A method for the isolation and determination of ferric ethylenediamine di(o-hydroxyphenylacetic acid) in soil studies

Summary Contraversal results about the behaviour of Fe-EDDHA in soils is thought to be due to the indirect methods used for determining the iron chelate compound in soils. The purpose of this investigation is to develop a method for isolation of Fe-EDDHA from soil extracts inorder to be determined separatly. A 4% solution of tetra-n-heptylammonium iodide in ethyl alcohol was found effective in forming a water-insoluble but n-amyl alcohol-soluble compound of Fe-EDDHA. It was found that the absorption spectra of the iron chelate extracted with n-amyl alcohol has an absorption maximum of 480 mμ corresponding to that of iron chelate of the aqueous solution. The extracted iron chelate adhers to Beer's low in the range of 0 to 120 ppm of Fe-EDDHA. The method of Fe-EDDHA extraction was found to be selective for the isolation of the iron chelate from other soluble compounds. The procedure was also found to be highly efficient in quantitative isolation of Fe-EDDHA, with percent recovery ranging from 97–100. The incubation study of Fe-EDDHA in different soils indicated that the loss of Fe-EDDHA is associated with the organic matter content in the soil. The colorimetric determination of the total metal chelates did not indicate any replacement of other metals for the chelated iron in the soil.     

Spatial heterogeneity of DTPA-extractable zinc in cultivated soils induced by city pollution and land use

The spatial heterogeneity of DTPA-extractable zinc in the cultivated soils of Shenyang suburbs in Liaoning Province of China was investigated, and its map was drawn by the methods of geostatistics combined with geographic information system. The data of soil DTPA-extractable zinc fitted normal distribution after logarithm transformation, and its semivariogram fitted a spherical model. The semivariogram indicated that the spatial dependence of soil DTPA-extractable zinc content was moderate, with the spatial dependence range of 1.69 km and the fractal dimension of 1.96. Stochastic factors contributed to 49.9% of the spatial variability, while structural factors contributed to 50.1% of it. The spatial heterogeneity of soil DTPA-extractable zinc shown by a kriged interpolation map was deeply influenced by stochastic factors such as city pollution, land use pattern and crop distributions. For example, the average content of Zn in vegetable garden soils was 2.5-4 times as much as in their originated soils, an     

CHANGES OF GLYCEROL CONTENT IN DIAPAUSE LARVAEOF THE ORANGE WHEAT BLOSSOM MIDGE, SITODIPLOSIS MOSELLANA （GEHIN） IN VARIOUS SEASONS

The glycerol contents in diapause larvae of the orange wheat blossom midge, Sitodiplosis mosellana (Gehin), collected from various seasons, were measured. The results showed that there was less glycerol content in larvae during living on the wheat head. Content of glycerol began to increase significantly when the larvae left the wheat head and entered the soil. A change trend of upper- lower- upper- lower in larvae glycerol contents during diapause in soil was observed from June to April of next year. More glycerol could be examined in larvae collected in summer and winter than in spring and autumn. There was not more glycerol in cocooned larvae than that in non-cocooned larvae during various seasons from the point of statistics. Comparing the glycerol content of larvae being diapause in the first year with that of larvae in the second year, there was yet no obvious difference when larvae were collected in the same season belonged to different years. Therefore, it is shown that the content of glycerol in larvae of the wheat midge in diapause is affected mainly by the seasons or diapause intensity.     

Characteristics of soil water distribution and evaluation of recharge rate under different grazing history in the Xilin Gol Steppe北大核心CSCD

