共查询到20条相似文献,搜索用时 31 毫秒
1.
Sompong Chankaew Prakit Somta Worawit Sorajjapinun Peerasak Srinives 《Molecular breeding : new strategies in plant improvement》2011,28(2):255-264
Cercospora leaf spot (CLS) caused by the fungus Cercospora canescens Illis & Martin is a serious disease in mungbean (Vigna radiata (L.) Wilczek), and disease can reduce seed yield by up to 50%. We report here for the first time quantitative trait loci
(QTL) mapping for CLS resistance in mungbean. The QTL analysis was conducted using F2 (KPS1 × V4718) and BC1F1 [(KPS1 × V4718) × KPS1] populations developed from crosses between the CLS-resistant mungbean V4718 and CLS-susceptible cultivar
Kamphaeng Saen 1 (KPS1). CLS resistance in F2 populations was evaluated under field conditions during the wet seasons of 2008 and 2009, and resistance in BC1F1 was evaluated under field conditions during the wet season in 2008. Seven hundred and fifty-three simple sequence repeat
(SSR) markers from various legumes were used to assess polymorphism between KPS1 and V4718. Subsequently, 69 polymorphic markers
were analyzed in the F2 and BC1F1 populations. The results of segregation analysis indicated that resistance to CLS is controlled by a single dominant gene,
while composite interval mapping consistently identified one major QTL (qCLS) for CLS resistance on linkage group 3 in both F2 and BC1F1 populations. qCLS was located between markers CEDG117 and VR393, and accounted for 65.5–80.53% of the disease score variation depending on
seasons and populations. An allele from V4718 increased the resistance. The SSR markers flanking qCLS will facilitate transferral of the CLS resistance allele from V4718 into elite mungbean cultivars. 相似文献
2.
An approach for developing codominant polymorphic markers (compound microsatellite (SSR) markers), with substantial time and
cost savings, is introduced in this paper. In this technique, fragments flanked by a compound SSR sequence at one end were
amplified from the constructed DNA library using compound SSR primer (AC)6(AG)5 or (TC)6(AC)5 and an adaptor primer for the suppression-PCR. A locus-specific primer was designed from the sequence flanking the compound
SSR. The primer pairs of the locus-specific and compound SSR primers were used as a compound SSR marker. Because only one
locus-specific primer was needed for design of each marker and only a common compound SSR primer was needed as the fluorescence-labeled
primer for analyzing all the compound SSR markers, this approach substantially reduced the cost of developing codominant markers
and analyzing their polymorphism. We have demonstrated this technique for Dendropanax trifidus and easily developed 11 codominant markers with high polymorphism for D. trifidus. Use of the technique for successful isolation of codominant compound SSR markers for several other plant species is currently
in progress. 相似文献
3.
Construction of BAC and BIBAC libraries and their applications for generation of SSR markers for genome analysis of chickpea, Cicer arietinum L. 总被引:3,自引:0,他引:3
Lichtenzveig J Scheuring C Dodge J Abbo S Zhang HB 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,110(3):492-510
Large-insert bacterial artificial chromosome (BAC) libraries, plant-transformation-competent binary BAC (BIBAC) libraries, and simple sequence repeat (SSR) markers are essential for many aspects of genomics research. We constructed a BAC library and a BIBAC library from the nuclear DNA of chickpea, Cicer arietinum L., cv. Hadas, partially digested with HindIII and BamHI, respectively. The BAC library has 14,976 clones, with an average insert size of 121 kb, and the BIBAC library consists of 23,040 clones, with an average insert size of 145 kb. The combined libraries collectively cover ca. 7.0× genomes of chickpea. We screened the BAC library with eight synthetic SSR oligos, (GA)10, (GAA)7, (AT)10, (TAA)7, (TGA)7, (CA)10, (CAA)7, and (CCA)7. Positive BACs were selected, subcloned, and sequenced for SSR marker development. Two hundred and thirty-three new chickpea SSR markers were developed and characterized by PCR, using chickpea DNA as template. These results have demonstrated that BACs are an excellent source for SSR marker development in chickpea. We also estimated the distribution of the SSR loci in the chickpea genome. The SSR motifs (TAA)n and (GA)n were much more abundant than the others, and the distribution of the SSR loci appeared non-random. The BAC and BIBAC libraries and new SSR markers will provide valuable resources for chickpea genomics research and breeding (the libraries and their filters are available to the public at ).J. Lichtenzveig and C. Scheuring contributed equally to this study. 相似文献
4.
