Orthotrichum dentatum T.Kiebacher & Lüth sp. nov. (Orthotrichaceae)

A new species of Orthotrichum, O. dentatum T.Kiebacher & Lüth, is described from the European Alps. The new species belongs to the subgenus Pulchella (Schimp.) Vitt, section Diaphana Vent. Orthotrichum dentatum is similar to O. schimperi Hammar but differs from the latter in that it features a hairy calyptra, denticulate to dentate leaf apices and strongly keeled leaves. Orthotrichum dentatum grows as an epiphyte on a variety of deciduous trees. So far, the new species has only been found in Italy and Switzerland.     

Tabanidae (Diptera) of Maranh?o state,Brazil. V. Description of Protosilvius gurupi sp. n. (Pangoniinae,Pangoniini) and key to Protosilvius species

Protosilvius gurupi sp. n. (Tabanidae, Pangoniinae) is described and illustrated based on seven female and 53 male specimens collected in the Amazonian region at Reserva Biológica Gurupi, Centro Novo do Maranhão municipality, northwest Maranhão, Brazil. This is the first record of Protosilvius in northern Brazil and in the Amazon Basin. An illustrated key to all Protosilvius species is also presented.     

<Emphasis Type="Italic">In vitro</Emphasis> preservation and micropropagation of <Emphasis Type="Italic">Oreocharis mileense</Emphasis> (W.T. Wang) M. Möller &amp; A. Weber (Gesneriaceae) through shoot organogenesis

Oreocharis mileense (W.T. Wang) M. Möller & A. Weber is endemic to China and was considered to be extinct because it had not been seen in the wild since the first collection in 1906. In 2006, the species was rediscovered in Shilin County, Yunnan Province. Oreocharis mileense was considered critically endangered for its narrow geographic range and extremely small population. An efficient method to preserve plant germplasm by in vitro culturing of O. mileense has not been reported. In this study, an orthogonal array with three factors (6-benzyladenine, BA; α-naphthaleneacetic acid, NAA; and sucrose), at four levels was performed, and shoot induction as well as shoot proliferation were recorded. The results were analyzed to determine the most significant components and the optimum combination for micropropagation of O. mileense. The results showed that: (1) organogenesis was easily induced by different combinations of plant-growth regulators and sucrose; (2) NAA and sucrose had the most significant effect on shoot induction and shoot multiplication, and (3) the optimum induction and proliferation media were 0.5 mg L^?1 BA + 0.2 mg L^?1 NAA?+?30 g L^?1 sucrose and 1 mg L^?1 BA + 0.1 mg L^?1 NAA?+?30 g L^?1 sucrose, respectively.     

Redescriptions of Nereis oligohalina (Rioja, 1946) and N. garwoodi González-Escalante & Salazar-Vallejo, 2003 and description of N. confusa sp. n. (Annelida,Nereididae)

Type material of several polychaete species described by Enrique Rioja from Mexican coasts are lost, and the current status of some species is doubtful. Nereis oligohalina (Rioja, 1946) was described from the Gulf of Mexico, but it has been considered a junior synonym of Nereis occidentalis Hartman, 1945, or regarded as a distinct species with an amphiamerican distribution. On the other hand, Nereis garwoodi González-Escalante & Salazar-Vallejo, 2003, described from Chetumal Bay, Caribbean coasts, could be confused with Nereis oligohalina. In order to clarify these uncertainties, Nereis oligohalina is redescribed based on specimens from the Mexican Gulf of Mexico, including a proposed neotype; further, Nereis garwoodi is redescribed including the selection of lectotype and paralectotypes, and Nereis confusa sp. n. is described with material from the Gulf of California. A key for the identification of similar species and some comments about speciation in nereidid polychaetes are also included.     

The ichnogenous<Emphasis Type="Italic">Palaxius</Emphasis> (crustacean coprolite) and description of<Emphasis Type="Italic">P. hydranensis</Emphasis> n. sp. from the Upper Triassic (Norian part of “Pantokrator” limestone) of Hydra (Greece)

The fossil coprolite ichnogenusPalaxius is known with 22 species from the Permian up to the Tertiary. The diagnostic characteristics and the stratigraphie range of all species is given in this paper. The ichnogenusAgantaxia Kristan-Tollmann is a younger synonym of the ichnogenusPalaxius. The new crustacean coprolitePalaxius hydranensis n. sp. is described from the reef limestones of Norian part of “Pantokrator” limestone of the Island Hydra (Greece). This is the first report of coprolites from Hydra. The organism association of coprolite bearing limestone is mentioned.      

