共查询到20条相似文献,搜索用时 27 毫秒
1.
A. V. Odgaeva V. B. Turovetskii A. A. Kamensky 《Moscow University Biological Sciences Bulletin》2007,62(4):156-157
Using the microfluorescent method of identification of damaged plasmatic membranes, the influence of hydrogen peroxide on mice peritoneal macrophages was determined. H2O2 in vitro produced a destructive effect on the cells depending on the temperature, time of incubation, and concentration of the agent. The damage increases after the incubation medium is replaced with Hanks solution containing no H2O2. 相似文献
2.
V. D. Samuilov L. A. Vasil’ev E. V. Dzyubinskaya D. B. Kiselevsky A. V. Nesov 《Biochemistry. Biokhimii?a》2010,75(2):257-263
Addition of chitosan or H2O2 caused destruction of nuclei of epidermal cells (EC) in the epidermis isolated from pea leaves. Phenol, a substrate of the
apoplastic peroxidase-oxidase, in concentrations of 10−10–10−6 M prevented the destructive effect of chitosan. Phenolic compounds 2,4-dichlorophenol, catechol, and salicylic acid, phenolic
uncouplers of oxidative phosphorylation pentachlorophenol and 2,4-dinitrophenol, and a non-phenolic uncoupler carbonyl cyanide
m-chlorophenylhydrazone, but not tyrosine or guaiacol, displayed similar protective effects. A further increase in concentrations
of the phenolic compounds abolished their protective effects against chitosan. Malate, a substrate of the apoplastic malate
dehydrogenase, replenished the pool of apoplastic NADH that is a substrate of peroxidase-oxidase, prevented the chitosan-induced
destruction of the EC nuclei, and removed the deleterious effect of the increased concentration of phenol (0.1 mM). Methylene
Blue, benzoquinone, and N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD) capable of supporting the optimal catalytic action of peroxidase-oxidase cancelled the destructive
effect of chitosan on the EC nuclei. The NADH-oxidizing combination of TMPD with ferricyanide promoted the chitosan-induced
destruction of the nuclei. The data suggest that the apoplastic peroxidase-oxidase is involved in the antioxidant protection
of EC against chitosan and H2O2. 相似文献
3.
Selective effects of H<Subscript>2</Subscript>O<Subscript>2</Subscript> on cyanobacterial photosynthesis 总被引:3,自引:0,他引:3
The sensitivity of phytoplankton species for hydrogen peroxide (H2O2) was analyzed by pulse amplitude modulated (PAM) fluorometry. The inhibition of photosynthesis was more severe in five tested
cyanobacterial species than in three green algal species and one diatom species. Hence the inhibitory effect of H2O2 is especially pronounced for cyanobacteria. A specific damage of the photosynthetic apparatus was demonstrated by changes
in 77 K fluorescence emission spectra. Different handling of oxidative stress and different cell structure are responsible
for the different susceptibility to H2O2 between cyanobacteria and other phytoplankton species. This principle may be potentially employed in the development of new
agents to combat cyanobacterial bloom formation in water reservoirs. 相似文献
4.
Jan Kubi 《Acta Physiologiae Plantarum》2005,27(3):289-295
Barley (Hordeum vulgare) seedlings were treated with spermidine prior to water deficit to determine whether this polyamine is able to affect the
activity of superoxide dismutase -SOD (EC 1.15.1.1) responsible for hydrogen peroxide and superoxide radical level.
Short-term dehydration (24h) resulted in decrease of the SOD specific activity and a distinct increase in the superoxide anion
and hydrogen peroxide contents. Polyamine treatment caused a substantial reduction in the contents of these two stress-raised
reactive oxygen species and thereby lowered the oxidative stress in plant cells. Antioxidant system as an important component
of the water-stress-protective mechanism can be changed by polyamines, able to moderate the radical scavenging system and
to lessen in this way the oxidative stress. 相似文献
5.
