Immunohistochemical mapping of galanin-like neurons in the rat central nervous system

Using an antiserum generated in rabbits against synthetic galanin (GA) and the indirect immunofluorescence method, the distribution of GA-like immunoreactive cell bodies and nerve fibers was studied in the rat central nervous system (CNS) and a detailed stereotaxic atlas of GA-like neurons was prepared. GA-like immunoreactivity was widely distributed in the rat CNS. Appreciable numbers of GA-positive cell bodies were observed in the rostral cingulate and medial prefrontal cortex, the nucleus interstitialis striae terminalis, the caudate, medial preoptic, preoptic periventricular, and preoptic suprachiasmatic nuclei, the medial forebrain bundle, the supraoptic, the hypothalamic periventricular, the paraventricular, the arcuate, dorsomedial, perifornical, thalamic periventricular, anterior dorsal and lateral thalamic nuclei, medial and central amygdaloid nuclei, dorsal and ventral premamillary nuclei, at the base of the hypothalamus, in the central gray matter, the hippocampus, the dorsal and caudoventral raphe nuclei, the interpeduncular nucleus, the locus coeruleus, ventral parabrachial, solitarii and commissuralis nuclei, in the A1, C1 and A4 catechaolamine areas, the posterior area postrema and the trigeminal and dorsal root ganglia. Fibers were generally seen where cell bodies were observed. Very dense fiber bundles were noted in the septohypothalamic tract, the preoptic area, in the hypothalamus, the habenula and the thalamic periventricular nucleus, in the ventral hippocampus, parts of the reticular formation, in the locus coeruleus, the dorsal parabrachial area, the nucleus and tract of the spinal trigeminal area and the substantia gelatinosa, the superficial layers of the spinal cord and the posterior lobe of the pituitary. The localization of the GA-like immunoreactivity in the locus coeruleus suggests a partial coexistence with catecholaminergic neurons as well as a possible involvement of the GA-like peptide in a neuroregulatory role.     

烟酰胺腺嘌呤二核苷酸激酶研究进展

本文介绍了ＮＡＤ￣＋激酶的来源和分布、测定方法、最适ｐＨ、反应平衡常数、影响酶稳定性的因子、比活力和动力学常数、三磷酸核苷酸特异性、对金属离子的需要、抑制剂和底物类似物、反应机制、反应的感光性、酶固定化、与钙调蛋白的关系、各种效应物等方面研究的进展,并进行了评述。     

小鼠基因组中的微卫星重复序列的数量、分布和密度

作者分析了老鼠基因组中各染色体及其内含子、外显子和基因间区上各种类型的微卫星(1-6个碱基的重复序列)的数量及其密度。SSR约占老鼠基因组的2.85%,其中46.2%存在于基因间区,4.75%存在于外显子,49.05%在内含子区域,即非编码区富含微卫星。微卫星的数量与染色体或基因区域的大小有关,但密度与染色体或基因区域的大小的关系并不十分密切。第4染色体的外显子区域中6种类型的SSR含量都比其它区域少。A,T,AC,AG,AT,AAC,AAG,AGG,AAAC,AAAG,AAAT,AACC,AAAAC,AAAAG,AAAAT,AAACC,AAAGG,AAGAG,AAAAAC,AAAAAG,AAAAAT,AAAGAG,ACACAT,ACAGAG,ACAGGC,ACATAT是老鼠基因组中主要的SSR类型,而一些5碱基重复单元的SSR在老鼠基因组的某一条甚至某几条染色体都不存在     

