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1.
Crop domestication has been inferred genetically from neutral markers and increasingly from specific domestication-associated loci. However, some crops are utilized for multiple purposes that may or may not be reflected in a single domestication-associated locus. One such example is cultivated flax (Linum usitatissimum L.), the earliest oil and fiber crop, for which domestication history remains poorly understood. Oil composition of cultivated flax and pale flax (L. bienne Mill.) indicates that the sad2 locus is a candidate domestication locus associated with increased unsaturated fatty acid production in cultivated flax. A phylogenetic analysis of the sad2 locus in 43 pale and 70 cultivated flax accessions established a complex domestication history for flax that has not been observed previously. The analysis supports an early, independent domestication of a primitive flax lineage, in which the loss of seed dispersal through capsular indehiscence was not established, but increased oil content was likely occurred. A subsequent flax domestication process occurred that probably involved multiple domestications and includes lineages that contain oil, fiber, and winter varieties. In agreement with previous studies, oil rather than fiber varieties occupy basal phylogenetic positions. The data support multiple paths of flax domestication for oil-associated traits before selection of the other domestication-associated traits of seed dispersal loss and fiber production. The sad2 locus is less revealing about the origin of winter tolerance. In this case, a single domestication-associated locus is informative about the history of domesticated forms with the associated trait while partially informative on forms less associated with the trait. 相似文献
2.
The polymorphic major histocompatibility complex (MHC) has gained a specific relevance in pathogen resistance and mate choice.
Particularly the antigen-binding site (ABS), encoded by exon 2 of the DRB class II gene, exhibits numerous alleles and extensive sequence variations between alleles. A lack of MHC variability has
attributed to instances such as bottleneck effects or relaxed selection pressure and has a certain impact on the long-term
viability of the species concerned. As a result of seriously decreased population density during the last century, the current
population of the endangered European mink (Mustela lutreola, L. 1761) has suffered from geographic isolation. In this study, we amplified a partial sequence of the MHC class II DRB exon 2 (229 bp), assessed the degree of genetic variation and compared the variability with those of other Mustelidae. As
a result, nine alleles were detected in 20 investigated individuals, which differ from each other by four to 25 nucleotide
substitutions (two to 11 amino acid substitutions). Whilst an equal ratio for synonymous and non-synonymous substitutions
was found inside the ABS, synonymous substitutions were significantly higher than non-synonymous substitutions in the non-ABS
region. Results might indicate that no positive selection exists within the ex situ population of M. lutreola, at least in the analysed fragment. In addition, phylogenetic analyses support the trans-species model of evolution.
Becker and Nieberg have contributed equally to this work. 相似文献
3.
Sequence variation at the major histocompatibility complex DRB loci in beluga (Delphinapterus leucas) and narwhal (Monodon monoceros) 总被引:2,自引:2,他引:0
The variation at loci with similarity to DRB class II major histocompatibility complex loci was assessed in 313 beluga collected from 13 sampling locations across North
America, and 11 narwhal collected in the Canadian high Arctic. Variation was assessed by amplification of exon 2, which codes
for the peptide binding region, via the polymerase chain reaction, followed by either cloning and DNA sequencing or single-stranded
conformation polymorphism analysis. Two DRB loci were identified in beluga: DRB1, a polymorphic locus, and, DRB2, a monomorphic locus. Eight alleles representing five distinct lineages (based on sequence similarity) were found at the
beluga DRB1 locus. Although the relative number of alleles is low when compared with terrestrial mammals, the amino acid variation found
among the lineages is moderate. At the DRB1 locus, the average number of nonsynonymous substitutions per site is greater than the average number of synonymous substitutions
per site (0.0806 : 0.0207, respectively;P<0.01). Most of the 31 amino acid substitutions do not conserve the physiochemical properties of the residue, and 21 of these
are located at positions implicated as forming pockets responsible for the selective binding of foreign peptide side chains.
