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1.
The transmission of the B1 chromosome through females has been analysed in three Moroccan populations (Smir, SO.DE.A. and Mechra) of the grasshopper Eyprepocnemis plorans. We analysed transmission ratio (kB) variation at two levels: intra-individual (to test female age effects) and inter-individual (to test for A chromosome effects). In 81.8% of females, kB did not differ among successive egg-pods, suggesting no effect of female age. The remaining females (18.2%), showed significant differences in kB values among egg-pods, but without clear temporal patterns. In Smir, kB ranged between B elimination (0.244) and B accumulation (0.689) but there was no net accumulation (mean +/- s.e. = 0.463 +/- 0.045). In SO.DE.A., all females analysed transmitted B1 at a Mendelian rate, with a mean kBequal to 0.512 +/- 0.020. In Mechra, kB ranged from 0.341 to 0.972, with mean kB (0.575 +/- 0.029) showing a net B accumulation. All these observations suggest that the B1 chromosome could be at a drive-suppression stage in Smir and Mechra, but that it has already been neutralised in SO.DE.A. 相似文献
2.
B chromosome variation in nine Moroccan populations of the grasshopper Eyprepocnemis plorans was analysed for 3 consecutive years. In addition to B1, which was the predominant B chromosome in all nine populations, we found 15 other B variants, albeit at very low frequency. Eight variants were found in adults caught in the wild, four appeared in adults reared in the laboratory and seven were found in embryo progeny of controlled crosses between a 0B male and a B-carrying female. Some variants were found in more than one kind of material. At least the seven B variants that appeared in embryo progeny of females carrying a different B type arose de novo through mutation of the maternal B chromosome. The mutation rate of B chromosomes was 0.73%, on average, which explains the high variety of morphs and banding patterns found. The most frequent de novo mutations observed in these chromosomes were centromere misdivision with or without chromatid nondisjunction, which generates iso-B-chromosomes or telocentric Bs, respectively, as well as translocations with A and B chromosomes and deletions. But the whole variation observed, including that found in adult individuals, suggests that other mutations such as duplications, inversions and centric fusions do usually affect B chromosomes. Finally, B chromosome mutation rate was remarkably similar in both Moroccan and Spanish populations, which suggests that it might be dependent on B chromosome intrinsic factors. 相似文献
3.
Perfectti F Corral JM Mesa JA Cabrero J Bakkali M López-León MD Camacho JP 《Cytogenetic and genome research》2004,106(2-4):338-343
The persistence of parasitic B chromosomes in natural populations depends on both B ability to drive and host response to counteracting it. In the grasshopper Eyprepocnemis plorans, the B24 chromosome is the most widespread B chromosome variant in the Torrox area (Málaga, Spain). Its evolutionary success, replacing its ancestral neutralized B variant, B2, was based on meiotic drive in females, as we showed in a sample caught in 1992. In females collected six years later, mean B24 transmission ratio (k(B)) was 0.523, implying a very rapid decrease from the 0.696 observed in 1992. This shows that B24 neutralization is running very fast and suggests that it might most likely be based on a single gene of major effect. 相似文献
4.
Dichroplus elongatus, a widespread South American phytophagous grasshopper, exhibits polymorphisms for supernumerary chromosomes and segments (SS) in natural populations in Argentina. In this paper we review the available information on B chromosome polymorphism in D. elongatus related to geographic distribution, patterns of chromosome variation and influence on sperm formation. In D. elongatus the different forms of supernumerary variants are not independent. The proportion of B-carrying individuals (B prevalence) is negatively correlated with SS10 and positively with SS6 frequencies. The analysis of population structure considering the different supernumerary variants would suggest that the patterns of chromosome variation can not be explained only by random factors. Geographic distribution was analyzed scoring the prevalence of B chromosomes in 13 natural populations collected in three different biogeographical provinces from Northwest (Las Yungas province) and East (Espinal and Pampeana provinces) of Argentina. The detected heterogeneity may be explained by significant differentiation between Northwest and East regions and among populations within Las Yungas and Pampeana provinces. Correlation analysis suggested that B chromosome prevalence is associated with maximum temperature and with latitude. Additional information about the nature of the patterns of B chromosome variation was obtained comparing them with those obtained at the mitochondrial DNA level. The hierarchical analysis of molecular differentiation revealed discrepancy with respect to chromosome differentiation and also suggested that the pattern of B chromosomes may not be explained by historical factors. We also discussed the probable influence on fertility of carriers considering the production of abnormal sperm formation (macro and microspermatids) in relation to the number of Bs per follicle. 相似文献
5.
