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1.
John C. McNamara 《Journal of experimental marine biology and ecology》1980,46(2):219-229
The relationships between pigment granules and the prominent smooth endoplasmic reticulum in the chromatophores of the shrimp, Palaemon affinis Heilprin, were investigated by transmission electron microscopy. Different types of pigment granules within the chromatophores were found to exhibit a close structural continuity with the cisternal membranes. The membranes of membrane-bound pigment granules were seen to be continuous with those of the ER cisternae, while pigment granules lacking membranes appear to adhere to the external cisternal surfaces. The reticulum, which seems to form a network enmeshing the pigment granules, is proposed to be part of a continuum linking these granules with their translocating force. 相似文献
2.
THE EFFECT OF STRYCHNINE AND LIGHT ON PIGMENTATION IN BLEPHARISMA UNDULANS STEIN 总被引:2,自引:2,他引:0
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The effect of strychnine sulfate and light on pigmentation in the ciliate protozoan Blepharisma undulans has been determined. Upon exposure of cells to strychnine, the pigment granules become loosened from their surrounding membranes. Eventually these membranes break and the granules are simultaneously released from the cell. At the cell surface, a fusion occurs between adjacent membraneless granules with the incorporation of membrane fragments. This fusion of granules and membrane fragments results in the formation of a pigmented "capsule" around the organism. After elimination of the pigment, the granule membranes remaining in the cytoplasm fuse to form apparently empty vesicles. Other cell organelles are generally undisturbed. A similar situation occurs upon exposure of cells to artificial light for 12 to 18 hr, however, the slow elimination of granules from the cells under these conditions does not result in the formation of a pigmented "capsule." The possible mechanisms of these reactions are discussed. 相似文献
3.
John C. McNamara 《Journal of experimental marine biology and ecology》1979,40(2):193-199
The changes in cell shape accompanying pigment aggregation and dispersion in the hindgut chromatophores of the shrimp Palaemon affinis Heilprin were investigated by scanning electron microscopy. On the dorsal surface of the hindgut, chromatophores with dispersed pigment appear as flattened discs from which radiate short cell extensions. Chromatophores with aggregated pigment appear as raised hemispherical masses which lack cell extensions. These transformations, the result of the movements of pigment granules and cytoplasm, are discussed in relation to the degree of contact between the chromatophores and the surrounding tissues. 相似文献
4.
V I Rodionov A G Vardanyan V F Kamalov V I Gelfand 《Cell biology international reports》1987,11(8):565-572
The melanophores of the teleost Gymnocorymbus ternetzi are filled with pigment granules, melanosomes, which in response to appropriate treatments, can disperse throughout the cytoplasm or form an aggregate in the cell center. Melanophores with the dispersed pigment were irradiated by a laser microbeam, focused on the cell center by the microscope objective. If the average energy of the microbeam was 6-7 microJ, either the center of the melanophore was damaged and a single ring-shaped fragment was formed, or the cell was broken into several fragments of smaller size. The fragments retained their ability to move the pigment granules. In ring-shaped fragments, after adrenaline treatment, the melanosomes formed a ring-shaped aggregate moving away from both outer and inner (irradiation-produced) margins of the fragment. The smaller fragments treated with adrenaline moved the pigment to their centers. Both small and ring-shaped fragments could aggregate melanosomes as soon as 5 minutes after irradiation. 相似文献
5.
When the retinal pigment epithelial cells of chick embryo are cultured in monolayer conditions, the pigment granules are lost from the cytoplasm. The first structural change in depigmentation is the transformation of pigment granules into the degradative organelles designated as the dense body and melanosome complex. The cells are grown in medium containing DBcAMP of various doses from 10?5 to 10?2M. Cell proliferation is retarded by treatment with DBcAMP (10?3M). The transformation of pigment granules is almost completely prevented in all 1-day cultured cells. In 5-day cultured cells continuously treated with more than 10?4M, the transformation is not only prevented, but the synthesis of pigment granules is stimulated. A similar result is obtained by the administration of 10?3M theophylline. 5′-AMP does not prevent the transformation of pigment granules but seems to stimulate the synthesis of pigment granules. On the other hand, cGMP is ineffective both on prevention of transformation and on synthesis of pigment granules. The mechanisms of the transformation of pigment granules are discussed. 相似文献
6.