Ana Rosa de Campos Vaz Tereza Cristina de Oliveira Borba Claudio Brondani Paulo Hideo Nakano Rangel Graziela Silvia de Oliveira Camargo Mariana Pires de Campos Telles José Alexandre Felizola Diniz Filho Rosana Pereira Vianello Brondani 《Genetica》2009,137(2):221-231
Knowledge of natural diversity and population structures of wild species, which might be related to cultivated species, is
fundamental for conservation and breeding purposes. In this study, a genetic characterization of a large population of Oryza glumaepatula, occurring in a 10 km2 area located at Tamengo Basin (Paraguay River, Brazil), was performed using SSR markers. This population is annually dragged
from the river to permit navigation; one goal of this study was to examine the impact of this removal on genetic variability.
From 18 polymorphic SSR markers, a total of 190 alleles were detected in a sample of 126 individuals, with an average of 10.3 alleles/locus,
and a H
e of 0.67. The five QTL-related markers showed an average H
e value of 0.56, while the remaining 13 markers detected an average estimate of 0.70. An apparent outcrossing rate of 30%,
a high proportion of alleles at low frequencies (56%), and the presence of exclusive alleles (9.5%) were found, with strong
evidence of the establishment of individuals from different populations upstream in the Paraguay River. For conservation purposes,
the river drag has no effect on the population. However, periodical seed collection from the Corumbá population can preserve
part of the genetic variability present in upstream populations reducing the need for upriver collecting expeditions. 相似文献
5.
Iniguez-Luy FL Voort AV Osborn TC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(6):977-985
The traditional development of simple sequence repeat (SSR) or microsatellite markers by probe hybridization can be time-consuming
and requires the use of specialized laboratory equipment. In this study, probe hybridization was circumvented by using sequence
information on 3,500 genomic clones mainly from Brassica oleracea to identify di, tri, tetra and penta-nucleotide repeats. A total of 587 primer pairs flanking SSR were developed using this
approach. From these, 420 SSR markers amplified DNA in two parental lines of B. rapa (26% were polymorphic) and 523 in two parental lines of B. oleracea (32% were polymorphic). A diverse array of motif types was identified, characterized and compared with traditional SSR detection
methods. The most abundant motifs found were di- (38%) and trinucleotides (33%) followed by penta- (16%) and tetranucleotide
(13%) motifs. The type of motif class, motif length and repeat were not indicative of polymorphisms. The frequency of B. oleracea SSRs in genomic shotgun sequence was estimated to be 1 every 4 Kb. In general, the average motif length and repeat numbers
were shorter than those obtained previously by probe hybridization, and they contained a more balanced representation of SSR
motif types in the genome by identifying those that do not hybridize well to DNA probes. Brassica genomic DNA sequence information is a promising resource for developing a large number of SSR molecular markers in Brassica species.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
6.
Osman A. Gutiérrez Arin F. Robinson Johnie N. Jenkins Jack C. McCarty Martin J. Wubben Franklin E. Callahan Robert L. Nichols 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(2):271-280
The identification of molecular markers that are closely linked to gene(s) in Gossypium barbadense L. accession GB713 that confer a high level of resistance to reniform nematode (RN), Rotylenchulus reniformis Linford & Oliveira, would be very useful in cotton breeding programs. Our objectives were to determine the inheritance of
RN resistance in the accession GB713, to identify SSR markers linked with RN resistance QTLs, and to map these linked markers
to specific chromosomes. We grew and scored plants for RN reproduction in the P1, P2, F1, F2, BC1P1, and BC1P2 generations from the cross of GB713 × Acala Nem-X. The generation means analysis using the six generations indicated that
one or more genes were involved in the RN resistance of GB713. The interspecific F2 population of 300 plants was genotyped with SSR molecular markers that covered most of the chromosomes of Upland cotton (G. hirsutum L.). Results showed two QTLs on chromosome 21 and one QTL on chromosome 18. One QTL on chromosome 21 was at map position
168.6 (LOD 28.0) flanked by SSR markers, BNL 1551_162 and GH 132_199 at positions 154.2 and 177.3, respectively. A second
QTL on chromosome 21 was at map position 182.7 (LOD 24.6) flanked by SSR markers BNL 4011_155 and BNL 3279_106 at positions
180.6 and 184.5, respectively. Our chromosome 21 map had 61 SSR markers covering 219 cM. One QTL with smaller genetic effects
was localized to chromosome 18 at map position 39.6 (LOD 4.0) and flanked by SSR markers BNL 1721_178 and BNL 569_131 at positions
27.6 and 42.9, respectively. The two QTLs on chromosome 21 had significant additive and dominance effects, which were about
equal for each QTL. The QTL on chromosome 18 showed larger additive than dominance effects. Following the precedent set by
the naming of the G. longicalyx Hutchinson & Lee and G. aridum [(Rose & Standley) Skovsted] sources of resistance, we suggest the usage of Ren
barb1
and Ren
barb2
to designate these QTLs on chromosome 21 and Ren
barb3
on chromosome 18. 相似文献
7.
