how a protein molecule in the plasma membrane—AmtB—enhances
the flux of a gas across a biological membrane.
相似文献
Walter F. Boron (Corresponding author)Email: |
8.
P. Badia A. Lorenzo T. Gomez A. Bolaños 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1988,157(6):865-871
Summary Ion transport processes in the ileum of the lizard,Gallotia (=Lacerta) galloti was examined in vitro by measuring Na22 and Cl36 fluxes across short-circuited preparations.In Ringer-bicarbonate solution there was both a net sodium flux (
) and a net chloride flux (
) from mucosa to serosa. The inequality between
and short-circuit current (I
sc) suggests that part of the net sodium transport is the result of an electrically neutral transport mechanism or that another electrogenic mechanism opposite in sign is contributing to the short-circuit current.In the absence of sodium, the short-circuit current and net chloride flux were abolished. In the absence of chloride, the net sodium was reduced but not abolished and the short-circuit current was unchanged.From an analysis of the effects of the inhibitors furosemide, amiloride, disulfonic stilbene (DIDS) and acetazolamide, a plausible model was developed to explain the characteristics of these transports. It is proposed that the entry of sodium into the cell across the luminal membrane occurs by two pathways. Part occurs by the antiport Na+H+ and part by an electrogenic pathway. The entry of chloride is by the antiport Cl–HCO
3
–
.Symbols and abbreviations
DIDS 4,4
diisothiocyanatostilbene-2,2-disulfonic acid
-
G
t
tissue conductance
-
I
sc
short circuit current
-
m
mucosal
-
PD
potential difference
-
s
serosal 相似文献
9.
B. Culik A. J. Woakes D. Adelung R. P. Wilson N. R. Coria H. J. Spairani 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1990,160(1):61-70
Summary The energy requirements of Adélie penguin (Pygoscelis adeliae) chicks were analysed with respect to body mass (W, 0.145–3.35 kg, n=36) and various forms of activity (lying, standing, minor activity, locomotion, walking on a treadmill). Direct respirometry was used to measure O2 consumption (
) and CO2 production. Heart rate (HR, bpm) was recorded from the ECG obtained by both externally attached electrodes and implantable HR-transmitters. The parameters measured were not affected by hand-rearing of the chicks or by implanting transmitters. HR measured in the laboratory and in the field were comparable. Oxygen uptake ranged from
in lying chicks to
at maximal activity, RQ=0.76. Metabolic rate in small wild chicks (0.14–0.38 kg) was not affected by time of day, nor was their feeding frequency in the colony (Dec 20–21). Regressions of HR on
were highly significant (p< 0.0001) in transmitter implanted chicks (n=4), and two relationships are proposed for the pooled data, one for minor activities (
), and one for walking (
). Oxygen consumption, mass of the chick (2–3 kg), and duration of walking (T, s) were related as
, whereas mass-specific O2 consumption was related to walking speed (S, m·s-1) as
.Abbreviations
bpm
beats per minute
-
D
distance walked (m)
-
ECG
electrocardiogram
-
HR
heart rate (bpm)
-
ns
number of steps
-
RQ
respiratory quotient
-
S
walking speed (m·s-1)
-
T
time walked (s)
-
W
body mass (kg) 相似文献
10.
Desulfovibrio vulgaris (strain Marburg) was grown on H2 and sulfate as sole energy source in a chemostat limited by the sulfate supply. The biomass concentration and the sulfate concentration in the culture were determined as a function of the dilution rate. From the data a K
S (saturation constant) for sulfate of 10 M, a max of 0.23 h–1, and a
of 13 g/mol were calculated. The organism was also grown in chemostat culture on H2 and sulfite, H2 and thiosulfate, and pyruvate (without sulfate).
was found to be 35 g/mol,
36 g/mol, and Y
pyr
max
10 g/mol. The growth yields are discussed with respect to ATP gains in dissimilatory sulfate reduction. 相似文献
11.
