共查询到20条相似文献,搜索用时 15 毫秒
1.
Chen X Lu J Zhang Y He J Guo X Tian G Jin L 《International journal of biological macromolecules》2008,43(3):252-256
The objective of this study was to evaluate the immunomodulatory effects of the Paecilomyces sinensis polysaccharides (PtP) on the activity of macrophages and human monocytes. A water-soluble polysaccharide, with estimated molecular weight of 2.04 × 104 Da, was isolated from P. sinensis. The results indicate that PtP can increase the activity of LDH and ACP in AMφ and PMφ of rats and human mononuclear cells, and enhance the pinocytic activity of macrophages and TNF-α production by human peripheral blood mononuclear cells (PBMC), suggesting that PtP had potent immunomodulatory properties and could be explored as a novel potential immunostimulants for the food and pharmaceutical purpose. 相似文献
2.
To screen stimulators from Chinese medicinal insects for mycelial growth and polysaccharides production of Ganoderma lucidum, G. lucidum was inoculated into the media with and without supplementation of medicinal insect extracts. The ethyl acetate extract of
Eupolyphaga sinensis at 55 mg l−1 lead to significant increase in both biomass and intracellular polysaccharides (IPS) concentration from 8.53 ± 0.41 to 14.16 ± 0.43
and 1.28 ± 0.09 to 2.13 ± 0.11 g l−1, respectively. In addition, the ethyl acetate extract of Catharsius molossus at 55 mg l−1 significantly enhanced extracellular polysaccharides (EPS) production; the EPS yield increased from 350.9 ± 14.1 to 475.1 ± 15.3 mg
l−1. There were no new components in the two types of polysaccharides obtained by the addition of the insect extracts. 相似文献
3.
The bioactive components of Ganoderma formosanum have not yet been characterized. We investigated the immunomodulatory activities of the extracellular polysaccharides produced
from a submerged mycelial culture of G. formosanum. The polysaccharides were mainly composed of d-mannose, d-galactose and d-glucose. After gel filtration chromatography, three polysaccharide fractions (PS-F1, PS-F2 and PS-F3) were purified. PS-F2
stimulated mouse RAW264.7 macrophages to produce TNF-α and nitric oxide, and enhanced the phagocytic activity of macrophages.
PS-F2 challenge in mice triggered an acute inflammatory response characterized by the recruitment of neutrophils and monocytes,
which protected mice from subsequent infection of Listeria monocytogenes. The results indicate that the heteropolysaccharides produced by G. formosanum can activate the innate immune response on macrophages. 相似文献
4.
This study examined the interaction of the poorly metastatic human melanoma cell line M4Be and the highly metastatic clone
4 derived from M4Be, with respect to fresh adherent leukocytes (AL) isolated from 17 different healthy blood donors. These
AL contained 80% (73%–93%) monocytes, 15% (6%–20%) B lymphocytes and 5% (1%–8%) T lymphocytes. The survival of these tumor
cells against the stress exerted by these AL was estimated with a clonogenic assay where isolated tumor cells were co-cultured
for 14 days in contact with AL and lipopolysaccharide (LPS). For a given blood donor, AL either stimulates or inhibits the
colony formation of the tumor cells (T) depending on the AL/T ratio, the AL activation status and the metastatic potential
of tumor cells. At low AL/T ratios (<10/1) in the presence of low (8 ng/ml) and trace (8 pg/ml) levels of LPS, hydrogen peroxide
(H2O2) release is significantly reduced, and tumor cells significantly increase their colony formation; the feeder effect of AL
is suggested to be due to low concentrations of soluble tumor necrosis factor-alpha (TNF-α). At high AL/T ratios (>10/1),
whatever the characteristics of the blood donor, clone 4 is significantly more sensitive than M4Be to AL activated with medium
containing low (8 ng/ml) or high (1,000 ng/ml) levels of LPS; this killing effect is suggested to be due to TNF-α, both soluble
and membrane-bound, but not to be due to release of H2O2. These data suggest that the regulatory role of AL, which remove the majority of human melanoma cells and stimulate the colony
formation of a small fraction of them, is partly due to TNF-α.
