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1.
《The International journal of biochemistry》1984,16(2):177-182
- 1.1. Protein synthesis by GTP -supplemented yeast mitochondria is stimulated by a fraction of molecular weight less than 2,000 isolated from yeast high-speed supernatant (S-150).
- 2.2. The low molecular weight fraction works independently of the respiratory chain as the stimulation effect is not cyanide-sensitive.
- 3.3. Stimulation of mitochondrial protein synthesis by cytoplasmic factors is dependent upon the method of mitochondrial isolation.
- 4.4. The low molecular weight stimulatory factor(s) are not reduced folate derivatives which supply formyl groups required for initiation of mitochondrial protein synthesis.
2.
《The International journal of biochemistry》1983,15(11):1305-1312
- 1.1. A basic fraction from brain cytosol was found to contain two or more tubulin-binding proteins, able to induce aggregation of tubulin accompanied by hydrolysis of GTP.
- 2.2. In some respects tubulin aggregation by the brain proteins was similar to its aggregation by polylysine.
- 3.3. The burst of GTP hydrolysis accompanying tubulin aggregation by polylysine had the following characteristics: enhanced by salt and abolished by low temperature; not stoichiometric with the amount of tubulin precipitated and actually maximal at relatively low polylysine concentration; uncoupled temporally from aggregation, which occurred over a much shorter interval.
3.
《The International journal of biochemistry》1983,15(4):495-500
- 1.1. Several glutamate analogues substituted at the β- or γ-carbon atoms have been tested as substrates for glutamate dehydrogenase.
- 2.2. The two γ-methyl derivatives and DL-β-methylglutamate give the same pH optimum (8.7) as l-glutamate, but show inhibition by ADP and activation by GTP at pH 8, unlike glutamate and like the monocarboxylic substrate l-norvaline, which gives a pH optimum of 10.
- 3.3. l-γ-methyleneglutamate, the poorest substrate tested (0.28% of rate with glutamate) gives a high pH optimum (10), like norvaline, but shows marked activation by both ADP (13-fold) and GTP (27-fold).
- 4.4. Despite the correct dicarboxylate spacing, all the analogues were much poorer substrates than l-norvaline.
4.
《Biometric Technology Today》2003,11(10):5
Including information on:
- ScanSoft
- SpeechWorks International
- Viisage Technology
- Firstec
- BIO-key International
- HP
- ZN Vision Technologies
- Unisys
- US Government’s
- Communication Intelligence Corporation
- Infinity Technologies
5.
《Biometric Technology Today》2003,11(11):6
- Daon
- Musicrypt
- EMI Music Canada
- Digital Broadband Networks
- FaceKey Corporation
- Eystar Media Inc (EMI)
- Temasya Wira
- Animated Electronic Industries
- BIO-key International
- Entryport Corporation
6.
《Comparative biochemistry and physiology. A, Comparative physiology》1992,101(1):157-162
- 1.1. Oxygen carrying capacity and parameters of erythrocyte-oxygen binding are similar for a range of elasmobranchs with markedly different swimming behaviour.
- 2.2. Erythrocyte nucleotide components in sharks include the allosteric hemoglobin modifiers GTP and ATP in similar ratios, and the total pool appears independent of locomotory activity. A rhinobatoid ray had no detectable erythrocyte trinucleotides, but had an appreciable pool of AMP together with IMP.
- 3.3. There was no evidence for either urea or NaCl modulation of hemoglobin function in erythrocytes from a carcharhinid shark.
- 4.4. These observations lead to the conclusion that parameters of the oxygen transport system in elasmobranchs are highly conserved.
7.
《Biometric Technology Today》2003,11(10):3
Including information on:
- Martin State Airport
- Bioscrypt
- Saflink
- Office of the Secretary of Defense
- Department of Defense
- Boeing Corporation
- Bell ID, Gemplus
- Siemens
- Foreign Ministry
8.
