微生态调节剂黄瓜香治疗细菌性阴道炎疗效观察

目的 观察微生态调节剂黄瓜香治疗细菌性阴道炎的疗效.方法 用黄瓜香提取液治疗细菌性阴道炎,治疗前后分别进行测阴道分泌物pH、做线索细胞和胺试验检查,进行总有效率评定.结果 经2种药物治疗后所有患者临床症状均明显减轻,治疗前后自身比较差异有显著性(P<0.01); 83%以上的患者呈现线索细胞转(-)、胺试验转(-) ,超过一半的患者阴道分泌物pH低于4.5;临床治疗总有效率分别为76.66%和79.4%%.2种药物组间比较治疗效果差异无显著性(P>0.05).结论 阴道补充黄瓜香与使用甲硝唑治疗产生了相似的效果,有可能成为治疗细菌性阴道炎的新药.     

清消法对痤疮患者面部皮肤微生态的调节作用

通过观察皮肤酸碱度、皮脂分泌率、皮损部细菌密度、多样性和局部IL-1、IL-6水平,探讨清消法对寻常型痤疮患者面部微生态的影响。

选择我院收治的寻常型痤疮患者142例,按照随机数字表分成治疗组和对照组,各71例。对照组患者口服异维A酸联合外涂阿达帕林凝胶、夫西地酸乳膏;治疗组患者采用清消法联合外涂阿达帕林凝胶、夫西地酸乳膏进行治疗。观察两组患者的临床疗效、痤疮综合分级系统（GAGS）评分、面部皮肤酸碱度、皮脂分泌率、皮损部细菌密度和多样性及皮损局部IL-1、IL-6水平。

治疗组患者的总有效率（92.96%）高于对照组（81.69%）。与对照组比较,治疗组患者GAGS评分、皮肤酸碱度、皮脂分泌率、皮损部细菌密度及IL-1、IL-6水平均显著降低（均P＜0.05）。

清消法可通过调节面部油脂分泌和酸碱度,改善患者皮损部菌群失衡,降低炎症因子,从而有效治疗痤疮。

     

黄瓜香醇提取液治疗细菌性阴道炎疗效观察

目的观察黄瓜香醇提取液治疗细菌性阴道炎的疗效。方法用黄瓜香醇提取液治疗细菌性阴道炎,治疗前后分别测阴道分泌物p H、做线索细胞和胺试验检查,进行总有效率评定。结果黄瓜香醇提取液治疗后所有患者临床症状均明显减轻,治疗前后自身比较差异有统计学意义(P0.01);83%以上的患者呈现线索细胞转(-)、胺试验转(-),超过一半的患者阴道分泌物p H低于4.5;临床治疗总有效率为76.66%。结论黄瓜香醇提取液能够治疗细菌性阴道炎,有可能成为治疗细菌性阴道炎的新药。     

生态蛇胆霜治疗寻常痤疮及皮肤菌群变化的研究

本文报告了应用微生态制剂－生态蛇胆霜治疗寻常痤疮３０例，取得总有效率９０．０％的较好疗效，且无任何不良反应。并对治疗前后皮肤菌群变化与健康人对比及疗前疗后里面形螨早数量变化进行了研究。     

中药面膜联合红蓝光治疗寻常痤疮疗效观察

目的：观察中药面膜联合红蓝光治疗仪治疗寻常痤疮临床疗效及安全性。方法：66例寻常痤疮患者按就诊先后顺序随机分为3组：对照组、面膜组、联合组,治疗4周后进行疗效评估,治疗结束后4周随访复发情况。结果：联合组及面膜组疗效高于对照组（P〈0.05）。结论：中药面膜联合红蓝光治疗仪治疗寻常痤疮疗效好,不良反应少,优于单用疗法,痤疮后期维持治疗同样值得重视。     

