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1.
Koroch Adolfina R. Juliani Héctor R. Juliani Héctor R. Trippi Victorio S. 《Plant Cell, Tissue and Organ Culture》1997,48(3):213-217
A method for the micropropagation of Hedeoma multiflorum Benth from shoot tips or nodal segments was developed. Proliferating
microshoot cultures were obtained by placing shoot tips or nodal segments on half-strength Murashige and Skoog (1962) medium
supplemented with 22.2 μM BA or 22.2 μM BA plus 0.05 μM NAA. Individual shoots were excised and transferred into rooting medium
containing auxins (IBA, NAA or IAA). Rooting of shoots was better on half-strength MS medium containing 0.6 μM IAA than on
half-strength MS medium without growth regulators. Rooted plantlets were successfully acclimatized to soil. Preconditioning
at different sucrose concentrations prior to acclimatization had no effect on plant establishment, but influenced plant quality.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
2.
Callus induction and in vitro plantlet regeneration systems for safflower (Carthamus tinctorius L.) cv. Bhima using root,
hypocotyl, cotyledon and leaf explants were optimized by studying the influence on organogenesis of seedling age, media factors,
growth regulators and excision orientation. Supplementation of the medium with an auxin: cytokinin ratio < 1 enhanced the
growth rate of callus cultures; however, for 2,4-D the ratio was > 1.34–11.41 μM concentrations of growth regulators (IAA,
NAA, BA and Kinetin) in the medium were found effective for callus induction and regeneration in all explants. The calli could
be maintained over 32 months. BA (4.43 μM) combined with casein hydrolysate (10 mg l-1) yielded the highest rate of shoot
production on hypocotyl (3–6) and cotyledon (5–7) explants and cotyledonary derived callus (4–8). More shoots were produced
on explants cut from the most basal region of cotyledons from 5 to 7-day-old seedlings than from older seedlings or more distal
cut sites. Apolar placement of explants, inhibited shoot regeneration. The shoot regeneration potential remained upto 7 months
in calli developed on NAA + BA. Of three media tested, MS was superior to SH-M and B5. Rooting of shoots was not efficient;
42% of the shoots were rooted on MS medium containing sucrose (7–8%) + IAA (2.8–5.7 μM). Capitula induction was observed in
both callus mediated shoots on cotyledons and shoots on rooting medium with sucrose, IAA, NAA and IBA. Well developed plantlets
were transferred to the field with a 34% success rate.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
3.
Catapan Elizabete Otuki Michel Fleith Viana Ana Maria 《Plant Cell, Tissue and Organ Culture》2000,62(3):195-202
An efficient micropropagation protocol was developed for the medicinal plant Phyllanthus caroliniensis (Euphorbiaceae) using nodal segments for axillary shoot proliferation. Maximum multiplication (21–23 shoots per explant)
was achieved on MS or AR media supplemented with either 5.0 μM BA, 1.25–5.0 μM kinetin or 2.5–5.0 μM 2iP. Rooting was achieved
with 80–100% of the microshoots on MS medium without growth regulators, although 1.25 μM NAA and 1.25–5.0 μM IAA promoted
significant increases in the number of roots per explant. Regenerated plants were successfully acclimatized and about 88%
of plantlets survived under ex vitro conditions. Flowering was observed on in vitro grown plantlets and after 3–4 weeks of acclimatization. High frequency callus initiation and growth was achieved when nodal
segment explants were inoculated in the vertical position on MS medium supplemented with 5.0 μM 2,4-D. Root cultures were
successfully established on MS medium containing 1.1 μM NAA. The optimized micropropagation, callus and root culture protocols
offer the possibility to use cell/root culture techniques for vegetative propagation and secondary metabolism studies.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
4.
Juliani Héctor R. Koroch Adolfina R. Juliani Héctor R. Trippi Victorio S. 《Plant Cell, Tissue and Organ Culture》1999,59(3):175-179
A method for the micropropagation of Lippia junelliana (Mold.) Tronc. from shoot tips or nodal segments was developed. Proliferating microshoot cultures were obtained by placing
shoot tips or nodal segments on full strength Murashige and Skoog medium (MS) supplemented with 4.4 μM benzyladenine (BA)
or 0.04 μM indolebutyric acid- (IBA) plus 4.4 μM BA. The rooting of shoots was better on full-strength MS medium without growth
regulators. Rooted plantlets were successfully acclimatized to soil. The shoot cultures showed a lower essential oil accumulation
in comparison with parent plants. Essential oil accumulation is closely related with growth and shows a negative correlation
with shoot proliferation.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
5.