Received: 2 November 2000 / Accepted: 15 February 2001 相似文献
5.
Mohamed A. Haidara Mohamed D. Morsy Hesham A. Abdel-Razek Dimitri P. Mikhailidis Esma R. Isenovic 《Journal of physiology and biochemistry》2010,66(3):255-264
Septicemia leads to oxidative stress with overproduction of reactive-oxygen species (ROS) and consumption of endogenous antioxidant
enzymes. We tested a twofold hypothesis: (1) does oxidative stress (OxS) induced by sepsis acting alone or in concert with
augmented inflammatory processes contributes to sepsis-related vascular dysfunction, and, (2) whether ozone (O3) and l-canavanine (CAV) mitigate the negative impact of the aforementioned phenomena. We investigated the relative impact of treatment
with CAV and/or O3 on vascular OxS associated vascular functional changes in septicemic rats. For this study, 60 male Sprague–Dawley rats were
used and divided into six experimental groups (n = 10): control group (C), sham-operated (Sham), septicemic rats (S), S rats treated with CAV (100 mg/kg. i.p; S + CAV), S
rats treated with O3 (1.2 mg/kg, i.p.; S + O3) and S rats treated with both O3 and CAV (S + O3 + CAV). After 22 h, the mean arterial blood pressure (MAP), the aortic ring vascular reactivity to phenylephrine, abdominal
aortic blood flow (AABF), serum tumor necrosis factor-α (TNF-α) and plasma nitrite/nitrate (NOx) concentration were measured.
In addition, hepatic antioxidant enzyme activities sodium dismutase (SOD) and glutathione peroxidase (GSH-Px) were estimated.
Septicemia caused significant elevation of serum TNF-α (p < 0.001) and plasma NOx (p < 0.001) and significant (p < 0.001) reduction of AABF (p < 0.001), aortic vascular response to phenylephrine (p < 0.001), MAP (p < 0.001) and hepatic SOD and GSH-Px activity (p < 0.001) compared with the C group, while treatment with O3 and/or CAV induced significant amelioration of all those increases. Abnormalities were attenuated to a similar extent with
treatment with both O3 and CAV. These results suggested that concomitant administration of O3 and CAV alleviated the compromised vascular reactivity in septicemic conditions and prevent its progression into septic shock
compared with each alone. 相似文献
6.
7.
The lactone 6-pentyl-α-pyrone has a characteristic coconut aroma and is produced by Trichoderma species. A study on the fermentative production of 6-pentyl-α-pyrone in both surface and submerged conditions by Trichoderma harzianum was carried out. Maximum concentrations of 455 mg/l and 167 mg/l after 96 h and 48 h of fermentation in surface and submerged
conditions, respectively, were obtained without using any additional recovery operations. The resultant yields are higher
than those previously reported in the literature, which may be attributable to strain characteristics in combination with
the choice of fermentation conditions employed in the present study. Enough scope exists for further improvement in the yields
by optimizing the cultural and nutritional parameters.
Received: 13 July 1999 / Received revision: 19 November 1999 / Accepted: 19 November 1999 相似文献
8.
Filipendula ulmaria (L.) Maxim (meadowsweet) is a medicinal plant that is claimed to have several biological activities, including anti-tumor,
anti-carcinogenic, anti-oxidant, anti-coagulant, anti-ulcerogenic, anti-microbial, anti-arthritic, and immunomodulatory properties.
