Effects of oxidoreduction potential combined with acetic acid,NaCl and temperature on the growth,acidification, and membrane properties of Lactobacillus plantarum

Antibody phage display libraries (Griffin and Tomlinson I) displaying antibody genes and maintained and amplified in Escherichia coli were used to isolate antibodies to the hapten target microcystin LR (1000 Da) conjugated to either bovine serum albumin or keyhole limpet haemocyanin. In competition enzyme-linked immunosorbent assay, bacterially expressed antibodies selected via the Griffin library showed at least 300 times greater sensitivity than those isolated from the Tomlinson library, for free microcystin. Bacterially expressed phage antibody libraries provide a rapid and relatively easy route for the selection of monoclonal antibodies specific for even the most difficult of antigenic targets such as free haptens.     

Oppositions in panbiogeography: Can the conflicts between selection,constraint, ecology,and history be resolved?

Abstract

Croizat’s radical attack on traditional modes of biological inquiry has produced strongly polarised responses. Instead of arguing for or against the panbiogeographic approach I attempt to analyse some basic assumptions shared by both its defenders and critics. Although their emphasis is quite different both sides rely on two central oppositions. In evolutionary arguments selective explanations are opposed to those emphasising phylogenetic constraint (orthogenesis). In biogeographic arguments ecological explanations are opposed to historical explanations. I discuss how the legendary opposition between nature and nurture was resolved by reformulating the way in which causation was viewed. I suggest that the selection/constraint opposition shares many features in common with the nature/nurture opposition and argue that it can be resolved in a similar manner. Conflicts between ecological and historical explanations in biogeography are explored by contrasting Diamond’s analysis of New Guinea bird distributions with that of Croizat’s. By again drawing on the resolution of the nature/nurture dispute a way of synthesising ecological and historical explanations is outlined.     

Other cities,same problems.

London''s health care problems, particularly underfunding, are mirrored in other major cities in the United Kingdom and abroad. None has found the perfect solution, but the debates on the Tomlinson report provide an opportunity to review how to manage the three major areas of service provision, medical education, and research in London. Mr Robert Maxwell suggests that some aspects of the successful ambulatory care initiative in New York could be adapted by the capital''s primary care providers while the secondary services might learn from the rationalisation of specialist services in Paris.     

The Social Life of Death on Delhi's Streets: Unclaimed Souls,Pollutive Bodies,Dead Kin and the Kinless Dead

ABSTRACT

How does the status of ‘street children’ in life inflect the narrative representation of their deaths? Street-dwelling children's interactions with death in North India reveal much about how their identities are produced in public domains. In this paper, I examine several instances of ‘homeless child’ death to illuminate the place of such subjects in society and urban space, and to interrogate the degree to which they can be rendered ‘recognizable’ or ‘grievable’, in Butler's (2010) terminology. In particular, I explore the presence or absence of kin in the ways that child death is narrated. I also explore the related question of how living ‘vagabond (aawara) children’ situate their status in narratives of death and loss. I conclude with discussions of how children negotiate their orientations towards death through ghost narratives, and of the space-, economy- and age-bound assignment of pollutive tasks once reserved for low castes to street-dwelling children.     

Strangers no more’s several contributions (and my few regrets)

ABSTRACT

The main strengths of the book include, I argue, the authors’ sophisticated conceptualization of immigrants’ and their offspring’s integration into the host society as complex, multi-dimensional processes which can contain contradictory tendencies; a well-executed and informative comparative analysis of these developments in several countries with – rare among the social scientists – a firm anchoring of the present situations in the past from which they emerge; and useful practical suggestions for policy-making derived by the authors from their findings. Rather than outright criticisms, I then present a few regrets regarding, on the one hand, the book’s presentation style or what I felt were some too forcefully stated claims, and, on the other hand, some un(der)explored issues which, in my opinion, merited more analytic attention.     

Musicality in human evolution,archaeology and ethnography

This essay reviews Iain Morley’s The Prehistory of Music, an up-to-date and authoritative overview of recent research on evolution and cognition of musicality from an interdisciplinary viewpoint. Given the diversity of the project explored, integration of evidence from multiple fields is particularly pressing, required for any novel evolutionary account to be persuasive, and for the project’s continued progress. Moreover, Morley convincingly demonstrates that there is much more to understanding musicality than is supposed by some theorists. I outline Morley’s review of the archaeological and ethnographic literature, and then go on to critique his assessment of philosophical and evolutionary theories, offering some alternative perspectives that might better benefit his project.     

