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1.
Judy A. Garner 《Journal of neurochemistry》1990,54(5):1700-1708
Rises in intracellular calcium cause several events of physiological significance, including the regulated release of neuronal transmitters. In this study, the effects of divalent cations on the structural organization of cytomatrix in presynaptic terminals was examined. [35S]Methionine-radiolabeled guinea pig retinal ganglion cell cytomatrix proteins were axonally transported [in slow component b (SCb) of axonal transport] to the neuron terminals in the superior colliculus. When the peak of radiolabeled cytomatrix proteins reached the terminals, synaptosomes containing the radiolabeled cytomatrix proteins were prepared. Approximately 40% of each SCb protein was soluble after hypoosmotic lysis of the radiolabeled synaptosomes in the presence of divalent cation chelators. Lysis of synaptosomes in the presence of calcium ions over a range of concentrations, however, caused a dramatic decrease in solubility of the presynaptic SCb proteins. The cytoplasmic effects may result from a calcium-dependent condensation of cytoplasm around presynaptic terminal membrane systems. There are two major presynaptic SCb proteins (at 60 and 35 kDa), that exhibited exceptional behavior: they remained as soluble in the presence of calcium as under control conditions, suggesting that they were relatively unaffected by the mechanism causing the decrease in SCb protein solubility. Also examined were the effects of other alkaline earth and transition metal divalent cations on the presynaptic SCb proteins. 相似文献
2.
Studies on the transport kinetics and the posttranslational modification of synapsin I in mouse retinal ganglion cells were
performed to obtain an insight into the possible factors involved in forming the structural and functional differences between
the axon and its terminals. Synapsin I, a neuronal phosphoprotein associated with small synaptic vesicles and cytoskeletal
elements at the presynaptic terminals, is thought to be involved in modulating neurotransmitter release. The state of phosphorylation
of synapsin I in vitro regulates its interaction with both synaptic vesicles and cytoskeletal components, including microtubules
and microfilaments. Here we present the first evidence that in the mouse retinal ganglion cells most synapsin I is transported
down the axon, together with the cytomatrix proteins, at the same rate as the slow component b of axonal transport, and is
phosphorylated at both the head and tail regions. In addition, our data suggest that, after synapsin I has reached the nerve
endings, the relative proportions of variously phosphorylated synapsin I molecules change, and that these changes lead to
a decrease in the overall content of phosphorus. These results are consistent with the hypothesis that, in vivo, the phosphorylation
of synapsin I along the axon prevents the formation of a dense network that could impair organelle movement. On the other
hand, the dephosphorylation of synapsin I at the nerve endings may regulate the clustering of small synaptic vesicles and
modulate neurotransmitter release by controlling the availability of small synaptic vesicles for exocytosis. 相似文献
3.
TRANSPORT AND TURNOVER OF NEUROHYPOPHYSIAL PROTEINS OF THE RAT 总被引:2,自引:0,他引:2
Axonal transport and turnover rate of proteins in the supraoptico-neurohypo-physial tract were studied after injection of 35S cysteine into the region of the supraoptic nucleus. The proximo-distal migration of labelled proteins from the nerve cell bodies to the axon terminals in the neurohypophysis was followed by measuring the radioactivity of neurohypophysial proteins at various time intervals (4 h to 30 days) after isotope injection. A rapidly transported phase of proteins with a minimal transport rate of approximately 60 mm/day was demonstrated. An accumulation of protein-bound radioactivity was also observed in the neural lobe at 9 days after isotope injection, representing slowly transported proteins (0-5 mm/day). In addition, an intermediate phase of axonal transport (1-5 mm/day) was found. Fractionation of neurohypophysial proteins by polyacrylamide gel disc electrophoresis revealed that a predominating portion of the radioactivity was recovered in a single protein component (fraction A) at 4 h as well as at 30 days after isotope injection. This protein component was shown to be a constituent both of the rapid and the slow phase of axonal transport. With time an increasing amount of radioactivity was found in another protein component (fraction B), which reached a maximum at 14 days after injection and then remained fairly constant up to 30 days. When the turnover rates of neurohypophysial proteins were estimated, a half-life of 1-2 days and 8 days was calculated for the rapidly and slowly transported proteins, respectively. 相似文献
4.