Generation of reactive oxygen species (ROS) and activities of antioxidant enzymes (catalase, peroxidase, ascorbate peroxidase)
in pea (Pisum sativum L.) and soybean (Glycine max L.) under hypoxia (3–24 h) and high CO2 concentration in medium were studied. In sensitive to hypoxia pea seedlings, hypoxia enhanced markedly production of superoxide
anion-radical, hydroperoxides, and especially hydrogen peroxide. In more tolerant soybean plants, these changes were less
pronounced. During first hours of hypoxia, activity of lipoxygenase in plant cells increased. This allows a suggestion that
this enzyme is involved in the processes of hydroperoxide accumulation in plant tissues under oxygen deficit. In pea and soybean
plants, a correlation between tolerance to hypoxia, the rate of ROS generation, and antioxidant enzyme activities was established.
During the first hours of hypoxia, the catalase activity in soybean plants increased stronger than in sensitive to hypoxia
pea plants. At longer exposure to hypoxia (24 h), peroxidases started to play the higher role in cell defense against hypoxia,
but only in soybean plants. The medium with the higher CO2 content induced higher changes in the processes of ROS accumulation and activities of lipoxygenase and antioxidant enzymes.
This permits us to refer CO2, accumulated as a product of respiration in the cells, to low-molecular signal molecules switching on plant adaptation to
hypoxic stress. 相似文献
6.
Kluge M Ullrich R Dolge C Scheibner K Hofrichter M 《Applied microbiology and biotechnology》2009,81(6):1071-1076
Agrocybe aegerita peroxidase/peroxygenase (AaP) is an extracellular fungal biocatalyst that selectively hydroxylates the aromatic ring of naphthalene.
Under alkaline conditions, the reaction proceeds via the formation of an intermediary product with a molecular mass of 144
and a characteristic UV absorption spectrum (A
max 210, 267, and 303 nm). The compound was semistable at pH 9 but spontaneously hydrolyzed under acidic conditions (pH <7) into
1-naphthol as major product and traces of 2-naphthol. Based on these findings and literature data, we propose naphthalene
1,2-oxide as the primary product of AaP-catalyzed oxygenation of naphthalene. Using 18O-labeled hydrogen peroxide, the origin of the oxygen atom transferred to naphthalene was proved to be the peroxide that acts
both as oxidant (primary electron acceptor) and oxygen source. 相似文献
7.
G. R. Rodríguez L. Sequin G. R. Pratta R. Zorzoli L. A. Picardi 《Biologia Plantarum》2008,52(3):548-552
Pericarp polypeptide profiles were analyzed at three ripening stages in the F1 hybrid and the F2 population from the cross between the accessions: LA1385 (Lycopersicon esculentum var. cerasiforme) and 804627 (L. esculentum, a homozygous genotype for the nor mutant). Six polymorphic polypeptides were observed in LA1385, while no polymorphic polypeptides among ripening stages was
observed in 804627. On the other hand, some polypeptides in the F1 hybrid were not observed in the parents whereas others were present in both parental genotypes and were unnoticeable in the
hybrid genotype. From a cluster analysis on the protein profiles of the F2 population, the differential expression of proteins allowed to distinguish mature green (MG) stage from the others two stages,
while for breaker stage (BR) and red ripe stage, the genetic background was more important in forming groups. The differential
expression of proteins could be associated with fruit morphology traits such as a 72 kDa polypeptide present in MG stage with
fruit diameter, height and mass and a 47 kDa polypeptide found in BR with fruit shelf life. 相似文献
8.
