烧伤狗浆血游离氨基酸的动态变化

烧伤狗浆血游离氨基酸的动态变化黎君友,周幼勤,赖业馥,赵有,伊少杰ＳｅｑｕｅｎｔｉａｌＣｈａｎｇｅｓｉｎＰｌａｓｍａＦｒｅｅＡｍｉｎｏＡｃｉｄＣｏｎｃｅｎｔｒａｔｉｏｎｉｎＢｕｒｎｅｄＤｏｇｓ￥Ｌｉｊｕｎｙｏｎ;Ｚｈｏｕｙｏｕｑｉｎ;Ｌａｉｙｅｆｕ;...     

内分泌疾病血浆氨基酸的模式

内分泌疾病血浆氨基酸的模式蒋滢,黄美英,何达纯ＰｌａｓｍａＦｒｅｅＡｍｉｎｏＡｃｉｄｉｎＥｎｄｏｃｒｉｎｅＤｉｓｅａｓｅｓ￥ＪｉａｎｇＹｉｎｇ;ＨｉｉａｎｇＭｅｉｙｉｎｇａｎｄＨｅＤａｃｈｕｎ（ＤｅｐａｒｔｍｅｎｔｏｆＢｉｏｃｈｅｍｉｓｔｒｙ,Ｓｕｚ...     

计时尺－氨基酸分析者的好帮手

计时尺－氨基酸分析者的好帮手赵建幸,刘玉群,陈伟ＴｉｍｅＲｕｌｅｒ──ａＧｏｏｄＦｅｌｌｏｗｆｏｒＡｍｉｎｏＡｃｉｄＡｎａｌｙｓｔ￥ＺｈａｏＪｉａｎｘｉｎｇ;ＬｉｕＹｕｑｕｎ;ＣｈｅｎＷｅｉ在我国的氨基酸分析仪器中,日立８３５系列占有一定比例,该仪器...     

转基因鱼构建模式的研究II.构建转基因鱼的几个影响因素(英文)

ＳｔｕｄｉｅｓｏｎＰａｔｅｒｎｏｆＰｒｏｄｕｃｉｎｇＴｒａｎｓｇｅｎｉｃＦｉｓｈＩ．ＳｅｖｅｒａｌＥｌｅｍｅｎｔｓＲｅｌａｔｅｄｔｏｔｈｅＥｓｔａｂｌｉｓｈｍｅｎｔｏｆＴｒａｎｓｇｅｎｉｃＦｉｓｈＬＩＵＣｈｕｎｑｉａｏ,ＺＨＡＮＧＹｏｎｇｚｈｏｎ...     

Overproduction and purification of Escherichia colitRNA~(Leu)

ＡｓａｎａｄａｐｔｅｒｍｏｌｅｃｕｌｅｂｅｔｗｅｅｎｍＲＮＡａｎｄａｍｉｎｏａｃｉｄ,ｔＲＮＡｐｌａｙｓａｃｒｕｃｉａｌｉｎｔｅｒｍｅｄｉａｔｅｒｏｌｅｉｎｐｒｏｔｅｉｎｂｉｏｓｙｎｔｈｅｓｉｓ.ＴｈｅｉｎｔｅｒａｃｔｉｏｎｂｅｔｗｅｅｎｔＲＮＡａｎｄａｍｉｎｏａｃｙｌｔＲＮＡｓｙｎｔｈｅｔａｓｅ(ａａＲＳ)ｉｓｔｈｅｐｒｅｒｅｑｕｉｓｉｔｅｆｏｒａｃｃｕｒａｔｅｔｒａｎｓｌａｔｉｏｎ.Ｒｅｓｕｌｔｓｏｆｉｎｖｉｖｏａｎｄｉｎｖｉｔｒｏｅｘｐｅｒｉｍｅｎｔｓｉｎｄｉｃａｔｅｔｈａｔｔｈｅｓｐｅｃｉｆ…     

慢性肾炎体液氨基酸与正常比较

慢性肾炎体液氨基酸与正常比较蒋滢,黄美英,何达纯ＣｈａｎｇｅｏｆＦｌｕｉｄＦｒｅｅＡｍｉｎｏＡｃｉｄｉｎＰａｔｉｅｎｔｓＷｉｔｈＣｈｒｏｎｉｃＮｅｐｈｒｉｔｉｓ￥ＪｉａｎｇＹｉｎｇ;ＨｅＤａｃｈｕｎａｎｄＨｕａｎｇＭｅｉｙｉｎｇ（Ｄｅｐａｒｔｍｅｎｔ...     