Cassava (Manihot esculenta) is an economically important crop that is grown in tropical and sub-tropical regions. Use of molecular technology for genetic
improvement of cassava has been limited by the lack of a large set of DNA markers and a genetic map. Therefore, the aims here
were to develop additional simple sequence repeat (SSR) markers from the public expressed sequence tags (ESTs), and to construct
a genetic linkage map. In this study, we designed 425 EST-SSR markers from sequences obtained from the cassava EST database
in GenBank, and integrated them with 667 SSR markers from a microsatellite-enriched genomic sequence received from the International
Center for Tropical Agriculture (CIAT). Of these, 107 EST-SSR and 500 genomic SSR primer pairs showed polymorphic patterns
when screened in two cassava varieties, Hauy Bong 60 and Hanatee, which were used as female and male parental lines, respectively.
Within the 107 and 500 primer pairs, 81 and 226 EST-SSR and SSR primer pairs were successfully genotyped with 100 samples
of F1 progeny, respectively. The results showed 20 linkage groups consisting of 211 markers—56 EST-SSR and 155 SSR markers—spanning
1,178 cM, with an average distance between markers of 5.6 cM and about 11 markers per linkage group. These novel EST-SSR markers
provided genic PCR-based co-dominant markers that were useful, reliable and economical. The EST-SSRs were used together with
SSR markers to construct the cassava genetic linkage map which will be useful for the identification of quantitative trait
loci controlling the traits of interest in cassava breeding programs. 相似文献
8.
Li Y Yang L Pathak M Li D He X Weng Y 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(6):973-983
The compact (dwarf) plant architecture is an important trait in cucumber (Cucumis sativus L.) breeding that has the potential to be used in once-over mechanical harvest of cucumber production. Compact growth habit
is controlled by a simply inherited recessive gene cp. With 150 F2:3 families derived from two inbred cucumber lines, PI 308915 (compact vining) and PI 249561 (regular vining), we conducted
genome-wide molecular mapping with microsatellite (simple sequence repeat, SSR) markers. A framework genetic map was constructed
consisting of 187 SSR loci in seven linkage groups (chromosomes) covering 527.5 cM. Linkage analysis placed cp at the distal half of the long arm of cucumber Chromosome 4. Molecular markers cosegregating with the cp locus were identified through whole genome scaffold-based chromosome walking. Fine genetic mapping with 1,269 F2 plants delimited the cp locus to a 220 kb genomic DNA region. Annotation and function prediction of genes in this region identified a homolog of
the cytokinin oxidase (CKX) gene, which may be a potential candidate of compact gene. Alignment of the CKX gene homologs from both parental lines revealed a 3-bp deletion in the first exon of PI 308915,
which can serve as a marker for marker-assisted selection of the compact phenotype. This work also provides a solid foundation
for map-based cloning of the compact gene and understanding the molecular mechanisms of the dwarfing in cucumber. 相似文献
9.
The sacred lotus (Nelumbo nucifera Gaertn.) is an aquatic plant of economic and ornamental importance in China. In this study, we developed twenty novel sacred
lotus SSR markers, and used AFLP and SSR markers to investigate the genetic diversity and genetic relationships among 58 accessions
of N.
nucifera including 15 seed lotus, 12 rhizome lotus, 24 flower lotus and 7 wild lotus. Our results showed that sacred lotus exhibited
a low level of genetic diversity, which may attribute to asexual reproduction and long-term artificial selection. A dendrogram
based on both AFLP and SSR clustering data showed that: (1) the seed lotus accessions and rhizome lotus accessions were distinctly
clustered into different groups, which indicated the significant genetic differentiation between them. This may be attributed
to the two modes of reproduction and lack of genetic exchange; (2) the accessions of Thailand wild lotus were separated from
other wild lotus accessions. This implied that the Thailand lotus might be genetically differentiated from other wild lotuses.
