共查询到20条相似文献,搜索用时 125 毫秒
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We have identified and characterized a novel vascular endothelial growth factor (VEGF), VEGF-D, which is structurally related to vascular endothelial growth factor C. A full-length cDNA for human VEGF-D was cloned following the identification of an EST obtained through a TFASTA search of public EST databases. The murine VEGF-D was subsequently isolated from a mouse lung cDNA library. The human VEGF-D gene was mapped to human chromosome Xp22.31. Both human and mouse VEGF-D are strongly expressed in lung and encode the eight cysteine residues that are highly conserved among the members of this family. The high level of conservation between mouse and human VEGF-D may emphasize the biological importance of this gene. Recently the murine gene, FIGF, which is identical to mouse VEGF-D, was reported. 相似文献
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Kira K. Lueders Rosemary W. Elliott Ingo Marenholz Dietmar Mischke Michael DuPree Dean Hamer 《Mammalian genome》1999,10(9):900-905
As a first step in determining whether there are polymorphisms in the nicotinic acetylcholine receptor (nAChR) genes that
are associated with nicotine addiction, we isolated genomic clones of the β2-nAChR genes from human and mouse BAC libraries.
Although cDNA sequences were available for the human gene, only the promoter sequence had been reported for the mouse gene.
We determined the genomic structures by sequencing 12 kb of the human gene and over 7 kb of the mouse gene. While the sizes
of exons in the mouse and human genes are the same, the introns differ in size. Both promoters have a high GC content (60–80%)
proximal to the AUG and share a neural-restrictive silencer element (NRSE), but overall sequence identity is only 72%. Using
a 6-Mb YAC contig of Chr 1, we mapped the human β2-nAChR gene, CHRNB2, to 1q21.3 with the order of markers cen, FLG, IVL,
LOR, CHRNB2, tel. The mouse gene, Acrb2, had previously been mapped to Chr 3 in a region orthologous to human Chr 1. We refined mapping of the mouse gene and other
markers on a radiation hybrid panel of Chr 3 and found the order cen, Acrb2, Lor, Iv1, Flg, tel. Our results indicate that this cluster of markers on human Chr 1 is inverted with respect to its orientation on the
chromosome compared with markers in the orthologous region of mouse Chr 3.
Received: 26 January 1999 / Accepted: 10 May 1999 相似文献
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Hossain MA Bouton CM Pevsner J Laterra J 《The Journal of biological chemistry》2000,275(36):27874-27882
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A 150-kDa glycoprotein designated in the mouse as E-selectin ligand-1 (ESL-1; gene symbol Selel) was first isolated based on its ability to function as a ligand for E-selectin. The gene appears equivalent to that for
membrane glycoprotein MG160 encoded in the human by the locus for Golgi apparatus protein 1 (GLG1). ESL-1 is also highly homologous to the chicken cysteine-rich fibroblast growth factor receptor (CFR). We describe the genomic
structure and chromosomal localization of the Selel locus. The gene is encoded by 27 exons and extends over approximately 75 kb. It maps to murine Chromosome (Chr) 8 in a region
homologous to human Chr 16q where the GLG1 locus maps, further indicating that Selel and GLG1 are mouse and human equivalents of the same gene.
Received: 21 April 1999 / Accepted: 12 July 1999 相似文献
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We have mapped the gene encoding the murine RYK growth factor receptor protein tyrosine kinase by genetic linkage analysis with recombinant inbred strains of mouse. Two distinct Ryk loci (Ryk-1 and Ryk-2) were identified. Ryk-1 mapped to Chromosome (Chr) 9, whereas Ryk-2 mapped to Chr 12. A similar arrangement of RYK-related loci was previously determined in the human. Synteny has already been established between murine Chr 9 in the region of Ryk-1, and human chromosome 3q11–12, the location of the human RYK-1 gene. However, the Ryk-2/RYK-2 loci on murine Chr 12 and human chr 17p13.3 define a new region of synteny. 相似文献
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Zhen Liu Franck Lebrin Janita A. Maring Sander van den Driesche Stieneke van der Brink Maarten van Dinther Midory Thorikay Sabrina Martin Kazuki Kobayashi Lukas J. A. C. Hawinkels Laurens A. van Meeteren Evangelia Pardali Jeroen Korving Michelle Letarte Helen M. Arthur Charles Theuer Marie-José Goumans Christine Mummery Peter ten Dijke 《PloS one》2014,9(1)
