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1.
DNA barcoding was used in the identification of 89 commercially important freshwater and marine fish species found in Turkish ichthyofauna. A total of 1765 DNA barcodes using a 654‐bp‐long fragment of the mitochondrial cytochrome c oxidase subunit I gene were generated for 89 commercially important freshwater and marine fish species found in Turkish ichthyofauna. These species belong to 70 genera, 40 families and 19 orders from class Actinopterygii, and all were associated with a distinct DNA barcode. Nine and 12 of the COI barcode clusters represent the first species records submitted to the BOLD and GenBank databases, respectively. All COI barcodes (except sequences of first species records) were matched with reference sequences of expected species, according to morphological identification. Average nucleotide frequencies of the data set were calculated as T = 29.7%, C = 28.2%, A = 23.6% and G = 18.6%. Average pairwise genetic distance among individuals were estimated as 0.32%, 9.62%, 17,90% and 22.40% for conspecific, congeneric, confamilial and within order, respectively. Kimura 2‐parameter genetic distance values were found to increase with taxonomic level. For most of the species analysed in our data set, there is a barcoding gap, and an overlap in the barcoding gap exists for only two genera. Neighbour‐joining trees were drawn based on DNA barcodes and all the specimens clustered in agreement with their taxonomic classification at species level. Results of this study supported DNA barcoding as an efficient molecular tool for a better monitoring, conservation and management of fisheries. 相似文献
2.
Implications of High Molecular Divergence of Nuclear rRNA and Phylogenetic Structure for the Dinoflagellate Prorocentrum (Dinophyceae,Prorocentrales)
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The small and large nuclear subunit molecular phylogeny of the genus Prorocentrum demonstrated that the species are dichotomized into two clades. These two clades were significantly different (one‐factor ANOVA, p < 0.01) with patterns compatible for both small and large subunit Bayesian phylogenetic trees, and for a larger taxon sampled dinoflagellate phylogeny. Evaluation of the molecular divergence levels showed that intraspecies genetic variations were significantly low (t‐test, p < 0.05), than those for interspecies variations (> 2.9% and > 26.8% dissimilarity in the small and large subunit [D1/D2], respectively). Based on the calculated molecular divergence, the genus comprises two genetically distinct groups that should be considered as two separate genera, thereby setting the pace for major systematic changes for the genus Prorocentrum sensu Dodge. Moreover, the information presented in this study would be useful for improving species identification, detection of novel clades from environmental samples. 相似文献
3.
R. A. López‐Pérez L. E. Calderon‐Aguilera R. C. Zepeta‐Vilchis I. López Pérez Maldonado A. M. López Ortiz 《Zeitschrift fur angewandte Ichthyologie》2013,29(2):437-448
In spite of their ecological and economic importance, reef fishes from the coast of Oaxaca, Mexico are rarely studied, therefore precluding their management and conservation. In order to identify the set of habitat characteristics/environmental conditions that predict major shifts in fish assemblages in space and time, a stationary census (5′, φ = 5 m) was conducted on a semi‐monthly basis from 2006 to 2009 at patch reefs along the coast. Habitat configuration was gathered using 25 m long point‐intersect transects (data every 25 cm), recording all underlying coral species and substrate characteristics (rocks, sand, algal mats, rubble or dead corals). Recorded were 65 452 fishes grouped in 11 orders, 36 families, 65 genera and 89 species. Labridae (nine species), Pomacentridae (eight species) and Serranidae (seven species) were the most frequent families. Abundance is severely skewed among species; four species Thalassoma lucasanum, Chromis atrilobata, Apogon pacificus and Stegastes acapulcoensis comprise nearly 59% of the fish abundance, 11 species contribute 30%, whereas most of the species (75) can be considered as rare since they contribute <1% each to the total. Species richness and family‐level assemblage composition are similar to those recorded elsewhere in the eastern Pacific. Non‐parametric multivariate analysis of variance demonstrated that changes of diversity metrics might be associated with environmental differences on the scale of hundreds of meters to kilometers, as well as coupled with major changes on oceanographic variables throughout time, exerting meaningful changes on reef‐related fish assemblages. 相似文献
4.