M. Vitadello G. Filliatreau J. L. Dupont† R. Hassig A. Gorio L. Di Giamberardino 《Journal of neurochemistry》1985,45(3):860-868
Polypeptides in the motor axons of the sciatic nerve in 120-day-old normal and diabetic mice C57BL/Ks (db/db) were labeled by injection of [35S]methionine into the ventral horn of the spinal cord. At 8, 15, and 25 days after the injection, the distribution of radiolabeled polypeptides along the sciatic nerve was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Four major radiolabeled polypeptides, tentatively identified as actin, tubulin, and the two lightest subunits of the neurofilament triplet, were studied in both diabetic and control mice. In the diabetic animals, the two polypeptides identified as actin and tubulin showed a reduction of average velocity of migration along the sciatic nerve, resulting in a higher fraction of radioactivity in the proximal part of the sciatic nerve, whereas the front of radioactivity (advancing at maximal velocity) moved at a normal rate. In contrast, both the average and maximal velocities of the two neurofilament subunits were slower in the diabetic mice than in the control mice. These results indicate that the axonal transport of the cytoskeletal proteins is differentially affected in the course of diabetic neuropathy, and may suggest that the impairment concerns mainly the proteins carried by the slowest component of axonal transport. 相似文献
5.
Effects of single and repeated doses of acrylamide on fast and slow axonal transport of radio labeled proteins following the injection of L-[4,5-3H] leucine have been studied in the optic system of male Sprague-Dawley rats. A single dose of acrylamide (100 mg/kg) had no effect, but higher concentrations (200–300 mg/kg) altered the distribution of fast axonally transported materials in optic nerves and optic tracts. Repeated doses of acrylamide (30 mg/kg/day, 5 days per week for 4 weeks) produced degeneration of tibial nerves but spared optic nerves and optic tracts. Fast axonal transport rate in optic axons was reduced by 50% (reduced to 4 mm/h from 8 mm/h) in acrylamide treated animals. Acrylamide also slowed the velocity of slow axonal transport of labeled proteins in optic axons to 1.0 mm per day from 1.3 mm per day. Since acrylamide impaired the rate of both fast and slow axonal transport in the absence of overt morphological damage, it can be concluded that deficit in axonal transport is an important factor in the pathogenesis of axonal degeneration in acrylamide neuropathy. 相似文献
6.
SYNTHESIS, MIGRATION AND TURNOVER OF PROTEIN IN RETINAL GANGLION CELLS 总被引:21,自引:7,他引:14
Abstract— The synthesis, migration and turnover of proteins in retinal ganglion cells of the adult rabbit was studied after intraocular injections of [3 H]leucine. It was shown that the isotope was rapidly incorporated into proteins of the retina and some of the proteins were subsequently transported out into the axons of the retinal ganglion cells down to the terminals. This intra-axonal transport of protein occurred at four different velocities; 150, 40, 6-12 and 2 mm/day respectively. The two most rapidly migrating phases of axonal transport were predominantly associated with light particulate fractions and had a relatively rapid turnover in the nerve terminals in the lateral geniculate body. The third phase of axonal transport which had a rate of 6-12 mm/day was possibly associated with the migration of mitochondria. The most slowly migrating proteins in the axon which moved at an average rate of 2 mm/day carried predominantly soluble proteins down to the nerve terminals. A minor part of this phase was metabolized locally in the axon with a half-life of about 14 days. When this slowly migrating phase had reached the nerve terminals in the lateral geniculate body, it was degraded with a half-life of 9-6 days. The different phases of axonal transport were of different magnitudes. As measured from the maximal amount of radioactivity present in the nerve terminals the relative amounts of radioactivity of the four phases were: 1,1 -8,1 -5 and 8-5. 相似文献
7.
