Willingness to Pay for Eco‐Certified Refurbished Products: The Effects of Environmental Attitudes and Knowledge

Refurbishing products, which are increasingly sold in business‐to‐consumer markets, is a key strategy to reduce waste. Nevertheless, research finds that consumers’ willingness to pay (WTP) for refurbished products is low. Strategies for a higher WTP are needed in order to grow consumer markets for refurbished products. Eco‐certification of refurbished products may be a key strategy here. Drawing on the consumer WTP literature concerning “green” products, we investigate the impact of independent eco‐certificates. Our analysis is based on a survey of 231 potential customers. The results suggest that, across various product categories, the WTP for products with refurbished components is significantly lower. Adding an eco‐certificate tends to return the WTP toward the virgin product level. We show that consumers with proenvironmental attitudes particularly exhibit green buying behavior. Our findings indicate that eco‐certification is often worthwhile because it enhances the business rationale for producing products with refurbished components.     

Case study and application of process analytical technology (PAT) towards bioprocessing: Use of tryptophan fluorescence as at‐line tool for making pooling decisions for process chromatography

Process analytical technology (PAT) has been gaining momentum in the biopharmaceutical community due to the potential for continuous real time quality assurance resulting in improved operational control and compliance. Two imperatives for implementing any PAT tool are that “variability is managed by the process” and “product quality attributes can be accurately and reliably predicted over the design space established for materials used, process parameters, manufacturing, environmental, and other conditions.” Recently, we have been examining the feasibility of applying different analytical tools to bioprocessing unit operations. We have previously demonstarted that commercially available online‐high performance liquid chromatography and ultra performance liquid chromatography systems can be used for analysis that can facilitate real‐time decisions for column pooling based on product quality attributes (Rathore et al., 2008 a,b). In this article, we review an at‐line tool that can be used for pooling of process chromatography columns. We have demonstrated that our tryptophan fluorescence method offers a feasible approach and meets the requirements of a PAT application. It is significantly faster than the alternative of fractionation, offline analysis followed by pooling. Although the method as presented here is not an online method, this technique may offer better resolution for certain applications and may be a more optimal approach as it is very conducive to implementation in a manufacturing environment. This technique is also amenable to be used as an online tool via front face fluorescence measurements done concurrently with product concentration determination by UV. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009     

Process analytical technology (PAT) for biopharmaceutical products: Part II. Concepts and applications

Implementing real‐time product quality control meets one or both of the key goals outlined in FDA's PAT guidance: “variability is managed by the process” and “product quality attributes can be accurately and reliably predicted over the design space established for materials used, process parameters, manufacturing, environmental, and other conditions.” The first part of the paper presented an overview of PAT concepts and applications in the areas of upstream and downstream processing. In this second part, we present principles and case studies to illustrate implementation of PAT for drug product manufacturing, rapid microbiology, and chemometrics. We further present our thoughts on how PAT will be applied to biotech processes going forward. The role of PAT as an enabling component of the Quality by Design framework is highlighted. Integration of PAT with the principles stated in the ICH Q8, Q9, and Q10 guidance documents is also discussed. Biotechnol. Bioeng. 2010; 105: 285–295. Published 2009 Wiley Periodicals, Inc.     

利益相关者视角下的濒危物种价值评估与生态补偿——以翅果油树为例

濒危物种价值评估可为生物多样性保护提供重要的政策支撑。本文以国家二级重点保护植物翅果油树为例,从利益相关者视角作为切入点,基于选择实验法评价其偏好和支付意愿(WTP),进而探讨生态补偿机制。基于管理部门、企业和农户三方利益相关者的利益诉求,分析确定了5个翅果油树的物种属性,即种植面积、产品分类、树苗品种、保护投入和农户收益。运用随机参数Logit模型分析了利益相关者对不同物种属性变量的偏好和支付意愿。结果表明: 受访者对“保护增加投入”偏好最强和支付意愿最高,每户为331.00 yuan·a^-1;对产品开发的“高低端产品同时开发”和“高端产品开发为主”变量偏好次之,WTP分别为每户242.71和227.57 yuan·a^-1;对“农户收益”、“保护投入不变”、“树苗品种”和“种植面积”由强到弱依次表现出一定的偏好及支付意愿;而对“保护不投入”和“低端产品开发为主”偏好最弱且WTP为负值。通过直接和间接补偿方式对补偿客体(翅果油树及其生境)实施生态补偿,得到的补偿剩余价值为每户每年285.62元。     

