共查询到20条相似文献,搜索用时 31 毫秒
1.
Inheritance and Gene Mapping of Resistance to Soybean Mosaic Virus Strain SC14 in Soybean 总被引:4,自引:0,他引:4
Hai-Chao Li Hai-Jian Zhi Jun-Yi Gai Dong-Quan Guo Yan-Wei Wang Kai Li Li Bai Hua Yang 《植物学报(英文版)》2006,48(12):1466-1472
Soybean mosaic virus (SMV) is one of the most broadly distributed diseases worldwide. It causes severe yield loss and seed quality deficiency in soybean (Glycine max (L.) Merr.). SMV Strain SC14 isolated from Shanxi Province, China, was a newly identified virulent strain and can infect Kefeng No. 1, a source with wide spectrum resistance. In the present study, soybean accessions, PI96983, Qihuang No. 1 and Qihuang No. 22 were identified to be resistant (R) and Nannong 1138‐2, Pixianchadou susceptible (S) to SC14. Segregation analysis of PI96983 x Nannong 1138‐2 indicated that a single dominant gene (designated as RSC14) controlled the resistance to SC14 at both V2 and R1 developmental stages. The same results were obtained for the crosses of Qihuang No. 1 × Nannong 1138‐2 and Qihuang No. 22 × Nannong 1138‐2 as in PI96983 × Nannong 1138‐2 at V2 stage, but at R1 stage, the F1 performed as necrosis (a susceptible symptom other than mosaic), F2 segregated in a ratio of 1R:2N:1S, and the progenies of necrotic (N) F2 individuals segregated also in R, N and S. It indicated that a single gene (designated as RSC14Q, to be different from that of PI96983) controlled the resistance to SC14, its dominance was the same as in PI96983 × Nannong 1138‐2 (without symptoms) at V2 stage and not the same at R1 stage. The tightly linked co‐dominant simple sequence repeat (SSR) marker Satt334 indicated that all the heterozygous bands were completely corresponding to the necrotic F2 individuals, or all the necrotic F2 individuals were heterozygotes. It was inferred that necrosis might be due to the interaction among SMV strains, resistance genes, genetic background of the resistance genes, and plant development stage. Furthermore, the bulked segregant analysis (BSA) of SSR markers was conducted to map the resistance genes. In F2of PI96983 × Nannong 1138‐2, five SSR markers, Sat_297, Sat_234, Sat_154, Sct_033 and Sat_120, were found closely linked to RSC14, with genetic distances of 14.5 cM, 11.3cM, 4.3cM,3.2cM and 6cM, respectively. In F2 of Qihuang No. 1 × Nannong 1138‐2, three SSR markers, Sat_234, Satt334 and Sct_033, tightly linked to RSC14Q with genetic distances of 7.2 cM, 1.4 cM and 2.8 cM, respectively. Based on the integrated joint map by Cregan et al. (1999), both RScMand RSC14Q were located between Sat_234 and Sct_033 on linkage with group F of soybean, with their distances from Sct_033 at the same side being 3.2 cM and 2.8 cM, respectively. Therefore, RSC14and RSC14Q might be on a same locus. The obtained information provides a basic knowledge for marker‐assisted selection of the resistance gene in soybean breeding programs and fine mapping and map‐based cloning of the resistance gene. (Managing editor: Li‐Hui Zhao) 相似文献
2.
