共查询到20条相似文献,搜索用时 15 毫秒
1.
Velasquez-Orta SB Yu E Katuri KP Head IM Curtis TP Scott K 《Applied microbiology and biotechnology》2011,90(2):789-798
This study determined the influence of substrate degradation on power generation in microbial fuel cells (MFCs) and microbial
community selection on the anode. Air cathode MFCs were fed synthetic medium containing different substrates (acetate, glucose
and starch) using primary clarifier sewage as source of electroactive bacteria. The complexity of the substrate affected the
MFC performance both for power generation and COD removal. Power output decreased with an increase in substrate complexity
from 99 ± 2 mW m−2 for acetate to 4 ± 2 mW m−2 for starch. The organic matter removal and coulombic efficiency (CE) of MFCs with acetate and glucose (82% of COD removal
and 26% CE) were greater than MFCs using starch (60% of COD removal and 19% of CE). The combined hydrolysis–fermentation rate
obtained (0.0024 h−1) was considerably lower than the fermentation rate (0.018 h−1), indicating that hydrolysis of complex compounds limits current output over fermentation. Statistical analysis of microbial
community fingerprints, developed on the anode, showed that microbial communities were enriched according to the type of substrate
used. Microbial communities producing high power outputs (fed acetate) clustered separately from bacterial communities producing
low power outputs (fed complex compounds). 相似文献
2.
Liping Huang Jingwen Chen Xie Quan Fenglin Yang 《Bioprocess and biosystems engineering》2010,33(8):937-945
Enhancement of Cr (VI) reduction rate and power production from biocathode microbial fuel cells (MFCs) was achieved using
indigenous bacteria from Cr (VI)-contaminated site as inoculum and MFC architecture with a relatively large cathode-specific
surface area of 340–900 m2 m−3. A specific Cr (VI) reduction rate of 2.4 ± 0.2 mg g−1VSS h−1 and a power production of 2.4 ± 0.1 W m−3 at a current density of 6.9 A m−3 were simultaneously achieved at an initial Cr (VI) concentration of 39.2 mg L−1. Initial Cr (VI) concentration and solution conductivity affected Cr (VI) reduction rate, power production and coulombic
efficiency. These findings demonstrate the importance of inoculation and MFC architecture in the enhancement of Cr (VI) reduction
rate and power production. This study is a beneficial attempt to improve the efficiency of biocathode MFCs and provide a good
candidate of bioremediation process for Cr (VI)-contaminated sites. 相似文献
3.
Sharon B. Velasquez-Orta Ian M. Head Thomas P. Curtis Keith Scott Jonathan R. Lloyd Harald von Canstein 《Applied microbiology and biotechnology》2010,85(5):1373-1381
The effect of electron shuttles on electron transfer to microbial fuel cell (MFC) anodes was studied in systems where direct
contact with the anode was precluded. MFCs were inoculated with Shewanella cells, and flavins used as the electron shuttling compound. In MFCs with no added electron shuttles, flavin concentrations
monitored in the MFCs' bulk liquid increased continuously with FMN as the predominant flavin. The maximum concentrations were
0.6 μM for flavin mononucleotide and 0.2 μM for riboflavin. In MFCs with added flavins, micro-molar concentrations were shown
to increase current and power output. The peak current was at least four times higher in MFCs with high concentrations of
flavins (4.5–5.5 μM) than in MFCs with low concentrations (0.2–0.6 μM). Although high power outputs (around 150 mW/m2) were achieved in MFCs with high concentrations of flavins, a Clostridium-like bacterium along with other reactor limitations affected overall coulombic efficiencies (CE) obtained, achieving a maximum
CE of 13%. Electron shuttle compounds (flavins) permitted bacteria to utilise a remote electron acceptor (anode) that was
not accessible to the cells allowing current production until the electron donor (lactate) was consumed. 相似文献
4.
