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1.
Hoarau JY Grivet L Offmann B Raboin LM Diorflar JP Payet J Hellmann M D'Hont A Glaszmann JC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,105(6-7):1027-1037
The genetics of current sugarcane cultivars ( Saccharum spp.) is outstandingly complex, due to a high ploidy level and an interspecific origin which leads to the presence of numerous chromosomes belonging to two ancestral genomes. In order to analyse the inheritance of quantitative traits, we have undertaken an extensive Quantitative Trait Allele (QTA) mapping study based on a population of 295 progenies derived from the selfing of cultivar R570, using about 1,000 AFLP markers scattered on about half of the genome. The population was evaluated in a replicated trial for four basic yield components, plant height, stalk number, stalk diameter and brix, in two successive crop-cycles. Forty putative QTAs were found for the four traits at P = 5 x 10(-3), of which five appeared in both years. Their individual size ranged between 3 and 7% of the whole variation. The stability across years was improved when limiting threshold stringency. All these results depict the presence in the genome of numerous QTAs, with little effects, fluctuating slightly across cycles, on the verge to being perceptible given the experimental resolution. Epistatic interactions were also explored and 41 independent di-genic interactions were found at P = (5 x 10(-3))(2). Altogether the putative genetic factors revealed here explain from 30 to 55% of the total phenotypic variance depending on the trait. The tentative assignment of some QTAs to the ancestral genomes showed a small majority of contributions as expected from the ancestral phenotypes. This is the first extensive QTL mapping study performed in cultivated sugarcane. 相似文献
2.
Towards a saturated sorghum map using RFLP and AFLP markers 总被引:17,自引:3,他引:17
K. Boivin M. Deu J.-F. Rami G. Trouche P. Hamon 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(2):320-328
A near-saturated sorghum genetic linkage map was produced using RFLP, AFLP and morphological markers. First a composite,
essentially RFLP-based genetic linkage map was obtained from analyses of two recombinant inbred populations. This map includes
343 loci for 11 linkage groups spanning 1352 cM. Since this map was constructed with many previously mapped heterologous probes,
it offers a good basis for synteny studies. Separately, an AFLP map was obtained from the analysis of 168 bands revealed from
12 primer pair combinations. It includes 137 loci for 11 linkage groups spanning 849 cM. Taking into account the different
data sets, we constructed a combined genetic linkage map including 443 loci spanning 1899 cM. Two main features are to be
noted: (1) the distribution of AFLPs along the genome is not uniform; (2) an important stretching of the former core map is
induced after adding the AFLPs.
Received: 10 May 1998 / Accepted: 13 July 1998 相似文献
3.
Evaluation of genetic variation in the daylily (Hemerocallis spp.) using AFLP markers 总被引:1,自引:0,他引:1
J. P. Tomkins T. C. Wood L. S. Barnes A. Westman R. A. Wing 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(4):489-496
The daylily (Hemerocallis spp.) is one of the most economically important ornamental plant species in commerce. Interestingly, it is also one of the
most heavily bred crops during the past 60 years. Since the American Hemerocallis Society began acting as the official registry
of daylily cultivars in 1947, more than 40 000 registrations have been processed. In order to determine the effects of intensive
breeding on cultivar development, and to study relationships among different species, genetic variation in the daylily was
estimated using AFLP markers. Nineteen primary genotypes (species and early cultivars) and 100 modern cultivars from different
time periods were evaluated using 152 unambiguous bands (average 79% polymorphism rate) derived from three AFLP primer combinations.
Overall, pairwise similarity estimates between entries ranged between 0.618 and 0.926 (average=0.800). When comparing cultivar
groups from different time periods (1940–1998), genetic similarity was initially increased, compared to the primary diploid
genotypes, remained constant from 1940 to 1980, and then steadily increased as breeding efforts intensified and hybridizers
began focusing on a limited tetraploid germplasm pool derived by colchicine conversion. Among modern (1991–1998) daylily cultivars,
genetic similarity has increased by approximately 10% compared to the primary genotypes. These data were also used to evaluate
recent taxonomic classifications among daylily species which, with a few minor exceptions, were generally supported by the
AFLP data.