R. Stephenson B. K. Evans D. R. Jones 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1996,166(1):46-54
Maximum submergence time of Canada geese was 18% of that of similarly sized Pekin ducks. Due to a smaller respiratory system volume the oxygen store of Canada geese was 82% of that of Pekin ducks, accounting for approximately 33% of the difference in underwater survival times. The respiratory properties and volume of the blood were similar in both species. Both species utilised approximately 79% of the respiratory oxygen store and 90% of the blood oxygen store. Therefore, most of the species difference in survival times was due to a less effective oxygen-conserving cardiovascular response (bradycardia, peripheral vasoconstriction) in Canada geese. Duck cardiac chronotropic sensitivity to hypoxia during submergence was twice that observed in geese. Furthermore, a lower hypoxic ventilatory response was observed in geese than in ducks. Density of monoamine varicosities in hindlimb artery walls was lower in geese than ducks. However, electrical stimulation of the hindlimb muscles did not cause ascending vasodilation during submergence in either species, perhaps due to higher levels of catecholamines in submerged geese. We conclude that the major difference between species is higher oxygen chemosensitivity in ducks which effects a much more rapid and efficacious oxygen-conserving response during forced submergence.Abbreviations ATPS · BTPS · STPD
CNS central nervous system
-
EEG
electroencephalogram
-
ECG
electrocardiogram
-
EDTA
ethylenediaminetetra-acetic acid
-
HPLC
high performance liquid chromatography
-
fractional oxygen concentration of inspired air
-
pre-immersion fractional concentration of oxygen in the respiratory system
-
pre-emersion fractional concentration of oxygen in the respiratory system
- [Hb]
haemoglobin concentration
-
Hct
haematocrit
-
HR
heart rate
-
M
B
body mass
-
M
b
brain mass
-
M
h
heart mass
-
partial pressure of carbon dioxide in arterial blood
-
partial pressure of oxygen in arterial blood
-
SPG
sucrose-potassium phosphate-glyoxylic acid
-
t
d
maximum underwater survival time
-
respiratory minute volume
-
V
pl
plasma volume
-
V
rs
respiratory system volume
-
accessible respiratory system oxygen store
-
total non-myoglobin-bound oxygen store
-
V
tb
blood volume
-
blood oxygen store 相似文献
12.
Jürgen M. Schmidt Richard R. Ernst Saburo Aimoto Masatsune Kainosho 《Journal of biomolecular NMR》1995,6(1):95-105
Summary A simple heteronuclear relayed E.COSY pulse sequence with a minimum number of pulses is proposed for the quantitative determination of heteronuclear three-bond J-coupling constants in uniformly 13C-enriched polypeptide samples. Numerous heteronuclear three-bond coupling constants, including
,
,
, and
, can be determined for each residue from a single heteronuclear relayed E.COSY spectrum. Couplings relevant for stereospecific assignments as well as for the determination of dihedral angles in the amino acid backbone and in side chains are obtained. The method is demonstrated on the uniformly 13C-enriched decapeptide antamanide (-Val1-Pro2-Pro3-Ala4-Phe5-Phe6-Pro7-Pro8-Phe9-Phe10-). 相似文献
13.
High groundwater nitrate concentrations inhibit eutrophication of sulphate-rich freshwater wetlands 总被引:2,自引:0,他引:2
E.C.H.E.T. Lucassen A.J.P. Smolders A.L. van der Salm J.G.M. Roelofs 《Biogeochemistry》2004,67(2):249-267
During the last 60 years, pollution of the groundwater with
has greatly increased in many parts of Europe, as a consequence of excessive use of manure and synthetic fertilisers. Monitoring of groundwater-fed wetlands indicated that sediments with high
concentrations had the lowest Fe and
concentrations in the pore water. A comparison of two restored open water fens, differing in
supply via the groundwater, indicated that the redox potential and the sulphate (
) reduction rate were lower when the groundwater contained not only
but also high
concentrations. The lower
reduction rates in the
-rich open water fen were associated with lower
concentrations and the presence of plant species characteristic of clear water. In contrast, the higher
reduction rates in the
-poor open water fen were associated with very high
concentrations and massive development of plant species characteristic of eutrophic environments. Investigations at
-rich seepage sites in black alder carrs, showed that high
concentrations in the pore water caused chlorosis in the alder carr vegetation, due to lower availability of Fe in the pore water and less Fe uptake by the plants. Experimental desiccation of sediments proved that the
-rich seepage sites contained no oxidisable FeS
x
, contrary to
-poor locations, which became acidified and mobilised extremely high amounts of
due to FeS
x
oxidation. A laboratory experiment showed that
addition to sediments led to reduced releases of Fe,
and S2–, very likely due to the oxidation of reduced Fe and S compounds. Overall, the results confirmed that
is an energetically more favourable electron acceptor in anaerobic sediments than Fe and
, and that high
loads function as a redox buffer, preventing reduction of Fe and
. Limited
reduction prevents S2– -mediated mobilisation of
from Fe-
complexes. At a higher redox potential, reduced Fe, including FeS
x
, was oxidised, increasing the content of Fe(III) capable of binding
. This prevented increased
availability and the concomitant massive development of plant species characteristic of eutrophic environments. 相似文献
14.