To improve Brassica nigra, the B-genome donor for Brassica juncea through selective introgression of useful variation from A-genome chromosomes, B. nigra-B. rapa chromosome addition stocks were successfully synthesized for the first time. Resynthesized B. juncea was used as B-genome donor species and A-genome addition stocks were developed by hybridizing sesquidiploid plant (ABB) as female and using B. nigra as the male parent. Various cycles of backcrossing and/or selfing were utilized to isolate plants carrying addition of three A-genome chromosomes in the background of B. nigra. These chromosome addition stocks were characterized by chromosome counts, pollen and seed fertility and chromosome specific microsatellite (SSRs) markers. The chromosome number in different backcross/self generations ranged between 2n=26 and 2n=19 with relatively high frequency of univalents (8-10I) at in meiotic configurations observed, suggesting the role of preferential transmission of A-genome chromosomes. SSRs analysis revealed that B. rapa chromosomes 3 and 4 were the first to get eliminated followed by chromosome 10. Remaining chromosomes were maintained till BC(1)F(4). However, second cycle of backcrossing (BC(2)) led to the elimination of chromosome numbers 1 and 2. BC(2)F(2) plants carried the chromosome numbers 6, 7, 8 and 9. Generation BC(3) having plants with 2n=19 carried chromosome numbers 6, 7 and 8. It is possible that chromosomes 6, 7 and 8 had higher transmission frequency and these were better tolerated by the B. nigra genome. 相似文献
6.
Twenty-seven out of 50 progeny analyses performed with specimens of the grasshopper Eyprepocnemis plorans were informative about the transmission of a supernumerary heterochromatic chromosome segment. The simultaneous presence of a B chromosome in some of the parents involved in the crosses permitted us to test the relationship between both types of supernumerary heterochromatin with respect to their transmission. The results demonstrated that the supernumerary segment is partly eliminated through females possessing B chromosomes. The implications of this in relation to the occurrence of the extra segment in natural populations are discussed.by S.A. Gerbi 相似文献
7.
In previous papers we found that the frequency of B chromosomes in native races of maize varies considerably in different populations. Moreover, we found genotypes that control high and low transmission rates (TR) of B chromosomes in the Pisingallo race. In the present work crosses were made to determine whether the genes controlling B-TR are located on the normal chromosome set (As) or on the B chromosomes (Bs). We made female f.0B × male m.2B crosses between and within high (H) and low (L) B-TR groups. The Bs were transmitted on the male side in all cases. The mean B-TR from the progeny of f.0B (H) × m.2B (H) and f.0B (H) × m.2B (L) crosses was significantly higher than that from f.0B (L) × m.2B (L) and f.0B (L) × m.2B (H) crosses. The results show that the B-TR of the crosses corresponds to the H or L B-TR of the 0B female parents irrespective of the Bs of the male parent. This indicates that B-TR is genetically controlled by the 0B female parent and that these genes are located on the A chromosomes. 相似文献
8.
