Evaluation of methods for cultivating limbal mesenchymal stromal cells

Background aimsMesenchymal stromal cells (MSC) with similar properties to bone marrow-derived mesenchymal stromal cells (BM-MSC) have recently been grown from the limbus of the human cornea. We have evaluated methods for culturing human limbal MSC (L-MSC).MethodsFour basic strategies were compared: serum-supplemented medium (10% fetal bovine serum; FBS), standard serum-free medium supplemented with B-27, epidermal growth factor and fibroblast growth factor 2, or one of two commercial serum-free media, defined keratinocyte serum-free medium (Invitrogen) and MesenCult-XF® (Stem Cell Technologies). The resulting cultures were examined using photography, flow cytometry (for CD34, CD45, CD73, CD90, CD105, CD141 and CD271), immunocytochemistry (alpha-smooth muscle actin; α-sma), differentiation assays (osteogenesis, adipogenesis and chrondrogenesis) and co-culture experiments with human limbal epithelial (HLE) cells.ResultsWhile all techniques supported the establishment of cultures to varying degrees, sustained growth and serial propagation were only achieved in 10% FBS medium or MesenCult-XF medium. Cultures established in 10% FBS medium were 70–80% CD34^? CD45^? CD90⁺ CD73⁺ CD105⁺, approximately 25% α-sma⁺ and displayed multipotency. Cultures established in MesenCult-XF were > 95% CD34^? CD45^? CD90⁺ CD73⁺ CD105⁺, 40% CD141⁺, rarely expressed α-sma, and displayed multipotency. L-MSC supported growth of HLE cells, with the largest epithelial islands being observed in the presence of MesenCult-XF-grown L-MSC. All HLE cultures supported by L-MSC widely expressed the progenitor cell marker ?Np63, along with the corneal differentiation marker cytokeratin 3.ConclusionsMesenCult-XF is a superior culture system for L-MSC, but further studies are required to explore the significance of CD141 expression in these cells.     

孔腔镜肺叶切除及淋巴结清扫术围手术期应用不同剂量氨溴索对肺癌患者的作用研究

摘要 目的：探讨围手术期应用不同剂量氨溴索对行单孔腔镜肺叶切除及淋巴结清扫术的肺癌患者的作用。方法：选取本院2017年1月至2018年12月期间收治的124例肺癌患者作为受试者,结合患者意愿按随机数字表法将受试者分为小剂量组（n=41）、中剂量组（n=41）和大剂量组（n=42）,术前1d开始给予氨溴索静脉滴注,术后持续7d,比较各组患者临床症状、治疗前后的肺功能、炎症因子、T细胞亚群水平变化及不良反应发生率。结果：大剂量组咳痰容易人数所占比例高于小剂量组,痰液稀薄人数所占比例高于中剂量组及小剂量组（P<0.05）。治疗后大剂量组第一秒用力呼吸容积（FEV¹）、FEV¹/用力肺活量（FVC）高于中剂量组、小剂量组（P<0.05）。治疗后各组炎症因子水平均高于治疗前,且随着剂量的升高,C反应蛋白（CRP）、白介素-6（IL-6）、白介素-8（IL-8）水平呈降低的趋势（P<0.05）。随着剂量的升高,治疗后的 CD3⁺、CD4⁺、CD4⁺/CD8⁺均呈升高趋势,CD8⁺呈降低趋势（P<0.05）。各组不良反应发生率比较无统计学差异（P>0.05）。结论：肺癌患者行单孔腔镜肺叶切除及淋巴结清扫术围手术期应用120mg氨溴索,可加快患者术后恢复,保护患者肺功能,抑制炎症反应,改善患者的免疫功能和临床症状。     

