共查询到20条相似文献,搜索用时 15 毫秒
1.
F. Ueda K. B. Raja R. J. Simpson I. S. Trowbridge† M. W. B. Bradbury 《Journal of neurochemistry》1993,60(1):106-113
Abstract: Uptake of 59 Fe from blood into brains of anaesthetized rats and mice has been studied by intravenous infusion of [59 Fe]ferrous ascorbate or of 59 Fe-transferrin, the results not being significantly different. Uptakes in the rat were linear with time, but increased at longer times in the mouse. Transfer constants, K in (in ml/g/h × 103 ), for cerebral hemispheres were 5.2 in the adult rat and 5.6 in the mouse. These K in values corresponded to 59 Fe influxes of 145 and 322 pmol/g/h, respectively. 59 Fe uptake into the mouse brain occurred in the following order: cerebellum > brainstem > frontal cerebral cortex > parietal cortex > occipital cortex > hippocampus > caudate nucleus. In genetically hypotransferrinaemic mice, 59 Fe uptake into brain was 80–95 times greater than in To strain mice. Pretreatment of young rats and mice with monoclonal antibodies to transferrin receptors, i.e., the anti-rat immunoglobulin G OX 26 and the anti-mouse immunoglobulin M RI7 208, inhibited 59 Fe uptake into spleen by 94% and 98%, respectively, indicating saturation of receptors. The antibodies reduced 59 Fe uptake into rat brain by 35–60% and that into mouse brain by 65–85%. Although a major portion of iron transport across the blood-brain barrier is normally transferrin-mediated, non-transferrin-bound iron readily crosses it at low serum transferrin levels. 相似文献
2.
Abstracts. Parameters of recognizable erythroid cell proliferation were measured in four groups of normal rats weighing 50, 100, 150 and 300 g in order to provide a comprehensive set of data suitable for a re-investigation of erythropoietic models. Total erythroblast cellularity was measured by the 59 Fe technique, DNA synthesis time by quantitative 14 C-autoradiography, and the erythrocyte production rate was derived from the increase with time of the erythrocyte labelling index after repeated injections of 3 H-leucine. Furthermore, the relative erythroblast density was determined in the various morphological compartments. From the total erythroid cell mass in DNA synthesis and the absolute erythrocyte production rate, figures were derived for the mean DNA synthesis time of erythroid cells and compared with the directly measured ones. The discrepancies in all weight groups between direct and indirect determination of DNA synthesis time were considerable. In a previous study re-evaluation of comparable data in literature had revealed comparable inconsistencies. Since a critical discussion of possible errors in the experimental techniques does not indicate data acquisition to be the principal source of disagreement it is concluded that the type of model applied to describe how cells pass the boundaries of morphological cell compartments is of high significance. Models based on a sequential flux of cells through the individual compartments are inadequate for evaluation of the presented set of data. 相似文献
3.
The granulocytic chalone is secreted by mature granulocytes and inhibits 3 H-thymidine incorporation of proliferating granulocytes in vitro . The effect and the cell line specificity of this chalone was assessed with the in vivo diffusion chamber culture technique. Tests were carried out on cultures from normal mouse bone marrow cells and mouse and rat blood leucocytes. The majority of the DNA synthesizing cells in marrow cultures were proliferating granulocytes. Macrophages and immunoblasts proliferated in rat leucocyte cultures, when the chambers had been carried for 5 days in host mice. Repeated chalone or control injections were given i.p. to the host mice during 6–7 hr prior to 3 H-thymidine injection. Isotope uptake of proliferative granulocytes was reduced by the chalone treatment. No such effect was found on the rat immunoblasts and macrophages. The viability of cultured cells was apparently not affected by the chalone treatment. 相似文献
4.