Reactive oxygen species (ROS), namely superoxide radical (O2
−) and hydrogen peroxide (H2O2) are generated when plant tissues endure a variety of environmental stresses, including light stress. The extremely short
life times of ROS makes the study of their production in planta very difficult. The use of ROS-specific tracer dyes, 3-3′
diaminobenzidine and nitroblue tetrazolium, together with high-resolution imaging provides the opportunity to identify sites
of photooxidative stress response by ROS accumulation. This technique was applied to grapevine during the first 7 days after
transfer from in vitro to ex vitro under an irradiance 4-fold higher than in vitro. ROS accumulation was detected in the first
days of analysis, which gradually decreased to levels comparable to greenhouse leaves. O2
− was uniformly distributed while H2O2 accumulated preferentially in veins, wounds and stomatal guard and surrounding cells. To evaluate the role of H2O2 in stomatal functioning and its crosstalk with abscisic acid (ABA) we focused on the percentage of coloured structures, stomatal
aperture and ABA concentration. We propose that the high H2O2 level triggered by increased light is responsible for the activation of a signalling pathway over stomatal cells, in a process
apparently irrespective of ABA regulation prior to root protrusion. This could explain the gain of function of a low yet consistent
percentage of stomatal cells, essential for plant survival during the ontogenic period in analysis. 相似文献
9.
Xiao-hui Chen Xue Zhou Xiao-yu Yang Zhi-bin Zhou Di-han Lu Ying Tang Ze-min Ling Li-hua Zhou Xia Feng 《Cellular and molecular neurobiology》2016,36(4):541-551
Propofol (2,6-diisopropylphenol) is a widely used general anesthetic with anti-oxidant activities. This study aims to investigate protective capacity of propofol against hydrogen peroxide (H2O2)-induced oxidative injury in neural cells and whether the anti-oxidative effects of propofol occur through a mechanism involving the modulation of NADPH oxidase (NOX) in a manner of calcium-dependent. The rat differentiated PC12 cell was subjected to H2O2 exposure for 24 h to mimic a neuronal in vitro model of oxidative injury. Our data demonstrated that pretreatment of PC12 cells with propofol significantly reversed the H2O2-induced decrease in cell viability, prevented H2O2-induced morphological changes, and reduced the ratio of apoptotic cells. We further found that propofol attenuated the accumulation of malondialdehyde (biomarker of oxidative stress), counteracted the overexpression of NOX core subunit gp91phox (NOX2) as well as the NOX activity following H2O2 exposure in PC12 cells. In addition, blocking of L-type Ca2+ channels with nimodipine reduced H2O2-induced overexpression of NOX2 and caspase-3 activation in PC12 cells. Moreover, NOX inhibitor apocynin alone or plus propofol neither induces a significant downregulation of NOX activity nor increases cell viability compared with propofol alone in the PC12 cells exposed to H2O2. These results demonstrate that the protective effects of propofol against oxidative injury in PC12 cells are mediated, at least in part, through inhibition of Ca2+-dependent NADPH oxidase. 相似文献
10.
H<Subscript>2</Subscript>O<Subscript>2</Subscript>-induced higher order chromatin degradation: A novel mechanism of oxidative genotoxicity 总被引:2,自引:0,他引:2
Konat GW 《Journal of biosciences》2003,28(1):57-60
The genotoxicity of reactive oxygen species (ROS) is well established. The underlying mechanism involves oxidation of DNA by ROS. However, we have recently shown that hydrogen peroxide (H2O2), the major mediator of oxidative stress, can also cause genomic damage indirectly. Thus, H2O2 at pathologically relevant concentrations rapidly induces higher order chromatin degradation (HOCD), i.e. enzymatic excision of chromatin loops and their oligomers at matrix-attachment regions. The activation of endonuclease that catalyzes HOCD is a signalling event triggered specifically by H2O2. The activation is not mediated by an influx of calcium ions, but resting concentrations of intracellular calcium ions are required for the maintenance of the endonuclease in an active form. Although H2O2-induced HOCD can efficiently dismantle the genome leading to cell death, under sublethal oxidative stress conditions H2O2-induced HOCD may be the major source of somatic mutations. 相似文献
11.