Bioprocessing automation in cell therapy manufacturing: Outcomes of special interest group automation workshop

Phacilitate held a Special Interest Group workshop event in Edinburgh, UK, in May 2017. The event brought together leading stakeholders in the cell therapy bioprocessing field to identify present and future challenges and propose potential solutions to automation in cell therapy bioprocessing. Here, we review and summarize discussions from the event. Deep biological understanding of a product, its mechanism of action and indication pathogenesis underpin many factors relating to bioprocessing and automation. To fully exploit the opportunities of bioprocess automation, therapeutics developers must closely consider whether an automation strategy is applicable, how to design an ‘automatable’ bioprocess and how to implement process modifications with minimal disruption. Major decisions around bioprocess automation strategy should involve all relevant stakeholders; communication between technical and business strategy decision-makers is of particular importance. Developers should leverage automation to implement in-process testing, in turn applicable to process optimization, quality assurance (QA)/ quality control (QC), batch failure control, adaptive manufacturing and regulatory demands, but a lack of precedent and technical opportunities can complicate such efforts. Sparse standardization across product characterization, hardware components and software platforms is perceived to complicate efforts to implement automation. The use of advanced algorithmic approaches such as machine learning may have application to bioprocess and supply chain optimization. Automation can substantially de-risk the wider supply chain, including tracking and traceability, cryopreservation and thawing and logistics. The regulatory implications of automation are currently unclear because few hardware options exist and novel solutions require case-by-case validation, but automation can present attractive regulatory incentives.     

What Affects the Secondhand Value of Smartphones: Evidence from eBay

Reuse via secondhand markets can extend the use phase of products, thereby reducing environmental impacts. Analyzing 500,000 listings of used Apple and Samsung smartphones sold in 2015 and 2016 via eBay, we examine which product properties affect how long smartphones retain market value and facilitate market‐based reuse. Our results suggest that although repairability and large memory size are typically thought to be “life extending,” in practice they have limited impact on the current economic life span of smartphones and their market‐based reuse. In contrast, we show that brand, an intangible product property, can extend smartphones’ economic life span by 12.5 months. Because longer economic life spans imply extended use phases and longer life spans overall, these results illustrate the potential of harnessing the intangible properties of products to promote sustainable consumption.     

Large scale demonstration of a process analytical technology application in bioprocessing: Use of on‐line high performance liquid chromatography for making real time pooling decisions for process chromatography

Process Analytical Technology (PAT) has been gaining a lot of momentum in the biopharmaceutical community because of the potential for continuous real time quality assurance resulting in improved operational control and compliance. In previous publications, we have demonstrated feasibility of applications involving use of high performance liquid chromatography (HPLC) and ultra performance liquid chromatography (UPLC) for real‐time pooling of process chromatography column. In this article we follow a similar approach to perform lab studies and create a model for a chromatography step of a different modality (hydrophobic interaction chromatography). It is seen that the predictions of the model compare well to actual experimental data, demonstrating the usefulness of the approach across the different modes of chromatography. Also, use of online HPLC when the step is scaled up to pilot scale (a 2294 fold scale‐up from a 3.4 mL column in the lab to a 7.8 L column in the pilot plant) and eventually to manufacturing scale (a 45930 fold scale‐up from a 3.4 mL column in the lab to a 158 L column in the manufacturing plant) is examined. Overall, the results confirm that for the application under consideration, online‐HPLC offers a feasible approach for analysis that can facilitate real‐time decisions for column pooling based on product quality attributes. The observations demonstrate that the proposed analytical scheme allows us to meet two of the key goals that have been outlined for PAT, i.e., “variability is managed by the process” and “product quality attributes can be accurately and reliably predicted over the design space established for materials used, process parameters, manufacturing, environmental, and other conditions”. The application presented here can be extended to other modes of process chromatography and/or HPLC analysis. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010     