Erik E. Sotka Tina Bell Lauren E. Hughes James K. Lowry Alistair G. B. Poore 《Zoologica scripta》2017,46(1):85-95
Ampithoid amphipods dominate invertebrate assemblages associated with shallow‐water macroalgae and seagrasses worldwide and represent the most species‐rich family of herbivorous amphipod known. To generate the first molecular phylogeny of this family, we sequenced 35 species from 10 genera at two mitochondrial genes [the cytochrome c oxidase subunit I (COI) and the large subunit of 16 s (LSU)] and two nuclear loci [sodium–potassium ATPase (NAK) and elongation factor 1‐alpha (EF1)], for a total of 1453 base pairs. All 10 genera are embedded within an apparently monophyletic Ampithoidae (Amphitholina, Ampithoe, Biancolina, Cymadusa, Exampithoe, Paragrubia, Peramphithoe, Pleonexes, Plumithoe, Pseudoamphithoides and Sunamphitoe). Biancolina was previously placed within its own superfamily in another suborder. Within the family, single‐locus trees were generally poor at resolving relationships among genera. Combined‐locus trees were better at resolving deeper nodes, but complete resolution will require greater taxon sampling of ampithoids and closely related outgroup species, and more molecular characters. Despite these difficulties, our data generally support the monophyly of Ampithoidae, novel evolutionary relationships among genera, several currently accepted genera that will require revisions via alpha taxonomy and the presence of cryptic species. 相似文献
5.
Lichun Jiang Liqing Peng Min Tang Zhangqiang You Min Zhang Andrea West Qiping Ruan Wei Chen Juha Meril 《Ecology and evolution》2019,9(15):8813-8828
This is the first study to describe the mitochondrial genome of the Himalayan Griffon, Gyps himalayensis, which is an Old World vulture belonging to the family Accipitridae and occurring along the Himalayas and the adjoining Tibetan Plateau. Its mitogenome is a closed circular molecule 17,381 bp in size containing 13 protein‐coding genes, 22 tRNA coding genes, two rRNA‐coding genes, a control region (CR), and an extra pseudo‐control region (CCR) that are conserved in most Accipitridae mitogenomes. The overall base composition of the G. himalayensis mitogenome is 24.55% A, 29.49% T, 31.59% C, and 14.37% G, which is typical for bird mitochondrial genomes. The alignment of the Accipitridae species control regions showed high levels of genetic variation and abundant AT content. At the 5′ end of the domain I region, a long continuous poly‐C sequence was found. Two tandem repeats were found in the pseudo‐control regions. Phylogenetic analysis with Bayesian inference and maximum likelihood based on 13 protein‐coding genes indicated that the relationships at the family level were (Falconidae + (Cathartidae + (Sagittariidae + (Accipitridae + Pandionidae))). In the Accipitridae clade, G. himalayensis is more closely related to Aegypius monachus than to Spilornis cheela. The complete mitogenome of G. himalayensis provides a potentially useful resource for further exploration of the taxonomic status and phylogenetic history of Gyps species. 相似文献
6.
Morphological innovation,ecological opportunity,and the radiation of a major vascular epiphyte lineage
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Michael A. Sundue Weston L. Testo Tom A. Ranker 《Evolution; international journal of organic evolution》2015,69(9):2482-2495
The emergence of angiosperm‐dominated tropical forests in the Cretaceous led to major shifts in the composition of biodiversity on Earth. Among these was the rise to prominence of epiphytic plant lineages, which today comprise an estimated one‐quarter of tropical vascular plant diversity. Among the most successful epiphytic groups is the Polypodiaceae, which comprises an estimated 1500 species and displays a remarkable breadth of morphological and ecological diversity. Using a time‐calibrated phylogeny for 417 species, we characterized macroevolutionary patterns in the family, identified shifts in diversification rate, and identified traits that are potential drivers of diversification. We find high diversification rates throughout the family, evidence for a radiation in a large clade of Paleotropical species, and support for increased rates of diversification associated with traits including chlorophyllous spores and noncordiform gametophytes. Contrary to previous hypotheses, our results indicate epiphytic species and groups with humus‐collecting leaves diversify at lower rates than the family as a whole. We find that diversification rates in the Polypodiaceae are positively correlated with changes in elevation. Repeated successful exploration of novel habitat types, rather than morphological innovation, appears to be the primary driver of diversification in this group. 相似文献
7.