ENDOGLIN (ENG) is a co-receptor for transforming growth factor-β (TGF-β) family members that is highly expressed in endothelial cells and has a critical function in the development of the vascular system. Mutations in Eng are associated with the vascular disease known as hereditary hemorrhagic telangiectasia type l. Using mouse embryonic stem cells we observed that angiogenic factors, including vascular endothelial growth factor (VEGF), induce vasculogenesis in embryoid bodies even when Eng deficient cells or cells depleted of Eng using shRNA are used. However, ENG is required for the stem cell-derived endothelial cells to organize effectively into tubular structures. Consistent with this finding, fetal metatarsals isolated from E17.5 Eng heterozygous mouse embryos showed reduced VEGF-induced vascular network formation. Moreover, shRNA-mediated depletion and pharmacological inhibition of ENG in human umbilical vein cells mitigated VEGF-induced angiogenesis. In summary, we demonstrate that ENG is required for efficient VEGF-induced angiogenesis. 相似文献
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Martin L. Katz Po-Ching Liu Sarah E. Grob-Nunn Hisashi Shibuya Gary S. Johnson 《Mammalian genome》1999,10(11):1050-1053
Late-infantile ceroid-lipofuscinosis (CLN2) is an autosomal recessively inherited, neurodegenerative disease in humans. The
CLN2 locus has been mapped to Chromosome (Chr) 11p15, and its sequence and genomic organization have recently been reported. In
the present study, the cDNA sequence, exon/intron organization, and chromosomal localization of a mouse ortholog of the CLN2 gene are described. The mouse cDNA contains an open reading frame that predicts a protein product of 562 amino acids. The
mouse and human coding regions are 86% and 88% identical at the nucleic acid and amino acid levels, respectively. One less
codon appears in the mouse cDNA when compared with the human ortholog. The mouse gene (Cln2) spans more than 6 kb and consists of 13 exons separated by introns ranging in size from 111 to 1259 bp. Length polymorphism
in an (AC)n microsatellite in intron 3 of the mouse Cln2 gene was used to perform segregation analysis with The Jackson Laboratory DNA Panel Mapping Resource. On the basis of this
analysis, the Cln2 gene was localized to a region of mouse Chr 7 that corresponds to human Chr 11p15. Characterization of the mouse Cln2 gene will facilitate generation of a mouse model for late-infantile ceroid-lipofuscinosis by gene targeting and identification
of functionally important regions of the Cln2 protein.
Received: 25 May 1999 / Accepted: 22 July 1999 相似文献
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AIDS-associated Kaposi's sarcoma cells in culture express vascular endothelial growth factor. 总被引:12,自引:0,他引:12
K Weindel D Marmé H A Weich 《Biochemical and biophysical research communications》1992,183(3):1167-1174
PCR cloning and cDNA sequencing have been used to identify mRNAs of two splice products of the vascular endothelial growth factor (VEGF) gene, VEGF121 and VEGF165, in cells isolated from Kaposi's sarcomas (KS) of AIDS patients (AIDS-KS). As demonstrated by Northern blot analysis, AIDS-KS cells as well as tumor cells show a high expression level of the VEGF gene as compared to primary human vascular cells like smooth muscle cells or endothelial cells. In addition to the lower expression of the gene, vascular cells express a 3.9 kb band together with a 3.2 kb band instead of a 3.9 kb and a 4.3 kb band in AIDS-KS cells. Our data suggest that the angiogenic properties of AIDS-KS cells might be mediated by the secretion of this growth factor and that this factor alone or in combination with other endothelial mitogens may be involved in endothelial proliferation associated with Kaposi's sarcoma. 相似文献
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J. Michael Salbaum 《Mammalian genome》1999,10(2):107-111
The mouse gene Punc encodes a member of the immunoglobulin superfamily of cell surface proteins. It is highly expressed in the developing embryo
in nervous system and limb buds. At mid-gestation, however, expression levels of Punc decrease sharply. To allow investigation of such a regulatory mechanism, the genomic locus encompassing the Punc gene was cloned, characterized, and mapped. Fluorescent in situ hybridization was used to determine the chromosomal location
of the Punc gene of mouse and human. Mouse Punc maps to Chromosome (Chr) 9 in the region D-E1, whereas the human PUNC gene is localized to Chr 15 at 15q22.3-23, a region
known to be syntenic to mouse 9D-E1. The human PUNC gene therefore maps close to a genetic locus that is linked to Bardet-Biedl
Syndrome, an autosomal recessive human disorder. Confirmation for the location of human PUNC was obtained through sequence
relationships between mouse Punc cDNA, human PUNC cDNA, genomic sequence upstream of the murine Punc gene, and human STS markers that had been previously mapped on Chr 15. The STS sequence WI-14920 is in fact derived from
the 3′-untranslated region of the human PUNC gene. WI-14920 had been placed at 228cR from the top of the Chr 15 linkage group,
which provided positional information for the human PUNC gene at high resolution. Thus, this study identifies PUNC as the
gene corresponding to a previously anonymous marker and serves as a basis to investigate its role in genetic disorders.
Received: 8 July 1998 / Accepted: 14 October 1998 相似文献