John J. Bray Paul Fernyhough James R. Bamburg Dennis Bray 《Journal of neurochemistry》1992,58(6):2081-2087
We examined the low molecular weight proteins transported with actin in the chicken sciatic nerve after injection of [35S]methionine into the lumbar spinal cord. A prominent component of slow axonal transport with apparent molecular mass 19 kDa comigrated on two-dimensional gels with chicken actin depolymerizing factor (ADF), previously shown to be a major actin-binding protein in brain. There was comparatively little radioactivity associated with the actin monomer sequestering proteins, profilin or cofilin, and examination of the rapid component of axonal transport failed to reveal appreciable quantities of actin, ADF, profilin, or cofilin. These results show that both actin and ADF are carried by slow axonal transport and raise the possibility that actin travels within the axon in an unpolymerized form in a complex with ADF. 相似文献
8.
Abstract: While studying the delivery of cytoplasmic proteins to the presynaptic terminals of CNS neurons, we discovered unique characteristics of one protein (p118) conveyed in slow component b (SCb) of axonal transport, the large group of proteins representing the cytoplasmic matrix. Alone among the SCb group, p118 coisolated with the synaptic junctional complex on biochemical fractionation of the radiolabeled synaptic regions. Purification and amino acid sequencing of this protein revealed it is most likely the guinea pig form of type I (brain) hexokinase (ATP: d -hexose 6-phosphotransferase, EC 2.7.1.1). Further biochemical treatments were consistent with this identity. The majority of type I brain hexokinase has been thought to be associated primarily with membranes, in particular the mitochondrial outer membrane. We found that the majority of type I hexokinase is transported toward the terminals at a rate at least 10 times slower than that exhibited by the maximal or average rate of mitochondria. This suggests that, in the axon, the enzyme exhibits transient or dynamic interactions with mitochondria that are moving more rapidly. It is not clear whether hexokinase binds exclusively to mitochondria, or also exhibits association with nonmitochondrial membranes. The unexpected enrichment of hexokinase during synaptic junctional complex purification may result from its strong association with the presynaptic membrane portion of the synapse. 相似文献
9.
Reversal of the direction (turnaround) of orthograde axonal transport of dopamine-beta-hydroxylase (DBH) activity was studied at a ligature placed on rat sciatic nerve. DBH was allowed to accumulate at a ligature in vivo for selected intervals, at which time a second ligature was placed proximal to the first and turnaround transport measured just distal to the second tie after incubation in vivo or in vitro. Orthograde accumulation of DBH activity proximal to a ligature peaked at 2 days, and then rapidly decreased as a result of turnaround transport and injury-induced reduction of orthograde transport. Destruction of postganglionic sympathetic axon terminals in vivo with 6 hydroxydopamine resulted in a decrease in orthograde transport similar to that seen after axotomy and turnaround at or proximal to the site of chemical injury. Turnaround transport of DBH in vitro was blocked by incubation in the cold and in the presence of NaCN and vinblastine. Orthograde transport of DBH appeared to reverse direction within a few millimeters of a ligature. 相似文献
10.
Anterograde Components of Axonal Transport in Motor and Sensory Nerves in Experimental 2,5-Hexanedione Neuropathy 总被引:2,自引:0,他引:2
Anterograde slow and fast axonal transport was examined in rats intoxicated with 2,5-hexanedione (1 g/kg/week) for 8 weeks. Distribution of radioactivity was measured in 3-mm segments of the sciatic nerve after labelling of proteins with [35S]methionine or [3H]leucine and glycoproteins with [3H]fucose. The axonal transport of the anterograde slow components was examined after 25 (SCa) and 10 days (SCb), in motor and sensory nerves. SCa showed an increased transport velocity in motor (1.25 +/- 0.08 mm/day versus 1.01 +/- 0.05 mm/day) and in sensory nerves (1.21 +/- 0.13 mm/day versus 1.06 +/- 0.07 mm/day). The relative amount of labelled protein in the SCa wave in both fiber systems was also increased. SCb showed unchanged transport velocity in motor as well as in sensory nerves, whereas the amount of label was decreased in the motor system. Anterograde fast transport in motor nerves was examined after intervals of 3 and 5 h, whereas intervals of 2 and 4 h were used for sensory nerves. Velocities and amounts of labelled proteins of the anterograde fast component remained normal. We suggest that the increase in protein transport in SCa reflects axonal regeneration. 相似文献
11.
