首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 31 毫秒
1.
The chemical analysis of the neutral extracts of Satureia calamintha and S. graeca aerial parts afforded, besides calaminthadiol, a new triterpene, isocalaminthadiol which belongs to the 3,4-seco-12-ursene class. The isolation of isocalaminthadiol and calaminthadiol may constitute a valid index for the chemotaxonomic characterization of the genus Satureia.  相似文献   

2.
Two new sapogenins, named seco-umbilicagenins A and B (1 and 2), possessing a new chemical structure based on a 3,4-seco-spirostane skeleton, were isolated from Allium umbilicatum Boiss. The chemical structures of seco-umbilicagenins A and B were established through a combination of extensive spectroscopic analysis, mainly nuclear magnetic resonance spectroscopy and mass spectrometry, and chemical methods as (2S,25R)-2,5α,6β-trihydroxy-3,4-seco-spirosta-3,4-dioic acid (1) and (2S,25R)-2,4,5α-trihydroxy-6-oxo-3,4-seco-spirostan-3-oic acid (2). Interestingly, the isolated compounds exhibited cytotoxic activity on J-774, murine monocyte/macrophage, and WEHI-164, murine fibrosarcoma, cell lines. To the best of our knowledge this is the first time that 3,4-seco-spirostane sapogenins are isolated from natural sources, being this skeleton obtained by synthetic modification of intact sapogenins.  相似文献   

3.
4.
A novel lignan dihydroanhydride, (+)-calocedrin, was isolated from the wood of Calocedrus formosana. Its structure was determined to be trans-α-(3,4-methylenedioxybenzylidene)-β-(3,4-methylenedioxybenzyl)-γ-hydroxybutanolide by spectroscopic methods. Reduction of (+)-calocedrin resulted in an optically inactive lignan lactone, (±)-hibalactone.  相似文献   

5.
A novel alkaloid and a galactoside isolated from the roots of Adhatoda vasica have been characterized as 9-acetamido-3,4-dihydropyrido-(3,4-b)-indole and O-ethyl-α-D-galactoside respectively by chemical and spectroscopic methods. In addition sitosterol β-D-glucoside, D-galactose and deoxyvasicinone have also been isolated from the roots of this plant.  相似文献   

6.
A new key is provided to the North American species of sect.Gardoquia; two varieties are recognized in the widespread Mexican speciesS. macrostema; andS. jaliscana, a species associated with barranca-forests in Jalisco, Mexico, is described as new.  相似文献   

7.
《Phytochemistry》1987,26(3):850-852
A new 3,4-seco-ambrosanolide was isolated from Ambrosia artemisiifolia and identified by means of spectroscopic evidence 1H and 13C NMR, IR and MS).  相似文献   

8.
The heartwoods of Acacia giraffae and A. galpinii were selected from South African Acacias as representative of those with abnormally high and minimal tannin contents respectively. A. galpinii contains amongst other analogues, the first natural (+)-2,3-trans-3,4-trans-teracacidin (7,8,4′-trihydroxy-flavan-3,4-diol and novel 3-O-methyl-, 7,8-di-O-methyl- and 7,8,4′-tri-O-methylflavonol analogues. (−)-2,3-cis-3,4-cis-Melacacidin (7,8,3′,4′-tetrahydroxyflavan-3,4-diol) is also present, but tannins are absent. By contrast, from the large excess of leueofisetinidin tannins which characterizes the wood of A. giraffae, only (+)-catechin, (+)-2,3-trans-3,4-trans-leucofisetinidin (7,3′,4′,trihydroxyflavan-3,4-diol and all-trans-(+)-leueofisetinidin-(+)-catechin could be isolated.  相似文献   

9.
Micrococcus strain 12B, grown with phthalate, transformed the phthalate analog, phthalaldehydate (2-formylbenzoate), to 3,4-dihydroxyphthalaldehydate which was isolated and identified as its lactol. An 18O2 incorporation experiment indicated that a dioxygenase mechanism was involved. It is proposed by analogy, that phthalate is metabolized through cis-3,4-dihydro-3,4-dihydroxyphthalate and 3,4-dihydroxyphthalate by this bacterium.  相似文献   

10.
《Phytochemistry》1987,26(12):3225-3227
Five phenolic glycosides were isolated from the leaves of Digitalis purpurea. Four of the glycosides were identified as desrhamnosyl acteoside, forsythiaside, purpureaside A and purpureaside B respectively and the structure of other one was elucidated as 3,4-dihydroxyphenethylalcohol-6-O-caffeoyl- β-D-glucoside. Four phenolic glycoside were isolated from the callus tissue of D. purpurea and identified as purpureaside A, purpureaside B, acteoside and purpureaside C respectively.  相似文献   