奉节脐橙不同器官元素含量分布特征

对奉节脐橙果、叶、干、根中的必需大量元素 (N、K、Ca、Mg、P、S)、微量元素 (Fe、Mn、Cu、Zn、B、Mo)、有益元素 (Si、Co、F、Se、Ni、Sr)、有毒有害元素 (Hg、As、Cr、Cd、Pb、Sb、Bi)的分布特征进行分析 ,结果表明 :果、叶、干、根对各元素的吸收与富集特征具有明显的规律性 ,如叶、干、根中大量元素含量次序均为 Ca>N>K>Mg,叶、干、根中微量元素含量均有 Fe>Mn>Zn>Cu>Mo的规律 ,根、干、叶中有益微量元素含量次序均为 Si>Sr>F>Ni>Co>Se,叶、干、根中有毒有害元素含量次序均有 Pb>As>Sb >Hg的规律 ;在脐橙各器官中 ,叶是大量元素与有毒有害元素最富集的器官 ,根则是有益元素最富集的器官 ,有毒有害元素的 Cr、微量元素的 Mn、Fe、Mo也以根最为富集 ,干则是上述元素最不富集的器官 ,所有元素中 ,仅 Zn在干中最富集 ;大量元素 (除 N外 )、微量元素、有益元素在脐橙各器官的分配含量与地壳土壤背景值的分异含量趋势基本一致 ,土壤背景是这三类元素的主要控制因素。但有毒有害元素的分配形式则明显受人类活动的影响     

海南岛新生代种子植物区系科的多样性分析

根据化石及现代植物资料,海南岛第三纪种子植物有53科,其中古近纪已出现48科,新近纪降至25科,第四纪晚期有89科,现代增加至233科.区系地理成分分析结果表明,海南岛古近纪时,泛热带科占当时科总数的45.8%,热带-亚热带科占8.3%,亚热带-温带科占31.3%,其中出现了很多海南岛现代植物区系的表征科,如冬青科、壳斗科、金缕梅科、樟科、桃金娘科、罗汉松科、棕榈科、红树科、梧桐科和松科等,基本形成了现代海南岛植物区系的雏形.新近纪时由于全球气候变冷,本区植物区系组成大幅度减少,热带性质也明显减弱,其中泛热带分布的科仅占当时海南岛植物区系全部科的32.0%,热带-亚热带分布的科占12.0%,而亚热带-温带分布的科所占比例有所升高,达36.0%.第四纪晚期至现代,随着气候逐渐回暖,本区植物区系的热带性质逐渐加强.第四纪晚期泛热带科占当时科总数的49.4%,热带-亚热带科占12.4%,亚热带-温带科占19.1%,区系特征逐渐接近现代.现代海南种子植物区系中,泛热带分布科占科总数48.1%,热带-亚热带科占18.9%,亚热带-温带科占15.9%.总体上,海南岛第三纪早期的古近纪、第四纪晚期一直到现代均以泛热带分布的科占优势,而第三纪晚期的新近纪本区植物区系的热带性质较弱.整体上反映出海南岛新生代种子植物区系的演变特征与古近纪以来全球气候的变化有密切的联系.     

From the creation of the Veterinary Schools to the evolution of the notion of contagion in the 19th and 20th centuries

The creation of the Veterinary Schools in the 18th century would reveal a plethora of scientists, some of whom would be the precursors of Pasteur, some rivals, others followers collaborators or friends of the Master. Among the precursors let us name Chabbert, Huzard, Girard, Delafond, Renault, Toussaint, Galtier ; among the rivals: Chauveau, Arloing, Cornevin and Thomas; among the followers, collaborators or friends of Pasteur: Bouley, at first a resolute spontaneist, then the most fervent in defense of Pasteur (President of the Academy of Medicine and of the Academy of Sciences) and Nocard, Director of the School in Alfort, an important collaborator of Pasteur. Later, there was Leclainche, who created the International Office of Epizootics, and who was President of the Academy of Sciences; Guérin, who with Calmette developed the BCG vaccination; Ramon, the father of anatoxins (vaccines against diphtheria, and tetanus, combined vaccines, adjuvants to immunity). Thus, the creation of the Veterinary Schools contributed not only to the evolution of the notion of contagion, to the amelioration of animal health and the economics of agricultural production, but also to serious advances in human care, and to the protection of public health.     

土鳖虫与其混淆品的微性状鉴别研究

为对目前药材市场流通的中药材土鳖虫进行微性状特征研究,探究土鳖虫与其混淆品的区别。实验通过查阅资料,对市场土鳖虫品种进行调查,运用中药微性状鉴定法对土鳖虫的不同部位采用体视显微镜、生物显微镜、扫描仪等仪器进行图像采集,并利用Photoshop CS5软件景深合成高清晰度微性状特征图片来对其进行鉴别研究。实验结果表明,土鳖虫口器、腹背板边缘、肛上板、生殖板、触角、单眼间距与复眼间距比例、单眼、复眼、前足胫节、跗节、爪、尾须、背甲及腹甲刚毛等方面与混淆品有比较明显的区别。     