Aims In the Xilin Gol Steppe, human-induced grassland degradation and land desertification are becoming increasingly severe. Critical evaluation of its impact on soil water and recharge rate is important for sustainable management of soil health and water resources in the region. Methods In order to determine the effect of different grazing history on dynamics of deep soil moisture contents and precipitation infiltration in the Xilin Gol Steppe, three sites with different grazing history (ungrazed since 1979 or UG79, ungrazed since 1999 or UG99, and continuously grazed or CG) were selected with two sampling spots for each site. The precipitation infiltration was estimated using the chloride mass balance method. Important findings The results showed that: 1) Average soil water content of 0–5 m was 7.1%, 6.9%, and 6.3% for UG79, UG99, and CG, respectively, with no significant difference. In the soil layer of 0–2 m, the soil water content of UG79 was 26.6% and 33.7% higher than that of UG99 and CG, respectively. The soil water content of UG79 was significantly higher than that of UG79 and UG99 (p < 0.05) with no significant difference between UG99 and CG. The soil water storage capacity of UG79 was 87.19 mm higher than UG99 and 82.52 mm higher than CG. In the deep layer of 2–5 m, no significant difference in the soil water content and the water storage among different grazing history. 2) The factors influencing soil water differed among different grazing treatments. The soil water content was mainly affected by the vegetation conditions and soil properties for the 0–2 m soil layer, but by the composition of soil particles for the 2–5 m soil layer. The effect of soil organic matter (SOM) content on soil water increased with time without grazing. Soil water content of the entire soil profile of UG79 was significantly correlated with soil texture and SOM content (p < 0.01). Soil water content of 0–2 m was significantly correlated with SOM content (p < 0.01), soil water content of 2–5 m was significantly correlated with the soil texture (p <0.01), but soil moisture content of UG99 and CG had no significant correlation with SOM content. 3) Annual recharge rate was 5.64, 3.54, and 2.45 mm·a –1 for UG79, UG99 and CG, respectively. The recharge rate increased by 44.5% and 130.2% for the site without grazing for 15 and 35 years, respectively. The recharge rate in the study area ranged from 1.95 to 7.61 mm·a –1 , accounting for only 0.55%–2.13% of the precipitation. In summary, ungrazing treatment can increase soil water retention, total water storage capacity, and recharge. © 2018 Editorial Office of Chinese Journal of Plant Ecology. All Rights Reserved.     

Water-holding capacity of ground covers and soils in alpine and sub-alpine shrubs in western Sichuan, China

The water-holding functions of soils and ground covers in terms of moss and litters in the three major shrubs at different altitude gradients were studied using field investigation. The water-holding functions were measured and expressed with Biomass (t/hm²) of moss and litters, along with their maximal water holding capacity (MWHC, t/hm²) and maximal water holding rate (MWHR, %). The physical characteristics of the soils included bulk density, MWHC, capillary water holding capacity (CWHC), and least water holding capacity (LWHC). The result showed that Rhododendron przewalskii shrub exhibited the highest water-holding capacity among the three types. The average MWHC of the moss, litters, and at a depth of 0–40cm in R. przewalskii at different elevation gradients was 46.73,139.98 t/hm², and 2216.92 t/hm², respectively, whereas the average MWHC of the moss, litters, and soils in Quercus aquifolioides was 1.64, 72.08 t/hm² and 2114.88 t/hm², respectively. There was no moss in Quercus cocciferoides, and the average MWHC of litters and soils at a depth of 0–40 cm at different elevation gradients was 84.55 t/hm² and 2062.83 t/hm², respectively. The biomass and MWHC of the moss layer in R. przewalskii shrub significantly decreased with increasing elevation, whereas the reverse occurred in Q. aquifolioides before the maximum was reached at 3400 m, and then the SCM and MWHC decreased. MWHR of the moss layer in R. przewalskii was higher than that in Q. aquifolioides. The biomass and MWHC of the litters in R. przewalskii and Q. aquifolioides decreased with increasing elevation, whereas the reverse occurred in Q. cocciferoide. Regardless of shrub types, soil bulk density increased significantly with increasing soil depth, whereas MWHC decreased significantly with increasing soil depth. Significant decrease in CWHC and LWHC were found only in certain shrub communities. The MWHC with respect to the 0–40cm soil depth significantly decreased with increasing elevation only in R. przewalskii shrub, whereas there was no significant difference in MWHCs among the different elevation gradients for the other two types.     

Effects of mercury on microbial biomass and enzyme activities in soil

Mercury (Hg) is a persistent soil pollutant that affects soil microbial activity. We monitored the changes in soil microbial biomass and activity of enzymes, including alkaline phosphatase, arylsulfatase, fluorescein diacetate (FDA) hydrolytic activity, and o-diphenol oxidase (o-DPO) in three soils contaminated with different concentrations of Hg. Increasing levels of Hg, from 0.5 to 10 μmol/g of dried soil, generally depressed microbial activity; however, the effects of Hg on soil microbial activity depended on soil type and composition, particularly organic matter content. o-DPO was less affected by Hg than the other three enzymes tested. Our results indicate that the analysis of microbial biomass content and soil-enzyme activities may be used to predict the soil quality contaminated with Hg.     