TRACER KINETIC MODEL ANALYSIS OF POTASSIUM UPTAKE BY DISSOCIATED NERVE CELL CULTURES: GLIAL-NEURONAL INTERRELATIONSHIP 总被引:1,自引:1,他引:0
Abstract— Potassium transport in dissociated nerve cell cultures has been studied by analysing 86 Rb and 42 K uptake. For this purpose cultures prepared from cerebral hemispheres of both 8- and 14-day-old chick embryos were used in short-term experiments. The potassium pool is in the steady-state in all the cultures. The 86 Rb is taken up more rapidly that 42 K; however the ratio of amounts of 86 Rb and 42 K incorporated is constant with time and under different conditions. The run of kinetic curves of uptake has been studied by tracer kinetic model analysis. As a result of this analysis a glial-neuronal interrelationship in transport has been suggested. Furthermore, it was shown that not only glial cells but neurons also take up potassium directly from the medium. Comparing the incorporated amounts in different cultures, a decreased uptake can be seen where glial cells prevail. The significance of this fact is discussed. 相似文献
5.
L. E. Feinendegen H. J. Heiniger G. Friedrich E. P. Cronkite † 《Cell proliferation》1973,6(6):573-585
Swiss Albino mice received a single i.v. injection of 3 H-thymidine (TdR) or of 125 I-deoxyuridine (IUdR). Bone marrow, thymus, spleen and mesenteric lymph node were examined for the efficiency of precursor incorporation into DNA, and for DNA renewal from day 1 to day 8.
TdR is 5–8 times more efficiently incorporated by the different organs in vivo and in vitro than is IUdR. This indicates that the discrimination against IUdR occurs at the level of DNA synthesizing cells.
A diminished DNA turnover rate measured with3 H-TdR in comparison with 125 I-UdR is interpreted to indicate reutilization of TdR.
TdR reutilization was observed in bone marrow and spleen from at least day 1 on, and in the thymus from day 3 on, following pulse labeling of DNA synthesizing cells. The degree of TdR reutilization appears higher in the thymus (67%) than the bone marrow (43%) and spleen (38%). The mesenteric lymph node indicates either no, or a very low efficiency of TdR reutilization. The data are also consistent with a reutilization equally efficient for TdR and IUdR.
It is suggested that the TdR salvage pathway in hemopoietic tissues is largely localized to single organs which have immediate access to TdR made available by catabolism of DNA. The contribution of TdR from systemic reutilization to the organs studied falls within the limits of error of measurements. Moreover, the TdR salvage pathway especially in the lymph node may involve other DNA breakdown products than nucleosides. 相似文献
TdR is 5–8 times more efficiently incorporated by the different organs in vivo and in vitro than is IUdR. This indicates that the discrimination against IUdR occurs at the level of DNA synthesizing cells.
A diminished DNA turnover rate measured with
TdR reutilization was observed in bone marrow and spleen from at least day 1 on, and in the thymus from day 3 on, following pulse labeling of DNA synthesizing cells. The degree of TdR reutilization appears higher in the thymus (67%) than the bone marrow (43%) and spleen (38%). The mesenteric lymph node indicates either no, or a very low efficiency of TdR reutilization. The data are also consistent with a reutilization equally efficient for TdR and IUdR.
It is suggested that the TdR salvage pathway in hemopoietic tissues is largely localized to single organs which have immediate access to TdR made available by catabolism of DNA. The contribution of TdR from systemic reutilization to the organs studied falls within the limits of error of measurements. Moreover, the TdR salvage pathway especially in the lymph node may involve other DNA breakdown products than nucleosides. 相似文献
6.
Autoradiography and scintillation counting have been used for evaluation of lymphocyte turnover and life span in the bone marrow, peripheral blood and thoracic duct lymph of BALB/C mice. It was shown that the bone marrow contained two populations of small lymphocytes. One population was labelled 100% after 3–4 days of intensive injections of 3 H-thymidine and constituted about 75% of the lymphocytes. The remaining 25% of the lymphocytes turned over at a much slower rate comparable to the rate of increase in labelled small lymphocytes of the thoracic duct. More than 10% of the small lymphocytes of the bone marrow were found to be unlabelled after 10 days of intensive injections of 3 H-thymidine. Nine weeks after giving 3 H-thymidine for 30 consecutive days, 8·6% of the small lymphocytes in the bone marrow remained labelled. The mean grain counts of cells in this population were comparable to those of thoracic duct lymphocytes at corresponding times. About 90% of the peripheral blood lymphocytes were found to have a slow turnover and a long life span. 相似文献
7.
