共查询到20条相似文献,搜索用时 78 毫秒
1.
RFLP analysis of soybean seed protein and oil content 总被引:20,自引:0,他引:20
B. W. Diers P. Keim W. R. Fehr R. C. Shoemaker 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,83(5):608-612
Summary The objectives of this study were to present an expanded soybean RFLP map and to identify quantitative trait loci (QTL) in soybean [Glycine max (L.) Merr.] for seed protein and oil content. The study population was formed from a cross between a G. max experimental line (A81-356022) and a G. soja Sieb. and Zucc. plant introduction (PI 468916). A total of 252 markers was mapped in the population, forming 31 linkage groups. Protein and oil content were measured on seed harvested from a replicated trial of 60 F2-derived lines in the F3 generation (F23 lines). Each F23 line was genotyped with 243 RFLP, five isozyme, one storage protein, and three morphological markers. Significant (P<0.01) associations were found between the segregation of markers and seed protein and oil content. Segregation of individual markers explained up to 43% of the total variation for specific traits. All G. max alleles at significant loci for oil content were associated with greater oil content than G. soja alleles. All G. soja alleles at significant loci for protein content were associated with greater protein content than G. max alleles. 相似文献
2.
Blair MW Iriarte G Beebe S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(6):1149-1163
Advanced backcross QTL analysis was used to identify quantitative trait loci (QTL) for agronomic performance in a population
of BC2F3:5 introgression lines created from the cross of a Colombian large red-seeded commercial cultivar, ICA Cerinza, and a wild common
bean accession, G24404. A total of 157 lines were evaluated for phenological traits, plant architecture, seed weight, yield
and yield components in replicated trials in three environments in Colombia and genotyped with microsatellite, SCAR, and phaseolin
markers that were used to create a genetic map that covered all 11 linkage groups of the common bean genome with markers spaced
at an average distance of every 10.4 cM. Segregation distortion was most significant in regions orthologous for a seed coat
color locus (R-C) on linkage group b08 and two domestication syndrome genes, one on linkage group b01 at the determinacy (fin) locus and the other on linkage group b02 at the seed-shattering (st) locus. Composite interval mapping analysis identified a total of 41 significant QTL for the eight traits measured of which
five for seed weight, two for days to flowering, and one for yield were consistent across two or more environments. QTL were
located on every linkage group with b06 showing the greatest number of independent loci. A total of 13 QTL for plant height,
yield and yield components along with a single QTL for seed size showed positive alleles from the wild parent while the remaining
QTL showed positive alleles from the cultivated parent. Some QTL co-localized with regions that had previously been described
to be important for these traits. Compensation was observed between greater pod and seed production and smaller seed size
and may have resulted from QTL for these traits being linked or pleiotropic. Although wild beans have been used before to
transfer biotic stress resistance traits, this study is the first to attempt to simultaneously obtain a higher yield potential
from wild beans and to analyze this trait with single-copy markers. The wild accession was notable for being from a unique
center of diversity and for contributing positive alleles for yield and other traits to the introgression lines showing the
potential that advanced backcrossing has in common bean improvement. 相似文献
3.
Loci underlying resistance to Race 3 of soybean cyst nematode in Glycine soja plant introduction 468916 总被引:6,自引:0,他引:6
D. Wang B. W. Diers P. R. Arelli R. C. Shoemaker 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(4):561-566
Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is an important soybean [Glycine max (L.) Merr.] pest in the U.S. and throughout the world. Genetic resistance is the primary method for controlling SCN and there
is a need to identify new resistance genes. Glycine soja Sieb. and Zucc. is the wild ancestor of domesticated soybean and is a potential source of new SCN resistance genes. The goal
of this research was to map quantitative trait loci (QTLs) that provide resistance to SCN Race 3 from the G. soja plant introduction (PI) 468916. Fifty seven F2-derived lines from a cross between the G. soja PI 468916 and the G. max experimental line A81-356022 were tested for resistance to an SCN population with a Race-3 phenotype. These lines were also
genotyped with 1,004 genetic markers and resistance genes were mapped by composite interval mapping with the computer program
QTL-Cartographer. In the F2 population, three significant (LOD > 3.0) QTLs were detected that explained from 5% to 27% of the variation for Race-3 resistance.
