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Xiao Wang Haiyun Xie Yufan Ying Danni Chen Jiangfeng Li 《Journal of cellular and molecular medicine》2020,24(18):10302-10310
Epigenetics has long been a hot topic in the field of scientific research. The scope of epigenetics usually includes chromatin remodelling, DNA methylation, histone modifications, non‐coding RNAs and RNA modifications. In recent years, RNA modifications have emerged as important regulators in a variety of physiological processes and in disease progression, especially in human cancers. Among the various RNA modifications, m6A is the most common. The function of m6A modifications is mainly regulated by 3 types of proteins: m6A methyltransferases (writers), m6A demethylases (erasers) and m6A‐binding proteins (readers). In this review, we focus on RNA m6A modification and its relationship with urological cancers, particularly focusing on its roles and potential clinical applications. 相似文献
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Human METTL16 is a N6‐methyladenosine (m6A) methyltransferase that targets pre‐mRNAs and various non‐coding RNAs 下载免费PDF全文
Philipp Hackert Christof Lenz Henning Urlaub Claudia Höbartner Katherine E Sloan Markus T Bohnsack 《EMBO reports》2017,18(11):2004-2014
N6‐methyladenosine (m6A) is a highly dynamic RNA modification that has recently emerged as a key regulator of gene expression. While many m6A modifications are installed by the METTL3–METTL14 complex, others appear to be introduced independently, implying that additional human m6A methyltransferases remain to be identified. Using crosslinking and analysis of cDNA (CRAC), we reveal that the putative human m6A “writer” protein METTL16 binds to the U6 snRNA and other ncRNAs as well as numerous lncRNAs and pre‐mRNAs. We demonstrate that METTL16 is responsible for N6‐methylation of A43 of the U6 snRNA and identify the early U6 biogenesis factors La, LARP7 and the methylphosphate capping enzyme MEPCE as METTL16 interaction partners. Interestingly, A43 lies within an essential ACAGAGA box of U6 that base pairs with 5′ splice sites of pre‐mRNAs during splicing, suggesting that METTL16‐mediated modification of this site plays an important role in splicing regulation. The identification of METTL16 as an active m6A methyltransferase in human cells expands our understanding of the mechanisms by which the m6A landscape is installed on cellular RNAs. 相似文献
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Lina Worpenberg Chiara Paolantoni Sara Longhi Miriam M Mulorz Tina Lence HansHermann Wessels Erik Dassi Giuseppe Aiello F X Reymond Sutandy Marion Scheibe Raghu R Edupuganti Anke Busch Martin M Mckel Michiel Vermeulen Falk Butter Julian Knig Michela Notarangelo Uwe Ohler Christoph Dieterich Alessandro Quattrone Alessia Soldano JeanYves Roignant 《The EMBO journal》2021,40(4)
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RNA酶促共价修饰研究, 尤其是m6A(6-甲基腺嘌呤), 是RNA生物学研究的一个新兴领域。m6A是真核生物mRNA内部序列中最常见的一种转录后修饰形式, 由包含3个独立组分的复合物mRNA: m6A甲基转移酶催化生成。最新研究发现肥胖相关蛋白FTO可以脱掉m6A上的甲基, 表明该甲基化过程是可逆的。抑制或敲除m6A甲基转移酶会引起重要的表型变化, 但是由于过去的检测方法受限, m6A确切的作用机制目前为止还不甚清楚。二代测序技术结合免疫沉淀方法为大规模检测m6A修饰并研究其作用机制提供了可能。文章主要综述了m6A的发现史、生成机制、组织和基因组分布、检测方法、生物学功能等及其最新研究进展, 并通过比较3种IP-seq技术和数据分析的异同及优缺点, 对m6A这种RNA表观修饰研究中尚未解决的问题进行了讨论。 相似文献
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TianLiang Xia Xingyang Li Xueping Wang YunJia Zhu Hua Zhang Weisheng Cheng MeiLing Chen Ying Ye Yan Li Ao Zhang DanLing Dai QianYing Zhu Li Yuan Jian Zheng Huilin Huang SiQi Chen ZhiWen Xiao HongBo Wang Gaurab Roy Qian Zhong Dongxin Lin YiXin Zeng Jinkai Wang Bo Zhao Benjamin E Gewurz Jianjun Chen Zhixiang Zuo MuSheng Zeng 《EMBO reports》2021,22(4)
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Tao Jiang Jiashuang Li Ping Qian Peng Xue Jin Xu Yanrong Chen Juan Zhu Shunming Tang Qiaoling Zhao Heying Qian Xingjia Shen 《Molecular reproduction and development》2019,86(12):1981-1992
N6‐methyladenosine (m6A) plays a key role in regulating gene expression in myriad organisms. Diapause is an important plastic phenotype that allows insects to survive under specific environmental conditions. However, the diapause molecular mechanism remains unknown. In this study, we analyzed the phylogenetics of genes related to the m6A modification complex in the silkworm (Bombyx mori) based on identified sequences from other organisms. We detected the expression of these genes during different developmental phases from four strains with different voltinism. We also determined total m6A content in cells treated with different diapause hormone concentrations or eggs exposed to hydrochloric acid. Our data revealed that m6A‐modification‐related gene expression and m6A content were greater in diapause‐destinated compared to nondiapause‐destined strains. Our findings suggest that m6A modification may provide significant epigenetic regulation of diapause‐related genes in the silkworm. 相似文献
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Chengshun Li Ziping Jiang Jindong Hao Da Liu Haobo Hu Yan Gao Dongxu Wang 《Genetics and molecular biology》2021,44(2)
N6-methyl-adenosine (m6A) methylation is one of the most common and abundant modifications of RNA molecules in eukaryotes. Although various biological roles of m6A methylation have been elucidated, its role in embryonic development is still unclear. In this review, we focused on the function and expression patterns of m6A-related genes in mammalian embryonic development and the role of m6A modification in the embryonic epigenetic reprogramming process. The modification of m6A is regulated by the combined activities of methyltransferases, demethylases, and m6A-binding proteins. m6A-related genes act synergistically to form a dynamic, reversible m6A pattern, which exists in several physiological processes in various stages of embryonic development. The lack of one of these enzymes affects embryonic m6A levels, leading to abnormal embryonic development and even death. Moreover, m6A is a positive regulator of reprogramming to pluripotency and can affect embryo reprogramming by affecting activation of the maternal-to-zygotic transition. In conclusion, m6A is involved in the regulation of gene expression during embryonic development and the metabolic processes of RNA and plays an important role in the epigenetic modification of embryos. 相似文献
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