Prospective isolation and characterization of mesenchymal stem cells from human placenta using a frizzled-9-specific monoclonal antibody 总被引:1,自引:0,他引:1
Battula VL Treml S Abele H Bühring HJ 《Differentiation; research in biological diversity》2008,76(4):326-336
Abstract We have recently shown that frizzled-9 (FZD9, CD349) is expressed on the cell surface of cultured mesenchymal stromal cells (MSC) derived from the human bone marrow (BM) and chorionic placenta (PL). To study whether FZD9 is also a marker for naive mesenchymal stem cells (MSC), we analyzed the expression pattern of FZD9 on freshly isolated PL cells and determined the clonogenic potential of isolated FZD9+ cells using the colony-forming units-fibroblastic (CFU-F) assay. About 0.2% of isolated PL cells were positive for FZD9. Two-color analysis revealed that FZD9+ PL cells uniformly express CD9, CD63, and CD90, but are heterogeneous for CD10, CD13, and CD26 expression. In contrast to BM-derived MSC, PL-derived MSC expressed only low levels of CD271. Colony assays of sorted cells showed that clonogenic CFU-F reside exclusively in the FZD9+ but not in the FZD9− fraction. Further analysis revealed that CFU-F were enriched by 60-fold in the FZD9+ CD10+ CD26+ fraction but were absent in the FZD9+ CD10− CD26− population. Cultured FZD9+ cells expressed the embryonic stem cell makers Oct-4 and nanog as well as SSEA-4 and TRA1-2-49/6E. In addition, they could be differentiated into functional adipocytes and osteoblasts. This report describes for the first time that FZD9 is a novel and specific marker for the prospective isolation of MSC from human term PL. 相似文献
8.
Source of cell injected is a critical factors for short and long engraftment in xeno-transplantation
G. Noia M. S. Ligato E. Cesari D. Visconti G. Fortunato M. Tintoni I. Mappa C. Greco M. E. Caristo‡ G. Bonanno§ M. Corallo§ L. Minafra§ A. Perillo M. Terzano† S. Rutella§ G. Leone§ G. Scambia¶ M. Michejda S. Mancuso 《Cell proliferation》2008,41(S1):41-50
Abstract. This study aims to investigate engraftment of human cord blood and foetal bone marrow stem cells after in utero transplantation via the intracoelomic route in the sheep. Here, we performed transplantation in 14 single and 1 twin sheep foetuses at 40–47 days of development, using a novel schedule for injection. (i) Single injection of CD34+ human cord blood stem cells via the coelomic route (from 10 to 50 × 104 ) in seven single foetuses. (ii) Single injection of CD34+ foetal bone marrow stem cells via the intracoelomic route with further numbers of cells (20 × 105 and 8 × 105 , respectively) in three single and in one twin foetuses. (iii) Double fractioned injection (20–30 × 106 ) via the coelomic route and 20 × 106 postnatally, intravenously, shortly after birth of CD3-depleted cord blood stem cells in four single foetuses. In the first group, three single foetuses showed human/sheep chimaerism at 1, 8 and 14 months after birth. In the second group, the twin foetuses showed human/sheep chimaerism at 1 month after birth. In the third group, only two out of four single foetuses that underwent transplantation showed chimaerism at 1 month. While foetal bone marrow stem cells showed good short-term engraftment (1 month after birth), cord blood stem cells were able to persist longer in the ovine recipients (at 1, 8 and 14 months after birth). 相似文献
9.