LOCALIZATION OF ACID PHOSPHATASE IN LIPOFUSCIN GRANULES AND POSSIBLE AUTOPHAGIC VACUOLES IN INTERSTITIAL CELLS OF THE GUINEA PIG TESTIS
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The intracellular localization of acid phosphatase in guinea pig testicular interstitial cells was investigated by incubating nonfrozen thick sections of glutaraldehyde-perfused testis in a modified Gomori medium and preparing the tissue for electron microscopy. Lipofuscin pigment granules in these cells contain dense pigment, granular matrix, and often a lipid droplet. Reaction product is seen in the matrix of the pigment granules, and they may therefore be called residual bodies. At least some of the dense pigment appears to be derived from myelin figures and membrane whorls, since suitable intermediates can be seen. Lipid droplets found free in the cytoplasm are another possible source of pigment. In both cases the chemical mechanism is presumed to be autoxidation of unsaturated lipid. Acid phosphatase is present in the inner cisterna of Golgi elements. Enzyme activity also appears in possible autophagic vacuoles bounded by double membranes; the reaction product lies between the membranes. Consideration of the enzyme as a tracer suggests that the autophagic vacuoles are derived from the Golgi complex. Possible stages in the formation of these vacuoles by the inner Golgi cisternae are observed. 相似文献
7.
A. -C. Järemo Jonson D. -E. Nilsson 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1994,174(6):701-706
The energy dependence of the pupil pigment-migrations in the fly Musca domestica was studied in live animals, using optical techniques and nitrogen-gas induced anoxia. The results obtained can be summarized in 3 points:
- Energy deficiency can make the pupil mechanism stop in any state, extreme or intermediate.
- Anoxia induced during intermittent stimulation makes the pupil stop in the closed state (aggregated pigment granules).
- During long-term anoxia the pupil very slowly opens (dispersal of pigment granules), irrespective of ambient intensity.
8.
Summary The general fine structure of intestinal cells and changes which occur in ultrastructure during development from fourth-stage to adult N. dubius are reported. In fourth-stage worms pigment granules are prominent in intestinal cells. In adults the number of pigment granules appears to be reduced and phagolysosomes containing membranous profiles and pigment material increase in number. Another reorganization of cell structure involves mitochondria which are randomly distributed in the cytoplasm of cells in fourth-stage worms, concentrated in the apical cytoplasm in worms in the molting process, and confined to the base of cells in adult worms. Other changes involved structure of the nucleus, rough endoplasmic reticulum and glycogen content of cells.This investigation was supported, in part, by NIH Fellowships I-FI-GM-32750 and 5-F02-AI-32750. 相似文献
9.
U Schraermeyer 《Comparative biochemistry and physiology. B, Comparative biochemistry》1992,103(2):435-442
1. The ultrastructure of the retinal pigment epithelium (RPE) of adult Syrian golden hamsters and cattle was examined with respect to pigment granules and phagosomes involved in degradation of disk membranes from rod outer segments. 2. In the RPE of cattle, phagosomes were found that contained an electron-dense melanin-like material that was not autofluorescent and therefore not lipofuscin. 3. Disk membranes of rods are about 4 nm thick and become enlarged (7-20 nm) and electron-dense during degradation in the RPE. 4. Additionally electron-dense vesiculo-globular bodies (10-100 nm) were found in phagosomes during disk membrane degradation and in mature melanin granules. 5. In the RPE of adult hamsters that had been exposed to intense light, premelanosomes containing unmelanised filaments with a striated periodicity were found in the cytoplasm or in association with mature melanin granules. Early and late stage melanosomes were also present. Phagosomes in the RPE contained degraded disk membranes, melanin-like material and melanofilaments. 6. Dopa oxidase was detected ultrastructurally within shed disk membranes that were in close contact with the microvilli of the RPE. 7. The possibility of melanogenesis within phagosomes during disk membrane degradation is discussed. 相似文献
10.
