COMBINED REPLACEMENT OF THE AORTIC VALVE AND ASCENDING AORTA IN JEHOVAH'S WITNESSES: REPORT OF TWO CASES

Two Jehovah's Witnesses with large ascending thoracic aortic aneurysms and aortic insufficiency secondary to annuloaortic ectasia underwent successful combined replacement of the aortic valve and the ascending aorta. One patient received a composite graft containing an aortic valve prosthesis, which necessitated supravalvular coronary ostia reimplantation; the other patient underwent separate aortic valve and left supracoronary ascending aneurysm replacement, with reimplantation of the right coronary ostium into the graft. No blood or blood derivatives were administered. Both patients had uneventful recoveries and continue to do well. To our knowledge, they represent the first reported cases of successful combined replacement of the aortic valve and ascending aorta in Jehovah's Witnesses.     

AORTIC PUNCH: AN ADJUNCT FOR CANNULATION OF THE ASCENDING AORTA DURING EXTRACORPOREAL CIRCULATION

A new aortic punch has been designed for use during cannulation of the ascending aorta. The instrument is extremely safe, and it effectively shortens the steps required for aortic cannulation.     

SURGICAL CONSIDERATIONS IN DISEASES OF THE PANCREAS

Present data indicate that surgical treatment of pancreatitis is most successful in cases in which the disease is caused by extrahepatic disease of the biliary tract.Operation is also effective in cases of islet cell tumors, cysts, lithiasis, trauma and annular pancreas.Results seem to justify radical operation for early carcinoma of the ampulla of Vater and pancreas.The value of surgical treatment in other lesions of the pancreas is doubtful.     

猫背外侧索中上行纤维的电生理学特征的初步观察

在麻醉猫的脊髓的背外侧索中用显微解剖法剥制出神经纤维细束,研究其传入的单位放电活动。根据对自然刺激的反应共记录到五类性质的不同的单位:肌梭传入单位(n=111),对轻压刺激敏感的低阈值机械感受单位(n=85),对触毛十分敏感的毛单位(n=65),毛—低阈值机械感受性混合单位(n=17),以及仅对伤害性刺激有反应的高阈值机械感受性单位(n=11)。其中肌梭单位最易观察到,可能是肌梭的传入纤维的直径较粗,因而较易完整无损地被分离到。值得指出,所有传递躯体感受信息的脊髓上行纤维均位于背外侧索的外侧三分之一处,而其他纤维则主要分布于背外侧索的内侧三分之二的区域。     

CHROMOSOMAL RELATIONSHIPS AND TAXONOMIC CONSIDERATIONS IN THE GENUS CASSIA

Irwin , H. S. and B. L. Turner . (U. Texas, Austin.) Chromosomal relationships and taxonomic considerations in the genus Cassia. Amer. Jour. Bot. 47(4): 309-318. 1960.—All chromosome numbers known for the genus Cassia are arranged in a table which follows the infrageneric organization of Bentham (1871). Included are new counts for 88 taxa, of which 68 are first reports. Evidence is represented which suggests that the base number for the genus is 7, the other numbers having been derived by aneuploid loss from various euploid levels. The basic number 8, of general occurrence in the Subsection Leiocalyx, Section Chameacrista, is presumed to have been derived from the ancestral generic base by aneuploid gain of 1 chromosome. The results of the present study do not support elevation of the section Chamaecrista to generic rank. In general, the basic soundness of Bentham's revision is upheld.     

CHEMICAL AND MORPHOLOGICAL STUDIES ON THE IN VITRO CALCIFICATION OF AORTA

After a lag period, rat aortas incubated in rat serum in vitro accumulated substantial quantities of calcium and phosphate. Examination of the tissue by x-ray diffraction, microradiography, electron diffraction, and electron microscopy indicated that the calcium-phosphate phase which formed was hydroxyapatite and that the crystals were localized almost exclusively in elastin. Selective elimination of various components of the aorta with proteolytic enzymes indicated that the presence of elastin was required for mineralization. Collagen fibers did not appear to be required for the initiation of calcification, nor did they seem to undergo appreciable calcification in the time periods studied. Analysis of the initial lag period suggested that at least two changes occurred in serum prior to the mineralization of this tissue. Inhibitors of the reaction were destroyed, and the level of dialyzable calcium was elevated owing to its release from serum protein.     

大鼠降主动脉雌激素受体的免疫组织化学研究

冰冻切片微波修复后,用ER单克隆抗体免疫组织化学SP法及图像分析方法,观察雌激素受体（ER）在雌性与雄性大鼠降主动脉壁的分布,结果显示,ER阳性反应存在于血管壁的内皮细胞与平滑肌细胞中,阳性反应强度无性别差异。无段间差异（降主动脉胸段与腹段）,血管周围的脂肪组织也可见大量呈阳性反应的细胞,结果表明,降主动脉壁的内皮细胞,血管平滑肌细胞中存在ER。降主动脉可能是雌激素的靶器官。     

GRAFT SIZE CONSIDERATIONS IN THE KINETICS OF SPLEEN COLONY DEVELOPMENT

The kinetics of spleen colony development has been studied after the injection of 10⁶, 10⁵ and 3 × 10⁴ bone marrow cells. The results indicate that:

• 1 The CFU population growth rate is independent of cell dose until the logarithmic growth phase is passed. Slowing of growth was seen by day 12 after the highest dose, by day 15 after the median dose, but was not observed during the period of observation after the low dose.
• 2 The growth rate of CFU per colony is independent of cell dose, but the curves are not identical. The differences between the curves leads to the conclusion that there is a dose-dependent delay in the commencement of CFU proliferation. The delay is roughly equal to one cell cycle time between the medium and high inoculum groups and also between the medium and low inoculum groups.
• 3 The number of cells per colony is graft size dependent, the doubling times, where these can be roughly assessed, being inversely related to the graft size. From the average number of cells per colony on day 6 it is calculated that the mean doubling time in the early stages of colony development is less than 7 hr.
• 4 The proportion CFU:colony cells is dose dependent with the highest inoculum having the highest proportion and the low inoculum group having the lowest proportion.