A fast and convenient bacterial immobilization method was proposed as an attempt to improve the anode efficiency of a microbial
fuel cell, in which bacteria were entrapped into carbon nanoparticle matrix. The direct electron transfer from the entrapped
bacterial cells to the anode was verified using cyclic voltammogram (CV). Using the immobilized bioanode, the start-up time
of the MFC was greatly reduced. Meanwhile, the maximum power density of 1,947 mW m−2 with the modified anode was much higher than that with the biofilm-based carbon cloth anode (1,479 mW m−2). Impedance measurements suggested that performance improvement resulted from the decrease in charge transfer and diffusion
resistances. The results demonstrated that bacteria immobilization using carbon nanoparticle matrix was a simple and efficient
approach for improving the anodes performances in MFCs. 相似文献
5.
Lefebvre O Uzabiaga A Chang IS Kim BH Ng HY 《Applied microbiology and biotechnology》2011,89(2):259-270
As the microbial fuel cell (MFC) technology is getting nearer to practical applications such as wastewater treatment, it is
crucial to consider the different aspects that will make this technology viable in the future. In this paper, we provide information
about the specifications of an energy self-sufficient MFC system as a basis to extrapolate on the potential benefits and limits
of a future MFC-based wastewater treatment plant. We particularly emphasize on the importance of two crucial parameters that
characterize an MFC: its electromotive force (E
emf) and its internal resistance (R
int). A numerical projection using state-of-art values (E
emf = 0.8 V and R
int = 5 Ω) emphasized on the difficulty at this moment to reach self-sufficiency using a reasonable number of MFCs at the laboratory
scale. We found that a realistic number of MFCs to provide enough voltage (=5 V) at a sufficient current (=0.8 A) to power
a pump requiring 4 W would be of 13 MFCs in series and 10 stacks of MFCs in parallel, resulting in a total number of 130 MFCs.
That would result in a treatment capacity of 144 L of domestic wastewater (0.5 g-COD L−1) per day. The total MFC system would be characterized by an internal resistance of 6.5 Ω. 相似文献
6.
Xue Xia Xiao-xin Cao Peng Liang Xia Huang Su-ping Yang Gen-gui Zhao 《Applied microbiology and biotechnology》2010,87(1):383-390
As electrochemically active bacteria play an important role in microbial fuel cells (MFCs), it is necessary to get a comprehensive
understanding of their electrogenesis mechanisms. In this study, a new electrochemically active bacterium, Klebsiella sp. ME17, was employed into an “H” typed MFC for electrogenesis, with glucose as the electron donor. The maximum power density
was 1,209 mW/m2 at a resistance of 340 Ω and the maximum current was 1.47 mA. Given the original anode medium, fresh medium, and the supernatant
of the anode medium in the same MFC, respectively, the polarization curves illustrated that the strain produced mediators
to promote extracellular electron transfer. The anode medium supernatant was electrochemically active, based on cyclic voltammogram,
and the supernatant was very likely to contain quinone-like substances, as indicated by spectrophotometric and excitation–emission
matrix fluorescence spectroscopy analysis. Further investigation on the color and ultraviolet absorbance at 254 nm of the
filtered anode medium showed that the redox states of mediators strongly associated with the electricity generation states
in MFCs. 相似文献
7.
Guillermo Quijano María Hernandez Frédéric Thalasso Raúl Muñoz Santiago Villaverde 《Applied microbiology and biotechnology》2009,82(5):829-836
Operation of microbial electrolysis cells (MECs) without an ion exchange membrane could help to lower the construction costs
while lowering the ohmic cell resistance and improving MEC conversion rates by minimizing the pH gradient between anode and
cathode. In this research, we demonstrate that membraneless MECs with plain graphite can be operated for methane production
without pH adjustment and that the ohmic cell resistance could be lowered with approximately 50% by removing the cation exchange
membrane. As a result, the current production increased from 66 ± 2 to 156 ± 1 A m−3 MEC by removing the membrane with an applied voltage of −0.8 V. Methane was the main energetic product despite continuous
operation under carbonate-limited and slightly acidified conditions (pH 6.1–6.2). Our results suggest that continuous production
of hydrogen in membraneless MECs will be challenging since methane production might not be avoided easily. The electrical
energy invested was not always completely recovered under the form of an energy-rich biogas; however, our results indicate
that membraneless MECs might be a viable polishing step for the treatment of the effluent of anaerobic digesters as methane
was produced under low organic loading conditions and at room temperature.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
8.