Marcos Roberto de Oliveira Gustavo da Costa Ferreira Alessandra Peres Simone Morelo Dal Bosco 《Molecular neurobiology》2018,55(2):968-979
The phenolic diterpene carnosic acid (CA, C20H28O4) exerts antioxidant, anti-inflammatory, anti-apoptotic, and anti-cancer effects in mammalian cells. CA activates the nuclear factor erythroid 2-related factor 2 (Nrf2), among other signaling pathways, and restores cell viability in several in vitro and in vivo experimental models. We have previously reported that CA affords mitochondrial protection against various chemical challenges. However, it was not clear yet whether CA would prevent chemically induced impairment of the tricarboxylic acid cycle (TCA) function in mammalian cells. In the present work, we found that a pretreatment of human neuroblastoma SH-SY5Y cells with CA at 1 μM for 12 h prevented the hydrogen peroxide (H2O2)-induced impairment of the TCA enzymes (aconitase, α-ketoglutarate dehydrogenase (α-KGDH), succinate dehydrogenase (SDH)) and abolished the inhibition of the complexes I and V and restored the levels of ATP by a mechanism associated with Nrf2. CA also exhibited antioxidant abilities by enhancing the levels of reduced glutathione (GSH) and decreasing the content oxidative stress markers (cellular 8-oxo-2′-deoxyguanosine (8-oxo-dG), and mitochondrial malondialdehyde (MDA), protein carbonyl, and 3-nitrotyrosine). Silencing of Nrf2 by small interfering RNA (siRNA) abrogated the protective effects elicited by CA in mitochondria of SH-SY5Y cells. Therefore, CA prevented the H2O2-triggered mitochondrial impairment by an Nrf2-dependent mechanism. The specific role of Nrf2 in ameliorating the function of TCA enzymes function needs further research. 相似文献
12.
Qien Li Zhaofeng Wang Yanning Zhao Xiaochen Zhang Shuaijun Zhang Letao Bo Yao Wang Yingfeng Ding Lizhe An 《Plant cell reports》2016,35(5):1155-1168
Key message
In hulless barley, H 2 S mediated increases in H 2 O 2 induced by putrescine, and their interaction enhanced tolerance to UV-B by maintaining redox homeostasis and promoting the accumulation of UV-absorbing compounds.Abstract
This study investigated the possible relationship between putrescence (Put), hydrogen sulfide (H2S) and hydrogen peroxide (H2O2) as well as the underlying mechanism of their interaction in reducing UV-B induced damage. UV-B radiation increased electrolyte leakage (EL) and the levels of malondialdehyde (MDA) and UV-absorbing compounds but reduced antioxidant enzyme activities and glutathione (GSH) and ascorbic acid (AsA) contents. Exogenous application of Put, H2S or H2O2 reduced some of the above-mentioned negative effects, but were enhanced by the addition of Put, H2S and H2O2 inhibitors. Moreover, the protective effect of Put against UV-B radiation-induced damage to hulless barley was diminished by dl-propargylglycine (PAG, a H2S biosynthesis inhibitor), hydroxylamine (HT, a H2S scavenger), diphenylene iodonium (DPI, a PM-NADPH oxidase inhibitor) and dimethylthiourea (DMTU, a ROS scavenger), and the effect of Put on H2O2 accumulation was abolished by HT. Taken together, as the downstream component of the Put signaling pathway, H2S mediated H2O2 accumulation, and H2O2 induced the accumulation of UV-absorbing compounds and maintained redox homeostasis under UV-B stress, thereby increasing the tolerance of hulless barley seedlings to UV-B stress.13.
The effect of exogenous hydrogen peroxide (H2O2) on mitotic activity and chromosomal aberrations in root tip meristems of barley (Hordeum vulgare L. var. Tokak 157/37) germinated under salinity was analyzed. The inhibitory effect of salinity on mitotic index and the
frequency of chromosomal aberrations increased with increasing salt concentration (0.00 control, 0.35, 0.40, 0.45 M, molal
NaCl). The frequency of chromosomal aberrations of seeds germinated in medium with 0.40 M NaCl after pretreatment with H2O2 (30 μM, micromolal) was significantly higher than the control group. The highest concentration of NaCl (0.45 M) together
with H2O2 caused total inhibition of germination. In this study, the intention was to determine the performance of H2O2 in alleviating detrimental effect of salt stress on mitotic activity and chromosomal aberrations. However, H2O2 did not reduce the detrimental effect of NaCl on these parameters. Also, it caused higher chromotoxic effect compared to
those of control groups. 相似文献
14.