This report describes, for the first time, an efficient plant regeneration system for F. ulmaria via adventitious shoot development from leaf, petiole, and root explants cultured on Murashige and Skoog’s minimal organics
medium containing different concentrations of thidiazuron (TDZ), benzyladenine, and kinetin either alone or in combination
with different auxins. Relatively extensive/prolific shoot regeneration was observed in all three explant types with TDZ in
combination with indole-3-acetic acid (IAA). Gibberellic acid (GA3), TDZ, and IAA combinations were also tested. The best shoot proliferation was observed among root explants cultured on media
supplemented with 0.45 μM TDZ + 2.85 μM IAA + 1.44 μM GA3. Regenerated shoots were transferred to rooting media containing different concentrations of either IAA, indole-3-butyric
acid (IBA), naphthalene acetic acid, or 2,4-dichlorophenoxyacetic acid. Most shoots developed roots on medium with 2.46 μM
IBA. Rooted explants were transferred to vermiculite in Magenta containers for a 2-wk acclimatization period and then finally
to plastic pots containing potting soil. The plantlets in soil were kept in growth chambers for 2 wk before transferring to
greenhouse conditions. 相似文献
9.
Changes in the levels of antiapoptotic protein B-cell lymphoma 2 (Bcl-2) protein has been reported in murine and human tuberculosis.
We investigated the role of mitogen-activated protein kinase pathways in the production of Bcl-2 protein in THP-1 human monocytes
infected with Mycobacterium tuberculosis H37Rv and H37Ra. Analysis of phosphorylation profiles of mitogen-activated protein kinase kinase-1, extracellular-signal
regulated kinase 1/2, mitogen-activated protein kinase kinase 3/6, and p38 mitogen-activated protein kinase; B-cell lymphoma
2 kinetics; and tumor necrosis factor-α (TNF-α) secretion levels showed variation between the two strains. Mycobacterium tuberculosis H37Rv induced higher Bcl-2 and lower TNF-α levels, whereas H37Ra the reverse. The strains also differed in their usage of
CD14 and human leukocyte antigen-DR receptors in mediating extracellular-signal regulated kinase 1/2 and p38 mitogen-activated
protein kinase activation. Mycobacterium tuberculosis H37Rv- and H37Ra-induced Bcl-2 production was reduced by specific inhibitors of mitogen-activated protein kinase kinase-1
(PD98059) and p38 (SB203580), but increased by nuclear factor κB (NF-κB) inhibitor (BAY 11-7082). TNF-α production by both
strains was reduced in the presence of specific inhibitors of mitogen-activated protein kinase kinase-1 (PD98059), p38 (SB203580),
and NF-κB (BAY 11-7082). Furthermore, inhibition of NF-κB was accompanied by an increase in strain-induced extracellular-signal
regulated kinase 1/2 phosphorylation. Collectively, these results indicate for the first time that the production of Bcl-2
and TNF-α by M. tuberculosis H37Rv/H37Ra-infected THP-1 human monocytes is mediated through mitogen-activated protein kinases and NF-κB. 相似文献
10.
The main objective of this investigation was to examine physiological changes occurring within the olive flounder, Paralichthys olivaceus, in the presence of continuous stress factors. The experiment gave continuous stress, and each stress step is as follows:
water level reduction (S1), selection process (S2), confinement (S3), air exposure (S4), transportation (S5), and storing
in volume 0.5-t (S6) and 50.0-t (S7) rearing tanks for 24 h after transportation. The cortisol concentration showed a trend
to continuously rise in response to consecutive stress from transportation from 7.4 ± 0.6 ng/ml in an experimental opening.
The concentration showed the highest level, 25.3 ± 4.4 ng/ml, after confinement stress. Glucose concentration in S3 and S4
were increased significantly to 71.0 ± 13.0 and 78.7 ± 7.0 mg/dl, respectively (P < 0.05). Lactic acid was increased significantly from 0.5 ± 0.1 mM (S0) to 9.0 ± 1.2 mM (S4), but it did not recover until
24 h (7.0 ± 1.0 mM) after transportation (S6 and S7) (P < 0.05). Some contents displayed recovery within 24 h, but a longer time would be required for normal physical metabolism
to resume after fish experienced stress. Therefore, it would be endangering the survival of this species if the transport
were repeated within a 24-h period. 相似文献
11.