Ralph A. Nixon Susan E. Lewis Marc Mercken Ram K. Sihag 《Neurochemical research》1994,19(11):1445-1453
Newly synthesized neurofilament proteins become highly phosphorylated within axons. Within 2 days after intravitreously injecting normal adult mice with [32P]orthophosphate, we observed that neurofilaments along the entire length of optic axons were radiolabeled by a soluble32P-carrier that was axonally transported faster than neurofilaments.32P-incorporation into neurofilament proteins synthesized at the time of injection was comparatively low and minimally influenced the labeling pattern along axons.32P-incorporation into axonal neurofilaments was considerably higher in the middle region of the optic axons. This characteristic non-uniform distribution of radiolabel remained nearly unchanged for at least 22 days. During this interval, less than 10% of the total32P-labeled neurofilaments redistributed from the optic nerve to the optic tract. By contrast, newly synthesized neurofilaments were selectively pulse-labeled in ganglion cell bodies by intravitreous injection of [35S]methionine and about 60% of this pool translocated by slow axoplasmic transport to the optic tract during the same time interval. These findings indicate that the steady-state or resident pool of neurofilaments in axons is not identical to the newly synthesized neurofilament pool, the major portion of which moves at the slowest rate of axoplasmic transport. Taken together with earlier studies, these results support the idea that, depending in part on their phosphorylation state, transported neurofilaments can interact for short or very long periods with a stationary but dynamic neurofilament lattice in axons.Special issue dedicated to Dr. Sidney Ochs. 相似文献
12.
Sandra L. Tanner Elaine E. Storm †‡ George D. Bittner 《Journal of neurochemistry》1995,64(4):1491-1501
Abstract: Using video-enhanced microscopy and a pulse-radiolabeling paradigm, we show that proteins synthesized in the medial giant axon cell body of the crayfish ( Procambarus clarkii ) are delivered to the axon via fast (∼62 mm/day) and slow (∼0.8 mm/day) transport components. These data confirm that the medial giant axon cell body provides protein to the axon in a manner similar to that reported for mammalian axons. Unlike mammalian axons, the distal (anucleate) portion of a medial giant axon remains intact and functional for >7 months after severance. This axonal viability persists long after fast transport has ceased and after the slow wave front of radiolabeled protein has reached the terminals. These data are consistent with the hypothesis that another source (i.e., local glial cells) provides a significant amount of protein to supplement that delivered to the medial giant axon by its cell body. 相似文献
13.
R. R. Islamov O. V. Tyapkina A. A. Eremeev G. F. Shaymardanova E. A. Chakkaeva I. B. Kozlovskaya E. E. Nikolsky 《Biophysics》2010,55(5):745-749
Spontaneous quantal neurotransmitter release and its modulation was studied on neuromuscular preparations of rat soleus from
intact animals and from animals in which colchicine had been applied to the sciatic nerve to block the axonal transport. After
six days of colchicine application, neither the spontaneous quantal secretion nor its reaction to potassium-induced membrane
depolarization or to activation of the presynaptic acetylcholine receptors with carbachol were disturbed in any way. Keeping
in mind the relatively short half-life of proteins that take part in exocytosis and its regulation, it may be concluded that
the functioning of the terminal neurosecretory apparatus does not depend on the state of axonal transport. These data are
consistent with the earlier hypothesis that some proteins performing their functions in nerve terminals are synthesized directly
at the site of their operation, rather than in the perikaryon as traditionally assumed. 相似文献
14.