Dagang Wang Ying Ma Yongqing Yang Ning Liu Chunyan Li Yingpei Song Haijian Zhi 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(3):555-565
Soybean mosaic virus (SMV) in soybean [Glycine max (L.) Merr.] is a destructive foliar disease in soybean-producing countries worldwide. In this study, F2, F2:3, and F7:11 recombinant inbred lines populations derived from Kefeng No.1 × Nannong 1138-2 were used to study inheritance and linkage
mapping of the SMV strain SC8 resistance gene in Kefeng No.1. Results indicated that a single dominant gene (designated R
SC8
) controls resistance, which is located on chromosome 2 (MLG D1b). A mixed segregating population was developed by selfing
two heterozygous plants (RHL153-1 and RHL153-2) at four markers adjacent to the locus and used in fine mapping R
SC8
. In addition, two genomic-simple sequence repeats (SSR) markers BARCSOYSSR_02_0610 and BARCSOYSSR_02_0616 were identified
that flank the two sides of R
SC8
. Sequence analysis of the soybean genome indicated that the interval between the two genomic-SSR markers is 200 kb. QRT-PCR
analysis of the candidate genes determined that five genes (Glyma02g13310, 13320, 13400, 13460, and 13470) are likely involved
in soybean SMV resistance. These results will have utility in cloning, transferring, and pyramiding of the R
SC8
through marker-assisted selection in soybean breeding programs. 相似文献
3.
Tongtong Jin Jinlong Yin Tao Wang Song Xue Bowen Li Tingxuan Zong Yunhua Yang Hui Liu Mengzhuo Liu Kai Xu Liqun Wang Guangnan Xing Haijian Zhi Kai Li 《植物学报(英文版)》2023,65(3):838-853
Soybean mosaic virus (SMV) is one of the most devastating viral pathogens of soybean (Glycine max (L.) Merr). In total, 22 Chinese SMV strains (SC1–SC22) have been classified based on the responses of 10 soybean cultivars to these pathogens. However, although several SMV-resistance loci in soybean have been identified, no gene conferring SMV resistance in the resistant soybean cultivar (cv.) Kefeng No.1 has been cloned and verified. Here, using F2-derived F3 (F2:3) and recombinant inbred line (RIL) populations from a cross between Kefeng No.1 and susceptible soybean cv. Nannong 1138-2, we localized the gene in Kefeng No.1 that mediated resistance to SMV-SC3 strain to a 90-kb interval on chromosome 2. To study the functions of candidate genes in this interval, we performed Bean pod mottle virus (BPMV)-induced gene silencing (VIGS). We identified a recombinant gene (which we named RSC3K) harboring an internal deletion of a genomic DNA fragment partially flanking the LOC100526921 and LOC100812666 reference genes as the SMV-SC3 resistance gene. By shuffling genes between infectious SMV DNA clones based on the avirulent isolate SC3 and virulent isolate 1129, we determined that the viral protein P3 is the avirulence determinant mediating SMV-SC3 resistance on Kefeng No.1. P3 interacts with RNase proteins encoded by RSC3K, LOC100526921, and LOC100812666. The recombinant RSC3K conveys much higher anti-SMV activity than LOC100526921 and LOC100812666, although those two genes also encode proteins that inhibit SMV accumulation, as revealed by gene silencing in a susceptible cultivar and by overexpression in Nicotiana benthamiana. These findings demonstrate that RSC3K mediates the resistance of Kefeng No.1 to SMV-SC3 and that SMV resistance of soybean is determined by the antiviral activity of RNase proteins. 相似文献
4.
Pyramiding multiple genes for resistance to soybean mosaic virus in soybean using molecular markers 总被引:1,自引:0,他引:1
Ainong Shi Pengyin Chen Dexiao Li Cuiming Zheng Bo Zhang Anfu Hou 《Molecular breeding : new strategies in plant improvement》2009,23(1):113-124
Seven strains of Soybean mosaic virus (SMV) and three independent resistance loci (Rsv1, Rsv3, and Rsv4) have been identified in soybean. The objective of this research was to pyramid Rsv1, Rsv3, and Rsv4 for SMV resistance using molecular markers. J05 carrying Rsv1 and Rsv3 and V94-5152 carrying Rsv4 were used as the donor parents for gene pyramiding. A series of F2:3, F3:4, and F4:5 lines derived from J05 × V94-5152 were developed for selecting individuals carrying all three genes. Eight PCR-based markers
linked to the three SMV resistance genes were used for marker-assisted selection. Two SSR markers (Sat_154 and Satt510) and
one gene-specific marker (Rsv1-f/r) were used for selecting plants containing Rsv1; Satt560 and Satt063 for Rsv3; and Satt266, AI856415, and AI856415-g for Rsv4. Five F4:5 lines were homozygous for all eight marker alleles and presumably carry all three SMV resistance genes that would potentially
provide multiple and durable resistance to SMV. 相似文献
5.