天然药物信息(医学期刊扫描:文摘和评论)(英文)

ＮａｔｕｒａｌＭｅｄｉｃｉｎｅＩｎｆｏｒｍａｔｉｏｎ(ＭｅｄｉｃａｌＪｏｕｒｎａｌＷａｔｃｈ :ＡｂｓｔｒａｃｔａｎｄＣｏｍｍｅｎｔｓ)Ｌ ＣａｒｎｉｔｉｎｅＩｍｐｒｏｖｅｓＥｘｅｒｃｉｓｅＴｏｌｅｒａｎｃｅＡｍｏｎｇＡｎｇｉｎａＰａｔｉｅｎｔｓｌｙｅｒＲＮ ,ＫｈａｎＡＡ ,ｅｔａｌ.Ｌ ｃａｒｎｉｔｉｎｅｍｏｄｅｒａｔｅｌｙｉｍｐｒｏｖｅｓｔｈｅｅｘｅｒｃｉｓｅｔｏｌｅｒａｎｃｅｉｎｃｈｒｏｎｉｃｓｔａｂｌｅａｎｇｉｎａ .ＪＡｓｓｏｃＰｈｙｓＩｎ ｄｉａ 4 8(11) :10 5 0～ 10 5 2 ,2 0 0 0 .Ｆｏｒｔｙ ｓ…     

中国五加科Song木属一些分类群的订正

在全面修订五加科Ｓｏｎｇ木属的基础上,对中国木几个问题的种,即Ａ．ｃｈｉｍｅｎｓｉｓ Ｌ．,Ａ．ｄｅｃａ８ｉｓｎｅａｎａ Ｈａｎｃｅ,Ａ．ｅｌａｔａ（Ｍｉｑ．）Ｓｅｅｍ．,Ａ．ｓｔｉｐｕｌａｔａ Ｆｒａｎｃｈ．,Ａ．ｄａｓｙｐｈｙｌｌｏｉｄｅａ Ｊ．Ｗｅｎ,Ａ．ｔｈｏｍｓｏｎｉｉ Ｓｅｅｎ．ｅｘ Ｃｌａｒｋｅ．Ａ．ｖｉｅｔｎａｍｅｎｓｉｓ Ｈａ,Ａ．ｆｏｌｉｌｏｓａ（Ｗａｌｌ．）Ｓｅｅｍ．,Ａ．ａ     

淡水瓦氏雅罗鱼基因文库的构建和抗冻蛋白基因同源序列的筛选

极地海域中的鱼类非常耐寒。它们的血液里常含有两种能降低其体内冰点的蛋白质：抗冻糖蛋白（ＡＦＧＰ）和不含糖的抗冻蛋白或抗冻多肽（ＡＦＰ）。这些蛋白的编码基因已经被克隆,并将黄盖鲽ＡＦＰ导入虹鳟鱼和大西洋鲑鱼,而改变了它们的抗冻性能。为开展中国的抗冻转基因鱼研究,本项目对中国北方的耐寒淡水鱼的抗冻基因进行了分离研究。所用实验材料鱼为中国内蒙古北部生活的瓦氏雅罗鱼（Ｌｅｕｃｉｓｃｕｓｗａｌｅｃｋｉｉ）,属鲤形目（Ｌｙｐｃｉｎｉｆｏｒｍｅｓ）,鲤科（Ｌｙｐｒｉｎｉｄａｅ）,雅罗鱼亚科（Ｌｅｕｃｉｓｉｎａｅ）。基本按标准方法构建瓦氏雅罗鱼的基因文库,只在包装反应至３０分钟时,再加一份ＢＨＢ２６９０制备物,并延长３０分钟反应时间。所得文库的成斑率大于１×１０５ｐｆｕ。已知ＡＦＰ基因常常多至４０个拷贝,故已够用。采用通用的原位分子杂交和Ｓｏｕｔｈｅｒｎ分析进行瓦氏雅罗鱼ＡＦＰ基因同源序列的分离及其亚克隆分析。以北美黄盖鲽ＡＦＰ基因片段为探针,从瓦氏雅罗鱼ＤＮＡ文库中筛选了三个阳性克隆。图一所示为其中一个的双份膜及其复筛结果。图二为这个阳性克隆ＤＮＡ经过三种限制酶切后,同时与北美黄盖鲽ＡＦＰ基因片段和该基因０．４ＫｂｃＤＮ     

