共查询到19条相似文献,搜索用时 93 毫秒
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SN_5对月季切花保鲜的作用 总被引:1,自引:0,他引:1
月季花朵硕大 ,色彩丰富 ,是当今国际市场上四大切花之一。然而关于月季切花保鲜的问题仍有待解决。虽然兼有强烈杀菌效果的乙烯作用抑制剂硫代硫酸银 ( STS)已经广泛作为月季切花保鲜剂的主要成分 ,但却存在延缓开花衰老效果不稳定和环境污染问题〔1,2〕。最近有一种以 PPOH(顺式丙烯基膦酸 )为主要成分的月季切花开花延缓剂已投放市场〔3,4〕。虽然 PPOH毒性较低 ,但作为乙烯结构类似物质 ,PPOH是以抑制乙烯自我催化为基本原理的保鲜药剂。黄绵佳等 〔5〕报道 PPOH降低了月季切花盛开前的花径增大率。可见 ,PPOH延缓了月季切花… 相似文献
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不同制剂在月季切花保鲜中的效果研究 总被引:3,自引:0,他引:3
以月季为材料,通过各种微生物保鲜剂的保鲜,了解其保鲜效果,并观察衰老过程中微生物与植物材料自身的生理变化,以探讨衰老原因。 相似文献
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保鲜剂对香石竹切花的保鲜效应 总被引:1,自引:0,他引:1
研究了保鲜剂(4%蔗糖 200 mg/L8-HQ 50 mg/L6-BA及4%蔗糖 0.1%明矾 0.02%尿素 0.02%NaCl)对香石竹切花的保鲜效应。结果表明,保鲜剂4%蔗糖 200 mg/L8-HQ 50 mg/L6-BA能明显地缓解切花水分胁迫,改善体内水分平衡,延缓切花衰老,延长切花的寿命。 相似文献
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表油菜素内酯对月季切花保鲜作用的研究 总被引:12,自引:1,他引:11
本文初步探讨了表油菜素内酯(epiBR)对瓶插月季切花的保鲜作用。与对照(蒸馏水)和基本液(2%蔗糖+500mgL-1柠檬酸+250mgL-8-羟基喹啉+25mgAgNO3)相比,经epiBR处理(基本液+0.1mgL-1epiBR)的月季切花花枝坚挺,蓝变延迟,瓶插寿命延长1-1.5倍。测定有关生理指标表明,epiBR处理对月季切花瓶插花枝前期鲜重的增加及后期的保持有明显作用。并显著延缓花瓣和叶片质膜相对透性的增加,还能使瓶插前期花瓣还原糖含量增加。epiBR处理对花瓣蛋白质和叶片叶绿素含量变化无明显影响,而对花瓣花青素水平下降有轻微的促进作用。 相似文献
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长安麦饭石在月季切花上的保鲜效果 总被引:4,自引:0,他引:4
在40g/L蔗糖+50mg/L SA 100mg/L Vc的保鲜液中,加入37μS/cm的长安麦饭石(CHMS),能更好的保持月季切花较高的吸水能力,增强切花花朵膜损伤修复能力,增大花朵开放直径,增加盛开前花枝的鲜重,提高切花瓶插期的观赏品质,延长瓶插寿命。本试验也证明了水杨酸(SA)与8-羟基喹啉(8-HQ)对月季切花有相似的保鲜效果。 相似文献
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巨大芽孢杆菌青霉素G酰化酶的定点突变及其动力学性质研究 总被引:3,自引:0,他引:3
为了提高青霉素G酰化酶(PGA)在酸性及有机溶剂中的稳定性,以大肠杆菌的晶体结构为模板,用软件PMODELING同源模建巨大芽孢杆菌青霉素G酰化酶的三维结构结构并且选择PGA分子表面的合适碱性氨基酸突变为丙氨酸,通过三种不同的快速PCR介导定位突变的方法,将位于PGA的α亚基21位、128位和β亚基492位、512位的赖氨酸残基分别突变为丙氨酸,获得四个突变酶Kα021A、Kα128A、Kβ492A和Kβ512A。其中Kα128A和Kβ512A保持与野生型相近的酶活力,其动力学性质如最适温度、最适pH,Km及Kcat没有明显变化;突变酶Kα021A和Kβ492A则丧失 了酶活力。上述结果表明,PGA分子表面非活性中心的赖氨酸→丙氨酸点突变使突变子的性状发生了分化,突变效应呈现出丰富的多样性。该有理设计不但可以提高酶的稳定性,而且为揭示PGA结构和功能的关系提供了一个新的研究模型。 相似文献
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Effect of broad-band ultraviolet and visible radiation on hydrogen peroxide formation by cultured rose cells 总被引:1,自引:0,他引:1
Terence M. Murphy 《Physiologia plantarum》1990,80(1):63-68
Broad-band radiation from a high-pressure Hg-vapor lamp, including ultraviolet wavelengths from 290 to 400 nm, blue, green and red wavelengths, did not induce the synthesis of H2 O2 in cultured rose cells. This was in contrast to the effects of shortwave (254 nm) ultraviolet radiation, even though, like shortwave ultraviolet radiation, the UV-B component of the broadband radiation induced a striking K+ efflux from the cells, and this efflux has been associated with H2 O2 synthesis in a previous report. The UV-A and visible wavelengths were shown to inhibit the synthesis of H2 O2 . This effect was associated with inhibition of peroxidase, an enzyme reported to be involved in the synthesis of H2 O2 in cell walls. UV-B radiation inhibited the alternate pathway for mitochondrial electron transport, but there was no evidence that this effect contributed to the inhibition of H2 O2 synthesis in cells treated with broad-band radiation. 相似文献