Received: 15 March 2000 / Accepted: 13 June 2000 相似文献
4.
中国明对虾AFLP分子标记遗传连锁图谱的构建 总被引:26,自引:0,他引:26
以中国明对虾抗WSSV(白斑综合症病毒,WhiteSpotSyndromeVirus)选育群体第四代为母本,野生中国明对虾为父本,采用单对杂交方式产生F1代,F1代个体姊妹交产生F2代共42个个体为做图群体。62对AFLP选择性引物组合共产生529个分离位点,符合1∶1孟德尔分离类型位点共253个,3∶1孟德尔分离类型位点共276个。利用拟测交理论分别构建中国明对虾雌虾、雄虾的遗传连锁图谱,利用F2自交模型构建共同的AFLP分子标记连锁图谱。三张连锁图上分别有31、25和44个连锁群,图谱分辨率为分别为2.4cM、2.4cM和2.1cM。标记间隔距离分别为12.20cM、11.45cM和11.12cM图谱覆盖率分别达到50.21%、51.93%和48.08%。能够基本满足进行QTL(数量性状位点,QuantitativeTraitLocus)定位的需要。将该图谱和其他对虾类遗传连锁图谱进行了比较分析,探讨了利用相关分子标记将已有图谱进行整合的可能。 相似文献
5.
Application of AFLP, RAPD and ISSR markers to genetic mapping of European and Japanese larch 总被引:25,自引:0,他引:25
A. Arcade F. Anselin P. Faivre Rampant M. C. Lesage L. E. Pâques D. Prat 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(2):299-307
Genetic linkage maps have been increasingly developed for a wide variety of plants, using segregating populations such as
F2s or backcrosses between inbred lines. These pedigrees are rarely available in outbred species like forest trees which have
long generation times. Thus genetic mapping studies have to use peculiar pedigrees and markers in appropriate configurations.
We constructed single-tree genetic linkage maps of European larch (Larix decidua Mill.) and Japanese larch [Larix kaempferi (Lamb.) Carr.] using segregation data from 112 progeny individuals of an hybrid family. A total of 266 markers (114 AFLP,
149 RAPD and 3 ISSR loci) showing a testcross configuration, i.e.heterozygous in one parent and null in the other parent,
were grouped at LOD 4.0, θ=0.3. The maternal parent map (L. decidua)consisted of 117 markers partitioned within 17 linkage groups (1152 cM) and the paternal parent map (L. kaempferi) had 125 markers assembled into 21 linkage groups (1206 cM). The map distance covered by markers was determined by adding
a 34.7-cM independence distance at the end of each group and unlinked marker. It reached 2537 cM and 2997 cM respectively
for European larch and Japanese larch, and represented respectively a 79.6% and 80.8% coverage of the overall genome. A few
3:1 segregating markers were used to identify homologous linkage groups between the European larch and the Japanese larch
genetic maps. The PCR-based molecular markers allowed the construction of genetic maps, thus ensuring a good coverage of the
larch genome for further QTL detection and mapping studies.
Received: 15 March 1999 / Accepted: 29 March 1999 相似文献
6.
A. Singh M. S. Negi J. Rajagopal S. Bhatia U. K. Tomar P. S. Srivastava M. Lakshmikumaran 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):272-279
Genetic diversity was estimated in 37 neem accessions from different eco-geographic regions of India and four exotic lines
from Thailand using AFLP markers. Seven AFLP selective primer combinations generated a total of 422 amplification products.
