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1.
A genetic linkage map of durum wheat 总被引:20,自引:6,他引:14
A. Blanco M. P. Bellomo A. Cenci C. De Giovanni R. D’Ovidio E. Iacono B. Laddomada M. A. Pagnotta E. Porceddu A. Sciancalepore R. Simeone O. A. Tanzarella 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):721-728
A genetic linkage map of tetraploid wheat [Triticum turgidum (L.) Thell.] was constructed using segregation data from a population of 65 recombinant inbred lines (RILs) derived from
a cross between the durum wheat cultivar Messapia and accession MG4343 of T. turgidum (L.) Thell. ssp dicoccoides (Korn.) Thell. A total of 259 loci were analysed, including 244 restriction fragment length polymorphisms (RFLPs), one PCR
(polymerase chain reaction) marker (a sequence coding for a LMW (low-molecular-weight) glutenin subunit gene located at the
Glu-B3 locus), seven biochemical (six seed-storage protein loci and one isozyme locus) and seven morphological markers. A total
of 213 loci were mapped at a LOD≥3 on all 14 chromosomes of the A and B genomes. The total length of the map is 1352 cM and
the average distance between adjacent markers is 6.3 cM. Forty six loci could not be mapped at a LOD≥3. A fraction (18.6%)
of the markers deviated significantly from the expected Mendelian ratios; clusters of loci showing distorted segregation were
found on chromosomes 1B, 3AL, 4AL, 6AL and 7AL. The durum wheat map was compared with the published maps of bread wheat using
several common RFLP markers and general features are discussed. The markers detected the known structural rearrangements involving
chromosomes 4A, 5A and 7B as well as the translocation between 2B-6B, but not the deletion on 2BS. This map provides a useful
tool for analysing and breeding economically important quantitative traits and for marker-assisted selection, as well as for
studies of genome organisation in small grain cereal species.
Received: 5 January 1998 / Accepted: 31 March 1998 相似文献
2.
C. Du G. E. Hart 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(5):645-653
Triticum turgidum L. var ‘durum’ cv ‘Langdon’-T. t. var ‘dicoccoides’ chromosome 6A and 6B recombinant substitution lines (RSLs) and a F2 population derived from a ‘Langdon’-T. t. var ‘dicoccoides’ disomic chromosome 6A substitution lineבLangdon’ cross were analyzed with the objective of markedly increasing
the number of markers assigned to and the resolution of previously constructed 6A and 6B linkage maps. Fifty-seven markers
were added to the 6A RSL-population map, which now consists of 73 markers that span 111 cM, and 40 markers were added to the
6B RSL-population map, which now consists of 56 markers that span 123 cM. With the exception of 2 6B loci, all of the loci
on the two RSL-population maps were ordered at a LOD score ≥3.0. Thirty-seven orthologous markers were mapped in the two chromosomes
and colinearity between them is strongly indicated. The 6A RSL-population map and the F2-population map are highly similar, indicating that the former population, which consists of 66 lines, can be reliably used
for mapping, as was previously demonstrated for the 6B RSL population. In the absence of selection and genetic drift, the
lines in a RSL population, except at loci in the substituted/recombined chromosome, should be near-isogenic. An unexpected
finding was that at least 26 and possibly 29 of the RFLPs detected in the RSL populations (18% of the markers analyzed) are
not located in the substituted/recombined chromosomes. Linkage analysis of the markers disclosed that at least 19 of them
are located in six or seven segments that span approximately 10 cM and 17 cM of the genetic lengths of 6B and 6A, respectively,
in the 6A and 6B RSL populations, respectively, a finding that suggests that 40 or more alien segments spanning 8–15% of the
genetic length of the 13 unsubstituted chromosomes are present in both of the RSL populations. Alien alleles are fixed in
many RSLs for most of the markers, in most cases at a frequency consistent with theoretical expectations. Highly distorted
segregation favoring the alien allele was detected for all of the markers in 2 of the segments, however. Nine of the markers
were among those mapped in the substituted/recombined chromosomes; the linkage data obtained for the other 10 was sufficient
to assign them to approximate map positions.
