底栖藻类对消落带土壤中各形态磷的影响

为揭示底栖藻类对三峡库区消落带淹没初期土壤中不同形态磷的影响作用,开展了相关的模拟实验。结果表明,在模拟的底栖藻类影响条件下,消落带土壤中铝磷（Al-P）的含量在实验期间处于波动状态,Al-P相对于其余形态的磷更易于被藻类吸收利用,且与藻类生长存在密切相关,即底栖藻类生长状态较好时,土壤中Al-P呈现下降趋势;而生长状态较差时,土壤中Al-P又出现略微上升的趋势。在实验后期土壤中Al-P含量上升,可能是其余形态的磷转化而来。底栖藻类的生长对不同深度土壤中铁磷（Fe-P）的影响不同,其生长对2-4 cm层土壤的影响较大,而对其余层影响不大。不同深度土壤中闭蓄态磷（O-P）和钙磷（Ca-P）含量差异不明显。实验后期Fe-P、O-P和Ca-P含量一直呈现下降的趋势,只有Al-P在后期出现上升的趋势,说明在淹水条件和底栖藻类生物膜共同作用下,土壤磷的有效性提高,土壤中难溶性磷向易溶性磷转化。       

Effect of algal growth on the availability of phosphorus,iron and manganese in rice soils

Summary Laboratory experiments were conducted to study the effect of algal growth on the change of (I) pH, (II) available phosphorus and (III) solubility of iron and manganese content in five waterlogged alluvial rice soils of West Bengal, India. The results showed that the algal growth initially caused an increase in the soil pH, which later declined to the original value in some of the soils. The available phosphorus content decreased upto 90 days of their growth and began to increase towards the later period of incubation. The drastic fall of water soluble plus exchaneable manganese content of the soils due to algal growth was accompanied by similar increase in reducible manganese content. No appreciable change in water soluble plus exchangeable ferrous iron content was encountered but theN-NH₄OAC(pH 3) extractable iron due to algal growth progressively decreased with the progress of the incubation period.     

A greenhouse study on the effect of inoculation of N-fixing blue-green algae in an alluvial soil treated with P and Mo on the yield of rice and changes in the N-content of soil

Summary The efficiency of the inoculation of three cultures of N-fixing blue-green algaeviz. (i)Aulosira fertilissima (A₁), (ii)Nostoc muscorum (A₂) and (iii) their mixture (A₃) in increasing the grain and straw yield of rice, nitrogen uptake in grain and nitrogen content in soil was studied in a green house experiment with an alluvial soil in presence or absence of urea nitrogen application. Inoculation significantly increased the grain and straw yield of rice and nitrogen uptake in grain, but the efficiency of inoculation gradually decreased with the increase in the levels of urea nitrogen application, the extent of decrease varying with the algal cultures inoculated. The nitrogen content in the soils after the crop harvest recorded a significant increase due to inoculation but after air drying the soil a marker decrease of the same was observed, which indicated that most of the nitrogen added to the soil by blue-green algae through fixation did not persist after air drying the soil.     

Soil transfers from valley oak (Quercus lobata Nee) stands increase ectomycorrhizal diversity and alter root and shoot growth on valley oak seedlings

 Soils from valley oak (Quercus lobata Nee) riparian areas of the Cosumnes River Nature Conservancy Preserve near Sacramento, California were added to growth medium of valley oak seedlings grown in a greenhouse or in agricultural fields at Cosumnes which probably once supported valley oak trees and are now replanted with native riparian vegetation or allowed to revegetate naturally. Agricultural field soil from the Cosumnes River Preserve was presumed to be low or lacking in ectomycorrhizal inoculum. The study was designed to (1) determine whether valley oak stand soil transfer could cause mycorrhizal infection on valley oak seedlings in an agricultural field and in a greenhouse, (2) describe ectomycorrhizal morphological types formed on valley oak seedlings, and (3) determine whether seedling growth is enhanced more by transfer of natural valley oak stand soil than agricultural field soil. In the field study, transfer of forest soil increased average ectomycorrhizal diversity (2.4 types) more than transfer of agricultural field soil (1.2 types). Valley oak seedlings were responsive to ectomycorrhizal infection in the field study. With increase in mycorrhizal infection there was an increase in shoot growth at the expense of root growth. In the greenhouse study, both percent mycorrhizal infection and mycorrhizal diversity were increased more by transfer of oak forest and woodland soils than agricultural field soil. Eight morphotypes occurred on seedlings in forest and woodland soils but only three morphotypes in agricultural soil. This result strongly suggests that the agricultural field also harbors ectomycorrhizal propagules but forest and woodland soils support a more abundant and diverse ectomycorrhizal flora. Accepted: 17 August 1997     