Only DRB1 variation was examined in 11 narwhal, revealing a low amount of variation. These data are consistent with an important role
for the DRB1 locus in the cellular immune response of beluga. In addition, the ratio of nonsynonymous to synonymous substitutions is similar
to that among primate alleles, arguing against a reduction in the balancing selection pressure in the marine environment.
Two hypotheses may explain the modest amount of Mhc variation when compared with terrestrial mammals: small population sizes at speciation or a reduced neutral substitution
rate in cetaceans.
Received: 15 July 1997 / Revised: 24 March 1998 相似文献
4.
The cytochrome c oxidase subunit 2 gene (COII) encodes a highly conserved protein that is directly responsible for the initial transfer of
electrons from cytochrome c to cytochrome c oxidase (COX) crucial to the production of ATP during cellular respiration. Despite its integral role in electron transport,
we have observed extensive intraspecific nucleotide and amino acid variation among 26 full-length COII sequences sampled from
seven populations of the marine copepod, Tigriopus californicus. Although intrapopulation divergence was virtually nonexistent, interpopulation divergence at the COII locus was nearly 20%
at the nucleotide level, including 38 nonsynonymous substitutions. Given the high degree of interaction between the cytochrome
c oxidase subunit 2 protein (COX2) and the nuclear-encoded subunits of COX and cytochrome c (CYC), we hypothesized that some codons in the COII gene are likely to be under positive selection in order to compensate
for amino acid substitutions in other subunits. Estimates of the ratio of nonsynonymous to synonymous substitution (ω), obtained
using a series of maximum likelihood models of codon substitution, indicated that the majority of codons in T. californicus COII are under strong purifying selection (ω << 1), while approximately 4% of the sites in this gene appear to evolve under
relaxed selective constraint (ω = 1). A branch-site maximum likelihood model identified three sites that may have experienced
positive selection within the central California sequence clade in our COII phylogeny; these results are consistent with previous
studies showing functional and fitness consequences among interpopulation hybrids between central and northern California
populations.
[Reviewing Editor: Dr. Willie Swanson] 相似文献
5.
Ashley MV Wilk JA Styan SM Craft KJ Jones KL Feldheim KA Lewers KS Ashman TL 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,107(7):1201-1207
The objectives of the present study were to develop microsatellite markers for the wild strawberry, Fragaria virginiana, to evaluate segregation patterns of microsatellite alleles in this octoploid species, and assess genetic variability at microsatellite loci in a wild population. A genomic library was screened for microsatellite repeats and several PCR primers were designed and tested. We also tested the use of heterologous primers and found that F. virginiana primers amplified products in cultivated strawberry, Fragaria × ananassa Duch. and Fragaria chiloensis. Similarly, microsatellite loci developed from cultivated strawberry also successfully amplified F. virginiana loci. We investigated four microsatellite loci in detail, three developed from F. virginiana and one from cultivated strawberry. A survey of 100 individuals from a population of F. virginiana in Pennsylvania demonstrated high heterozygosities (He or gene diversity ranged from 0.80 to 0.88 per locus) and allelic diversity (12–17 alleles per locus), but individual plants had no more than two alleles per locus. Segregation patterns in parents and progeny of two controlled crosses at these four loci were consistent with disomic Mendelian inheritance. Together these findings suggest that the genome of F. virginiana is "highly diploidized" and at least a subset of microsatellite loci can be treated as codominant, diploid markers. Significant heterozygote deficiencies were found at three of the four loci for hermaphroditic individuals but for only one locus among females in this gynodioecious species.Communicated by J. Dvorak 相似文献
6.