D. L. Evans R. J. Rose 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1988,157(6):791-799
Summary Six Standardbred horses were used to evaluate the time course of pulmonary gas exchange, ventilation, heart rate (HR) and acid base balance during different intensities of constant-load treadmill exercise. Horses were exercised at approximately 50%, 75% and 100% maximum oxygen uptake (
max) for 5 min and measurements taken every 30 s throughout exercise. At all work rates, the minute ventilation, respiratory frequency and tidal volume reached steady state values by 60 s of exercise. At 100%
max, the oxygen consumption (
) increased to mean values of approximately 130 ml/kg·min, which represents a 40-fold increase above resting
. At the low and moderate work rates,
showed no significant change from 30 s to 300 s of exercise. At the high work rate, the mean
at 30 s was 80% of the value at 300 s. The HR showed no significant change over time at the moderate work rate but differing responses at the low and high work rates. At the low work rate, the mean HR decreased from 188 beats/min at 30 s to 172 beats/min at 300 s exercise, whereas at the high work rate the mean HR increased from 204 beats/min at 30 s to 221 beats/min at 300 s exercise. No changes in acid base status occurred during exercise at the low work rate. At the moderate work rate, a mild metabolic acidosis occurred which was nonprogressive with time, whereas the high work rate resulted in a progressive metabolic acidosis with a base deficit of 16 mmol/l by 300 s exercise. It is concluded that the kinetics of gas exchange during exercise are more rapid in the horse than in man, despite the relatively greater change in
in the horse when going from rest to high intensity exercise.Symbols and abbreviations E
minute ventilation
-
V
T
tidal volume
-
oxygen uptake
-
carbon dioxide output
-
oxygen pulse
-
ventilatory equivalent for oxygen
-
ventilatory equivalent for carbon dioxide
- R
respiratory exchange ratio
- HR
heart rate
- SBC
standard bicarbonate
- STPD
standard temperature and pressure dry
- BTPS
body temperature and pressure saturated
-
arterial oxygen content
-
arteriovenous oxygen content difference
- Rf
respiratory frequency 相似文献
15.
Uwe Hoffmann Dieter Eβfeld Dieter Leyk Hans-Georg Wunderlich Jürgen Stegemann 《European journal of applied physiology and occupational physiology》1994,69(2):93-97
Power-oxygen uptake (
) frequency responses can be used to predict
responses to arbitrary exercise intensity patterns. It is still an open question for which range of exercise intensities such computed
response patterns yield valid predictions. In the present study, we determined the power-
frequency response of nine sports students by means of pseudo-randomised switching between 20 W and 80 W during upright and supine cycle exercise. Starting from a baseline of 20 W each subject also performed sustained step increases to 40 W, 80 W, 120 W, and 160 W in both positions. The individual
step responses were then compared with the expected
time-courses predicted on the basis of the individual
frequency responses. The comparison showed a close agreement for the 20 W–40 W and 20 W–80 W steps in both positions. With larger step amplitudes the
kinetics became increasingly slower than the predicted
time course in both positions. During additional ramp tests (10 W · 30 s–1) whole blood lactic acid concentration [1a–]b tended to be higher in the supine position at exercise intensities higher than 160 W. The mean power at 4 mmol · 1–1 [la–]b amounted to 234 (SD 32) W and 253 (SD 44) W (P<5%) in the supine and the upright position, respectively. The maximal oxygen uptake relative to body mass was not found to be significantly different [upright, mean 57 (SD 10) ml · (min · kg)–1;supine, mean 54 (SD 10) ml · (min · kg)]. These findings would suggest that for a range of mild exercise intensities
kinetics are not appreciably influenced by the step amplitude or by cardiovascular changes associated with the upright and the supine position. 相似文献
16.
Veronique Billat Jean C. Renoux Jacques Pinoteau Bernard Petit Jean P. Koralsztein 《European journal of applied physiology and occupational physiology》1994,69(3):271-273
The aim of this study was to measure running times to exhaustion (Tlim) on a treadmill at 100% of the minimum velocity which elicits
max
max in 38 elite male long - distance runners
max = 71.4 ± 5.5 ml.kg–1.min–1 and
max = 21.8 ± 1.2 km.h–1). The lactate threshold (LT) was defined as a starting point of accelerated lactate accumulation around 4 mM and was expressed in
max. Tlim value was negatively correlated with
max (r = -0.362, p< 0.05) and
max (r = –0.347, p< 0.05) but positively with LT (%v
max) (r = 0.378, p < 0.05). These data demonstrate that running time to exhaustion at
max in a homogeneous group of elite male long-distance runners was inversely related to
max and experimentally illustrates the model of Monod and Scherrer regarding the time limit-velocity relationship adapted from local exercise for running by Hughson et al. (1984) . 相似文献
17.
