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海边香豌豆胚性愈伤组织的诱导和体细胞胚发生 总被引:2,自引:0,他引:2
将生长14d的海边香豌豆(Lathyrus maritimus(L.)Bigel)无菌苗下胚轴切成0.5cm左右的片段,置于含有1mg/L2,4-D,0.5mg/L BA和0.5%NaCl的MS培养基中,28d后诱导出胚性愈伤组织。将其转入含有适当浓度2,4-D的MS培养基上,又28d后可得到大量球形胚和心形胚以及极少量鱼雷胚和子叶胚。诱导体细胞胚适合的2,4-D浓度为0.5mg/L。较高浓度的2 相似文献
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马尾松成熟合子胚的体细胞胚胎发生和植株再生 总被引:11,自引:0,他引:11
采用马尾松(Pinusm assoniana Lam b.)成熟合子胚为起始外植体,在含2,4-D 10 m g/L,KT和BA 各4 m g/L的DCR培养基上得到胚发生培养物。将白色半透明的愈伤组织(含早期原胚)在含2,4-D1.0 m g/L,KT和BA 各0.4 m g/L的DCR培养基上保持并增殖。在附加9000 m g/L肌醇的DCR高渗培养基上得到粗壮的后期原胚。ABA 和活性炭同时使用能促进子叶胚的形成,最高频率为35.1% 。在无激素培养基上,成熟体细胞胚萌发并进一步形成完整小植株 相似文献
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硬粒小麦单倍体原生质体培养及植株再生 总被引:4,自引:1,他引:3
由硬粒小麦(Triticum durum Desf.)×玉米(Zea maysL.)建立的单倍性胚性愈伤组织,在继代培养4 个月后置于含2.0 m g/L2,4-D、3% 蔗糖、200 m g/L水解酪蛋白、146 m g/L谷氨酰胺和300 m g/L天冬氨酸的MS液体培养基中进行悬浮培养,4 个月后形成了生长迅速、由大小不同(0.5 ~5 m m )的愈伤组织块组成的愈伤组织悬浮系。酶解试验表明,2.0% 纤维素酶RS和0.5% 的离析酶效果最好,而液体悬浮培养物和固体培养的愈伤组织(在酶解时用锋利的解剖刀片切成1 m m 左右的小块)都能释放出大量原生质体,但悬浮培养物释放出的原生质体状态较好,胞质更浓厚,用KM8p 培养基以琼脂糖包埋培养方式培养时分裂频率可达5% 左右。由原生质体再生的小愈伤组织经增殖、筛选后可获得胚性愈伤组织,将其转移至分化培养基Ⅰ(0.2 m g/L 2,4-D、1.0 m g/L BAP、0.1 m g/LNAA、3% 蔗糖、200 m g/L 水解酪蛋白、146 m g/L谷氨酰胺和300 m g/L天冬氨酸的MS固体培养基)和Ⅱ(不含2,4-D,其它成分同Ⅰ)上进行分步分化培养可再生出完整植株,分化频率约为20% 相似文献
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菜心下胚轴原生质体培养和植株再生 总被引:6,自引:0,他引:6
以萌发3—4 天(长约4 cm )的菜心(Brassica campestris var.parachinesis)无菌苗苍白下胚轴为材料,酶解分离原生质体。经纯化的原生质体,在含0.5 m g/LZT、0.5 m g/L2,4-D、1.0 m g/LNAA 和0.4 m ol/L葡萄糖的K8p 培养基中,进行微滴培养。在起始培养14—18小时,原生质体再生新的细胞壁。36 小时再生细胞开始第一次分裂。第三天分裂细胞频率可达35% 。培养第8—9 天,可见含8—16个细胞的小细胞团,植板率为15% —18% 。3 周后将发育成直径为2 m m 的白色小愈伤组织,转到含0.3 m g/L 2,4-D并用gelrite半固化的培养基上,增殖成4—5 m m 直径的愈伤组织。在MS+ 3.2(或1.6) m g/L BA+ 1.6(或0.8) m g/LZT+ 0.01 m g/L NAA+ 0.1 m g/LGA3 和0.2% 蔗糖的分化培养基上,获得芽的分化。切下约2 cm 长的芽苗,转移到含0.2 m g/LIAA 和2% 蔗糖的培养基上,生根形成完整植株 相似文献
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杨树新品种叶肉原生质体培养和植株再生 总被引:4,自引:1,他引:3
从1 个月龄的NL-80106 杨(Populusdeltoides×P. sim onii)无菌苗叶片分离得到大量原生质体,纯化后其原生质体产量为4×107/g fr.w t. 纯化的原生质体在含2,4-D 2 m g/L、NAA 0.5 m g/L和KT 0.5 m g/L的KM8p 和MS培养基中进行高密度液体浅层培养,渗透势为0.40 m ol/L的KM8p 培养基中原生质体分裂频率最高. 培养第5 天观察到第一次细胞分裂,培养10 d 的分裂频率为4.5% ,12 周内可形成大量的细胞团和小愈伤组织. NL-80106杨叶肉原生质体在富含有机氮并以葡萄糖为碳源的培养基中具有较高的分裂频率和植板率.小愈伤组织在gelrite 固化的NLZ1 培养基上增殖生长,3 周后形成4—6 m m 结构紧密的鲜红色愈伤组织,转至NLF分化培养基,分化成苗率为100% . 待芽伸长到3 cm 时,从基部切下转至1/2 MS培养基上诱导生根,形成完整植株 相似文献
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毛花猕猴桃原生质体再生植株 总被引:12,自引:0,他引:12