Elusive but not hypothetical: axillary meristems in Wollemia nobilis

BACKGROUND: The branches of Wollemia nobilis are unbranched; however, it has been noted that new branches can form from the distal end of damaged ones, and branches can grow from axillary structures once a terminal strobilus has fallen. Tomlinson and Huggett (2011, Annals of Botany 107: 909-916) have recently investigated the formation of these reiterative branches and stated in the title of their paper that 'Partial shoot reiteration in Wollemia nobilis (Araucariaceae) does not arise from "axillary meristems"'. They go on to state 'Further research may reveal the presence of these elusive, but still only hypothetical, axillary meristems'. RESPONSE: In this Viewpoint, I argue that Tomlinson and Huggett do not refer to previously published information that indicates that axillary meristems are present in Wollemia nobilis branch leaf axils, and that their anatomical methods were probably not optimal for locating and examining these minute structures. Thus, whilst I would agree that the axillary meristems in branch leaf axils of Wollemia nobilis are elusive, I contend that they are not hypothetical.     

Complexity,adaptive complexity and the Creative View of natural selection

In this paper, I respond to arguments proposed by Brunnander in this journal issue concerning my position regarding the Creative View of natural selection (Razeto-Barry & Frick, 2011). Brunnander argues that (i) the Creative View we defend does not serve to answer William Paley’s question because (ii) Paley’s question is “why there are complex things rather than simple ones” and (iii) natural selection cannot answer this question. Brunnander’s arguments for (iii) defend a Non-creative View of natural selection (sensu Razeto-Barry & Frick, 2011). Here I claim that Brunnander’s arguments for (iii) are mistaken and I also argue that even accepting (iii) we do not have to accept (i), given that statement (ii) is historically and conceptually flawed. Thus here I analyze Paley’s question from a historical point of view and from a contemporary perspective in a quest for the potential conceptual relevance of Paley’s question today. In this vein I argue that from a contemporary point of view statement (iii) may be correct but for different reasons than those adduced by Brunnander.     

Environmental Values,Human Nature,and Economic Democracy

Recent social science indicates that the public at large behave more ethically, and favor environmental protection more strongly, than do the wealthiest minority. Yet the latter group exerts predominant control over the economy. This suggests that shifting power away from this minority and onto the majority would yield a better ecology. In this paper I spell out the implications of these considerations for “economic democracy” (ED), a well-developed alternative to capitalism that shifts power from wealthy shareholders onto ordinary citizens and workers. I contrast this rationale for ED with some thinkers’ defense of “sustainable capitalism”, and with others’ ecological arguments for ED based on economic stability and self-interest, rather than ethical behavior per se.     

Whose crisis counts? Intersectionality,austerity and the politics of survival

Foregrounding the voices of minority women in England, Scotland and France, Minority Women and Austerity provides a rich and compelling account of austerity’s lived effects. Through its intersectional analyses and historically attendant frame, the book locates minority women’s experiences of austerity not as fundamentally “new” but rather as an extension and sharpening of conditions of “routinized crisis” and inequality. In doing so it compels its readers to ask: whose crisis counts? In this review paper, I summarize the book’s key contributions. In particular, I discuss the book’s theorizations of “political racelessness” to critique the erasure of minority women’s experiences and activism. I reflect on what this means for those researching austerity and its deleterious effects, and for those participating in anti-austerity activism. I end by discussing the analytical tools that the book offers for interrogating the Left’s response to on-going austerity within a post-Brexit Britain.     

Synthetic Biology and the Moral Significance of Artificial Life: A Reply to Douglas,Powell and Savulescu

I discuss the moral significance of artificial life within synthetic biology via a discussion of Douglas, Powell and Savulescu's paper 'Is the creation of artificial life morally significant’. I argue that the definitions of 'artificial life’ and of 'moral significance’ are too narrow. Douglas, Powell and Savulescu's definition of artificial life does not capture all core projects of synthetic biology or the ethical concerns that have been voiced, and their definition of moral significance fails to take into account the possibility that creating artificial life is conditionally acceptable. Finally, I show how several important objections to synthetic biology are plausibly understood as arguing that creating artificial life in a wide sense is only conditionally acceptable.     