The business perspective in ecological restoration: issues and challenges

Much of the practice of restoration is conducted by businesses—contractors, consultants, designers, engineers. Restoration businesses interact with a variety of stakeholders to complete projects on time and on budget, and to achieve ecological and business objectives. Our research explores the business perspective in restoration; it is based on data collected from businesses (contractors, consultants, design engineers), agencies, and nongovernmental organizations involved in a Superfund cleanup project in Montana, one of the largest river restoration efforts ever. Our findings highlight several areas restoration businesses must navigate. First, restoration businesses must juggle potentially competing goals, maintaining ecological integrity while achieving profitability objectives. Second, these businesses must manage the risk that arises from variability in the natural environment as well as individuals' risk tolerances. Third, they must navigate the disconnect between “science” and “practice,” including how to best monitor restoration projects. Fourth, they must make decisions about new techniques and innovations. Fifth, on‐the‐ground implementation must acknowledge that personnels' motives and expertise might conflict with original plans. We discuss these findings in relation to relevant scholarly research, offering implications for theory and practice. For example, the business of ecological restoration requires learning over time to be profitable while achieving the desired ecological and social outcomes; restoration businesses leverage monitoring in pursuit of adaptive management and engage “frontline personnel” as important voices in the restoration process. Understanding the business of restoration adds an important perspective in the complex dynamics of social‐ecological systems.     

Chemical Management Services in Sweden and Europe: Lessons for the Future

The number and diversity of chemicals produced and used in society today are growing in conjunction with the both evident and uncertain environmental impacts associated with the life cycles of these chemicals. Chemical management services (CMS) is a business strategy based on a strategic, long-term contract, according to which the supplier of chemical management services accepts the responsibility for managing chemicals and strives to reduce the associated costs and risks. This strategy also has the potential for reducing the environmental impacts of chemicals. This article provides an overview of the existing advantages and barriers for CMS providers and customers in the European context, identifies conflicts of interest between them, and highlights important lessons regarding the role of CMS in shaping these markets. It reports on findings from interviews with European chemical producers and other stakeholders of chemical management services and is directed toward industry professionals interested in chemical management services. It concludes that economic and environmental advantages of CMS are not automatically guaranteed and lists factors that are critical for developing a win-win CMS for both providers and customers. Finally, ways of fostering CMS dissemination in Europe are suggested.     

Potential for industrial ecology to support healthcare sustainability: Scoping review of a fragmented literature and conceptual framework for future research

Healthcare is a critical service sector with a sizable environmental footprint from both direct activities and the indirect emissions of related products and infrastructure. As in all other sectors, the “inside‐out” environmental impacts of healthcare (e.g., from greenhouse gas emissions, smog‐forming emissions, and acidifying emissions) are harmful to public health. The environmental footprint of healthcare is subject to upward pressure from several factors, including the expansion of healthcare services in developing economies, global population growth, and aging demographics. These factors are compounded by the deployment of increasingly sophisticated medical procedures, equipment, and technologies that are energy‐ and resource‐intensive. From an “outside‐in” perspective, on the other hand, healthcare systems are increasingly susceptible to the effects of climate change, limited resource access, and other external influences. We conducted a comprehensive scoping review of the existing literature on environmental issues and other sustainability aspects in healthcare, based on a representative sample from over 1,700 articles published between 1987 and 2017. To guide our review of this fragmented literature, and to build a conceptual foundation for future research, we developed an industrial ecology framework for healthcare sustainability. Our framework conceptualizes the healthcare sector as comprising “foreground systems” of healthcare service delivery that are dependent on “background product systems.” By mapping the existing literature onto our framework, we highlight largely untapped opportunities for the industrial ecology community to use “top‐down” and “bottom‐up” approaches to build an evidence base for healthcare sustainability.     