Received: 12 June 1997 / Accepted: 6 October 1997 相似文献
3.
Polymorphism, distribution, and segregation of AFLP markers in a doubled haploid rice population 总被引:18,自引:0,他引:18
M. Maheswaran P. K. Subudhi S. Nandi J. C. Xu A. Parco D. C. Yang N. Huang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(1):39-45
We exploited the newly developed amplified fragment length polymorphism (AFLP) technique to study the polymorphism, distribution
and inheritance of AFLP markers with a doubled haploid rice population derived from ‘IR64’/‘Azucena’. Using only 20 pairs
of primer combinations, we detected 945 AFLP bands of which 208 were polymorphic. All 208 AFLP markers were mapped and distributed
over all 12 chromosomes. When these were compared with RFLP markers already mapped in the population, we found the AFLP markers
to be highly polymorphic in rice and to follow Mendelian segregation. As linkage map of rice can be generated rapidly with
AFLP markers they will be very useful for marker-assisted backcrossing.
Received: 11 April 1996 / Accepted: 14 June 1996 相似文献
4.
Analysis of complex leaf and flower characters in Rhododendron using a molecular linkage map 总被引:10,自引:0,他引:10
F. Dunemann R. Kahnau I. Stange 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):1146-1155
A molecular linkage map of Rhododendron has been constructed by using a segregating population from an interspecific cross. Parent-specific maps based on 239 RAPD,
38 RFLP, and two microsatellite markers were aligned using markers heterozygous in both parents. The map of the male parent
‘Cunningham’s White’ comprised 182 DNA markers in 13 linkage groups corresponding to the basic chromosome number. In the female
parent ‘Rh 16’ 168 markers were located on 18 linkage groups. An assignment of putative homologous linkage groups was possible
for 11 groups of each parent. QTL analyses based on the non-parametric Kruskal-Wallis rank-sum test were performed for the
characters “leaf chlorosis” and “flower colour” scored as quantitative traits. For leaf chlorosis, two genomic regions bearing
QTLs with significant effects on the trait were identified on two linkage groups of the chlorosis-tolerant parent. RAPD marker
analysis of additional lime-stressed genotypes tested under altered environmental conditions verified the relationship between
marker allele frequencies and the expression of chlorosis. Highly significant QTL effects for flower colour were found on
two chromosomes indicating major genes located in these genome areas. The prospects for utilization of a linkage map in Rhododendron are discussed.
Received: 28 September 1998 / Accepted: 5 November 1998 相似文献
5.
A molecular genetic map of cassava (Manihot esculenta Crantz) 总被引:12,自引:0,他引:12
M. Fregene F. Angel R. Gomez F. Rodriguez P. Chavarriaga W. Roca J. Tohme M. Bonierbale 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(3):431-441
A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers
segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM 2177-2’, elite cassava cultivars from Nigeria and Colombia, respectively. The
map consists of 20 linkage groups spanning 931.6 cM or an estimated 60% of the cassava genome. Average marker density is 1
per 7.9 cM. Since the mapping population is an F1 cross between heterozygous parents, with unique alleles segregating from either parent, a second map was constructed from
the segregation of 107 RFLPs, 50 RAPDs, 1 microsatellite, and 1 isoenzyme marker from the male parent. Comparison of intervals
in the male-and female-derived maps, bounded by markers heterozygous in both parents, revealed significantly less meiotic
recombination in the gametes of the female than in the male parent. Six pairs of duplicated loci were detected by low-copy
genomic and cDNA sequences used as probes. Efforts are underway to saturate the cassava map with additional markers, to join
the male- and female-derived maps, and to elucidate genome organization in cassava.
Received: 5 July 1996/Accepted: 22 November 1996 相似文献
6.
Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers 总被引:41,自引:0,他引:41
T. Kojima T. Nagaoka K. Noda Y. Ogihara 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):37-45
The potential of PCR-based markers for construction of a genetic linkage map in Einkorn wheat was investigated. From a comparison
of polymorphisms between two Einkorn wheats, Triticum monococcum (Mn) and T. boeoticum (Bt), we obtained 49 polymorphic bands produced by 33 primers for inter-simple sequence repeat (ISSR) and 36 polymorphic
bands shown by 25 combinations of random amplified polymorphic DNA (RAPD) primers for mapping in 66 individuals in the F2 population. Although 44 ISSR fragments and 29 RAPD fragments statistically showed a 3 : 1 segregation ratio in the F2 population, only 9 markers each of the ISSR and RAPD bands were able to be mapped on the RFLP linkage map of Einkorn wheat.
ISSR markers were distributed throughout the chromosomes. The mapped positions of the ISSR markers seemed to be similar to
those obtained by the RFLP markers. On the other hand, 4 of the 9 RAPD markers could map the RFLP marker-poor region on the
short arm of 3Am, suggesting a potential to map novel regions containing repetitive sequences. Comparisons of the genetic linkage map of Einkorn
wheat to the linkage map and cytological map of common wheat revealed that the marker orders between the two maps of Einkorn
wheat and common wheat coincided except for 4A, which harbors chromosome rearrangements specific for polyploid wheats, indicating
a conservatism between the two genomes. Recombinations in Einkorn wheat chromosomes took place more frequently around the
centromere and less at the distal part of chromosomes in comparison to those in common wheat. Nevertheless, recombinations
even in Einkorn wheat chromosomes were strongly suppressed around the centromere. In fact, the markers located within 1 cM
of the centromere were located almost in the central part of the chromosome arm.
Received: 7 June 1997 / Accepted: 17 June 1997 相似文献
7.
Construction of a composite sorghum genome map and comparison with sugarcane, a related complex polyploid 总被引:11,自引:0,他引:11
P. Dufour M. Deu L. Grivet A. D’Hont F. Paulet A. Bouet C. Lanaud J. C. Glaszmann P. Hamon 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(3-4):409-418
A sorghum composite linkage map was constructed with two recombinant inbred line populations using heterologous probes already
mapped on maize and sugarcane. This map includes 199 loci revealed by 188 probes and distributed on 13 linkage groups. A comparison
based on 84 common probes was performed between the sorghum composite map and a map of a sugarcane (Saccharum spp.) cultivar being developed and presently comprising 10 tentative linkage groups. A straight synteny was observed for
2 pairs of linkage groups; in two cases, 1 sorghum linkage group corresponded to 2 or 3 sugarcane linkage groups, respectively;
in two cases 1 sugarcane link- age group corresponded to 2 separate sorghum linkage groups; for 2 sorghum linkage groups,
no complete correspondance was found in the sugarcane genome. In most cases loci appeared to be colinear between homoeologous
chromosomal segments in sorghum and sugarcane. These results are discussed in relation to published data on sorghum genomic
maps, with specific reference to the genetic organization of sugarcane cultivars, and they, illustrate how investigations
on relatively simple diploid genomes as sorghum will facilitate the mapping of related polyploid species such as sugarcane.
Received: 12 August 1996 / Accepted: 30 August 1996 相似文献
8.
H. Kuang T. Richardson S. Carson P. Wilcox B. Bongarten 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(5):697-703
A genetic map of Pinus radiata plus tree 850.55 was constructed using megagametophytes of S1 seeds. The map contained 19 linkage groups, with 168 RAPD and four microsatellite markers. The total map length was 1116.7 cM
(Kosambi’s function) and was estimated to cover 56% of the genome. Of the 172 markers, 59 (34%) were distorted from the expected
1 : 1 ratio in megagametophytes (P<0.05). We show that if the distortion is caused by a single viability gene or by sampling error, the estimate of recombination
frequency in megagametophytes of selfed seeds would not be affected.
Received: 20 April 1998 / Accepted: 13 July 1998 相似文献
9.