The average number of scorable fragments was 60 per experiment, and a high degree (69.8%) of polymorphism was obtained per
assay with values ranging from 58% to 83.8%. Several rare and accession-specific bands were identified which could be effectively
used to distinguish the different genotypes. Genetic relationships within the accessions were evaluated by generating a similarity
matrix based on the Jaccard index. The phenetic dendrogram generated by UPGMA as well as principal correspondence analysis
separated the 37 Indian genotypes from the four Thai lines. The cluster analysis indicated that neem germplasm within India
constitutes a broad genetic base with the values of genetic similarity coefficient ranging from 0.74 to 0.93. Also, the Indian
genotypes were more dispersed on the principal correspondence plot, indicating a wide genetic base. The four lines from Thailand,
on the other hand, formed a narrow genetic base with similarity coefficients ranging from 0.88 to 0.92. The lowest genetic
similarity coefficient value (0.47) was observed between an Indian and an exotic genotype. The level of genetic variation
detected within the neem accessions with AFLP analysis suggests that it is an efficient marker technology for delineating
genetic relationships amongst genotypes and estimating genetic diversity, thereby enabling the formulation of appropriate
strategies for conservation and tree improvement programs.
Received: 20 October 1998 / Accepted: 28 November 1998 相似文献
7.
AFLP genetic maps of Eucalyptus globulus and E. tereticornis 总被引:8,自引:0,他引:8
C. M. Marques J. A. Araújo J. G. Ferreira R. Whetten D. M. O’Malley B.-H. Liu R. Sederoff 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(6-7):727-737
Amplified fragment length polymorphism (AFLP) analysis is a rapid and efficient technique for detecting large numbers of
DNA markers in eucalypts. We have used AFLP markers in a two-way pseudo-testcross strategy to generate genetic maps of two
clones of different Eucalyptus species (E. tereticornis and E. globulus). Of 606 polymorphic fragments scored, 487 segregated in a 1 : 1 ratio, corresponding to DNA polymorphisms heterozygous in
one parent and null in the other. In the maternal E. tereticornis map, 268 markers were ordered in 14 linkage groups (919 cM); the paternal E. globulus map had 200 markers in 16 linkage groups (967 cM). Results from PGRI software were compared with MAPMAKER. The average density
of markers was approximately 1 per 3.9 cM. Framework markers were ordered with an average confidence level of 90%, covering
80–100% of the estimated Eucalyptus genome size. In order to investigate the homologies between the E. tereticornis and the E. globulus genetic linkage maps, we included 19 markers segregating 3 : 1 in the analysis. Some homeologous linkage groups were recognized.
The linkage data developed in these maps will be used to detect loci controlling commercially important traits.
Received: 17 July 1997 / Accepted: 13 October 1997 相似文献
8.
Use of locus-specific AFLP markers to construct a high-density molecular map in barley 总被引:33,自引:5,他引:28
X. Qi P. Stam P. Lindhout 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(3-4):376-384
By using 25 primer combinations, 563 AFLP markers segregating in a recombinant inbred population (103 lines, F9) derived from L94/Vada were generated. The 38 AFLP markers in common to the existing AFLP/RFLP combined Proctor/Nudinka map,
one STS marker, and four phenotypic markers with known map positions, were used to assign present AFLP linkage groups to barley
chromosomes. The constructed high-density molecular map contains 561 AFLP markers, three morphological markers, one disease
resistance gene and one STS marker, and covers a 1062-cM genetic distance, corresponding to an average of one marker per 1.9
cM. However, extremely uneven distributions of AFLP markers and strong clustering of markers around the centromere were identified
in the present AFLP map. Around the centromeric region, 289 markers cover a genetic distance of 155 cM, corresponding to one
marker per 0.5 cM; on the distal parts, 906 cM were covered by 277 markers, corresponding to one marker per 3.3 cM. Three
gaps larger than 20 cM still exist on chromosomes 1, 3 and 5. A skeletal map with a uniform distribution of markers can be
extracted from the high-density map, and can be applied to detect and map loci underlying quantitative traits. However, the
application of this map is restricted to barley species since hardly any marker in common to a closely related Triticum species could be identified.
Received: 16 June 1997 / Accepted: 9 October 1997 相似文献
9.