An emergy analysis-based methodology for eco-efficiency evaluation of building manufacturing

Building manufacturing creates product value like building space, and induces severe environmental influence at the same time. The idea of eco-efficiency (EE) can link a product or service value and the corresponding environmental influence together, but there has been little research that implements such idea on building manufacturing, such as the green building rating system of the comprehensive assessment system for building environmental efficiency (CASBEE) in Japan and the EcoEffect in Sweden. This paper proposes an emergy (spelled with an “m”) analysis-based methodology for EE evaluation of building manufacturing, where the building space (in cubic size) and the emergy amount of building manufacturing are selected as the indicators of product value and the environmental influence, respectively. Six residential buildings that include multi-storeys, moderate high-rises and high rises are selected averagely from two Chinese famous cities, namely Beijing and Shanghai. The evaluation results can provide directions to assess environmental influence and thus to heighten the EE of building manufacturing. The EE evaluation result can even be used as the base of a new green building (or eco-building) rating system.     

Raw material criticality assessment as a complement to environmental life cycle assessment: Examining methods for product‐level supply risk assessment

The diversity of raw materials used in modern products, compounded by the risk of supply disruptions—due to uneven geological distribution of resources, along with socioeconomic factors like production concentration and political (in)stability of raw material producing countries—has drawn attention to the subject of raw material “criticality.” In this article, we review the state of the art regarding the integration of criticality assessment, herein termed “product‐level supply risk assessment,” as a complement to environmental life cycle assessment. We describe and compare three methods explicitly developed for this purpose—Geopolitical Supply Risk (GeoPolRisk), Economic Scarcity Potential (ESP), and the Integrated Method to Assess Resource Efficiency (ESSENZ)—based on a set of criteria including considerations of data sources, uncertainties, and other contentious methodological aspects. We test the methods on a case study of a European‐manufactured electric vehicle, and conclude with guidance for appropriate application and interpretation, along with opportunities for further methodological development. Although the GeoPolRisk, ESP, and ESSENZ methods have several limitations, they can be useful for preliminary assessments of the potential impacts of raw material supply risks on a product system (i.e., “outside‐in” impacts) alongside the impacts of a product system on the environment (i.e., “inside‐out” impacts). Care is needed to not overlook critical raw materials used in small amounts but nonetheless important to product functionality. Further methodological development could address regional and firm‐level supply risks, multiple supply‐chain stages, and material recycling, while improving coverage of supply risk characterization factors.     

Environmental DNA metabarcoding: Transforming how we survey animal and plant communities

The genomic revolution has fundamentally changed how we survey biodiversity on earth. High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA from diverse kinds of environmental samples (termed “environmental DNA” or “eDNA”). Coupling HTS with our ability to associate sequences from eDNA with a taxonomic name is called “eDNA metabarcoding” and offers a powerful molecular tool capable of noninvasively surveying species richness from many ecosystems. Here, we review the use of eDNA metabarcoding for surveying animal and plant richness, and the challenges in using eDNA approaches to estimate relative abundance. We highlight eDNA applications in freshwater, marine and terrestrial environments, and in this broad context, we distill what is known about the ability of different eDNA sample types to approximate richness in space and across time. We provide guiding questions for study design and discuss the eDNA metabarcoding workflow with a focus on primers and library preparation methods. We additionally discuss important criteria for consideration of bioinformatic filtering of data sets, with recommendations for increasing transparency. Finally, looking to the future, we discuss emerging applications of eDNA metabarcoding in ecology, conservation, invasion biology, biomonitoring, and how eDNA metabarcoding can empower citizen science and biodiversity education.     