结直肠癌围术期营养干预对患者术后营养指标、T淋巴细胞亚群及并发症的影响

目的:探讨结直肠癌围术期营养干预对患者术后营养指标、T淋巴细胞亚群及并发症的影响。方法:选取2017年3月～2019年3月期间我院收治的结直肠癌患者103例,根据随机数字表法将患者分为对照组(n=51)和研究组(n=52),对照组给予常规方案干预,研究组给予营养支持方案干预。比较两组围术期指标、营养指标、T淋巴细胞亚群及并发症。结果:研究组患者的排便时间、首次肛门排气时间、住院时间均短于对照组(P0.05)。对照组术后1 d、术后7 d的CD3+、CD4~+、CD4~+/CD8~+均低于入院时,CD8~+则高于入院时(P0.05);研究组术后1 d、术后7 d的CD3~+、CD4~+、CD4~+/CD8~+呈先降低后升高趋势,CD8~+呈先升高后降低趋势(P0.05);研究组术后1 d、术后7 d的CD3+、CD4~+、CD4~+/CD8~+高于对照组,CD8~+则低于对照组(P0.05)。两组术后1 d、术后7 d血清前清蛋白(PA)、血红蛋白(Hb)、总蛋白(TP)、转铁蛋白(TF)水平均呈先下降后升高趋势(P0.05);研究组术后1 d、术后7 d血清PA、Hb、TP、TF水平均高于对照组(P0.05)。两组并发症发生率比较差异无统计学意义(P0.05)。结论:结直肠癌围术期给予营养支持方案干预,可有效改善患者营养状态,提高免疫功能,且不增加并发症发生率,具有一定的临床应用价值。     

Serum cobalamin in children with moderate acute malnutrition in Burkina Faso: Secondary analysis of a randomized trial

BackgroundAmong children with moderate acute malnutrition (MAM) the level of serum cobalamin (SC) and effect of food supplements are unknown. We aimed to assess prevalence and correlates of low SC in children with MAM, associations with hemoglobin and development, and effects of food supplements on SC.Methods and findingsA randomized 2 × 2 × 3 factorial trial was conducted in Burkina Faso. Children aged 6 to 23 months with MAM received 500 kcal/d as lipid-based nutrient supplement (LNS) or corn–soy blend (CSB), containing dehulled soy (DS) or soy isolate (SI) and 0%, 20%, or 50% of total protein from milk for 3 months. Randomization resulted in baseline equivalence between intervention groups. Data on hemoglobin and development were available at baseline. SC was available at baseline and after 3 and 6 months. SC was available from 1,192 (74.1%) of 1,609 children at baseline. The mean (±SD) age was 12.6 (±5.0) months, and 54% were females. Low mid-upper arm circumference (MUAC; <125 mm) was found in 80.4% (958) of the children and low weight-for-length z-score (WLZ; <−2) in 70.6% (841). Stunting was seen in 38.2% (456). Only 5.9% were not breastfed. Median (IQR) SC was 188 (137; 259) pmol/L. Two-thirds had SC ≤222 pmol/L, which was associated with lower hemoglobin. After age and sex adjustments, very low SC (<112 pmol/L) was associated with 0.21 (95% CI: 0.01; 0.41, p = 0.04) and 0.24 (95% CI: 0.06; 0.42, p = 0.01) z-score lower fine and gross motor development, respectively.SC data were available from 1,330 (85.9%) of 1,548 children followed up after 3 months and 398 (26.5%) of the 1,503 children after 6 months. Based on tobit regression, accounting for left censored data, and adjustments for correlates of missing data, the mean (95% CI) increments in SC from baseline to the 3- and 6-month follow-up were 72 (65; 79, p < 0.001) and 26 (16; 37, p < 0.001) pmol/L, respectively. The changes were similar among the 310 children with SC data at all 3 time points. Yet, the increase was 39 (20; 57, p < 0.001) pmol/L larger in children given LNS compared to CSB if based on SI (interaction, p < 0.001). No effect of milk was found. Four children died, and no child developed an allergic reaction to supplements. The main limitation of this study was that only SC was available as a marker of status and was missing from a quarter of the children.ConclusionsLow SC is prevalent among children with MAM and may contribute to impaired erythropoiesis and child development. The SC increase during supplementation was inadequate. The bioavailability and adequacy of cobalamin in food supplements should be reconsidered.Trial registrationISRCTN Registry ISRCTN42569496.

Henrik Friis and colleagues, investigate the prevalence and correlates of low serum cobalamin, associations with hemoglobin and child development, and associations of serum cobalamin levels with food supplementation in children moderate acute malnutrition in Burkina Faso.     