Characterization of suspended solids in Lake Biwa by measuring their elemental composition of Al,Si, P,S, K,Ca, Ti,Mn, and Fe

We investigated the concentrations of particulate Al, Si, P, S, K, Ca, Ti, Mn, and Fe at various areas of Lake Biwa, such as the limnetic zone in the northern basin, the offshore zone, the dredged area and Akanoi Bay in the southern basin, and reed community areas in the shore. In the wide and deep northern basin, the density of suspended solids was low, and substantial fractions of particulate Si, P, S, and Ca were biogenic matter produced by phytoplankton. In the offshore zone in the narrow and shallow southern basin, the concentration ratios of particulate Si, K, Ca, Ti, and Fe against particulate Al were almost constant throughout the year and were similar to those found in crusts. Therefore, these elements are most likely derived from terrigenous clay matter. In the dredged area where the hypolimnion became anoxic during the stratification period, the concentrations of particulate Fe, Mn, P, S, and Ca increased because of the formation of hydrous Fe and Mn oxides and Fe sulfide. In Akanoi Bay, the ratios of Mn/Al and Fe/Al were high because of the stirring of sediments enriched with Mn and Fe oxides. In the reed community areas, the concentration of suspended solids correlated well with particulate Fe and P, but not with Al or chlorophyll a; therefore these areas seem to be rich in humic organic matter enriched with P and Fe due to microbial activity. Thus, the elemental composition of suspended solids reflects the chemical, biological, and physical processes in the lake and gives us useful indices of the characteristics of the water. Received: February 5, 2001 / Accepted: August 28, 2001     

Early Cretaceous Palynofloras from Tarim Basin,Xinjiang

One hundred and eight species of fossil spores and pollen referred to 55 genera found from the Lower Cretaceous Kapushaliang (Kizilsu) Group in the Tarim Basin of Xinjiang have been studied in this paper. The Kapushaliang Group may be divided into the Yageliemu Formation, the Shushanhe Formation and Baxigai Formation in ascending order. The Yageliemu palynoflora is characterized by the predominance of gymnospermous pollen, the relative abundance of pteridophytic spores and the absence of angiospermous pollen. Coniferae is dominant, while Lygodiaceae and Schizaeaceae are important in the flora. The most common species of palynomorphs are Todisporites minor, Cicatricosisporites dorogensis, C. australiensis, Lygodiumsporites subsimplex, Schizaeoisporites cretacius, S. zizyphinus, S. certus. Cibotiumspora paradoxa, Cyathidites australis, C. minor, Biretisporites potoniael, Cycadopites minimus, Pseudowalchia hiangulina, Parvisaccites radiatus, Podocarpidites multesimus, P. canadensis, Alisporites bilateralis, Cedripites cretaceus, C. canadenris, Abietineaepollenites microalatus, Clarsopollis classoides, C annulatus, C. xinjiangensis (sp. nov.), Ephedripites multicostatus, E. tarimensis (sp. nov.), etc . The palynoflora is comparable with those of the lower part of the Lower Xinminbu Formation in northwestern China and the lower part of the Lower Mannville Formation in western Canada, which is considered corresponding from Berriasian to Valanginian in age. The Shushanhe palynoflora is also characterized by dominance of gymnosperms and subdominance of pteridophyta. Some primitive angiospermons pollen grains are found in the upper part of the formation. In addition to the most common species of the above mentioned palynoflora, the important species in the present palynoflora comprise Cicatricosisporites potomacensis, C. orbiculatus, C. subrotundus, Concavissimisporites punctatus, C. verrucosus, C. globosus, lmpardecispora apiverrucata, Trilobosporites crassus, T. trioreticulosus, Pilosisporites verus, P. trichopapillosus, Klukisporites pseudoreticulatus, Verrucosisporites obscurilaesuratur, Converrucosisporites saskatchewanensis, Foraminisporis wonthaggiensis, Cingulatisporites valdensis, C. ruginosus, Jiaohepollis verus, Pityosporites constrictus, etc. More than 40 in dicative species of Early Cretaceous occur in the assemblage. The palynoflora compares clcsely with those of the middle part of the Lower Xinminbu Formation and the Quartz Sand Member of the Lower Mannville Formatton. It is suggested that the Shushanhe Formation should belong to Hauterivian of Barremian in age. The Baxigai palynoflora is basically similar to the Shushanhe palynoflora. But the palynoflora shows an obvious increase in abundance of lygodiaceous spores and angiospermous pollen accompanied by a great reduction of Classopollis and Ephedripltes. Most of the Early Cretaceous species occurring in the above mentioned palynofloras are present. In addition, some Cretaceous species recorded from Aptian and Albian Stages in Europe, North America and Australia, such as Trilobosporites tribotrys, T. trioreticulosus, Coptospora paradoxa, Camarozonosporites insignis, Crybelosporites punctatus, etc., appear in the assemblage. In comparison with the microfloras of the upper part of the Lower Xinminbu Formation, the Upper Mannville Formation and the lower part of the Lower Colorado Group, the Baxigai Forma- tion should be referred to Aptian to Albian in age. Judging from the palynofloras, the early Cretaceous palaeoclimate in the basin should belong to the arid or semi-arid type of the subtropic zone. However, from Barremtan through Albian the climatic conditions might appear relatively wet.     

