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1.
Swasti S. Swain Tapasi Tripathy Pradipta K. Mohapatra Pradeep K. Chand 《In vitro cellular & developmental biology. Plant》2010,46(2):134-141
In vitro regeneration of black nightshade (Solanum nigrum L.) plants was achieved through callus-mediated shoot organogenesis followed by 30 d indoor ex vitro adaptation to nutritional stress under environmental ambience and thereafter 6-d outdoor acclimatization in pots prior to
field establishment. Relevant physiological parameters including pigment content, chlorophyll a fluorescence, net photosynthetic rate (P
N), transpiration rate (E), and stomatal conductance (g
s) of in vitro-regenerated plants were investigated during the course of ex vitro adaptation. During the first 4 d of indoor transplantation to potting substrate, there was a marginal reduction in the leaf
chlorophyll and carotenoid contents but P
N and E were strongly reduced. The stomatal conductance and E/P
N ratio were significantly higher in plants up to 20 d of indoor adaptation than those of comparable age grown naturally from
seeds. The shape of the OJIP fluorescence transient varied significantly with acclimatization, and the maximum change was
observed at 2.0 ms. The 2.0 ms variable fluorescence (V
j), 30 ms relative fluorescence (M
0), photon trapping probability (TR0/Abs), and photosystem II (PSII) trapping rate (TR0/RC) showed initial disturbance and subsequent stabilization during 30 d of indoor acclimatization. Energy dissipation (DI0/RC) and electron transport probability (ET0/TR0) showed an initial phase of increase during the 4 d after plants were transplanted outdoors. During the 6-d outdoor acclimatization
after transfer of plants to soil, no significant change in total chlorophylls and carotenoids, E, and g
s were observed, but P
N improved after reduction on the first d. The OJIP-derived parameters experienced change on the first d but were stabilized
quickly thereafter. There was no significant difference between outdoor acclimatized plants and those of the seed-grown plants
of comparable age with respect to photosynthetic and fluorescence parameters. Direct transfer of plants without indoor acclimatization,
however, showed a completely different trend with respect to P
N, E, and OJIP fluorescence transients. The bearing of this study on optimizing micropropagation is discussed. 相似文献
2.
There are three most important bacterial causative agents of serious infections that could be misused for warfare purposes:
Bacillus anthracis (the causative agent of anthrax) is the most frequently mentioned one; however, Fracisella tularensis (causing tularemia) and Yersinia pestis (the causative agent of plague) are further bacterial agents enlisted by Centers for Disease Control and Prevention into the category A of potential biological weapons. This review intends to summarize basic information about these bacterial
agents. Military aspects of their pathogenesis and the detection techniques suitable for field use are discussed. 相似文献
3.
4.
N. Yu. Markelova 《Microbiology》2010,79(6):777-779
Interaction of Bdellovibrio bacteriovorus 100NCJB with bacteria Campylobacter jejuni (strains 1, 2, 3, 4, and 5) and Helicobacter pylori, strain TX30a, was confirmed. The results indicate that lytic activity of bdellovibrios both in liquid media and cells attached
to a surface was observed. The potential use of the antimicrobial activity of predatory bacteria for environmental bioprotection
and public health is discussed. 相似文献
5.
Andrés Gonzalo Gutiérrez Silvio James Carabalí Olga Ximena Giraldo César Pompilio Martínez Fernando Correa Gustavo Prado Joe Tohme Mathias Lorieux 《BMC plant biology》2010,10(1):6
Background
Developing new population types based on interspecific introgressions has been suggested by several authors to facilitate the discovery of novel allelic sources for traits of agronomic importance. Chromosome segment substitution lines from interspecific crosses represent a powerful and useful genetic resource for QTL detection and breeding programs. 相似文献6.
O. V. Golovanova V. I. Konenkov A. V. Shevchenko M. V. Smolnikova 《Russian Journal of Genetics》2009,45(8):981-986
Based on population analysis of the DRB1, DQA1, DQB1 and TNFA allele frequency distribution patterns, regional features of immunogenetic structure of the population of West Siberia were
investigated. Statistically significant linkage disequilibrium within the HLA class II region, as well as between the TNFA and DRB1, DQA1, and DQB1 was demonstrated. Population frequency distribution patterns of two- and multilocus haplotypes were examined. 相似文献
7.
8.
Wang Minqin Zhao Junsheng Peng Zhenying Guo Wei Wang Yun Wang Le Xia Guangmin 《Plant Cell, Tissue and Organ Culture》2008,92(2):121-130
In order to investigate chromosome elimination in symmetric somatic hybridization between Bupleurum scorzonerifolium and Arabidopsis thaliana, protoplasts were isolated from suspension cultures of both A. thaliana and B. scorzonerifolium parents. Biparental protoplasts were mixed at a rate of 1.5:1 and fused with PEG-method. After protoplast fusion, the products
were cultured in the P5 liquid medium for microcallus formation. Single cell lines formed from microcalli after subculturing
on the MB1 (Xia and Chen, Plant Sci 120:197–203, 1996) solid medium. The putative somatic hybrid cell lines were identified
by cytological and molecular analysis. Of the 132 somatic cell lines generated, 16 were identified as somatic hybrids, with
the phenotypes resembled B. scorzonerifolium parent. These hybrids showed a complete set of B. scorzonerifolium chromosome and 0–2 small chromosome(s) of A. thaliana. A few of them showed nuclear and cytoplasmic SSR fragments of A. thaliana. These hybrid cell lines could differentiate to green spots, buds/leaves through complementation of regeneration ability.