Screening,production, optimization and characterization of cyanobacterial polysaccharide

Biotechnological applications of algal polysaccharide as emulsifiers, thickeners and laxatives have led to the screening and selection of certain diazotrophic filamentous cyanobacteria from saline/alkaline soil of Madhya Pradesh, India. Strain specific variation in cell bound, extracellular and total polysaccharide content was quantified under laboratory conditions. Among the cyanobacterial isolates examined Nostoc calcicola RDU-3 was found to produce highest amount (105 mg l⁻¹) of extracellular polysaccharide on 44th day of growth under diazotrophic growth conditions. Extracellular polysaccharide production of cyanobacterium Nostoc calcicola RDU-3 was optimal at pH-10, temperature 35°C, photoperiod of 24 h and in white light. The Gas Chromatographic analysis of polysaccharide from Nostoc calcicola RDU-3 revealed the presence of ribose (36.03%), xylose (34.13%), rhamnose (29.67%) and glucose (4.0%). The polysaccharide is novel in that it possesses ribose as the predominant monosaccharide with very low levels of glucose. Predominance of ribose monosaccharide is the unique feature which is reported to be used as metabolic supplement to the heart. IR spectrum of extracellular polysaccharide revealed the presence of sulphate group. Such sulphated polysaccharide is reported to have antiviral properties.     

Effect of Interactions Among Algae on Nitrogen Fixation by Blue-Green Algae (Cyanobacteria) in Flooded Soils

Nitrogen fixation (C₂H₂ reduction) by algae in flooded soil was limited by interactions within the algal community. Nitrogen fixation by either indigenous algae or Tolypothrix tenuis was reduced severalfold by a dense suspension of the green alga Nephrocytium sp. Similarly, interactions between the nitrogen-fixing alga (cyanobacterium) Aulosira 68 and natural densities of indigenous algae limited nitrogen-fixing activity in one of two soils examined. This was demonstrated by developing a variant of Aulosira 68 that was resistant to the herbicide simetryne at concentrations that prevented development of indigenous algae. More nitrogen was fixed by the resistant variant in flooded soil containing herbicide than was fixed in herbicide-free soil by either the indigenous algae or indigenous algae plus the parent strain of Aulosira. Interference from indigenous algae may hamper the development of nitrogen-fixing algae introduced into rice fields in attempts to increase biological nitrogen fixation.     

Food preference,food quality and diets of three herbivorous gastropods (Trochidae: Tegula) in a temperate kelp forest habitat

Summary The relationship between food preference and food quality (i.e. a food's contribution to growth and reproductive development) was examined in the laboratory for 3 species of herbivorous kelp forest gastropods (Tegula). The preference hierarchies of the 3 Tegula for 6 common algal species are the same: giant kelp (Macrocystis) is the most preferred food and brown algae are consumed at higher rates than red algae. Despite their strong preference for Macrocystis, the 3 species had significantly greater growth and/or reproductive development on a mixed-algae diet than on either brown or red algae alone. Laboratory preferences of the snails did not correspond closely with caloric content, estimated availability or quality of the algal species used in this study. However, the 3 Tegula are subject to strong benthic predation and Macrocystis provides an important spatial refuge in nature. The potential role of non-nutritional factors such as predator avoidance on the formation of food preferences is discussed.     

