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Differential transcription of Xenopus oocyte and somatic-type 5 S genes in a Xenopus oocyte extract 总被引:10,自引:0,他引:10
L Millstein P Eversole-Cire J Blanco J M Gottesfeld 《The Journal of biological chemistry》1987,262(35):17100-17110
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Assembly of transcriptionally active chromatin in Xenopus oocytes requires specific DNA binding factors 总被引:22,自引:0,他引:22
Active minichromosomes assembled on injected 5S RNA gene clones are stable in Xenopus oocytes; endogenous 5S DNA specific factor(s) are required for their assembly. When somatic-type and oocyte-type 5S RNA gene clones are coinjected, the somatic genes are assembled into active minichromosomes, while most of the oocyte genes are assembled into inactive ones. The differential 5S RNA gene expression, which mimics that in somatic cells, appears to result from titration of 5S DNA specific factor(s) by the competing somatic 5S DNA, followed by histone mediated assembly of inactive chromatin on the oocyte 5S DNA. Stable minichromosomes are also assembled on a cloned histone H4 gene; again, intragenic DNA rearrangements affect the efficiency of assembly of active chromatin and differential gene expression occurs after coinjection of two or more H4 DNA constructs. We suggest that the H4 DNA molecules also compete for limiting quantities of specific DNA binding factor(s) required for the assembly of active H4 gene chromatin. 相似文献
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Transcription fraction TFIIIC can regulate differential Xenopus 5S RNA gene transcription in vitro 总被引:30,自引:4,他引:26
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![点击此处可从《The EMBO journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
A P Wolffe 《The EMBO journal》1988,7(4):1071-1079
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Xenopus 5S RNA genes exhibit a pattern of differential expression during development in which some members (oocyte-type) are transcribed only in oocytes, while others (somatic-type) are expressed in both oocytes and somatic cells. Using cloned DNA probes specific for each gene type, we determined the positions of these genes on Xenopus metaphase chromosomes by in situ hybridization. Somatic-type 5S genes in both X. laevis and X. borealis are located at the distal end of the long arm of only one chromosome (number 9). The oocyte-type 5S RNA genes are found at the distal ends of the long arms of most Xenopus chromosomes, including chromosome 9. Thus, large scale differences in chromosomal location cannot explain the selective expression of these genes, as suggested previously. 相似文献
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DNA sequence-directed nucleosome reconstitution on 5S RNA genes of Xenopus laevis. 总被引:14,自引:3,他引:11
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![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
J M Gottesfeld 《Molecular and cellular biology》1987,7(5):1612-1622
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