峨眉山艾纳香属一新种

峨眉山艾纳香属一新种祝正银（四川省中药学校,四川峨眉山６１４２０１）ＡＮＥＷＳＰＥＣＩＥＳＯＦＢＬＵＭＥＡＦＲＯＭＥＭＥＩＳＨＡＮＺｈｕＺｈｅｎｇｙｉｎ（ＳｉｃｈｕａｎｇｃｈｏｌｏｆＣｈｉｎｅｓｅＭａｔｅｒｉａＭｅｄｉｃａ,Ｅｍｅｉｓｈａｎ,Ｓｉｃｈ...     

Evaluation of an alternative to feeding whole frozen fish in belugas (Delphinapterus leucas)

Feeding fish to captive piscivores can be challenging owing to cost, availability, variability in nutrient, and caloric composition, as well as handling and storage concerns. This trial evaluated the response of three belugas to being fed Fish Analog, an alternative to frozen fish. Body condition, gut transit time, serum chemistry and metabolic hormone analytes, immune function, and behavioral motivation were the dependent variables. Belugas (n=3) were fed various levels of Fish Analog (0-50%) over a 6-month period, and follow-up studies were conducted to further examine several dependent variables. When provided in gradually increasing amounts, belugas consumed the Fish Analog, with only minor fecal consistency changes and without behavioral responses indicative of gastric discomfort. Axillary girth and blubber thickness were positively correlated, and did not differ significantly with changes in the percentage of Fish Analog fed. Individual animal variation in initial passage time, some serum chemistry analytes, and immune function differences were noted following feeding of Fish Analog. Feeding Fish Analog reduced blood n9 fatty acids compared with captive belugas fed no Fish Analog. Feeding a DHA-enriched Fish Analog increased several n3 fatty acids, including eicosapentaenoic acid, but not DHA, compared with whales fed no Fish Analog or non-DHA-enriched Fish Analog. Fish Analog was shown to be a viable alternative to feeding fish at up to 50% of the dietary caloric density.     

The adaptor protein fish associates with members of the ADAMs family and localizes to podosomes of Src-transformed cells

Fish is a scaffolding protein and Src substrate. It contains an amino-terminal Phox homology (PX) domain and five Src homology 3 (SH3) domains, as well as multiple motifs for binding both SH2 and SH3 domain-containing proteins. We have determined that the PX domain of Fish binds 3-phosphorylated phosphatidylinositols (including phosphatidylinositol 3-phosphate and phosphatidylinositol 3,4-bisphosphate). Consistent with this, a fusion protein of green fluorescent protein and the Fish PX domain localized to punctate structures similar to endosomes in normal fibroblasts. However, the full-length Fish protein was largely cytoplasmic, suggesting that its PX domain may not be able to make intermolecular interactions in unstimulated cells. In Src-transformed cells, we observed a dramatic re-localization of some Fish molecules to actin-rich structures called podosomes; the PX domain was both necessary and sufficient to effect this translocation. We used a phage display screen with the fifth SH3 domain of Fish and isolated ADAM19 as a binding partner. Subsequent analyses in mammalian cells demonstrated that Fish interacts with several members of the ADAMs family, including ADAMs 12, 15, and 19. In Src-transformed cells, ADAM12 co-localized with Fish in podosomes. Because members of the ADAMs family have been implicated in growth factor processing, as well as cell adhesion and motility, Fish could be acting as an adaptor molecule that allows Src to impinge on these processes.     