Analysis of genetic similarity detected by AFLP and coefficient of parentage among genotypes of sugar cane ( Saccharum spp.) 总被引:7,自引:0,他引:7
Lima ML Garcia AA Oliveira KM Matsuoka S Arizono H De Souza CL De Souza AP 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(1):30-38
Despite the economical importance of sugar cane, until the present-date no studies have been carried out to determine the
correlation of the molecular-based genetic similarity (GS) and the coefficient of parentage (f)-estimates generated for cultivars. A comprehensive knowledge of the amount of genetic diversity in parental cultivars, could
improve the effectiveness of breeding programmes. In this study, amplified fragment length polymorphism (AFLP) and pedigree
data were used to investigate the genetic relationship in a group of 79 cultivars (interspecific hybrids), used as parents
in one of the Brazilian breeding programmes, and four species of Saccharum (Saccharum sinense, Saccharum barberi and two of Saccharum officinarum) . The objectives of this study were to assess the level of genetic similarity among the sugar-cane cultivars and to investigate
the correlation between the AFLP-based GS and f, based on pedigree information. Twenty one primer combinations were used to obtain the AFLP molecular markers, generating
a total of 2,331 bands, of which 1,121 were polymorphic, with a polymorphism rate, on average, of 50% per primer combination.
GSs were determined using Jaccard’s similarity coefficient, and a final dendrogram was constructed using an unweighted pair-group
method using arithmetic average (UPGMA). AFLP-based GS ranged from 0.28 to 0.89, with a mean of 0.47, whereas f ranged from 0 to 0.503, with a mean of 0.057. Cluster analysis using GS divided the genotypes into related subgroups suggesting
that there is important genetic relationship among the cultivars. AFLP-based GS and f were significantly correlated (r = 0.42, P < 0.001), thus the significance of this r value suggests that the AFLP data may help to more-accurately quantify the degree of relationship among sugar-cane cultivars.
Received: 27 November 2000 / Accepted: 27 April 2001 相似文献
10.
P. F. Bert G. Charmet P. Sourdille M. D. Hayward F. Balfourier 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(3-4):445-452
AFLP markers have been successfully employed for the development of a high-density linkage map of ryegrass (Lolium
perenne L.) using a progeny set of 95 plants from a testcross involving a doubled-haploid tester. This genetic map covered 930 cM
in seven linkage groups and was based on 463 amplified fragment length polymorphism (AFLP) markers using 17 primer pairs,
three isozymes and five EST markers. The average density of markers was approximately 1 per 2.0 cM. However, strong clustering
of AFLP markers was observed at putative centromeric regions. Around these regions, 272 markers covered about 137 cM whereas
the remaining 199 markers covered approximately 793 cM. Most genetic distances between consecutive pairs of markers were smaller
than 20 cM except for five gaps on groups A, C, D, F and G. A skeletal map with a uniform distribution of markers can be extracted
from this high-density map, and can be applied to detect and map QTLs. We report here the application of AFLP markers to genome
mapping, in Lolium as a prelude to quantitative trait locus (QTL) identification for diverse agronomic traits in ryegrass and for marker-assisted
plant breeding.
Received: 4 November 1998 / Accepted:15 March 1999 相似文献
11.
H. Kuang T. Richardson S. Carson P. Wilcox B. Bongarten 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(5):697-703
A genetic map of Pinus radiata plus tree 850.55 was constructed using megagametophytes of S1 seeds. The map contained 19 linkage groups, with 168 RAPD and four microsatellite markers. The total map length was 1116.7 cM
(Kosambi’s function) and was estimated to cover 56% of the genome. Of the 172 markers, 59 (34%) were distorted from the expected
1 : 1 ratio in megagametophytes (P<0.05). We show that if the distortion is caused by a single viability gene or by sampling error, the estimate of recombination
frequency in megagametophytes of selfed seeds would not be affected.