《Biometric Technology Today》2003,11(9):5
- Bioscrypt
- Saflink
- Dell
- Fujitsu Microelectronics America
- Identix
- Viisage
- Acsys Biometrics
- US Government
9.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1990,95(3):435-440
- 1.1. Hemoglobin, hematological parameters, intraerythrocytic phosphates and whole blood Bohr effect of Pterygoplichthys multiradiatus, from the Amazon river, were studied in three different conditions: in their natural environment, acclimated to normoxia and acclimated hypoxia conditions.
- 2.2. Nine anodal hemoglobin fractions were detected on starch gel electrophoresis. No qualitative differences in the Hb electrophoretic patterns were detected in the three studied groups.
- 3.3. Hematocrit, hemoglobin concentration, MCV, MCHC and MCH were different among studied conditions.
- 4.4. GTP was almost absent in the blood of animals in natural conditions and acclimated to hypoxia, but was present at a concentration similar to ATP in normoxic acclimated animals.
- 5.5. There is a tendency for higher Hb-O2 affinity for hypoxic acclimated/acclimatized animals.
10.
《The International journal of biochemistry》1993,25(1):79-86
- 1.1. Four GTP-binding proteins (23–27 kDa) were identified in membranes from PC12 cells by [α32P]GTP binding to nitrocellulose blots of SDS-polyacrylamide gels.
- 2.2. The GTP-binding proteins remained associated with membranes during stimulation of intact cells by K+-depolarization or even after addition of C2+to digitonin-permeabilized cells.
- 3.3. By two-dimensional gel electrophoresis, six GTP-binding proteins were resolved and based on their mobility, their phosphorylation state appeared independent of Ca2+.
- 4.4. Fractionation of PC12 membranes showed that these GTP-binding proteins were broadly distributed in post-nuclear membranes with the plasma membranes containing the highest specific GTP-binding activity.
- 5.5. Membrane fractions from bovine adrenal medulla contain similar GTP-binding proteins with GTP-binding intensity also being highest in the plasma membrane.
- 6.6. The GTP-binding proteins could be concentrated in the detergent-rich fraction upon Triton X-114 phase separation.
11.
《Ethology and sociobiology》1988,9(5):319-324
This paper comments on: Low, B. S., Alexander, R. D., and Noonan, K.M. Human hips, breast, and buttocks: Is fat deceptive? Ethology and Sociobiology 8: 249-247, 1987. In it I argue that:
- 1.1. Sexual selection has probably not been the most important selection pressure on
- 2.female human body shape.
- 3.2. Male humans in different cultures find different aspects of the female body attractive
- 4.and therefore are unlikely to have exerted consistent directional sexual selection on
- 5.the female body.
- 6.3. Breast size is not correlated with lactation success.
- 7.4. Visible hip width is not correlated with parturition success.
- 8.5. Women would lower their fitness if they tried to deceive men about their internal
- 9.pelvic dimensions.
- 10.6. There are many alternative hypothesis to explain the existence of fat onwomen's
- 11.breast, hips, and buttocks.
12.
《The International journal of biochemistry》1993,25(6):885-890
- 1.1. Among the digestive enzymes synthesized by pancreas, lipase is the principle lipolytic enzyme which hydrolyses dietary glycerides.
- 2.2. For its action it requires a coenzyme, colipase.
- 3.3. The molecular mechanisms of the interaction of these two are not fully understood.
- 4.4. Further, molecular events that regulate and influence lipid absorption are ill denned.
- 5.5. The rabbit is the conventional animal model for the study of lipid absorption. We have undertaken the molecular cloning, and characterization of rabbit pancreatic colipase, the coenzyme for pancreatic lipase.
- 6.6. Colipase has been cloned from a gt 11 library of an adult rabbit pancreatic cDNA by probing with an oligonucleotide derived from human colipase sequence.
- 7.7. The total reading frame consists of 321 nucleotides coding for 90 amino acids of the functional protein and 17 nucleotides of the leader peptide.
- 8.8. Northern blot analysis revealed a distinct band around 0.5kb. Comparison with other species revealed an over all homology of 75% at the nucleotide level.
- 9.9. At the amino acid level highest conservation is observed at the lipase-binding region (AA 53–73).