酸雨对缙云山林地黄壤汞溶出的影响

Forest yellow soil and arable yellow soil in Jinyun Mountain were collected to study the effect of simulated acid rain(adjusted to pH 2.0, 3.0, 4.0 and 5.0) on the Hg leaching from soils by the methods of static extraction and dynamic leaching. The results showed that in forest yellow soils, surface accumulation of Hg occurred, and the accumulated Hg was easier to be leached out than that in arable yellow soil by acid rain. The amount of leached Hg was the largest at pH 4.0. To abate the risk of Hg pollution in water bodies by the Hg leaching from this forest soil, the Mountain should be closed, and timber-felling should be forbidden.     

Soil Organic Matter Dynamics Along a Vertical Vegetation Gradient in the Gongga Mountain on the Tibetan Plateau

Our knowledge about soil organic matter (SOM) dynamics is limited although this is an important issue in the study of responses of ecosystems to global climate changes. Twelve sampling plots were set up every 200 m from 1 700 to 3 900 m along the vertical vegetation gradient along the east slope of Gongga Mountain. Samples were taken from all 12 plots for SOM content measurement, although only 5 of the 12 plots were subjected to radiocarbon measurements. A radiocarbon isotope method and a time-dependent model were used to quantify the SOM dynamics and SOM turnover rates along the vertical vegetation gradient. The results showed that the SOM turnover rate decreased and turnover time increased with soil depth for all vegetation types. The litter layer turnover rates presented a clear trend along the gradient. The litter layer turnover rates decreased with an increase in elevation, except that the litter layer turnover rate of mixed forest was higher than that of evergreen forest. Climatic factors, such as temperature and precipitation, were the main factors influencing the surface soil carbon dynamics. The turnover rates of the subsoil (including the A, B, and C horizons in the soil profiles) along the vertical gradient had no clear trends. The SOM of subalpine shrub and meadow turned over more slowly than that of the forest types in almost all soil horizons. The characteristic of short roots distributing in the upper part of the soil profile leads to different SOM dynamics of shrub and meadow compared with the forest types. Coniferous and mixed forests were susceptible to carbon loss from the young carbon pool, but their long and big roots resulted in high △^14C values of the deep soil profiles and increased the input of young carbon to the deep soil. In evergreen forest, the carbon cumulative ability from the B horizon to the C horizon was weak. The different vegetation types, together with their different modes of nutrient and carbon intake, may be the mechanism conditioning the subsoil organic matter dynamics.     

Effect of ducks on CH4 emission from paddy soils and its mechanism research in the rice-duck ecosystem

The effect of ducks on CH₄ emission from paddy soils and its mechanism were probed in order to decide the optimum number of ducks in the rice-duck ecosystem. Methane emission fluxes from paddy soils were measured by the static box technique. The correlations between methane emission and soil physical and chemical characteristics were also analyzed. The results showed that significant differences (p < 0.01) existed in the dissolved oxygen content of water body in the treatment fields, and the more the ducks, the higher the dissolved oxygen content. Secondly, the soil redox matter content and methanogenic bacteria population of the rice-duck ecosystem reduced more sharply than those of the no-duck rice farming, resulting in a lower methane production. Thirdly, the amount of methane emission differed between the treatments—the more the ducks, the less the methane emission. Other related analyses showed that the negative correlation was significant (p < 0.001) between the methane emission flux and dissolved oxygen content of water body. However, CH4 emission flux had significantly positive correlation (p < 0.01) with the soil redox matter content and rice field methanogenic population.     

Effect of ducks on CH4 emission from paddy soils and its mechanism research in the rice-duck ecosystem

The effect of ducks on CH₄ emission from paddy soils and its mechanism were probed in order to decide the optimum number of ducks in the rice-duck ecosystem. Methane emission fluxes from paddy soils were measured by the static box technique. The correlations between methane emission and soil physical and chemical characteristics were also analyzed. The results showed that significant differences (p < 0.01) existed in the dissolved oxygen content of water body in the treatment fields, and the more the ducks, the higher the dissolved oxygen content. Secondly, the soil redox matter content and methanogenic bacteria population of the rice-duck ecosystem reduced more sharply than those of the no-duck rice farming, resulting in a lower methane production. Thirdly, the amount of methane emission differed between the treatments—the more the ducks, the less the methane emission. Other related analyses showed that the negative correlation was significant (p < 0.001) between the methane emission flux and dissolved oxygen content of water body. However, CH4 emission flux had significantly positive correlation (p < 0.01) with the soil redox matter content and rice field methanogenic population.     