The chromosomes elimination of A. thaliana was discussed.
Wang Minqin and Zhao Junsheng contributed equally to the work. 相似文献
9.
To overexpress the chitosanase gene (csn) in F. solani, a vector based on pCAMBIA 1300 was constructed. The csn gene, which is under control of the Aspergillus nidulans
gpdA promoter and A. nidulans trpC terminator, was introduced back into the F. solani genome by Agrobacterium tumefaciens-mediated transformation, and the herbicide-resistance gene bar from Streptomyces hygroscopicus was used as the selection marker. Transformants which showed a significant increase in chitosanase production (~2.1-fold
than control) were obtained. Southern blot analysis indicated that most transformants had a single-copy T-DNA integration. 相似文献
10.
The intron sequence of chloroplast rpS16 and the secondary structure of its pre-mRNA were characterized for the first time in 26 Allium sativum accessions of different ecologo-geographical origins and seven related Allium species. The boundaries and main stem-loop consensus sequences were identified for all six domains of the intron. Polymorphism
was estimated for the total intron and its regions. The structural regions of the rpS16 intron proved to be heterogeneous for mutation rate and spectrum. Mutations were most abundant in domains II and IV, and
transition predominated in domains I, III, V, and VI. In addition to structural elements and motifs typical for group IIB
introns, several Allium-specific micro- and macrostructural mutations were revealed. A 290-bp deletion involving domains III and IV and part of domain
V was observed in A. altaicum, A. fistulosum, and A. schoenoprasum. Several indels and nucleotide substitutions were found to cause a deviation of the pre-mRNA secondary structure from the
consensus model of group II introns. 相似文献
11.
Hiroyoshi Kubo 《Mycoscience》2009,50(5):400-406
Pilobolus crystallinus shows unique photoresponses at various growing stages. cDNAs for putative photoreceptors were cloned from this fungus. Three
genes named pcmada1, pcmada2, and pcmada3 were identified from the PCR fragments, and amplified with degenerated primers for the LOV domain, which is conserved in
many blue-light receptors. Deduced amino acid sequences for PCMADA1, PCMADA2, and PCMADA3 had one light-oxygen-voltage (LOV)-sensing
and two PER-ARNT-SIM (PAS) domains. A zinc finger DNA-binding motif was conserved in the C-terminals of PCMADA1 and PCMADA3.
However, PCMADA2 lacked the zinc finger motif. Expression of pcmada1 was suppressed by blue light whereas that of pcmada3 was promoted by blue-light irradiation. 相似文献
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13.
Debabrata Sarkar Sushruti Sharma Poonam Chandel Suman Kumar Pandey 《Plant Growth Regulation》2010,61(1):109-117
Gametoclonal variation that occurs in gametic cells in culture and is recovered in their regenerated derivatives has not been
reported in potato (Solanum tuberosum L.). Based on a set of 24 differentiating phenotypic traits, canonical variates analysis genetically distinguished the androgenic
(di)haploid (2n = 2x = 24) D4 from its tetraploid (2n = 4x = 48) anther-derived sibs and anther donor JTH/C-107. Nuclear microsatellite analysis over six polymorphic loci indicated
that meiotic rearrangements and mutant alleles were primarily associated with the release of gametoclonal variation. The incidence
of null alleles in D4 at the loci STACCAS3 and STM0031 was also indicative of mutations occurring within the priming sequence. Microsatellite results were supported by random amplified
polymorphic DNA (RAPD) assays that characterized a total of 567 loci (bins) representing 4,258 amplified fragments. Sixty-five
new RAPDs that were absent in the anther donor and in either of its parents, viz., S. phureja Juz. & Buk. IVP-35 and S. tuberosum cv. Kufri Jyoti were present in D4, indicating the occurrence of extensive recombinational events. The results have been
discussed in the context of microsatellite null alleles providing the most conclusive evidence for gametoclonal variation. 相似文献
14.