Petroleum Sporo-Pollen Assemblage and Oil Source Rock of Yecheng Seg in Xinjiang

Forty-seven species of fossil pollens and spores referred to 33 genera extracted from the crude oil samples collected from the Yecheng Seg of the Talimu Basin in Xinjiang were observed. Based on the investigation of the characteristics of the petroleum sporo-pollen assemblage, the problem on petroleum source of this oil-bearing region is discussed. The principle and method to judge source rock from petroleum sporo-pollen assemblage are specially explained in this paper. The petroleum sporo-pollen assemblage of the Neogene reservoir of the Yecheng Seg consists of Deltoidospora perpusila, Deltoidospora gradata, Cyathidites australis, Cyathidites minor, Biretisporites potoniaei, Dictyophyllidites harrisii, Dictyophyllum rugosum, Cibotiumspora paradoxa, Gleicheniidites senonicus, Gleicheniidites rousei, Undula- tisporites concave, Lycopodiumsporites sp., Osmundacidites wellmanii, Leptolepidites major, Apiculatisporis ovalis, Bennettiteaepollenites lucifer, Cycadopites typicus, Cyeadopites nitidus, Cycadopites minimus, Cycadopites carpentieri, Cycadopites follicularis, Chasmatosporites elegans, Classopollis classoides, Classopollis annulatus, Podocarpidites multesimus, Podocarpidites major, Parvisaccites enigmatus, Quadraeeulina limbata, Caytonipollenites pallidus, PteruchipoUenites thomasii, Alisporites grandis, Alisporites bilateralis, AbietineaepoUenites microalatus, Abietineaepollenites minimus, Pinuspollenites sp., Piceaepollenites sp., Cedripites st)., Ephedripites sp., Eucommiidites troedssonii, Magnolipollis neogenicus, Quercoidites microhenrici, Chenopodipollis multiplex, Artemisiae-pollenites sellularis, etc . This is a typical composite type of petroleum sporo-pollen assemblage, which eomprises the Jurassic species, the Cretaceous species, the Eogene species and the Neogene species of spores an, d pollen. In accordance with the characteristics of the above mentioned petroleum sporopollen assemblage, the source rock of the Yecheng Seg is considered to be Jurassic Period in age, and the black shales and mudstones of the Middle Jurassic Yangye-Taerga Formation are judged to be the most favorable source rocks in the region.     

基于GGE双标图的棉花品种生态区划分

采用遗传力校正的GGE双标图分析方法,对以安庆、南阳、黄冈、荆州、武汉、襄阳、常德、岳阳、南京、南通、盐城、九江、简阳、射洪和慈溪等15个试验点为代表的长江流域棉区,基于皮棉产量进行品种生态区划分,并对划分结果进行信息比（IR）校正,为长江流域棉区棉花品种的选择提供科学依据.结果表明： 长江流域棉区可以划分为3个棉花品种生态区,即以简阳和射洪为代表的“四川盆地棉花品种生态区”、以南阳和襄阳为代表的“南襄盆地棉花品种生态区”和涵盖其余试验点的“长江流域主体棉花品种生态区”.
     