Police and Thieves,Gunmen and Drunks: Problems with Men and Problems with Society in Papua New Guinea

The image of the ‘man with a gun’ is pervasive in Papua New Guinea and connotes not only the state's capacity to use force, but that of men to resist and subvert state control. At the same time, the association of beer and marijuana with both modernity and violent masculine behaviours provides the context, the justification and the forms of homosocial activities involving violence. In this paper, I explore the ambiguities surrounding guns as instruments of state force and as symbols of masculine autonomy in so‐called ‘weak states’ by examining some stories about the ways that guns are acquired for illegal activities. In particular, I shall discuss the ways that guns and beer are instruments of violence and potency for police, tribal warriors and criminals as well as some of the means whereby men gain access to new forms of power. Drawing on ethnographic research with young men in New Ireland Province, the paper will deal specifically with the ways that adolescent boys construe ‘modern masculinity’.     

Charles Darwin,embryology, evolution and skeletal plasticity

Darwin provided us with the theory of evolutionary change through natural selection. Just as important to the science of biology was Darwin’s recognition that all organisms could be classified and were related to one another because they arose from a single common universal ancestor – what we know as the universal tree of life (UtoL). All the features of the skeletal biology of fish therefore can be explained, both in an evolutionary framework (ultimate causation) and in the framework of development, growth and physiology (proximate causation). Neither approach is complete without the other. I will outline the elements of Darwin’s theories on evolution and classification and, as importantly, discuss what was missing from Darwin’s theories. An important class of evidence for evolution used by Darwin came from embryology, both comparative embryology and the existence of vestiges and atavisms. After discussing this evidence I examine some fundamental features of skeletal development and evolution These include: the presence of four skeletal systems in all vertebrates; the existence of two skeletons, one based on cartilage, the other on bone and dentine; the modular nature of skeletal development and evolution; and the plasticity of the skeleton in response to either genetic or environmental changes.     

Troubled landscapes,troubling anthropology: co‐presence,necessity, and the making of ethnographic knowledge

If ‘co‐presence is a condition of [anthropological] inquiry’ (Fabian), what sort of knowledge does it produce? I explore this question through an ethnography of a ‘troubled landscape’ in Malaysian Borneo: a lush, hilly region that has been the site of a dam construction and resettlement project since the late 2000s. My article uses the notion of co‐presence as both a lens through which to explore the predicaments of the four small communities affected by the scheme and a reflexive device that underscores the embeddedness of the ethnographic encounter in a larger relational field – one characterized as much by chance and necessity as it is by anthropologists’ intellectual agendas. In the process, I seek to trouble some of the methodological and ethical issues posed by anthropology's recent ‘ontological turn’, notably the long‐standing questions of what it means to ‘take seriously’ and how ethnography and the ethnographer are implicated in this project.     

Possibility,legitimacy, and the new ontologies of choice: a comment on Brubaker

In Trans, Rogers Brubaker makes a major argument about the contentious politics of the contemporary self. In this commentary, I first lay out what is think is the solidity of the book’s contrasting tableau of the functioning of race and gender in American society and beyond. I then point to Brubaker’s bundling together of issues of legitimacy and issues of ontology and begin to imagine what alternative analyses can come out of their unbundling – suggesting that race and gender are perhaps more analogous social formations than Trans argues. Finally, I bring attention to the role of ontological hierarchy in the formulation and policing of identity claims and conclude that the return of biology and the new empire of choice may not be two parallel, independent developments but one and the same process. Theoretically, I amplify one of the book’s epistemological contributions by calling for a reflexive turn in social-constructionist thought.     

Redefining ecological engineering to promote its integration with sustainable development and tighten its links with the whole of ecology

Ecological engineering was defined several decades ago, both in the academic field and in management. However, ecological engineering seems to be re-emerging as an academic field and as a cornerstone concept in French ecologists’ writings. I first summarize Barbault and Pavé's [Barbault, R., Pavé, A., 2003. Territoire de l’écologie et écologie des territoires. In: Caseau, P. (Ed.), Etudes sur l’environnement: de l’échelle du territoire à celle du continent, Tec et Doc Lavoisier, Paris, pp. 1–49] point of view on why ecological engineering now seems rehabilitated in France. I next propose a definition of ecological engineering, in accordance with the two reasons for its French re-emergence, i.e. the prevalence of the concept of sustainable development and the development of applied ecological sub-disciplines. This leads us to suggest that ecological engineering should be ecological in the broad sense, and not only targeted to the ecosystem level. I end the paper by discussing some problems and characteristics of ecological engineering that stem from this definition.     