- 10.10. Rabbit enzyme also retained the N-terminal pentapeptide of it preform.
- 11.11. The regions of homology and conservation may aid to define the sites of interaction of colipase with lipase.
13.
《The International journal of biochemistry》1985,17(5):589-595
- 1.1. A quick and simple procedure is described for purifying kallikrein from human whole saliva. The enzyme has been purified about 2700-fold with a yield of approx. 30%.
- 2.2. The procedure is based on the immediate fractionation of saliva by ion exchange chromatography. This is followed by a combination of affinity and high performance liquid chromatography.
- 3.3. The results indicate that another protein component binds to the enzyme at pH 8.0.
- 4.4. The homogeneity of the enzyme has been demonstrated by gel electrophoresis in the absence as well as in the presence of sodium dodecylsulfate.
- 5.5. A mol. wt of 40,100±1800 has been calculated from gel electrophores is experiments.
- 6.6. Sedimentation equilibrium in an analytical ultracentrifuge gave a mol. wt of 39,700.
- 7.7. The amino acid composition has been determined and it confirms that the enzyme has a low isoelectric point.
- 8.8. The presence of tryptophan has been demonstrated by absorption and fluorescence spectroscopy.
14.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1986,83(2):347-353
- 1.1. Two vitellogenins and chromoprotein 2 are selectively accumulated by the oocyte and cannot be detected either in follicle cells or in the germarium.
- 2.2. At the start of their accumulation in terminal oocytes they are asymmetrically distributed.
- 3.3. Endocytosis of vitellogenin 1 starts somewhat later than the uptake of vitellogenin 2 and chromoprotein 2.
- 4.4. In follicle cells of young follicles, a protein (DLP), immunologically related to diapause protein 1, is highly concentrated.
- 5.5. During vitellogenesis DLP is sequestered by the oocytes.
- 6.6. The protein rich globules in terminal oocytes contain the vitellins as well as chromoprotein 2 and DLP.
15.
《Comparative biochemistry and physiology. A, Comparative physiology》1986,83(3):597-601
- 1.1. Haemolymph volume decreases during the initial 16 hr post-ecdysial period, increases after water ingestion and subsequently drops until the inter-ecdysial level is reached.
- 2.2. Total body water follows a similar pattern, but the changes are not as pronounced.
- 3.3. Tissue water is inversely proportional to the total body water.
- 4.4. Soluble cuticle protein declines throughout the initial 16 hr period while both β-glucosidase and alkaline phosphatase activity is lost within 6 hr after ecdysis.
- 5.5. Dehydration of the cuticle also occurs during the immediate 6 hr post-ecdysial period.
- 6.6. These data suggest that the formation of the protein-insoluble matrix is linked with water loss.
- 7.7. Water removal may decrease the distance between molecules allowing specific reactions to take place.
16.
《The International journal of biochemistry》1984,16(12):1237-1243
- 1.1. Human placental alkaline phosphatase was inactivated with tetranitromethane in a biphasic process.
- 2.2. Spectral and amino acid analysis demonstrated that the inactivation was due to the conversion of tyrosine residues to 3-nitrotyrosine.
- 3.3. The inactivation process showed saturation kinetics.
- 4.4. Protection of the enzyme against tetranitromethane inactivation was afforded by inorganic phosphate.
- 5.5. The binding affinity between the modified enzyme and inorganic phosphate was decreased.
- 6.6. Our results suggest the involvement of tyrosyl residues in the locus of phosphoryl site of the phosphorylated enzyme forms.
17.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1992,101(4):963-967
- 1.1. Vitellogenin (VG) was isolated and purified from the hemolymph of female American cockroaches.
- 2.2. The purification method used in this study comprises two steps: the first step is based on the method originally developed for purifying lipophorin from hemolymph, and the second step is the separation of VG from lipophorin by a KBr density gradient ultracentrifugation.
- 3.3. The purified VG was characterized according to molecular weight, substructure, shape and size, and lipid composition.
- 4.4. The VG molecule is almost globular in shape with the diameter of about 15.5 nm and is indistinguishable from lipophorin in shape and size.