Scaling up microbial fuel cells (MFCs) requires the development of compact reactors with multiple electrodes. A scalable single
chamber MFC (130 mL), with multiple graphite fiber brush anodes and a single air-cathode cathode chamber (27 m2/m3), was designed with a separator electrode assembly (SEA) to minimize electrode spacing. The maximum voltage produced in fed-batch
operation was 0.65 V (1,000 Ω) with a textile separator, compared to only 0.18 V with a glass fiber separator due to short-circuiting
by anode bristles through this separator with the cathode. The maximum power density was 975 mW/m2, with an overall chemical oxygen demand (COD) removal of >90% and a maximum coulombic efficiency (CE) of 53% (50 Ω resistor).
When the reactor was switched to continuous flow operation at a hydraulic retention time (HRT) of 8 h, the cell voltage was
0.21 ± 0.04 V, with a very high CE = 85%. Voltage was reduced to 0.13 ± 0.03 V at a longer HRT = 16 h due to a lower average
COD concentration, and the CE (80%) decreased slightly with increased oxygen intrusion into the reactor per amount of COD
removed. Total internal resistance was 33 Ω, with a solution resistance of 2 Ω. These results show that the SEA type MFC can
produce stable power and a high CE, making it useful for future continuous flow treatment using actual wastewaters. 相似文献
9.
Nitrite accumulates during biological denitrification processes when carbon sources are insufficient. Acetate, methanol, and
ethanol were investigated as supplementary carbon sources in the nitrite denitrification process using biogranules. Without
supplementary external electron donors (control), the biogranules degraded 200 mg l−1 nitrite at a rate of 0.27 mg NO2–N g−1 VSS h−1. Notably, 1,500 mg l−1 acetate and 700 mg l−1 methanol or ethanol enhanced denitrification rates for 200 mg l−1 nitrite at 2.07, 1.20, and 1.60 mg NO2–N g−1 VSS h−1, respectively; these rates were significantly higher than that of the control. The sodium dodecyl sulfate polyacrylamide
gel electrophoresis of the nitrite reductase (NiR) enzyme identified three prominent bands with molecular weights of 37–41 kDa.
A linear correlation existed between incremental denitrification rates and incremental activity of the NiR enzyme. The NiR
enzyme activity was enhanced by the supplementary carbon sources, thereby increasing the nitrite denitrification rate. The
capacity of supplementary carbon source on enhancing NiR enzyme activity follows: methanol > acetate > ethanol on molar basis
or acetate > ethanol > methanol on an added weight basis. 相似文献
10.
Two types of rapidly biodegradable vegetable products (the liquid fraction of clover and the glycerol-containing sidestream
from biodiesel production) were selected for anodic oxidation in microbial fuel cells (MFC) equipped with a biocathode. As
benchmark references, five abundant amino-acids in plant sap (l-glutamine, l-glutamic acid, l-asparagine, l-aspartic acid and l-alanine) were tested separately. Their performance was in the same order of magnitude of clover sap oxidation (145–225 A
m−3 MFC; 39–95 W m−3 MFC). Glycerol oxidation resulted in competitive current and power outputs (111 A m−3 MFC; 23 W m−3 MFC). 相似文献
11.
A mediator-less three-stage two-chamber microbial fuel cell (MFC) system was developed and operated continuously for more
than 1.5 years to evaluate continuous power generation while treating artificial wastewater containing glucose (10 mM) concurrently.
A stable power density of 28 W/m3 was attained with an anode hydraulic retention time of 4.5 h and phosphate buffer as the cathode electrolyte. An overall
dissolved organic carbon removal ratio was about 85%, and coulombic efficiency was about 46% in this MFC system. We also analyzed
the microbial community structure of anode biofilms in each MFC. Since the environment in each MFC was different due to passing
on the products to the next MFC in series, the microbial community structure was different accordingly. The anode biofilm
in the first MFC consisted mainly of bacteria belonging to the Gammaproteobacteria, identified as Aeromonas sp., while the Firmicutes dominated the anode biofilms in the second and third MFCs that were mainly fed with acetate. Cyclic
voltammetric results supported the presence of a redox compound(s) associated with the anode biofilm matrix, rather than mobile
(dissolved) forms, which could be responsible for the electron transfer to the anode. Scanning electron microscopy revealed
that the anode biofilms were comprised of morphologically different cells that were firmly attached on the anode surface and
interconnected each other with anchor-like filamentous appendages, which might support the results of cyclic voltammetry.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
12.