腹腔镜与开腹胃楔形切除术对GIST患者营养状况、T细胞亚群和预后的影响

摘要 目的：探讨腹腔镜与开腹胃楔形切除术对胃间质瘤（GIST）患者营养状况、T细胞亚群和预后的影响。方法：回顾性分析2013年1月~2014年2月期间我院收治的GIST患者97例的临床资料,根据手术方式的不同将患者分为对照组（n=47,开腹胃楔形切除术）和研究组（n=50,腹腔镜胃楔形切除术）,对比两组患者手术相关指标、营养状况、T细胞亚群、预后情况。结果：研究组住院时间、肛门排气时间、切口长度、手术时间短于对照组,术中出血量少于对照组（P<0.05）。两组患者术后7 d前白蛋白、转铁蛋白、白蛋白均降低,但研究组高于对照组（P<0.05）。两组术后7 d的 CD4⁺/CD8⁺、CD3⁺、CD4⁺较术前下降,CD8+较术前升高（P<0.05）;研究组术后7 d 的CD4⁺/CD8⁺、CD3⁺、CD4⁺高于对照组,CD8⁺则低于对照组（P<0.05）。研究组并发症总发生率低于对照组（P<0.05）;两组术后复发率、5年生存率比较无统计学差异（P>0.05）。结论：与开腹胃楔形切除术相比,GIST患者采用腹腔镜胃楔形切除术治疗的远期预后效果相当,可有效改善围术期相关指标,对患者营养状况、T细胞亚群的影响较轻,且可减少术后并发症的发生率。     

加速康复外科对腹腔镜胃癌根治术患者营养状态,免疫功能及炎性因子水平的影响

目的:探讨加速康复外科(ERAS)对腹腔镜胃癌根治术患者营养状态,免疫功能及炎性因子水平的影响。方法:将2016年1月~2020年1月于我院行腹腔镜胃癌根治术的胃癌患者166例纳入本研究,按照随机数字表法分为ERAS组(n=83)与对照组(n=83),对照组行常规处理,ERAS组以ERAS处理。观察两组术后1 d、7 d血红蛋白(Hb)、视黄醇结合蛋白(RbP)、转铁蛋白(TRF)、前白蛋白(PRE)、白蛋白(ALB)等营养指标,免疫球蛋白A(Ig A)、免疫球蛋白G(Ig G)、免疫球蛋白M(Ig M)等免疫球蛋白,CD3⁺、CD4⁺、CD8⁺、CD4⁺/CD8⁺等T细胞亚群,白介素-6(IL-6)、白介素-8(IL-8)、白介素-10(IL-10)等炎性因子及术后并发症等指标。结果:与术后1 d比较,两组术后7 d Hb、RbP、TRF、PRE、ALB、Ig A、Ig G、Ig M,CD3⁺、CD4⁺、CD4⁺/CD8⁺水平均升高,CD8⁺、IL-6、IL-8、IL-10水平均降低(P<0.05)。术后7 d,ERAS组Hb、RbP、TRF、PRE、ALB、Ig A、Ig G、Ig M、CD3⁺、CD4⁺、CD4⁺/CD8⁺水平均高于对照组,CD8⁺、IL-6、IL-8、IL-10低于对照组(P<0.05)。ERAS组术后并发症发病率(8.43%)低于对照组(22.89%),差异有统计学意义(P<0.05)。结论:ERAS应用于腹腔镜胃癌根治术中可有效降低患者机体应激反应,改善营养状态,解除免疫抑制,清除炎性因子,减少术后并发症,有助于患者康复。     

温补肾阳法治疗肾阳虚模型大鼠多尿症状的机制研究

摘要 目的：探讨温补肾阳法治疗肾阳虚模型大鼠多尿症状的作用机制。方法：90只雄性SD大鼠随机分成干预组、抑制剂组、空白组、模型组,干预组根据中药剂量分为高剂量组、中剂量组、低剂量组。模型组、干预组及抑制剂组接受肾阳虚模型制备,干预组在成模后每日接受7 g/kg、14 g/kg、28 g/kg剂量中药灌胃,连续灌胃14 d。抑制剂组大鼠接受尾静脉注射通路抑制剂H-89。干预结束后比较各组脏器指数、24 h尿量、24 h尿蛋白水平以及尿液钠离子（Na⁺）、钾离子（K⁺）、氯离子（Cl^-）浓度、肾脏病理变化、血清醛固酮（ALD）、乙醇脱氢酶（ADH）、促肾上腺皮质激素释放因子（CRF）、大鼠促肾上腺皮质激素（ACTH）、大鼠皮质醇（CORT）、蛋白激酶A（PKA）、蛋白激酶A（cAMP）含量、肾脏组织水通道蛋白2（AQP-2）蛋白表达的变化。结果：温补肾阳法可明显减少肾阳虚模型大鼠尿量,改善临床症状,且具有一定的剂量依赖性（P<0.05）。经过中药干预后大鼠24 h尿蛋白、脏器指数、尿液Na⁺、Cl^-均下降,尿液K⁺、血清ALD、ADH、CRF、ACTH、CORT、PKA、cAMP含量、肾脏组织AQP-2蛋白表达上调（P<0.05）,且抑制剂H-89可阻断该作用。结论：温补肾阳法可明显改善肾阳虚模型大鼠多尿症状,其作用机制可能通过cAMP-PKA-AQP2通路介导。     