Although white-tailed deer (Odocoileus virginianus) are one of North America's best studied mammals, no information is available concerning allelic diversity at any locus of
the major histocompatibility complex in this taxon. Using the polymerase chain reaction, single-stranded conformation polymorphism
analysis, and DNA sequencing techniques, 15 DRB exon 2 alleles were identified among 150 white-tailed deer from a single population in southeastern Oklahoma. These alleles
represent a single locus and exhibit a high degree of nucleotide and amino acid polymorphism, with most amino acid variation
occurring at positions forming the peptide binding sites. Furthermore, twenty-seven amino acid residues unique to white-tailed
deer DRB alleles were detected, with 19 of these occurring at residues forming contact points of the peptide binding region. Significantly
higher rates of nonsynonymous than synonymous substitutions were detected among these DRB alleles. In contrast to other studies of Artiodactyla DRB sequences, interallelic recombination does not appear to be playing a significant role in the generation of allelic diversity
at this locus in white-tailed deer. To examine evolution of white-tailed deer (Odvi-DRB) alleles within Cervidae, we performed a phylogenetic analysis of all published red deer (Ceel-DRB), roe deer (Caca-DRB), and moose (Alal-DRB) DRB alleles. The phylogenetic tree clearly shows a trans-species persistence of DRB lineages among these taxa. Moreover, this phylogenetic tree provides insight into evolution of DRB allelic lineages within Cervidae and may aid in assignment of red deer DRB alleles to specific loci.
Received: 25 June 1998 / Revised: 2 September 1998 相似文献
7.
YB Fu 《Ecology and evolution》2012,2(3):622-635
Cultivated flax (Linum usitatissimum L.) is the earliest oil and fiber crop and its early domestication history may involve multiple events of domestication for oil, fiber, capsular indehiscence, and winter hardiness. Genetic studies have demonstrated that winter cultivated flax is closely related to oil and fiber cultivated flax and shows little relatedness to its progenitor, pale flax (L. bienne Mill.), but winter hardiness is one major characteristic of pale flax. Here, we assessed the genetic relationships of 48 Linum samples representing pale flax and four trait-specific groups of cultivated flax (dehiscent, fiber, oil, and winter) through population-based resequencing at 24 genomic regions, and revealed a winter group of cultivated flax that displayed close relatedness to the pale flax samples. Overall, the cultivated flax showed a 27% reduction of nucleotide diversity when compared with the pale flax. Recombination frequently occurred at these sampled genomic regions, but the signal of selection and bottleneck was relatively weak. These findings provide some insight into the impact and processes of flax domestication and are significant for expanding our knowledge about early flax domestication, particularly for winter hardiness. 相似文献
8.
We analyzed the polymorphic nature of eleven alleles expressed by the major class I locus (B-FIV) in chickens. Similar to mammalian class I loci, the nucleotide substitutions with high variability occur in exons 2 and
3 encoding the α1 and α2 domains. However, the nonsynonymous to synonymous ratio of nucleotide substitutions in exon 3 encoding
the α helix and β sheets is reversed compared with HLA. The region of exon 3 encoding the α2 helix demonstrates a much lower
nonsynonymous to synonymous ratio, suggesting evolutionary selection of a more conserved α2 helix in B-FIV compared with HLA.
Amino acid residues with high Wu-Kabat variability are typically located in positions predicted to impact antigen presentation.
B-FIV amino acid residues predicted to interact with the CDR1α region of the T-cell receptor (Tcr) demonstrate less variability
than in mouse and human class I alleles. The combination of a reduced nonsynonymous to synonymous ratio in exon 3 encoding
the α2 helix and the limited variability in CDR1α contact residues is discussed with regard to concerted evolution between
a minimal major histocompatibility complex and compaction of Tcr variable gene segments in the chicken.
Received: 18 Juli 1997 / Revised: 21 November 1997 相似文献
9.
R. S. Pfau Ronald A. Van Den Bussche Karen McBee Robert L. Lochmiller 《Immunogenetics》1999,49(10):886-893
The cotton rat (Sigmodon hispidus) is a common murid rodent of the southern United States, Mexico, and Central America. Using single-stranded conformation
polymorphism analysis and DNA sequencing techniques, 11 DQA exon 2 alleles were detected among 180 S. hispidus from Caddo County, Oklahoma, USA. The alleles represent a single locus exhibiting a high level of polymorphism. Nucleotide
and amino acid distance values among DQA alleles of S. hispidus were higher than those within Mus musculus and species of Rattus. Although the distribution of polymorphic amino acid residues among alleles of S. hispidus was similiar to that of Mus and Rattus, some residues of the α-helix region were more variable in S. hispidus. Comparisons of nonsynonymous and synonymous substitutions indicated a trend toward higher numbers of nonsynonymous substitutions;
however, this difference was not significant statistically among S. hispidus alleles. To examine evolution of DQA alleleswithin Muridae, we performed a phylogenetic analysis that included DQA alleles from S. hispidus, Peromyscus leucopus, M. musculus, R. norvegicus, and six Australian species of Rattus. Results depicted monophyly for each genus, and this concordance between species and gene trees represents a lack of evidence
for trans-species persistence of alleles among these genera.