The two most significant QTLs identified in the F2 population were tested in a population of 100 BC1F2 plants developed by crossing A81-356022 to a line from the F2 population that carried the two resistance QTLs from G. soja. In the backcross population, both Race-3 resistance QTLs were significant, which confirms the existence of these QTLs. The
QTLs identified in this experiment map to positions where SCN resistance genes have not been previously identified, suggesting
that these are novel genes that could be useful for diversifying the resistance genes currently used in cultivar development.
Received: 7 August 2000 / Accepted: 4 December 2000 相似文献
4.
QTL affecting fitness of hybrids between wild and cultivated soybeans in experimental fields 总被引:1,自引:0,他引:1
Yosuke Kuroda Akito Kaga Norihiko Tomooka Hiroshi Yano Yoshitake Takada Shin Kato Duncan Vaughan 《Ecology and evolution》2013,3(7):2150-2168
The objective of this study was to identify quantitative trait loci (QTL) affecting fitness of hybrids between wild soybean (Glycine soja) and cultivated soybean (Glycine max). Seed dormancy and seed number, both of which are important for fitness, were evaluated by testing artificial hybrids of G. soja × G. max in a multiple‐site field trial. Generally, the fitness of the F1 hybrids and hybrid derivatives from self‐pollination was lower than that of G. soja due to loss of seed dormancy, whereas the fitness of hybrid derivatives with higher proportions of G. soja genetic background was comparable with that of G. soja. These differences were genetically dissected into QTL for each population. Three QTLs for seed dormancy and one QTL for total seed number were detected in the F2 progenies of two diverse cross combinations. At those four QTLs, the G. max alleles reduced seed number and severely reduced seed survival during the winter, suggesting that major genes acquired during soybean adaptation to cultivation have a selective disadvantage in natural habitats. In progenies with a higher proportion of G. soja genetic background, the genetic effects of the G. max alleles were not expressed as phenotypes because the G. soja alleles were dominant over the G. max alleles. Considering the highly inbreeding nature of these species, most hybrid derivatives would disappear quickly in early self‐pollinating generations in natural habitats because of the low fitness of plants carrying G. max alleles. 相似文献
5.
Deborah Landau-Ellis Sieglinde Angermüller Randy Shoemaker Peter M. Gresshoff 《Molecular & general genetics : MGG》1991,228(1-2):221-226
Summary The genetic locus (nts) controlling nitrate-tolerant nodulation, supernodulation, and diminished autoregulation of nodulation of soybean (Glycine max (L.) Merill) was mapped tightly to the pA-132 molecular marker using a restriction fragment length polymorphism (RFLP) detected by subclone pUTG-132a. The nts (nitrate-tolerant symbiotic) locus of soybean was previously detected after its inactivation by chemical mutagenesis. Mutant plant lines were characterized by abundant nodulation (supernodulation) and tolerance to the inhibitory effects of nitrate on nodule cell proliferation and nitrogen fixation. The large number of RFLPs between G. max line nts382 (homozygous for the recessive nts allele) and the more primitive soybean G. soja (P1468.397) allowed the detection of co-segregation of several diagnostic markers with the supernodulation locus in F2 families. We located the nts locus on the tentative RFLP linkage group E about 10 cM distal to pA-36 and directly next to marker pA-132. This very close linkage of the molecular marker and the nts locus may allow the application of this clone as a diagnostic probe in breeding programs as well as an entry point for the isolation of the nts gene. 相似文献
6.
Buerstmayr M Lemmens M Steiner B Buerstmayr H 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(2):293-306
While many reports on genetic analysis of Fusarium head blight (FHB) resistance in bread wheat have been published during
the past decade, only limited information is available on FHB resistance derived from wheat relatives. In this contribution,
we report on the genetic analysis of FHB resistance derived from Triticum macha (Georgian spelt wheat). As the origin of T. macha is in the Caucasian region, it is supposed that its FHB resistance differs from other well-investigated resistance sources.