Abstract: Neurotensin and neuromedin N are two structurally related peptides that are synthesized by a common precursor. The purpose of the present work was to characterize neuromedin N receptors in rat and mouse brain and to compare these receptors with those of neurotensin. A radiolabeled analogue of neuromedin N has been prepared by acylation of the N-terminal amino group of the peptide with the 125 I-labeled Bolton-Hunter reagent. This 125 I-labeled derivative of neuromedin N bound to newborn mouse brain homogenate with high affinity (K d = 0.5 n M ). Cross-competition experiments between radiolabeled and unlabeled neurotensin and neuromedin N indicated that each peptide was able to displace completely and specifically the other peptide from its interaction with its receptor. Independently of the radioligand used, the affinity of neurotensin was always better than that of neuromedin N. Quantitative radioautographic studies demonstrated that the ratio of labeling intensities obtained with 125 I-labeled analogues of neurotensin and neuromedin N remained constant in all the brain areas. Our results do not support the existence of a specific neuromedin N receptor in rat and mouse brain and can be explained by the presence of a common receptor for both peptides. 相似文献
10.
Leonardo Ermini Silvia Aldi Floriana Rosati 《Development, growth & differentiation》2008,50(7):577-583
CD52 is a leukocyte differentiation antigen first discovered in humans as expressed on the surface of lymphocytes, monocytes and eosinophils. The human CD52 is found on chromosome 1, and two alleles are both known to be reasonably common. A closely homologous gene has been identified in the cynomologous monkey and related genes have been found in mouse, rat and dog. The role of CD52 in lymphocyte is still unclear but the anti-CD52 antibodies named CAMPATH-1 antibodies are largely used for therapy where depletion of lymphocytes is required. In the past expression of the antigen on progenitors of leukocytes in bone marrow had been excluded, but recent work indicates CD52 is highly expressed on cells with colony-forming and NOD/SCID (non-obese diabetic-severe combined immunodeficiency)-engrafting capacities, both at the mRNA and membrane protein level. We have investigated CD52 expression during development in rat embryos by in situ hybridization. We report here that the antigen is highly expressed in the liver that is the major organ where multipotent hematopietic stem cells differentiate but also in the splancnopleuric mesoderm, at early stages of embryo differentiation, where hematopietic stem cells are suggested to arise. CD52+ cells were found in areas active in vasculogenesis at early embryo stages and in the walls of the vessels in the liver at mid gestation. CD52+ cells were also found to emerge among c-Kit positive cells. 相似文献
11.
Abstract— The distribution of the neuroactive amino acids taurine, GABA, glycine, glutamate and aspartate, together with glutamine, have been studied in the rat retina. Peak levels of taurine were found in photoreceptor cells and of GABA and glycine in a retinal fraction enriched in amacrine cells and, synaptic terminals. In vitro , GABA formation from [3 H]glutamine and [14 C]glucose was also most prominent in this fraction; at 500 μ m [3 H]glutamine was the better precursor.
Observations on metabolism in the photoreceptor cell layer of the tissue suggest an active turnover of glutamate, aspartate and GABA, and show that glutamine may serve as an alternative substrate to glucose here, perhaps via the GABA bypath. 相似文献
Observations on metabolism in the photoreceptor cell layer of the tissue suggest an active turnover of glutamate, aspartate and GABA, and show that glutamine may serve as an alternative substrate to glucose here, perhaps via the GABA bypath. 相似文献
12.
