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Antonini JM Leonard SS Roberts JR Solano-Lopez C Young SH Shi X Taylor MD 《Molecular and cellular biochemistry》2005,279(1-2):17-23
Questions exist concerning the potential carcinogenic effects after welding fume exposure. Welding processes that use stainless
steel (SS) materials can produce fumes that may contain metals (e.g., Cr, Ni) known to be carcinogenic to humans. The objective
was to determine the effect of in vitro and in vivo welding fume treatment on free radical generation, DNA damage, cytotoxicity and apoptosis induction, all factors possibly
involved with the pathogenesis of lung cancer. SS welding fume was collected during manual metal arc welding (MMA). Elemental
analysis indicated that the MMA-SS sample was highly soluble in water, and a majority (87%) of the soluble metal was Cr. Using
electron spin resonance (ESR), the SS welding fume had the ability to produce the biologically reactive hydroxyl radical (•OH), likely as a result of the reduction of Cr(VI) to Cr(V). In vitro treatment with the MMA-SS sample caused a concentration-dependent increase in DNA damage and lung macrophage death. In addition,
a time-dependent increase in the number of apoptotic cells in lung tissue was observed after in vivo treatment with the welding fume. In summary, a soluble MMA-SS welding fume was found to generate reactive oxygen species
and cause DNA damage, lung macrophage cytotoxicity and in vivo lung cell apoptosis. These responses have been shown to be involved in various toxicological and carcinogenic processes.
The effects observed appear to be related to the soluble component of the MMA-SS sample that is predominately Cr. A more comprehensive
in vivo animal study is ongoing in the laboratory that is continuing these experiments to try to elucidate the potential mechanisms
that may be involved with welding fume-induced lung disease. 相似文献