Animals use and select habitat at multiple hierarchical levels and at different spatial scales within each level. Still, there is little knowledge on the scale effects at different spatial levels of species occupancy patterns. The objective of this study was to examine nonlinear effects and optimal‐scale landscape characteristics that affect occupancy of the Siberian flying squirrel, Pteromys volans, in South‐ and Mid‐Finland. We used presence–absence data (n = 10,032 plots of 9 ha) and novel approach to separate the effects on site‐, landscape‐, and regional‐level occupancy patterns. Our main results were: landscape variables predicted the placement of population patches at least twice as well as they predicted the occupancy of particular sites; the clear optimal value of preferred habitat cover for species landscape‐level abundance is a surprisingly low value (10% within a 4 km buffer); landscape metrics exert different effects on species occupancy and abundance in high versus low population density regions of our study area. We conclude that knowledge of regional variation in landscape utilization will be essential for successful conservation of the species. The results also support the view that large‐scale landscape variables have high predictive power in explaining species abundance. Our study demonstrates the complex response of species occurrence at different levels of population configuration on landscape structure. The study also highlights the need for data in large spatial scale to increase the precision of biodiversity mapping and prediction of future trends. 相似文献
8.
Morphological assessment of the Octopus vulgaris species complex evaluated in light of molecular‐based phylogenetic inferences
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Michael D. Amor Mark D. Norman Alvaro Roura Tatiana S. Leite Ian G. Gleadall Amanda Reid Catalina Perales‐Raya Chung‐Cheng Lu Colin J. Silvey Erica A. G. Vidal Frederick G. Hochberg Xiaodong Zheng Jan M. Strugnell 《Zoologica scripta》2017,46(3):275-288
Cryptic species are common in the ocean, particularly among marine invertebrates such as octopuses. Delineating cryptic species is particularly problematic in octopus taxonomy where the plasticity recorded among taxonomic characters often results in low resolution at the species level. This study investigated the morphological relationships among seven phylogenetic clades (identified using cytochrome c oxidase subunit I) of the broadly distributed Octopus vulgaris species complex and close relatives. Morphological analyses in this study were successful in delimiting O. sinensis, Brazilian O. vulgaris and O. vulgaris sensu stricto, which was congruent with the molecular findings of this study. Analyses based on male morphology were successful in distinguishing 14 of 15 total pairwise comparisons and proved to be a more reliable indicator of species‐level relationships in comparison with female morphology. The majority of characters with the greatest discriminatory power were male sexual traits. Significant morphological differences were also recorded among sampling localities of conspecifics, with phenotype showing correlation with local environmental data. The findings of this study support the hypothesis that multiple O. vulgaris‐like species are currently being incorrectly treated under a single species name, O. vulgaris. Octopuses being exported globally under the name O. vulgaris are of extremely high fisheries market value and profile. Our findings have potentially significant implications for the naming and conservation of commercially harvested members of this species complex throughout their ranges. 相似文献
9.
Sequence variation in nuclear ribosomal small subunit,internal transcribed spacer and large subunit regions of Rhizophagus irregularis and Gigaspora margarita is high and isolate‐dependent
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Odile Thiéry Martti Vasar Teele Jairus John Davison Christophe Roux Paula‐Ann Kivistik Andres Metspalu Lili Milani Ülle Saks Mari Moora Martin Zobel Maarja Öpik 《Molecular ecology》2016,25(12):2816-2832
Arbuscular mycorrhizal (AM) fungi are known to exhibit high intra‐organism genetic variation. However, information about intra‐ vs. interspecific variation among the genes commonly used in diversity surveys is limited. Here, the nuclear small subunit (SSU) rRNA gene, internal transcribed spacer (ITS) region and large subunit (LSU) rRNA gene portions were sequenced from 3 to 5 individual spores from each of two isolates of Rhizophagus irregularis and Gigaspora margarita. A total of 1482 Sanger sequences (0.5 Mb) from 239 clones were obtained, spanning ~4370 bp of the ribosomal operon when concatenated. Intrasporal and intra‐isolate sequence variation was high for all three regions even though variant numbers were not exhausted by sequencing 12–40 clones per isolate. Intra‐isolate nucleotide variation levels followed the expected order of ITS > LSU > SSU, but the values were strongly dependent on isolate identity. Single nucleotide polymorphism (SNP) densities over 4 SNP/kb in the ribosomal operon were detected in all four isolates. Automated operational taxonomic unit picking within the sequence set of known identity overestimated species richness with almost all cut‐off levels, markers and isolates. Average intraspecific sequence similarity values were 99%, 96% and 94% for amplicons in SSU, LSU and ITS, respectively. The suitability of the central part of the SSU as a marker for AM fungal community surveys was further supported by its level of nucleotide variation, which is similar to that of the ITS region; its alignability across the entire phylum; its appropriate length for next‐generation sequencing; and its ease of amplification in single‐step PCR. 相似文献
10.