Panaia M. Senaratna T. Bunn E. Dixon K.W. Sivasithamparam K. 《Plant Cell, Tissue and Organ Culture》2000,63(1):23-29
A micropropagation protocol was developed for the conservation of the critically endangered Western Australian shrub,Symonanthus bancroftii. It was necessary to screen antioxidant treatments to prevent the occurrence of lethal browning of explants upon excision.
Potassium citrate and citric acid (0.1% w/v in a 4:1 ratio) prevented oxidative browning and was superior to the untreated
control or other antioxidant treatments tested. Half strength Murashige and Skoog (MS) medium containing 0.5 μM kinetin and
0.25 μM benzyladenine produced three-fold multiplication compared to 1.75×, 1.5×, 1.8× and 1× multiplication for 2.5 μM kinetin
+ 0.25 μM benzyladenine, 0.5 μM kinetin + 5 μM gibberellic acid, 1 μM kinetin + 3 μM gibberellic acid and half strength MS
with no plant growth regulators, over 4 weeks. Root production was achieved with indole-3-butyric acid (IBA) and α-naphthaleneacetic
acid (NAA) at 0.5/0.5 μM (31% rooting) and 1.0/1.0 μM (36% rooting), after four weeks. Paclobutrazol (PBZ) at 0, 3.4 (1 mg
1−1), 10.2 (3 mg 1−1), or 17 μM (5 mg 1−1) improved tolerance to desiccation after transfer ofin vitro rooted shoots to soil. PBZ at 10.2 μM increased survival to 90% compared to 50% for those plantlets not treated with PBZ.
The acclimatisation period from the glasshouse to the shadehouse was 1 week for plantlets treated with PBZ compared to 4 weeks
for plantlets without any PBZ. PBZ at 3.4 μM increased the number of roots per shoot compared to untreated controls.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
6.
Camelina sativa was successfully established in vitro and systems for the regeneration of shoots from leaf explants developed.
Methods for the surface-sterilisation of seeds were used which gave 95% germination, though the in vitro grown seedlings failed
to develop beyond 28 days culture. In a micropropagation system, the rooting response of nodal explants was increased from
a control level of 26.4% to 46.7% by the addition of 5.4 μM NAA. Leaf explants were more efficient for the regeneration of
root and shoots than hypocotyls. For regeneration from leaf tissue the use of auxin (NAA) alone in the medium above a level
of 0.54 μM resulted in root or callus growth. Cytokinin, in the form of BA alone failed to induce regeneration, but a combination
of 4.44 μM BA and 0.54 μM NAA induced shoot regeneration at rates over 10.0 shoots per explant. Regenerated shoots were successfully
transplanted to soil and flowered and set seed normally.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
7.
Somatic embryogenesis and plant regeneration from fragments of immature inflorescences and coleoptiles of durum wheat 总被引:4,自引:0,他引:4
Benkirane Hasnae Sabounji Karima Chlyah Averil Chlyah Hassan 《Plant Cell, Tissue and Organ Culture》2000,61(2):107-113
Use of Hypericum perforatum L. has increased in the past few years due to the antidepressant and antiviral activities found in extracts of this plant.
As a result of its potential as a pharmaceutical, a new system was developed for in vitro culture of this species. Leaf explants were inoculated onto MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D,
0.45 or 4.5 μM) and 6-benzyladenine (BA, 0.44 or 4.4 μM) or kinetin (0.46 or 4.6 μM). Explants were cultivated under dark
or light conditions to induce callus formation. Callus initiation was observed in all media evaluated and the highest cell
proliferation was obtained from explants cultivated in the presence of 4.4 μM BA and 4.5 μM 2,4-D in the dark. Shoot induction
was obtained from callus induced on 4.6 μM kinetin and 0.45 μM 2,4-D 6 weeks after transferring the callus to a MS medium
supplemented with 4.4 μM BA. Roots were induced from shoots on full and half-strength MS media with or without indolebutyric
acid (IBA, 4.9 μM) and the highest rooting frequencies were obtained on half-strength MS medium, regardless of the presence
of IBA. Regenerated plants were easily acclimated in greenhouse conditions. The procedure reported here allows the micropropagation
of H. perforatum in five months of culture and the proliferation of cell masses which could be used for studies on organic compounds of pharmaceutical
interest.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
8.