西藏土壤中铜含是及分布

1　引　　言目前 ,西藏的生态环境基本保持原生状态 ,是至今地球上受人类活动影响和污染最少的地区之一 .所以 ,西藏是进行生态环境本底调查和表生地球化学研究最为理想的场所 .西藏的土壤中Ｃｕ含量数据 ,不但有助于进一步研究西藏高原表生环境中Ｃｕ的地球化学特征 ,而且还可以为这一地区环境监测与评价等提供基础信息和依据 ,也可以成为全国乃至全世界土壤生态环境背景的永久性参比资料 .2　材料与方法2 1　供试材料样品采自北起唐古拉山 ,南至亚东、樟木、吉隆、普兰 ,东从金沙江 ,西到班公错—除羌唐高原北部以外的西藏广大地区 ,共计…     

铜陵矿区土壤和油菜中铜的分布特征

采集铜陵市几个矿区周围土壤和油菜作物样品,研究了铜在矿区不同土壤及油菜中的分布特征.结果表明:铜陵矿区周边耕作土壤铜含量普遍较高;矿区周围残坡积母质上发育的旱作土壤和下游水稻土的污染较严重,而距矿区较远的潮土污染水平较低;水稻土与矿区周围残坡积母质上发育的旱作土壤全铜含量相近,前者的铜主要来自受污染的灌溉水并有着较高的生物有效性.3种类型土壤的有效铜与全铜含量均显著相关.供试土壤中铜的平均活化率(有效铜占全铜含量的百分比)为15.0%,且与土壤全铜和有机质含量呈正相关,与土壤pH和锰含量呈负相关.油菜籽和油菜秸秆中铜的平均含量分别为4.0和5.8mg.kg-1.当土壤中有效铜含量较低时,油菜中的铜含量随着土壤中有效铜含量增加而上升的趋势十分明显,当土壤中有效铜含量大于30mg.kg-1时,油菜作物吸收铜的能力下降,油菜富集铜的效率逐渐降低.     

Characterizing cultivable soil microbial communities from copper fungicide-amended olive orchard and vineyard soils

Use of copper-based fungicides has led to an increase in the total Cu content in agriculture soils. The focus of this study was to determine fractionation of Cu and to investigate the structure and the diversity of cultivable bacterial communities in two vineyards (one 25 years old and one 2 years old), one olive orchard and two forest soils. All soils developed on an Oligocene sandstone. The concentration of total Cu in the old vineyard (176.6 mg kg⁻¹) and olive orchard (145.5–296.7 mg kg⁻¹) was from 5 to 10 times higher than in forest soils. The major amount of Cu was found bound to the humic substances in cultivated soils, whereas in forest soils Cu was found in the residual mineral fraction. A relationship was found between the number of cultivable Cu-tolerant bacteria and total Cu content in soil. In the cultivated soils, Cu had a toxicological effect on bacterial community, and thereby Cu-levels > to 145 mg kg⁻¹ could be a risk to soil biota. Microbial communities were analysed by community level physiological profiling (CLPP), using the Biolog system, and by the amplified ribosomal DNA restriction analysis (ARDRA) approach. Only when cell suspensions containing 10⁴ colony-forming units (c.f.u.) were inoculated in each well of Biolog EcoPlates it was possible to discriminate microbial communities from different soil samples. As expected, 16S ARDRA showed that cultivated soils had a lower microbial diversity in respect to forest soils.     

北亚热带森林土壤有效微量元素状况研究

研究了北亚热带25a生栓皮栎林,杉木林,火炬松林,毛竹林和5年生2代杉木萌芽林土壤中B,Mo,Cu,Zn,Fe,Mn的有效含量,在土壤剖面中的分布规律,影响其有效性的土壤因子以及不同林分对土壤微量养分含量的影响。结果表明,在该地区的森林土壤中,有效B,Mo,Cu的平均含量均低于各自临界值,有效Zn平均含量接近临界值,Fe,Mn含量丰富;有效微量养分在土壤剖面中的分布园林分和元素种类而异影响微量元素有效性的主要土壤因子为有机质和有效磷含量;不同林分对土壤有效微量元素含量有显著影响,栓皮栎林土壤中有效微量养分含量最高,2代杉木萌芽林最低,其余林分居中且基本接近。     