Marcela Rosato Amilcar M. Chiavarino Carlos A. Naranjo María J. Puertas Lidia Poggio 《American journal of botany》1996,83(9):1107-1112
We selected genotypes of high and low B chromosome transmission rate (TR) in a native race of maize (Pisingallo) from northwest Argentina. We made 20 female OB male IB and 20 f.IB m.OB crosses. The former (GOm) showed a large variation of B TR, with a mean of TR ± SE = 0.52 ± 0.06, ranging from 0.17 to 0.98. In the latter (GOf) the mean was TR = 0.47 ± 0.02 ranging from 0.31 to 0.58. Plants showing the highest and the lowest TR were selected to constitute the progenitors of the G1 generations. We made 19 f.0B m.2B crosses, studying 24–30 plants per cross. The TR of the high (H) and low (L) lines in Glm (GlmH and GlmL) significantly differed (TRH = 0.65 ± 0.03, TRL = 0.40 ± 0.01), indicating that the H and L lines are different groups. The large variation in male TR suggests that preferential fertilization of gametes carrying B chromosomes does not always occur. We also selected plants showing high and low TR in the progenies of f.lB m.OB crosses (GOf), and made 24 f.1B m.0B crosses, studying 23–30 plants per cross. The TRs of the H and L lines in G1f (GlfH and GlfL) were significantly different (TRH = 0.48 ± 0.025, TRL = 0.40 ± 0.02). The TRs in G0f and G1fL were significantly different (TR = 0.47 ± 0.02 and 0.40 ± 0.02, respectively), while this was not the case between G0f and G1fH. Our results demonstrate the existence of genotypes controlling B TR in this native population of maize. 相似文献
9.
The common vole Microtus arvalis (the form obscurus) exhibits polymorphism of a pericentric inversion in chromosome pair 5 throughout the species range. In the Urals populations, the frequency of an acrocentric variant of the heteromorphic chromosome is very low (on average 3.2%) and virtually does not change annually. The factors of maintaining stable chromosomal polymorphism in the common vole were studied under conditions of a laboratory colony. Heterozygous and homozygous for the acrocentric chromosome females showed a significant reduction of the reproductive output irrespective of the male karyotype. This effect was manifested mostly in litter size at birth. A number of cytogenetic and exophenotypic characteristics, as well as parent--offspring transmission of this chromosome in crosses of various types, were examined. We have found meiotic drive in favor of the acrocentric, as a result of which the frequency of the acrocentric (without taking into account the postnatal mortality) totaled over all cross variants (0.48) was significantly higher than that expected with random segregation (0.42). It is likely that meiotic drive of the acrocentric largely compensates for the reduced fertility of its carriers, being among the factors of maintaining it in natural populations. 相似文献
10.
Seventeen controlled crosses in which the mitotically unstable B chromosome of Locusta migratoria was carried by one parent only have provided evidence that B chromosomes are significantly eliminated during sexual transmission in males, at a mean rate that almost counteracts the premeiotic accumulation derived from mitotic instability during germ line development. On the other hand, B chromosomes are significantly accumulated in females, presumably by their preferential migration to the secondary oocyte during the first meiotic division. These results substantially change the current knowledge about this B chromosome system, because the main B accumulation occurs in females and not in males, as was hitherto thought. Furthermore, this case shows that the maintenance of a single B system in natural populations may be the result of many different forces and mechanisms acting for and against B chromosomes. 相似文献
11.
The common vole Microtus arvalis (the form obscurus) exhibits polymorphism of a pericentric inversion in chromosome pair 5 throughout the species range. In the Urals populations, the frequency of an acrocentric variant of the heteromorphic chromosome is very low (on average 3.2%) and virtually does not change annually. The factors of maintaining stable chromosomal polymorphism in the common vole were studied under conditions of a laboratory colony. Heterozygous and homozygous for the acrocentric chromosome females showed a significant reduction of the reproductive output irrespective of the male karyotype. This effect was manifested mostly in litter size at birth. A number of cytogenetic and exophenotypic characteristics, as well as parent-offspring transmission of this chromosome in crosses of various types, were examined. We have found meiotic drive in favor of the acrocentric, as a result of which the frequency of the acrocentric (without taking into account the postnatal mortality) totaled over all cross variants (0.48) was significantly higher than that expected with random segregation (0.42). It is likely that meiotic drive of the acrocentric largely compensates for the reduced fertility of its carriers, being among the factors of maintaining it in natural populations. 相似文献
12.