To introduce valuable alleles from the landrace T. macha into a modern genetic background, we adopted an advanced backcross QTL mapping scheme. A backcross-derived recombinant-inbred
line population of 321 BC2F3 lines was developed from a cross of T. macha with the Austrian winter wheat cultivar Furore. The population was evaluated for Fusarium resistance in seven field experiments
during four seasons using artificial inoculations. A total of 300 lines of the population were genetically fingerprinted using
SSR and AFLP markers. The resulting linkage map covered 33 linkage groups with 560 markers. Five novel FHB-resistance QTL,
all descending from T. macha, were found on four chromosomes (2A, 2B, 5A, 5B). Several QTL for morphological and developmental traits were mapped in the
same population, which partly overlapped with FHB-resistance QTL. Only the 2BL FHB-resistance QTL co-located with a plant
height QTL. The largest-effect FHB-resistance QTL in this population mapped at the spelt-type locus on chromosome 5A and was
associated with the wild-type allele q, but it is unclear whether q has a pleiotropic effect on FHB resistance or is closely linked to a nearby resistance QTL. 相似文献
7.
QTL associated with horizontal resistance to soybean cyst nematode in Glycine soja PI464925B 总被引:1,自引:0,他引:1
Winter SM Shelp BJ Anderson TR Welacky TW Rajcan I 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(3):461-472
Soybean cyst nematode (Heterodera glycines Ichinohe; SCN) is the primary disease responsible for yield loss of soybean [Glycine max (L.) Merr.]. Resistant cultivars are an effective management tool; however, the sources currently available have common resistant
genes. Glycine soja Sieb. and Zucc., the wild ancestor of domesticated soybean, represents a diverse germplasm pool with known SCN resistance.
The objectives of this research were to: (1) determine the genetic variation and inheritance of SCN resistance in a G. max (‘S08-80’) × G. soja (PI464925B) F
4:5 recombinant inbred line (RIL) population; and (2) identify and evaluate quantitative trait loci (QTL) associated with SCN
resistance. Transgressive segregation for resistance was observed, although neither parent was resistant to the Chatham and
Ruthven SCN isolates. Broad sense heritability was 0.81 for the Ruthven and 0.91 for the Chatham isolate. Root dry weight
was a significant covariate that influenced cyst counts. One RIL [female index (FI) = 5.2 ± 1.11] was identified as resistant
to the Chatham isolate (FI < 10). Seventeen and three RILs infected with Chatham and Ruthven isolates, respectively, had mean
adjusted cyst counts of zero. Unique and novel QTL, which derived resistance from G. soja, were identified on linkage groups I, K, and O, and individually explained 8, 7 and 5% (LOD = 2.1–2.7) of the total phenotypic
variation, respectively. Significant epistatic interactions were found between pairs of SSR markers that individually may
or may not have been associated with SCN resistance, which explained between 10 and 15% of the total phenotypic variation.
Best-fit regression models explained 21 and 31% of the total phenotypic variation in the RIL population to the Chatham and
Ruthven isolates, respectively. The results of this study help to improve the understanding of the genetic control of SCN
resistance in soybean caused by minor genes resulting in horizontal resistance. The incorporation of the novel resistance
QTL from G. soja could increase the durability of SCN-resistance in soybean cultivars, especially if major gene resistance breaks down. 相似文献
8.
Ratnam Wickneswari M. A. R. Bhuiyan Sabu Kalluvettankuzhy K. Li Sze Lim Michael J. Thomson Md. Kairudin Narimah Md. Zain Abdullah 《Plant Molecular Biology Reporter》2012,30(4):929-939
A backcross breeding strategy was used to identify quantitative trait loci (QTLs) associated with 14 traits in a BC2F2 population derived from a cross between MR219, an indica rice cultivar and an accession of Oryza rufipogon (IRGC 105491). A total of 261 lines were genotyped with 96 microsatellite markers and evaluated for plant morphology, yield components and growth period. The genetic linkage map generated for this population with an average interval size of 16.2?cM, spanning 1,553.4?cM (Kosambi) of the rice genome. Thirty-eight QTLs were identified with composite interval mapping (CIM), whereas simple interval mapping (SIM) resulted in 47 QTLs (LOD >3.0). The O. rufipogon allele was favourable for 59% of QTLs detected through CIM. Of 261 BC2F2 families, 26 advanced backcross breeding lines (BC2F5) were used for QTL validation. These lines were selected on the basis of the yield traits potentiality in BC2F3 and BC2F4 generations. The field trial was conducted at three different locations in Malaysia using randomized complete block design with three replications. Trait based marker analysis was done for QTL determination. Twenty-five QTLs were detected in BC2F5 generation whereas 29 QTLs were detected in BC2F2 generation of the same population. Two QTLs (qPL-1 and qSPL-7) were not considered for validation due to their low R 2 values and two QTLs (qPSS-3-2 and qGW-3-2) were not detected in the BC2F5 population. Fifteen QTLs showed the beneficial effect to enhance the trait value of the breeding lines. QTL validation aided to select the promising lines for further utilization. 相似文献
9.
Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading date of rice, as single Mendelian factors 总被引:32,自引:0,他引:32
T. Yamamoto Y. Kuboki S. Y. Lin T. Sasaki M. Yano 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):37-44
Fine mapping was carried out on three putative QTLs (tentatively designated as Hd-1 to Hd-3) of five such QTLs controlling heading date in rice that had been earlier identified using an F2 population derived from a cross between a japonica variety, ‘Nipponbare’, and an indica variety, ‘Kasalath’, using progeny backcrossed with ‘Nipponbare’ as the recurrent parent. One BC3F2 and two BC3F1 plants, in which the target QTL regions were heterozygous and most other chromosomal regions were homozygous for the ‘Nipponbare’
allele, were selected as the experimental material. Self-pollinated progeny (BC3F2 and BC3F3) of the BC3F1 or BC3F2 showed continuous variation in days to heading. By means of progeny testing based on BC3F3 or BC3F4 lines, we determined the genotypes of each BC3F2 or BC3F3 individual at target QTLs. Their segregation patterns fitted Mendelian inheritance ratios. When the results obtained by RFLP
analysis and progeny tests were combined, Hd-1, Hd-2 and Hd-3 were mapped precisely on chromosomes 6, 7 and 6, respectively, of a rice RFLP linkage map. The results demonstrated that
QTLs can be treated as Mendelian factors. Moreover, these precise locations were in good agreement with the regions estimated
by QTL analysis of the initial F2 population, demonstrating the high reliability of QTL mapping using a high-density linkage map.
Received: 5 November 1997 / Accepted: 10 February 1998 相似文献
10.
Identification of quantitative trait loci for plant height, lodging, and maturity in a soybean population segregating for growth habit 总被引:8,自引:0,他引:8
S. H. Lee M. A. Bailey M. A. R. Mian E. R. Shipe D. A. Ashley W. A. Parrott R. S. Hussey H. R. Boerma 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(5):516-523
The use of molecular markers to identify quantitative trait loci (QTLs) has the potential to enhance the efficiency of trait selection in plant breeding. The purpose of the present study was to identify additional QTLs for plant height, lodging, and maturity in a soybean, Glycine max (L.) Merr., population segregating for growth habit. In this study, 153 restriction fragment length polymorphisms (RFLP) and one morphological marker (Dt1) were used to identify QTLs associated with plant height, lodging, and maturity in 111 F2-derived lines from a cross of PI 97100 and Coker 237. The F2-derived lines and two parents were grown at Athens, Ga., and Blackville, S.C., in 1994 and evaluated for phenotypic traits. The genetic linkage map of these 143 loci covered about 1600 cM and converged into 23 linkage groups. Eleven markers remained unlinked. Using interval-mapping analysis for linked markers and single-factor analysis of variance (ANOVA), loci were tested for association with phenotypic data taken at each location as well as mean values over the two locations. In the combined analysis over locations, the major locus associated with plant height was identified as Dt1 on linkage group (LG) L. The Dt1 locus was also associated with lodging. This locus explained 67.7% of the total variation for plant height, and 56.4% for lodging. In addition, two QTLs for plant height (K007 on LG H and A516b on LG N) and one QTL for lodging (cr517 on LG J) were identified. For maturity, two independent QTLs were identified in intervals between R051 and N100, and between B032 and CpTI, on LG K. These QTLs explained 31.2% and 26.2% of the total variation for maturity, respectively. The same QTLs were identified for all traits at each location. This consistency of QTLs may be related to a few QTLs with large effects conditioning plant height, lodging, and maturity in this population. 相似文献
11.