Toward community predictions: Multi‐scale modelling of mountain breeding birds' habitat suitability,landscape preferences,and environmental drivers

Across a large mountain area of the western Swiss Alps, we used occurrence data (presence‐only points) of bird species to find suitable modelling solutions and build reliable distribution maps to deal with biodiversity and conservation necessities of bird species at finer scales. We have performed a multi‐scale method of modelling, which uses distance, climatic, and focal variables at different scales (neighboring window sizes), to estimate the efficient scale of each environmental predictor and enhance our knowledge on how birds interact with their complex environment. To identify the best radius for each focal variable and the most efficient impact scale of each predictor, we have fitted univariate models per species. In the last step, the final set of variables were subsequently employed to build ensemble of small models (ESMs) at a fine spatial resolution of 100 m and generate species distribution maps as tools of conservation. We could build useful habitat suitability models for the three groups of species in the national red list. Our results indicate that, in general, the most important variables were in the group of bioclimatic variables including “Bio11” (Mean Temperature of Coldest Quarter), and “Bio 4” (Temperature Seasonality), then in the focal variables including “Forest”, “Orchard”, and “Agriculture area” as potential foraging, feeding and nesting sites. Our distribution maps are useful for identifying the most threatened species and their habitat and also for improving conservation effort to locate bird hotspots. It is a powerful strategy to improve the ecological understanding of the distribution of bird species in a dynamic heterogeneous environment.     

生态系统价值在政策制度设计中的应用——以福州市为例

在探索生态系统价值可复制、可推广、可示范的核算模式的基础上,如何合理应用生态系统价值核算结果、形成生态系统价值政策制度应用体系、促进生态系统服务价值由“核算值”向“政策点”转化是目前亟待解决的问题。本研究以福州市经济生态生产总值(GEEP)和生态系统生产总值(GEP)核算为切入点,对GEEP进行指标筛选,并计算绿金指数和单位面积价值量,分析其在政策制度设计中的应用模式。结果表明: 污染物吸收量、物种保育更新能值、湿地重度威胁面积占比、空气负氧离子释放量、耕地占用率、海洋围填海面积占比、固碳量、释氧量、草地覆盖变化率、海岸防护面积、固体废弃物环境退化成本是显著影响福州市GEEP核算结果的指标;福州市2015和2018年绿金指数分别为1.59和1.23,均高于全国水平;福州市各生态系统单位面积价值量大小依次为湿地生态系统＞森林生态系统＞农田生态系统＞草地生态系统＞海洋生态系统。基于福州市GEEP影响因素和绿金指数、单位面积价值量,本研究构建了福州市生态系统价值核算结果“进决策、进规划、进考核、进监测”的管理制度和模式,为我国形成助推生态文明建设的长效机理机制提供了理论依据。     

Does It Really Pay to Be Green? An Empirical Study of Firm Environmental and Financial Performance: An Empirical Study of Firm Environmental and Financial Performance

Previous empirical work suggests that firms with high environmental performance tend to be profitable, but questions persist about the nature of the relationship. Does stronger environmental performance really lead to better financial performance, or is the observed relationship the outcome of some other underlying firm attribute? Does it pay to have cleanrunning facilities or to have facilities in relatively clean industries? To explore these questions, we analyze 652 U.S. manufacturing firms over the time period 1987–1996. Although we find evidence of an association between lower pollution and higher financial valuation, we find that a firm's fixed characteristics and strategic position might cause this association. Our findings suggest that “When does it pay to be green?” may be a more important question than “Does it pay to be green?”     