Yongqing Yang Guijie Zheng Lu Han Wang Dagang Xiaofeng Yang Yuan Yuan Saihua Huang Haijian Zhi 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(7):1783-1791
Soybean mosaic virus (SMV) is one of the most broadly distributed soybean (Glycine max (L.) Merr.) diseases and causes severe yield loss and seed quality deficiency. Multiple studies have proved that a single dominant gene can confer resistance to several SMV strains. Plant introduction (PI) 96983 has been reported to contain SMV resistance genes (e.g., Rsv1 and Rsc14) on chromosome 13. The objective of this study was to delineate the genetics of resistance to SMV in PI 96983 and determine whether one gene can control resistance to more than one Chinese SMV strain. In this study, PI 96983 was identified as resistant and Nannong 1138-2 was identified as susceptible to four SMV strains SC3, SC6, SC7, and SC17. Genetic maps based on 783 F2 individuals from the cross of PI 96983 × Nannong 1138-2 showed that the gene(s) conferring resistance to SC3, SC6, and SC17 were between SSR markers BARCSOYSSR_13_1114 and BARCSOYSSR_13_1136, whereas SC7 was between markers BARCSOYSSR_13_1140 and BARCSOYSSR_13_1185. The physical map based on 58 recombinant lines confirmed these results. The resistance gene for SC7 was positioned between BARCSOYSSR_13_1140 and BARCSOYSSR_13_1155, while the resistance gene(s) for SC3, SC6, and SC17 were between BARCSOYSSR_13_1128 and BARCSOYSSR_13_1136. We concluded that, there were two dominant resistance genes flanking Rsv1 or one of them at the reported genomic location of Rsv1. One of them (designated as “Rsc-pm”) conditions resistance for SC3, SC6, and SC17 and another (designated as “Rsc-ps”) confers resistance for SC7. The two tightly linked genes identified in this study would be helpful to cloning of resistance genes and breeding of multiple resistances soybean cultivars to SMV through marker-assisted selection (MAS). 相似文献
6.
Soybean aphid resistance genes in the soybean cultivars Dowling and Jackson map to linkage group M 总被引:4,自引:0,他引:4
Yan Li Curtis B. Hill Shawn R. Carlson Brian W. Diers Glen L. Hartman 《Molecular breeding : new strategies in plant improvement》2007,19(1):25-34
The soybean aphid [Aphis glycines Matsumura] is an important pest of soybean [Glycine max (L.) Merr.] in North America. Single dominant genes in the cultivars ‘Dowling’ and ‘Jackson’ control resistance to the soybean
aphid. The gene in Dowling was named Rag1, and the genetic relationship between Rag1 and the gene in Jackson is not known. The objectives of this study were to map the locations of Rag1 and the Jackson gene onto the soybean genetic map. Segregation of aphid resistance and simple sequence repeat (SSR) markers
in F
2:3 populations developed from crosses between Dowling and the two susceptible soybean cultivars ‘Loda’ and ‘Williams 82’, and
between Jackson and Loda, were analyzed. Both Rag1 and the Jackson gene segregated 1:2:1 in the F
2:3 populations and mapped to soybean linkage group M between the markers Satt435 and Satt463. Rag1 mapped 4.2 cM from Satt435 and 7.9 cM from Satt463. The Jackson gene mapped 2.1 cM from Satt435 and 8.2 cM from Satt463.