Synergy between interleukin-2 and prothymosin α for the increased generation of cytotoxic T lymphocytes against autologous human carcinomas 总被引:3,自引:0,他引:3
Voutsas IF Baxevanis CN Gritzapis AD Missitzis I Stathopoulos GP Archodakis G Banis C Voelter W Papamichail M 《Cancer immunology, immunotherapy : CII》2000,49(8):449-458
Peripheral blood mononuclear cells (PBMC) from cancer patients were cultured in vitro with irradiated autologous tumor cells
isolated from malignant effusions (mixed lymphocyte tumor cultures, MLTC) and low-dose (50 IU/ml) recombinant interleukin-2
(IL-2). The combination of IL-2 and prothymosin α (ProTα) resulted in a greater PBMC-induced response to the autologous tumor
than that brought about by IL-2 alone. In particular, ProTα specifically enhanced the CD4+ T-cell-mediated proliferation against the autologous tumor. CD4+ T cells seemed to recognize tumor antigens presented by HLA-DR molecules expressed on the autologous monocytes, since preincubation
of the latter with an anti-HLA-DR monoclonal antibody (mAb) abrogated the response. In addition, MLTC set up with IL-2 and
ProTα also generated more MHC-class-I-restricted cytotoxic T lymphocytes (CTL) against the autologous tumor than did MLTC
set up with IL-2 alone. The MLTC-induced CTL contained high levels of cytoplasmic perforin and their development was strictly
dependent on the presence of both autologous CD4+ T cells and monocytes. In the absence of either population there was a strong impairment of both proliferative and cytotoxic
responses which was not restored by the presence of ProTα. In contrast, when both cell populations were present, ProTα exerted
optimal enhancement of CD4+ T cell proliferation, which was associated with potentiated CTL responses. Our data emphasize the role of ProTα for the enhancement
of IL-2-induced CTL responses against autologous tumor cells. Such responses require collaborative interactions between CD4+, CD8+ T cells and monocytes as antigen-presenting cells. Our data are relevant for adoptive immunotherapeutic settings utilizing
IL-2 and ProTα-induced autologous-tumor-specific CTL.
Received: 2 March 2000 / Accepted: 1 June 2000 相似文献
12.
The extremely thermophilic archaeon Thermococcus hydrothermalis, isolated from a deep-sea hydrothermal vent in the East Pacific Rise at 21°N, produced an extracellular pullulanase. This
enzyme was purified 97-fold to homogeneity from cell-free culture supernatant. The purified pullulanase was composed of a
single polypeptide chain having an estimated molecular mass of 110 kDa (gel filtration) or 128 kDa (sodium dodecyl sulfate/polyacryl
amide gel electrophoresis). The enzyme showed optimum activity at pH 5.5 and 95 °C. The thermostability and the thermoactivity
were considerably increased in the presence of Ca2+. The enzyme was activated by 2-mercaptoethanol and dithiothreitol, whereas N-bromosuccinimide and α-cyclodextrin were inhibitors. This enzyme was able to hydrolyze, in addition to the α-1,6-glucosidic
linkages in pullulan, α-1,4-glucosidic linkages in amylose and soluble starch, and can therefore be classified as a type II
pullulanase or an amylopullulanase. The purified enzyme displayed Michaelis constant (K
m) values of 0.95 mg/ml for pullulan and 3.55 mg/ml for soluble starch without calcium and, in the presence of Ca2+, 0.25 mg/ml for pullulan and 1.45 mg/ml for soluble starch.
Received: 19 November 1997 / Received revision: 9 March 1998 / Accepted: 14 March 1998 相似文献
13.
Patterns of seed dispersal and the effects of mulching upon Celtis sinensis Pers. seedling establishment were investigated following the removal of this tree weed from a riparian forest community.
