共查询到20条相似文献,搜索用时 31 毫秒
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Coetzee Bernard W.T. Ferriera Sam M. Smit Izak P.J. 《Biodiversity and Conservation》2022,31(11):2893-2898
Biodiversity and Conservation - The concept of the “wilderness ethic” is at an impasse. Despite calls for action to conserve wilderness, any notion of wilderness thinking still resides... 相似文献
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Structured regions of α-synuclein fibrils include the early-onset Parkinson's disease mutation sites
Comellas G Lemkau LR Nieuwkoop AJ Kloepper KD Ladror DT Ebisu R Woods WS Lipton AS George JM Rienstra CM 《Journal of molecular biology》2011,411(4):881-895
α-Synuclein (AS) fibrils are the major component of Lewy bodies, the pathological hallmark of Parkinson's disease (PD). Here, we use results from an extensive investigation employing solid-state NMR to present a detailed structural characterization and conformational dynamics quantification of full-length AS fibrils. Our results show that the core extends with a repeated structural motif. This result disagrees with the previously proposed fold of AS fibrils obtained with limited solid-state NMR data. Additionally, our results demonstrate that the three single point mutations associated with early-onset PD—A30P, E46K and A53T—are located in structured regions. We find that E46K and A53T mutations, located in rigid β-strands of the wild-type fibrils, are associated with major and minor structural perturbations, respectively. 相似文献
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《Gene Analysis Techniques》1989,6(1):21-23
A method is presented that facilitates the identification of cDNA clones corresponding to the polyadenylated 3′ end of mRNA. It is based on the use of a poly dT probe that is synthesized by homopolymer extension of commercially available oligo dT. The method is shown to work in Southern blot analysis of plasmid preparations and in situ with colonies. 相似文献
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Conventional wisdom suggests that halting or reversing long-term atherosclerosis requires increasing the amount of circulating high-density lipoprotein (HDL) coursing through the vasculature. However, recent evidence seems to indicate that reducing the size of cholesterol-containing lipid plaques might be accomplished by methods that either do not raise or, in fact, lower the amount of circulating HDL. Carriers of apoAI(Milano), a variant of apoprotein AI (a component of HDL), have reduced levels of circulating low-density lipoprotein (LDL). Intriguingly, these individuals have reduced amounts of circulating HDL and total apoAI. Infusions of the ApoAI(Milano) variant given to patients with coronary atherosclerosis appear to lead to disease regression and reduced plaque size. However, larger studies are required to provide definitive proof of apoAI(Milano)'s benefits. What is certain is that attention should be focused on the removal of cholesterol from plaques rather than simply desiring to raise HDL concentrations in patients. 相似文献
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The life cycle of the red alga Porphyra purpurea alternates between two morphologically distinct phases: a shell-boring, filamentous sporophyte and a free-living, foliose gametophyte. From a subtracted cDNA library enriched for sporophyte-specific sequences, we isolated a cDNA encoding an unusual elongation factor 1 (EF-1) that is expressed only in the sporophyte. A second EF-1 gene that is expressed equally in the sporophyte and the gametophyte was isolated from a genomic library. These are the only EF-1 genes detectable in P. purpurea. The constitutively expressed gene encodes and EF-1 very similar to those of most eukaryotes. However, the sporophyte-specific EF-1 is one of the most divergent yet described, with nine insertions or deletions ranging in size from 1 to 26 amino acids. This is the first report of a developmental stage-specific EF-1 outside of the animal kingdom and suggests a fundamental role for EF-1 in the developmental process. 相似文献
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Interactions between insects and their baculovirus pathogens are often described using simple disease models. Baculoviruses, however, are transmitted when insects consume virus-contaminated foliage, and foliage variability, whether within or between host-plant species, can affect viral infectiousness. Insect-baculovirus interactions may thus be embedded in a tritrophic interaction with the insect's host plant, but disease models include only the host and the pathogen. We tested these models by measuring the transmission of a baculovirus of gypsy moths (Lymantria dispar) on red oak (Quercus rubra) and white oak (Quercus alba) in the field in six experiments over four years. In all experiments, there were only weak effects of host-tree species, and in only one did the best-fitting model include tree species effects. These weak effects of foliage variability on transmission were not due to a lack of foliage variability on viral infectiousness, because when larvae were force-fed virus-contaminated foliage, infection rates were higher on white oak. Our results suggest that feeding behavior plays an important role in baculovirus transmission and that models can usefully describe baculovirus dynamics even without including foliage variability. Our work provides a clear example of how two-species models are sometimes sufficient to describe what appear to be tritrophic interactions. 相似文献
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Pedro Monterroso Neftalí Sillero Luís Miguel Rosalino Filipa Loureiro Paulo Célio Alves 《Ecology and evolution》2013,3(7):2285-2295