新疆棉田土壤质量综合评价方法

以新疆主要棉区为研究对象,测定了哈密、博乐、昌吉、奎屯、石河子、阿克苏及喀什棉田土壤耕层的pH、盐分、有机质、全氮、速效磷、速效钾及Cr、Cu、Zn、As、Pb 共计11个指标,综合分析土壤理化性质和重金属含量,采用土壤质量综合指数(SQI)对新疆主要棉区棉田土壤质量进行综合评价.结果表明: 新疆棉区棉田土壤呈碱性,pH均值为7.87,盐分含量均值为3.44 g·kg^-1,为轻度盐化土壤,有机质和全氮含量均偏低,速效磷、速效钾含量较为丰富,与第二次全国土壤普查数据相比,土壤pH、盐分含量、有机质和全氮均呈下降趋势,土壤速效磷明显增长,部分地区土壤速效钾呈现出不同程度的升高趋势;Cr、Cu、Zn、As、Pb 5种重金属含量分别为45.88、40.66、68.30、12.88、16.68 mg·kg^-1,均未超过国家二级标准,但与新疆土壤元素背景值相比,Cu、Zn、As均有累积现象.当重金属内梅罗综合污染指数(PN)小于0.5时,土壤理化性质越好,土壤综合质量越好.土壤有机质、全氮、Cu、Zn和As是影响新疆棉区棉田土壤质量的重要因素.新疆棉区棉田土壤质量总体属于中等水平,昌吉、奎屯质量最高,SQI为0.52,阿克苏质量最低,SQI为0.31,不同棉区土壤质量呈现为:北疆>东疆>南疆.     

Comparison between a chemical and a biological method to determine the N mineralization potential of temperate-humid region soils

The potentially mineralizable organic N of 33 different soils was estimated by a chemical test (hot extraction with 2N KCl) and the values compared with those previously obtained by a biological method (aerobic incubation in the laboratory). On average, the organic N solubilized by the chemical procedure was significantly lower than that mineralized by a two weeks aerobic incubation for all the soils as a whole. The same was true for soils developed over acid rocks and over sediments. However, the values obtained for the soils developed over limestone and basic rocks were similar by both methods. The values obtained by both methods were not significantly correlated neither when considering all soils together nor when considering different groups according to soil management or parent material. Significant correlations between both methods were only found when the soils were separated into two groups according to their organic N content: soils with less than 400 mg N 100 g^–1 soil and soils with more than 400 mg N 100 g^–1 soil. The organic N solubilized by the chemical procedure was significantly correlated with the hexosamine-N content; however, it was not correlated with the factors that control the biological mineralization of the organic N, except with the soluble Al content. Therefore, the chemical extraction did not seem to address the biologically active N pool in a selective way.     

南京城市土壤重金属含量及其影响因素

研究了南京城市土壤重金属含量、来源及其与土壤性质的关系。结果表明,南京城市土壤中,Fe、Ni、Co、V污染不明显,但受到了不同程度的Mn、Cr、Cu、Zn、Pb污染,其中：Pb污染非常严重;重金属在土壤剖面分布没有规律性;Fe、Ni、Co、V元素主要来源于原土壤物质,Cu、Zn、Pb、Cr元素主要来源于人为输入,Mn可能在不同的土壤中来源不同;Fe、Cr、Ni、Co、V元素含量之间均呈极显著正相关,Cu、Zn、Pb、Cr元素含量之间均呈极显著正相关。Fe、Co、V、Ni含量与粘粒含量、CEC呈极显著正相关;Cu、Zn、Pb含量与粘粒含量呈极显著负相关;Cu、Zn、Pb、Cr含量与有机碳呈极显著正相关,Pb含量与pH呈极显著正相关。     

