串叶松香草高效再生体系的建立与兔出血症病毒YL株外壳蛋白基因VP60对其遗传转化

通过对串叶松香草(Silphium perfoliatumL.)不同激素浓度配比的诱导分化实验,建立了串叶松香草离体培养高效再生体系,结果表明MS 6-BA(2.0mg/L) NAA0.1(mg/L)培养基可高效诱导愈伤组织和芽的分化,1/2MS IBA(0.1mg/L)培养基可快速诱导根的生成,形成再生植株。构建了植物表达载体pBI121-VP60,利用根癌农杆菌(Agrobacterium tumefaciens)介导叶盘法转化串叶松香草以研究高效的串叶松香草转化体系,结果显示以农杆菌LBA4404为介导菌株、以叶片为转化外植体、3d预培养时间和3～4d共培养时间、400mg/L羧苄青霉素和40mg/L卡那霉素筛选浓度转化效果较好,并已筛选到两株拟转基因植株,为利用串叶松香草生产兔出血症病毒动物可食用疫苗建立了初步的技术基础。     

串叶松香草的新用途——提取SOD开发叶蛋白

串叶松香草(Silphium Perfolialum)系菊科多年生草本植物,栽培简单,产量高,可达亩产鲜草10-12吨/年。从其营养成分的分析表明:串叶松香草含17种氨基酸,叶蛋白含量高,是一种值得得推广的重要经济作物。本文就以从该植物中提取SOD开发叶蛋白的可行性及前景作一简要介绍。     

串叶松香草的药用成分分析

本文对串叶松香草的药用成分作了定性分析,并对松香草中的核黄素和单宁作了较深入的探讨,结合用该草作的抗疲劳试验和各地的饲喂实践,可以看出,串叶松香草是一种既有高营养价值,又有多种药物疗效的优质饲草。     

串叶松香草的新用途---提取SOD开发叶蛋白

串叶松香草（Silphium Perfolialum）系菊科多年生草本植物,栽培简单,产量高,可达亩产鲜草10-12吨/年。从其营养成分的分析表明：串叶松香草含17种氨基酸,叶蛋白含量高,是一种值得得推广的重要经济作物。本文就以从该植物中提取SOD开发叶蛋白的可行性及前景作一简要介绍。     

串叶松香草多倍体植株的组织培养

植物名称:串叶松香草(Silphium perfolia—tum)。材料类别:幼苗的胚轴。培养条件:将种子用水浸泡12小时后移至500ppm的秋水仙素溶液处理12小时,然后种置于砂基培养基内,室内培养。待其长出幼苗时,取其膨大的胚轴部分接种于培养基上,置于25±0.5℃的培     

灵香草的一种严重病害调查研究初报

<正> 灵香草的斑枯病俗称“点蜡烛”病,历来是灵香草的毁灭性病害。此病分布于广西金秀县各个灵香草产区;融水、龙胜等灵香草产地亦有此病发生。一般发病率60～70％,严重的往往达80％～90％,甚至失收。如1980年8月,金秀县外贸局为扶植生产队发展灵香草生产,无偿拨给金秀二队种苗300斤,分给社员种植。灵香草生长到高约23—27厘米时,全部病死腐烂。因此,要发展灵香草生产,必须有效地防治这种病害。     

串叶松香草

串叶松香草(Silphiam perfoliatum)是原产加拿大和美国南部、西部的菊科草本植物。因它的根带有松香味,茎上部叶片的基部相连成杯状,茎杆从中贯串而出,而得名为串叶松香草。1979年引入我国。 (一)植物学特征多年生草本。株高2—3米。根为直根系。茎直立,四棱形,光滑无毛,上部分枝。叶对生,卵形,长15—20厘米,宽10—20厘米,先端急尖,下部叶基部渐狭成柄,边缘具齿,两面具糙柔毛。头状花序,在茎顶成伞房状,直径5—7.5厘米;总苞苞片数层,覆瓦状排列;舌状花黄色,2—3轮,舌片先端具3齿,能育;管状花黄色,两性,不育。瘦果,扁而具翅,呈倒卵形;冠毛芒状。 (二)生长习性在适宜条件下,充实饱满的种子播种后6—10天幼苗出土,两片子叶呈椭圆形。第一年长势缓慢,只进行营养生长,形成基生叶丛,一般12—18片,水肥条件充分时可达30片叶。不抽茎开花。第二年春季萌芽后,长势明显增强。华北地区,6月中旬     

灵香草生物学及栽培生态学特性的研究

本文用3年定点观测资料,分析了灵香草植株生长、干物质积累的进程,定量地论述了灵香草生长发育与光、温、水等生态因子之间的关系,总结了灵香草的高产栽培措施,对灵香草的引种、施肥、管理、抚育、采收等具有参考价值,试验观测方法也可供研究特种经济作物时借鉴。     

抗肿瘤药材—细梗香草规范化种植(GAP)初探

依据国家中药材生产质量管理规范(GAP)为指导原则,经课题组近几年试验研究,初步总结出细梗香草规范化种植(GAP)技术。从提高药材产量、保证药材质量出发,规范了细梗香草各生产环节和生产全过程,为促进细梗香草种植标准化、规范化提供有益的资料,为抗肿瘤新药的研究打下良好的基础。     

世界烹调香草

烹调香草逐渐受到人们的关注,用作烹调香草的植物种类很多,一些烹调香草也是药用植物,本文对世界主要烹调香草进行了综述,为进一步开发利用提供依据。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.