In addition, Mantel test conducted gave highly significant correlation between AFLP-SSR data and each of the AFLP and SSR
ones, with the values of r = 0.941 and r = 0.879, respectively, indicating the higher efficiency of the combination of these techniques (AFLP and SSR) in estimation
and validation of the genetic diversity among the accession of sacred lotus. This knowledge of the genetic diversity and genetic
relatedness of N. nucifera is potentially useful to improve the current strategies in breeding and germplasm conservation to enhance the ornamental
and economic value of sacred lotus. 相似文献
10.
Peng HF Zhang ZF Wu B Chen XH Zhang GQ Zhang ZM Wan BH Lu YP 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,118(1):77-83
The reverse photoperiod-sensitive genic male sterility (PGMS) and thermo-sensitive genic male sterility (TGMS) lines have
an opposite phenotype compared with normal PGMS and TGMS lines widely used by the two-line system in current hybrid rice seed
production. Thus, the application of reverse PGMS and TGMS lines can compensate PGMS and TGMS lines in hybrid rice production.
YiD1S is a reverse PGMS line, in which pollen fertility is mainly regulated by day-length, but also influenced by temperature.
Genetic analysis indicated that male sterility of YiD1S was controlled by two recessive major genes. An F2 population from a cross between YiD1S and 8528 was developed and used for molecular mapping of the two reverse PGMS genes
which were first named rpms1 and rpms2. Both simple sequence repeat (SSR) markers and bulked segregant analysis (BSA) were used in this study. As a result, one
reverse PGMS gene (rpms1) was mapped to the interval between SSR markers RM22980 (0.9 cM) and RM23017 (1.8 cM) on chromosome 8. Eight SSR markers,
YDS818, RM22984, RM22986, RM22997, YDS816, RM23002, RM339 and YDS810 completely co-segregated with the rpms1 gene. Another reverse PGMS gene (rpms2) was mapped to the interval between SSR markers RM23898 (0.9 cM) and YDS926 (0.9 cM) on chromosome 9. The physical mapping
information from publicly available resources shows that the rpms1 and rpms2 loci are located in a region of 998 and 68 kb, respectively. The analysis based on marker genotypes showed that the effect
of rpms1 was slightly larger than that of rpms2 and that the two genes interacted in controlling male sterility.
H. F. Peng, Z. F. Zhang and B. Wu contributed equally to this work. 相似文献
11.
Rai R Singh AK Singh BD Joshi AK Chand R Srivastava CP 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(5):803-813
Pea rust caused by Uromyces fabae (Pers.) de-Bary is a major problem in warm humid regions causing huge economic losses. A mapping population of 136 F6:7 recombinant inbred lines (RILs) derived from the cross between pea genotypes, HUVP 1 (susceptible) and FC 1 (resistant) was
evaluated in polyhouse as well as under field conditions during two consecutive years. Infection frequency (IF) and area under
disease progress curve (AUDPC) were used for evaluation of rust reaction of the RILs. A linkage map was constructed with 57
polymorphic loci selected from 148 simple sequence repeats (SSRs), 3 sequence tagged sites (STS), and 2 random amplified polymorphic
(RAPD) markers covering 634 cM of genetic distance on the seven linkage groups of pea with an average interval length of 11.3 cM.
Composite interval mapping (CIM) revealed one major (Qruf) and one minor (Qruf1) QTL for rust resistance on LGVII. The LOD (5.2–15.8) peak for Qruf was flanked by SSR markers, AA505 and AA446 (10.8 cM), explaining 22.2–42.4% and 23.5–58.8% of the total phenotypic variation
for IF and AUDPC, respectively. The minor QTL was environment-specific, and it was detected only in the polyhouse (LOD values
4.2 and 4.8). It was flanked by SSR markers, AD146 and AA416 (7.3 cM), and explained 11.2–12.4% of the total phenotypic variation.