SYNOPSIS. The structural changes leading to the formation of motile microgametes from a single immobile intracellular gametocyte have been examiued in the electron microscope. After pigeon blood infected with Haemoproteus columbae was exposed to the air at room temperature for a few minutes axonemes appeared in the parasite's cytoplasm and the cytoplasm itself appeared less dense. The axonemes were connected with bundles of intranuclear microtubules that were perhaps spindle fibers. No conventional kinetosomes or centrioles have been observed. After the microgametocyte left the erythrocyte, it assumed the shape of a polarized slug or a dumb-bell. Half of the organism was surrounded by a single membrane and filled by part of the nucleus. The other half was surrounded by the remains of the multiple membranes of the gametocyte and contained pigment granules, mitochondria, axonemes and nuclear extensions. The axonemes and nuclear extensions were segregated at the periphery of the cell, exterior to the gametocyte's inner membrane, and were assembled in situ into microgametes. The mature microgamete appeared to peel off from the gametocyte, leaving a residual body. 相似文献
11.
Cream markings aligned along the dorsal region of the female isopod, A. vulgare, were investigated with light and a fluorescence microscope and an electron microscope. Biochemical studies were also carried out. The cream markings were observed in the dorsal integument as a group of cream-colored chromatophores that emit a yellow fluorescence. These chromatophores, which are distinguishable from ommochrome chromatophores, contained numerous granules in the cytoplasm, and these granules (0.6–3.0 μm in length by 0.4–1.5 μm in width) were electron-lucent and spheroidal in shape with a concentric arrangement of membranes. Based on various biochemical analyses, the principal component of the yellow pigment isolated from the cream markings was identified as sepiapterin. These facts revealed that the cream markings are the chromatophores that contain pteridine granules. The males have no cream markings like those of the females, since the cream-colored chromatophores are externally hidden by the ommochrome chromatophore layer. The content of sepiapterin in the males was about two times greater than that in the females. This quantitative difference in sepiapterin content between males and females suggests that the pteridine formation in this pigment cell may be regulated by hormones associated with sex determination. 相似文献
12.
Sub-cellular Localisation of the White/Scarlet ABC Transporter to Pigment Granule Membranes Within the Compound Eye of Drosophila Melanogaster 总被引:4,自引:0,他引:4
The white, scarlet, and browngenes of Drosophila melanogasterencode ABC transporters involved with the uptake and storage of metabolic precursors to the red and brown eye colour pigments.
It has generally been assumed that these proteins are localised in the plasma membrane and transport precursor molecules from
the heamolymph into the eye pigment cells. However, the immuno-electron microscopy experiments in this study reveal that the
White and Scarlet proteins are located in the membranes of pigment granules within pigment cells and retinula cells of the
compound eye. No evidence of their presence in the plasma membrane was observed. This result suggests that, rather than tranporting
tryptophan into the cell across the plasma membrane, the White/Scarlet complex transports a metabolic intermediate (such as
3-hydroxy kynurenine) from the cytoplasm into the pigment granules. Other functional implications of this new finding are
discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
13.
Erythrophores isolated from the scales of the marine teleost, Holocentrus ascensionis (Osbeck), are capable of rapidly aggregating or dispersing numerous red pigment granules within their cytoplasm by translocating them along radial paths delineated by bundles of radially oriented microtubules. Pigment translocation is accompanied by transformations in the morphology of the cytoplasmic matrix, or microtrabecular lattice (MTL), in which the pigment granules are suspended. It appears that the MTL as a whole contracts toward the cell center during aggregation, carrying the pigment granules inward along with it, and is restructured during dispersion, using the radial microtubules as guides. We examined the energy requirements of pigment migration and the accompanying MTL transformations. Cellular ATP was depleted using the specific metabolic inhibitors 2,4 dinitrophenol, NaCN and oligomycin. All three of these drugs, which inhibit oxidative phosphorylation by different mechanisms, prevent both pigment dispersion and MTL transformation to dispersed morphology, while aggregation is unaffected. Inhibitor-treated cells recover normal pigment movements and MTL morphology when inhibitor is washed out of the cells with fresh medium. Potential energy apparently is stored in the MTL by some ATP-dependent process during dispersion and is converted to kinetic energy during aggregation. The results of this study strengthen the hypothesis that the MTL, working in concert with the radial microtubules, is the vehicle for pigment translocation in the erythrophore system. 相似文献
14.