Chris C. Nice Matthew L. Forister Joshua G. Harrison Zachariah Gompert James A. Fordyce James H. Thorne David P. Waetjen Arthur M. Shapiro 《Global Change Biology》2019,25(6):2127-2136
Certain general facets of biotic response to climate change, such as shifts in phenology and geographic distribution, are well characterized; however, it is not clear whether the observed similarity of responses across taxa will extend to variation in other population‐level processes. We examined population response to climatic variation using long‐term incidence data (collected over 42 years) encompassing 149 butterfly species and considerable habitat diversity (10 sites along an elevational gradient from sea level to over 2,700 m in California). Population responses were characterized by extreme heterogeneity that was not attributable to differences in species composition among sites. These results indicate that habitat heterogeneity might be a buffer against climate change and highlight important questions about mechanisms maintaining interpopulation differences in responses to weather. Despite overall heterogeneity of response, population dynamics were accurately predicted by our model for many species at each site. However, the overall correlation between observed and predicted incidence in a cross validation analysis was moderate (Pearson's r = 0.23, SE 0.01), and 97% of observed data fell within the predicted 95% credible intervals. Prediction was most successful for more abundant species as well as for sites with lower annual turnover. Population‐level heterogeneity in response to climate variation and the limits of our predictive power highlight the challenges for a future of increasing climatic variability. 相似文献
11.
Phylogenetic placement of Cibicidoides wuellerstorfi (Schwager, 1866) from methane seeps and non‐seep habitats on the Pacific margin
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A. M. Burkett A. E. Rathburn M. E. Pérez L. A. Levin H. Cha G. W. Rouse 《Geobiology》2015,13(1):44-52
Benthic foraminifera are among the most abundant groups found in deep‐sea habitats, including methane seep environments. Unlike many groups, no endemic foraminiferal species have been reported from methane seeps, and to our knowledge, genetic data are currently sparse for Pacific deep‐sea foraminifera. In an effort to understand the relationships between seep and non‐seep populations of the deep‐sea foraminifera Cibicidoides wuellerstorfi, a common paleo‐indicator species, specimens from methane seeps in the Pacific were analyzed and compared to one another for genetic similarities of small subunit rDNA (SSU rDNA) sequences. Pacific Ocean C. wuellerstorfi were also compared to those collected from other localities around the world (based on 18S gene available on Genbank, e.g., Schweizer et al., 2009). Results from this study revealed that C. wuellerstorfi living in seeps near Costa Rica and Hydrate Ridge are genetically similar to one another at the species level. Individuals collected from the same location that display opposite coiling directions (dextral and sinstral) had no species level genetic differences. Comparisons of specimens with genetic information available from Genbank (SSU rDNA) showed that Pacific individuals, collected for this study, are genetically similar to those previously analyzed from the North Atlantic and Antarctic. These observations provide strong evidence for the true cosmopolitan nature of C. wuellerstorfi and highlight the importance of understanding how these microscopic organisms are able to maintain sufficient genetic exchange to remain within the same species between seep and non‐seep habitats and over global distances. 相似文献
12.