贮存式自体成分输血对胃肠肿瘤根治术患者T淋巴细胞亚群、血液流变学和预后的影响

摘要 目的：研究贮存式自体成分输血对胃肠肿瘤根治术患者T淋巴细胞亚群、血液流变学以及预后的影响。方法：选取2016年12月~2017年12月我院收治的95例行胃肠肿瘤根治术的胃肠肿瘤早期患者作为研究对象。按随机数表法分为A组（n=47,贮存式自体成分输血）和B组(n=48,异体成分输血)。比较两组患者血常规指标[红细胞计数(RBC)、血红蛋白（Hb）、血细胞比容（Hct）]、免疫功能指标（CD3⁺、CD4⁺、CD8⁺、CD4⁺/CD8⁺）、血液流变学指标[红细胞沉降率、平均血液黏度、红细胞刚性指数及红细胞变形指数]的变化。随访患者2年,采用Kaplan-Meier曲线分析两组预后情况。结果：术前至术后7 d 时间段A组的CD3⁺、CD4⁺水平及CD4⁺/CD8⁺呈先降低后升高趋势,且术后7 d已恢复至术前水平;B组术后1 d、3 d、7 d的CD3⁺、CD4⁺水平及CD4⁺/CD8⁺明显低于A组(P＜0.05),两组CD8⁺水平相比无差异(P＞0.05) 。两组术后7 d的RBC、Hct、Hb分别较输血前下降（P＜0.05）,但两组间比较差异无统计学意义(P＞0.05);两组患者术后7 d的红细胞沉降率均升高,但A组低于B组(P＜0.05) ,两组患者术后的红细胞变形指数、平均血液黏度和红细胞刚性指数比较无差异 (P＞0.05) 。Kaplan-Meier检验结果显示,A组较B组生存率明显升高（P＜0.05）。结论：贮存式自体成分输血对胃肠肿瘤根治术患者红细胞沉降率、细胞免疫功能的影响程度较异体成分输血减轻,且贮存式自体成分输血可以明显提高患者的术后生存率。     

电子支气管镜下氩气刀联合冷冻疗法治疗晚期肺癌的临床疗效及对患者癌性疼痛和免疫功能的影响

目的:评价电子支气管镜下氩气刀联合冷冻疗法治疗晚期肺癌的疗效及对患者癌性疼痛和免疫功能的影响。方法:选择我院2016年7月～2018年7月收治的94例晚期肺癌患者,按随机数字表法分为对照组(51例)和4研究组(43例)。对照组采用常规化疗,研究组在对照组基础上联合电子支气管镜下氩气刀和冷冻疗法。治疗后,比较两组的临床疗效,治疗前后疼痛程度、疼痛介质、免疫功能、生活质量的变化和不良反应的发生情况。结果:治疗后,研究组疾病控制率显著高于对照组(P0.05),两组疼痛Ⅰ级率、CD4~+、CD4~+/CD8~+及生活质量评分均较治疗前显著上升(P0.05),而疼痛介质及CD8~+均较治疗前明显下降(P0.05),研究组疼痛Ⅰ级率、CD4~+、CD4+/CD8~+及生活质量评分明显高于对照组,而疼痛介质及CD8~+均显著低于对照组(P0.05)。两组总不良反应发生率比较差异无统计学意义(P0.05)。结论:电子支气管镜下氩气刀联合冷冻疗法用于治疗晚期肺癌的疗效明显优于常规化疗,其可显著减轻癌性疼痛,改善患者免疫功能斌提高患者的生活质量。     