At the commencement of the study there was virtually no representation of C. sinensis in the soil seed bank. However, subsequent rates of seed immigration were high since mature individuals of C. sinensis remained on the boundary of the site. Fruit bats (Pteropus spp.) were the principal dispersal vectors. Seed rain density of C. sinensis was best fitted by an inverse power distribution, with seed densities in excess of 20 m−2 detected at 70 m from the seed source. Extrapolation from this relationship suggested that a site would have to be more than
350 m from a seed source to reduce the C. sinensis seed rain to less than 1 m−2. More than 98% of the seed rain occurred below the canopies of the native tree species that remained following the removal
of C. sinensis. For these trees, subarboreal C. sinensis seed distributions were not homogeneous, with peak seed densities occurring at different distances from tree trunks in each
of the two years that seed distributions were assessed. Mulching with compacted sugar cane trash, corresponding to litter
loadings of 6–12 kg m−2, was imposed early in the study, some weeks before the C. sinensis seed rain commenced. These treatments had no measurable effect upon C. sinensis germination, but substantially reduced seedling survival and had variable effects upon the early growth of seedlings. The
potential roles of seed limitation vs establishment limitation are discussed in relation to the management of animal-dispersed
invasive species. It is argued that an understanding of the likely levels and patterns of invasion is essential for the formulation
of management strategies that can effectively reduce the invasion and impacts of these plants.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
14.
M. Baum M. Müller-Steinhardt H. Liesen H. Kirchner 《European journal of applied physiology and occupational physiology》1997,76(2):165-169
The aim of this study was to investigate whether moderate or exhaustive endurance exercise influences cytokine levels in
whole-blood culture supernatants after stimulation. Therefore, eight healthy subjects were first exposed to moderate exercise
on a cycle ergometer for 30 min at 70% of their 4-mmol/l lactic acid (anaerobic) threshold, and 1 week later to exhaustion
(for 90 min) at their anaerobic threshold. Blood samples were taken before, 30 min after and 24 h after each exercise bout.
The following lymphocyte subpopulations were determined: CD14-positive(+)/CD45+, CD4+, CD8+, and CD16+. Cytokine levels in
the supernatants were measured by enzyme-linked immunosorbent assay (ELISA). Production of interleukin (IL)-1β, IL-6 and tumour
necrosis factor (TNF)-α were induced with lipopolysaccharides (LPS), and that of IL-2 and interferon (IFN)-γ with staphylococcal
enterotoxin B (SEB) and phytohaemagglutinin (PHA). Cortisol levels were also determined by ELISA. The lymphocyte subset distribution
was observed to be unchanged after moderate exercise. Thirty minutes after exhaustive exercise, the CD16+ count was found
to be significantly lower, whereas 24 h later the CD4+ count was significantly higher than pre-exercise counts. Moderate exercise
influenced the IFN-γ production (PHA-stimulated), which increased significantly from 974 (391) pg/ml before exercise to 1450 (498) pg/ml
24 h later. Thirty minutes after exhaustive exercise the IFN-γ level in the supernatants (SEB-stimulated) was significantly
decreased (from 14470 (11840) pg/ml before exercise to 6000 (4950) pg/ml after exercise). The IL-1β and TNF-α production per
monocyte was also significantly reduced.
Accepted: 19 February 1997 相似文献
15.