Received: 20 April 1998 / Accepted: 13 July 1998 相似文献
12.
D. Lespinasse M. Rodier-Goud L. Grivet A. Leconte H. Legnate M. Seguin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(1):127-138
The first genetic map for Hevea spp. (2n=36) is presented here. It is based on a F1 progeny of 106 individuals allowing the construction of a female, a male, and a synthetic map according to the pseudo-testcross
strategy. Progeny were derived from an interspecific cross between PB260, a H. brasiliensis cultivated clone, and RO38, a H. brasiliensis×H. benthamiana interspecific hybrid clone. The disomic inheritance observed for all the codominant markers scattered on the 2n=36 chromosomes
revealed that Hevea behaves as diploids. Homologous linkage groups between the two parental maps were merged using bridge loci. A total of 717
loci constituted the synthetic map, including 301 RFLPs, 388 AFLPs, 18 microsatellites, and 10 isozymes. The markers were
assembled into 18 linkage groups, thus reflecting the basic chromosome number, and covered a total distance of 2144 cM. Nine
markers were found to be unlinked. Segregation distortion was rare (1.4%). Average marker density was 1 per 3 cM. Comparison
of the distance between loci in the parental maps revealed significantly less meiotic recombination in the interspecific hybrid
male parent than in the female parent. Hevea origin and genome organisation are discussed.
Received: 2 February 1999 / Accepted: 11 March 1999 相似文献
13.
Assessment of genetic diversity in a Morus germplasm collection using fluorescence-based AFLP markers 总被引:6,自引:0,他引:6
A. Sharma R. Sharma H. Machii 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(7):1049-1055
To meet various breeding objectives and to conserve the existing genetic resources of mulberry for future use, the present
study was undertaken to investigate the amount of genetic diversity and to establish the relationships between mulberry genotypes
using fluorescence-based AFLP markers. Genetic diversity was estimated in 45 mulberry accessions from different eco-geographic
regions of Japan and other parts of the world. Five primer combinations amplified an average of 110 AFLP markers per primer
combination, ranging in size from 35 to 500 bp. A high degree of polymorphism was revealed by these combinations that ranged
from 69.7 to 82.3% across all the genotypes studied. Several rare genotype-specific bands were also identified which could
be effectively utilized to distinguish different genotypes. The wide range in genetic similarity coefficients (0.58–0.99)
indicated that the mulberry germplasm collection represents a genetically diverse popu-lation. The phenetic dendrogram generated
by the UPGMA method grouped 45 accessions into four major clusters, which was in agreement with the results from conventional
methods. Clustering of some genotypes into strictly separate groups was not readily apparent and no clear interrelationships
could be depicted, in spite of their different geographic origin. In addition, AFLP analysis provided sufficient polymorphism
for DNA typing and contributed additional insights into the genetic structure of the mulberry germplasm. These results will
help in the formulation of appropriate strategies for conservation and variety improvement in mulberry, for which little or
no knowledge of genetic diversity is currently available.
Received: 30 December 1999 / Accepted: 14 March 2000 相似文献
14.
R. Testolin W. G. Huang O. Lain R. Messina A. Vecchione G. Cipriani 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(1):30-36
A genetic map of kiwifruit (Actinidia spp.) was constructed using microsatellite and AFLP markers and the pseudo-testcross mapping strategy. (AC)n and (AG)n microsatellite repeats were first isolated from Actinidia chinensis (2n = 2x = 58) enriched genomic libraries and tested for segregation in the interspecific cross between the diploid distantly
related species A. chinensis and A. callosa. Some 105 microsatellite loci of the 251 initially tested segregated in the progeny in a 1:1 ratio as in a classical backcross,
or in a ratio which could mimic the backcross, and were mapped using 94 individuals. AFLP markers were then produced using
MseI and EcoRI restriction enzymes and 15 primer combinations. Nearly 10% of loci showed a distorted segregation at α = 0.05, and only 4% at α = 0.01, irrespectively to the marker class. Two linkage maps
were produced, one for each parent. The female map had 203 loci, of which 160 (71 SSR and 89 AFLP) constituted the framework map at a LOD score ≥ 2.0. The map was 1,758.5 cM(K) long, covering
46% of the estimated genome length. The male map had only 143 loci, of which 116 (28 SSR, 87 AFLP and the sex determinant)
constituted the framework map. The map length was only 1,104.1 cM(K), covering 34% of the estimate genome length. Only 35
SSR loci were mapped in the male parent because 18% of SSR loci that were characterised did not amplify in A. callosa, and 48% were homozygous. The choice of parents in the pseudo-testcross is critically discussed. The sex determinant was
mapped in A. callosa.