Molecular Phylogeny and Evolutionary Relationships between the Ciliate Genera Peniculistoma and Mytilophilus (Peniculistomatidae,Pleuronematida)
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Gregory A. Antipa John R. Dolan Denis H. Lynn Lubov A. Obolkina Michaela C. Strüder‐Kypke 《The Journal of eukaryotic microbiology》2016,63(5):642-650
Peniculistoma mytili and Mytilophilus pacificae are placed in the pleuronematid scuticociliate family Peniculistomatidae based on morphology and ecological preference for the mantle cavity of mytiloid bivalves. We tested this placement with sequences of the small subunit rRNA (SSUrRNA) and cytochrome c oxidase subunit 1 (cox1) genes. These species are very closely related sister taxa with no distinct genetic difference in the SSUrRNA sequence but about 21% genetic difference for cox1, supporting their placement together but separation as distinct taxa. Using infection frequencies, M. pacificae, like its sister species P. mytili, does not interact with Ancistrum spp., co‐inhabitants of the mantle cavity. On the basis of these ecological similarities, the fossil record of host mussels, and features of morphology and stomatogenesis of these two ciliates, we argue that M. pacificae derived from a Peniculistoma‐like ancestor after divergence of the two host mussels. Our phylogenetic analyses of pleuronematid ciliates includes the SSUrRNA gene sequence of Sulcigera comosa, a Histiobalantium‐like ciliate from Lake Baikal. We conclude: (i) that the pleuronematids are a monophyletic group; (ii) that the genus Pleuronema is paraphyletic; and (iii) that S. comosa is a Histiobalantium species. We transfer S. comosa to Histiobalantium and propose a new combination Histiobalantium comosa n. comb. 相似文献
13.
Dominique A. Cowart Chunya Huang Sophie Arnaud‐Haond Susan L. Carney Charles R. Fisher Stephen W. Schaeffer 《Molecular ecology》2013,22(16):4147-4162
The history of colonization and dispersal in fauna distributed among deep‐sea chemosynthetic ecosystems remains enigmatic and poorly understood because of an inability to mark and track individuals. A combination of molecular, morphological and environmental data improves understanding of spatial and temporal scales at which panmixia, disruption of gene flow or even speciation may occur. Vestimentiferan tubeworms of the genus Escarpia are important components of deep ‐sea cold seep ecosystems, as they provide long‐term habitat for many other taxa. Three species of Escarpia, Escarpia spicata [Gulf of California (GoC)], Escarpia laminata [Gulf of Mexico (GoM)] and Escarpia southwardae (West African Cold Seeps), have been described based on morphology, but are not discriminated through the use of mitochondrial markers (cytochrome oxidase subunit 1; large ribosomal subunit rDNA, 16S; cytochrome b). Here, we also sequenced the exon‐primed intron‐crossing Haemoglobin subunit B2 intron and genotyped 28 microsatellites to (i) determine the level of genetic differentiation, if any, among the three geographically separated entities and (ii) identify possible population structure at the regional scale within the GoM and West Africa. Results at the global scale support the occurrence of three genetically distinct groups. At the regional scale among eight sampling sites of E. laminata (n = 129) and among three sampling sites of E. southwardae (n = 80), no population structure was detected. These findings suggest that despite the patchiness and isolation of seep habitats, connectivity is high on regional scales. 相似文献
14.
DAVID DUDGEON 《Freshwater Biology》2012,57(9):1794-1812
1. Benthic macroinvertebrate communities were sampled in 30 tributary streams at altitudes from sea level to about 3000 m draining three geologically distinct regions within the catchment of the Sepik River, Papua New Guinea. The fauna of this near‐pristine river has been little studied, and the impacts of ongoing and anticipated human impacts on the Sepik are uncertain. 2. Data on community composition were analysed at different levels of taxonomic resolution (species or morphospecies versus family) to compare their responses to environmental variables such as altitude and geology (reflected in water chemistry), and to indicate their potential utility for the detection of environmental change. 3. A total of 183 000 macroinvertebrates representing 250 species were collected, predominantly insects (232 species and >99% of individuals). The fauna was co‐dominated by Diptera (42% of individuals; 32 morphospecies, mainly Orthocladiinae, Simuliidae and Chironominae) and Ephemeroptera (36%; 48 species), although the Trichoptera showed the highest species (67) and family (13) richness, with Coleoptera ranked third (43 species). Naucoridae (Heteroptera) and Crambidae: Acentropinae (Lepidoptera), each represented by 13 species, were distinctive faunal elements. Mayflies were represented by only four families, one consisting of a single species. 4. Multivariate analysis of the species‐level data set revealed that community composition was influenced by geological region, but the effect was largely due to altitude as most streams in one region (the Central Highlands) were at higher elevations (>800 m) than streams in the other two regions (<500 m). However, altitude had no direct effect on species richness. A secondary influence of current speed and a subsidiary effect of water chemistry (pH and N‐NO3) on community composition were also detected. Naucorid bugs showed evidence of altitudinal zonation and some species replacement, plus a tendency for certain genera to be associated with highland or lowland streams. 5. Analysis of the family‐level data set failed to uncover strong effects of any environmental variable, either individually or in combination, although some sensitivity to altitude plus slope was detected. 6. These findings suggest that attempts to use macroinvertebrates to detect environmental change in New Guinea streams will require species‐level monitoring of community composition. 相似文献
15.