Further tests to determine genetic allelism between Rag1 and the Jackson gene are in progress. The SSR markers flanking these resistance genes are being used in marker-assisted selection
for aphid resistance in soybean breeding programs.
Trade and manufacturers’ names are necessary to report factually on available data; however, the USDA neither guarantees nor
warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion
of others that may also be suitable. 相似文献
7.
SSR mapping and confirmation of the QTL from PI96354 conditioning soybean resistance to southern root-knot nematode 总被引:6,自引:0,他引:6
Z. Li L. Jakkula R. S. Hussey J. P. Tamulonis H. R. Boerma 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(8):1167-1173
Root-knot nematodes (Meloidogyne spp.) can cause severe yield loss of soybean [Glycine max (L.) Merr.] in the southern production region of the USA. Planting root-knot nematode-resistant cultivars is the most effective
method of preventing yield loss. DNA marker-assisted breeding may accelerate the development of root-knot nematode-resistant
cultivars. RFLP markers have previously been used to identify quantitative trait loci (QTLs) conferring resistance to southern
root-knot nematode [Meloidogyne incognita (Kofoid and White) Chitwood] (Mi) in a F2:3 soybean population created by crossing the resistant PI96354 and the susceptible ’Bossier.’ A major QTL on linkage group
(LG) O conditioning 31% of the variation in Mi gall number and a minor QTL on LG-G conditioning 14% of the gall variation
were reported. With the development of SSR markers for soybean improvement, a higher level of mapping resolution and semi-automated
detection has become possible. The objectives of this research were: (1) to increase the marker density in the genomic regions
of the QTLs for Mi resistance on LG-O and LG-G with SSR markers; and (2) to confirm the effect of the QTLs in a second population
and a different genetic background. With SSR markers, the QTL on LG-O was flanked by Satt492 and Satt358, and on LG-G by Satt012
and Satt505. Utilizing SSR markers flanking the two QTLs, marker-assisted selection was performed in a second F2:3 population of PI96354× Bossier. Results confirmed the effectiveness of marker-assisted selection to predict the Mi phenotypes.
By screening the BC2F2 population of Prichard (3)×G93–9009 we confirmed that selection for the minor QTL on LG-G with flanking SSR markers would
enhance the resistance of lines containing the major QTL (which is most-likely Rmi1).
Received: 29 September 2000 / Accepted: 17 April 2001 相似文献
8.
Wang C Ulloa M Roberts PA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(4):770-777
Host-plant resistance is the most economic and effective strategy for root-knot nematode (RKN) Meloidogyne incognita control in cotton (Gossypium hirsutum L.). Molecular markers linked to resistance are important for incorporating resistance genes into elite cultivars. To screen
for microsatellite markers (SSR) closely linked to RKN resistance in G. hirsutum cv. Acala NemX, F1, F2, BC1F1, and F2:7 recombinant inbred lines (RILs) from intraspecific crosses and an F2 from an interspecific cross with G. barbadense cv. Pima S-7 were used. Screening of 284 SSR markers, which cover all the known identified chromosomes and most linkage groups
of cotton, was performed by bulked segregant analysis, revealing informative SSRs. The informative SSRs were then mapped on
the above populations. One co-dominant SSR marker CIR316 was identified tightly linked to a major resistance gene (designated
as rkn1), producing amplified DNA fragments of approximately 221 bp (CIR316a) and 210 bp (CIR316c) in Acala NemX and susceptible
Acala SJ-2, respectively. The linkage between CIR316a marker and resistance gene rkn1 in Acala NemX had an estimated distance of 2.1–3.3 cM depending on the population used. Additional markers, including BNL1231
with loose linkage to rkn1 (map distance 25.1–27.4 cM), BNL1066, and CIR003 allowed the rkn1 gene to be mapped to cotton linkage group A03. This is the first report in cotton with a closely linked major gene locus
determining nematode resistance, and informative SSRs may be used for marker-assisted selection. 相似文献
9.