The major QTL Qruf was consistently identified across all the four environments. Therefore, the SSR markers flanking Qruf would be useful for marker-assisted selection for pea rust (U. fabae) resistance. 相似文献
12.
M. Luciana Rosso Sarah A. Burleson Laura M. Maupin Katy Martin Rainey 《Molecular breeding : new strategies in plant improvement》2011,28(1):127-132
Mutations in the d-myo-inositol 3-phosphate synthase 1 gene (MIPS1) in soybean [Glycine max (L.) Merr.] cause modifications to seed phosphorus and carbohydrate content that improve the nutritional value of food and
feed. Molecular markers are an efficient tool for breeding MIPS1 mutant germplasm due to reduced seed germination and field emergence potential. An F2 population segregating for the MIPS1 mutation found in experimental soybean line V99-5089 was used to develop breeder-friendly markers. Markers were validated
in 88 advanced lines from 9 diverse pedigrees. Ten potential simple sequence repeat (SSR) markers, located on Gm11, from the
new BARCSOYSSR_1.0 database were tested and four were polymorphic. BARCSOY_11_1495 was 93–97% effective for selecting the
mutation. A KBiosciences Competitive Allele Specific PCR (KASPar) assay was developed to select directly for the V99-5089-derived
MIPS1 single nucleotide polymorphism (SNP) mutation. The KASPar assay is simple and cost-effective compared to other SNP genotyping
assays. The MIPS1 mutation in V99-5089 is likely to have occurred spontaneously. We describe a method of DNA extraction in soybean using a
Geno/Grinder for fast and easy tissue maceration. 相似文献
13.
Kyung-Ho Ma Nam-Soo Kim Gi-An Lee Sok-Young Lee Ju Kyong Lee Jung Yoon Yi Yong-Jin Park Tae-San Kim Jae-Gyun Gwag Soon-Jae Kwon 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(7):1247-1254
The numbers of SSR markers and their utilization have not been determined and investigated as extensively in Fagopyrum species as compared to other crop species. The current report presents 136 new SSR markers in Fagopyrum esculentum ssp. esculentum and their application to related species in the genus Fagopyrum. Of the 136 SSRs, 10 polymorphic SSR markers were utilized in a genetic diversity analysis of a common buckwheat population
consisting of 41 accessions of diverse origin. The study showed observed (H
O) and expected (H
E) heterozygosities ranging from 0.071 to 0.924 (mean = 0.53) and from 0.073 to 0.902 (mean = 0.412), respectively. Forty-one
of the 136 SSRs amplified sequences in other Fagopyrum species, including the cymosum and urophyllum groups. The phylogenetic relationships revealed using the SSRs was consistent
with results obtained using other marker systems, with one exception. The sequence and diversity information obtained using
these new SSRs and their cross-transferability to related Fagopyrum species will increase our understanding of genetic structures and species relationships within the Fagopyrum genus. 相似文献
14.
Hui Feng Peng Wei Zhong-Yun Piao Zhi-Yong Liu Cheng-Yu Li Yu-Gang Wang Rui-Qin Ji Shu-Juan Ji Ting Zou Su-Ryun Choi Yong-Pyo Lim 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(2):333-339
The genic multiple-allele inherited male-sterile gene Ms in Chinese cabbage (Brassica rapa L.) was identified as a spontaneous mutation. Applying this gene to hybrid seed production, several B. rapa cultivars have been successfully bred in China. A BC1 population (244 plants) was constructed for mapping the Ms gene. Screening 268 simple sequence repeat (SSR) markers which cover the entire genome of Chinese cabbage was performed with
bulked segregant analysis (BSA). On the basis of linkage analysis, the Ms gene was located on linkage group R07. In addition, through the amplified fragment length polymorphism (AFLP) and the sequence-characterized
amplified region (SCAR) techniques combining BSA, two SCAR markers which were converted from corresponding AFLP markers flanked
the Ms gene. Finally, a genetic map of the Ms gene was constructed covering a total interval of 9.0 cM. Two SCAR markers, syau_scr01 and syau_scr04, flanked the Ms gene at distances of 0.8 and 2.5 cM, respectively. All the SSR markers (cnu_m273, cnu_m030, cnu_m295, and syau_m13) were
mapped on the same side of the gene as syau_scr04, the nearest one of which, syau_m13, was mapped at a distance of 3.3 cM.