Most studies dealing with home ranges consider the study areas as if they were totally flat, working only in two dimensions, when in reality they are irregular surfaces displayed in three dimensions. By disregarding the third dimension (i.e., topography), the size of home ranges underestimates the surface actually occupied by the animal, potentially leading to misinterpretations of the animals' ecological needs. We explored the influence of considering the third dimension in the estimation of home‐range size by modeling the variation between the planimetric and topographic estimates at several spatial scales. Our results revealed that planimetric approaches underestimate home‐range size estimations, which range from nearly zero up to 22%. The difference between planimetric and topographic estimates of home‐ranges sizes produced highly robust models using the average slope as the sole independent factor. Moreover, our models suggest that planimetric estimates in areas with an average slope of 16.3° (±0.4) or more will incur in errors ≥5%. Alternatively, the altitudinal range can be used as an indicator of the need to include topography in home‐range estimates. Our results confirmed that home‐range estimates could be significantly biased when topography is disregarded. We suggest that study areas where home‐range studies will be performed should firstly be scoped for its altitudinal range, which can serve as an indicator for the need for posterior use of average slope values to model the surface area used and/or available for the studied animals. 相似文献
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Kovats RS Campbell-Lendrum DH McMichael AJ Woodward A Cox JS 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2001,356(1411):1057-1068
The world's climate appears now to be changing at an unprecedented rate. Shifts in the distribution and behaviour of insect and bird species indicate that biological systems are already responding to this change. It is well established that climate is an important determinant of the spatial and temporal distribution of vectors and pathogens. In theory, a change in climate would be expected to cause changes in the geographical range, seasonality (intra-annual variability), and in the incidence rate (with or without changes in geographical or seasonal patterns). The detection and then attribution of such changes to climate change is an emerging task for scientists. We discuss the evidence required to attribute changes in disease and vectors to the early effects of anthropogenic climate change. The literature to date indicates that there is a lack of strong evidence of the impact of climate change on vector-borne diseases (i.e. malaria, dengue, leishmaniasis, tick-borne diseases). New approaches to monitoring, such as frequent and long-term sampling along transects to monitor the full latitudinal and altitudinal range of specific vector species, are necessary in order to provide convincing direct evidence of climate change effects. There is a need to reassess the appropriate levels of evidence, including dealing with the uncertainties attached to detecting the health impacts of global change. 相似文献
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Roswitha Wiltschko Wolfgang Wiltschko 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1985,157(4):469-476
Summary To find out whether the initial orientation of pigeons is affected by a spontaneous directional tendency, as postulated by Wallraff's (1974, 1978, 1982) hypothesis, experienced birds were released at test sites distributed symmetrically around their loft. — The length of the mean vectors of the single releases, the deviations from the home direction, the homeward components as well as the homing speed did not show a correlation with the geographic position of the home direction. Summarizing four sites each on 21 experimental circles, we frequently obtained significant compass vectors, but they varied in direction between 115° ESE and 351° N, depending on what sites had been used, and did not indicate a uniform trend.A preferred compass direction as an integrated part of pigeon navigation, being the reason for the frequently observed deviations from the home direction, could not be confirmed. — The problematic nature of simply pooling the data of several symmetrically distributed test sites and calling any resulting significant vector a preferred compass direction is discussed, together with other possible reasons for asymmetrical distributions of release site biases.Abbreviation
PCD
preferred compass direction 相似文献
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Megumi Igarashi Yoshie Abe Yoshimichi Hatsuyama Takanori Ueda Tomoko Fukasawa-Akada Tomoyuki Kon Tsuyoshi Kudo Takashi Sato Masahiko Suzuki 《Molecular breeding : new strategies in plant improvement》2008,22(1):95-118
Two apple genetic linkage maps were constructed using amplified fragment length polymorphisms (AFLPs), simple sequence repeats
(SSRs), random amplified polymorphic DNAs (RAPDs), and expressed sequence tag (EST)-derived markers in combination with a
pseudo-testcross mapping strategy in which the cultivars ‘Ralls Janet’ and ‘Delicious’ were used as the respective seed parents.
Mitsubakaido (Malus sieboldii) was used as the pollen parent for each of the segregating F1 populations. Expressed sequence tag data were obtained from the random sequencing of cDNA libraries constructed from in vitro
cultured shoots and maturing fruits of cv ‘Fuji’, which is the offspring of a cross between ‘Ralls Janet’ and ‘Delicious’.