STUDIES OF HAWAIIAN FRESHWATER AND SOIL ALGAE II. ALGAL COLONIZATION AND SUCCESSION ON A DATED VOLCANIC SUBSTRATE1,2

Spatial studies of colonization and succession of soil algae and chemical analyses of the various soils on the cinde cone of Kilauea Iki in Hawaii Volcanoes National Park, Hawaii are outlined. There is a positive correlation between the diversity and quantity of soil algae with nutrient levels and organic matter accumulation in each locale. Three distinct edaphic-biotic zones existing in this area are differentially revealed by the soil chemical composition, quantity and diversity of soil algae, and as evident variations in higher plant growth and colonization. Varying colonization and successional phases of higher plant growth around standing and fallen tree snags killed by volcanic activity also reflect variations in the soil algal flora. These variations appear largely as a function of differential water interception, absorption, and retention as well as differential accumulation of organic matter, and the initiation of various biogeochemical cycles.     

Influence of draining on soil phosphorus forms and distribution in a constructed wetland

Seasonal water-table fluctuations in wetlands can result in flooded and drained conditions in the surface soil. In constructed wetlands water level drawdown and soil drainage are used in management to consolidate detrital materials, accelerate soil build up, and provide easy access for other management operations. A greenhouse study was conducted using intact peat soil cores to evaluate the changes in bioavailable P and other fractions following draining and reflooding. Measurements of floodwater dissolved reactive P (DRP) indicated that draining and soil exposure could result in large P flux to the overlying water column. Phosphorus flux in soils drained for 6 weeks was 10-fold higher (334 mg P m⁻² day⁻¹) than in soils drained for 3 weeks (33 mg P m⁻² day⁻¹). Soil exposure also resulted in an increase in bioavailable inorganic P (estimated by KCl extraction) at the expense of labile organic P pool. The KCl-P pool, which was initially less than 2% of total P (TP), increased to 3% and 13% of TP after 3 and 6 weeks draining, respectively. Results suggest that various soil P fractions, particularly those in newly accreted materials, were highly unstable and could be released in a more available form when newly accreted soils undergo drying. Water level drawdown and reflooding could result in significant P release, a possible stimulation of algal blooms and other water quality problems. Therefore, soil characteristics and chemistry and their impact on water quality should be a major consideration when one adopts the flood-drain technique in wetland management.     

Green algae in tundra soils affected by coal mine pollutions

Green algal communities were investigated in clean and pollution-impacted tundra soils around the large coal mine industrial complex of Vorkuta in the E. European Russian tundra. Samples were collected in three zones of open-cast coal mining with different degrees of pollution-impacted soil transformation. A total of 42 species of algae were found in all zones. The species richness decreased from 27 species in undisturbed zones to 19 species in polluted zones. Under open-cast coal mining impacts the community structure simplified, and the dominant algae complexes changed. Algae that are typical for clean soils disappeared from the communities. The total abundance of green algae (counted together with Xanthophyta) ranged between 100–120 × 10³ (cells/g dry soils) in undisturbed zones and 0.5–50 × 10³ in polluted zones. Soil algae appear to be better indicators of coal mine technogenic pollution than flowering plants and mosses. Presented at the International Symposium Biology and Taxonomy of Green Algae V, Smolenice, June 26–29, 2007, Slovakia.     

Recycling of manure nutrients: use of algal biomass from dairy manure treatment as a slow release fertilizer

An alternative to land spreading of manure is to grow crops of algae on the N and P present in the manure and convert manure N and P into algal biomass. The objective of this study was to evaluate the fertilizer value of dried algal biomass that had been grown using anaerobically digested dairy manure. Results from a flask study using two soils amended with algal biomass showed that 3% of total algal nitrogen (N) was present as plant available N at day 0. Approximately 33% of algal N was converted to plant available N within 21 days at 25 degrees C in both soils. Levels of Mehlich-3 extractable phosphorus (P) in the two soils rose with increasing levels of algal amendment but were also influenced by existing soil P levels. Results from plant growth experiments showed that 20-day old cucumber and corn seedlings grown in algae-amended potting mix contained 15-20% of applied N, 46-60% of available N, and 38-60% of the applied P. Seedlings grown in algae-amended potting mixes were equivalent to those grown with comparable levels of fertilizer amended potting mixes with respect to seedling dry weight and nutrient content. These results suggest that dried algal biomass produced from treatment of anaerobically digested dairy manure can substitute for commercial fertilizers used for potting systems.     