Andru S Pan YB Thongthawee S Burner DM Kimbeng CA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(1):77-93
Tef is a cereal crop of cultural and economic importance in Ethiopia. It is grown primarily for its grain though it is also
an important source of fodder. Tef suffers from lodging that reduces both grain yield and quality. As a first step toward
executing a marker-assisted breeding program for lodging resistance and grain yield improvement, a linkage map was constructed
using 151 F9 recombinant inbred lines obtained by single-seed-descent from a cross between Eragrostis tef and its wild relative Eragrostis pilosa. The map was primarily based on microsatellite (SSR) markers that were developed from SSR-enriched genomic libraries. The
map consisted of 30 linkage groups and spanned a total length of 1,277.4 cM (78.7% of the genome) with an average distance
of 5.7 cM between markers. This is the most saturated map for tef to date, and for the first time, all of the markers are
PCR-based. Using agronomic data from 11 environments and marker data, it was possible to map quantitative trait loci (QTL)
controlling lodging, grain yield and 15 other related traits. The positive effects of the QTL identified from the wild parent
were mainly for earliness, reduced culm length and lodging resistance. In this population, it is now possible to combine lodging
resistance and grain yield using a marker-assisted selection program targeting the QTL identified for both traits. The newly
developed SSR markers will play a key role in germplasm organization, fingerprinting and monitoring the success of the hybridization
process in intra-specific crosses lacking distinctive morphological markers. 相似文献
12.
Robert Vincent J.M. West Marilyn A.L. Inai Shuji Caines Angela Arntzen Lauren Smith Jodee K. St.Clair Dina A. 《Molecular breeding : new strategies in plant improvement》2001,8(3):217-233
Blackmold, caused by the fungus Alternaria alternata, is a major ripe fruit disease of processing tomatoes. Previously, we found blackmold resistance in a wild tomato (Lycopersicon cheesmanii) and quantitative trait loci (QTL) for resistance were mapped in an interspecific population. Five QTLs were selected for introgression from L. cheesmanii into cultivated tomato using marker-assisted selection (MAS). Restriction fragment length polymorphism and PCR-based markers flanking, and within, the chromosomal regions containing QTLs were used for MAS during backcross and selfing generations. BC1 plants heterozygous at the QTLs, and subsequent BC1S1 and BC1S2 lines possessing different homozygous combinations of alleles at the target QTLs, were identified using DNA markers. Field experiments were conducted in 1998 (with 80 marker-selected BC1S2 lines) and 1999 (with 151 marker-selected BC1S2 and BC1S3 lines) at three California locations. Blackmold resistance was assessed during both years, and horticultural traits were evaluated in 1999. The BC1S2 and BC1S3 lines containing L. cheesmanii alleles at the QTLs were associated with a large genetic variance for resistance to blackmold and moderate heritability, suggesting that significant genetic gain may be achieved by selection in this genetic material. L. cheesmanii alleles at three of the five introgressed QTLs showed a significant, positive effect on blackmold resistance. A QTL on chromosome 2 had the largest positive effect on blackmold resistance, alone and in combination with other QTLs, and was also associated with earliness, a positive horticultural trait. The other four QTLs were associated primarily with negative horticultural traits. Fine mapping QTLs using near isogenic lines could help determine if such trait associations are due to linkage drag or pleiotropy. 相似文献
13.
M. A. R. Mian E. R. Shipe J. Alvernaz J. D. Mueller D. A. Ashley H. R. Boerma 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(8):971-974
Soybean, Glycine max (L.) Merr., genotypes are known to differ in chlorimuron ethyl sensitivity (CS). Earlier we have reported two putatively
independent marker loci linked to two quantitative trait loci (QTLs) controlling CS in a soybean population derived from a
cross of PI97100 (sensitive to chlorimuron ethyl) and ‘Coker 237’ (tolerant to chlorimuron ethyl). The objective of the present
study was to quantify the association of the two marker loci with seed yield and related traits in this soybean population
following application of chlorimuron ethyl. Phenotypic data were collected for 111 F2-derived lines of the cross grown in replicated plots at Athens, G.A., in 1994 and 1995, and at Blackville, S.C., in 1995.