生态蛇胆霜治疗寻常痤疮及对皮肤菌群变化的研究

本文报告了应用微生态制剂生态蛇胆霜治疗寻常座疮３０例,取得了总有效率９０．０％的较好疗效,且无任何不良反应。并对治疗前后皮肤菌群变化与健康人对比及疗前疗后蠕形螨虫数量变化进行了研究。结果显示痤疮病人面部常住菌及过路菌数量明显高于健康人（Ｐ＜０．０１）疗后寄生菌与疗前比较差异显著（Ｐ＜０．０１）。而与健康人相比无显著性差异（Ｐ＜０．０５）,治疗前后面部蠕形螨检测结果证实本品有抑制蠕形螨虫生长作用。     

黄瓜香等对调整腹水瘤小鼠肠道菌群并延长荷瘤生存时间的影响

目的研究黄瓜香等在调节肠道菌群和改善荷瘤小鼠生存质量,提高生存率方面的作用。方法用肝癌腹水瘤H22细胞株注射小鼠造荷瘤小鼠模型,然后口服黄瓜香提取液治疗。观察治疗前后菌群变化、腹水量、荷瘤生存时间等。结果中药组与阴性对照组比较,肠道菌群趋于平衡、腹水出现时间延迟、腹水量降低、荷瘤生存时间延长。结论黄瓜香等能调节小鼠肠道菌群,改善荷瘤小鼠生存质量、提高生存率。     

黄瓜香等中药调整腹水瘤小鼠肠道菌群减轻化疗副作用的研究

目的通过黄瓜香等中药调整肝癌腹水瘤小鼠肠道菌群以减轻化疗的副作用达到预防和治疗肿瘤的目的。方法以小鼠腹腔内注射肝癌H22腹水瘤细胞造成肝癌腹水瘤模型,分别于造模型前1周用黄瓜香等和造模型后5 d用顺铂加上黄瓜香等进行治疗,并于注射后做HE染色检测肿瘤种植情况、血中白细胞、血小板的数量及巨噬细胞吞噬能力等。结果中药预防组与对照组相比肿瘤种植明显降低,中药加化疗组与阴性对照组比血中红细胞、白细胞和血小板均有回升(P0.01),巨噬细胞吞噬率增加明显。结论黄瓜香等能调节机体免疫力,防治腹水瘤和减轻化疗的副作用。     

耳穴贴压配合面部护理治疗寻常痤疮48例体会

方法:用耳穴贴压配合面部护理治疗寻常痤疮。结果:经2-4疗程治疗,总有效率为90%,获得较好疗效。结论:耳穴贴压具有调节脏腑功能,调节内分泌,抑制过多的雄激素的作用,配合面部皮肤护理,从根本上去除痤疮的发病机制,且经济、便捷、无副作用。     

STA-21治疗寻常型银屑病的疗效观察

目的:观察STA-21治疗寻常型银屑病的临床疗效与安全性.方法:分别在13例寻常型银屑病患者双下肢选取临床表现、大小和部位相一致的皮损,分另外用0.2％STA-21软膏和软膏基质治疗2周,记录治疗前后的PASI分值.结果:治疗组有效率为84.61％,对照组为30.77％,差异有统计学意义(x=4.887,P=0.027).两组均未无明显不良反应.结论:STA-21治疗寻常型银屑病有较好的疗效与安全性.     

寻常痤疮中的微生态机制研究

最近的一项研究发现健康人群和寻常痤疮患者面部及肠道的菌群存在显著差异,寻常痤疮患者面部及肠道菌群出现紊乱,并且发现寻常痤疮并不与痤疮丙酸杆菌的过度增殖有关,而是取决于菌株的类型及其之间的平衡。此外,肠道菌群的紊乱也会间接影响寻常痤疮的发生和发展。因此,越来越多的研究致力于改善寻常痤疮患者面部和肠道的菌群平衡,进而达到治疗寻常痤疮的目的。本文主要综述了寻常痤疮患者面部和肠道的菌群失衡与寻常痤疮发病的关系,以及改善寻常痤疮患者面部和肠道菌群失衡的益生菌疗法。

     

Peptides with antimicrobial and anti-inflammatory activities that have therapeutic potential for treatment of acne vulgaris