Adsorption of monovalent cations to bilayer membranes containing negative phospholipids.

The electrophoretic mobilities of multilamellar phosphatidylserine vesicles were measured in solutions containing monovalent cations, and the xi potentials, the electrostatic potentials at the hydrodynamic plane of shear, were calculated from the Helmholtz--Smoluchowski equation. In the presence of 0.1 M lithium, sodium, ammonium, potassium, rubidium, cesium, tetraethylammonium, and tetramethylammonium chloride, the xi potentials were -60, -62, -72, -73, -77, -80, -82, and -91 mV, respectively. Similar results were obtained with phosphatidylglycerol vesicles; different results were obtained with cardiolipin, phosphatidylinositol, and phosphatidic acid vesicles. The phosphatidylserine results are interpreted in terms of the Stern equation, a combination of the Gouy equation from the theory of the diffuse double layer, the Boltzmann relation, and the Langmuir adsorption isotherm. Evidence is presented that suggests the hydrodynamic plane of shear is 2 A from the surface of the membrane in solutions containing the alkali metal cations. With this assumption, the intrinsic association constants of the above monovalent cations with phosphatidylserine are 0.8, 0.6, 0.17, 0.15, 0.08, 0.05, 0.03, and 0 M-1, respectively. The validity of this approach was tested in two ways. First, the xi potentials of vesicles formed from mixtures of phosphatidylserine and a zwitterionic lipid, phosphatidylcholine, were measured in solutions containing different concentrations of sodium. All the data could be described by the Stern equation if the "relaxation" of the ionic atmosphere, which is predicted by classic electrostatic and hydrodynamic theory to occur at low salt concentrations and high potentials, was circumvented by using only large (diameter greater than 13 micrometers) vesicles for these measurements. Second, the fluorescent probe 2-(p-toluidinyl)naphthalene-6-sulfonate was used to estimate the potential at the surface of phosphatidylserine and phosphatidylglycerol vesicles sonicated in 0.1 M NaCl. Reasonable agreement with the predicted values of the surface potential was obtained.     

Comparative histochemical distribution of glycogen and alkaline phosphatases in the placenta

Summary The distribution of glycogen, non-specific alkaline phosphatase, and specific phosphatases acting on adenosine monophosphate, adenosine triphosphate, inosine triphosphate, thiamine pyrophosphate, uridine diphosphate, fructose-6-phosphate, fructose-1:6-diphosphate, and glucose-6-phosphate is described in the placentae and accessory structures of the horse, sheep, cat, dog, ferret, rat, rabbit, guinea pig, and man, and in the yolk-sac of the chick, the oviviparous fish Limia maculata, and man.Correlation between the distribution of these substances and placental function is sought, and the results are discussed with respect to the trophoblast, decidua, giant cells, yolk-sac, non-placental chorion and maternal epithelium and uterine secretion, and allantois. The significance of the presence of the enzymes in the carnivore interstitial matrix, the ferret thickened endoderm and the rodent spongy zone is also considered.     

黑河中下游绿洲生态安全评价

生态安全包括自然生态安全、经济生态安全和社会生态安全 ,尤指自然和半自然生态系统的安全 ,即生态系统完整性和健康的整体水平反映。生态安全是维护地区或国家乃至全球的生态环境不受威胁的状态 ,能为整个生态经济系统的持续发展提供生态保障。从水安全、土地安全、经济社会安全出发 ,选取水资源量供需比、地表水质量、地下水埋深和地下水矿化度 4种水安全评价指标 ;土地人口承载度、林地覆盖率、草地载畜度、绿洲面积变化率、绿洲土地沙化率、绿洲土地盐碱化率和抗灾度等 7种土地安全评价指标 ;人均 GDP产值、单位面积农业生产总值、农民人均纯收入、绿洲人口密度、城市化率和文明度等经济社会安全评价指标 ;共计 17项 ,建立了绿洲生态安全评价综合指标体系。然后 ,应用综合评价法、层次分析法和 GIS等多种方法与手段 ,在绿洲尺度上对黑河干流中下游地区的张掖、临泽、高台、鼎新和额济纳 5个绿洲进行绿洲生态安全综合评价。评价结果显示 :自黑河中游至下游 ,各个绿洲的生态安全状况依次降低 ,最下游的额济纳绿洲生态安全仅为 4级的差状态。其中 ,水安全是关键 ,土地安全是载体 ,经济社会安全是保障 ,以上几方面相互关联 ,共同作用导致绿洲的生态安全综合水平。     