齐墩果酸通过调控STAT3通路改善咪喹莫特诱导的银屑病样小鼠皮损表现

摘要 目的：观察齐墩果酸对咪喹莫特诱导的银屑病样小鼠的皮损及STAT3通路的干预作用。方法：72只BALB/c雄性小鼠,按随机数字表法随机分为空白组、模型组、甲氨蝶呤组、齐墩果酸低、中、高剂量组,每组12只,于背部备皮。除空白组外,其余各组小鼠每日于背部备皮区域涂抹5%咪喹莫特乳膏诱导银屑病样皮损;甲氨蝶呤组及齐墩果酸各浓度组每日灌胃对应药物0.2 mL/只;每日观察皮损状态并拍照记录,每日对小鼠皮损面积及严重程度（Psoriasis Area and Severity Index,PASI）进行评分;造模第4、7天取小鼠皮损区域皮肤,免疫印迹法检测皮损中STAT3信号通路中STAT3磷酸化表达情况;第7天称量小鼠体重及脾重,计算脾指数,同时取小鼠皮损区域皮肤;苏木素-伊红（HE）染色观察皮损组织病理学改变,并测量表皮厚度;免疫组织化学法及免疫组织荧光法检测皮损中CD3⁺、CD4⁺、CD8⁺T细胞浸润情况及Ki67表达情况;实时荧光定量PCR法检测皮损中白介素-17（Interleukin-17,IL-17）mRNA相对表达情况。结果：与空白组相比,造模后模型组小鼠背部皮肤出现红斑、鳞屑、浸润性皮损,第4天及第7天PASI评分升高（P<0.01）;第7天模型组表皮厚度明显增加,体重下降,脾指数上升,皮损中Ki67、CD3⁺、CD4+⁺、CD8⁺T细胞表达量升高,皮损中IL-17的mRNA相对表达量升高（均P<0.01）;第4及第7天,模型组小鼠皮损中p-STAT3的表达量均明显升高（P<0.05,P<0.01）;与模型组相比,第4天各治疗组在PASI评分略降低（P>0.05）,第7天各治疗组PASI评分明显降低（均P<0.01）;第7天齐墩果酸各剂量组小鼠体重略有上升（P>0.05）;甲氨蝶呤组小鼠脾指数明显下降（P<0.01）,齐墩果酸低、中剂量组小鼠脾指数略下降（P>0.05）;各治疗组小鼠表皮厚度较模型组明显下降（P<0.01或P<0.05）,小鼠皮肤中Ki-67表达量、CD4⁺、CD8⁺T细胞表达量均明显降低（均P<0.01）;甲氨蝶呤组、齐墩果酸低、中、高剂量组小鼠皮损中CD3⁺T细胞表达量均下降（P<0.05,P>0.05,P<0.01,P<0.01）,其中在齐墩果酸各剂量组中,中剂量组在脾指数下降、表皮厚度变薄、CD3⁺、CD4⁺、CD8⁺T细胞减少方面均略优于低、高剂量组（P>0.05）;甲氨蝶呤组及齐墩果酸中剂量组小鼠皮损中IL-17的mRNA水平较模型组明显下降（P<0.01,P<0.05）;造模给药第4天,甲氨蝶呤组小鼠皮损中p-STAT3水平较模型组明显下降（P<0.05）,齐墩果酸中剂量组略下降（P>0.05）;造模给药第7天,两个治疗组小鼠皮损中p-STAT3水平较模型组均明显下降（均P<0.01）。结论：齐墩果酸低、中、高剂量均可以改善咪喹莫特诱导的小鼠银屑病样皮损的严重程度,减轻炎性细胞浸润,中剂量组疗效较好,其机制可能通过抑制STAT3通路中p-STAT3的表达从而降低了IL-17的含量,且药效随作用时间的延长而逐渐增加。     

脓毒症患者血清HMGB1、IGF-1水平变化及与T淋巴细胞亚群、预后的关系研究

目的:探讨脓毒症患者血清高迁移率族蛋白1(HMGB1)、胰岛素样生长因子-1(IGF-1)水平变化及与T淋巴细胞亚群、预后的关系。方法:选取2016年2月～2018年12月期间我院收治的脓毒症患者139例,根据Sepsis 3.0定义,将脓毒症患者分成一般脓毒症组(n=73)及脓毒症休克组(n=66),根据患者进入重症监护室28d后的转归资料,将其分为存活组和死亡组。比较不同预后、不同病情严重程度的脓毒症患者血清IGF-1、HMGB1水平、急性病生理与慢性健康评价系统Ⅱ(APACHEⅡ)评分以及T淋巴细胞亚群;采用Pearson相关分析血清HMGB1、IGF-1水平与T淋巴细胞亚群、APACHEⅡ评分的关系。结果:一般脓毒症组CD3~+、CD4~+、CD4~+/CD8~+高于脓毒症休克组,CD8~+低于脓毒症休克组(P0.05)。脓毒症休克组血清HMGB1水平、APACHEⅡ评分均高于一般脓毒症组,血清IGF-1水平则低于一般脓毒症组(P0.05)。存活组CD8~+低于死亡组,CD3~+、CD4~+、CD4~+/CD8~+高于死亡组(P0.05)。存活组血清HMGB1水平、APACHEⅡ评分低于死亡组,血清IGF-1水平高于死亡组(P0.05)。Pearson相关分析显示,脓毒症患者血清HMGB1水平与CD8~+、APACHEⅡ评分呈正相关,与CD3~+、CD4~+、CD4~+/CD8~+呈负相关(P0.05);血清IGF-1水平与CD8~+、APACHEⅡ评分呈负相关,与CD3~+、CD4~+、CD4~+/CD8~+呈正相关(P0.05)。结论:脓毒症血清HMGB1、T淋巴细胞亚群、IGF-1均存在异常变化,可用于评估脓毒症患者的病情和预后。     