Plot‐to‐plot dissimilarity measures are considered a valuable tool for understanding the complex ecological mechanisms that drive community composition. Traditional presence/absence coefficients are usually based on different combinations of the matching/mismatching components of the 2 × 2 contingency table. However, more recently, dissimilarity measures that incorporate information about the degree of functional differences between the species in both plots have received increasing attention. This is because such “functional dissimilarity measures” capture information on the species' functional traits, which is ignored by traditional coefficients. Therefore, functional dissimilarity measures tend to correlate more strongly with ecosystem‐level processes, as species influence these processes via their traits. In this study, we introduce a new family of dissimilarity measures for presence and absence data, which consider functional dissimilarities among species in the calculation of the matching/mismatching components of the 2 × 2 contingency table. Within this family, the behavior of the Jaccard coefficient, together with its additive components, species replacement, and richness difference, is examined by graphical comparisons and ordinations based on simulated data. 相似文献
16.
17.
Florian T. Wetzel Helmut Beissmann Dustin J. Penn Walter Jetz 《Global Change Biology》2013,19(7):2058-2070
Sea‐level rise (SLR) from global warming may have severe consequences for biodiversity; however, a baseline, broad‐scale assessment of the potential consequences of SLR for island biodiversity is lacking. Here, we quantify area loss for over 12 900 islands and over 3000 terrestrial vertebrates in the Pacific and Southeast Asia under three different SLR scenarios (1 m, 3 m and 6 m). We used very fine‐grained elevation information, which offered >100 times greater spatial detail than previous analyses and allowed us to evaluate thousands of hitherto not assessed small islands. Depending on the SLR scenario, we estimate that 15–62% of islands in our study region will be completely inundated and 19–24% will lose 50–99% of their area. Overall, we project that between 1% and 9% of the total island area in our study region may be lost. We find that Pacific species are 2–3 times more vulnerable than those in the Indomalayan or Australasian region and risk losing 4–22% of range area (1–6 m SLR). Species already listed as threatened by IUCN are particularly vulnerable compared with non‐threatened species. Under a simple area loss–species loss proportionality assumption, we estimate that 37 island group endemic species in this region risk complete inundation of their current global distribution in the 1 m SLR scenario that is widely anticipated for this century (and 118 species under 3 m SLR). Our analysis provides a first, broad‐scale estimate of the potential consequences of SLR for island biodiversity and our findings confirm that islands are extremely vulnerable to sea‐level rise even within this century. 相似文献
18.
Agricultural intensification is altering biodiversity patterns worldwide. Rapid and effective methods are needed to monitor these changes in farmland biodiversity, but it becomes both a cost- and time-prohibitive task, particularly for hyper-diverse groups such as arthropods. We evaluated the effectiveness of surrogates in irrigated and rainfed wheat fields in a Mediterranean farmland in NW Spain in order to get a rapid tool to assess arthropod biodiversity. We studied six groups with different ecological needs (i.e. Aphididae, Aphidiinae, Coccinellidae, Formicidae, Heteroptera and Syrphidae) at species level (147 species), genus (105), family (10, only Heteroptera) and order (19) level. Higher taxa, cross-taxa and subset-taxa or total richness approaches were tested as well as the correlation in composition between levels for the selected groups, and the influence of farming regime. Genus richness was a good surrogate of species richness in all six groups studied (R2 = 0.38–0.60), like family and order were for Heteroptera (R2 = 0.37 and 0.29, respectively). Cross-taxa analyses showed that Aphididae and Aphidiinae genera (R2 = 0.19 and 0.30, respectively) and species (R2 = 0.20 and 0.28, respectively) were good surrogates for Aphidiinae and Aphididae species respectively. Coccinellidae genera (R2 = 0.26) and species (R2 = 0.25) were good surrogates for Heteroptera species. Finally, Aphididae and Coccinellidae both at genera (R2 = 0.14 and 0.20, respectively) and at species levels (R2 = 0.12–0.22, respectively) were good surrogates for total species richness of all groups. Genera composition was the best surrogate for the species composition within each group. Farming regime had no influence on the relationships between surrogates and species patterns in most cases. Our results suggest that genera level is a useful surrogate for all the studied groups and family is appropriate for Heteroptera. Genus level provided a saving of 15% of identification time in Aphididae and 80% for Coccinellidae. This proves its usefulness to asses and monitor biodiversity in wheat croplands and the possibility to reduce costs. 相似文献
19.