Roland G. Mills Laurie S. Minamide Aidong Yuan James R. Bamburg John J. Bray 《Journal of neurochemistry》1996,67(3):1225-1234
Abstract: We examined the axonal transport of actin and its monomer binding proteins, actin depolymerizing factor, cofilin, and profilin, in the chicken sciatic nerve following injection of [35 S]methionine into the lumbar spinal cord. At intervals up to 20 days after injection, nerves were cut into 1-cm segments and separated into Triton X-100-soluble and particulate fractions. Actin and its binding proteins were then isolated by affinity chromatography on DNase I-Sepharose and by one- and two-dimensional polyacrylamide gel electrophoresis. Fluorographic analysis showed that the specific activity of soluble actin was two to three times that of its particulate form and that soluble actin, cofilin, actin depolymerizing factor, and profilin were transported at similar rates in slow component b of axonal flow. Our data strongly support the view that the mobile form of actin in slow transport is soluble and that a substantial amount of this actin may travel as a complex with actin depolymerizing factor, cofilin, and profilin. Along labeled nerves the specific activity of the unphosphorylated form of actin depolymerizing factor, which binds actin, was not significantly different from that of its "inactive" phosphorylated form. This constancy in specific activity suggests that continuous inactivation and reactivation of actin depolymerizing factor occur during transport, which could contribute to the exchange of soluble actin with the filamentous actin pool. 相似文献
15.
James K. Wamsley 《Molecular neurobiology》1992,6(2-3):313-321
The axonal transport of neurotransmitter receptors is thought to be a common phenomenon in many neuronal systems. The “machinery”
for receptor (protein) “assembly” is found in the cell bodies of neurons and the “manufacture” of receptors takes place there.
These receptors are then “shipped” to their ultimate destinations by a transport process. This is an axonal transport mechanism
in the case of presynaptic receptors. Some form of transport process may also exist to send receptors out into the dendritic
arborizations of neurons, although the latter is more difficult to verify. Axonal transport has been demonstrated, in the
peripheral nervous systems, for many different neurotransmitter receptors. In the central nervous system, the results are
less clear, but indicate the presence of a transport mechanism for catecholamine, acetylcholine, and opiate sites. One important
component then, in the development of receptors, is the transportation to terminal membrane sites where they are ultimately
incorporated and available for interaction with neurotransmitters and drugs. 相似文献
16.
The composition of retrogradely transported axonal proteins was examined by acrylamide gel electrophoresis and gel autoradiography in the experimental neuropathy induced in rats by p-bromophenylacetylurea (BPAU). Protein composition was normal during the early phase of retrograde transport but showed significant abnormalities during a later phase. The early phase consisted of proteins collected distal to a mid-thigh ligature of sciatic nerve between 15 and 24 hours after injection of [35S] methionine into lumbar ventral horn of the spinal cord. In terms of their relative labeling and electrophoretic mobility, these proteins were almost identical in experimental and control rats. Most of the labeled protein bands were also identical in the later phase, collected between 24 and 48 hours, but there were some consistent omissions and additions. Present in controls but missing in BPAU treated rats were three bands at 42, 41, and 25 KDa. In contrast, 4 bands (63, 56, 50, 26 KDa) were more prominent in the experimental rats than in controls. We suspect abnormal post-translational modification or proteolysis of rapidly transported proteins in the terminal or preterminal portion of the neurons exposed to BPAU. This abnormality, in addition to a previously reported premature processing of transported organelles, may underlie the development of peripheral neuropathy. 相似文献
17.
Qian Qiao Lei Ma Wei Li Jin‐Wu Tsai Guang Yang Wen‐Biao Gan 《Developmental neurobiology》2016,76(3):252-261
Many lines of evidence indicate that postsynaptic dendritic spines are plastic during development and largely stable in adulthood. It remains unclear to what degree presynaptic axonal terminals undergo changes in the developing and mature cortex. In this study, we examined the formation and elimination of fluorescently‐labeled axonal boutons in the living mouse barrel cortex with transcranial two‐photon microscopy. We found that the turnover of axonal boutons was significantly higher in 3‐week‐old young mice than in adult mice (older than 3 months). There was a slight but significant net loss of axonal boutons in mice from 1 to 2 months of age. In both young and adult barrel cortex, axonal boutons existed for at least 1 week were less likely to be eliminated than those recently‐formed boutons. In adulthood, 80% of axonal boutons persisted over 12 months and enriched sensory experience caused a slight but not significant increase in the turnover of axonal boutons over 2–4 weeks. Thus, similar to postsynaptic dendritic spines, presynaptic axonal boutons show remarkable stability after development ends. This long‐term stability of synaptic connections is likely important for reliable sensory processing in the mature somatosensory cortex. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 252–261, 2016 相似文献
18.