The two CS marker loci explained as much as 50% of the genetic variation in seed yield and seed number m-2, but had no association with seed weight, plant height, lodging, seed protein, and seed oil. There were no epistatic interactions
between the two marker loci for any of the traits. The marker locus (cr168-1 on USDA linkage group E) linked to the major
CS QTL explained between 13 and 23% of the variation in seed yield. The Coker 237 allele at this locus was associated with
decreased CS and increased seed yield. The marker locus (Blt015-2 on an unknown linkage group) linked to the minor CS QTL
accounted for a maximum of 11% of the variation in seed yield. The Coker 237 allele at this locus was associated with an increase
in CS and a decrease in seed yield. The association of the two marker loci with seed number m-2 strongly resembled their association with seed yield. Seed yield had a strong positive correlation (r=0.74 – 0.94) with seed number m-2, and the effect of chlorimuron ethyl on seed yield was due mainly to its effect on seed number m-2 rather than seed weight.
Received: 6 August 1996 / Accepted: 28 February 1997 相似文献
14.
Quantitative trait loci for yield and related traits in the wheat population Ning7840 × Clark 总被引:1,自引:0,他引:1
Marza F Bai GH Carver BF Zhou WC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(4):688-698
Grain yield and associated agronomic traits are important factors in wheat (Triticum aestivum L.) improvement. Knowledge regarding the number, genomic location, and effect of quantitative trait loci (QTL) would facilitate
marker-assisted selection and the development of cultivars with desirable characteristics. Our objectives were to identify
QTLs directly and indirectly affecting grain yield expression. A population of 132 F12 recombinant inbred lines (RILs) was derived by single-seed descent from a cross between the Chinese facultative wheat Ning7840
and the US soft red winter wheat Clark. Phenotypic data were collected for 15 yield and other agronomic traits in the RILs
and parental lines from three locations in Oklahoma from 2001 to 2003. Twenty-nine linkage groups, consisting of 363 AFLP
and 47 SSR markers, were identified. Using composite interval mapping (CIM) analysis, 10, 16, 30, and 14 QTLs were detected
for yield, yield components, plant adaptation (shattering and lodging resistance, heading date, and plant height), and spike
morphology traits, respectively. The QTL effects ranged from 7 to 23%. Marker alleles from Clark were associated with a positive
effect for the majority of QTLs for yield and yield components, but gene dispersion was the rule rather than the exception
for this RIL population. Often, QTLs were detected in proximal positions for different traits. Consistent, co-localized QTLs
were identified in linkage groups 1AL, 1B, 4B, 5A, 6A, and 7A, and less consistent but unique QTLs were identified on 2BL,
2BS, 2DL, and 6B. Results of this study provide a benchmark for future efforts on QTL identification for yield traits. 相似文献
15.
Gandhi SD Heesacker AF Freeman CA Argyris J Bradford K Knapp SJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(4):619-629
Wild populations of common sunflower (Helianthus annuus L.) are self-incompatible and have deep seed dormancy, whereas modern cultivars, inbreds, and hybrids are self-compatible and partially-to-strongly self-pollinated, and have shallow seed dormancy. Self-pollination (SP) and seed dormancy are genetically complex traits, the number of self-compatibility (S) loci has been disputed, and none of the putative S loci have been genetically mapped in sunflower. We genetically mapped quantitative trait loci (QTL) for self-incompatibility (SI), SP, and seed dormancy in a backcross population produced from a cross between an elite, self-pollinated, nondormant inbred line (NMS373) and a wild, self-incompatible, dormant population (ANN1811). A population consisting of 212 BC1 progeny was subsequently produced by backcrossing a single hybrid individual to NMS373. BC1 progeny produced 0–838 seeds per primary capitula when naturally selfed and 0–518 seeds per secondary capitula when manually selfed and segregated for a single S locus. The S locus mapped to linkage group 17 and was tightly linked to a cluster of previously identified QTL for several domestication and postdomestication traits. Two synergistically interacting QTL were identified for SP among self-compatible (ss) BC1 progeny (R2=34.6%). NMS373 homozygotes produced 271.5 more seeds per secondary capitulum than heterozygotes. Germination percentages of seeds after-ripened for 4 weeks ranged from 0% to 100% among self-compatible BC1S1 families. Three QTL for seed dormancy were identified (R2=38.3%). QTL effects were in the predicted direction (wild alleles decreased self-pollination and seed germination). The present analysis differentiated between loci governing SI and SP and identified DNA markers for bypassing SI and seed dormancy in elite × wild crosses through marker-assisted selection.Electronic Supplementary Material Electronic supplementary material is available for this article at 相似文献
16.