I. Rosario G. Soro S. Déniz O. Ferrer F. Acosta D. Padilla B. Acosta 《Mycopathologia》2010,169(4):315-319
Columba livia is an important reservoir and carrier of Cryptococcus neoformans, Cryptococcus uniguttulatus, Cryptococcus laurentii and Cryptococcus albidus. Upper digestive tract of this species is also known as a habitat for Cryptococcus neoformans. Given the increasing clinical interest of this microorganism, 331 swabs from crop and 174 dropping samples from pigeon lofts
in Grand Canary Island have been studied. The obtained results show an extensive presence samples 81 positive (24.47%) of
Cryptococcus spp. in analysed crops: 32 (9.66%) for C. neoformans, 24 (7.2%) for C. uniguttulatus, 23 (6.9%) for C. albidus and 2 (0.6%) for C. laurentii. In the same way, Cryptococcus spp was also isolated in 82 (47.13%), dropping samples: C. neoformans in 59 (33.9%), C. uniguttulatus, in 9 (5.17%), C. laurentii in 8 (4.59%) and C. albidus in 6 (3.44%) of the investigated samples, respectively. The cryptococcosis produced by species of cryptococci other than C. neoformans has become more important during the last decade, supporting the study on the role of pigeon in the epidemiology of this
disease. 相似文献
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16.
Sachiko Funayama-Noguchi Ko Noguchi Chie Yoshida Masayoshi Kawaguchi 《Journal of plant research》2011,124(1):155-163
Genes of CLE (CLAVATA3/ESR-related) family encode peptide ligands that regulate plant development in response to external stimuli such as rhizobial
infection and the nitrate application as well as various internal stimuli. To investigate whether LjCLE gene(s) may involve in plant response to inorganic phosphate (Pi), we analyzed Pi responses of 39 LjCLE genes in hydroponically grown Lotus japonicus plants (ecotype Miyakojima ‘MG-20’). Two LjCLE genes, LjCLE19 and 20, were up-regulated specifically and greatly in roots of L. japonicus by Pi addition to the hydroponic solution. When the external Pi level increased, expressions of LjCLE19 and 20 increased before the increase in the Pi content in plants. On the other hand, when the external Pi level decreased, the Pi
content in plants decreased first, then expression levels of LjCLE19 and 20 decreased. Based on our results, we discuss the relationship between LjCLE19 and 20 and the tissue Pi levels in plants. This is the first report showing induction of specific CLE genes by phosphate. 相似文献
17.
Following a re-examination of the material treated under Barleria brevispina (Fiori) Hedrén in the recent Flora of Somalia account of the Acanthaceae, it is concluded that two distinct species are involved and Barleria compacta Malombe & I. Darbysh. is described here from north-eastern Somalia. Its affinities and conservation status are discussed. 相似文献
18.
Gaye Öngen Gaye Güngör Bahar Kanberoglu 《World journal of microbiology & biotechnology》2007,23(4):519-524
Aspergillus section Nigri strains Aspergillus aculeatus Ege-K 258, A.
foeditus var. pallidus Ege-K156, A. niger Ege-K 4 and A. tubingensis Ege-K 265 were used to treat olive mill wastewater (OMW) in an investigation aimed at exploring their dephenolisation and
decolourisation ability and, consequently, the economic feasibility of using any or all of these strains in a pre-treatment
step in the processing of OMW. Of these strains A. tubingensis Ege-K 265 resulted in an 80% decolourisation of twofold-diluted OMW and a 30% decolourisation of undiluted OMW; in addition,
it was able to remove approximately 30% of all phenolic compounds in both twofold-diluted and undiluted OMW. We conclude that
A. tubingensis Ege-K 265 could be effectively used in the pre-treatment step of a combined aerobic-anaerobic process to solve the environmental
problems caused by OMW in Mediterranean countries. 相似文献
19.
Nanobodies (or VHHs) are single-domain antigen-binding fragments derived from Camelid heavy chain-only antibodies. Their small size, monomeric behaviour, high stability and solubility, and ability to bind epitopes
not accessible to conventional antibodies make them especially suitable for many therapeutic and biotechnological applications.
Here we describe high-level expression, in Nicotiana benthamiana, of three versions of an anti-hen egg white lysozyme (HEWL) nanobody which include the original VHH from an immunized library
(cAbLys3), a codon-optimized derivative, and a codon-optimized hybrid nanobody comprising the CDRs of cAbLys3 grafted onto
an alternative ‘universal’ nanobody framework. His6- and StrepII-tagged derivatives of each nanobody were targeted for accumulation
in the cytoplasm, chloroplast and apoplast using different pre-sequences. When targeted to the apoplast, intact functional
nanobodies accumulated at an exceptionally high level (up to 30% total leaf protein), demonstrating the great potential of
plants as a nanobody production system. 相似文献
20.
The life cycle and developmental stages of Aylax hypecoi (Trotter, 1913, Hymenoptera: Cynipidae: Aylacini) were studied in detail. Aylax hypecoi is known to induce galls in fruits of two Hypecoum species — H. imberbe and H. geslini (Papaveraceae) and the larva develops in host plant fruits. The morphology and development of egg, larva and pupa were investigated, which
has previously not been done. The shape and size of terminal-instar larvae and associated galls are sex-specific. Overwintering
stage, adult emergence and flying periods, and egg productivity were studied also. 相似文献