《Insect Biochemistry》1990,20(8):785-792
A new type of pigment granule was found in the epidermal cells of the quail mutant of the silkworm, Bombyx mori. Electron microscopic observation shows this granule to be dense and distinct from the translucent pteridine granule. After the granules were isolated by sucrose density gradient centrifugation, the pigment was extracted and identified as xanthommatin.Xanthommatin localizes in the pigment granules binding with a protein. By SDS-polyacrylamide gel electrophoresis, the molecular weight of the pigment protein was estimated to be 13 kDa. The pigment granules may have a role in the biosynthesis and accumulation of xanthommatin. 相似文献
15.
F. Fava D. Di Gioia C. Romagnoli L. Marchetti D. Mares 《Archives of microbiology》1993,160(5):350-357
A strain of Pseudomonas fluorescens was capable of co-metabolizing 3-chlorobenzoic acid with the production of a chlorinated catechol black pigment. A peroxidase and another enzymatic activity referred to as a polyphenol oxidase were found to be involved in the oxidation of 4-chlorocatechol to 4-chloro-1,2-benzoquinone, i.e. in the production of highly reactive substrates for pigment formation. Therefore, P. fluorescens cells were seen to take an active part not only in 3-chlorobenzoate mineralization but also in overall pigment production. pH was found to be a key parameter in the regulation of the activity of P. fluorescens oxidoreductive enzymes. Ultrastructural investigations showed that electron dense granules of pigment were distributed throughout the cytoplasm of Pseudomonas fluorescens cells grown in presence of 3-chlorobenzoate, as confirmed also by Thiéry cytochemical investigations.In these cells, an extensive contraction of the cytoplasm as well as a significant damage to the cell wall after two days of incubation, suggested that pigment production caused a premature death of the cells accompanied by the leakage of the cell content. Pigment production seemed to occur mostly in the cytoplasmic context where the electron dense material accumulates until it is released in the medium after the cell lysis.Abbreviations 3-CBA
3-chlorobenzoic acid
- BA
benzoic acid
- 4-CC
4-chlorocatechol
- 3-CC
3-chlorocatechol
- MBTH
3-methyl-2-benzothiazolinone hydrazone
-
l-DOPA
l-3,4-dihydroxyphenyl-alanine
- SPB
sodium phosphate buffer 相似文献
16.
S. NEGISHI Y. HASEGAWA G. MARTIN P. JUCHAULT Y. KATAKURA 《Pigment cell & melanoma research》1994,7(3):184-190
The morphological characteristics and ommochrome quantity in the integument of red, white, and wild type (black-grey) Armadillidium vulgare were studied. The red phenotype was found to possess two kinds of immature ommochrome pigment granules within its pigment cells, in addition to mature pigment granules. The immature granules seemed to contain uniformly distributed fibrilles, or to have an electron-dense central region surrounded by an electron-lucent outer edge. Since these immature pigment granules were typically observed to be distributed along with the mature ones, and were also more easily extractable than the wild type's, it is hypothesized that ommochrome granule maturation in the red phenotype may occur slowly due to a defect in the pigment granule internal process which combines pigments with matrix proteins. Regarding the white phenotype, although its pigment cells were undeveloped, several large-sized vesicles containing a small amount of electron-dense material appeared in the pigment cell cytoplasm. The wild and red type males of A. vulgare were found to have an ommochrome content twice as large as that of the corresponding females, with no ommochrome pigment being detected in the white phenotype. The genetic relationship between the white and red phenotypes was discussed using as a basis the observed pigment granule structure. 相似文献
17.