Compositional changes of the muscular layers of the stomach with aging

To elucidate compositional changes of the stomach with aging, the authors investigated age-related changes of the elements and relationships among the elements in the muscular layers of the pylorus, cardia, lesser curvature, and greater curvature by inductively coupled plasma-atomic emission spectrometry. After ordinary dissection by medical students, the pylori, cardias, lesser curvatures, and greater curvatures were removed from the subjects, consisting of 19 men and 1 woman, ranging in age from 65 to 95 yr. The muscular layers were isolated and the element contents were determined.The calcium content increased progressively with aging in the muscular layers of the pylorus, cardia, and lesser curvature, whereas it tended to increase in the muscular layer of the greater curvature with aging. Regarding sulfur, the content increased significantly in the muscular layer of the pylorus with aging, but not significantly in the muscular layers of the cardia, lesser curvature, and greater curvature with aging.Regarding the relationships among the elements, significant direct correlations were found among calcium, phosphorus, sulfur, and magnesium in both the muscular layers of the pylori and cardias, with some exceptions.     

基于遥感数据的晋陕蒙交汇区景观格局定量分析--以东胜幅1/25万TM影像分析为例

以1996年8月份东胜幅1/25万TM影像数据为例,借助Arc/Info和Arc/view软件,对其景观进行了分类,并形成景观分类图,共分为9类景观类型.用景观格局分析软件FRAGSTATS计算了各类景观参数,并从斑块、类型和景观3个水平上计算了相应的参数和景观指数,定量地揭示了整个景观格局与类型特征.并构建了反映景观破碎化程度的一个指数--斑块密度指数(PD2).     

Notulae de Ranunculaceis sinensibus (ⅩⅩⅢ)

(1) In this paper, differences among the five genera constituting the tribe Cimi cifugeae of the family Ranunculaceae are discussed. Beesia, the first genus, with compound cymes and flowers bearing neither petals nor staminodes, is different from the other four genera with simple or compound racemes and flowers bearing either petals or staminodes, and may occupy a primitive position within the tribe. As to the other four genera, Souliea is characterized by the stem without basal leaf but with 2～5 sheath-like cataphylls, the sepals being deciduous but not caducous, moderate in size and petaloid, the petals being much smaller than sepals, but pink in color and more or less petaloid, the pollen grains being pan tocolpate or pantoporate, the carpels being 1～3 per flower, when mature forming dry linear follicles conspicuously reticulate on the surface and dehiscent along the ventral suture, and the seeds being reticulate-foveolate on the surface. These diagnostic characters indicate clear ly that Souliea might have deviated from the lineage formed by the next three genera, i. e. Anemopsis, Cimicifuga, and Actaea, which have their own well-recognizable diagnostic characters. Anemopsis is characterized by the normally developed basal leaf, the racemose inflorescence with sparse and few long pedicellate flowers, the sepals 7～10 in number, mod erate in size, and petaloid, the petals slightly smaller than sepals, the tricolpate pollen grains, the carpels 2～4 per flower, stalked, when mature forming dry oblong follicles with transverse veins on the surface, and the seeds with scaly membranous wings. Cimicifuga is distinguished by the normally developed basal leaf, the caducous, small, often sepaloid sepa ls, the organs of the second floral whorl sometimes with empty sterile anthers being stamin odes not petals, the tricolpate pollen grains, the carpels 1～8 per flower, when mature form ing dry oblong or ovoid follicles with transverse veins on the surface, and the seeds usually with scaly membranous wings. The last genus Actaea is different by the basal leaf trans formed into a small scale, the caducous, small, often sepaloid sepals, the organs of the sec ond floral whorl being clawed petals, the pollen grains with 3(4～6) colpi, carpel 1 per flow er, when mature forming a fleshy indehiscent berry smooth on the surface and without any veins, the seeds roughish or slightly rugose, neither foveolate nor winged on the surface, and the advanced most asymmetric karyotype. According to the diagnostic characters given above, we believe that Beesia, Souliea, Anemopsis, Cimicifuga, and Actaea do represent five independent genera, and the treatment of the tribe Cimicifugeae including these five genera in it by Hutchinson (1923), Janchen (1949) and some other authors, has precisely shown the taxonomic diversity within the tribe. We are therefore unable to accept the treatment published by Compton et al. (1998) to lump the two genera, Souliea and Cimicifuga, into the genus Actaea. (2) Compton et al. (1998, 1997) found out that the Chinese plants previously identified by various authors as Cimicifuga foetida L., in which the terminal and lateral racemes of the compound raceme flower more or less simultaneously, differ from the true C. foetida L. in northern Asia, in which the terminal raceme of the compound raceme flowers before the lateral ones, and thus restored the species name Cimicifuga mairei Lévl. , which was formerly reduced to the synonymy of C. foetida L. , for the Chinese plants. After examining the specimens collected from Siberia and from Southwest China we failed to find out any other differences in both vegetative and reproductive organs between the plants of the two regions, and we consider that it is better to treat the populations in Southwest and Central China as a geographical variety of Cimicifuga foetida L. A new combination, Cimicifuga foetida L. var. mairei (Lévl.) W. T. Wang & Zh. Wang, is thus made. (3) 3 species of Delphinium, 1 species and 1 variety of Clematis are described as new.     