- 5.5. The native molecular weight determined by light scattering method was 560 kDa.
- 6.6. The VG consists of four subunits with molecular weights of approximately 102, 81, 49 and 40 kDa, respectively.
- 7.7. VG is a lipoprotein and comprises 92% protein and 8% lipid.
- 8.8. Major lipid components were found to be diacylglycerol (25%) and phospholipids (71%).
18.
《The International journal of biochemistry》1993,25(2):157-161
- 1.1. To understand the physiological roles of the 90-kDa stress protein (HSP90), we investigated the heparin- and antibody-binding domains of the protein.
- 2.2. For heparin-binding sites, HSP90 was digested completely with trypsin, and the digests were applied to a heparin-Sepharose column and eluted with 1.0 M NaCl, followed by 8.0 M urea.
- 3.3. Each elutant was purified by a reverse-phase C18 column.
- 4.4. Two peptides from the NaCl-eluted fraction and no peptide from the urea-eluted fraction were purified.
- 5.5. The purified peptides were sequenced by an automated peptide sequencer.
- 6.6. One of the heparin-binding sites was present between Leu-362 and Arg-365; another was present between Leu-645 and Lys-648.
- 7.7. These two peptides were basic and considerably hydrophilic.
- 8.8. For antibody-binding sites, HSP90 was mildly digested with trypsin, electrophoresed on SDS-polyacrylamide gels and transferred to PVDF membranes.
- 9.9. The four bound of the trypsin fragments could be sequenced with a peptide sequencer.
- 10.10. There was only one antibody-binding peptide, 38 kDa, starting from Pro-2. The others showed no cross-reactivity with the antibody and started from Leu-283.
- 11.11. Therefore, the epitopes of HSP90 are present between Pro-2 and Leu-282.
- 12.12. The heparin-binding sites are present from the middle region of the HSP90 molecule, and the antigen sites are at the N-terminal domain.
19.
《The International journal of biochemistry》1991,23(3):293-299
- 1.1. In a continuing investigation of phycocyanin-membrane surface interaction, fluorescence quenching experiments were performed with a mixture of two populations of fluorescence probe-encapsulated phospholipid bilayer vesicles in the presence and absence of phycocyanin.
- 2.2. These membrane vesicles were prepared with 1,2-dimyristoyl phosphatidylcholine (DMPC), cholesterol and a probe molecule.
- 3.3. A fluorophore was encapsulated in one population of membrane vesicles, while a quencher was encapsulated in another population of membrane vesicles.
- 4.4. The result was compared with those of experiments in the presence of other biomolecules, including albumin, cytochrome c, hemoglobin, myoglobin or RNA.
- 5.5. Interestingly, a one-third reduction of the fluorescence intensity was observed in the mixture of these two populations of membrane vesicles in phycocyanin's presence.
- 6.6. In contrast, the other biomolecules caused no significant reduction in the fluorescence intensity.
- 7.7. These findings were evidence of a phycocyanin-induced membrane perturbation.
- 8.8. This was further demonstrated by a phycocyanin-induced change in the thermotropic behavior of DMPC vesicles, as measured by differential scanning microcalorimetry.
- 9.9. Such a unique property of phycocyanin is believed to be associated with its known membrane surface-interacting character.
- 10.10. A possible phycocyanin-modulated membrane-membrane interaction was discussed.
20.
《Comparative biochemistry and physiology. A, Comparative physiology》1987,86(2):239-241
- 1.1. A variety of haematological parameters were determined in adult Dasyurus viverrinus.
- 2.2. Haemoglobin and red cell counts were high with a very low mean cell volume.
- 3.3. Basophils are absent but the eosinophils contain small numbers of basophilic granules which may indicate a dual role for this cell.
- 4.4. “Ring Form” leucocytes are present.
- 5.5. Three types of red cell picture could be identified, some animals showing large numbers of spherocytes, spicule cells, and inclusion bodies.
- 6.6. These cells resemble those found in some inherited human haemolytic anaemias but there was no evidence of haemolysis in the animals.
- 7.7. An alkali resistant haemoglobin component is present.