Received: 22 October 1998 / Revised: 17 March 1999 相似文献
10.
Sällström B Arnaout RA Davids W Bjelkmar P Andersson SG 《Journal of molecular evolution》2006,62(5):600-614
In free-living microorganisms, such as Escherichia coli and Saccharomyces cerevisiae, both synonymous and nonsynonymous substitution frequencies correlate with expression levels. Here, we have tested the hypothesis
that the correlation between amino acid substitution rates and expression is a by-product of selection for codon bias and
translational efficiency in highly expressed genes. To this end, we have examined the correlation between protein evolutionary
rates and expression in the human gastric pathogen Helicobacter pylori, where the absence of selection on synonymous sites enables the two types of substitutions to be uncoupled. The results revealed
a statistically significant negative correlation between expression levels and nonsynonymous substitutions in both H. pylori and E. coli. We also found that neighboring genes located on the same, but not on opposite strands, evolve at significantly more similar
rates than random gene pairs, as expected by co-expression of genes located in the same operon. However, the two species differ
in that synonymous substitutions show a strand-specific pattern in E. coli, whereas the weak similarity in synonymous substitutions for neighbors in H. pylori is independent of gene orientation. These results suggest a direct influence of expression levels on nonsynonymous substitution
frequencies independent of codon bias and selective constraints on synonymous sites.
Electronic Supplementary Material Electronic Supplementary material is available for this article at
and accessible for authorised users.
[Reviewing Editor: Dr. Nicolas Galtier] 相似文献
11.
The frequencies of alleles of killer cell immunoglobulin-like receptor genes, KIR3DL3 and KIR3DL2, and the carrier frequency of KIR2DL4 alleles have been determined from a population of African Americans (n = 100) by DNA sequencing of the coding regions. Fifty alleles of KIR3DL3 were observed with the most frequent, KIR3DL3*00901 (13%). KIR3DL2 was also diverse; 32 alleles with KIR3DL2*00103 the most frequent (17%). For KIR2DL4, of the 18 alleles observed, one allele, KIR2DL4*00103, was found in 64 of the 100 individuals. Thirty-six novel alleles encoding a total of 28 unique receptors are described.
Pairwise comparisons among all of the alleles at each locus suggest a predominance of synonymous substitutions. The variation
at all three framework loci fits a neutral model of evolution. 相似文献
12.
Worley K Gillingham M Jensen P Kennedy LJ Pizzari T Kaufman J Richardson DS 《Immunogenetics》2008,60(5):233-247
In species with duplicated major histocompatibility complex (MHC) genes, estimates of genetic variation often rely on multilocus
measures of diversity. It is possible that such measures might not always detect more detailed patterns of selection at individual
loci. Here, we describe a method that allows us to investigate classical MHC diversity in red jungle fowl (Gallus gallus), the wild ancestor of the domestic chicken, using a single locus approach. This is possible due to the well-characterised
gene organisation of the ‘minimal essential’ MHC (BF/BL region) of the domestic chicken, which comprises two differentially
expressed duplicated class I (BF) and two class II B (BLB) genes. Using a combination of reference strand-mediated conformation
analysis, cloning and sequencing, we identify nine BF and ten BLB alleles in a captive population of jungle fowl. We show
that six BF and five BLB alleles are from the more highly expressed locus of each gene, BF2 and BLB2, respectively. An excess of non-synonymous substitutions across the jungle fowl BF/BL region suggests that diversifying selection
has acted on this population. Importantly, single locus screening reveals that the strength of selection is greatest on the
highly expressed BF2 locus. This is the first time that a population of red jungle fowl has been typed at the MHC region,
laying the basis for further research into the underlying processes acting to maintain MHC diversity in this and other species. 相似文献
13.