Comparative Evaluation of Trace Metals in the Blood of Hepatitis C Patients and Healthy Donors

Hepatitis C is one of the common types of chronic liver disease, and its plausible association with trace metal imbalance has been investigated in the present study. The blood samples of hepatitis C patients and healthy donors were analysed for trace metals (Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Na, Pb, and Zn) by flame atomic absorption spectrophotometry after wet acid digestion. In the blood of the hepatitis patients, mean concentrations of essential metals, Na, K, Fe, Ca, Mg, and Zn, were 918.7, 361.0, 102.5, 20.00, 24.66, and 9.429 ppm, respectively, while the mean metal levels in the blood of healthy donors were 1509, 406.8, 232.5, 28.35, 24.59, and 8.799 ppm, respectively. On the average, Cr was significantly higher, while Na, Fe, Ca, Cu, Mn, Pb, and Cd were comparatively lower in the blood of the patients. The correlation study manifested significantly divergent mutual relationships of trace metals in the blood of the patients and healthy donors. Multivariate statistical methods revealed considerably diverse distribution of trace metals in the two groups. Dissimilarity in the trace metal distribution was also noted with the gender and residential location of the donors in both groups.     

现代生物技术在乳品工业中的应用研究

随着细胞生物学和分子生物学的发展及对生物物理、生物化学、遗传学和免疫学研究的深入,培育了基因工程、细胞工程、酶工程、发酵工程等改变生物特性进行物质转化的现代生物技术,形成了DNA探针、PCR技术、分子标记、生物荧光技术、基因芯片技术等前沿性的生物检测技术,其在乳品工业中的广泛应用,推动了乳业的技术变革,对乳品生产、研究和乳品安全意义重大。     

Selenium Supplementation Changes the Ion Profile in the Pancreas of Chickens Treated with Cadmium

Increasing evidence indicates that selenium (Se) could antagonize metal toxicity, including cadmium (Cd) toxicity. However, the effects of Se on Cd-induced changes in the ion profile in the pancreas of chickens have not been reported. In the present study, 128 Hy-Line brown laying chickens were divided into the control group, Se-treated group, Se/Cd-treated group, and Cd-treated group, and we detected the concentrations of 28 ions in the four groups by inductively coupled plasma mass spectrometry. In the Cd-treated group, the accumulation of Cd in the pancreas was 836.8 times higher that than in the control group (27,353.71 ppb/32.69 ppb). Meanwhile, the Ca, Ti, Fe, Mo, Li, Al, and Pb levels increased and the Cr, Mn, Ni, Cu, Zn, Se, Sr, and Sb levels decreased due to sub-chronic Cd poisoning. The Fe, Mo, Ba, and Pb levels decreased in the Se/Cd-treated group. Our findings suggest that Cd can accumulate in the chicken pancreas and affect the ion profiles, whereas Se can ameliorate the accumulation of Cd and change the ion profiles in the chicken pancreas.