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Chengpeng Wang Yang Li Na Wang Qin Yu Yonghong Li Junping Gao Xiaofeng Zhou Nan Ma 《植物学报(英文版)》2023,65(4):895-899
The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-related nuclease 9(Cas9) system enables precise, simple editing of genes in many animals and plants.However, this system has not been applied to rose(Rosa hybrida) due to the genomic complexity and lack of an efficient transformation technology for this plant. Here, we established a platform for screening single-guide RNAs(sgRNAs) with high editing efficiency for CRISPR/Cas9-mediated gene editing in rose using suspensio... 相似文献
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含B9的预处理液对铁炮百合切花衰老的影响 总被引:6,自引:0,他引:6
预处理液[4%蔗糖 300mg·L-18-羟基喹啉柠檬酸盐 60 mg·L-1Al2(SO4)3 25 mg·L-1B9]能延长百合切花瓶插寿命3 d,增加鲜重,增加超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性,延缓丙二醛(MDA)含量的增加和超氧阴离子自由基(O·-2)产率,减少水分散失,维持膜结构的相对稳定性. 相似文献
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月季切花衰老过程中多胺与膜脂过氧化的关系 总被引:7,自引:0,他引:7
以月季切花为材料,研究了月季切花瓶插过程中多胺含量的变化,外源多胺处理对月季药花体内多胺含量的影响以及多胺与膜脂过氧化的关系。结果表明,月季切花瓶插衰老过程中腐胺在前2d略有增加,亚精胺和精胺均呈下降趋势;外源亚精胺和精胺处理均能增加切花体内多胺含量,并能延缓切花衰老和改善切花品质;且亚精胺和精胺处理降低了MDA含量的积累和膜相对透性的上升趋势。 相似文献
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Hydrolysis of penicillin G by combination of immobilized penicillin acylase and electrodialysis 总被引:1,自引:0,他引:1
Phenylacetic acid, as inhibitory product, was formed from a hydrolysis of penicillin G by immobilized penicillin acylase. In this article, electrodialysis was applied to remove phenylacetic acid continuously from the reaction mixture and to enhance an efficiency of the reaction. When 268 and 537 mM of penicillin G solution were used as the substrate, the concentration of phenylacetic acid in the reaction mixture could be maintained at less than 81 and 126 mM, respectively, and eventually, 86% and 88% of phenylacetic acid produced were removed from the reaction mixture at the end of the hydrolysis, respectively. Times required to reach 96% and 94.8% conversion from 268 and 537 mM of initial penicillin G could be reduced to 65% and 64% respectively, by means of electrodialysis; while 3.0% and 4.3% of initial penicillin G of 268 and 537 mM were permeated out of the reaction chamber during the hydrolysis, respectively. However, a loss of penicillin G by permeation could be reduced from 4.3% to 3.4% by a repeated addition of penicillin G. 相似文献
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核因子κB(NF-κB)是细胞内重要的转录因子,其介导的细胞信号转导通路在细胞凋亡中的作用是国内外研究的热点.为了筛选NF-κB通路相关新基因,建立了基于细胞水平的报告基因高通量筛选模型.利用双荧光素酶报告系统检测报告基因荧光素酶活性,通过对构建的439个人类未知功能基因的筛选,获得了一批激活NF-κB信号通路的功能基因,其中基因TMEM9B可以明显激活NF-κB通路.进一步实验显示TMEM9B激活NF-κB通路呈明显剂量依赖性,Western blot及EMSA实验证实,TMEM9B能够促进胞质内NF-κB的抑制分子IκBα的降解,并促使NF-κB由胞质向胞核转移,同时流式细胞术实验发现TMEM9B可引起293T和HeLa细胞的凋亡.总之,所建立的基于细胞水平的NF-κB通路筛选模型稳定高效,筛选并验证TMEM9B可明显激活NF-κB信号转导通路,并从而引起细胞凋亡. 相似文献