从毛花猕猴桃(Actinidia eriantha Benth.)试管培养的实生苗新展开叶片分离的原生质体,培养在液体MS(除去NH4NO3)附加2,4-D 1.0 m g/L和葡萄糖0.4 m ol/L的培养基上。培养3周后植板率达到19.4% 。在未添加新鲜培养基的情况下,原生质体再生的细胞可持续分裂,并于3个月时长成2 m m 大小的愈伤组织。将该愈伤组织转移到附加玉米素0.5 m g/L和IAA 0.1 m g/L的固体MS培养基上,分化出苗。试管苗经诱导生根,长成完整小植株 相似文献
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对12个品种的二棱大麦(Hordeumdistichum2n=24)幼穗和6个品种的幼胚在附加500mg/L酪蛋白,150mg/L天门冬素和2mg/L2,4-D的MS培养基(M_(320))中进行了离体培养,并诱导产生出了愈伤组织。把由D_1幼胚所获得的愈伤组织转移到含2,4-D0.5mg/L(M_(305))培养基中后诱导产生出了体细胞胚性愈伤组织,幼苗和根。筛选出了体细胞胚性无性系。在长达二年的继代培养中,该细胞系仍保持旺盛的再生和分化成苗能力。 相似文献
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大叶紫花苜蓿愈伤组织原生质体再生植株 总被引:15,自引:0,他引:15
大叶紫花苜蓿下胚轴诱导的愈伤组织在继代培养基上生长快速,易于分散。继代第12d的愈伤组织原生质体的得率为6.5×107/g鲜重。原生质体培养基为SH基本培养基,含有1.0mg/L2,4-0、0.5mg/LBA、2.0g/LCH、2%蔗糖、6%葡萄糖、5mmol/LMES,培养密度为1.0×105/mL。培养至第12d时的原生质体再生细胞植板率为3.7%。由原生质体形成的小愈伤组织在含2.0mg/L2,4-D的MS固体培养基上大量增殖。增殖的愈伤组织转移至2.0mg/L2-ip+0.1mg/LNAA的B5培养基上,形成体细胞胚并发育成完整植株。 相似文献
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一品红离体培养诱导体细胞胚的研究(简报) 总被引:5,自引:0,他引:5
一品红的嫩茎离体培养在附加不同浓度的2,4-D和6-BA的MS培养基上,2.4D浓度为1.0~2.0 mg· L~(-1)的愈伤组织生长量最大。这些愈伤组织转移到MS+6-BA1.0 mg·L~(-1)+NAA0.1 mg·L~(-1)的分化培养基上后,产生了胚性愈伤组织,观察到大量不同时期体细胞胚。带有体细胞胚的愈伤组织进一步转移到无激素 MS培养基上,便发育成小植株。 相似文献
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Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis
It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis. 相似文献
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RICHARD E. NORRIS 《Botanical journal of the Linnean Society. Linnean Society of London》1991,106(1):1-40
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera. 相似文献
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JOAN VALUÈS MONTSERRAT TORRELL NÚRIA GARCIA JACAS 《Botanical journal of the Linnean Society. Linnean Society of London》2001,137(4):399-407
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted. 相似文献
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肝癌中HBV和HCV基因和抗原的分布及意义 总被引:1,自引:0,他引:1
采用原位分子杂交方法检测HCV
RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV
RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV
RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV
X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV
C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细 相似文献
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H.-R. GREGORIUS 《Biological journal of the Linnean Society. Linnean Society of London》1984,23(2-3):157-165
For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献