Stability,sensitivity and uncertainty rates in the flow equations of ecological models

In previous work by the authors about dynamic system modeling, basic ecosystems concepts and their application to ecological modeling theory were formalized. Measuring how a variable effects certain processes leads to improvements in dynamic systems modelling and facilitates the author’s study of system diversity in which model sensitivity is a key theme. Initially, some variables and their numeric data are used for modeling. Predictions from the constructed models depend on these data. Generic study of sensitivity aims to show to what degree model behavior is altered by modification of some specific data. If small variations cause important modifications in the model’s global behavior then the model is very sensitive in relation to the variables used. If uncertain systems are considered, then it is important to submit the system to extreme situations and analyze its behavior. In this article, some indexes of uncertainty will be defined in order to determine the variables' influence in the case of extreme changes. This will permit analysis of the system’s sensitivity in relation to several simulations.     

Shared secrecy: teasing,attachment, and children's emotional management in rural southern Mongolia

In recent decades, anthropologists have scrutinized psychologists’ claim that humans process information about others by imputing mental states. The debate remains open whether cross-cultural variability in how people conceive of minds and inner states reflects differences in their folk theories only, or whether it engenders deep-grained psychological differences. In this article, I look at the very onset of children's apprenticeship in emotional management to examine its cognitive consequences. In the context of rural Mongolia in the late 2000s, questions about homesickness prompted by adults raised children's awareness of the existence of a private self and by the very same process discouraged verbalization or public display of personal feelings. I start by presenting interactions where children were routinely teased about homesickness, and invited to deny that they missed their family. I contextualize this teasing routine within local conceptions about emotional control and linguistic ideology to unveil how it introduced children to the performative power of words while also making them face moral dilemmas. The irreconcilable tension between behaving according to expectations of self-control and experiencing separation induced children's discovery of the possibility of disconnecting intimate feelings from public self-presentation, thus transforming the experience of separation into an experience of secret connection. I speculate that the secrecy that hallows homesickness contributed to making it all the more poignant. The way adults constantly checked on whether children were missing their home prompted children to actually identify some emotional state or aspect of their experience as ‘missing home’. It gave a name to a personal experience which thus became social and guaranteed that it became a shared feeling, albeit a secret one.     

The state as a whiteman,the whiteman as a /’hun: personhood,recognition, and the politics of knowability in the Kalahari

The article is dedicated to the loving memory of !A|’xuni.

The Ju|’hoansi of east central Namibia sometimes refer to the state as a whiteman and to the whiteman as a /’hun (steenbok). In this article, I contextualize these naming practices by tracing the history of colonial encounters on the fringes of the Western Kalahari through a small-scale animist perspective. I then discuss what this means for the concept of ‘recognition’, which I treat as a two-way intersubjective process of making oneself un/knowable to others. I argue that the Ju|’hoansi have engaged in parallel processes of mis/recognition vis-à-vis their colonial Others. By failing to enter into reciprocal relations with the Ju|’hoansi, the whiteman and the state have remained outside of the Ju|’hoansi's social universe and have thus compromised their own personhood.     

Life, death, and humor: approaches to storytelling in Native America

Edith Turner has been studying healing as a sensitive, spiritually attuned participant-observer for a long time. Despite her academic background, experiential learning and knowing are important parts of Turner’s approach to research. Her efforts to understand healing have taken her on journeys to Africa, Mexico, Ireland, and more recently, Alaska’s North Slope. In these contexts, she has experienced healing offered by others, and learned to heal in various traditional ways herself. In her book, The Hands Feel It (1996), Turner focuses on the role that touch and spirit presence have in healing in a North Slope I?upiat community. However, her book makes clear that narrative and storytelling are important parts of the healing process, as well. In this paper, Turner elaborates on some aspects of the connection between narrative and healing based on her North Slope experience.