H. K. C. Laidlaw E. S. Mace S. B. Williams K. Sakrewski A. M. Mudge P. J. Prentis D. R. Jordan I. D. Godwin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(7):1227-1237
The β-, γ- and δ-kafirin genes were sequenced from 35 Sorghum genotypes to investigate the allelic diversity of seed storage proteins. A range of grain sorghums, including inbred parents
from internationally diverse breeding programs and landraces, and three wild Sorghum relatives were selected to encompass an extensive array of improved and unimproved germplasm in the Eusorghum. A single locus
exists for each of the expressed kafirin-encoding genes, unlike the multigenic α-kafirins. Significant diversity was found for each locus, with the cysteine-rich β-kafirin having four alleles, including the first natural null mutant reported for this prolamin subfamily. This allele contains
a frame shift insertion at +206 resulting in a premature stop codon. SDS-PAGE revealed that lines with this allele do not
produce β-kafirin. An analysis of flour viscosity reveals that these β-kafirin null lines have a difference in grain quality, with significantly lower viscosity observed over the entire Rapid
ViscoAnalyser time course. There was less diversity at the protein level within the cysteine-rich γ-kafirin, with only two alleles in the cultivated sorghums. There were only two alleles for the δ-kafirin locus among the S. bicolor germplasm, with one allele encoding ten extra amino acids, of which five were methionine residues, with an additional methionine
resulting from a nucleotide substitution. This longer allele encodes a protein with 19.1% methionine. The Asian species, S. propinquum, had distinct alleles for all three kafirin genes. We found no evidence for selection on the three kafirin genes during sorghum
domestication even though the δ-kafirin locus displayed comparatively low genetic variation. This study has identified genetic diversity in all single copy
seed storage protein genes, including a null mutant for β-kafirin in Sorghum. 相似文献
14.
Evolution of hominoid mitochondrial DNA with special reference to the silent substitution rate over the genome 总被引:4,自引:0,他引:4
Rumi Kondo Satoshi Horai Yoko Satta Naoyuki Takahata 《Journal of molecular evolution》1993,36(6):517-531
Summary Focusing on the synonymous substitution rate, we carried out detailed sequence analyses of hominoid mitochondrial (mt) DNAs of ca. 5-kb length. Owing to the outnumbered transitions and strong biases in the base compositions, synonymous substitutions in mtDNA reach rapidly a rather low saturation level. The extent of the compositional biases differs from gene to gene. Such changes in base compositions, even if small, can bring about considerable variation in observed synonymous differences and may result in the region-dependent estimate of the synonymous substitution rate. We demonstrate that such a region dependency is due to a failure to take proper account of heterogeneous compositional biases from gene to gene but that the actual synonymous substitution rate is rather uniform. The synonymous substitution rate thus estimated is 2.37 ± 0.11 × 10–8 per site per year and comparable to the overall rate for the noncoding region. On the other hand, the rate of nonsynonymous substitutions differs considerably from gene to gene, as expected under the neutral theory of molecular evolution. The lowest rate is 0.8 × 10–9 per site per year forCOI and the highest rate is 4.5 × 10–9 forATPase 8, the degree of functional constraints (measured by the ratio of the nonsynonymous to the synonymous substitution rate) being 0.03 and 0.19, respectively. Transfer RNA (tRNA) genes also show variability in the base contents and thus in the nucleotide differences. The average rate for 11 tRNAs contained in the 5-kb region is 3.9 × 10–9 per site per year. The nucleotide substitutions in the genome suggest that the transition rate is about 17 times faster than the transversion rate. 相似文献
15.