Biological,chemical and physical factors influencing the seasonal change of polysaccharide concentration in irrigation-channel sediment

Variation of polysaccharide concentration in irrigation-channel sediment was determined concurrently with biological, chemical and physical factors influencing the benthic algal community. Phenol-sulphuric acid method was used to measure polysaccharide concentration. Polysaccharide concentration, biomass of benthic algae, and species composition changed spatially and temporally. Fluctuations of total suspended solid (TSS) concentration and exposure of channel bed to direct sunlight had major effects on algal growth and polysaccharide production. Polysaccharide concentration was correlated to chlorophyll a concentration (r=0.73, P<0.001) and algal biomass (r=0.57, P<0.001). Fragilaria construens and Aulacoseira (Melosira) italica were the most common diatoms in the benthic flora. Chlorophyll a concentration in the sediment showed a strong negative correlation (r=-0.99, P<0.001) with the seasonal variation of TSS concentration in channel water. The polysaccharides produced by benthic microorganisms play a major role in clogging channel bed and thereby reducing seepage from earthen irrigation channels. Correlations between polysaccharide concentration and chlorophyll a (and algal biomass) further indicate the importance of benthic algae for polysaccharide production. Since availability of light to the algal flora is critical for the production of polysaccharides, the effect of clogging can be maximized by exposing the channel bed to direct sunlight during non-irrigation period (winter).     

Effect of soil moisture and phosphate level on root hair growth of corn roots

Summary Root hairs have been shown to enhance P uptake by plants growing in low P soil. Little is known of the factors controlling root hair growth. The objective of this study was to investigate the influence of soil moisture and P level on root hair growth of corn (Zea mays L.). The effect of volumetric soil moistures of 22% (M₀), 27% (M₁), and 32% (M₂) and soil (Raub silt loam, Aquic Argiudoll) P levels of, 0.81 (P₀), 12.1 (P₁), 21.6 (P₂), 48.7 (P₃), and 203.3 (P₄) mol P L^–1 initially in the soil solution, on shoot and root growth, P uptake, and root hair growth of corn was studied in a series of pot experiments in a controlled climate chamber. Root hair growth was affected more by soil moisture than soil P. The percentage of total root length with root hairs and the density and length of root hairs on the root sections having root hairs all increased as soil moisture was reduced from M₂ to M₀. No relationship was found between root hair length and soil P. Density of root hairs, however, was found to decrease with an increase in soil P. No correlation was found between root hair growth parameters and plant P content, further suggesting P plays a secondary role to moisture in regulating root hair growth in soils. The increase in root hair growth appears to be a response by the plant to stress as yield and P uptake by corn grown at M₀ were only 0.47 to 0.82, and 0.34 to 0.74, respectively, of that measured at M₁ across the five soil P levels. The increase in root hair growth at M₀, which represents an increase of 2.76 to 4.03 in root surface area, could offset, in part, the reduced rate of root growth, which was the primary reason for reduced P uptake under limited soil moisture conditions.Journal Paper No. 10,066 Purdue Univ. Agric. Exp. Stn., W. Lafayette, IN 47907. Contribution from the Dep. of Agron. This paper was supported in part by a grant from the Tennessee Valley Authority.     

PRODUCTION AND NUTRIENT REMOVAL BY PERIPHYTON GROWN UNDER DIFFERENT LOADING RATES OF ANAEROBICALLY DIGESTED FLUSHED DAIRY MANURE1