Liang Liu Fang-bai Li Chun-hua Feng Xiang-zhong Li 《Applied microbiology and biotechnology》2009,85(1):175-183
Microbial fuel cells (MFCs) were constructed using azo dyes as the cathode oxidants to accept the electrons produced from
the respiration of Klebsiella pneumoniae strain L17 in the anode. Experimental results showed that a methyl orange (MO)-feeding MFC produced a comparable performance
against that of an air-based one at pH 3.0 and that azo dyes including MO, Orange I, and Orange II could be successfully degraded
in such cathodes. The reaction rate constant (k) of azo dye reduction was positively correlated with the power output which was highly dependent on the catholyte pH and
the dye molecular structure. When pH was varied from 3.0 to 9.0, the k value in relation to MO degradation decreased from 0.298 to 0.016 μmol min−1, and the maximum power density decreased from 34.77 to 1.51 mW m−2. The performances of the MFC fed with different azo dyes can be ranked from good to poor as MO > Orange I > Orange II. Furthermore,
the cyclic voltammograms of azo dyes disclosed that the pH and the dye structure determined their redox potentials. A higher
redox potential corresponded to a higher reaction rate. 相似文献
13.
Simultaneous electricity generation and selenium removal was evaluated in single-chamber microbial fuel cells (MFCs) with
acetate and glucose as carbon sources. Power output was not affected by selenite up to 125 mg l−1 with glucose as substrate. Coulombic efficiencies of MFCs with glucose increased from 25% to 38% at 150 mg Se l−1. About 99% of 50 and 200 mg Se l−1 selenite was removed in 48 and 72 h for MFCs fed with acetate and glucose, respectively, demonstrating the potential of using
MFC technology for Se remediation. 相似文献
14.
Simultaneous nutrients and carbon removal during pretreated swine slurry degradation in a tubular biofilm photobioreactor 总被引:1,自引:0,他引:1
Ignacio de Godos Cristina González Eloy Becares Pedro A. García-Encina Raúl Muñoz 《Applied microbiology and biotechnology》2009,82(1):187-194
The biodegradation potential of an innovative enclosed tubular biofilm photobioreactor inoculated with a Chlorella sorokiniana strain and an acclimated activated sludge consortium was evaluated under continuous illumination and increasing pretreated
(centrifuged) swine slurry loading rates. This photobioreactor configuration provided simultaneous and efficient carbon, nitrogen,
and phosphorous treatment in a single-stage process at sustained nitrogen and phosphorous removals efficiencies ranging from
94% to 100% and 70–90%, respectively. Maximum total organic carbon (TOC), NH4
+, and PO4
3− removal rates of 80 ± 5 g C mr
−3 day−1, 89 ± 5 g N mr
−3 day−1, and 13 ± 3 g P mr
−3 day−1, respectively, were recorded at the highest swine slurry loadings (TOC of 1,247 ± 62 mg L−1, N–NH4
+ of 656 ± 37 mg L−1, P–PO4
3+ of 117 ± 19 mg L−1, and 7 days of hydraulic retention time). The unusual substrates diffusional pathways established within the phototrophic
biofilm (photosynthetic O2 and TOC/NH4
+ diffusing from opposite sides of the biofilm) allowed both the occurrence of a simultaneous denitrification/nitrification
process at the highest swine slurry loading rate and the protection of microalgae from any potential inhibitory effect mediated
by the combination of high pH and high NH3 concentrations. In addition, this biofilm-based photobioreactor supported efficient biomass retention (>92% of the biomass
generated during the pretreated swine slurry biodegradation). 相似文献
15.
Malaysia is the world’s leading producer of palm oil products that contribute US$ 7.5 billion in export revenues. Like any
other agro-based industries, it generates waste that could be utilized as a source of organic nutrients for microalgae culture.