Astyanax scabripinnis, a small neotropical freshwater fish, is a headwater species living in small tributaries of many Brazilian rivers, where they form isolated populations. This species harbors a B chromosome system in several populations. Among the several kinds of Bs reported in this species, the B(M) variant, a large metacentric of a similar size to the largest A chromosome, is the most widespread in natural populations. It probably corresponds to the ancestral B type in this species and a very similar B chromosome is also found in other Astyanax species. Strong evidence suggests that this B is an isochromosome showing structural and functional homology between its two arms, as shown by satellite DNA localization and the formation of a ring B univalent during meiosis. The B(SM) and B(m) variants, a large submetacentric and a small metacentric, respectively, represent rare variants and may be derived from structural rearrangements of the B(M) chromosome. In addition, B microchromosomes (B(micro)) were found in some populations. Frequency analyses in mountain populations have shown that B chromosomes are found in populations located at high altitude, but are absent in populations at low altitude, which is consistent with their parasitic nature, given the ecological peculiarities of both kinds of populations. 相似文献
13.
Simultaneous chromosome polymorphisms for supernumerary elements allow us to analyse the relationships among different forms of heterochromatic variation in nature. We report simultaneous variation patterns for supernumerary segments in chromosomes S10 (SS10), S9 (SS9) and S6 (SS6) and B chromosomes in nine populations of the grasshopper Dichroplus elongatus from two biogeographic provinces from east Argentina. Our results show spatial chromosome differentiation for three out of four supernumerary heterochromatic variants (B chromosomes, SS6 and SS10). The incidence of B chromosomes was negatively correlated with the SS10 frequency. The distribution pattern analysis shows different degree of differentiation among populations for each supernumerary heterochromatic variant suggesting that the detected chromosome variation cannot be explained by interaction between migration and genetic drift. Moreover, the observed population chromosome differentiation was not in agreement with the hierarchical analysis of molecular of heterogeneity at mitochondrial DNA level (mtDNA). The present results point out the importance of the interaction among heterochromatic variants in the chromosome intraspecific variation in east Argentina natural populations of the grasshopper D. elongatus. 相似文献
14.
15.
Muñoz-Pajares AJ Martínez-Rodríguez L Teruel M Cabrero J Camacho JP Perfectti F 《Genetics》2011,187(3):853-863
B chromosomes are dispensable chromosomes found in >2000 eukaryotic species, usually behaving as genomic parasites. Most B chromosomes seem to be made up of the same kind of DNA sequences present in the A chromosomes. This sequence similarity makes it difficult to obtain specific molecular probes that may permit B-presence diagnosis without cytogenetic analysis. We have developed a sequence-characterized amplified region (SCAR) marker for B chromosomes in the grasshopper Eyprepocnemis plorans, which specifically amplifies a 1510-bp DNA fragment exclusively in B-carrying individuals. Fluorescent in situ hybridization and fiber FISH analyses showed that this marker is a tandemly repeated DNA sequence closely intermingled with 45S rDNA. PCR reactions showed the presence of SCAR-like sequences in the A chromosomes, but in two separate fragments, supporting the intraspecific origin of B chromosomes in this species. SCAR marker DNA sequence showed to be identical in B chromosome variants from several localities from Spain and Morocco, and it was very similar to those found in B chromosome variants from Greece and Armenia. This strongly suggests that this sequence was already present in the ancestral B chromosome of this species. In addition, the scarce sequence variation observed among several B variants from very distant populations suggests either a functional constraint or, more likely, a recent and unique origin for B chromosomes in this species. 相似文献
16.
The transmission ratio (ks) for a supernumerary chromosome segment was studied in a total of 54 heterozygous females collected from two Spanish natural populations of the grasshopper Eyprepocnemis plorans. Our analysis clearly demonstrated that ks is negatively dependent on the number of B chromosomes in the female. The possible mechanisms by which B chromosomes may cause undertransmission of the supernumerary segment, and the implications of this phenomenon for the maintenance of this extra chromosome segment, are discussed. 相似文献
17.