Micropropagation of juvenile sycamore maple via adventitious shoot formation by use of thidiazuron 总被引:4,自引:0,他引:4
Eva Wilhelm 《Plant Cell, Tissue and Organ Culture》1999,57(1):57-60
Zygotic embryos of sycamore maple (Acer pseudoplatanus) were dissected into plumule, hypocotyl and radicle sections. The segments
were placed on MS medium containing 1 μM 6-benzyladenine (BA) and/or 0.02 μM to 0.1 μM thidiazuron (TDZ). Hypocotyl and plumule
explants produced callus, adventitious buds and shoots with increasing plant growth regulator concentrations. Hypocotyls produced
more, but smaller shoots compared to plumule segments. Subculturing excised shoots and calluses on Murashige and Skoog (MS)
media with 1 μM BA and/or 0.04 μM TDZ led to continuous production of shoots. The best proliferation capacity occurred with
0.04 μM TDZ and 1.0 μM BA, both shoots and calluses. This combination showed a stimulatory effect also on length of newly
formed shoots. Calluses performed generally better compared to shoot explants independent of growth regulator treatment. Excised
shoots 2 to 3-cm-long were successfully rooted on MS media either with or without growth regulators (123 μM IBA pulse) followed
by transfer to the greenhouse.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
9.
TDZ-specific plant regeneration in salad burnet 总被引:5,自引:0,他引:5
Various explants of salad burnet (Poterium sanguisorba L.=Sanguisorba minor Scop.) were cultured on semi-solid MS or B5 media containing factorial combinations of various plant growth regulators. Hypocotyl
and petiole explants, after initial callus stage, regenerated prolific adventitious shoots via organogenesis, when placed
on Murashige and Skoog basal medium containing 1–2 μM α-naphthaleneacetic acid and 4-20 μM thidiazuron. Any other growth regulator
combination tested failed to respond. The addition of the biocide, Plant Preservative Mixture, was effective in controlling
contamination and did not impair regeneration. Elongated shoots at 2–4 cm were transferred to rooting medium containing semi-solid
Murashige and Skoog plus 1 μM α-naphthaleneacetic and rooted plants were transferred to the glasshouse. This is the first
report on in vitro plant regeneration within the genusPoterium.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
10.
Efficient in vitro regeneration systems for Vaccinium species 总被引:1,自引:0,他引:1
Julia Meiners Melanie Schwab Iris Szankowski 《Plant Cell, Tissue and Organ Culture》2007,89(2-3):169-176
Efficient protocols for shoot regeneration from leaf explants suitable for micropropagation as well as for the development
of transgenic plants were developed for blueberry (Vaccinium corymbosum) and lingonberry (Vaccinium vitis-idaea) cultivars. Nodal segments were used to initiate in vitro shoot cultures of lingonberry cultivar ‘Red Pearl’ and southern
highbush blueberry cultivar ‘Ozarkblue’. In order to develop an optimized regeneration procedure, different types and concentrations
of plant growth regulators were tested to induce adventitious shoot regeneration on excised leaves from micropropagated shoots
of both cultivars. The effect on percentage regeneration and number of shoots per explant was investigated. Results indicated
that zeatin was superior to TDZ and meta-topolin in promoting adventitious shoot formation. A concentration of 20 μM zeatin
was most effective in promoting shoot regeneration in both cultivars, in case of ‘Red Pearl’ along with 1 μM NAA. Shoots were
either allowed to root in vitro on medium containing IBA or NAA or ex vitro in a fog tunnel. IBA was superior to NAA for induction
of root development in vitro in both Vaccinium cultivars. Ex vitro rooting under high humidity was tested with cuttings from mature field-grown plants, from acclimatized
tissue culture derived plants and with unrooted in vitro proliferated shoots planted directly. It was found that in vitro
shoots rooted better under fog than cuttings from the other plant sources and rooting was equivalent to that achieved in vitro. 相似文献
11.