A double isotope DNA labelling method has been used to determine the duration of DNA synthesis (S) in bone marrow lymphoid cells classified by their nuclear diameters in smears. Incorporation of 3 H-thymidine was confined almost entirely to marrow lymphoid cells of 8·0-15·0 μm nuclear diameter (large lymphoid cells). After exposure to 3 H-thymidine in vivo and 14 C-thymidine 40-104 min later in vitro , the proportion of cells labelled with 3 H alone to those labelled with 14 C(±3 H) in radioautographic smears, plotted against time indicated the efflux from S per hour. Collectively, 28·3 ± 1·1% of all large lymphoid cells were in S and the efflux from S was 15·1% per hour. With decreasing cell size (nuclear diameter) the efflux fell progressively from 28·3% per hour (11·0 μm) to 9·2% per hour (8·0-8·9 μm) and the proportion of cells in S declined from 54·9 ± 2·3% to 14·8 ± 1·6%. Influx into S, measured in vitro by reversing the sequence of isotopes, closely resembled the corresponding efflux values in vivo relative to cell size. Most DNA synthesizing marrow large lymphoid cells belonged to a subgroup with deeply basophilic cytoplasm. The results demonstrate that basophilic large lymphoid cells in the marrow are actively proliferating and have a mean S phase duration of 6·6 hr. The largest marrow lymphoid cells (11·0 μm) proliferate most rapidly (S phase, 3·5 hr; maximum cell cycle time, 6·4 hr) while S duration is prolonged progressively to 10·9 hr for the smaller cells (8·0-8·9 μm). 相似文献
13.
Abstract. We have studied carcinoma NT, a transplantable mouse adenocarcinoma of spontaneous origin. Cells labelled with [3 H]thymidine ([3 H]TdR) were restricted to a narrow zone around the periphery of this tumour and were also found in rings up to 50 μ m wide, around isolated blood vessels in the central necrotic area. Labelling with [3 H]deoxyuridine ([3 H]UdR), another DNA synthesis precursor, produced a very different pattern. The labelled zone around the periphery was much wider than with [3 H]TdR, and [3 H]UdR labelled cells were found up to 110 μ m from isolated vessels. [3 H]iododeoxyuridine ([3 H]IUdR) gave the same pattern of labelling as [3 H]UdR. In the heavily labelled zone, within 1 mm of the tumour periphery, the labelling index (LI) was 51% after [3 H]UdR or [3 H]IUdR injection, and only 36% with [3 H]TdR.
The data show that at least half of the DNA-synthesizing cells in this tumour did not incorporate [3 H]TdR. Previous workers reported cell loss factors for carcinoma NT of 60% calculated from [3 H]TdR labelling data and 30% from the rate of loss of [125 I]UdR. The present work suggests that calculations based on [125 I]UdR data are more likely to be accurate for carcinoma NT than those using [3 H]TdR data. 相似文献
The data show that at least half of the DNA-synthesizing cells in this tumour did not incorporate [
14.
O. Vos 《Cell proliferation》1972,5(4):341-350
Kinetics of the multiplication of haemopoietic CFUs was studied in lethally irradiated mice receiving various numbers of syngeneic bone marrow cells. After transplantation of a small number of bone marrow cells, the growth rate of CFU in femoral bone marrow appeared to decrease after about 10 days after transplantation, before the normal level of CFU in the femur was attained. In the spleen it was found that the overshoot which was observed about 10 days after transplantation of a large number of bone marrow cells is smaller or absent when a small number of cells is transplanted. Experiments dealing with transplantation of 50 x 106 bone marrow cells 0, 4 or 10 days after a lethal irradiation indicated that the decline in growth rate of CFUs about 10 days after irradiation could not be attributed to environmental changes in the host.
The results are explained by the hypothesis that a previous excessive proliferation of CFUs diminishes the growth rate thereafter. This hypothesis is supported by experiments in which 50 x 106 bone marrow cells derived from normal mice or from syngeneic chimaeras were transplanted. The slowest growth rate was observed when bone marrow that had been subjected to the most excessive proliferation in the weeks preceding the experiment was transplanted. 相似文献
The results are explained by the hypothesis that a previous excessive proliferation of CFUs diminishes the growth rate thereafter. This hypothesis is supported by experiments in which 50 x 10
15.
