Biovolume as a tool in biomass determination of Oligochaeta and Chironomidae

• 1 We used biovolume as a simple non-destructive means for estimating biomass for several tubificid and chironomid species. Biovolumes were determined by treating the organisms as cylinders and measuring body lengths and diameters; estimates were verified by measuring the displacement of water by live organisms in a 5-ml burette. In addition, factors were determined for the conversion of biovolume (V) into wet weight (WW), dry weight (DW) and ash-free dry weight (AFDW).
• 2 For six tubificid species, regression functions between the diameter of segment XI (D₁₁) and the biovolume enabled the prediction of any biovolume merely by measuring D₁₁. This method, however, underestimated biovolumes of Branchiura sowerbyi by 38–47% compared with burette measurements. This difference is largely attributable to the mountant we used during identification.
• 3 For ten chironomid species a linear relation was found between body length and the diameter of abdominal segment 5. The biovolume of Chironomus muratensis was underestimated by an average of 12% compared with burette measurements.
• 4 Specific gravity (WW:V) amounted to 1.07 and 1.05, DW:WW ratios were 0.20 and 0.142 and AFDW:WW ratios were 0.182 and 0.129 for Oligochaeta and Chironomidae, respectively.
• 5 The biovolume method is not yet very accurate for Oligochaeta, but to our knowledge, it is the only practical method that can be used in natural polyspecific communities that need to be treated with elucidating media for identification. For those Chironomidae that need no elucidation for identification, this method is time saving and just as accurate as the direct body shape—biomass method.

     

The impact of small chironomid grazers on epiphytic algal abundance and dispersion

• 1 A combination of field and laboratory experiments was used to assess the impact of chironomid grazers on taxonomic composition, abundance and dispersion of epiphytic algal assemblages.
• 2 In the laboratory, Psectrodadius sp. reduced the biovolume of algal species preferred as food and increased the degree of clumping of non-preferred species. Thienemanniella cf. fusca had both positive and negative effects (depending on the algal species) on the biovolumes of algal species preferred as food and increased the degree of clumping of non-preferred species.
• 3 In field exclosures, no effect of removal of chironomid larvae from the grazer assemblage could be detected in autumn or winter experiments. A third, longer removal experiment, conducted in summer, resulted in increased biovolumes of edible Cosmarium spp. and Aphanocapsa spp., preferred foods of chironomid larvae. Biovolumes of Lyngbya sp., Eulbochaete spp. and Oedogortium spp., filamentous taxa used extensively in larval case construction, also increased. Chironomid larvae had no effect on total algal biovolume or biovolume of large unicellular algae.
• 4 Chironomid larvae can influence epiphytic algal assemblages through selective grazing by reducing the biovolumes of preferred foods and through case-building activity by reducing the biovolumes of construction materials.

     

Phenology and bathymetric distribution of the profundal chironomid fauna in Starnberger See (F.R. Germany)

Results of the first long-term investigation of the profundal chironomid fauna of Starnberger See, a prealpine lake are reported. Data are presented on the qualitative and quantitative composition of the chironomid fauna in the profundal of Starnberger See, on the spatial variation, and on the phenology of the dominant species, based on pupal occurrence and the seasonal distribution of larval instars. Twenty-five distinct chironomid taxa could be recorded in the profundal zone. Typical inhabitants of the profundal zone of Starnberger See are the larvae of the genus Micropsectra. The maximum abundance of Micropsectra larvae were found at 60 m with 5644 Ind.m⁻². Abundance, distribution and phenology of the dominant chironomid species are discussed in comparison with other lakes.     

Estimation of larval dry weight of Chaoborus americanus

A method for estimating larval dry weight as a function of tracheal front air sac length and instar was developed for second, third, and fourth instar Chaoborus americanus using linear regression analysis. The following equation: where W = larval dry weight (μg), L = front air sac length (mm), a = the y-intercept, and b = the slope provided accurate estimates of larval dry weight for three C. americanus populations. No detectable change in size occurred in the air sacs of larvae preserved for one year in 5% formalin. This indicates that air sacs can be used to accurately estimate larval dry weight for preserved as well as living specimens.     