Pruski Kris W. Lewis Tina Astatkie Tess Nowak Jerzy 《Plant Cell, Tissue and Organ Culture》2000,63(2):93-100
In vitro culture establishment, shoot proliferation and ex vitro rooting responses of chokecherry (Prunus virginiana L.), `Garrington', and pincherry (P. pensylvanica L.f), `Mary Liss' and `Jumping Pound', were examined using various combinations of growth regulators. Dormant winter buds
were used as explants. MSMO medium supplemented with 0.49 μM IBA and either 4.44 or 8.87 μM BA was found to be optimal for
culture initiation of both species and cultivars. GA3 (28.89 μM) significantly reduced (p=0.0001) the number of successfully established cultures. BA concentrations 8.87–12.82 μM gave optimal shoot proliferation
in chokecherry and 4.44 μM BA in both cultivars of pincherry. Auxin treatments were required for ex vitro rooting of approximately 10 mm long shoots in peat/perlite (1:1 v/v) mixture, at 25 °C, under mist. The best rooting (84%)
was obtained with IBA/NAA (9.80/2.69 μM). A commercial rooting powder, Rootone F, containing IBA/NAA (0.057/0.067%) mixture,
was also effective (75%). The ex vitro rooted plantlets did not require any additional acclimatization prior to transplanting to the regular greenhouse conditions.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
12.
Guohua Ma Jaime A. Teixeira da Silva Jinfeng Lü Xinhua Zhang Jietang Zhao 《Plant Cell, Tissue and Organ Culture》2011,105(3):355-361
An efficient propagation and regeneration system via direct shoot organogenesis for an endangered species, Metabriggsia ovalifolia, was established. High activity cytokinins [6-benzyladeneine (BA) and thidiazuron (TDZ)] and low activity auxins [α-naphthaleneacetic
acid (NAA), indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA)] could directly induce adventitious shoots from leaf
or petiole explants within 5 weeks. Cytokinins (TDZ or BA) combined with auxin (NAA) in the induction media induced more adventitious
shoots than when auxins or cytokinins were used alone. Adventitious shoots could be induced and also mass-propagated on media
containing 2.5–5.0 μM TDZ (or BA) and 0.25–0.5 μM NAA. Adventitious roots differentiated at the proximal end of shoots on
rooting media containing half-strength MS salts and 0.5 μM IBA, 0.5 μM NAA, 0.1% activated charcoal or no plant growth regulators.
Over 90% of plantlets survived following acclimatization and transfer to a potting mixture (1:1, sand:vermiculite) in basins. 相似文献
13.
Murashige and Skoog’s (MS) basal medium with benzylaminopurine (BA), kinetin (KN), zeatin (Z), and thidiazuron (TDZ) were
tested for induction of multiple shoots from mature-tree-derived axillary meristems of Pongamia pinnata. Sprouting of buds was 64% on medium devoid of plant growth regulators (PGR). Incorporation of BA, KN, or Z was ineffective
in enhancing sprouting frequency or induction of multiple shoots. Sprouting was completely suppressed in the presence of TDZ.
Caulogenic buds appeared in nodal meristems of these explants after withdrawal of TDZ. The number of shoot buds was more on
explants precultured in higher concentrations. At higher concentrations of this PGR, a swelling developed at the axil. Multiple
shoot primordia appeared and differentiated from this swelling after culturing these explants on MS medium for six passages
of 2 wk each. Shoots were harvested and cultured on 0.45 μM TDZ for further proliferation. Primary explants after harvesting
of shoots were identified as ‘stump’. Reculturing of stumps on 0.45 μM TDZ produced more shoots. This step was followed for
six cycles to obtain additional shoots in each cycle. Shoots maintained on 0.45 μM TDZ elongated and rooted (70%) on growth
regulator-free medium. Rooted shoots (65%) survived transfer to a sand/soil mixture. This report describes the protocol for
micropropagation of P. pinnata using mature-tree-derived nodal meristems. Recycling of mature stock to produce a stream of useable shoots for subculturing
and eventual stabilization is of great value and can possibly be generalized as an isolation protocol especially for woody
species. Repeated proliferation of caulogenic buds from the same origin may also find application in rescue of endangered
germplasm. 相似文献
14.