Choi IY Moon PD Koo HN Myung NY Kim SJ Lee JH Han SH Moon G Seo SY Sung HJ Park RK Jeong HJ Um JY Kim HM Hong SH 《In vitro cellular & developmental biology. Animal》2007,43(7):215-221
To explore effects of Forsythia koreana methanol extract (FKME) on mast cell-mediated allergic and inflammatory properties, the effect of FKME was evaluated on compound
48/80-induced systemic anaphylaxis, ear swelling, and anti-dinitrophenyl (DNP) immunoglobulin E (IgE)-induced passive cutaneous
anaphylaxis (PCA). In addition, the effect of FKME was investigated on the histamine release from rat peritoneal mast cells
(RPMCs) stimulated by compound 48/80, which promotes histamine release. The human mast cell line HMC-1 was stimulated by phorbol
12-myristate 13-acetate plus calcium ionophore A23187. Activated HMC-1 can produce several proinflammatory and chemotactic
cytokines such as tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, and IL-8. Cytokine levels in the culture supernatant
were measured by an enzyme-linked immunosorbent assay. Cytotoxicity by FKME was determined by a 3-(4,5-dimethylthiazol-2-yl)-diphenyl-tetrazolium
bromide (MTT) assay. FKME inhibited compound 48/80-induced systemic anaphylactic shock and ear swelling in mice. When 1 g/kg
FKME was pretreated or posttreated with mice, compound 48/80-induced mice morality was 50 and 66.7%, respectively. One gram
per kilogram of FKME pretreatment inhibited ear-swelling responses derived from compound 48/80 by 29.75%. A PCA reaction was
inhibited by 17.9%. In an in vitro model, FKME (1 mg/ml) inhibited histamine release from the RPMCs by 13.8% and TNF-α, IL-6,
and IL-8 production from HMC-1 cells by 71.16% (P < 0.001), 86.72% (P < 0.001), and 44.6%, respectively. However, FKME had no cytotoxic effects on cell viability. In conclusion, FKME inhibited
not only systemic anaphylaxis and ear swelling induced by compound 48/80 but also inhibited a PCA reaction induced by anti-DNP
IgE in vivo. Treatment with FKME showed significant inhibitory effects on histamine, TNF-α, IL-6, and IL-8 release from mast
cells. 相似文献
16.
Aqueous extract of Podophyllum hexandrum (RP-1), which has been reported to render more than 82% survival against whole body lethal (10 Gy) gamma-irradiation in mice,
was further investigated for its immunomodulatory potential. In this study, no significant change could be scored in peritoneal
macrophages survival up to 8th day after whole body irradiation. RP-1 treatment (200 mg/kg body weight, i.p.) alone or 2 h
before whole body irradiation enhanced macrophage survival significantly (p < 0.05) as compared to irradiated control mice. In irradiated animals, there was significant (p < 0.01) reduction in splenocyte survival and proliferation as revealed by 3H-TdR method. RP-1 treatment (200 mg/kg) alone
or 2 h before irradiation countered the decrease in survival of splenocytes and proliferation significantly (p < 0.05) as compared to irradiated control group. Whole body irradiation also significantly (p < 0.05) reduced the population of CD4+ and CD8+ T cells and bone marrow GM-CFU at 24 h and 72 h post-irradiation intervals,
respectively, as compared to unirradiated control. RP-1 treatment 2 h before whole body irradiation countered the decrease
in CD4+ and CD8+ T cells populations and CGM-CFU. Nitric oxide free radicals generation was enhanced significantly (p < 0.05) in the supernatant of peritoneal macrophage cultures exposed to 2 Gy gamma radiation ex vivo in comparison to unirradiated control, which was reduced by pre-irradiation (−2 h) administration of RP-1. Whole body irradiation
(10 Gy) also reduced the serum titres of IL-3, IL-1 and various IgG isotypes observed at different post-irradiation time interval.
RP-1 treatment alone or before whole body irradiation countered radiation induced decrease in the titre of IL-1, IL-3 and
IgG’s in the serum of mice. These findings indicate immunostimulatory potential of RP-1. 相似文献
17.
Tsuyoshi Hosoya Kiyoshi Hamano Michihiro Sugano Yoko Ogura Emiko Hatano Takakazu Hamada 《Mycoscience》1999,40(6):525-529
Dicephalosterol, a new testosterone 5α-reductase inhibitor, was found from isolates ofDicephalospora rufocornea, a sclerotiniaceous discomycete widely distributed, but not previously cultured. Under SEM observation, the polar appendage
of the ascospores inD. rufocornea was found to be more solid than was hitherto reported. Dicephalosterol was produced by submerged fermentation for 7 d. This
new analogue of testosterone showed an IC50 of 5.7 μg/ml for rat prostatic 5α-reductase, but no antimicrobial activity against bacteria or fungi. 相似文献
18.