Transcription of Repetitive and Unique DNA Nucleotide Sequences in Pigeon Erythroid Cells with Different Degrees of Specialization 总被引:1,自引:0,他引:1
K. G. GASARYAN W. Z. TARANTUL YU. N. BARANOW 《Differentiation; research in biological diversity》1976,6(1-3):41-46
HnRNA fractions with sedimentation coefficients > 45 S isolated from pigeon bone marrow as well as from the immature and mature erythroid cells of periferal blood were hybridised with a large excess of DNA fractionated on the basis of renaturation kinetics. 58–62% of the input RNAs were recovered as RNAase-resistant hybrids. About 1 /3 (20%) of bone marrow > 45 S RNA found in the hybrids was hybridised with the repetitive and about 2 /3 (40%) with the unique DNA sequences. In addition, a considerably smaller portion of > 45 S RNA from the "reticulocytes" (13%) and "eryth-rocytes" (∼ 6%) was hybridised with the repetitive DNA. 相似文献
16.
Abstract. Different sets of cell kinetic data obtained over many years from hairless mouse epidermis have been simulated by a mathematical model including circadian variations. Simulating several independent sets of data with the same mathematical model strengthens the validity of the results obtained. The data simulated in this investigation were all obtained with the experimental system in a state of natural synchrony. The data include cell cycle phase distributions measured by DNA flow cytometry of isolated epidermal basal cells, fractions of tritiated thymidine ([3 H]TdR) labelled cells within the cell cycle phases measured by cell sorting at intervals after [3 H]TdR pulse labelling, bivariate bromodeoxyuridine (BrdUrd)/DNA data from epidermal basal cells isolated at intervals after pulse labelling with BrdUrd, mitotic rate and per cent labelled mitosis (PLM) data from histologic sections. The following main new findings were made from the simulations: the second PLM peak observed at about 35 h after pulse labelling is hardly influenced by circadian variations; the peak is mainly determined by persisting synchrony of a rapidly cycling population with a G1 -duration (TG1 ) of 20 h to 30 h; and there is a highly significant population of slowly cycling G1 -cells (G1σ ). However, no significant circadian variations were found in the number of these cells. 相似文献
17.
Ruth A. Lanius ‡Christopher A. Shaw †Ravenska Wagey † Charles Krieger 《Journal of neurochemistry》1994,63(1):155-160
Abstract: We have characterized a high-affinity [35 S]-glutathione ([35 S]GSH) binding site in mouse and human spinal cord. [35 S]GSH binding sites in mouse and human spinal cord were observed largely within the gray matter in both the dorsal and ventral horns of spinal cord at cervical, thoracic, and lumbosacral segments. High-affinity [35 S]GSH binding was saturable, showing a B max of 72 fmol/mg of protein and a K D of 3.0 n M for mouse spinal cord and a B max of 52 fmol/mg of protein and a K D of 1.6 n M for human spinal cord. [35 S]GSH binding was displaceable by GSH, l -cysteine, and S -hexyl-GSH, but not by glutamate, glycine, or NMDA. These [35 S]GSH binding sites exhibited kinetic and saturation characteristics similar to GSH binding sites in rat brain astrocytes. To determine whether [35 S]GSH binding sites could be regulated by protein kinase C, we exposed human spinal cord sections to phorbol 12,13-diacetate for 1 h before ligand binding. Phorbol ester treatment increased [35 S]GSH binding by ∼60%, an effect that could be blocked by exposure of spinal cord sections to 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine, a general protein kinase inhibitor. [35 S]GSH binding sites in the spinal cord of both species exhibited many of the characteristics of a receptor including saturable binding, high affinity, ligand specificity, and modulation by kinase activity. These data suggest that GSH is a neurotransmitter in the CNS. 相似文献
18.