Maria von Korff Huajun Wang Jens Léon Klaus Pillen 《Molecular breeding : new strategies in plant improvement》2008,21(1):81-93
Malting quality is genetically determined by the complex interaction of numerous traits which are expressed prior to and,
in particular, during the malting process. Here, we applied the advanced backcross quantitative trait locus (AB-QTL) strategy
(Tanksley and Nelson, Theor Appl Genet 92:191–203, 1996), to detect QTLs for malting quality traits and, in addition, to identify favourable exotic alleles for the improvement of
malting quality. For this, the BC2DH population S42 was generated from a cross between the spring barley cultivar Scarlett and the wild barley accession ISR42-8
(Hordeum vulgare ssp. spontaneum). A QTL analysis in S42 for seven malting parameters measured in two different environments yielded 48 QTLs. The exotic genotype
improved the trait performance at 18 (37.5%) of 48 QTLs. These favourable exotic alleles were detected, in particular, on
the chromosome arms 3HL, 4HS, 4HL and 6HL. The exotic allele on 4HL, for example, improved α-amylase activity by 16.3%, fermentability
by 0.8% and reduced raw protein by 2.4%. On chromosome 6HL, the exotic allele increased α-amylase by 16.0%, fermentability
by 1.3%, friability by 7.3% and reduced viscosity by 2.9%. Favourable transgressive segregation, i.e. S42 lines exhibiting
significantly better performance than the recurrent parent Scarlett, was recorded for four traits. For α-amylase, fermentability,
fine-grind extract and VZ45 20, 16, 2 and 26 S42 lines, respectively, surpassed the recurrent parent Scarlett. The present
study hence demonstrates that wild barley does harbour valuable alleles, which can enrich the genetic basis of cultivated
barley and improve malting quality traits. 相似文献
16.
The South China tiger (Panther tigris amoyensis) is critically endangered with 73 remaining individuals living in captivity, all derived from six wild founders since 1963.
The population shows a low level of juvenile survivorship and reproductive difficulties, and faces a huge conservation challenge.
In this study, inbreeding depression and genetic diversity decline were examined by using pedigree data and 17 microsatellites.
The constant B, which is related to the number of lethal equivalents, was estimated to be 0 for the offspring of noninbred parents, but
was >0 for the offspring of inbred parents and for all offspring. Percentage of successfully breeding tigers inversely correlated
with inbreeding level (r = −0.626, α = 0.05). Taken together, these findings suggest the population is suffering from inbreeding depression in juvenile survivorship
and fecundity. No significant correlation was detectable for the mean litter size with f of either dams (r = −0.305, α = 0.46) or kittens (r = 0.105, α = 0.71), indicating litter size was not strongly subject to inbreeding depression. The average number of alleles per locus
was 4.24 ± 1.03 (SE), but effective number of alleles was only 2.53 ± 0.91. Twenty-one alleles carried by early breeders at
13 loci were absent in the present breeders and potential breeders. Multilocus heterozygosity was inversely correlated with
inbreeding levels (r = −0.601, α = 0.004). These findings suggest rapid allelic diversity loss is occurring in this small captive population and that heterozygosity
is being lost as it becomes more inbred. Our phylogenetic analysis supports past work indicating introgression from northern
Indochinese tigers in the population. As no wild representatives of the South China tiger can be added to the captive population,
we may consider the alternate scenario of further introgression in the interest of countering inbreeding depression and declining
genetic diversity. 相似文献
17.
We tested the cross-amplification of 26 microsatellites developed for passerines and an additional three developed for Gallinula species in eight European Coots from two populations. Sixteen microsatellite markers successfully amplified, of which nine
were polymorphic with 2–6 alleles (mean 3.7 alleles) and an expected heterozygosity (H
e) ranging from 0.375 to 0.805 (mean H
e = 0.589). On average, we found 2.22 alleles/locus and a mean H
e of 0.440 in one nest, and 2.56 alleles/locus and a mean H
e of 0.494 in the other one. These nine polymorphic markers could be of potential use in studies of genetic variability, population
structure and reproductive strategy of European Coots. 相似文献
18.