31 Effects of light and ammonium on growth,ammonium uptake and pigmentation of four porphyra (bangiales,rhodophyta) species native to the northwestern atlantic

“Phytopia: Discovery of the Marine Ecosystem” is an educational CD‐ROM that features a wealth of new images, animations, microscope‐ and satellite‐derived data sets, and multimedia discovery tools. It has received support from NASA and the National Science Foundation and can ordered on‐line through http://www.bigelow.org/phytopia . A pre‐release version of the “Phytopia” was tested by students, educators, and scientists at 34 institutions in Britain, Germany, Russia, and throughout the U.S. This product is designed for use in undergraduate classes; however, our testing has shown that it provides an exciting and interactive learning experience appropriate for Grades 7 and higher. The virtual microscope tool allows the user to view prepared assemblages of plankton at various magnifications, under different epifluorescence conditions, or by scanning electron microscopy. For several species, the user can view movies showing the organism's motility. Some species are featured as three‐dimensional models that can be viewed from any perspective. “Phytopia” also helps users connect ocean primary productivity patterns with environmental factors in several geographic areas. Innovative tools allow investigation of co‐registered temperature, wind, current, nutrient, and ocean color data. This tool is aligned with research efforts to better understand plankton ecology using remotely sensed data.     

In vivo genetic manipulation of the rat trophoblast cell lineage using lentiviral vector delivery

In this report, we have adapted a lentiviral gene delivery technique for genetic modification of the rat trophoblast cell lineage. Blastocysts were incubated with lentiviral particles and transferred into the uteri of pseudopregnant female rats, harvested at various times during gestation, and then analyzed. Two test systems were evaluated: (1) delivery of an enhanced green fluorescent protein (EGFP) gene under the control of constitutive promoters to rat blastocysts; (2) delivery of EGFP short hairpin RNA (shRNA) to rat blastocysts constitutively expressing EGFP. Lentiviral packaged gene constructs were efficiently and specifically delivered to all trophoblast cell lineages. Additionally, lentiviral mediated transfer of shRNAs was an effective strategy for modifying gene expression in trophoblast cell lineages. This technique will permit the in vivo evaluation of “gain‐of‐function” and “loss‐of‐function” manipulations in the rat trophoblast cell lineage. genesis 47:433–439, 2009. © 2009 Wiley‐Liss, Inc.     

Shame,reputation and Egypt's lovers: A controversy over the nation's image

This paper addresses aspects of contemporary Egyptian nationalism by focusing on reactions within Egypt to a BBC‐produced documentary dealing with the life of a lower class Cairene woman. In particular, it explores how the notions of honor, shame and reputation are central to the construction and expression of this nationalistic voice, and how the concern over “Egypt's image” involves a contest over who is to represent the nation. This article argues that the contemporary nationalistic voice, championed by the urban middle classes, expresses an ambivalent attitude to both “the West” and “the Egyptian people”, and that it necessarily involves the hiding and undermining of subordinate social groups.     

Higher contribution of globally rare bacterial taxa reflects environmental transitions across the surface ocean

Microbial taxa range from being ubiquitous and abundant across space to extremely rare and endemic, depending on their ecophysiology and on different processes acting locally or regionally. However, little is known about how cosmopolitan or rare taxa combine to constitute communities and whether environmental variations promote changes in their relative abundances. Here we identified the Spatial Abundance Distribution (SpAD) of individual prokaryotic taxa (16S rDNA‐defined Operational Taxonomic Units, OTUs) across 108 globally‐distributed surface ocean stations. We grouped taxa based on their SpAD shape (“normal‐like”‐ abundant and ubiquitous; “logistic”‐ globally rare, present in few sites; and “bimodal”‐ abundant only in certain oceanic regions), and investigated how the abundance of these three categories relates to environmental gradients. Most surface assemblages were numerically dominated by a few cosmopolitan “normal‐like” OTUs, yet there was a gradual shift towards assemblages dominated by “logistic” taxa in specific areas with productivity and temperature differing the most from the average conditions in the sampled stations. When we performed the SpAD categorization including additional habitats (deeper layers and particles of varying sizes), the SpAD of many OTUs changed towards fewer “normal‐like” shapes, and OTUs categorized as globally rare in the surface ocean became abundant. This suggests that understanding the mechanisms behind microbial rarity and dominance requires expanding the context of study beyond local communities and single habitats. We show that marine bacterial communities comprise taxa displaying a continuum of SpADs, and that variations in their abundances can be linked to habitat transitions or barriers that delimit the distribution of community members.