J05 soybean was previously identified to carry 2 independent genes, Rsv1 and Rsv3, for "soybean mosaic virus" (SMV) resistance by inheritance and allelism studies. The objective of this research was to confirm the 2 genes in J05 using molecular markers so that a marker-assisted selection can be implemented. The segregation of F(2) plants from J05 x Essex exhibited a good fit to a 3:1 ratio when inoculated with SMV G1. Three simple sequence repeat (SSR) markers near Rsv1, Satt114, Satt510, and Sat_154, amplified polymorphic DNA fragments between J05 and Essex and were closely linked to the gene on soybean molecular linkage group (MLG) F, thus verifying the presence of Rsv1 in J05 for resistance to SMV G1. The presence of Rsv3 in J05 was confirmed by 2 closely linked SSR markers on MLG B2, Satt726 and Sat_424, in F(2:3) lines that were derived from the SMV G1-susceptible F(2) plants and segregated in a 1:2:1 ratio for reaction to SMV G7. Two closely linked markers for Rsv4, Satt296 and Satt542, segregated independently of SMV resistance, indicating the absence of Rsv4 in J05. These SSR markers for Rsv1 and Rsv3 can serve as a useful molecular tool for selection and pyramiding of genes in J05 for SMV resistance. 相似文献
10.
Hao Cheng Hua Yang Dan Zhang Junyi Gai Deyue Yu 《Molecular breeding : new strategies in plant improvement》2010,25(1):13-24
Soybean mosaic disease, caused by soybean mosaic virus (SMV), is one of the most devastating diseases that limit soybean production
throughout the world. Soybean isoflavone synthase (IFS) and flavanone 3-hydroxylase (F3H) genes catalyze the production of
isoflavones and flavonoids, the increase of which is correlated with increased disease resistance. We have cloned, sequenced,
and analyzed the IFS1, IFS2 and F3H genomic regions from 33 Chinese soybean accessions including 16 Glycine soja and 17 Glycine max. High nucleotide diversity and low extent of linkage disequilibrium (LD) in these three genes provided sufficient genetic
resolution for association mapping. As a result, a set of single nucleotide polymorphisms (SNPs) with significant (P < 0.05) association to SMV strain SC-3 and SC-7 resistance were discovered in these genes. Among them, the SNP haplotype
‘TCACAACGA-TACA’ in IFS1 gene was found to be extremely significantly (P < 0.01) associated with SMV SC-3 resistance. After 7 days of SC-3 inoculation, the expression level of IFS1 gene in the two SC-3 resistance accessions that have this significant site continued to increased and reach to 30–160 folds
high, while in the SC-3 susceptible accession which does not carry the significant site the expression level decreased to
near zero. These polymorphisms were corresponding to the trait variance and thus can be considered as the candidate sites
for functional molecular markers for future SMV resistance breeding. 相似文献
11.
12.
Yang DE Jin DM Wang B Zhang DS Nguyen HT Zhang CL Chen SJ 《Molecular genetics and genomics : MGG》2005,274(3):229-234
The maize inbred lines 1145 (resistant) and Y331 (susceptible), and the F1, F2 and BC1F1 populations derived from them were inoculated with the pathogen Pythium inflatum Matthews, which causes stalk rot in Zea mays. Field data revealed that the ratio of resistant to susceptible plants was 3:1 in the F2 population, and 1:1 in the BC1F1population, indicating that the resistance to P. inflatum Matthews was controlled by a single dominant gene in the 1145×Y331 cross. The gene that confers resistance to P. inflatum Matthews was designated Rpi1 for resistance to P. inflatum) according to the standard nomenclature for plant disease resistance genes. Fifty SSR markers from 10 chromosomes were first
screened in the F2 population to find markers linked to the Rpi1 gene. The results indicated that umc1702 and mmc0371 were both linked to Rpi1, placing the resistance gene on chromosome 4. RAPD (randomly amplified polymorphic DNA) markers were then tested in the F2population using bulked segregant analysis (BSA). Four RAPD products were found to show linkage to the Rpi1 gene. Then 27 SSR markers and 8 RFLP markers in the region encompassing Rpi1 were used for fine-scale mapping of the resistance gene. Two SSR markers and four RFLP markers were linked to the Rpi1 gene. Finally, the Rpi1 gene was mapped between the SSR markers bnlg1937 and agrr286 on chromosome 4, 1.6 cM away from the former and 4.1 cM distant
from the latter. This is the first time that a dominant gene for resistance to maize stalk rot caused by P. inflatum Matthews has been mapped with molecular marker techniques. 相似文献
13.