Immunosuppressive properties of mesenchymal stromal cell cultures derived from the limbus of human and rabbit corneas

Background aimsMesenchymal stromal cells (MSCs) cultivated from the corneal limbus (L-MSCs) provide a potential source of cells for corneal repair. In the present study, we investigated the immunosuppressive properties of human L-MSCs and putative rabbit L-MSCs to develop an allogeneic therapy and animal model of L-MSC transplantation.MethodsMSC-like cultures were established from the limbal stroma of human and rabbit (New Zealand white) corneas using either serum-supplemented medium or a commercial serum-free MSC medium (MesenCult-XF Culture Kit; Stem Cell Technologies, Melbourne, Australia). L-MSC phenotype was examined by flow cytometry. The immunosuppressive properties of L-MSC cultures were assessed using mixed leukocyte reactions. L-MSC cultures were also tested for their ability to support colony formation by primary limbal epithelial (LE) cells.ResultsHuman L-MSC cultures were typically CD34⁻, CD45⁻ and HLA-DR⁻ and CD73⁺, CD90⁺, CD105⁺ and HLA-ABC⁺. High levels (>80%) of CD146 expression were observed for L-MSC cultures grown in serum-supplemented medium but not cultures grown in MesenCult-XF (approximately 1%). Rabbit L-MSCs were approximately 95% positive for major histocompatibility complex class I and expressed lower levels of major histocompatibility complex class II (approximately 10%), CD45 (approximately 20%), CD105 (approximately 60%) and CD90 (<10%). Human L-MSCs and rabbit L-MSCs suppressed human T-cell proliferation by up to 75%. Conversely, L-MSCs from either species stimulated a 2-fold to 3-fold increase in LE cell colony formation.ConclusionsL-MSCs display immunosuppressive qualities in addition to their established non-immunogenic profile and stimulate LE cell growth in vitro across species boundaries. These results support the potential use of allogeneic L-MSCs in the treatment of corneal disorders and suggest that the rabbit would provide a useful pre-clinical model.     

不同剂量朱砂七总蒽醌对H22荷瘤小鼠免疫功能及抗氧化能力的影响

目的:探讨不同剂量朱砂七总蒽醌对H22荷瘤小鼠免疫功能及抗氧化能力的影响。方法:选取清洁级昆明小鼠60只,按照随机数字表法分为正常对照组、H22荷瘤组、环磷酰胺组、低剂量组、中剂量组、高剂量组,每组各10只。除正常对照组外,其余5组小鼠建立H22荷瘤小鼠模型。低剂量组、中剂量组、高剂量组分别给予0.3 g/kg、0.6 g/kg、1.2 g/kg的朱砂七总蒽醌悬浊液干预,环磷酰胺组给予0.02 g/kg的环磷酰胺干预,正常对照组和H22荷瘤组给予等剂量的1%的羧甲基纤维素钠干预。比较各组小鼠的肿瘤体质量、抑瘤率、T淋巴细胞亚群以及血清超氧化歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、乳酸脱氢酶(LDH)水平。结果:环磷酰胺组、高剂量组的肿瘤体质量低于低剂量组,抑瘤率高于低剂量组,差异具有统计学意义(P0.05)。高剂量组的CD4+、CD4+/CD8+均高于环磷酰胺组、低剂量组、中剂量组,CD8+低于环磷酰胺组、低剂量组、中剂量组,差异均有统计学意义(P0.05)。高剂量组血清SOD、GSH-Px水平高于其他5组,MDA、LDH水平低于H22荷瘤组、低剂量组、中剂量组,差异均有统计学意义(P0.05)。结论:朱砂七总蒽醌具有明显的抗肿瘤作用,可增强小鼠的免疫功能和抗氧化能力,且具有剂量效应。     