新疆焉耆盆地辣椒地土壤重金属污染及生态风险预警

从新疆加工辣椒主产地(焉耆盆地)采集105个辣椒地典型土壤样品,测定其中As、Cd、Cr、Cu、Mn、Ni、Pb和Zn等8种重金属元素的含量。采用污染负荷指数(Pollution load index,PLI)、潜在生态风险指数(Potential ecological risk index,RI)和生态风险预警指数(Ecological risk warning index,I_(ER))对辣椒地土壤重金属污染及生态风险进行评价。结果表明:(1)焉耆盆地辣椒地土壤Cd、Cr、Ni、Pb和Zn含量的平均值分别超出新疆灌耕土背景值的1.65、1.40、1.32、3.21、6.42倍。辣椒地土壤Pb和Zn呈现重度污染,Cd、Cr和Ni轻度污染,As、Mn和Cu无污染。(2)土壤PLI平均值为1.40,呈现轻度污染。各重金属元素单项生态风险指数从大到小依次为:Cd、Ni、As、Cu、Pb、Cr、Zn。土壤RI平均值为18.40,属于轻微生态风险态势,IER平均值为-4.78,属于无警态势;博湖县辣椒地污染水平、潜在生态风险程度与生态风险预警等级最高,焉耆县污染水平、潜在生态风险程度与生态风险预警等级最低。(3)辣椒地土壤As、Cd、Pb与Zn主要受到人类活动的影响,Cr、Cu、Mn和Ni主要受到土壤地球化学作用的控制。Cd是焉耆盆地辣椒地生态风险等级最高的重金属元素,研究区农业生产过程中要防范Cd的污染风险。     

Clay Mineralogy of Central Victorian (Creswick) Soils: Clay Mineral Contents as a Possible Tool of Environmental Indicator

The clay mineralogy and heavy metal/metalloid (As, Pb and Cu) contents of soils developed on the various rock units in a central highlands environment in Victoria (Creswick, Australia) have been investigated. The clay minerals identified showed an order of abundance as: kaolinite ? illite > smectite > mixed-layer (ML) ≈ vermiculite. The soil clay mineralogy did not change systematically with depth (0～ 10, 10～ 20 and 20～ 30 cm) and showed large variations spatially. The high proportion of kaolinite was probably due to the removal of 2:1 phyllosilicates by the formation of 1:1 kaolinite through weathering, which also reduced the cation exchange capacity (CEC) and electrical conductivity (EC, soil: water ratio of 1:5) of soils by aging. Soils were classified as silty loam to loam with a low clay size (≤ 2μ m) fraction. The soils were acidic to moderately acidic with pH ranging from 4.5 to 7.1, averaging 5.7. Concentrations of As, Pb, and Cu (average values 24.3, 16.7 and 11.0 mg/kg, respectively) did not show an association with the clay mineral contents except vermiculite. The occurrence of smectite and mixed-layer clay contents, although far lower than kaolinite and illite, contributed significantly to CEC of soils. The study area was affected by mining, high natural background As values dominate the area and the role of clay minerals in fixation of metalloid/metals was found to be less significant. Low organic matter content (average ～ 6.5%), low soil surface area (average ～ 1.0 m²/g) and the high proportion of kaolinite mineral content result in a limited ability to fix heavy metals. The role of Fe oxides appeared to be a key influence in the fixation of As and other potentially toxic metals, rather than the clay minerals, and therefore requires further research. This work highlighted the importance of the determination of types and amounts of clay minerals of natural soils in environmental management.     

公路源重金属对青藏高原高寒草甸土壤线虫群落的影响

为了解重金属对青藏高原高寒草甸土壤线虫群落的影响,在邦杰塘草原利用公路源重金属含量差异,采用高通量测序技术,分析重金属对高寒草甸土壤线虫群落多样性及群落组成的影响。结果表明：在重金属含量较低样本中,土壤线虫群落Shannon指数、Ace指数、Chao指数比重金属含量较高样本均要小,且整体上Shannon指数、Ace指数、Chao指数与重金属含量较高样本差异显著（P<0.05）。重金属含量升高改变了高寒草甸土壤线虫纲水平和目水平的群落结构,使近自然状况下的色矛纲（Chromadorea）、小杆目（Rhabdtida）优势土壤线虫群落转变为一类未分类线虫纲（unclassified_p_Nematoda）、一类未分类线虫目（unclassified_p_Nematoda）的优势土壤线虫群落。重金属Cu、Pb、Zn、Cd与线虫门（Nematoda）和刺嘴纲（Enoplea）正相关,但与色矛纲（Chromadorea）负相关,且对土壤线虫Chao指数的变异解释比例依次减小。研究表明土壤重金属含量对青藏高原高寒草甸土壤线虫群落多样性及组成产生很大影响,但仍需对高寒草甸区域进行系统的土壤线虫调查,并丰富土壤线虫生物信息数据库。