In addition, a number of published gene sequences were used to develop markers for mapping. The ‘Ralls Janet’ map consisted
of 346 markers (178 AFLPs, 95 RAPDs, 54 SSRs, 18 ESTs, and the S locus) in 17 linkage groups, with a total length of 1082 cM, while that of ‘Delicious’ comprised 300 markers (120 AFLPs,
81 RAPDs, 64 SSRs, 32 ESTs, and the S, Rf, and MdACS-1 loci) on 17 linkage groups spanning 1031 cM. These maps are amenable to comparisons with previously published maps of ‘Fiesta’
and ‘Discovery’ (Liebhard et al., Mol Breed 10:217–241, 2002; Liebhard et al., Theor Appl Genet 106:1497–1508, 2003a) because several of the SSRs (one to three markers per linkage group) were used in all of the maps. Distorted marker segregation
was observed in three and two regions of the ‘Ralls Janet’ and ‘Delicious’ maps, respectively. These regions were localized
in different parts of the genome from those in previously reported apple linkage maps. This marker distortion may be dependent
on the combinations of cultivars used for map construction. 相似文献
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Three methods of estimating photorespiratory rate in leaves of the C3–C4 intermediate species Moricandia arvensis and the related C3 species Moricandia moricandioides were compared. The results indicated that the photorespiratory rate in M. arvensis is less than in M. moricandioides, and that this is caused partly by reduced carbon flux through the photorespiratory pathway, and partly by the presence of a mechanism for enhanced photorespiratory CO2 reassimilation in the intermediate species. Measurements of the CO2 compensation point () in the two species supported this conclusion. A functional C4 pathway is unlikely to be involved in the reduction of photorespiratory rate in M. arvensis since pulse-chase experiments showed that carbon did not move from C4 acids to the reductive pentose-phosphate pathway in attached leaves under steady-state conditions at .Abbreviations and symbols APR
apparent photosynthetic rate
- Ci, Ce
intercellular, external CO2 concentration
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CO2 compensation point
- PAR
photosynthetically active radiation
- PFD
photon flux density 相似文献
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Diplozoidae and Octomacridae are usually considered as sister families. Essentially this is because they are the only polyopisthocotyleans parasitising primary freshwater teleosts. Because of the lack of phylogenetically informative morphological characters to explore the pattern of colonisation of the primary continental freshwater teleosts and in order to understand the appearance of the "natural parabiosis" of Diplozoidae, a molecular phylogeny was inferred by comparing newly obtained partial 28S and 18S rDNA gene sequences of Eudiplozoon nipponicum and Diplozoon homoion with other already available sequences. The phylogenetic analysis seems to show that Diplozoidae and Octomacridae are not sister groups. Thus, the colonisation of primary freshwater teleosts by these two families could be independent. 相似文献
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Ronald E. Martin Thomas Servais 《Lethaia: An International Journal of Palaeontology and Stratigraphy》2020,53(1):5-31
We discuss the possible links between the fossil record of marine biodiversity, nutrient availability and primary productivity. The parallelism of the fossil records of marine phytoplankton and faunal biodiversity implicates the quantity (primary productivity) and quality (stoichiometry) of phytoplankton as being critical to the diversification of the marine biosphere through the Phanerozoic. The relatively subdued marine biodiversity of the Palaeozoic corresponds to a time of relatively low macronutrient availability and poor food quality of the phytoplankton as opposed to the diversification of the Modern Fauna through the Mesozoic–Cenozoic. Increasing nutrient runoff to the oceans through the Phanerozoic resulted from orogeny, the emplacement of Large Igneous Provinces (LIPs), the evolution of deep-rooting forests and the appearance of more easily decomposable terrestrial organic matter that enhanced weathering. Positive feedback by bioturbation of an expanding benthos played a critical role in evolving biogeochemical cycles by linking the oxidation of dead organic matter and the recycling of nutrients back to the water column where they could be re-utilized. We assess our conclusions against a recently published biogeochemical model for geological time-scales. Major peaks of marine diversity often occur near rising or peak fluxes of silica, phosphorus and dissolved reactive oceanic phosphorus; either major or minor 87Sr/86Sr peaks; and frequently in the vicinity of major (Circum-Atlantic Magmatic Province) and minor volcanic events, some of which are associated with Oceanic Anoxic Events. These processes appear to be scale-dependent in that they lie on a continuum between biodiversification on macroevolutionary scales of geological time and mass extinction. 相似文献
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L. Lindstr?m R. V. Alatalo J. Mappes 《Proceedings. Biological sciences / The Royal Society》1997,264(1379):149-153
Batesian mimicry is the resemblance between unpalatable models and palatable mimics. The widely accepted idea is that the frequency and the unprofitability of the model are crucial for the introduction of a Batesian mimic into the prey population. However, experimental evidence is limited and furthermore, previous studies have considered mainly perfect mimicry (automimicry). We investigated imperfect Batesian mimicry by varying the frequency of an aposematic model at two levels of distastefulness. The predator encountered prey in a random order, one prey item at a time. The prey were thus presented realistically in a sequential way. Great tits (Parus major) were used as predators. This experiment, with a novel signal, supports the idea that Batesian mimics gain most when the models outnumber them. The mortalities of the mimics as well as the models were significantly dependent on the frequency of the model. Both prey types survived better the fewer mimics there were confusing the predator. There were also indications that the degree of distastefulness of the model had an effect on the survival of the Batesian mimic: the models survived significantly better the more distasteful they were. The experiment supports the most classical predictions in the theories of the origin and maintenance of Batesian mimicry. 相似文献
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