顺铂联合VEGF抗体对食管癌移植瘤小鼠免疫调节以及癌细胞增殖和肺转移的影响

摘要 目的：研究顺铂联合血管内皮生长因子（vascular endothelial growth factor,VEGF）治疗对食管癌移植瘤小鼠免疫功能、癌细胞增殖以及肺转移的影响。方法：30只BALB/c小鼠通过皮下注射食管癌移植瘤模型。一周后,30只食管癌移植瘤模型小鼠被随机均分为3组,即模型组、顺铂组和联合组。模型组不进行治疗,顺铂组腹腔注顺铂治疗,联合组腹腔注射顺铂联合尾静脉注射VEGF抗体进行治疗,共治疗7周。比较各组小鼠体重,食管癌移植瘤体积和重量,卵巢癌细胞肺组织转移结节数、癌细胞转移面积和转移病灶总数,以及食管癌移植瘤外周血CD4⁺、CD8⁺以及CD4⁺/CD8⁺ T淋巴细胞比例。结果：（1）顺博组和联合组小鼠体重均显著高于对照组,而联合组小鼠体重显著高于顺铂组（P<0.05）;（2）顺铂组和联合组小鼠CD4+和CD8+细胞比例均显著低于对照组（P<0.05）,而CD4⁺/CD8⁺却显著高于对照组（P<0.05）;（3）联合组小鼠CD4⁺和CD8⁺细胞比例均显著高于对照组（P<0.05）,而CD4⁺/CD8⁺却显著低于顺铂组（P<0.05）;（4）顺铂组和联合组小鼠食管癌肿瘤组织体积和重量,肺转移结节数、转移面积和转移病灶数均显著低于对照组（P<0.05）,而联合组小鼠显著低于顺铂组（P<0.05）。结论：VEGF抗体可以显著增强顺铂在体内对食管癌的抗癌特性,并有助于增强食管癌移植瘤小鼠免疫功能、抑制癌细胞体内增殖和肺部转移。     

地佐辛联合舒芬太尼对开胸患者术后镇痛镇静效果、血流动力学及免疫功能的影响

目的:探讨地佐辛联合舒芬太尼对开胸患者术后镇痛镇静效果、血流动力学及免疫功能的影响。方法:选取2017年1月~2018年12月期间新疆医科大学附属第一医院收治的拟行开胸手术患者70例,根据术后镇痛药物的不同将患者分为对照组(n=35)和研究组(n=35),对照组术后给予舒芬太尼镇痛,研究组术后给予地佐辛联合舒芬太尼镇痛。比较两组患者镇痛镇静效果、血流动力学、免疫功能及不良反应发生情况。结果:研究组术后6 h、12 h、24 h视觉疼痛模拟评分法(VAS)评分呈逐渐递减趋势,且低于对照组(P<0.05)。研究组术后3 h、6 h、12 h、24 h Ramsay镇静评分均低于对照组(P<0.05)。研究组术后5 min、3 h、12 h心率(HR)、舒张压(DBP)、收缩压(SBP)均低于对照组(P<0.05)。两组患者术后1 d、7 d CD3^+、CD4^+/CD8^+、自然杀伤(NK)细胞水平均呈先降低后升高趋势,术后7 d可恢复至术前水平(P<0.05),研究组术后1 d CD3^+、CD4^+/CD8^+、NK细胞水平高于对照组(P<0.05)。研究组不良反应发生率低于对照组(P<0.05)。结论:开胸患者术后应用地佐辛联合舒芬太尼,镇静镇痛效果较好,可有效维持患者血流动力学稳定,减轻机体免疫功能抑制,安全可靠。     

New multiple labelling method for improved satellite cell identification in human muscle: application to a cohort of power-lifters and sedentary men

Presently applied methods to identify and quantify human satellite cells (SCs) give discrepant results. We introduce a new immunofluorescence method that simultaneously monitors two SC markers (NCAM and Pax7), the basal lamina and nuclei. Biopsies from power-lifters, power-lifters using anabolic substances and untrained subjects were re-examined. Significantly different results from those with staining for NCAM and nuclei were observed. There were three subtypes of SCs; NCAM⁺/Pax7⁺ (94%), NCAM⁺/Pax7⁻ (4%) and NCAM⁻/Pax7⁺ (1%) but large individual variability existed. The proportion of SCs per nuclei within the basal lamina of myofibres (SC/N) was similar for all groups reflecting a balance between the number of SCs and myonuclei to maintain homeostasis. We emphasise that it is important to quantify both SC/N and the number of SCs per fibre. Our multiple marker method is more reliable for SC identification and quantification and can be used to evaluate other markers of muscle progenitor cells.     