Seasonal size variation in the predatory cladoceran Bythotrephes cederstroemii in Lake Michigan

• 1 Dry weight, body length and spine length were measured for the exotic cladoceran Bythotrephes cederstroemii collected from offshore and inshore stations in southeastern Lake Michigan. Average dry weight of each developmental stage exhibited seasonal variation by a factor of more than 5.
• 2 Mean dry weight of Bythotrephes was closely correlated with water temperature. Contrary to the inverse relationship between water temperature and body size frequently observed for other invertebrates, the dry weight of Bythotrephes increased at higher ambient temperatures.
• 3 No significant correlation was observed between abundances of major zooplankton taxa and the dry weight of Bythotrephes. An indirect effect of temperature on prey consumption may cause seasonal variation in dry weight of Bythotrephes in Lake Michigan.
• 4 Distances between adjacent pairs of barbs, added to the caudal spine with each moult, are significantly shorter in Bythotrephes which produce resting eggs. Less material investment in the exoskeleton of sexually reproducing females was observed in favour of growth and reproduction.

     

Ecology of body size in Drosophila buzzatii: untangling the effects of temperature and nutrition

Abstract.

• 1 A method of separating the effects of two important determinants of body size in natural populations, temperature of larval development and level of larval nutrition, by making measurements of thorax length and wing length of adult flies is investigated.
• 2 I show that at any given time variation in body size of Drosophila buzzatii from two sites in eastern Australia is determined primarily by variation in the quality of nutrition available to larvae.
• 3 Throughout the year adult flies are consistently at least 25% smaller in volume than predicted for optimal nutrition at their predicted temperature of larval development.
• 4 Nutritional stress is therefore a year-round problem for these flies.
• 5 Measurements of adult flies emerging from individual breeding substrates (rotting cactus cladodes) show that there is substantial variation among these substrates in the nutrition available to larvae.
• 6 This method will allow study of spatial and temporal variation in the temperature of larval substrates and in the nutritional resources available to flies in natural populations.

     

Larval age differences and competition between Drosophila aldrichi and D.buzzatii

Abstract.

• 1 Competitive interactions among larvae of Drosophila species using the same habitat will usually involve individuals varying in age and size. Older, larger larvae might be expected to outcompete younger, smaller larvae.
• 2 Newly hatched laryae of Drosophila aldrichi and D.buzzatii were placed on induced cactus necroses (simulating the natural habitat), either simultaneously, or with a 1 or 2 day difference in the time at which individuals of the other species were added.
• 3 Evidence for competitive differences with larval age were found. In both species, body weight was significantly higher for those larvae added first than for later addition larvae, and a longer time to complete development was required for those larvae added 2 days later. However, pre-adult viability of D.aldrichi was significantly higher for larvae started 1 or 2 days after larvae of D.buzzatii. For D.buzzatii, viability was lowest for larvae started simultaneously with D.aldrichi, but not different between those started either before or after D.aldrichi.
• 4 Considering all three traits, no clear advantage for the early addition larvae was observed.

     

Development of two forensically important blowfly species (Chrysomya megacephala and Chrysomya rufifacies) (Diptera: Calliphoridae) at four temperatures in India

The development of the Oriental latrine fly, Chrysomya megacephala (Fabricius), and hairy maggot blowfly, C. rufifacies (Macquart) (Diptera: Calliphoridae), was studied at four different temperatures (22°C, 25°C, 29°C and 31°C) in order to draw correlations between larval age, body length and body dry weight. The mean larval body length increased steadily from a minimum of 1.4 mm for C. megacephala and 1.8 mm for C. rufifacies to a maximum of 17.4 mm for C. megacephala and 15.9 mm for C. rufifacies at different temperatures. Similarly, the mean dry weight increased steadily from a minimum of 0.0007 g for C. megacephala (second instar) and 0.0008 g for C. rufifacies (second instar) to a maximum of 0.0290 g for C. megacephala and 0.0270 g for C. rufifacies at different temperatures. Entomological evidence is often used to estimate the minimum postmortem interval (mPMI) and both of these species are important from a forensic point of view. Graphs of age of larvae vs. body length and age of larvae vs. dry body weight at different temperatures can be used to estimate the larval age of these two species.     