《Neurochemistry international》1987,11(2):155-160
Labelled axonally transported proteins belonging to four different phases of transport in the retinal ganglion cells of the rabbit were used as substrates in order to study proteolytic degradation in axons and nerve terminals.Proteins of both rapidly and slowly transported phases of axonal transport were easily degraded in small intact pieces of the superior colliculus.Addition of the Ca-dependent neutral protease, calpain, to isolated soluble and membrane fractions from the superior colliculus resulted in an increased rate of degradation of axonally transported components. The effects of calpain was most marked toward components in phases II and V of axonal transport in this system (Karlsson and Sjöstrand, 1971; Willard and Hulebak, 1977). The latter phase contains slowly transported neurofilament and microtubular protein while the former one contains rapidly transported membrane proteins. 相似文献
19.
Paola Strocchi Jeffrey M. Gilbert Larry I. Benowitz D. Dahl Ellen R. Lewis 《Journal of neurochemistry》1984,43(2):349-357
High resolution 2DGE (two-dimensional gel electrophoresis) was used to characterize neuronal and glial proteins of the rat optic nerve, to examine the phases of intraaxonal transport with which the neuronal proteins are associated, and to identify the ribosomal populations on which these proteins are synthesized. Neuronal proteins synthesized in the retinal ganglion cells were identified by injecting the eye with L-[35S]methionine, followed by 2DGE analysis of fast and slow axonally transported proteins in particulate and soluble fractions. Proteins synthesized by the glial cells were labeled by incubating isolated optic nerves in the presence of L-[35S]methionine and then analyzed by 2DGE. A number of differences were seen between filamentous proteins of neurons and glia. Most strikingly, proteins in the alpha- and beta-tubulin region of the 2D gels of glial proteins were distinctly different than was observed for axonal proteins. As expected, neurons but not glia expressed neurofilament proteins, which appeared among the slow axonally transported proteins in the particulate fraction; significant amounts of the glial filamentous protein, GFA, were also labeled under these conditions, which may have been due to transfer of amino acids from the axon to the glial compartment. The fast axonally transported proteins contained relatively large amounts of high-molecular-weight acidic proteins, two of which were shown to comigrate (on 2DGE) with proteins synthesized by rat CNS rough microsomes; this finding suggests that rough endoplasmic reticulum may be a major site of synthesis for fast transported proteins. In contrast, the free polysome population was shown to synthesize the principal components of slow axonal transport, including tubulin subunits, actin, and neurofilament proteins. 相似文献
20.
The effects of heat stress on protein synthesis and fast axonal transport were examined in an in vitro bullfrog primary afferent neuron preparation. The magnitude of effect was determined for individual [35S]methionine-labelled protein species separated via two-dimensional gel electrophoresis. Elevation of temperature of the preparation from 18 degrees C to 33 degrees C caused a transient inhibition of synthesis of non-heat-shock proteins, whereas the synthesis of a 74,000-dalton protein increased to 927% of controls after 4 h. Similar prolonged stress conditions had no effect on the relative abundance of 36 individual, newly synthesized proteins undergoing fast axonal transport. A dramatic exception was represented by a 55,000-dalton glycoprotein whose fast transport was increased to 291% of control. The increase in transport of this protein during a time when synthesis and transport of other non-heat-shock proteins were not enhanced suggests that it may play a unique role in the early cellular events that mediate survival or thermotolerance in the neuron. 相似文献