Short Notices

Burkardt, Nina. (1990) Opportunities to Protect Instream Flows and Wetland Uses of Water in Florida. U.S. Fish and Wildlife Service, Biological Report
Wolff S.W., Wesche T.A., Harris D.D. & Hubert W.A. (1990) Brown trout population and habitat changes associated with increased minimum low flows in Douglas Creek, Wyoming. U.S. Fish and Wildlife Service, Biological Report
Clark Donald R. Jr (1990) Dicofol (Kelthane) as an Environmental Contaminant: A Review. U.S. Fish and Wildlife Service, Fish and Wildlife Technical Report
Todd Thomas N. (1990) Genetic differentiation of walleye stocks in Lake St. Clair and western Lake Erie. United States Department of the Interior Fish and Wildlife Service. Fish and Wildlife Technical Report
Hudson Patrick L., Lenat David R., Caldwell Broughton A. & Smith David (1990) Chiro-nomidae of the Southeastern United States: a checklist of species and notes on biology, distribution and habitat. United States Department of the Interior Fish and Wildlife Service. Fish and Wildlife Research
Eisler Ronald (1990) Chlordane hazards to fish, wildlife and invertebrates: a synoptic review. United States Department of the Interior Fish and Wildlife Service. Biological Report
Eisler Ronald (1990) Paraquat hazards to fish, wildlife and invertebrates: a synoptic review. United States Department of the Interior Fish and Wildlife Service. Biological Report
Gopal Brij. (Ed.) (1990) Ecology and Management of Aquatic Vegetation in the Indian Subcontinent     

A new method for isolating tyrosine kinase substrates used to identify fish, an SH3 and PX domain-containing protein, and Src substrate.

We describe a method for identifying tyrosine kinase substrates using anti-phosphotyrosine antibodies to screen tyrosine-phosphorylated cDNA expression libraries. Several potential Src substrates were identified including Fish, which has five SH3 domains and a recently discovered phox homology (PX) domain. Fish is tyrosine-phosphorylated in Src-transformed fibroblasts (suggesting that it is a target of Src in vivo) and in normal cells following treatment with several growth factors. Treatment of cells with cytochalasin D also resulted in rapid tyrosine phosphorylation of Fish, concomitant with activation of Src. These data suggest that Fish is involved in signalling by tyrosine kinases, and imply a specialized role in the actin cytoskeleton.     

Evidence for local adaptation in neighbouring Daphnia populations: a laboratory transplant experiment

1. We tested whether two neighbouring Daphnia galeata populations (from Lake Blankaart and Fish Pond), separated only by 5 m of land and with the occasional exchange of water were genetically differentiated in allozyme markers and life history traits. Allozyme electrophoresis revealed that the populations differed in allelic as well as in genotypic composition. 2. In a laboratory transplant experiment, in which animals of four clones of each of the populations were raised in the water of both ponds, survival in Blankaart water was high for both the Blankaart and Fish Pond clones, whereas survival in Fish Pond water was high for the Fish Pond clones, but low for the Blankaart clones. 3. Fish Pond clones produced fewer neonates than Blankaart clones when cultured in Blankaart water. High egg mortality was observed for animals that were raised in Blankaart water, and this egg mortality was higher for Fish Pond clones than for Blankaart clones. 4. Our results provide evidence for genetic differentiation between Daphnia populations inhabiting neighbouring water bodies and suggest local adaptation to environmental conditions other than direct predation.     

A global review and meta-analysis of applications of the freshwater Fish Invasiveness Screening Kit

Reviews in Fish Biology and Fisheries - The freshwater Fish Invasiveness Screening Kit (FISK) has been applied in 35 risk assessment areas in 45 countries across the six inhabited continents (11...     

Food selection by brook trout in a subalpine stream

The diet and food selection of brook trout (Salvelinus fontinalis) and macroinvertebrate drift are described for a high altitude stream (3 205 m above mean sea level) adjacent to the alpine zone on the Snowy Range in the Medicine Bow National Forest, Wyoming. Diet composition differed between brook trout of two length classes, 150 mm and > 150 mm in total length. The number of prey organisms per stomach generally declined and the proportion of terrestrial prey items increased from July to September, 1985. Fish of both length classes selected for Trichoptera and Coleoptera over the three sampling months, and fish > 150 mm also selected for terrestrial insects in August and September.The Unit is jointly supported by the University of Wyoming, the Wyoming Game and Fish Department, and the US Fish and Wildlife ServiceThe Unit is jointly supported by the University of Wyoming, the Wyoming Game and Fish Department, and the US Fish and Wildlife Service     

Fish telemetry in African inland waters and its use in management: a review

Reviews in Fish Biology and Fisheries - Fish telemetry is a widely established technique in developed countries. However, in underdeveloped regions, its use is generally lacking. We briefly present...     

Emerging from the murk: threats,challenges and opportunities for the global swim bladder trade

Reviews in Fish Biology and Fisheries - Fish maw, or swim bladder, has long been used as food and medicine in Asia, particularly in southern China. Considering its history as luxury and nutritious...