Janet E. A. Seabrook Bruce G. Cumming 《In vitro cellular & developmental biology. Plant》1977,13(12):831-836
Summary A new, rapid technique for the propagation of amaryllis (Hippeastrum spp. hybrids) by means of tissue culture is reported. Leaf bases, scapes, peduncles, inner bulb scales and ovaries were cultured
successfully in vitro and plantlets were induced readily at various concentrations of growth regulators. Some plantlets also
were produced in the absence of growth regulators. The most productive tissues for propagation were inverted scapes and peduncles,
cultured in a modified Murashige and Skoog salt solution with added organic constituents and 1 mg per 1 (4.5μM) 2,4-dichlorophenoxyacetic
acid (2,4-D) and 1 mg per 1 (4.4μM) 6-benzylaminopurine (BAP). Plantlets induced axenically also grew roots on the generalized
shoot-inducing medium so that no special rooting medium was required. Although friable callus was obtained from ovary tissue
cultured on a medium containing 2 mg per 1 (11μM) naphthaleneacetic acid and 4 mg per 1 (18μM) BAP, it produced shoots after
8 weeks of further subculture on the same medium. An average of 10 rooted plantlets was obtained from each scape or peduncle
explant on the shoot-propagating medium. Thus, if 45 explants are obtained from each bulb, 450 rudimentary plantlets could
be obtained from each mother bulb in 8 weeks of culture. This is a substantial increase over present propagation methods.
This work was supported by a grant-in-aid of research, to Bruce G. Cumming, from the National Research Council of Canada. 相似文献
15.
This paper describes an efficient in vitro micropropagation of Artemisia vulgaris using shoot tip and nodal explants. Among the various growth regulators tested, MS medium and B5 vitamins supplemented with BA (4.44 μM) and KN (2.32 μM) combination was found to yield a better response than BA (4.44–13.32 μM)
or KN (0.46–13.92 μM) alone in the medium. BA and KN combinations produced a maximum of 23.3 shoots per explant with 99.8%
shooting frequency. Multiple shoots raised were elongated on MS medium containing 0.44 μM BA and 1.44 μM GA3. Rooting was highest (98.2%) on MS medium containing 8.56 μM IAA. Rooted plantlets were successfully transferred to plastic
cups containing autoclaved garden soil, farmyard soil and sand (2:1:1) for hardening. After 65 days, the plantlets were transferred
to Botanical Evaluation Garden and maintained. The survival rate of plantlets varied under acclimatization. Plants looked
healthy with no visually detectable phenotypic variations. This is the first report on plant regeneration via organogenesis
of A. vulgaris. 相似文献
16.
In the micropropagation of woody plant species, adventitious root and shoot formation remain some of the major bottlenecks
due to their recalcitrance to in vitro manipulation. Some plant growth regulators may ameliorate these recalcitrant effects
and improve in vitro caulogenic and rhizogenic processes. Shoot induction on shoot meristems, hypocotyls and epicotyls was
evaluated using equimolar concentrations of benzyladenine (BA), meta-topolin (mT), meta-topolin riboside (mTR), and meta-methoxytopolin riboside (MemTR). Three auxins, indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and α-naphthalene acetic acid (NAA) were used in
the induction of adventitious roots. Moderately high shoot formation (62.7%) was achieved at a concentration of 8.0 μM mT after 8 weeks in culture. The highest number of adventitious shoots per explant (2.4 ± 0.3) and the longest shoots (23.5 ± 3.16 mm)
were recorded on 8.0 μM mT, though not significantly different from BA treatments. Most shoots progressively produced brown basal callus, which is
a potential sink for cytokinin conjugates that are inhibitory to further proliferation of adventitious shoots. Good adventitious
shoot formation occurred in 55% of hypocotyl explants on 8.0 μM mT. The highest rooting (91.6%) was achieved with IBA-treated shoots at a concentration of 4.0 μM. The use of mT and IBA provide an efficient micropropagation method for S. birrea, though further research is required especially in overcoming ex vitro plantlet survival challenges. 相似文献
17.
Summary
Salix tarraconensis Pau ex Font Quer, an endemic willow species from northeast Spain, was micropropagated with nodal segments. Shoot multiplication
was obtained with different cytokinins, either on Murashige and Skoog medium or woody plant medium. Best results for shoot
formation were obtained on Murashige and Skoog medium containing 4.9 μM of 6-γ-γ-dimethylallylaminopurine. Shoots showed strong apical dominance, and some cultures displayed apical necrosis. Benzyladenine
gave the worst results; shoots displayed very slow growth, deformed leaves, and hyperhydrity. Good rooting of shoots was obtained
with different auxins or without plant growth regulators on woody plant medium. The best results (90-100%) were obtained within
20 d. On rooting media with indole-3-butyric acid or indoleacetic acid, shoot elongation was good (35-40 mm length). Apical
necrosis was observed in elongating shoots on rooting medium, but this disturbance favored axillary bud sprouting and formation
of new shoots. Shoot length and quality of roots decreased gradually as the concentration of naphthaleneacetic acid increased.