Achbani EH Hafidi M Abdellatif B Lamaaraf N Kouta B Nafie N Corbière R Andrivon D 《Communications in agricultural and applied biological sciences》2005,70(3):247-252
Late blight caused by Phytophthora infestans, is the most important disease of potato in Morocco. Use of partially resistant cultivars should be an essential component of a sustainable management strategy of potato late blight, provided the durability of this form of resistance. It is therefore important to determine the nature of P. infestans Moroccan populations. Mating types were determined for 91 strains of P. infestans collected in the northern (Larache-northern plain), north western (Kénitra) and north eastern (Méknès, Middle Atlas) potato cropping areas of Morocco in 1999-2000, 2000-2001 and 2003-2004. They showed a clear regional structure of these populations, with the presence of both mating types (A1 and A2). Of all isolates collected since 1999, A2 mating type constituted 56% (54 isolates), following by A1 mating type (40.7%, 31 isolates) and A1-A2 (self-fertile) mating type (3.30%, 3 isolates). Populations from Méknès and Kénitra consisted mainly of A2 mating type, whereas populations from Larache predominantly included A1 mating type. Physiological race study revealed the presence of 19 races of P. infestans in the first collection of 25 isolates tested between 1999 and 2001. All known virulence genes were detected in western and northern Moroccan isolates, except virulence for resistance genes R2, R5, and R6 which were absent. All isolates were able to overcome two or more R genes except one isolate (5-1) corresponding to race 1. 相似文献
18.
Within the 2.38-Mb Ath1 region of mouse chromosome 1, 42 of 45 genetic crossovers from crosses between C57BL/6J (B6) and either C3H/HeJ (H) or Mus spretus (SPRET) occurred in four zones (A-D); zone A, 100 kb long, contained a cluster of at least four recombination hotspots. F1 sperm assays indicate that within this "torrid zone" the most active hotspot (A3) can initiate recombination on H and SPRET but not B6 chromosomes. The A3 DNA sequence contains a (G/C)TTT repeat, long stretches of A or T, and a cyclic variation in AT content. Recombination was drastically reduced in a cross between B6 and a B6.SPRET Ath1 congenic strain, but was unaffected in a B6 x B6.H Ath1 congenic cross. Similar nonrandom clustering of hotspots has been observed in yeast and the major histocompatibility complexes of human and mouse. To the extent that torrid zones are a general feature of mammalian genomes, they have considerable implications for genetic mapping strategies in both human populations and mouse crosses. 相似文献
19.
Genotypes Suppressing Meiotic Drive of a B Chromosome in the Mealybug, PSEUDOCOCCUS OBSCURUS
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The rate of transmission (k) of a supernumerary B chromosome in male mealybugs is shown to depend strongly on the chromosome set of maternal origin. When both parents came from an isofemale line in which the frequency of the B chromosome increased rapidly and stabilized at a mean of more than 4.0 B chromosomes per individual, k was 0.92 and 0.95 in two series of crosses. However, when the female parent came from one of two isofemale lines in which the frequency of the B chromosome decreased from 2.0 to 0 in a few generations, k ranged from 0.53 to 0.78. The high ks, which represent a strong meiotic drive, are apparently responsible for the observed increase in the frequency of the B chromosome in several lines from a mean of about 0.5 to more than 4.0 in about 20 generations. The rapid loss of the B chromosome in other lines is attributed to genetic factors which caused the reduction in the rate of transmission of the B chromosome. 相似文献
20.
We have investigated the variation of proteins from crude homogenates of mouse kidneys in several strains of Mus musculus by means of two-dimensional electrophoresis. In this study, we have used the strains C57BL/6J, DBA/2J, CD-1, M. m. castaneus, and M. m. molossinus, as well as offspring from crosses among these strains. Out of the 100 loci screened, we have found nine loci showing interstrain differences. We have been able to identify three proteins as Id-1, Car-2, and Sep-1. The remaining variants are probably new loci in the mouse. Most of the variants (seven) can be mapped to a chromosome. We have found also that differences in the protein pattern as seen on two-dimensional gels are small among subspecies of Mus musculus. 相似文献