Growing algae to scrub nutrients from manure presents an alternative to the current practice of land application and provides utilizable algal biomass as an end product. The objective of this study was to assess algal growth, nutrient removal, and nitrification using higher light intensities and manure loading rates than in the previous experiments. Algal turfs, with periphyton mainly composed of green algal species, were grown under two light regimes (270 and 390 μmol photons·m^?2· s^?1) and anaerobically digested flushed dairy manure wastewater (ADFDMW) loading rates ranging from 0.8 to 3.7 g total N and 0.12 to 0.58 g total P·m^?2·d^?1. Filamentous cyanobacteria (Oscillatoria spp.) and diatoms (Navicula, Nitzschia, and Cyclotella sp.) partially replaced the filamentous green algae at relatively high ADFDMW loading rates and more prominently under low incident light. Mean algal production increased with loading rate and irradiance from 7.6±2.71 to 19.1±2.73 g dry weight· m^?2·d^?1. The N and P content of algal biomass generally increased with loading rate and ranged from 2.9%–7.3% and 0.5%–1.3% (by weight), respectively. Carbon content remained relatively constant at all loading rates (42%–47%). The maximum removal rates of N and P per unit algal biomass were 70 and 13 mg·g^?1 dry weight·m^?2·d^?1, respectively. Recovery of nutrients in harvested algal biomass accounted for about 31%–52% for N and 30%–59% for P. Recovery of P appeared to be uncoupled with N at higher loading rates, suggesting that algal potential for accumulation of P may have already been saturated. It appears that higher irradiance level enhancing algal growth was the overriding factor in controlling nitrification in the algal turf scrubber units.     

The toxicity of phenolic compounds to soil algal population and toChlorella vulgaris andNostoc linckia

Summary p-Nitrophenol (PNP),m-nitrophenol (MNP), 2,4-dinitrophenol (DNP) and catechol were tested for their effects on algal population in a soil and on pure cultures of two algae isolated from soil. Both PNP and MNP, even at 0.5 kg ha⁻¹ level were toxic to the soil algae; high doses effected increase in toxicity. Inhibition of algae was relatively more with PNP compared to the other two nitrophenols. Catechol treatment up to 1.0 kg ha⁻¹ led to a significant initial enhancement of algae with a subsequent far less toxic effect. The toxicity of the phenolic compounds towardChlorella vulgaris, a green alga andNostoc linckia, a blue-green alga, decreased in the order: MNP≧PNP>DNP>Catechol. However, algicidal or algistatic effect of the test chemicals was fairly more againstC. vulgaris, suggesting that the eukaryotic alga is highly sensitive to such soil pollutants compared to the prokaryotic alga.     

Effect of periodate oxidation on the polysaccharide content and microaggregate stability of rhizosphere and non-rhizosphere soils

Summary The relationship between the stability of soil microaggregates in water and the polysaccharide content was examined in rhizosphere and non-rhizosphere samples from a pot experiment using three soils that had grown peas, barley or grasses. The polysaccharide was oxidised and removed using 168h treatment with 0.02M periodate followed by 6h with 0.1M tetraborate. The decrease in polysaccharide content, measured as change in residual reducing sugars, was compared with the stability of soil microaggregates (ca 45m) in water, determined by a turbidimetric method.Total C, N and polysaccharide contents of rhizosphere soils were greater than those for the bulk soil, but the water stability of aggregates was not increased compared to unplanted controls. Periodate oxidation removed a large proportion (59–95%) of the polysaccharide and increased aggregated disruption, but there was no clear relationship between the two measurements. In rhizosphere soil, polysaccharides appreared to make less contribution to aggregate stability than polysaccharide in the bulk soil. The relatively small effect of rhizosphere polysaccharides is probably related to their presence as comparatively massive plant remains and debris; this contrasts with the decomposed and transformed material in the bulk soil.     

Stoichiometric impacts of increased carbon dioxide on a planktonic herbivore

The partial pressure of carbon dioxide (pCO₂) in lake ecosystems varies over four orders of magnitude and is affected by local and global environmental perturbations associated with both natural and anthropogenic processes. Little is known, however, about how changes in pCO₂ extend into the function and structure of food webs in freshwater ecosystems. To fill this gap, we performed laboratory experiments using the ecologically important planktonic herbivore Daphnia and its algal prey under a natural range of pCO₂ with low light and phosphorus supplies. The experiment showed that increased pCO₂ stimulated algal growth but reduced algal P : C ratio. When feeding on algae grown under high pCO₂, herbivore growth decreased regardless of algal abundance. Thus, high CO₂‐raised algae were poor food for Daphnia. Short‐term experimental supplementation of PO₄ raised the P content of the high CO₂‐raised algae and improved Daphnia growth, indicating that low Daphnia growth rates under high pCO₂ conditions were due to lowered P content in the algal food. These results suggest that, in freshwater ecosystems with low nutrient supplies, natural processes as well as anthropogenic perturbations resulting in increased pCO₂ enhance algal production but reduce energy and mass transfer efficiency to herbivores by decreasing algal nutritional quality.     