Genetics and molecular mapping of genes for high-temperature resistance to stripe rust in wheat cultivar Xiaoyan 54 总被引:1,自引:0,他引:1
Zhou XL Wang WL Wang LL Hou DY Jing JX Wang Y Xu ZQ Yao Q Yin JL Ma DF 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(3):431-438
Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most widespread and destructive wheat diseases worldwide. Growing resistant cultivars is the preferred means
of control of the disease. The winter wheat cultivar Xiaoyan 54 has high-temperature resistance to stripe rust. To identify
genes for stripe rust resistance, Xiaoyan 54 was crossed with Mingxian 169, a winter wheat genotype susceptible to all Chinese
races of the pathogen. Seedlings and adult plants of the parents and F1, F2, F3 and F4 progeny were tested with Chinese race CYR32 under controlled greenhouse conditions and in the field. Xiaoyan 54 has two recessive
resistance genes, designated as Yrxy1 and Yrxy2, conferring high-temperature resistance. Simple sequence repeat (SSR) primers were used to identify molecular markers flanking
Yrxy2 using 181 plants from one segregating F3 line. A total of nine markers, two of which flanked the locus at genetic distances of 4.0 and 6.4 cM on the long arm of chromosome
2A were identified. Resistance gene analog polymorphism (RGAP) and SSR techniques were used to identify molecular markers
linked to Yrxy1. A linkage group of nine RGAP and two SSR markers was constructed for Yrxy1 using 177 plants of another segregating F3 line. Two RGAP markers were closely linked to the locus with genetic distances of 2.3 and 3.5 cM. Amplification of a set
of nulli-tetrasomic Chinese Spring lines with RGAP markers M8 and M9 and the two SSR markers located Yrxy1 on the short arm of chromosome 7A. The SSR markers Xbarc49 and Xwmc422 were 15.8 and 26.1 cM, respectively, from the gene. The closely linked molecular markers should be useful for incorporating
the resistance genes into commercial cultivars and combining them with other genes for stripe rust resistance. 相似文献
14.
Honglang Yan Hui Wang Hao Cheng Zhenbin Hu Shanshan Chu Guozheng Zhang Deyue Yu 《植物学报(英文版)》2015,57(8):722-729
Soybean mosaic virus (SMV) disease is one of the most serious and broadly distributed soybean (Glycine max (L.) Merr.) diseases. Here, we combine the advantages of association and linkage analysis to i... 相似文献
15.