Received: 27 July 2000 / Accepted: 31 October 2000 相似文献
15.
M. L. Xu S. S. Korban 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(5-6):844-851
Using the amplified fragment length polymorphism (AFLP) technique combined with a ”narrow-down” bulk segregant strategy enabled
us to quickly identify 15 tightly linked AFLP markers to the Vf gene that confers resistance to the apple scab disease. High-resolution mapping placed all 15 AFLP markers within an interval
of 0.6 cM around the Vf region; 7 of them were found to be inseparable from the Vf gene, 1 was localized left of, and the remaining 7 located right of the Vf gene. In addition, eight previously identified RAPD markers were also mapped, but only three, including M18, AM19, and AL07,
were localized within this short interval, and none co-segregated with the Vf gene. The saturation of the Vf region with AFLP markers will accelerate both marker-assisted selection and map-based cloning. The advantages of this ”narrow-down”
strategy, estimation of physical distances among AFLP markers, and their potential application are also discussed.
Received: 22 December 1999 / Accepted: 25 March 2000 相似文献
16.
I. Eujayl M. Baum W. Powell W. Erskine E. Pehu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):83-89
A genetic linkage map of Lens sp. was constructed with 177 markers (89 RAPD, 79 AFLP, six RFLP and three morphological markers) using 86 recombinant inbred
lines (F6:8) obtained from a partially interspecific cross. The map covered 1073 cM of the lentil genome with an average distance of
6.0 cM between adjacent markers. Previously mapped RFLP markers were used as anchor probes. The morphological markers, pod
indehiscence, seed-coat pattern and flower-color loci were mapped. Out of the total linked loci, 8.4% showed segregation distortion.
More than one-fourth of the distorted loci were clustered in one linkage group. AFLP markers showed more segregation distortion
than the RAPD markers. The AFLP and RAPD markers were intermingled and clustering of AFLPs was seldom observed. This is the
most extensive genetic linkage map of lentil to-date. The marker density of this map could be used for the identification
of markers linked to quantitative trait loci in this population.
Received: 6 November 1997 / Accepted: 10 February 1998 相似文献
17.
AFLP markers in a molecular linkage map of maize: codominant scoring and linkage group ditsribution 总被引:1,自引:0,他引:1
P. Castiglioni P. Ajmone-Marsan R. van Wijk M. Motto 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(3-4):425-431
We exploited the AFLP technique to saturate a RFLP linkage map derived from a maize mapping population. By using two restriction
enzyme, EcoRI and PstI, differing in methylation sensitivity, both in combination with MseI, we detected 1568 bands of which 340 where polymorphic. These were added to the exitsing RFLP marker data to study the effects
of incorporation of AFLPs produced by different restriction-enzyme combinations upon genetic maps. Addition of the AFLP data
resulted in greater genome coverage, both through linking previously separate groups and the extension of other groups. The
increase of the total map length was mainly caused by the addition of markers to telomeric regions, where RFLP markers were
poorly represented. The percentage of informative loci was significantly different between the EcoRI and PstI assays. There was also evidence that PstI AFLP markers were more randomly distributed across chromosomes and chromosome regions, while EcoRI AFLP markers clustered mainly at centomeric regions. The more-random ditsribution of PstI AFLP markers on the genetic map reported here may reflect a preferential localisation of the markers in the hypomethylated
telomeric regions of the chromosomes.