Purification and characterization of glycogen phosphorylase A and B from the freeze-avoiding gall moth larvae Epiblema scudderiana

The active a and inactive b forms of glycogen phosphorylase from cold-hardy larvae of the gall moth, Epiblema scudderiana, were purified using DEAE⁺ ion exchange and 3-5-AMP-agarose affinity chromatography. Maximum activities for glycogen phosphorylases a and b were 6.3±0.74 and 2.7±0.87 mol glucose-1-P·min^-1·g wet weight^-1, respectively, in -4°C-acclimated larvae. Final specific activities of the purified enzymes were 396 and 82 units·mg protein^-1, respectively. Both enzymes were dimers with native molecular weights of 215000±18000 for glycogen phosphorylase a and 209000±15000 for glycogen phosphorylase b; the subunit molecular weight of both forms was 87000±2000. Both enzymes showed pH optima of 7.5 at 22°C and a break in the Arrhenius relationship with a two- to four-fold increase in activation energy below 10°C. Michaelis constant values for glycogen at 22°C were 0.12±0.004 mg·ml^-1 for glycogen phosphorylase a and 0.87±0.034 mg·ml^-1 for glycogen phosphorylase b; the Michaelis constant for inorganic phosphate was 6.5±0.07 mmol·l^-1 for glycogen phosphorylase a and 23.6 mmol·l^-1 for glycogen phosphorylase b. Glycogen phosphorylase b was activated by adenosine monophosphate with a K _a of 0.176±0.004 mmol·l^-1. Michaelis constant and K _a values decreased by two- to fivefold at 5°C compared with 22°C. Glycerol had a positive effect on the Michaelis constant for glycogen for glycogen phosphorylase a at intermediate concentrations (0.5 mol·l^-1) but was inhibitory to both enzyme forms at high concentrations (2 mol·l^-1). Glycerol production as a cryoprotectant in E. scudderiana larvae is facilitated by the low temperature-simulated glycogen phosphorylase b to glycogen phosphorylase a conversion and by positive effects of low temperature on the kinetic properties of glycogen phosphorylase a. Enzyme shut-down when polyol synthesis is complete appears to be aided by strong inhibitory effects of glycerol and KCl on glycogen phosphorylase b.Abbreviations E _a activation energy - GPa glycogen phosphorylase a - GPb glycogen phosphorylase b - h Hill coefficient - I ₅₀ concentration of inhibitor that reduces enzymes velocity by 50% - K _a concentration of activator that produces half-maximal activation of enzyme activity - K _m Michaelis-Menten substrate affinity constant - MW molecular weight - PEG polyethylene glycol - P_i morganic phosphate - SDS PAGE sodium dodecyl sulphate polyacrylamide gel electrophoresis - V _max enzyme maximal velocity     

The food and daily food consumption of lacustrine 0+ perch, Perca fluviatilis L.

SUMMARY.

• 1 The diet and daily food consumption (C_t) of 0+ perch were investigated in two shallow Scottish lakes.
• 2 The first food comprised the rotifer Keratella sp., Volvox sp., copepod nauplii and stage I copepodites of Cyclops strenuus abyssorum Sars. Larger copepodites and adults of C. s. abyssorum and Diaptomus gracilis Sars were consumed as larvae grew and cladocerans were also eaten by larvae >11 mm. The diet of juveniles included a wider range of cladocerans and benthic invertebrates. Cannibalism was not observed among 0+ perch.
• 3 The range of food items taken by larvae increased with length, and the size of food particles ingested was governed by jaw gape which was linearly related to fish length.
• 4 Larvae fed during daylight hours with feeding maxima in midmorning and the evening.
• 5 The arithmetic (constant) rate of evacuation R of the digestive tract of larvae was 0.59—1.16% of body weight h^?1 and exponential R was 0.21–0.33% h^?1. Daily C_t calculated using an arithmetic model was 14.2–23.4% body weight at water temperatures of 12.5–18.2°C, and 23.9–40.3% utilizing an exponential method. For larvae of 9 mm. this was 46 μg dry weight day^?1 and 148 by an exponential model. Food conversion efficiency was 37–72% (arithmetic model) and 21–45% (exponential model).