These SSR and SCAR markers may be useful in marker-assisted selection and map-based cloning.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
15.
Molecular markers from a BAC contig spanning the Rdr1 locus: a tool for marker-assisted selection in roses 总被引:1,自引:0,他引:1
Anja Biber Helgard Kaufmann Marcus Linde Monika Spiller Diro Terefe Thomas Debener 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(4):765-773
We constructed a BAC contig of about 300 kb spanning the Rdr1 locus for black spot resistance in Rosa multiflora hybrids, using a new BIBAC library from DNA of this species. From this contig, we developed broadly applicable simple sequence
repeat (SSR) markers tightly linked to Rdr1, which are suitable for genetic analyses and marker-assisted selection in roses. As a source for the high molecular weight
DNA, we chose the homozygous resistant R.
multiflora hybrid 88/124-46. For the assembly of the BAC contig, we made use of molecular markers derived from a previously established
R. rugosa contig. In order to increase the resolution for fine mapping, the size of the population was increased to 974 plants. The
genomic region spanning Rdr1 is now genetically restricted to 0.2 cM, corresponding to a physical distance of about 300 kb. One single-stranded conformational
polymorphism (SSCP) and one SSR marker cosegregate with the Rdr1-mediated black spot resistance, while one SSR and several cleaved amplified polymorphic sequence or SSCP markers are very
tightly linked with one to three recombinants among the 974 plants. The benefits of the molecular markers developed from the
R. multiflora contig for the genetic analysis of roses and the integration of rose genetic maps are discussed. 相似文献
16.
Ya Zhang Li Xu Deng-Feng Zhang Jing-Rui Dai Shou-Cai Wang 《Molecular breeding : new strategies in plant improvement》2010,25(3):433-439
Southern corn rust, caused by Puccinia polysora Underw., has destructive potential on the susceptible host. In this study, the resistance inheritance was investigated in
an F
2 and its F
2:3 populations derived from a cross from two inbred lines W2D (resistant) and W222 (susceptible). The 3:1 ratio of resistant
to susceptible plants indicated that the resistance is controlled by one dominant gene (named as RppD). The gene RppD was located by means of the F
2 population. Total of 11 markers, including five SSR markers, five sequence-tagged site markers and one cleaved-amplified
polymorphic sequence (CAPS) marker, were identified to narrow the gene RppD down to a smaller interval. The closest markers flanking RppD were SSR marker umc1291 and CAPS marker CAPS858, with genetic distances of 2.9 and 0.8 cM, respectively. Moreover, RppD might be a novel Rpp resistance gene or haplotype differing from RppQ and RppP25 according to an allelism test among the three crosses W2D × Qi319, W2D × P25 and Qi319 × P25. As a result, RppD haplotype might be helpful to maize germplasm enhancement and disease-resistant breeding. 相似文献
17.
Yoko Yatabe Chie Tsutsumi Yumiko Hirayama Keigo Mori Noriaki Murakami Masahiro Kato 《Journal of plant research》2009,122(6):585-595
Rheophilous Osmunda lancea often hybridizes with a dryland ally, Osmunda japonica, to produce O. × intermedia, forming zonation in riverbanks and the adjacent dryland along flooding frequency clines. This study examined the genetic
structure of populations consisting of O. × intermedia and the two parental species by analyzing ten nuclear DNA markers [six cleaved amplified polymorphic sequence (CAPS) markers
and three simple sequence repeat (SSR) markers developed from an expressed sequence tag (EST) library, and the sequence of
the glyceraldehyde-3-phosphate dehydrogenase gene GapCp] and chloroplast DNA sequences. The results suggest that the nuclear genes of O. japonica and O. lancea are genetically differentiated despite shared polymorphism in their chloroplast DNA sequences. This discrepancy may be attributable
to natural selection and recent introgression, although it is not evident if introgression occurs between O. japonica and O. lancea in the examined populations. Our findings of putative F2 hybrids in O. × intermedia support its partial reproducibility, and also suggest that formation of later-generation hybrids generates morphological
variation in O. × intermedia. O. lancea plants collected from geographically distant localities were genetically very similar, and it is suggested that O. lancea originated monotopically. 相似文献
18.