Identification of a new major QTL associated with resistance to soybean cyst nematode (Heterodera glycines) 总被引:4,自引:0,他引:4
I. Schuster R. V. Abdelnoor S. R. R. Marin V. P. Carvalho R. A. S. Kiihl J. F. V. Silva C. S. Sediyama E. G. Barros M. A. Moreira 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(1):91-96
Resistance of soybean [Glycine max (L.) Merr.] to cyst nematode (SCN) (Heterodera glycines Ichinohe), one of the most destructive pathogens affecting soybean, involves a complex genetic system. The identification
of QTLs associated with SCN resistance may contribute to the understanding of such system. The objective of this work was
to identify and map QTLs for resistance to SCN Race 14 with the aid of molecular markers. BC3F2:3 and F2:3 populations, both derived from an original cross between resistant cv. Hartwig and the susceptible line BR-92–31983 were
screened for resistance to SCN Race 14. Four microsatellite (Satt082, Sat_001, Satt574 and Satt301) and four RAPD markers
(OPAA-11795, OPAE-08837, OPR-07548 and OPY-072030) were identified in the BC3F2:3 population using the bulked segregant analysis (BSA) technique. These markers were amplified in 183 F2:3 families and mapped to a locus that accounts for more than 40% of the resistance to SCN Race 14. Selection efficiency based
on these markers was similar to that obtained with the conventional method. In the case of the microsalellite markers, which
identify homozygous resistant genotypes, the efficiency was even higher. This new QTL has been mapped to the soybean linkage
group D2 and, in conjunction with other QTLs already identified for SCN resistance, will certainly contribute to our understanding
of the genetic basis of resistance of this important disease in soybean.
Received: 12 October 1999 / Accepted: 14 April 2000 相似文献
16.
Panthee DR Pantalone VR Sams CE Saxton AM West DR Orf JH Killam AS 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(3):546-553
Soybean [Glycine max (L.) Merr.] is the single largest source of protein in animal feed. However, a major limitation of soy proteins is their
deficiency in sulfur-containing amino acids, methionine (Met) and cysteine (Cys). The objective of this study was to identify
quantitative trait loci (QTL) associated with Met and Cys concentration in soybean seed. To achieve this objective, 101 F6-derived recombinant inbred lines (RIL) from a population developed from a cross of N87-984-16 × TN93-99 were used. Ground
soybean seed samples were analyzed for Met and Cys concentration using a near infrared spectroscopy instrument. Data were
analyzed using SAS software and QTL Cartographer. RIL differed (P<0.01) in Met and Cys concentrations, with a range of 5.1–7.3 (g kg−1 seed dry weight) for Cys and 4.4–8.8 (g kg−1 seed dry weight) for Met. Heritability estimates on an entry mean basis were 0.14 and 0.57 for Cys and Met, respectively.
A total of 94 polymorphic simple sequence repeat molecular genetic markers were screened in the RIL. Single factor ANOVA was
used to identify candidate QTL, which were confirmed by composite interval mapping using QTL Cartographer. Four QTL linked
to molecular markers Satt235, Satt252, Satt427 and Satt436 distributed on three molecular linkage groups (MLG) D1a, F and
G were associated with Cys and three QTL linked to molecular markers Satt252, Satt564 and Satt590 distributed on MLG F, G
and M were associated with Met concentration in soybean seed. QTL associated with Met and Cys in soybean seed will provide
important information to breeders targeting improvements in the nutritional quality of soybean. 相似文献
17.
以大豆组合科丰1号×南农1138-2衍生的重组自交系(RIL)群体为材料构建遗传连锁图谱, 利用软件 Cartographer V.2.5 采用复合区间作图法检测定位大豆抗虫QTL。以斜纹夜蛾幼虫重为抗性指标, 检测到 1 个与抗虫性有关的 QTL, 位于G20-O连锁群上, 其端距离为31.91 cM, 加性效应估计值为0.0408, 对性状变异的解释率为 11.74%; 以蛹重为抗性指标, 检测到 2 个与抗虫性有关的 QTL, 分别位于G8-D1b+W和G17-L连锁群上, 其端距离分别为 14.71 cM和0.01 cM, 加性效应估计值分别为-0.0139和0.0103, 对性状变异的解释率分别为 11.30%和6.36%。 相似文献
18.