Present investigation delves upon Isochrysis sp. culture in POME modified medium and its utilization as a supplement to Nanochloropsis sp. in rotifer cultures. The culture conditions were optimized using a 1 L photobioreactor (Temp: 23°C, illumination: 180 ∼ 200 μmol
photons m−2s−1, n = 6) and scaled up to 10 L outdoor system (Temp: 26–29°C, illumination: 50 ∼ 180 μmol photons m−2s−1, n = 3). Algal growth rate in photobioreactor (μ = 0.0363 h−1) was 55% higher compared to outdoor culture (μ = 0.0163 h−1), but biomass production was 1.3 times higher in outdoor culture (Outdoor = 91.7 mg m−2d−1; Photobioreactor = 69 mg m−2d−1). Outdoor culture produced 18% higher lipid; while total fatty acids (FA) was not significantly affected by the change in
culture systems as both cultures yield almost similar concentrations of fatty acids per gram of sample (photobioreactor = 119.17 mg
g−1; outdoor culture = 104.50 mg g−1); however, outdoor cultured Isochrysis sp. had 26% more polyunsaturated fatty acids (PUFAs). Rotifers cultured in Isochrysis sp./ Nanochloropsis sp. (1:1, v/v) mixture gave similar growth rate as 100% Nanochoropsis sp. culture (μ = 0.40 d−1), but had 45% higher counts of rotifers with eggs (t = 7, maximum). The Isochrysis sp. culture successfully lowered the nitrate (46%) and orthophosphate (83%) during outdoor culture. 相似文献
16.
Raúl Muñoz María Hernández Ana Segura Joao Gouveia Antonia Rojas Juan Luis Ramos Santiago Villaverde 《Applied microbiology and biotechnology》2009,83(1):189-198
The long-term performance and stability of Pseudomonas putida mt-2 cultures, a toluene-sensitive strain harboring the genes responsible for toluene biodegradation in the archetypal plasmid
pWW0, was investigated in a chemostat bioreactor functioning under real case operating conditions. The process was operated
at a dilution rate of 0.1 h−1 under toluene loading rates of 259 ± 23 and 801 ± 78 g m−3 h−1 (inlet toluene concentrations of 3.5 and 10.9 g m−3, respectively). Despite the deleterious effects of toluene and its degradation intermediates, the phenotype of this sensitive
P. putida culture rapidly recovered from a 95% Tol− population at day 4 to approx. 100% Tol+ cells from day 13 onward, sustaining elimination capacities of 232 ± 10 g m−3 h−1 at 3.5 g Tol m−3 and 377 ± 13 g m−3 h−1 at 10.9 g Tol m−3, which were comparable to those achieved by highly tolerant strains such as P. putida DOT T1E and P. putida F1 under identical experimental conditions. Only one type of Tol− variant, harboring a TOL-like plasmid with a 38.5 kb deletion (containing the upper and meta operons for toluene biodegradation), was identified. 相似文献
17.
The purpose of this study was to determine the effect of enrichment procedure on the performance and microbial diversity of
an air-cathode microbial fuel cell (MFC) which was explored for simultaneous azo dye decolorization and electricity generation.
Two different enrichment procedures in which glucose and Congo red were added into the MFCs sequentially (EP1) or simultaneously
(EP2) were tested by operating parallel MFCs independently for more than 6 months. The power density, electrode potential,
Congo red decolorization, biofilm morphology, and bacterial diversity of the MFCs under the two enrichment procedures were
compared and investigated. The results showed that the enrichment procedures have a negligible effect on the dye decolorization,
but significantly affected the electricity generation. More than 90% decolorization at dye concentration of 300 mg/L was achieved
within 170 h for the two tested enrichment procedures. However, the MFC with EP2 achieved a maximum power density of 192 mW/m2, which was 75% higher than that of the MFC with EP1 (110 mW/m2). The depressed surfaces of the bacteria in the MFC with EP1 indicated the allergic response caused by the subsequent addition
of Congo red. 16S rRNA sequencing analysis demonstrated a phylogenetic diversity in the communities of the anode biofilm and
showed clear differences between the anode-attached populations in the MFCs with a different enrichment procedure. This study
suggests that the enrichment procedure is important for the MFC explored for simultaneous dye decolorization and electricity
generation. 相似文献
18.