11.
3,4-Dihydroxy-2-hydroxymethylpyrrolidine, which has not been encountered naturally before, was isolated from the Pteridophyte Arachniodes standishii. Its configuration was determined as 2,3-cis and 3,4-trans from NMR spectra.  相似文献   

12.
A new natural imino-alcohol, 2,5-dihydroxymethyl 3,4-dihydroxypyrrolidine has been isolated from the leaves of Derris elliptica. Its structure was determined by chemical and physical methods.  相似文献   

13.
The photo-oxidation of cytochrome f (cytochrome c554) in bundle sheath cells isolated from leaves of maize (Zea mays var. DS 606A) has been compared with that in intact maize leaf and in isolated pea leaf cells (Pisum sativum L.). In all cases, illumination with red light caused a negative absorbance change at 554 nm which was attributed to the oxidation of cytochrome f. The extent of this change was greater using monochromatic red light at wavelengths above 700 nm compared with wavelengths below 700 nm. 3-(3,4-Dichlorophenyl)-1, 1-dimethylurea abolished this difference in bundle sheath cells. After illumination for 1 minute or longer in bundle sheath cells, reduction of cytochrome f in the dark was rapid only if the wavelength of the illuminating light was below 700 nm. In the presence of 3-(3,4-dichlorophenyl)-1, 1-dimethlyurea, reduction was slow after illumination at all wavelengths.  相似文献   

14.
Polycyclic aromatic hydrocarbons (PAHs) are metabolized to trans-dihydrodiol proximate carcinogens by CYP1A1 and epoxide hydrolase (EH). CYP1A1 or aldo–keto reductases (AKRs) from the 1C subfamily can further activate the trans-dihydrodiols by forming either anti-diol-epoxides or reactive and redox active o-quinones, respectively. To determine whether other AKR superfamily members can divert trans-dihydrodiols to o-quinones, the cDNA encoding human aldehyde reductase (AKR1A1) was isolated from hepatoma HepG2 cells using RT-PCR, subcloned into a prokaryotic expression vector, overexpressed in E. coli and purified to homogeneity in milligram amounts. Studies revealed that AKR1A1 preferentially oxidized the metabolically relevant (−)-[3R,4R]-dihydroxy-3,4-dihydrobenz[a]anthracene. AKR1A1 also displayed high utilization ratios (Vmax/Km) for the following PAH trans-dihydrodiols: (±)trans-3,4-dihydroxy-3,4-dihydro-7-methylbenz[a]anthracene, (±)trans-3,4-dihydroxy-3,4-dihydro-7,12-dimethylbenz[a]anthracene and (±)trans-7,8-dihydroxy-7,8-dihydro-5-methylchrysene. Multiple tissue expression (MTE) arrays were used to measure the co-expressed of CYP1A1, EH and AKR1A1. All the three enzymes co-expressed to sites of PAH activation. The high catalytic efficiency of AKR1A1 for potent proximate carcinogen trans-dihydrodiols and its presence in tissues that contain CYP1A1 and EH suggests that it plays an important role in this alternative pathway of PAH activation (supported by CA39504).  相似文献   

15.
Phenol is a toxic aromatic compound used or produced in many industries and as a result a common component of industrial wastewaters. Phenol containing waste streams are frequently hypersaline and therefore require halophilic microorganisms for efficient biotreatment without dilution. In this study three halophilic bacteria isolated from different saline environments and identified as Halomonas organivorans, Arhodomonas aquaeolei and Modicisalibacter tunisiensis were shown to be able to grow on phenol in hypersaline media containing 100 g/L of total salts at a concentration of 3 mM (280 mg/L), well above the concentration found in most waste streams. Genes encoding the aromatic dioxygenase enzymes catechol 1,2 dioxygenase and protocatechuate 3,4-dioxygenase were present in all strains as determined by PCR amplification using primers specific for highly conserved regions of the genes. The gene for protocatechuate 3,4-dioxygenase was cloned from the isolated H. organivorans and the translated protein was evaluated by comparative protein sequence analysis with protocatechuate 3,4-dioxygenase proteins from other microorganisms. Although the analysis revealed a wide range of sequence divergence among the protocatechuate 3,4-dioxygenase family, all of the conserved domain amino acid structures identified for this enzyme family are identical or conservatively substituted in the H. organivorans enzyme.  相似文献   

