柔嫩艾美耳球虫感染对鸡盲肠中益生菌的影响

双歧杆菌（Ｂｉｆｉｄｏｂａｃｔｅｒｉｕｍｓｐｐ．）及乳酸杆菌（Ｌａｃｔｏｂａｃｉｌｌｕｓｓｐｐ．）被认为是动物机体内重要的有益微生物菌群之一。本文对鸡感染柔嫩艾美耳球虫后盲肠中上述两种微生物的变化情况进行了研究。结果表明：鸡在感染球虫后第４、７、１０ｄ时盲肠中双歧杆菌的数量显著减少（Ｈ＜０．０５）;在球虫感染后的第４、７、１４ｄ时,盲肠中的乳酸杆菌呈显著下降趋势（Ｐ＜０．０５）。     

柔嫩艾美耳球虫感染对鸡盲肠中益生菌的影响*

双歧杆菌（Ｂｉｆｉｄｏｂａｃｔｅｒｉｕｍｓｐｐ．）及乳酸杆菌（Ｌａｃｔｏｂａｃｉｌｌｕｓｓｐｐ．）被认为是动物机体内重要的有益微生物菌群之一。本文对鸡感染柔嫩艾美耳球虫后盲肠中上述两种微生物的变化情况进行了研究。结果表明：鸡在感染球虫后第４、７、１０ｄ时盲肠中双歧杆菌的数量显著减少（Ｈ＜０．０５）;在球虫感染后的第４、７、１４ｄ时,盲肠中的乳酸杆菌呈显著下降趋势（Ｐ＜０．０５）。     

不同海拔高度川滇高山栎群体遗传多样性的变化

采集海拔２２００～３８００ｍ川滇高山栎（ＱｕｅｒｃｕｓａｑｕｉｆｏｌｉｏｉｄｅｓＲｅｈｄ．ｅｔＷｉｌｓ．）８个群体的样品,应用同工酶电泳的方法研究其遗传变异水平,并发现遗传变异水平随着海拔高度变化呈以下趋势：海拔２４００～３３００ｍ川滇高山栎的遗传变异水平较高,而海拔３８００ｍ及２２００ｍ群体的遗传变异水平均有降低的趋势。另外,还发现人为干扰、日照多少对遗传变异水平均有影响。     

吕梁山严村低中山区植物群落演替分析

采用一次性同时调查的方法获得数据,用数量分类（ＴＷＩＮＳＰＡＮ）和排序（ＤＣＡ）研究严村低中山植物群落演替。在黄土弃耕地上群落的演替顺序为：苦苣＋狗尾草群落（Ｃｏｍｍ．Ｉｘｅｒｉｓ ｃｈｉｎｅｎｓｉｓ ｓｓｐ．ｖｅｒｓｉｃｏｌｏｒ＋Ｓｅｔａｒｉａ ｖｉｒｉｄｉｓ）→蒿类群落（Ｃｏｍｍ．Ａｒｔｅｍｉｓｉａ ｓｐｐ．）→野艾蒿＋披碱草群落（Ｃｏｍｍ．Ａｒｔｅｍｉｓｉａ ｌａｖａｎｄｕｌａｅｆｏｌｉａ＋     

柴胡类药材的柴胡皂甙分析

柴胡类药材的柴胡皂甙分析李光慧罗燕燕王瑛袁昌齐王年鹤（北京临床药学研究所,北京１０００３５）（江苏省中国科学院植物研究所,南京２１００１４）ＡｎａｌｙｓｉｓｏｆｓａｉｋｏｓａｐｏｎｉｎｓｉｎｍｅｄｉｃｉｎａｌＢｕｐｌｅｕｒｕｍｓｐｐ．ＬｉＧｕａｎｇ...     

