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Nélida C. Olave Maximiliano H. Grenett Martin Cadeiras Hernan E. Grenett Paul J. Higgins PhD 《Journal of cellular biochemistry》2010,111(3):720-726
The polyphenol quercetin (Quer) represses expression of the cardiovascular disease risk factor plasminogen activator inhibitor‐1 (PAI‐1) in cultured endothelial cells (ECs). Transfection of PAI‐1 promoter‐luciferase reporter deletion constructs identified a 251‐bp fragment (nucleotides ?800 to ?549) responsive to Quer. Two E‐box motifs (CACGTG), at map positions ?691 (E‐box1) and ?575 (E‐box2), are platforms for occupancy by several members of the c‐MYC family of basic helix‐loop‐helix leucine zipper (bHLH‐LZ) proteins. Promoter truncation and electrophoretic mobility shift/supershift analyses identified upstream stimulatory factor (USF)‐1 and USF‐2 as E‐box1/E‐box2 binding factors. ECs co‐transfected with a 251 bp PAI‐1 promoter fragment containing the two E‐box motifs (p251/luc) and a USF‐2 expression vector (pUSF‐2/pcDNA) exhibited reduced luciferase activity versus p251/luc alone. Overexpression of USF‐2 decreased, while transfection of a dominant‐negative USF construct increased, EC growth consistent with the known anti‐proliferative properties of USF proteins. Quer‐induced decreases in PAI‐1 expression and reduced cell proliferation may contribute, at least in part, to the cardioprotective benefit associated with daily intake of polyphenols. J. Cell. Biochem. 111: 720–726, 2010. © 2010 Wiley‐Liss, Inc. 相似文献
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Functional interrelation of MYC2 and HY5 plays an important role in Arabidopsis seedling development
Moumita Chakraborty Sreeramaiah N. Gangappa Jay P. Maurya Vishmita Sethi Archana K. Srivastava Aparna Singh Siddhartha Dutta Madhusmita Ojha Nisha Gupta Mandar Sengupta Hasthi Ram Sudip Chattopadhyay 《The Plant journal : for cell and molecular biology》2019,99(6):1080-1097
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