Plant survival was 90% 4 weeks after removal fromin vitro conditions. 相似文献
18.
Epicotyl, petiole, and cotyledon explants derived from 14-d-old seedlings of Albizia odoratissima were cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of either 6-benzylaminopurine
(BAP) solely or in combination with 0.5 μM naphthalene-3-acetic acid (NAA). The percentage of shoot regeneration and the number
of shoots regenerated varied significantly depending on the type of explants used, the concentration of plant growth regulators,
and the orientation of explants on the culture medium. The best response in terms of the percentage of shoot regeneration
was obtained from epicotyls cultured horizontally on MS medium supplemented with 5 μM BAP, whereas the highest number of shoots
per responding explant was recorded on medium containing 2.5 μM BAP and 0.5 μM NAA. Successful rooting was achieved by placing
the microshoots onto MS medium containing 25 μM indole-3-butyric acid (IBA) for 24 h first, then transferring to the same
medium without IBA. Of the various substrates tested, vermiculite was the best for plant acclimatization, as 75% of the plants
survived and became established. 相似文献
19.
Attempts were made to study the effect of thidiazuron (TDZ) on adventitious shoot induction and plant development in Paulownia tomentosa explants derived from mature trees. Media with different concentrations of TDZ in combination with an auxin were used to
induce adventitious shoot-buds in two explant types: basal leaf halves with the petiole attached (leaf explant) and intact
petioles. Optimal shoot regeneration was obtained in leaf explants cultured on induction medium containing TDZ (22.7 or 27.3 μM)
in combination with 2.9 μM indole-3-acetic acid (IAA) for 2 weeks, and subsequent culture in TDZ-free shoot development medium
including 0.44 μM BA for a further 4-week period. The addition of IAA to the TDZ induction medium enhanced the shoot-forming
capacity of explants. The caulogenic response varied significantly with the position of the explant along the shoot axis.
The highest regeneration potential (85–87%) and shoot number (up to 17.6 shoots/explant) were obtained in leaf explants harvested
from the most apical node exhibiting unfolded leaves (node 1). An analogous trend was also observed in intact petiole explants,
although shoot regeneration ability was considerably lower, with values ranging from 15% for petioles isolated from node 1
to 5% for those of nodes 2 and 3. Shoot formation capacity was influenced by the genotype, with regeneration frequencies ranging
from 50% to 70%. It was possible to root elongated shoots (20 mm) in basal medium without growth regulators; however, rooting
frequency was significantly increased up to 90% by a 7-day treatment with 0.5 μM indole-3-butyric acid, regardless of the
previous culture period in shoot development medium (4 or 8 weeks). Shoot quality of rooted plantlets was improved not only
by IBA treatment but also by using material derived from the 4-week culture period. Regenerated plantlets were successfully
acclimatized in the greenhouse 8 weeks after transplanting. 相似文献
20.
Phillip A. Wadl Adam J. Dattilo Lisa M. Vito Robert N. Trigiano 《Plant Cell, Tissue and Organ Culture》2011,106(3):513-516
Pityopsis ruthii is an endangered herbaceous perennial species from the United States. In vitro multiplication of this species can be valuable
for germplasm conservation. Flower receptacles of P. ruthii were cultured on Murashige and Skoog medium (MS) supplemented with 11.4 μM indole-3-acetic acid (IAA) in combination with
2.2, 4.4 or 8.8 μM 6-benzyladenine (BA). Shoots were visible within 14–28 days and three plants were successfully rooted on
MS medium supplemented with 5.7 μM IAA. A two tailed t-test for paired-variates revealed that shoot regeneration on MS medium
amended with 11.4 μM IAA and 2.2 μM BA was significantly higher (P < 0.05) than on other treatments. Leaf explants were also cultured on MS not supplemented with growth regulators or supplemented
with 11.4 μM IAA in combination with 0, 2.2, 4.4 or 8.8 μM BA. Shoots were visible within 21–35 days and one plant was successfully
rooted on MS medium supplemented with 5.4 μM NAA. Shoot regeneration on MS medium augmented with 11.4 μM IAA and 2.2 μM BA
was significantly higher (P < 0.05) than the other treatments according to analysis of variance (ANOVA) with a rank transformation. Hyperhydricity and
rooting of shoots was problematic for explants derived from flower receptacles and leaf tissue, but viable plants were regenerated
using both explants sources indicating the potential role for micropropagation in the ex situ conservation of the species. 相似文献