H. Buerstmayr M. Lemmens G. Fedak P. Ruckenbauer Communicated B. S. Gill 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(1):76-85
Fusarium head blight (FHB or scab) caused by Fusarium spp. is a widespread disease of cereals causing yield and quality losses and contaminating cereal products with mycotoxins. The
breeding of resistant varieties is the method of choice for controlling the disease. Unfortunately, the genetic basis of scab
resistance is still poorly understood. We present the results of a back-cross reciprocal monosomic analysis of FHB resistance
using the highly resistant Hungarian winter wheat line ‘U-136.1’ and the highly susceptible cultivar ‘Hobbit-sib’. Resistance
testing was performed in a field trial artificially inoculated with a Fusarium culmorum conidial suspension. Five hemizygous families containing ‘U-136.1’ chromosomes 6B, 5A, 6D, 1B, and 4B had a visually reduced
spread of infection compared to lines having the ‘Hobbit-sib’ chromosome. Chromosome 2B from ‘U-136.1’ had an increased spread
of infection. The critical chromosomes controlling seed weight were 6D, 3B, 5A, and 6B while those controlling deoxynivalenol
(DON) content were homoeologous groups 2 and 6, although the latter effects were not significant due to a high coefficient
of variation. Results from this and other studies show that chromosomes 6D, 6B, 5A, 4D, and 7A have frequently been associated
with scab resistance in a number of wheat cultivars. Research groups now attempting to map scab resistance in wheat using
markers should pay special attention to the above-mentioned chromosomes.
Received: 31 March 1998 / Accepted: 14 July 1998 相似文献
10.
M. Mohan P. V. Sathyanarayanan A. Kumar M. N. Srivastava S. Nair 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):777-782
A PCR-based marker (E20570) linked to the gene Gm4t, which confers resistance to a dipteran pest gall midge (Orseolia oryzae), has been mapped using the restriction fragment length polymorphism (RFLP) technique in rice. Gm4t is a dominant resistance gene. We initially failed to detect useful polymorphism for this marker in a F3 mapping population derived from a cross between two indica parents, ‘Abhaya’בShyamala’, with as many as 35 restriction enzymes. ‘Abhaya’ carries the resistance gene Gm4t and ‘Shyamala’ is susceptible to gall midge. Subsequently, E20570 was mapped using another mapping population represented by a F2 progeny from a cross between ‘Nipponbare’, a japonica variety, and ‘Kasalath’, an indica variety, in which the gene Gm4t was not known to be present. Gm4t mapped onto chromosome 8 between markers R1813 and S1633B. Our method, thus, presents an alternative way of mapping genes
which otherwise would be difficult to map because of a lack of polymorphism between closely related parents differing in desired
agronomic traits.
Received: 1 April 1997 / Accepted: 13 May 1997 相似文献
11.
T. M. Fulton J. C. Nelson S. D. Tanksley 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):895-902
Segregation of the Lycopersicon peruvianum genome was followed through three generations of backcrossing to the cultivated tomato L. esculentum cv ‘E6203’ using molecular markers. Thirteen BC1 plants were genotyped with 113 markers, 67 BC2 plants with 84 markers, and finally 241 BC3 plants were genotyped with 177 markers covering the entire genome and a BC3 map constructed. Several segments of the genome, including parts of chromosomes 3, 4, 6, and 10, quickly became fixed for
esculentum alleles, possibly due to sterility problems encountered in the BC1. Observed overall heterozygosity and chromosome segment lengths at each generation were very near the expected theoretical
values. Markers located near the top telomeric region of chromosome 9 showed segregation highly skewed towards the wild allele
through all generations, suggesting the presence of a gamete promoter gene. One markers, TG9, mapped to a new position on
chromosome 9, implying an intrachromosomal translocation event. Despite the great genetic distance between the two parents,
overall recombination was only 25% less than that observed in a previous tomato cross, indicating that L. peruvianum genes may be more readily introgressed into cultivated germplasm than originally believed.
Received: 9 April 1997 / Accepted : 20 May 1997 相似文献
12.