Sun Young Kim Dapeng Xu Jae‐Ho Jung Joong Ki Choi 《The Journal of eukaryotic microbiology》2020,67(1):115-124
Six isolates of mineral‐enveloped Strombidinopsis minima‐like species were collected from the coastal waters across several regions in Korea. Morphological observations and molecular analyses were performed. The ribosomal DNA sequences (including small subunit ribosomal DNA, internal transcriber spacer 1‐5.8S ribosomal DNA‐internal transcriber spacer 2; and part of large subunit ribosomal DNA) of these six isolates were compared. Their morphological characteristics were also compared with those of S. minima populations reported. The marked genetic differences (with a similarity range of 96.85–98.48%) in SSU rDNA among these S. minima‐like entities suggest the existence of multiple species. This finding is also supported by morphological variations detected in this study and reported in the literature (e.g. 15–32 collar membranelles in different populations). In addition, S. minima‐like species are clustered with S. batos and S. sinicum, and therefore, our SSU rDNA results support previous results suggesting that the genus Strombidinopsis is not monophyletic in origin. Further collection of morphological and molecular data may facilitate the determination of a new genus carrying mineral‐enveloped Strombidinopsis species. 相似文献
20.
Growth performance and metabolic response of juvenile grouper Epinephelus moara (Temminck & Schlegel, 1842) fed low dietary protein and high lipid levels
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S. M. Peng C. J. Zhang Q. X. Gao Z. H. Shi C. Chen J. G. Wang 《Zeitschrift fur angewandte Ichthyologie》2017,33(4):790-796
Nine test diets were formulated at three low levels of crude protein (P; 35%, 40% or 45%) and three high levels of crude lipid (L; 9%, 12% or 15%) to investigate whether Epinephelus moara differs in the nutrient requirements from any of the other closely‐related grouper species, and if there are any indications that a further decrease in protein with increasing dietary lipid would induce a higher protein‐sparing effect. Fish (20.34 ± 0.82 g/ind) were distributed among 27 experimental cages (100 cm diameter, 80 cm depth), with 20 fish per cage and three replicates for each test diet. The experiment was conducted over an 8‐week period at a temperature of 23.9–25.7°C. Weight gain and specific growth rate (SGR) tended to increase either with an increase in dietary protein level (at the same lipid level) or with the increase in dietary lipid level (at the same protein level, except at the 45% protein level). The highest weight gain and SGR occurred in fish fed the diet P45/L12. The serum cholesterol, high‐density lipoprotein‐cholesterol (HDL‐C) and low‐density lipoprotein‐cholesterol (LDL‐C) contents in fish were significantly increased as the dietary lipid increased. The increase of dietary protein significantly decreased the activities of serum and hepatic alanine aminotransferase (ALT) as well as aspartate aminotransferase (AST). Hepatic fatty acid synthase (FAS) activity significantly increased with the decrease of dietary protein and lipid levels (p < .05). However, lipoprotein lipase (LPL) activity showed an inverse trend compared to FAS. Hepatic glutamate dehydrogenase (GDH) activity significantly increased with the increase in the dietary protein level. In conclusion, the results reveal that growth performance and metabolic responses of E. moara are dependent on dietary protein and lipid levels, and that protein‐sparing action can be induced by increasing the dietary lipid. To support the feeding and growth of juvenile E. moara under the present conditions, 45% protein and 12% lipid are adequate. 相似文献