16.
We previously reported two free D-amino acids, D-2-aminopimelic acid (D-APA) and trans-3,4-dehydro-D-2-aminopimelic acid (D-Δ-APA), from Asplenium unilaterale. In the present work we isolated 4-hydroxy-2-aminopimelic acid (OH-APA) from the same plant and determined it to be the α-L-form. We also investigated the configurations of these amino acids isolated from A. prolongatum and A. wilfordii which are morphologically distinct from A. unilaterale. In A. prolongatum, APA was the D- and OH-APA was the L-isomer. In contrast, APA from A. wilfordii was partially racemized and the degree of racemization was significantly different in plant material collected in July and November, L:D = 3:2 and 3:7, respectively. In A. wilfordii OH-APA was almost pure L- and Δ-APA was mostly the D-isomer.  相似文献   

17.
《Phytochemistry》1987,26(7):2057-2061
A new phenylpropanoid glycoside, angoroside A, and a known glycoside, acteoside, were isolated from the roots of Scrophularia scopolii var. scopolii. On the basis of chemical and spectral evidence, angoroside A was shown to be 3,4-dihydroxy-β-phenylethoxy-O-α-l-arabinopyranosyl-(1 → 6)-α-l-rhamnopyranosyl-(1 → 3)-4-O-caffeoyl-β-d-glucopyranoside.  相似文献   

18.
Rhodococcus sp. strain AD45 was isolated from an enrichment culture on isoprene (2-methyl-1,3-butadiene). Isoprene-grown cells of strain AD45 oxidized isoprene to 3,4-epoxy-3-methyl-1-butene, cis-1,2-dichloroethene to cis-1,2-dichloroepoxyethane, and trans-1,2-dichloroethene to trans-1,2-dichloroepoxyethane. Isoprene-grown cells also degraded cis-1,2-dichloroepoxyethane and trans-1,2-dichloroepoxyethane. All organic chlorine was liberated as chloride during degradation of cis-1,2-dichloroepoxyethane. A glutathione (GSH)-dependent activity towards 3,4-epoxy-3-methyl-1-butene, epoxypropane, cis-1,2-dichloroepoxyethane, and trans-1,2-dichloroepoxyethane was detected in cell extracts of cultures grown on isoprene and 3,4-epoxy-3-methyl-1-butene. The epoxide-degrading activity of strain AD45 was irreversibly lost upon incubation of cells with 1,2-epoxyhexane. A conjugate of GSH and 1,2-epoxyhexane was detected in cell extracts of cells exposed to 1,2-epoxyhexane, indicating that GSH is the physiological cofactor of the epoxide-transforming activity. The results indicate that a GSH S-transferase is involved in the metabolism of isoprene and that the enzyme can detoxify reactive epoxides produced by monooxygenation of chlorinated ethenes.  相似文献   

19.
Precocene II (6,7-dimethoxy-2,2-dimethylchromene), the most active anti-juvenile hormone isolated from Ageratum houstonianum, has been shown to be hepatotoxic in male Sprague-Dawley rats. A single 300-mg/kg dose of precocene II administered via i.p. injection caused extensive necrosis of parenchymal cells in the hepatic centrolobular areas. Liver functions were markedly affected as shown by the significant increases of glutamic-oxalacetic transaminase and glutamic-pyruvic transaminase in the serum. By means of reversed-phase high pressure liquid chromatography (HPLC), [3H]precocene II was found to be rapidly metabolized in vitro by rat liver microsomes in an NADPH-generating system. Approximately 5% (3.4 nmol/mg protein) of the radioactivity from the [3H]precocene II substrate was covalently bound to the macromolecular pellet at the end of a 15-min incubation period when phenobarbital (PB)-induced microsomes were used. Results obtained from experiments using different incubation systems indicated the involvement of the cytochrome P-450-dependent monooxygenases in the metabolism of precocene II and the concurrent covalent binding. The most predominent metabolite was isolated and accounted for >90% of the radioactivity associated with the ethylacetate-extractable metabolites. Further analysis by mass spectrometry and proton nuclear magnetic resonance (NMR) spectroscopy identified it as a 37 : 63 stereoisomeric mixture of the cis and trans 3,4-dihydrodiols of precocene II. A highly reactive (3,4-epoxy-6,7-dimethyl-2,2-dimethylchromane (precocene-3,4-epoxide) was thus suggested as a crucial metabolic intermediate which may be responsible for the histopathological changes seen in rat liver.  相似文献   

20.
Screening for microorganisms converting stearic acid to form new compounds was conducted, Pseudomonas cepacia A-1419 isolated from soil effectively produced two compounds showing strong ultraviolet absorption when the resting cells were incubated with stearic acid. The products were isolated, and identified as (Z)-dec-3-ene-1,3,4-tricarboxylic acid 3,4-anhydride (Product 1) and (Z)-dodec-3-ene-1,3,4-tricarboxylic acid 3,4-anhydride (Product 2) by infrared, mass, and nuclear magnetic resonance spectroscopies, and elemental analysis. Products 1 and 2 were produced from stearic acid at conversion rates of about 18 and 32%, respectively.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号