E. O. Fedina F. G. Karimova I. A. Tarchevsky I. Yu. Toropygin V. A. Khripach 《Russian Journal of Plant Physiology》2008,55(2):193-200
Two-dimensional electrophoresis was used to separate proteins from crude extracts of pea (Pisum sativum L.) leaves, and thus isolated proteins were subjected to Western blot analysis with monoclonal antibodies against PY20 phosphotyrosine polypeptides. This analysis revealed 44 polypeptides phosphorylated on tyrosine residues. Phosphorylation of some of these proteins was changed under the action of epibrassinolide. Some of these polypeptides were identified by means of MALDI-TOF MS analysis. The results indicate that eight of these proteins belong to the Calvin cycle enzymes, namely, the isoforms of Rubisco large and small subunits, fructose-1,6-phosphate aldolases 1 and 2, and the precursor of α-subunit of Rubisco-binding protein. The observed changes in phosphorylation of these proteins may partly explain the effects of brassinosteroids on photosynthesis. The tyrosine phosphorylation sites were identified in silico for the fragments of polypeptides examined. 相似文献
15.
Ai Zhen Zhilong Bie Yuan Huang Zhixiong Liu Bo Lei 《Acta Physiologiae Plantarum》2011,33(6):2311-2319
Plant growth, photosynthetic parameters, chloroplast ultrastructure, and the ascorbate-glutathione cycle system in chloroplasts
of self-grafted and rootstock-grafted cucumber leaves were investigated. Grafted plants were grown hydroponically and were
exposed to 0, 50, and 100 mM NaCl concentrations for 10 days. Under NaCl stress, the hydrogen peroxide (H2O2) content in cucumber chloroplasts increased, the chloroplast ultrastructure was damaged, and the gas stomatal conductance,
intercellular CO2 concentration, as well as shoot dry weight, plant height, stem diameter, leaf area, and leaf relative water content were
inhibited, whereas these changes were less severe in rootstock-grafted plants. The activities of ascorbate peroxidase (APX;
EC 1.11.1.11), glutathione reductase (GR; EC 1.6.4.2), and dehydroascorbate reductase (DHAR EC 1.8.5.1) were higher in the
chloroplasts of rootstock-grafted plants compared with those of self-grafted plants under 50 and 100 mM NaCl. Similar trends
were shown in leaf net CO2 assimilation rate and transpiration rate, as well as reduced glutathione content under 100 mM NaCl. Results suggest that
rootstock grafting enhances the H2O2-scavenging capacity of the ascorbate–glutathione cycle in cucumber chloroplasts under NaCl stress, thereby protecting the
chloroplast structure and improving the photosynthetic performance of cucumber leaves. As a result, cucumber growth is promoted. 相似文献
16.
L. A. Tavadyan K. A. Galoian L. A. Harutunyan H. G. Tonikyan A. A. Galoyan 《Neurochemical research》2010,35(6):947-952
Chemical mechanisms of antioxidant and electron donating function of the hypothalamic proline-rich polypeptides have been
clarified on the molecular level. The antioxidant-chelating property of Galarmin and Gx-NH2 was established by their capability to inhibit copper(II) dichloride catalyzed H2O2 decomposition, thus preventing formation of HO• and HOO• radicals. The antiradical activity of Galarmin and Gx-NH2 was determined by their ability to react with 2,2-diphenyl-1-picrylhydrazyl radical applying differential pulse voltammetry
and UV–Vis spectrophotometry methods. Galarmin manifest antiradical activity towards 2,2-diphenyl-1-picrylhydrazyl radical,
depending on the existence of phenolic OH group in tyrosine residue at the end of the molecule. The presence of antiradical
activity and reduction properties of Galarmin are confirmed by the existence of an oxidation specific peak in voltammograms
made by differential pulse voltammetry at E
∘ = 0.795 V vs. Ag/Ag+ aq. 相似文献
17.