     

Allometry between leg and body length of insects: lack of support for the size–grain hypothesis

• 1 The size–grain hypothesis ( Kaspari & Weiser, 1999 ) states that (1) as organisms decrease in size, they perceive their environment as being more rugose; (2) long legs allow organisms to step over obstacles but hinder them from entering small gaps; and (3) as the size of an organism decreases, the benefits of long legs begin to be outweighed by the costs of construction. Natural selection should therefore favour proportionally longer legs in larger organisms, thereby leading to a positive allometry between leg and body length (scaling exponent b > 1).
• 2 Here we compare the scaling exponent of leg‐to‐body length relationships among insects that walk, walk and fly, and predominantly fly. We measured the lengths of the hind tibia, hind femur, and body length of each species.
• 3 The taxa varied considerably in the scaling exponent b. In seven out of ten groups (Formicidae, Isoptera, Carabidae, Pentatomidae, Apidae, Lepidoptera, Odonata adult), b was significantly greater than one. However, there was no gradual decrease in b from walking to walking/flying to flying insects.
• 4 The results of the present study provide no support for the size–grain hypothesis. We propose that leg length is not only affected by the rugosity of the environment, but also by (1) functional adaptations, (2) phylogeny, (3) lifestyle, (4) the type of insect development (hemimetabolism or holometabolism), and (5) constraints of gas exchange.

     

Random patch dynamics of larval Chironomidae (Diptera) in the bed sediments of a gravel stream

• 1 The larval chironomid community of the sediment surface and the hyporheic inters titial was investigated in two longitudinal transects of an alpine gravel stream between September 1984 and August 1985. Eighty larval species and species groups were identified, most of which belonged to the subfamily Orthocladiinae. Of all larval individuals 51.1% inhabited the first 10cm of the bed sediments, and 93.2% occurred between the surface and 40cm depth.
• 2 The spatial species turnover showed marked variations between horizontally adjacent sampling sites in each of the four sediment depth layers. In both transects the species composition showed a significantly lower turnover in the upper 10cm of the bed sediments than in the deeper layers.
• 3 Spatial community stability showed an oscillating pattern between all sampling sites due to density shifts of larvae between depth layers. Temporal differences in resilience (local stability) were significantly and positively related to changes in the cumulative discharge pattern in the gravel brook, thus indicating the apparent ability of the community to recover quickly following disturbances.
• 4 The five abundant species, Corynoneura lobata, Synorthocladius semivirens, Tvetenia calvescens, Micropsectra atrofasciata and Rheotanytarsus nigricauda, exhibited significant differences in their sediment depth distribution, with density maxima shifting between depth layers. Spatial autocorrelations suggest that these larvae form patches between neighbouring sampling sites with varying sizes and inter-patch distances in each of four sediment layers. A simulation test, in which individuals of each species were randomly permuted between microhabitats of each depth layer separately, indicated that the patches might have arisen by chance.
• 5 To evaluate the significance of observed spatial resource overlap values amongst these five chironomid species, neutral models were developed based on 300 randomizations of each possible species pair-wise association of individuals and patches of species. The spatial organization of a larval chironomid assemblage in the stream Oberer Seebach seemed to be governed by coexistence due to random patch formation and dispersal patterns within the interstitial habitats, which reduce the probability of strong competitive interactions.

     

Patterns of resource distribution among conspecific larvae in two fruit fly species: Anastrepha fraterculus and Ceratitis capitata (Diptera: Tephritidae)

相似文献   

Studies on Chironomidae in experimental recirculating stream systems. II. The growth, development and production of a spring generation of Orthocladius (Euorthodadius) calvus Pinder

SUMMARY.

• 1 Orthodadius (Euorthodadius) calvus Pinder. similar to Orthocladius (Euorthodadius) thienemanni Kieffer. colonized a new gravel substratum in two recirculating stream channels. A maximum population density of 68.621 m^-2 was attained after only 16 days. This had fallen to a negligible density by the thirty-third day.
• 2 Some recruitment occurred over most of the study period (April-May 1981) and no single sharply defined cohort was evident. There was a large range of body lengths within each instar. and considerable overlap between instars. The population density estimates for instars I and II were low compared with instars III and IV.
• 3 The relationship between instantaneous growth rate (g) and geometric mean length indicated that growth was best described by a Gompertz curve. Growth rate decreased with increasing length from a value of about 40% length day^-1 at 2 mm body length to about 5% length day^-1 at 9 mm.
• 4 Growth rates for individual larvae, kept in culture, were very variable with maximum rates close to the values determined from the field data. Mean duration of larval life was 16 days.
• 5 Estimates of production for the study period ranged from 13.5 g dry wt m^-2 (Channel III, size-frequency method) to 34.2 g dry wt m^-2 (Channel II, Allen's graphical method, values corrected for non-linear growth).
• 6 Gut contents were estimated to represent about 55% of the total weight therefore production values should be reduced by this amount.