V. Laucou K. Haurogné N. Ellis C. Rameau 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):905-915
A genetic linkage map of Pisum sativum L. was constructed based primarily on RAPD markers that were carefully selected for their reproducibility and scored in a
population of 139 recombinant inbred lines (RILs). The mapping population was derived from a cross between a protein-rich
dry-seed cultivar ‘Térèse’ and an increased branching mutant (K586) obtained from the pea cultivar ‘Torsdag’. The map currently
comprises nine linkage groups with two groups comprising only 6 markers (n=7 in pea) and covers 1139 cM. This RAPD-based map has been aligned with the map based on the (JI281×JI399) RILs population
that currently includes 355 markers in seven linkage groups covering 1881 cM. The difference in map lengths is discussed.
For this alignment 7 RFLPs, 23 RAPD markers, the morphological marker le and the PCR marker corresponding to the gene Uni were used as common markers and scored in both populations.
Received: 13 March 1998 / Accepted: 29 April 1998 相似文献
13.
A linkage map of the pea (Pisum sativum L.) genome containing cloned sequences of known function and expressed sequence tags (ESTs) 总被引:3,自引:0,他引:3
B. J. Gilpin J. A. McCallum T. J. Frew G. M. Timmerman-Vaughan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(8):1289-1299
A linkage map of the pea (Pisum sativum L.) genome is presented which is based on F2 plants produced by crossing the marrowfat cultivar ‘Primo’ and the blue-pea breeding line ‘OSU442-15’. This linkage map consists
of 209 markers and covers 1330 cM (Kosambi units) and includes RFLP, RAPD and AFLP markers. By mapping a number of anchor
loci, the ‘Primo’בOSU442-15’ map has been related to other pea linkage maps. A feature of the map is the incorporation of
29 loci representing genes of known function, obtained from other laboratories. The map also contains RFLP loci detected using
sequence-characterized cDNA clones developed in our laboratory. The putative identities of 38 of these cDNA clones were assigned
by examining public-sequence databases for protein or nucleotide-sequence similarities. The conversion of sequence-characterized
pea cDNAs into PCR-amplifiable and polymorphic sequence-tagged sites (STSs) was investigated using 18 pairs of primers designed
for single-copy sequences. Eleven polymorphic STSs were developed.
Received: 18 June 1997 / Accepted: 11 August 1997 相似文献
14.
S. R. Larson K. A. Young A. Cook T. K. Blake V. Raboy 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):141-146
This study describes the inheritance and linkage map positions of two low phytic acid barley (Hordeum vulgare) mutations, lpa1-1 and lpa2-1, that dramatically reduce grain phytic acid content and increase inorganic seed phosphorus (P). Wide-cross, F2 mapping populations were constructed by mating six-rowed varieties, ‘Steptoe’ and/or ‘Morex’, with two-rowed ‘Harrington’lpa donor lines homozygous for either lpa1-1 or lpa2-1. The barley lpa1-1 mutation showed normal inheritance patterns, whereas a deficiency of homozygous lpa2-1/lpa2-1 F2 plants was observed. We identified a codominant, STS-PCR marker (aMSU21) that cosegregated with lpa1-1 in a population of 41 F2 plants. The aMSU21 marker was then mapped to a locus on barley chromosome 2H, using a North American Barley Genome Mapping
Project (NABGMP) doubled haploid population (‘Harrington’בMorex’). We determined that lpa2-1 is located within a recombination interval of approximately 30 cM between two AFLP markers that were subsequently mapped
to barley chromosome 7H by integration with the same NABGMP population. Recent comparative mapping studies indicate conserved
genetic map orders of several homologous molecular marker loci in maize and the Triticeae species that also show corresponding
linkage to the biochemically similar lpa2 mutations of maize and barley. This observation suggests that barley and maize lpa2 mutations may affect orthologous genes. No such evidence for correspondence of the phenotypically similar lpa1 mutations of barley and maize has been revealed.
Received: 22 September 1997 / Accepted: 2 December 1997 相似文献
15.
J. M. H. Kijas M. R. Thomas J. C. S. Fowler M. L. Roose 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(5):701-706
We report the successful assignment of the first seven microsatellite markers to the Citrus RFLP and isozyme map. A total of 14 microsatellite primer pairs were developed and tested for amplification and product-length
polymorphism within a population of plants previously used for linkage-map construction. In each case, the successfully assigned
microsatellite mapped to the termini of a different linkage group indicating a widespread distribution throughout the genome.
