共查询到20条相似文献,搜索用时 15 毫秒
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Margaretha van der Deen Hanna Taipaleenm?ki Ying Zhang Nadiya M. Teplyuk Anurag Gupta Senthilkumar Cinghu Kristen Shogren Avudaiappan Maran Michael J. Yaszemski Ling Ling Simon M. Cool David T. Leong Christian Dierkes Jozef Zustin Manuel Salto-Tellez Yoshiaki Ito Suk-Chul Bae Maria Zielenska Jeremy A. Squire Jane B. Lian Janet L. Stein Gerard P. Zambetti Stephen N. Jones Mario Galindo Eric Hesse Gary S. Stein Andre J. van Wijnen 《The Journal of biological chemistry》2013,288(29):21307-21319
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Toshihisa Komori 《Cell and tissue research》2010,339(1):189-195
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Francisco Villanueva Hector Araya Pedro Briceño Nelson Varela Andres Stevenson Sofia Jerez Fabian Tempio Jonas Chnaiderman Carola Perez Milena Villarroel Emma Concha Farzaneh Khani Roman Thaler Flavio Salazar-Onfray Gary S Stein Andre J. van Wijnen Mario Galindo 《Journal of cellular physiology》2019,234(8):13659-13679
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S Sudhakar Y Li M S Katz N Elango 《Biochemical and biophysical research communications》2001,289(2):616-622
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Tomé M López-Romero P Albo C Sepúlveda JC Fernández-Gutiérrez B Dopazo A Bernad A González MA 《Cell death and differentiation》2011,18(6):985-995
In spite of the extensive potential of human mesenchymal stem cells (hMSCs) in cell therapy, little is known about the molecular mechanisms that regulate their therapeutic properties. We aimed to identify microRNAs (miRNAs) involved in controlling the transition between the resting and reparative phenotypes of hMSCs, hypothesizing that these miRNAs must be present in the undifferentiated cells and downregulated to allow initiation of distinct activation/differentiation programs. Differential miRNA expression analyses revealed that miR-335 is significantly downregulated upon hMSC differentiation. In addition, hMSCs derived from a variety of tissues express miR-335 at a higher level than human skin fibroblasts, and overexpression of miR-335 in hMSCs inhibited their proliferation and migration, as well as their osteogenic and adipogenic potential. Expression of miR-335 in hMSCs was upregulated by the canonical Wnt signaling pathway, a positive regulator of MSC self-renewal, and downregulated by interferon-γ (IFN-γ), a pro-inflammatory cytokine that has an important role in activating the immunomodulatory properties of hMSCs. Differential gene expression analyses, in combination with computational searches, defined a cluster of 62 putative target genes for miR-335 in hMSCs. Western blot and 3'UTR reporter assays confirmed RUNX2 as a direct target of miR-335 in hMSCs. These results strongly suggest that miR-335 downregulation is critical for the acquisition of reparative MSC phenotypes. 相似文献