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Dual bidirectional promoters at the mouse dhfr locus: cloning and characterization of two mRNA classes of the divergently transcribed Rep-1 gene. 总被引:30,自引:11,他引:19
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J P Linton J Y Yen E Selby Z Chen J M Chinsky K Liu R E Kellems G F Crouse 《Molecular and cellular biology》1989,9(7):3058-3072
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Structural organization of the human CaMIII calmodulin gene 总被引:4,自引:0,他引:4
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A gene encoding rat cholecystokinin. Isolation, nucleotide sequence, and promoter activity 总被引:14,自引:0,他引:14
R J Deschenes R S Haun C L Funckes J E Dixon 《The Journal of biological chemistry》1985,260(2):1280-1286
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A Visvikis A Pawlak M J Accaoui K Ichino H Leh G Guellaen M Wellman 《European journal of biochemistry》2001,268(2):317-325
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J Pollock V J Baule C B Rich C D Ginsburg S W Curtiss J A Foster 《The Journal of biological chemistry》1990,265(7):3697-3702
Studies from several laboratories have demonstrated the existence of multiple tropoelasting mRNAs and protein isoforms. The present study was designed to examine the developmental expression of a specific tropoelastin mRNA, its encoded isoform, and the fate of that isoform in the extracellular matrix. A chick genomic DNA library was screened with a chick tropoelastin cDNA. Seven unique, overlapping clones spanning 39 kilobases were isolated. A synthetic oligonucleotide complementary to a variable tropoelastin mRNA sequence was used to identify a 1.5-kilobase PstI-BamHI genomic fragment. Nucleotide sequence data revealed that the putative exon was surrounded by intron sequences possessing canonical splice sites at the exon/intron borders. Using both immunologic and molecular probes specific to the tropoelastin isoform and mRNA, quantitative protein and RNA analyses were performed. Results demonstrate that total tropoelastin mRNAs increased significantly during aortic embryogenesis whereas the amount of mRNA containing the variable exon remained relatively constant. The amount of total tropoelastins within the same developmental period reflect the level of total tropoelastin mRNA. The amount of the tropoelastin isoform containing the variable exon essentially mirrored the corresponding mRNA with the exception that a decrease in the isoform at day 15 was not seen in the mRNA level. Immunoelectron micrographs of 13-day chick aortic tissue using both total and isoform-specific antisera showed ultrastructural localization to definable elastic fibers. Antibodies to the variable tropoelastin isoform occurred preferentially at sites where elastic fiber microfibril structures were evident. 相似文献