Daneshmandi S Pourfathollah AA Pourpak Z Heidarnazhad H Kalvanagh PA 《Molecular biology reports》2012,39(2):1845-1853
Asthma is a multifactor inflammatory disorder, and its management requires understanding of its various pathogenesis and control
mechanisms. Cytokines and other inflammatory mediators are important factors in asthma pathophysiology. In this study, we
evaluated the role of cytokine polymorphisms in the asthma susceptibility, progress, control, and lung functions. IL-4-C590T
polymorphism by PCR-RFLP method, IFN-γ T+874A, TNF-α-A308G, IL-6 G−174C and TGF-β T+869C variants by ARMS-PCR method and IgE
serum level by ELISA technique were determined in 81 asthmatic patients and 124 normal subjects. Asthma diagnosis, treatment
and control levels were considered using standard schemes and criteria. TNF-α−308GA genotype was more frequent in asthmatics
(P = 0.025, OR 3.352), and polymorphisms between different asthma control levels (P > 0.05) were not different. IFN-γ+874AT genotype had a positive correlation with the familial history of asthma (P = 0.034, OR 2.688). IL-6−174C allele (P = 0.045), TNF-α−308GG genotype (P = 0.002) and TNF-α−308G allele (P = 0.004) showed reduced values, and TNF-α−308GA genotype (P = 0.002) increased FEF25-75 value in asthmatics. IFN-γ+874AA genotype caused a decrease in FVC factor (P = 0.045). This study showed that TNF-α−308GA is a risk factor for asthma, but cytokine gene variants do not affect asthma
control and IgE serum levels. Variants producing lower levels of IL-6, TNF-α and IFN-γ are associated with reduced pulmonary
capacities. To achieve an appropriate schema for asthma management, further studies with consideration of different aspects
in a larger group of patients would be more elucidative. 相似文献
19.
Wuxia Zhang Yihua Hu Jinzhong Zhao Yongpo Zhang Dongdong Guo Chunyan Gao Jinyou Duan Peng Li 《Glycoconjugate journal》2020,37(3):361-371
Radix Paeoniae Alba is widely used in Chinese traditional medicine to treat various diseases such as gastrointestinal disorders, immunomodulatory, cancer, and other diseases. In this paper, a novel acidic polysaccharide RPAPS purified from Radix Paeoniae Alba was evaluated for its structural features and potential of immunomodulatory and antioxidant activities. RPAPS (molecular weight: 1.0× 105 Da) was mainly composed of α-(1 → 4)-Glcp, α-Arap, α-Galp, α-Rhap, β-D-Glcp, α-(1 → 6)-linked Glcp and GalA. Immunological tests indicated that RPAPS could improve RAW264.7 phagocytic activity and LPS-induced splenocyte proliferation. For antioxidant activities, RPAPS showed reducing power and DPPH scavenging activity in dose dependent. Moreover, RPAPS could significantly protect the PC12 cells from H2O2 damage. These data implied polysaccharides RPAPS had the potential to be novel natural antioxidative and immunopotentiating agents for using in functional foods or medicine. 相似文献
20.
Rapid propagation technology was established and optimized in vitro for Chrysanthemum cinerariifolium (Trev.) Vis., an important botanical insecticide plant with a huge international market. A large number of buds could be
induced directly from epicotyl and hypocotyl explants on Murashige T; Skoog F. J. Plant. Physiol. 15: 473–479; (1962) medium
[Murashige and Skoog (MS) medium] supplemented with 0.3 mg l−1 benzyladenine (BA) and 0.3 mg l−1 α-naphthaleneacetic acid (NAA). Root induction and development could be observed within 15 d after inoculation on 1/2 MS
medium supplemented with 0.2 mg l−1 indole-3-acetic acid (IAA) and 0.1 mg l−1 rooting powder (ABT). Furthermore, a polyploid breeding study in vitro was reported to obtain superior breeding lines with high yield and good quality. Autotetraploid lines of C. cinerariifolium were obtained by colchicine treatments and identified by root-tip chromosome determination and stoma observation. The chromosome
number of the autotetraploid plantlet was 2N = 4x = 36. Obtained autotetraploid lines will be of important genetic and breeding value and be used for further selection and
plant breeding. 相似文献