Analysis of allele segregation revealed that two of nine microsatellites displayed a significant deviation from expected ratios
(P>0.5). This was compared with other marker types within Citrus and a similar proportion of skewed loci was also found to be present. The analysis of two markers was complicated by the
non-amplification of an inherited null allele within the mapping population. The successful integration of microsatellites
into the genetic map of Citrus demonstrates the utility of this marker type for genetic analysis within wide intergeneric plant crosses.
Received: 16 September 1996 / Accepted: 18 October 1996 相似文献
16.
A genetic linkage map of cowpea (Vigna unguiculata) developed from a cross between two inbred, domesticated lines 总被引:1,自引:0,他引:1
C. M. Menéndez A. E. Hall P. Gepts 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(8):1210-1217
We have constructed a genetic linkage map within the cultivated gene pool of cowpea (2n=2x=22) from an F8 recombinant inbred population (94 individuals) derived from a cross between the inbreds IT84S-2049 and 524B. These breeding
lines, developed in Nigeria and California, show contrasting reactions against several pests and diseases and differ in several
morphological traits. Parental lines were screened with 332 random RAPD decamers, 74 RFLP probes (bean, cowpea and mung bean
genomic DNA clones), and 17 AFLP primer combinations. RAPD primers were twice as efficient as AFLP primers and RFLP probes
in detecting polymorphisms in this cross. The map consists of 181 loci, comprising 133 RAPDs, 19 RFLPs, 25 AFLPs, three morphological/classical
markers, and a biochemical marker (dehydrin). These markers identified 12 linkage groups spanning 972 cM with an average distance
of 6.4 cM between markers. Linkage groups ranged from 3 to 257 cM in length and included from 2 to 41 markers, respectively.
A gene for earliness was mapped on linkage group 2. Seed weight showed a significant association with a RAPD marker on linkage
group 5. This map should facilitate the identification of markers that “tag” genes for pest and disease resistance and other
traits in the cultivated gene pool of cowpea.
Received: 16 September 1996 / Accepted: 25 April 1997 相似文献
17.
R. Testolin W. G. Huang O. Lain R. Messina A. Vecchione G. Cipriani 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(1):30-36
A genetic map of kiwifruit (Actinidia spp.) was constructed using microsatellite and AFLP markers and the pseudo-testcross mapping strategy. (AC)n and (AG)n microsatellite repeats were first isolated from Actinidia chinensis (2n = 2x = 58) enriched genomic libraries and tested for segregation in the interspecific cross between the diploid distantly
related species A. chinensis and A. callosa. Some 105 microsatellite loci of the 251 initially tested segregated in the progeny in a 1:1 ratio as in a classical backcross,
or in a ratio which could mimic the backcross, and were mapped using 94 individuals. AFLP markers were then produced using
MseI and EcoRI restriction enzymes and 15 primer combinations. Nearly 10% of loci showed a distorted segregation at α = 0.05, and only 4% at α = 0.01, irrespectively to the marker class. Two linkage maps
were produced, one for each parent. The female map had 203 loci, of which 160 (71 SSR and 89 AFLP) constituted the framework map at a LOD score ≥ 2.0. The map was 1,758.5 cM(K) long, covering
46% of the estimated genome length. The male map had only 143 loci, of which 116 (28 SSR, 87 AFLP and the sex determinant)
constituted the framework map. The map length was only 1,104.1 cM(K), covering 34% of the estimate genome length. Only 35
SSR loci were mapped in the male parent because 18% of SSR loci that were characterised did not amplify in A. callosa, and 48% were homozygous. The choice of parents in the pseudo-testcross is critically discussed. The sex determinant was
mapped in A. callosa.
Received: 27 July 2000 / Accepted: 31 October 2000 相似文献
18.