Received: 22 December 1998 / Accepted: 25 March 1999 相似文献
18.
J. Garcia-Mas M. Oliver H. Gómez-Paniagua M.C. de Vicente 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(5-6):860-864
Three different types of molecular markers, RAPD, AFLP and RFLP were used to measure genetic diversity among six genotypes
of Cucumis melo L. Each line represented a different melon genotype: Piel de Sapo, Ogen, PI161375, PI414723, Agrestis and C105. A number
of polymorphic RAPD, AFLP and RFLP bands were scored on all materials and the genetic similarity measured. Clustering analysis
performed with the three types of markers separated the genotypes into two main groups: (1) the sweet type, cultivated melons
and (2) the exotic type, not cultivated melons. While the data obtained suggest that all three types of markers are equally
informative, AFLPs showed the highest efficiency in detecting polymorphism.
Received: 30 December 1999 / Accepted: 24 January 2000 相似文献
19.
Construction of a composite sorghum genome map and comparison with sugarcane, a related complex polyploid 总被引:11,自引:0,他引:11
P. Dufour M. Deu L. Grivet A. D’Hont F. Paulet A. Bouet C. Lanaud J. C. Glaszmann P. Hamon 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(3-4):409-418
A sorghum composite linkage map was constructed with two recombinant inbred line populations using heterologous probes already
mapped on maize and sugarcane. This map includes 199 loci revealed by 188 probes and distributed on 13 linkage groups. A comparison
based on 84 common probes was performed between the sorghum composite map and a map of a sugarcane (Saccharum spp.) cultivar being developed and presently comprising 10 tentative linkage groups. A straight synteny was observed for
2 pairs of linkage groups; in two cases, 1 sorghum linkage group corresponded to 2 or 3 sugarcane linkage groups, respectively;
in two cases 1 sugarcane link- age group corresponded to 2 separate sorghum linkage groups; for 2 sorghum linkage groups,
no complete correspondance was found in the sugarcane genome. In most cases loci appeared to be colinear between homoeologous
chromosomal segments in sorghum and sugarcane. These results are discussed in relation to published data on sorghum genomic
maps, with specific reference to the genetic organization of sugarcane cultivars, and they, illustrate how investigations
on relatively simple diploid genomes as sorghum will facilitate the mapping of related polyploid species such as sugarcane.
Received: 12 August 1996 / Accepted: 30 August 1996 相似文献
20.
Development of AFLP markers in barley 总被引:36,自引:0,他引:36
To investigate the application of amplified fragment length polymorphism (AFLP) markers in barley, 96 primer combinations
were used to generate AFLP patterns with two barley lines, L94 and Vada. With seven primer combinations, only a few intense
bands were obtained, probably derived from repeated sequences. With the majority of the remaining 89 primer combinations,
on average about 120 amplification products were generated, and the polymorphism rate between the two lines was generally
over 18%. Based on the number of amplified products and the polymorphism rate, the 48 best primer combinations were selected
and tested on 16 barley lines, again including L94 and Vada. Using a subset of 24 primer combinations 2188 clearly visible
bands within the range from 80 to 510 bp were generated; 55% of these showed some degree of polymorphism among the 16 lines.
L94 versus Vada showed the highest polymorphism rate (29%) and Proctor versus Nudinka yielded the lowest (12%). The polymorphism
rates per primer combination showed little dependence on the barley lines used. Hence the most efficient and informative primer
combinations identified for a given pair of lines turned out to be highly efficient when applied to others. Generally, more
than 100 common markers (possibly locus specific) among populations or crosses were easily identified by comparing 48 AFLP
profiles of the parent lines. The existence of such a large number of markers common to populations will facilitate the merging
of molecular marker data and other genetic data into one integrated genetic map of barley.
Received: 28 October 1996 / Accepted: 27 November 1996 相似文献