<Emphasis Type="Italic">Sarcocystis menglaensis</Emphasis> n. sp. (Apicomplexa: Sarcocystidae) from Williamson’s mouse deer <Emphasis Type="Italic">Tuagulus williamsoni</Emphasis> (Kloss) (Artiodactyla: Tragulidae)

Williamson’s mouse deer, Tuagulus williamsoni (Kloss), is one of the smallest ungulates among tragulid species found in northern Thailand, and Yunnan Province, China. Here we describe Sarcocystis menglaensis n. sp., infecting two of 14 (14.3%) Williamson’s mouse deer from south-western China. By light microscopy, sarcocysts of S. menglaensis are microscopic, up to 2,170 μm in length, and have a striated sarcocyst wall with 1.5–3.6 μm long palisade-like protrusions. Transmission electron microscopy observations revealed that sarcocyst wall is of “type 10f”, and has numerous villar protrusions folded over the cyst wall. The villar protrusions contained microtubules dispersed throughout the protrusions. Phylogenetic analysis based on 18S rDNA and mitochondrial cox1 gene sequences indicated that S. menglaensis shared a close affinity with species of Sarcocystis Lankester, 1982 from ruminants, which utilise felids as definitive hosts.     

Zur Nomenklatur der Gattungen Porochara Mädler 1955 (syn. Musacchiella Feist & Grambast-Fessard 1984) undFeistiella n. gen. (Charophyta)

The basal plate of the genusPorochara MäDLER 1955 (Porocharaceae, Charophyta) was proved to be divided into two parts by examining its type species. ThusMusacchiella Feist & Grambast-Fessard 1984 is a younger synonym ofPorochara. The genusFeistiella n. gen. is established for those species with undivided basal plates and it is described with the type speciesFeistiella bijuescensis n. sp. Six other species are attributed to this new genus. The relation between a fan shaped internal structure of the spiral cells and a brackish environment is discussed.     

Solving the identity of the large Plagiomnium (Musci) from Madeira (Portugal): P. undulatum var. madeirense T. Kop. & C. Sérgio

A new variety of Plagiomnium undulatum (Hedw.) T. Kop. var. madeirense T. Kop. & C. Sérgio is described from Madeira Island. The plants present an aquatic ecology and are characterised by broadly and longly decurrent leaves and by large size of laminal cells. This taxon is illustrated and the specimens studied are mapped.     

Parastenocaris andreji n. sp. (Crustacea; Copepoda) – the first record of the genus in Slovenia (SE Europe)

Parastenocaris andreji n. sp. was found in ground water in a northwestern part of Slovenia (southern part of Central Europe). Two adult females of the new species were filtered out of 20 000 l of water during examination of an aquifer for water supply. The bore well was 10 m deep. The aquifer is fed by rainwater and probably only occasionally from a nearby alpine river Sava. The new species differs markedly from other known species of the genus by shape and armature of furcal rami, very long and slim maxillae and some characters on endopodites P2–P5. The closest related species are probably Parastenocaris nolliKiefer, 1938 with subspecies P. nolli alpinaKiefer, 1960, P. gertrudae Kiefer, 1968 and P. austriacaKiefer, 1976.     

Metabolomics – an overview. From basic principles to potential biomarkers (part 2)

The metabolome, which represents the complete set of molecules (metabolites) of a biological sample (cell, tissue, organ, organism), is the final downstream product of the metabolic cell process that involves the genome and exogenous sources. The metabolome is characterized by a large number of small molecules with a huge diversity of chemical structures and abundances. Exploring the metabolome requires complementary analytical platforms to reach its extensive coverage. The metabolome is continually evolving, reflecting the continuous flux of metabolic and signaling pathways. Metabolomic research aims to study the biochemical processes by detecting and quantifying metabolites to obtain a metabolic picture able to give a functional readout of the physiological state. Recent advances in mass spectrometry (one of the mostly used technologies for metabolomics studies) have given the opportunity to determine the spatial distribution of metabolites in tissues. In a two-part article, we describe the usual metabolomics technologies, workflows and strategies leading to the implementation of new clinical biomarkers. In this second part, we first develop the steps of a metabolomic analysis from sample collection to biomarker validation. Then with two examples, autism spectrum disorders and Alzheimer's disease, we illustrate the contributions of metabolomics to clinical practice. Finally, we discuss the complementarity of in vivo (positron emission tomography) and in vitro (metabolomics) molecular explorations for biomarker research.