A Genetic linkage map of Pinyon pine (Pinus edulis) based on amplified fragment length polymorphisms 总被引:1,自引:0,他引:1
S. E. Travis K. Ritland T. G. Whitham P. Keim 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):871-880
Amplified fragment length polymorphisms (AFLP) were used to rapidly generate a dense linkage map for pinyon pine (Pinus edulis). The map population consisted of 40 megagametophytes derived from one tree at Sunset Crater, Arizona. A total of 78 primer
combinations, each with three to five selective nucleotides, amplified 542 polymorphic markers. Of these, 33 markers showed
significant deviation from the expected Mendelian genotypic segregation ratio of 1 : 1, and 164 showed complete linkage with
another marker. This resulted in 338 unique markers mapping to 25 linkage groups, each of which ranged from 2 to 22 markers,
averaging 80 centiMorgans (cM) in size and covering 2,012 cM (2,200 cM with the inclusion of 25 cM for each of 7 unlinked
markers). Pairwise linkage values gave a genome size estimate of 2,390 cM, suggesting comprehensive coverage of the genome.
A search for subsets of primer combinations giving the best map coverage found 10 primer combinations which together marked
72% of the linkage map to within 10 cM; an additional 10 primer combinations increased this percentage to 85%. Our map represents
an initial step towards the identification of quantitative trait loci associated with pest resistance and water stress in
pinyons and will further allow us to examine introgression rates between P. edulis and P. californiarum.
Received: 14 October 1997 / Accepted: 29 April 1998 相似文献
19.
P. Masojc´ B. Mys´ków P. Milczarski 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(8):1273-1279
RFLP-based genetic map of rye, developed previously using a cross of lines DS2×RXL10 (F2 generation), was extended with 69 RAPD and 12 isozyme markers. The actual map contains 282 markers dispersed on all seven
chromosomes and spans a distance of 1,140 cM. The efficiency of mapping RAPD markers was close to ten loci per 100-screened
arbitrary primers. A strong selection of polymorphic, intensive and reproducible fragments was necessary to reveal individual
marker loci that could be assigned to rye chromosomes. Newly mapped markers cover a substantial part of the rye genome and
constitute a valuable tool suitable for map saturation, marker-aided selection and phenetic studies. A specific nomenclature
for the RAPD loci mapped on individual rye chromosomes, which could be helpful in managing of accumulating data, is proposed.
Received: 8 May 2000 / Accepted: 17 October 2000 相似文献
20.
Construction of an integrated pepper map using RFLP,SSR, CAPS,AFLP, WRKY,rRAMP, and BAC end sequences 总被引:2,自引:1,他引:1
Heung-Ryul Lee Ik-Hyun Bae Soung-Woo Park Hyoun-Joung Kim Woong-Ki Min Jung-Heon Han Ki-Taek Kim Byung-Dong Kim 《Molecules and cells》2009,27(1):21-37
Map-based cloning to find genes of interest, markerassisted selection (MAS), and marker-assisted breeding (MAB) all require
good genetic maps with high reproducible markers. For map construction as well as chromosome assignment, development of single
copy PCR-based markers and map integration process are necessary. In this study, the 132 markers (57 STS from BAC-end sequences,
13 STS from RFLP, and 62 SSR) were newly developed as single copy type PCR-based markers. They were used together with 1830
markers previously developed in our lab to construct an integrated map with the Joinmap 3.0 program. This integrated map contained
169 SSR, 354 RFLP, 23 STS from BAC-end sequences, 6 STS from RFLP, 152 AFLP, 51 WRKY, and 99 rRAMP markers on 12 chromosomes.
The integrated map contained four genetic maps of two interspecific (Capsicum annuum ‘TF68’ and C. chinense ‘Habanero’) and two intraspecific (C. annuum ‘CM334’ and C. annuum ‘Chilsungcho’) populations of peppers. This constructed integrated map consisted of 805 markers (map distance of 1858 cM)
in interspecific populations and 745 markers (map distance of 1892 cM) in intraspecific populations. The used pepper STS were
first developed from end sequences of BAC clones from Capsicum annuum ‘CM334’. This integrated map will provide useful information for construction of future pepper genetic maps and for assignment
of linkage groups to pepper chromosomes. 相似文献