Fifty-two genotypes of Eleusine coracana collected from Uttarakhand hills were subjected to simple sequence repeat (SSR), random amplified polymorphic DNA (RAPD)-PCR
and protein profiling analysis to investigate the variation in protein content. The main objective of the present study was
to detect variability among E. coracana and also assess the discriminating ability of these three molecular methods. A total of 21 RAPD and 24 SSR primers were assayed
for their specificity in detecting genetic variability in E. coracana, of which 20 RAPD and 21 SSR primers were highly reproducible and were found suitable for use in PCR analysis. Assessing genetic
diversity among E. coracana genotypes by RAPD-PCR using 20 polymorphic primers yielded 56 different RAPD markers which clustered the genotypes into different
groups on the basis of protein content. Similarly, SSR-PCR with 21 polymorphic primers clustered the genotypes into different
groups. On the other hand, biochemical typing of E. coracana using whole seed proteins generated profiles that showed no major difference indicating the technique to be not useful in
typing genotypes of this crop. However, a few of the genotypes showed the presence of a unique band of 32 kDa that needs to
be further investigated to understand the role of the protein from nutritional point of view, if any. In the present study,
significant negative correlation (r = −0.69*) was found between the protein and calcium content of finger millet genotypes. Sodium Dodecyl Sulphate Polyacrylamide
Gel Electrophoresis based seed storage proteins generated profiles showed no major differences in banding pattern among 52
finger millet genotypes while quantitative estimation of seed storage protein fractions using Lowry method revealed that glutelin
was highest followed by prolamin, globulin and albumin. 相似文献
19.
Liu XL Yang XF Wang CY Wang YJ Zhang H Ji WQ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(2):287-293
The English grain aphid, Sitobion avenae (Fabricius), is one of the most important insect pests causing substantial yield losses in wheat production in China and
other grain-growing areas in the world. The efficient utilization of wheat genes for resistance to English grain aphid (EGA)
provides an efficient, economic and environmentally sound approach to reduce the yield losses. In the present study, the wheat
line C273 (Triticum durum AABB, 2n = 4x = 28), is resistant to EGA in greenhouse and field tests. To identify the resistance gene, designated RA-1 temporarily, C273
was crossed with susceptible genotype Poland 305 (T. polonicum, AABB, 2n = 4x = 28). The F1, F2 and F2:3 lines were tested with EGA in the field and greenhouse. The results indicated that RA-1 is a single dominant gene, closely linked to the microsatellite markers (SSR) Xwmc179, Xwmc553 and Xwmc201 on chromosome 6AL at genetic distances of 3.47, 4.73 and 7.57 cM, respectively. The three SSR markers will be valuable in
marker-assisted selection for resistance to EGA as well as for cloning this gene in the future. 相似文献
20.
Identification and mapping of molecular markers linked to the tuberculate fruit gene in the cucumber (Cucumis sativus L.) 总被引:1,自引:0,他引:1
Weiwei Zhang Huanle He Yuan Guan Hui Du Lihua Yuan Zheng Li Danqing Yao Junsong Pan Run Cai 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(3):645-654
Warty fruit is one of the highly valuable external quality traits related to the market values of cucumber. Genetic analysis
has shown that a single dominant gene, Tu (Tuberculate fruit), determines the warty fruit trait in the cucumber plant. An F2 population (247 individuals) from the cross of S06 × S52 was used for the mapping of the Tu/tu locus. By combining bulked segregant analysis with the sequence-related amplified polymorphism (SRAP) and simple sequence
repeat (SSR) markers, 15 markers (9 SRAPs and 6 SSRs) linked to the Tu/tu locus were identified. Of nine SRAP markers, three closely linked to the Tu/tu locus were successfully converted into sequence characterized amplified region (SCAR) markers. The Tu/tu locus was mapped between the co-dominant SSR marker SSR16203 and the SCAR marker C_SC933, at a genetic distance of 1.4 and
5.9 cM, respectively. Then the linked SSR markers in the study were used as anchor loci to locate the Tu/tu locus on cucumber chromosome 5. Moreover, the validity analysis of the C_SC69 and C_SC24 markers was performed with 62 cucumber
lines of diverse origins, showing that the two SCAR markers can be used for marker-assisted selection (MAS) of the warty fruit
trait in cucumber breeding. The information provided in this study will facilitate the map-based cloning of the Tu/tu gene. 相似文献