pp60c-src在血管平滑肌细胞内丝裂原活化蛋白激酶激活中的作用

观察ｐｐ６０ｃ－ｓｒｃ在血管紧张素Ⅱ（ＡｎｇⅡ）诱导血管平滑肌细胞（ＶＳＭＣｓ）内丝裂原活化蛋白激酶（ＭＡＰＫ）激活中的作用,以了解ＡｎｇⅡ促ＶＳＭＣｓ增殖的信号转导过程。将合成的反义ｃ－ｓｒｃ寡脱氧核苷酸（ｏｌｉｇｏｄｅｏｘｙｎｕｃｌｅ－ｏｔｉｄｅｓ,ＯＤＮｓ）以脂质体包裹转染培养的大鼠ＶＳＭＣｓ,用Ｗｅｓｔｅｒｎ印迹测得细胞裂解液中ｐｐ６０ｃ－ｓｒｃ含量明显下降,免疫沉淀方法测得ｐｐ６０ｃ－ｓ     

丝核菌菌丝融合群种类,寄生专化性及与温度的关系

丝核菌（Ｒｈｉｚｏｃｔｏｎｉａｓｐｐ．）是最重要的土传植物病原菌之一,分布世界各地。１９２１年Ｍａｔｓｕｍｏｔｏ用菌丝融合群（ＡｎａｓｔｏｍｏｓｉｓＧｒｏｕｐ,简称ＡＧ）的方法来划分多核立枯丝核菌（Ｒ．ｓｏｌａｎｉ）在生理上特异的菌株［６］,Ｐａｒ...     

利用ORF438启动子在链霉菌中表达透明颤菌血红蛋白

利用ＯＲＦ４３８启动子在链霉菌中表达透明颤菌血红蛋白崔风文杨胜利（中国科学院上海生物工程研究中心上海２００２３３）１９８８年,由原核的透明颤菌（Ｖｉｔｒｅｏｓｃｉｌａｓｐｐ．）克隆到血红蛋白基因（ｖｇｂ）［１］,其后Ｍａｇｎｏｌｏ等在天蓝链霉菌及变青...     

果胶菌加根瘤菌诱发油菜结瘤固氮研究新进展

果胶细菌（ｐｅｃｔｉｎ－ｌｙｔｉｃ ｂａｃｔｅｒｉａ）处理油菜幼根（二天苗龄,根长３ｃｍ）,同时接种紫云英根瘤菌突变菌株（Ｒｈｉｚｏｂｉｕｒｎ ｓｐｐ．ａｓｔｒａｇｌｕｓ）结瘤率为９６．８０％。根瘤石蜡包理切片观察,结果是从根瘤的外皮层到内皮层细胞均有细菌的分布。乙炔还原法测定出低固氮酶活性（７３．１０ｎｂ ＭＣ２Ｈ４／株／小时）。在植株全氮、干重和盆栽产量上都有不同程度的增加。     

桐柏——大别造山带苏家河群早奥陶世微体化石及其意义

根据发现的几丁虫Ｃｏｎｏｃｈｉｔｉｎａｓｐ．,Ｃ．？ｓｐ．,Ｄｅｓｍｏｃｈｉｔｉｎａｓｐ．,Ｄ．ｂｒｅｃｈｙｔａ,Ｌａｇｅｎｏｃｈｉｔｉ－ｎａｃｆ．ｏｂｅｌｉｇｉｓ,Ｌ．ｃｆ．ｅｓｔｈｏｎｉｃａ,Ｒｈａｂｄｏｃｈｉｔｉｎａｓｐ．,Ｃａｌｐｉｃｈｉｔｉｎａ？ｓｐ．和疑源类Ｌｏｐｈｏｓｐｈａｅｒｉ－ｄｉｕｍｓｐ．,Ｌｅｉｏｓｐｈａｅｒｉｄｉａｓｐｐ．,Ｍｉｃｒｈｙｔｒｉｄｉｕｍｓｐ．,？Ａｃａｎｔｈｏｄｉａｅｒｏｄｉｕｍｓｐ．,Ｂａｌｔｉｓｐｈａｅｒｉ－ｄｉｕｍｓｐｐ．等微体化石确定河南信阳西双河地区苏家河群浒湾组为早奥陶世。它是桐柏－大别造山带（河南部分）第一次发现的有可靠古生物证据的奥陶纪地层。同时为该造山带区域地层序列的建立积累了资料,并为与秦岭造山带的衔接提供了新线索。此外还指出浒湾组的几丁虫生物群属于华南古生物地理区。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.