Jonathan E. Boyson Kristen K. Iwanaga Julie A. Urvater Austin L. Hughes Thaddeus G. Golos D. I. Watkins 《Immunogenetics》1999,49(2):86-98
HLA-G is a nonclassical major histocompatibility complex (MHC) class I molecule that is expressed only in the human placenta,
suggesting that it plays an important role at the fetal-maternal interface. In rhesus monkeys, which have similar placentation
to humans, the HLA-G orthologue is a pseudogene. However, rhesus monkeys express a novel placental MHC class I molecule, Mamu-AG, which has HLA-G-like
characteristics. Phylogenetic analysis of AG alleles in two Old World primate species, the baboon and the rhesus macaque, revealed limited diversity characteristic of
a nonclassical MHC class I locus. Gene trees constructed using classical and nonclassical primate MHC class I alleles demonstrated
that the AG locus was most closely related to the classical A locus. Interestingly, gene tree analyses suggested that the AG alleles were most closely related to a subset of A alleles which are the products of an ancestral interlocus recombination event between the A and B loci. Calculation of the rates of synonymous and nonsynonymous substitution at the AG locus revealed that positive selection was not acting on the codons encoding the peptide binding region. In exon 4, however,
the rate of nonsynonymous substitution was significantly lower than the rate of synonymous substitution, suggesting that negative
selection was acting on these codons.
Received: 22 April 1998 / Revised: 15 July 1998 相似文献
19.
Isolation and diversity analysis of resistance gene analogues (RGAs) from cultivated and wild strawberries 总被引:7,自引:0,他引:7
Martínez Zamora MG Castagnaro AP Díaz Ricci JC 《Molecular genetics and genomics : MGG》2004,272(4):480-487
Degenerate oligonucleotide primers, designed based on conserved regions of Nucleotide Binding Site (NBS) domains from previously cloned plant resistance genes, were used to isolate Resistance Gene Analogues (RGAs) from wild and cultivated strawberries. Seven distinct families of RGAs of the NBS-LRR type were identified from two related wild species, Fragaria vesca and F. chiloensis, and six different Fragaria × ananassa cultivars. With one exception (GAV-3), the deduced amino acid sequences of strawberry RGAs showed strong similarity to TIR (Toll Interleukin I Receptor)-type R genes from Arabidopsis, tobacco and flax, suggesting the existence of common ancestors. GAV-3 seemed to be more closely related to the non-TIR type. Further studies showed that the recombination level and the ratio of non-synonymous to synonymous substitutions within families were low. These data suggest that NBS-encoding sequences of RGAs in strawberry are subject to a gradual accumulation of mutations leading to purifying selection, rather than to a diversifying process. The present paper is the first report on RGAs in strawberry.Communicated by M.-A. Grandbastien 相似文献
20.
Plastid sequences of the atpB-rbcL spacer and rbcL gene itself were used to evaluate their respective potential in reconstructing the phylogeny of 15 taxa from the tribe Rubieae (Rubiaceae). From our previous analyses using the atpB-rbcL spacer, the 15 selected taxa represent most of the variability of the tribe. Since this group is considered to be relatively recent (Upper Tertiary), it should allow the study of early dynamics of nucleotide substitutions in recent divergences. The results show that the spacer and rbcL inferred phylogenies are not totally congruent; the spacer trees are more similar to interpretations of morphological data. A comparative analysis of the pattern of nucleotide substitution of these two sequences in the Rubieae shows that (1) the overall rate of substitution is similar in the spacer and in rbcL, and the rate of synonymous substitution in rbcL is much higher; (2) the level of homoplasy is higher in rbcL than in the spacer matrix which shows a higher phylogenetic structure; and (3) the pattern of transition and transversion substitutions is different in the two sequences, and is not linear in rbcL. As a result of these observations, we suggest that (1) the spacer is evolving relatively slowly because of unsuspected, and phylogenetically important; selective constraints on its sequence; and (2) in the rbcL sequence, many sites, free of constraint, are changing at high rate, and some of these sites seem to have undergone multiple substitutions even in this recent tribe. This could explain the high level of homoplasy found in Rubieae rbcL sequences.
Correspondence to: J.-F. Manen 相似文献