Serum Factors Inhibit Melanoma Cell Surface Expression of Type I and Type II IGF Receptors

Abstract

We have identified one class of IGF-I-binding sites and two classes of IGF-II-binding sites at the surface of the melanoma cell line IGR39. By means of affinity labeling with ¹²⁵I-IGF-I, a 290–300 kDa form was characterized. Using ¹²⁵I-IGF-II, a 270 kDa polypeptide was labeled, corresponding to the type II IGF receptor. In the two serials of experiments, the order of potency in inhibiting 125I-IGF-I or ¹²⁵I-IGF-II labeling of IGF-related peptides and αIR3, an antibody directed against type I receptor α subunit, was the same as in competition experiments. When IGR39 cells were cultured in a serum-free medium, the number of both high affinity IGF-II and IGF-I binding sites was increased, by 8-and 5-fold respectively, without any significant change in K_d values. In both culture conditions, we found IGFBP-2, -3, -4 and a 30 kDa form which Mr was consistent with IGFBP-5 or -6. Except for IGFBP-2, the amount of secreted IGFBPs was modified depending on culture conditions: in conditioned medium from cells cultured with 10% FCS, the amount of IGFBP-3 or -4 was higher, and the amount of the 30 kDa IGFBP was lower when compared to conditioned medium from cells cultured in serum-free medium.     

复可托对恶性肿瘤患者免疫功能、生活质量影响的临床观察

目的:观察复可托对恶性肿瘤患者免疫功能、生活质量的影响。方法:将117例恶性肿瘤患者随机分为治疗组(60例)和对照组(57例),对照组给予最佳支持治疗,治疗组在此基础上给予复可托治疗连续6个月。观察并比较两组治疗前、治疗后2、4、6个月的外周血T淋巴细胞亚群的变化及生活质量评分。结果:治疗2个月后,治疗组CD3~+、CD4~+细胞比例、CD4~+/CD8~+较对照组显著升高(P0.05),CD8~+细胞比例较治疗前下降,但差异无统计学意义(P0.05);治疗4、6个月后,治疗组CD3~+、CD4~+细胞比例、CD4~+/CD8~+升高,CD8~+细胞比例下降,差异有统计学意义(P0.05);治疗6个月后,治疗组的KPS评分改善有效率和体重改善有效率均显著高于对照组,差异有统计学意义(P0.05)。两组均未见明显不良反应发生。结论:复可托能纠正恶性肿瘤患者外周血T淋巴细胞亚群功能紊乱,同时明显改善患者生活质量,减少体重下降,且安全性高。     

T淋巴细胞亚群、血红蛋白及血小板在类风湿关节炎患者中的表达及临床意义分析

摘要 目的：探讨T淋巴细胞亚群、血红蛋白及血小板在类风湿关节炎患者中的表达及临床意义。方法：选取我院2020年1月到2023年1月收治的100例类风湿关节炎患者作为研究对象,依照患者病情活动性进行分组,将活动期类风湿关节炎的35例患者分为活动期组,将65例缓解期类风湿关节炎患者分为缓解期组,另选取同期体检的50名健康志愿者作为对照组,对比三组患者CD3⁺、CD4⁺、CD8⁺以及CD4⁺/CD8⁺比值,并对比三组受检者血红蛋白及血小板表达水平。应用Spearman相关分析分析T淋巴细胞亚群、血红蛋白及血小板与类风湿关节炎活动程度的相关性,并应用logistic回归分析分析T淋巴细胞亚群、血红蛋白及血小板对类风湿关节炎活动期的独立预测价值。结果：三组受检者T淋巴细胞亚群表达水平对比有差异,且活动期组CD3⁺、CD4⁺、CD4⁺/CD8⁺水平较缓解期组和对照组低,CD8⁺水平较高（P＜0.05）;三组受检者血红蛋白及血小板表达水平对比差异显著,且活动期组血红蛋白水平较缓解期组和对照组低,血小板水平较高（P＜0.05）;Spearman相关分析结果显示：CD3⁺、CD4⁺、CD4⁺/CD8⁺、血红蛋白与类风湿关节炎病情活动程度呈负相关,CD8⁺、血小板与类风湿关节炎病情活动程度呈正相关（P＜0.05）;logistic回归分析结果表明：CD4⁺/CD8⁺升高、血红蛋白升高及血小板降低为类风湿关节炎活动期的独立影响因素（P＜0.05）。结论：类风湿关节炎患者在疾病活动期T淋巴细胞亚群相关细胞比例、血红蛋白及血小板表达水平会出现明显变化,且与其活动程度具有明显相关性。以CD4⁺/CD8⁺升高、血红蛋白升高及血小板降低情况可独立判定类风湿关节炎活动期,因此临床上对于上述指标升高的类风湿关节炎患者需及时改善治疗措施,改善患者预后水平。