HISAKO KAWAKAMI 《The Journal of eukaryotic microbiology》1984,31(2):247-253
Stentor niger collected in the suburbs of Hiroshima contained in its cytoplasm several hundreds of endosymbiotic algae and innumerable brownish pigment granules. The body of the ciliate was dark due to a mixture of the green endosymbiotic algae and brown pigment granules. The algae belonged to the genus Chlorella; each was enclosed in a perialgal vacuole and dispersed uniformly in the host cytoplasm from the myoneme layer inward to the center of the ciliate. The cell wall and plasma membrane of the alga enclosed a nucleus, chloroplast, mitochondrion, Golgi complex, accumulation bodies, myelinated vesicles, and many ribosomes. The chloroplast occupied more than half of the volume of the alga and contained a conspicuous pyrenoid. Algal multiplication occurred by two successive divisions of an alga, leading to four autospores within a perialgal vacuole; the walls of the vacuole invaginated to separate the autospores each into its own vacuole. Three types of pigment granules were scattered uniformly throughout the cytoplasm of the ciliate. The ultrastructure of the membranellar region, somatic cortex, and macro- and micronucleus of the ciliate are also described. 相似文献
18.
K M Liu C C Chang 《Proceedings of the National Science Council, Republic of China. Part B, Life sciences》1986,10(2):79-85
The ultrastructure of lipofuscin granules in neuroglial cells of the optic nerve of the Formosan Rock-Monkey was investigated by electron microscopy. In the cytoplasm of astroglial cells, numerous irregular lipofuscin granules were characterized by the presence of large lipid droplets, small electron-dense pigment granules, and some lamellar structures. The lipofuscin granules of the oligodendroglial cells were composed largely of dense, coarse pigment granules, multilinear structures, and a few small lipid droplets. The lipofuscin granules in microglial cells were characterized by numerous lipid droplets in various sizes, small electron-dense pigment granules, and prominent lamellar structures. It was reported that the lipofuscin granules are wear-and-tear materials and products from the cells in lower functional activity. However, our observations suggest that the presence of lipofuscin granules in the neuroglial cells of the optic nerve is likely a characteristic product of active phagocytosis. 相似文献
19.
Masamichi Aikawa Peter K. Hepler Clay G. Huff Helmuth Sprinz 《The Journal of cell biology》1966,28(2):355-373
Electron microscope studies of the erythrocytic forms, including gametocytes and asexual schizonts, of the protozoa Plasmodium fallax, P. lophurae, and P. cathemerium, have revealed a "cytostome," a specialized organelle of the pellicular membrane which is active in the ingestion of host cell cytoplasm. In material fixed in glutaraldehyde and postfixed in OsO4, the cytostome appears in face view as a pore limited by two dense circular membranes and having an inside diameter of approximately 190 mµ. In cross-section, the cytostome is a cavity bounded on each side by two dense segments corresponding to the two dense circles observed in face view; its base consists of a single unit membrane. In the process of feeding, the cytostome cavity enlarges by expansion of its membrane, permitting a large quantity of red cell cytoplasm to come into contact with the cytostome wall. Subsequent digestion of erythrocyte cytoplasm occurs exclusively in food vacuoles which emanate from the cytostome invagination. As digestion progresses, the food vacuoles initially stain more densely and there is a marked build-up of hemozoin granules. In the final stage of digestion, a single membrane surrounds a cluster of residual pigment particles and very little of the original host cell cytoplasm remains. The cytostome in exoerythrocytic stages of P. fallax has been observed only in merozoites and does not seem to play the same role in the feeding mechanism. 相似文献
20.
Salinity and high temperature stresses adversely affect growthand development of rice plants. To investigate the responseof rice cells to these stresses, we have analysed short-termstress-induced subcellular alterations in undifferentiated leafcells of rice seedlings by transmission electron microscopy.Perturbations noted particularly with respect to plasma membrane,mitochondrial membranes, endoplasmic reticulum, polyribosomesand dictyosomes are highlighted. The subcellular changes evokedby both stresses after 4 h were lysis of the cytoplasm, accumulationof electron-dense granules in the cytoplasm, distension in theER membranes, enhanced association of ribosomes with the endoplasmicreticulum, reduction in the number of mitochondrial cristae,as well as disorganization of cell wall fibrillar material.Certain changes were found to be unique to either the salinityor high temperature stress. Plasmolysis and increased cytoplasmicvesiculation were seen only in response to salinity stress,while discontinuity in the plasma membrane with close associationof the osmiophilic granules were observed only in response tohigh temperature.Copyright 1997 Annals of Botany Company Electron dense granules; high temperature stress; leaf cells; Oryza sativaL.; rice; salinity; ultrastructure 相似文献