Fine mapping of a major quantitative trait loci, qSSP7, controlling the number of spikelets per panicle as a single Mendelian factor in rice 总被引:2,自引:0,他引:2
Xing YZ Tang WJ Xue WY Xu CG Zhang Q 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(6):789-796
In our previous studies, one putative QTL affecting number of spikelets per panicle (SPP) was identified in the pericentromeric
region of rice chromosome 7 using a recombinant inbred population. In order to define the QTL (qSPP7), RI50, a recombinant inbred line with 70% of genetic background same as the female parent of Zhenshan 97, was selected to
produce near-isogenic lines for the target region in the present study. In a BC2F2 population consisting of 190 plants, the frequency distribution of SPP was shown to be discontinuous and followed the expected
Mendelian ratios (1:2:1 by progeny test) for single locus segregation. qSPP7 was mapped to a 0.4 cM region between SSR marker RM3859 and RFLP marker C39 based on tests of the BC2F2 population and its progeny. Its additive and dominant effects on SPP were 51.1 and 24.9 spikelets, respectively. Of great
interest, the QTL region also had effects on grain yield per plant (YD), 1,000 grain weight (GW), tillers per plant (TPP)
and seed setting ratio (SR). Significant correlations were observed between SPP and YD (r = 0.66) and between SPP and SR (r = −0.29) in the progeny test. 1082 extremely small panicle plants of a BC3F2 population containing 8,400 individuals were further used to fine map the QTL. It turns out that qSPP7 co-segregated with two markers, RM5436 and RM5499 spanning a physical distance of 912.4 kb. Overall results suggested that
recombination suppression occurred in the region and positional cloning strategy is infeasible for qSPP7 isolation. The higher grain yield of Minghui 63 homozygote as compared to the heterozygote suggested that Minghui 63 homozygote
at qSPP7 in hybrid rice could further improve its yield.
Y. Z. Xing and W. J. Tang contributed equally to this work. 相似文献
17.
Common loci underlie field resistance to soybean sudden death syndrome in Forrest,Pyramid, Essex,and Douglas 总被引:8,自引:0,他引:8
Njiti VN Meksem K Iqbal MJ Johnson JE Kassem MA Zobrist KF Kilo VY Lightfoot DA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(2-3):294-300
Soybean [Glycine max (L.) Merr.] sudden death syndrome (SDS) caused by Fusarium solani f. sp. glycines results in severe yield losses. Resistant cultivars offer the most-effective protection against yield losses but resistant
cultivars such as ’Forrest’ and ’Pyramid’ vary in the nature of their response to SDS. Loci underlying SDS resistance in ’Essex’ × Forrest
are well defined. Our objectives were to identify and characterize loci and alleles that underlie field resistance to SDS
in Pyramid×’Douglas’. SDS disease incidence and disease severity were determined in replicated field trials in six environments
over 4 years. One hundred and twelve polymorphic DNA markers were compared with SDS disease response among 90 recombinant
inbred lines from the cross Pyramid×Douglas. Two quantitative trait loci (QTLs) for resistance to SDS derived their beneficial
alleles from Pyramid, identified on linkage group G by BARC-Satt163 (261-bp allele, P=0.0005, R2=16.0%) and linkage group N by BARC-Satt080 (230-bp allele, P=0.0009, R2=15.6%). Beneficial alleles of both QTLs were previously identified in Forrest. A QTL for re- sistance to SDS on linkage group
C2 identified by BARC-Satt307 (292-bp allele, P=0.0008, R2=13.6%) derived the beneficial allele from Douglas. A beneficial allele of this QTL was previously identified in Essex. Recombinant
inbred lines that carry the beneficial alleles for all three QTLs for resistance to SDS were significantly (P≤0.05) more resistant than other recombinant inbred lines . Among these recombinant inbred lines resistance to SDS was environmentally
stable. Therefore, gene pyramiding will be an effective method for developing cultivars with stable resistance to SDS.
Received: 20 October 1999 / Accepted: 22 May 2001 相似文献
18.