Kumari Surbhi Krishna P. Singh Narendra K. Singh T. Aravind 《Journal of Phytopathology》2021,169(1):37-44
Forty‐seven genotypes and one wild relative of soybean, Glycine soja, were screened for resistance against aerial blight under epiphytotic conditions in the field during the Kharif season of two consecutive years viz., 2016 and 2017. Out of the 48 genotypes screened, only 18 genotypes exhibited a moderately resistant response to aerial blight during both the years of study. In order to perform molecular screening of the genotypes for aerial blight resistance, the genomic DNA obtained from the seedlings of the forty‐eight soybean genotypes was subjected to PCR amplification with 12 SSR markers. The SSR markers Satt 119, Sat_076, Satt 433, Satt 281, Satt 277, Satt 245 and Satt 520 were able to clearly amplify different banding pattern for resistant and susceptible genotypes, out of which Satt 433 and Satt 520 were found to exhibit a pattern, highly similar to the results of field screening of the genotypes with respect to resistant and susceptible reaction to the disease. The eighteen soybean genotypes that exhibited moderately resistant reaction to RAB under field conditions during both the years showed a banding pattern similar to resistant check PS‐1583 in the amplification profile produced by the SSR markers. The polymorphism information content (PIC) from the analysis of amplification profile of the SSR markers used in the study, ranged from 0.58 to 0.95. The dendrogram constructed using UPGMA cluster analysis clearly differentiated the resistant and susceptible genotypes of soybean into two separate groups. 相似文献
19.
大豆重要农艺性状的QTL分析 总被引:55,自引:0,他引:55
应用栽培大豆科丰1号(♀)和南农1138-2(♂)杂交得到的F9代重组自交系(RILs)群体(201个家系),构建了含302遗传标记、覆盖2363.8cM、由22个连锁群组成的遗传连锁图谱。采用区间作图法,对该群体的主要农艺性状的调查数据进行QTL分析,表明与开花期、成熟期、株高、主茎节数、每节荚数、倒状性、种子重、产量、蛋白质和含油量等10个重要农艺性状连锁的QTL位点34个,每个数量性状的遗传变异是由多个QTL位点决定的。与产量有关的农艺性状的一些QTL集中在几个连锁群上。 相似文献
20.
Genetic Analysis and Molecular Mapping of a Novel Gene Conferring Resistance to Rice Stripe Virus 总被引:1,自引:0,他引:1
Feng Zhao Zhijun Cai Tiezhu Hu Haigen Yao Li Wang Na Dong Bin Wang Zhengang Ru Wenxue Zhai 《Plant Molecular Biology Reporter》2010,28(3):512-518
Rice stripe virus (RSV) is one of the most damaging diseases affecting rice in East Asia. Rice variety 502 is highly resistant
to RSV, while variety 5112 is extremely susceptible. Field statistical data revealed that all “502 × 5112” F1 individuals were resistant to RSV and the ratio of resistant to susceptible plants was 3:1 in the F2 population and 1:1 in the BC1F1 population. These results indicated that a dominant gene, designated RSV1, controlled the resistance. Simple sequence repeat (SSR) analysis was subsequently carried out in an F2 population. Sixty SSR markers evenly distributed on the 12 rice chromosomes were screened and tested. Two markers, RM229
and RM206, showed linkage with RSV1. Based on this result, six SSR markers flanking RM229 and RM206 were further selected and tested. Results indicated that
SSR markers RM457 and RM473E were linked to RSV1 with a genetic distance of 4.5 and 5.0 cM, respectively. All of the four SSR markers (RM229, RM473E, RM457 and RM206) linked
to RSV1 were all located on chromosome 11, therefore RSV1 should be located on chromosome 11 also. In order to find some new markers more closely linked to the RSV1 gene, sequence-related amplified polymorphism (SRAP) analysis was performed. A total of 30 SRAP primer-pairs were analyzed,
and one marker SR1 showed linkage with RSV1 at a genetic distance of 2.9 cM. Finally, RSV1 gene was mapped on chromosome 11 between SSR markers RM457 and SRAP marker SR1 with a genetic distance of 4.5 cM and 2.9 cM,
respectively. 相似文献