Discrimination of heterozygote currency of ataxia-telangiectasia by indirect immunofluorescent analysis

The qualitative differences in P53 protein stabilization after ionizing irradiation in different doses were found in cells of members of ataxia-telangiectasia (AT) family--proband AT6SP, her sister AT(S)6SP and father AT(F)6SP. The method of indirect immunofluorescence with confocal microscopy was used.     

Presence and indirect immunofluorescent localization of calmodulin in Paramecium tetraurelia

In this paper we demonstrate the presence and localization of calmodulin, a calcium-dependent regulatory protein, in the ciliated protozoan Paramecium tetraurelia. Calmodulin is demonstrated by several criteria: (a) the ability of whole cell Paramecium extracts to stimulate mammalian phosphodiesterase activity, (b) the presence of an acidic, thermostable, 17,000-dalton polypeptide whose mobility shifts in SDS polyacrylamide gel electrophoresis in the presence of Ca2+, and (c) the affinity of antibodies against mammalian calmodulin for a Paramecium component as demonstrated by both indirect immunofluorescent localization and radioimmunoassay. Indirect immunofluorescence studies reveal that Paramecium calmodulin is distributed in three distinct regions of the cell, i.e., (a) large, spherical cytoplasmic organelles representing perhaps the food vacuoles or other vacuolar inclusions of the cell, (b) along the entire length of oral and somatic cilia, and (c) along a linear punctate pattern corresponding to the kinetics (basal bodies) of the cell.     

Discrimination of heterozygous carriage of ataxia-telangiectasia by the method of indirect immunofluorescent analysis

Qualitative differences of the stabilization of protein P53 were studied by methods of indirect immunofluorescence with the use of confocal microscopy in cells obtained from members of the family of a patient with ataxia-telangiectasia (AT)—the proband AT6SP and her blood relatives: the sister AT(S)6SP and the father AT(F)6SP—at different time periods after irradiation with various doses of ionizing radiation.     

The indirect hemagglutination reaction in the diagnosis of trichinosis

An indirect hemagglutination test (IHAT) for trichinosis using a Melcher's antigen was evaluated and compared with a precipitin test (PT) and a bentonite flocculation test (BFT). One hundred and forty eight serum samples from patients from the whole country confirmed or suspected to have trichinosis by clinical or epidemiological evidences were studied: 117 (79.1%) samples resulted positive by IHAT, 138 (93.2%) by PT and 65 (43.9%) by BFT. Sixty three serum samples from patients with strong clinical suspect of trichinosis presented the PT and the BFT positive and were compared with the IHAT for sensitivity study. IHAT was positive in 60 (95.2%) serum samples. In order to determine the specificity of IHAT 25 serum samples from healthy volunteers and 124 serum samples from individuals with other parasitoses, such as cysticercosis (48), hydatidosis (45) and fascioliasis (31) were studied. The specificity, using a titre > or = 1:16 as a possible diagnostic value was 96%. The use of IHAT with RP and BFT in the diagnosis of human trichinosis is discussed.     

Investigation of anti-Toxoplasma gondii antibodies in cats of the Ankara region of Turkey Using the Sabin-Feldman dye test and an indirect fluorescent antibody test

Blood samples from 99 cats from the Ankara province of Turkey were examined for the presence of anti-Toxoplasma gondii antibody with the use of both the Sabin-Feldman dye test (DT) and an indirect fluorescent antibody test (IFAT). Forty of the 99 sera (40.3%) were positive for antibodies against T. gondii with the DT, whereas the IFAT assay detected antibodies in 34 (34.3%). The study also evaluated 3 factors for their potential association with the presence of T. gondii antibody: age (<1 yr, 1-2 yr, and >2 yr), gender (female vs. male), and outdoor access (stray, owned with outdoor access, or indoor only). The DT detected antibodies in 3 cats under 1 yr of age, 22 cats between 1 and 2 yr, and 15 cats older than 2 yr, whereas the IFAT found 1, 18, and 15 cats positive for antibodies, respectively, in each of these categories. Of 61 female cats, 27 (44.2%) were positive by the DT; and of 38 male cats, 13 (34%) were positive by the DT. For the IFAT, 24 female cats (39.3%) and 10 male cats (26.3%) were positive. The percent seropositivity in indoor cats was 30.8% by the DT and 23.1% by the IFAT. In stray cats, the percent seropositivity was 52.8% by the DT and 41.7% by the IFAT. Antibody presence was significantly associated with age, but not with outdoor access.     

Use of the immunofluorescent microagglutination reaction for the intrageneric differentiation of the immune response in chlamydiosis

The possibility of using, on principle, the immunofluorescent microagglutination test with Chlamydia fluorescent corpuscular antigen for the strain-specific differentiation of immune response induced by the causative agents of ornithosis (strain B), enzootic abortus ovis (strain EAE) and lymphogranuloma venereum (strain LV) has been experimentally demonstrated. The calculation of indices, characterizing the specificity of differences between the systems under comparison, by the method of S. Frasser and D. Berman (1965) has confirmed the significance of such differentiation.     

Phosphoenolpyruvate carboxylase in guard cells of several species as determined by an indirect,immunofluorescent technique

Summary Phosphoenolpyruvate carboxylase (E. C. 4.1.1.31) was localized in the guard cells by an indirect, immunofluorescent technique using antisera raised against the enzyme from bothPennisetum glaucum R. Br. (pearl millet) andEscherichia coli. Of the 17 species of plants examined, only monocot species showed fluorescence with millet phosphoenolpyruvate carboxylase antiserum and onlyNephrolepis exaltata (L.) Schott,Zygocactus truncatus Haworth, andEcheveria gibbiflora D. C. displayed fluorescence withE. coli phosphoenolpyruvate carboxylase antiserum.