Abstract. Adult murine bone marrow cells, cultured under conditions for long-term haemopoietic marrow cultures, produce bone matrix proteins and mineralized tissue in vitro , but only after the adherent stromal cells were loaded on a 3-dimensional collagen sponge. Provided more than 8 × 106 cells are loaded, mineralization as measured by 85 Sr uptake from the culture medium, occurred in this 3-dimensional configuration (3-D) within 6 days. In contrast if undisrupted marrow fragments (containing more than 107 cells) are placed directly on a collagen sponge, then it requires more than 10 days before significant mineralization can similarly be detected. The 2-dimensional (2-D) long-term marrow culture system allows prior expansion of the stromal cells and some differentiation in an osteogenic direction within the adherent stromal layer. This is suggested by the presence of type I collagen and alkaline phos-phatase positive cells. However, synthesis of osteonectin and a bone specific protein, osteocalcin, as well as calcification are only observed in 3-D cultures. Electron microscopy demonstrated hydroxyapatite mineral on collagen fibres, osteoblast-like cells, fibroblasts, cells which accumulated lipids, and macrophages which were retained on the collagen matrices. Irradiation of confluent long-term bone marrow cultures, prior to their loading on the collagen sponge showed that haemopoietic stem cells are not necessary for the mineralization. 相似文献
19.
Abstract— [125 I]Diiodo α-bungarotoxin ([125 I]2 BuTx) and [3 H]quinuclidinylbenzilate ([3 H]QNB) binding sites were measured in post-nuclear membrane fractions prepared from whole brains or brain regions of several species. Species studied included Drosophila melanogaster (fruit fly), Torpedo californiea (electric ray), Carassius auratus (goldfish), Ram pipiens (grass frog), Kana cutesheiana (bullfrog), Rattus norvegicus (rat, Sprague-Dawley), Mus muscalus (mouse, Swiss random, C58/J, LG/J), Oryctolagus cuniculus (rabbit, New Zealand Whitc), and Bos (cow). Acetyl-CoA: choline O -acetyltransferase (EC 2.3.1.6) levels were also determined in the post nuclear supernatants and correlated with the number of binding sites.
All species and regions except Drosophila had 16–150 fold more [3 H]QNB binding sites than [125 I]2 BuTx binding sites. Brain regions with the highest levels of [125 I]2 BuTx binding were Drosophila heads (300 fmol/mg), goldfish optic tectum (80fmol/mg), and rat and mouse hippocampus (3040 fmol/mg). The highest levels of [3 H]QNB binding were seen in rat and mouse caudate (1.3–1.6 pmol/mg). Lowest levels of [3 H]QNB and [125 I]2 BuTx binding were seen in cerebellum. The utility of [125 I]2 BuTx and [3 H]QNB binding as quantitative measures of nicotinic and muscarinic acetylcholine receptors in CNS is discussed. 相似文献
All species and regions except Drosophila had 16–150 fold more [
20.
Abstract. The present experiments with [14 C]-thymidine (TdR) and [3 H]-bromo-deoxyuridine (BrdU) using mouse jejunal crypt cells show that the upper limit of the tracer dose of TdR is about 0.5 µg g body weight-1 and that of BrdU is about 5·0 µg g body weight-1 . Applying these doses, the proportions of the endogenous DNA synthesis attributed to the exogenous DNA precursor are 2% and 9% respectively. For [3 H]-TdR doses commonly used in cell kinetic studies this proportion is only 0-1-1.0%, a negligible quantity that does not influence the endogenous DNA synthesis. The maximum availability time of tracer doses of TdR as well as BrdU is 40 to 60 min, the majority of the precursors being incorporated after 20 min. The availability time is the same for TdR doses exceeding the tracer dose by a factor of 80, whereas it is prolonged in the case of BrdU doses exceeding the tracer dose by a factor of 50. BrdU is suitable to replace radioactively labelled TdR in short term cell kinetic studies, i.e. determination of the labelling index or of the S phase duration by double labelling. However, more studies are needed to elucidate how far BrdU can replace TdR in long term studies as shown by differences between the fraction of labelled mitoses (FLM) curves of a human renal cell carcinoma measured with BrdU and [3 H]-TdR. 相似文献