18.
Tungsten belong to heavy metal group, which physiological, biochemical, and molecular action mechanisms are essentially unstudied despite metal wide application in light, heavy, and military industries and the gradual accumulation in the environment. Protein phosphorylation/dephosphorylation (one of the most important posttranslational modifications) is a highly conserved mechanism of intracellular signaling and regulation of many processes of cell activity. Protein tyrosine phosphorylation/dephosphorylation is required for the cell cycle processing, plant growth and differentiation. In this work, the effects of sodium tungstate on pea (Pisum sativum L. cv. Truzhenik) root growth, protein tyrosine phosphorylation, and phosphatase activity in the roots were studied. It was shown that sodium tungstate suppressed growth, changed the mitotic index in the root meristem, and delayed cells at some mitosis phases. Under the influence of tungstate, hydrogen peroxide accumulated in the roots and phosphatase activity was inhibited. It was established by two-dimension electrophoresis and immunoblotting with the highly specific to phosphotyrosine antibody (PY20) that tungstate treatment increased both the number of such proteins and their specific phosphorylation. It is supposed that the inhibition of protein tyrosine phosphatases was one of the reasons for tungstateinduced pea root growth inhibition. 相似文献
19.
To improve xanthan gum productivity, a strategy of adding hydrogen peroxide (H2O2) was studied. The method could intensify oxygen supply through degradation of H2O2 to oxygen (O2). In shake flask testing, the xanthan gum yield reached 2.8% (improved by 39.4%) when adding 12.5 mM H2O2 after 24 h of fermentation. In fermentor testing, it was obvious that the oxygen conditions varied with the H2O2 addition time. Eventually, gum yield of 4.2% (w/w) was achieved (increased by 27.3%). Compared with the method of intense
mixing and increasing the air flow rate, adding H2O2 to improve the dissolved oxygen concentration was more effective and much better. Moreover, addition of H2O2 improved the quality of xanthan gum; the pyruvate content of xanthan was 4.4% (w/w), higher than that of the control (3.2%). 相似文献
20.
The effect of foliar pretreatment by hydrogen peroxide (H2O2) at low concentrations of 0, 5, 10, and 15 mM on the chilling tolerance of two Zoysia cultivars, manilagrass (Zoysia matrella) and mascarenegrass (Zoysia tenuifolia), was studied. The optimal concentration for H2O2 pretreatment was 10 mM, as demonstrated by the lowest malondialdehyde (MDA) content and electrolyte leakage (EL) levels and
higher protein content under chilling stress (7°C/2°C, day/night). Prior to initiation of chilling, exogenous 10 mM H2O2 significantly increased catalase (CAT), ascorbate peroxidase (APX), glutathione-dependent peroxidases (GPX), and glutathione-S-transferase (GST) activities in manilagrass, and guaiacol peroxidase (POD), APX, and glutathione reductase (GR) activities
in mascarenegrass, suggesting that H2O2 may act as a signaling molecule, inducing protective metabolic responses against further oxidative damage due to chilling.
Under further stress, optimal pretreatments alleviated the increase of H2O2 level and the decrease of turfgrass quality, and improved CAT, POD, APX, GR, and GPX activities, with especially significant
enhancement of APX and GPX activities from the initiation to end of chilling. These antioxidative enzymes were likely the
important factors for acquisition of tolerance to chilling stress in the two Zoysia cultivars. Our results showed that pretreatment with H2O2 at appropriate concentration may improve the tolerance of warm-season Zoysia grasses to chilling stress, and that manilagrass had better tolerance to chilling, as evaluated by lower MDA and EL, and
better turfgrass quality, regardless of the pretreatment applied. 相似文献