Methanobacterium thermoautotrophicum was grown in continuous culture in a fermenter gassed with H2 and CO2 as sole carbon and energy sources, and in a medium which contained either NH4Cl or gaseous N2 as nitrogen source. Growth was possible with N2. Steady states were obtained at various gas flow rates with NH4Cl and with
and the maintenance coefficient varied with the gas input and with the nitrogen source. Growth of Methanococcus thermolithotrophicus in continuous culture in a fermenter gassed with H2, CO2 as nitrogen, carbon and energy sources was also examined.Abbreviations
molecular growth yield (g dry weight of cells per mol of CH4 evolved)
-
growth rate (h-1)
-
D
dilution rate (h-1)
- rate (h-1);
relation of Neijssel and Tempest and of Stouthamer and Bettenhaussen
-
energy 相似文献
18.
Summary Nitrate reductase (EC 1.6.6.2) activity (NRA), as measured by an in vivo assay, is present in needle leaves and mycorrhizal fine root tips of adult Norway spruce [Picea abies (L.) Karst.] in at least equal amounts on a fresh weight basis, in both adult and 5-year-old trees. NRA could also be demonstrated in trunk wood of deroted trees after fertilization with 5 mM
, exhibiting a longitudinal profile in the trunk. Inducibility in needles can more efficiently be achieved by NO2 (100 g·m-3) than by 5 mM nitrate, which is effective only in root-amputated trees. A remarkably high level of needle-NRA in unfertilized trees, which are characterized by a very low level of nitrate in the xylem sap, suggests that NRA in spruce needles may in part be constitutive. Organic-N is a major nitrogen source for the needles even in root-amputated trees, indicating pronounced exchange processes between ray parenchyma and trunk xylem, which in turn are modified by the nitrogen source fed to the trunk stump. Intact trees exhibit a very similar amino acid composition of the xylem sap, regardless of whether
or
has been fed. The amino acid pattern of the needles is not thrown out of balance by flooding with
and
, which occurs in fertilized derooted trees. This indicates a distinct potential for homoeostasis of nitrogen entrance-metabolism (i.e. NRA and glutamine synthetase activity) in the needles. In the ectomycorrhiza/fine root-system (EMC), marked differences in NRA were observed depending on root-tip diameter and along the longitudinal profile of the fine roots. EMC-nitrate reductase is strongly enhanced by
. Needle-NRA exhibits a circannual rhythm. An early summer maximum is followed by a December minimum. This activity pattern matches well the transitory increase of soluble nitrogen in spring and the total protein maximum in winter. In an indirect way assimilatory NRA may well contribute to nitrogen overfertilization (by consumption of NOX) as one possible cause of the contemporary decline of spruce populations. 相似文献
19.
Gill function in an elasmobranch 总被引:1,自引:0,他引:1
Gordon C. Grigg John Read 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1971,73(4):439-451
Summary Highly efficient oxygen uptake in elasmobranchs, as indicated by frequent excess of
over
has previously been ascribed to the operation of multicapillary rather than counter-current gas exchange by the gills. Analysis of models shows that, at maximum efficiency, a multicapillary system cannot account for values of
greater than
. In Port Jackson sharks Heterodontus portusjacksoni)
commonly exceeds
, which indicates the operation of a functional counter-current at the respiratory surface. The anatomical basis of this counter-current is provided by the demonstration that a continuous flow of water passes between the secondary lamellae into septal canals and thence via the parabranchial cavities to the exterior.Queen Elizabeth II Fellow. 相似文献
20.
The tetrameric heart isozyme of lactate dehydrogenase (H4) is modified by p-chloromercuribenzoate (PCMB) to produce the inactive tetramer
and then hybridized with native tetrameric muscle isozyme (M4). The hybrid mixture
was isolated by polyacrylamide gel electrophoresis (PAGE) and then stained for enzyme activity and with Coomassie brilliant blue. Only three bands were found on the gels in either case. The hybrid enzymes
as isolated by PAGE have half the specific activity of the native muscle enzyme. The electrophoresis properties of HM3 are very similar to those of HM3, while the electrophoresis properties of
are very similar to those of H2M2. The above results strongly suggest that the tetramer having enzymatic activity contains at least two native subunits, and the di-subunit in the tetrameric enzyme is the minimal functional unit. 相似文献