The zooxanthellate octocoral Sinularia flexibilis is a producer of potential pharmaceutically important metabolites such as antimicrobial and cytotoxic substances. Controlled
rearing of the coral, as an alternative for commercial exploitation of these compounds, requires the study of species-specific
growth requirements. In this study, phototrophic vs. heterotrophic daily energy demands of S. flexibilis was investigated through light and Artemia feeding trials in the laboratory. Rate of photosynthetic oxygen by zooxanthellae in light (≈200 μmol quanta m−2 s−1) was measured for the coral colonies with and without feeding on Artemia nauplii. Respiratory oxygen was measured in the dark, again with and without Artemia nauplii. Photosynthesis–irradiance curve at light intensities of 0, 50, 100, 200, and 400 μmol quanta m−2 s−1 showed an increase in photosynthetic oxygen production up to a light intensity between 100 and 200 μmol quanta m−2 s−1. The photosynthesis to respiration ratio (P/R > 1) confirmed phototrophy of S. flexibilis. Both fed and non-fed colonies in the light showed high carbon contribution by zooxanthellae to animal (host) respiration
values of 111–127%. Carbon energy equivalents allocated to the coral growth averaged 6–12% of total photosynthesis energy
(mg C g
−
1 buoyant weight day
−
1) and about 0.02% of the total daily radiant energy. “Light utilization efficiency (ε)” estimated an average ε value of 75%
12 h
−
1 for coral practical energetics. This study shows that besides a fundamental role of phototrophy vs. heterotrophy in daily
energy budget of S. flexibilis, an efficient fraction of irradiance is converted to useable energy. 相似文献
19.
Jonny E. Scherwinski-Pereira Rodrigo S. da Guedes Paulo César P. FerminoJr Tatiane L. Silva Frederico Henrique S. Costa 《In vitro cellular & developmental biology. Plant》2010,46(4):378-385
An efficient procedure has been developed for inducing somatic embryogenesis and regeneration of plants from tissue cultures
of oil palm (Elaeis guineensis Jacq.). Thin transverse sections (thin cell layer explants) of different position in the shoot apex and leaf sheath of oil
palm were cultivated in Murashige and Skoog (MS) (Physiol Plant 15:473–497, 1962) medium supplemented with 0–450 μM picloram and 2,4-D with 3.0% sucrose, 500 mg L−1 glutamine, and 0.3 g L−1 activated charcoal and gelled with 2.5 g L−1 Phytagel. Embryogenic calluses were evaluated 12 wk after inoculation. Picloram (450 μM) was effective in inducing embryogenic
calluses in 41.5% of the basal explants. Embryogenic calluses were maintained on a maturation medium composed of basal media,
plus 0.6 μM NAA and 12.30 μM 2iP, 0.3 g L−1 activated charcoal, and 500 mg L−1 glutamine, with subcultures at 4-wk intervals. Somatic embryos were converted to plants on MS medium with macro- and micronutrients
at half-strength, 2% sucrose, and 1.0 g L−1 activated charcoal and gelled with 2.5 g L−1 Phytagel. 相似文献
20.
V. I. Kolmakov O. V. Anishchenko E. A. Ivanova M. I. Gladyshev N. N. Sushchik 《Journal of applied phycology》2008,20(3):289-297
Periphyton (epilithon) gross primary production (GPP) was estimated using the DCMU-fluorescence method in the Yenisei River.
In the unshaded littoral zone, chlorophyll a concentration (Chl a) and GPP value varied from 0.83 to 973.74 mg m−2and 2–304,425 O2 m−2 day−1 (0.64–95 133 mg C m−2 day−1), respectively. Positive significant correlation (r = 0.8) between daily GPP and periphyton Chl a was found. Average ratio GPP:Chl a for periphyton was 36.36 mg C mg Chl a m−2 day−1. The obtained GPP values for the Yenisei River have a high significant correlation with values predicted by a conventional
empirical model for stream periphyton. We concluded that the DCMU-fluorescence method can be successfully used for measuring
of gross primary production of stream phytoperiphyton at least as another useful tool for such studies. 相似文献