     

Distribution,abundance and diversity of macrozoobenthos in Aiba Reservoir,Iwo, Nigeria

Spatial and seasonal variation in macrozoobenthic composition, abundance and diversity in Aiba Reservoir were investigated bimonthy between June 2004 and April 2005 using a van Veen grab. A depauperate fauna of nine taxa was recorded. Generally, larger numbers of taxa were recorded during the dry season than in the wet season. Melanoides tuberculata and chironomid larvae dominated the macrozoobenthos and showed wide spatial distribution. Diversity and evenness were lower during the wet season than in the dry season, when densities of the major species were low. Melanoides tuberculata and chironomid larvae were recorded from maximum depths of 3.9 m and 2.6 m, respectively. Both taxa were also recorded from all substrate texture types, regardless of the amount of macrophyte material present. The benthic fauna is characterised as being poor in terms of density, taxonomic richness and diversity     

Length-mass relationships of macro-invertebrates in a freshwater stream in Japan

Relationships between weight (W; dry weight) and length (L; head capsule width or total body length) were examined in 25 freshwater macro-invertebrates at species level and four at order level using the formula W = aL^b. Our estimates at the order level dropped into the range of those in North America, Europe, New Zealand, and previous studies in Japan with the exception of Trichoptera. Differences encountered in Trichoptera may be due to high interspecific variation in length-mass relationships and size-range of component taxa. In addition, our results show that the relationships could differ at the genus level. Intrageneric morphological variations related to microhabitat differences have been reported; careful estimates may thus be needed for the length-mass relationships even in the same genus if the targets have diverse ecological traits.     

Cube law, condition factor and weight–length relationships: history, meta-analysis and recommendations

This study presents a historical review, a meta‐analysis, and recommendations for users about weight–length relationships, condition factors and relative weight equations. The historical review traces the developments of the respective concepts. The meta‐analysis explores 3929 weight–length relationships of the type W = aL^b for 1773 species of fishes. It shows that 82% of the variance in a plot of log a over b can be explained by allometric versus isometric growth patterns and by different body shapes of the respective species. Across species median b = 3.03 is significantly larger than 3.0, thus indicating a tendency towards slightly positive‐allometric growth (increase in relative body thickness or plumpness) in most fishes. The expected range of 2.5 < b < 3.5 is confirmed. Mean estimates of b outside this range are often based on only one or two weight–length relationships per species. However, true cases of strong allometric growth do exist and three examples are given. Within species, a plot of log a vs b can be used to detect outliers in weight–length relationships. An equation to calculate mean condition factors from weight–length relationships is given as K_mean = 100aL^b?3. Relative weight W_rm = 100W/(a_mL^bm) can be used for comparing the condition of individuals across populations, where a_m is the geometric mean of a and b_m is the mean of b across all available weight–length relationships for a given species. Twelve recommendations for proper use and presentation of weight–length relationships, condition factors and relative weight are given.     

Complex relations between freshwater macro- and meiobenthos: interactions between Chironomus riparius and Chydorus piger

• 1 The potential importance of interactions between macro- and meiobenthos in the littoral zone of takes was studied in a series of laboratory experiments with a chironomid and a chydorid.
• 2 In a situation of intraspecific density dependence, Chydorus piger Sars inhibited the growth of Chironomus riparius Meigen, while both second- and fourth-instar C, riparius stimulated the parthenogenetic reproduction of C. piger.
• 3 The meiofaunal chydorids may thus have a structuring effect on macrofaunal chironomid populations by interfering with the early-instar larvae.
• 4 With macro- and meiofaunal species both operating at the primary consumer-level of the food web, this kind of complex interaction will affect the magnitude of secondary production available to higher trophic levels.

     

Sharing morphospaces: early ontogenetic shape changes in two clingfish larvae (Pisces: Gobiesocidae) from the south‐east Pacific Ocean

Larval body shape changes and developmental timing were examined in two clingfish species from the south‐east Pacific Ocean, Gobiesox marmoratus and Sicyases sanguineus. Ontogenetic allometry showed no interspecific variation and <7 mm standard length (L_S) larvae of both species occupied similar morphospace, but larger G. marmoratus showed increased body depth while larvae of S. sanguineus developed a flattened head and maintained a hydrodynamic body. Estimated developmental timing suggests that larval body shape changes were faster in G. marmoratus than in S. sanguineus prior to settlement.     

Solar radiation directly affects larval performance of a forest insect

相似文献