Soil changes accompanying invasion of the exotic shrub Cytisus scoparius in glacial outwash prairies of western Washington [USA]

Shrub encroachment of grassland is a global phenomenon that can cause substantial and rapid changes in soil nutrient levels and distribution. If the woody plants in question also have the capacity to fix nitrogen (N), the effects on soil nutrients may potentially be large. Cytisus scoparius L. Link (Scotch broom) is an introduced leguminous shrub from the Mediterranean region that colonizes open grasslands. We measured several properties of the surface soil (0-10 cm depth) across advancing fronts of Cytisus to assess changes in soil fertility associated with invasion in western Washington state (USA). Both total soil C and N increased (1.8% and 6.5%, respectively), resulting in a decrease in C to N ratio of 22.5 in uninvaded soils to 21.5 inside Cytisus patches. δ¹⁵N signature of surface soil did not clearly demonstrate a signal of N₂-fixation across the Cytisus gradient. On the other hand, seven-day laboratory aerobic incubations demonstrated 3 and 2.4 times greater mineralization and nitrification rate, respectively, in Cytisus soils compared to uninvaded soils. Despite this increase in N availability, bioassay plants (Achillea millefolium) grown in uninvaded soils in the greenhouse were ∼ 30% larger than those grown in Cytisus-invaded soils, suggesting that Cytisus may have inhibitory effects on some plants growing in invaded soils. These results suggest that the impact of Cytisus invasion on grassland plant communities may be influenced or tempered by chemical or microbial effects on the soil other than simply increased labile N. This revised version was published online in June 2006 with corrections to the Cover Date.     

Improvement of edaphic cyanobacterial population through liming in red and lateritic rice soils of West Bengal

In view of the predominance of acidic pH levels in lateritic soils and of the importance of this soil property on growth and distribution of blue-green algae or cyanobacteria (CB), the effect of lime application on the reduction of acidity and subsequently on growth and some related physiological properties of CB have been studied. Four soils with varying pH levels were used for this purpose and three lime treatments corresponding to 0,50 and 100% lime requirement were applied to each of them. The soils thus treated were incubated under submerged condition in an illuminated chamber for 6 weeks and were drawn at weekly intervals for studies of the number, fresh mass and chlorophyll content of the developed algal mass. Increase of the pH value of the soil through application of lime improved growth and relevant physiological properties of the CB.     

Effect of Watering and Soil Moisture on Mercury Emissions from Soils

This paper presents data from experiments that measured Mercury (Hg) flux as a function of water addition and subsequent soil drying, and maintenance of soil water content over time utilizing small dynamic gas exchange chambers and large mesocosms. When soil surfaces were dry and water was added at an amount less than that necessary to saturate the soil an immediate large (relative to dry soil flux) release of Hg occurred. Diel Hg emissions from soils, unenriched (0.02 μg g⁻¹) and enriched (3 μg g⁻¹) in Hg and wet below saturation, were significantly elevated above that occurring from dry soils (2–5 times depending on soil water content) for weeks to months. Enhancement of emissions from wet soils in direct sunlight were greater than that from soils shaded or in the dark suggesting that a synergism exists between soil moisture and light. When soils were watered to saturation Hg emissions were suppressed or remained the same depending on the degree of saturation. It is hypothesized that the addition of soil water in amounts less than that necessary to saturate the soil surface results in an immediate release of elemental Hg from soil surface as the more polar water molecule out competes Hg for binding sites. As the water moves into the soil, Hg adsorbed to soil particles is desorbed into soil gas and dissolved in the soil water. The process of evaporation facilitates movement of Hg as mass flow to the soil surface where it is made available for subsequent release. The latter is hypothesized to be an important process by which Hg is recharged at the soil–air interface.