The pathogenesis of acne vulgaris is multifactorial involving infection of the pilosebaceous unit with Propionibacterium acnes and a cytokine-mediated inflammatory response. Five frog skin-derived antimicrobial peptides ([D4k]ascaphin-8, [G4K]XT-7, [T5k]temporin-DRa, brevinin-2GU, and B2RP-ERa), chosen for their low hemolytic activity against human erythrocytes, were assessed for their effects on the growth of clinical isolates of P. acnes and on the release of pro-inflammatory and anti-inflammatory cytokines from peripheral blood mononuclear (PBM) cells. All peptides inhibited the growth of P. acnes with the highest potency exhibited by [D4k]ascaphin-8 (minimum inhibitory concentration, MIC=3-12.5 μM). Release of TNF-α from concanavalin A (ConA)-stimulated PBM cells was significantly reduced by [D4k]ascaphin-8, [G4K]XT-7, brevinin-2GU, and B2RP-ERa (1 and 20 μg/ml) and by [T5k]temporin-DRa (20 μg/ml). Release of IFN-γ from unstimulated PBM cells was significantly reduced by [D4k]ascaphin-8 and brevinin-2GU (1 and 20 μg/ml). No peptide showed significant effects on Il-17 release. Release of the anti-inflammatory cytokines TGF-β, IL-4, and IL-10 from both unstimulated and ConA-treated PBM cells was significantly increased by [T5k]temporin-DRa and B2RP-ERa (1 and 20μg/ml). The potent activities of [D4k]ascaphin-8 and [T5k]temporin-DRa in inhibiting the growth of P. acnes and the release of pro-inflammatory cytokines, and in stimulating the release of anti-inflammatory cytokines suggest a possible therapeutic role in the treatment of acne vulgaris.     

Association of TNF-α polymorphisms (−857, −863 and −1031), TNF-α serum level and lipid profile with acne vulgaris

BackgroundAcne is an inflammatory condition principally affected by genetic and dietary factors. Investigation into functional polymorphisms of TNF-α gene and their association with acne vulgaris will be helpful in exploring genetic influence on skin immune mediated inflammatory events. In the present study, we analyzed association of TNF-α gene polymorphisms, its expression levels and lipid profiles in a large cohort of acne patients and controls.MethodsWe used PCR-RFLP to study association of TNF-α polymorphisms at −857C/T, −863C/A and −1031 T/C sites with acne vulgaris. Lipid profiles were measured using enzymatic end-point method. The serum levels of TNF-α and apolipoprotein a were measured using ELISA. NIH, LDlink was used to investigate patterns of linkage disequilibrium across south Asian reference genome (Punjabi from Lahore Pakistan).ResultsWe found that TNF-α −863 polymorphism is strongly associated with acne in overall population as well as in gender and severity based groups of acne patients. Polymorphisms at −863 and −1031 position were in linkage disequilibrium. Importantly, TNF-α serum level was significantly increased in acne patients with severe disease symptoms. Furthermore, levels of total cholesterol (TC) and triglycerides (TG) were significantly increased, whereas high density lipoprotein cholesterol (HDL-C) level was significantly decreased in acne patients. The levels of apolipoprotein a varied widely in studied populations and no significant difference was found in the analyzed groups.ConclusionIn conclusion, we found that TNF-α expression increases in acne patients affected by TNF-α polymorphisms, and that the lipid profile is specifically disrupted in acne patients.     

磁性纳米微粒分离中药蛋白质的实验研究

目的:探讨并研究磁性纳米微粒对不同种生物样品中蛋白质的分离方法及效果。方法:磁性纳米微粒与AEAPS反应后,表面修饰并连接-NH2制成蛋白质分离器,通过氨基与羧基的结合及外磁力作用分离蛋白质。结果:MNP-NH2对BSA的结合呈现直线相关(r=0.9942,p<0.001)。对几种来源不同的生物样品中可溶性蛋白的分离结果显示,各实验组数值与对照组比较,均具有显著性差异(P<0.05～0.001)。结论:MNP-NH2对于分离生物样品中蛋白质具有较好的分离效果对蛋白质活性影响较小。     