L. M. Mansur J. Orf K. G. Lark 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,86(8):914-918
An experimental test is described for linkages between RFLP markers and quantitative trait loci (QTL). Two hundred and eighty-four F7-derived recombinant inbred lines (RIL) obtained from crossing the soybean cultivars (Glycine max L. Merr.) Minsoy and Noir 1 were evaluated for maturity, plant height, lodging, and seed yield. RIL exhibiting an extreme phenotype for each trait (earliest and latest plants for maturity, etc.) were selected, and two bulked DNA samples were prepared for each trait. A Southern transfer of the digested bulked DNA was hybridized with restriction fragement length polymorphism (RFLP) probes, and linkages with QTL were established by quantitating the amount of radioactive probe that bound to fragments defining alternative parental RFLP alleles. When an RFLP marker was linked to a QTL, one parental allele predominated in the bulked DNA from a particular phenotype; the other allele was associated with the opposite phenotype. When linkage was absent, radioactivity was associated equally with both alleles for a given phenotype (or with both phenotypes for a given allele). These results confirmed RFLP-QTL associations previously discovered by interval mapping on a smaller segregating population from the same cross. New linkages to QTL were also verified. 相似文献
19.
Pozar G Butruille D Silva HD McCuddin ZP Penna JC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(3):553-564
Breeding for resistance to gray leaf spot, caused by Cercospora zeae-maydis (Cz) is paramount for many maize environments, in particular under warm and humid growing conditions. In this study, we mapped
and characterized quantitative trait loci (QTL) involved in the resistance of maize against Cz. We confirmed the impact of
the QTL on disease severity using near-isogenic lines (NILs), and estimated their effects on three major agronomic traits
using their respective near isogenic hybrids (NIHs), which we obtained by crossing the NILs with an inbred from a complementary
heterotic pool. We further validated three of the four QTL that were mapped using the Multiple Interval Mapping approach and
showed LOD values >2.5. NILs genotype included all combinations between favorable alleles of the two QTL located in chromosome
1 (Q
1 in bin 1.05 and Q
2 in bin 1.07), and the allele in chromosome 3 (Q
3 in bin 3.07). Each of the three QTL separately significantly reduced the severity of Cz. However, we found an unfavorable
epistatic interaction between Q
1 and Q
2: presence of the favorable allele at one of the QTL allele effectively nullified the effect of the favorable allele at the
other. In contrast, the interaction between Q
2 and Q
3 was additive, promoting the reduction of the severity to a greater extent than the sum of their individual effects. When
evaluating the NIH we found significant individual effects for Q
1 and Q
3 on gray leaf spot severity, for Q
2 on stalk lodging and grain yield, and for Q
3 on grain moisture and stalk lodging. We detected significant epitasis between Q
1 and Q
2 for grain moisture and between Q
1 and Q
3 for stalk lodging. These results suggest that the combination of QTL impacts the effectiveness of marker-assisted selection
procedures in commercial product development programs. 相似文献
20.
Wang P Zhou G Yu H Yu S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(8):1319-1330
Leaf size is a major determinant of plant architecture and yield potential in crops. A previous study showed that the genomic
region of chromosome 1 contains a major quantitative trait locus (QTL) for flag leaf size in a set of backcross recombinant
inbred lines derived from two elite parental lines (Zhenshan 97 and 93-11). In the present study, the QTL (qFL1) was shown to explain a large proportion of the variation in flag leaf size (leaf length, width and area) in derived populations
(BC2F3 and BC3F2) in multiple environments. Using a large segregating population, we narrowed the location of qFL1 to a 31 kb region containing four predicted genes. Expression of one of these genes, OsFTL1, differed between leaves in near-isogenic lines carrying alleles of Zhenshan 97 and 93-11. qFL1 had a pleiotropic effect on flag leaf size and yield-related traits. Conditional QTL analysis of the derived population (BC3F2) supports the assertion that qFL1 is the QTL for flag leaf length and exhibits pleiotropy. Pyramiding of qFL1 with two known genes (GS3 and Wx) from 93-11 into Zhenshan 97 enlarged flag leaves, improved grain size and amylose content, and increased yield per plant,
but slightly delayed heading date. These results provide a foundation for the functional characterization of the gene underlying
the pleiotropic effects of qFL1 and for genetic improvement of the plant architecture and yield potential of rice. 相似文献