中草药复方对草鱼鱼种肠道菌群与血液有形成分的影响

目的探讨中草药复方对鱼种肠道菌群和血液有形成分的影响。方法以草鱼鱼种为动物模型,分别采用平板倾注法和ATB半自动化细菌鉴定法,测定投喂中草药复方药饵前、后鱼种肠道菌群数量及其优势菌群种类。同时使用常规计数法和改良沙利法,测定鱼种血液中红细胞、白细胞和血红蛋白的含量。结果对照组鱼种肠道内的细菌总数为6．6×10^8CFU／g,其优势菌群为气单胞菌、假单胞菌和肠杆菌;投喂2．5％、5．0％和7．5％中草药复方药饵10d后,其肠道内细菌总数均有所减少,分别为3．0×10^7CFU／g、5．0×10^6CFU／g和1．5×10^7CFU／g,其优势菌群为吲哚黄杆菌、产碱假单胞菌和野油菜黄单胞菌。实验组与对照组鱼种的红细胞数量差异无显著性,但白细胞与血红蛋白的含量差异有非常显著性（P〈0．01）。结论以黄连、大黄、金银花、连翘、黄芪和甘草组成的中草药复方对鱼种肠道菌群和血液有形成分均有一定影响,表现出该复方具有较好的免疫抗病作用。     

Antimicrobial susceptibilities of Propionibacterium acnes isolated from patients with acne vulgaris

Antibiotic susceptibilities of Propionibacterium acnes in Japan were determined. Erythromycin‐resistance was found in 10.4% (5/48) of the strains, and four of these were cross‐resistance to clindamycin. Although the erythromycin ribosome methylase gene erm(X) was looked for, no strain carrying erm(X) was found. Sequencing analysis revealed that all of the erythromycin‐resistant strains had a mutation in the peptidyl transferase region of the 23S rRNA gene: G2057A, A2058G, or A2059G. Consequently, our results show that P. acnes resistance to macrolides is caused by a mutation in the 23S rRNA gene, and has been increasing in Japan.     

Anti-inflammatory activity of cationic peptides: application to the treatment of acne vulgaris

Cationic antimicrobial peptides exhibit potent antimicrobial activity against clinically relevant microorganisms including Propionibacterium acnes. Recent studies showed that, in addition to the antimicrobial activity, these peptides can exhibit an anti-inflammatory effect. These properties make cationic peptides attractive drug candidates for the treatment of acne vulgaris, a disease with both bacterial and inflammatory components. This review focuses on the anti-inflammatory activity of cationic antimicrobial peptides and its application for the treatment of acne vulgaris. The anti-inflammatory activity of cationic peptides in acne vulgaris can be explained by their ability to both bind proinflammatory bacterial factors (e.g. lipoteichoic acid), sequestering them from the site of inflammation, and to inhibit the secretion of proinflammatory cytokines (e.g. tumor necrosis factor-alpha, IL-1) by host cells. These anti-inflammatory effects combined with potent antimicrobial activity may translate into a novel therapeutic option for acne vulgaris.     

丽珠肠乐治疗溃疡性结肠炎的临床疗效及病理变化的研究

目的:探讨溃疡性结肠炎的微生态学改变及双歧杆菌对溃疡性结肠炎的预防和治疗作用.方法:随机选取溃疡性结肠炎的患者60人,分成二组.治疗组:口服丽珠肠乐胶囊;对照组:口服氟哌酸胶囊,柳氮磺胺吡啶栓直肠给药.疗程一个月.结果:溃疡性结肠炎的肠道内细菌数量双歧杆菌、乳杆菌显著下降(P＜0.01),肠球菌显著增加(P＜0.01).经丽珠肠乐治疗后,肠道内主要细菌的数量恢复正常,与对照组相比差异有显著性(P＜0.01);肠道黏膜病理及组织学改变基本恢复正常;临床症状改善上治疗组疗效高于对照组(P＜0.01).结论:双歧杆菌活菌制剂治疗溃疡性结肠炎效果显著,值得临床推广使用.