长江口盐沼海三棱藨草在高程梯度上的生物量分配(英文)

海三棱草 (ScirpusmariqueterTangetZhang)是一种典型的先锋植物。它能占据于长江口盐沼的光滩上 ,生境非常恶劣。为探索该物种在高度环境胁迫下的生活史策略 ,测定了它在高程梯度上的生物量分配 ,以及有关形态学参数。结果发现海三棱草在中位高程时密度和单株生物量最高 ,生长最好。在由低及高的高程梯度上 ,球茎、根状茎的生物量分配比例逐渐下降 ,表明植物体在光滩前沿采取保守策略 ;而花序的比例则逐渐上升 ,表明植物在生活史过程中 ,由无性生殖向有性生殖的转变。这种转变可能有利于种群的扩散和生存。另外 ,相关分析表明 ,有性生殖性状与无性生殖、生长性状呈负相关 ,但无性生殖与生长性状间的关系难以确定 ,可能由于球茎在不同生活史阶段的不同功能引起。     

Different Responses of Sexual and Asexual Reproduction of Arundinella hirta to Flooding

The sexual and asexual reproductive features of the graminoid species Arundinella hirta growing at riversides of the Jialing River were analyzed.It was found that the total seed mass,seed number per plant,and sexual reproductive allocation of A.hirta decreased with decreasing bank elevation,and plants growing at the lowest elevations of banks subjected to intense flooding did not show sexual reproduction.The total plant biomass and the number of ramifications per plant increased with flooding intensity,which implies that,contrary to sexual reproduction,asexual reproduction of A.hirta was enhanced by flooding.     

Variations in sexual and asexual reproduction of Scirpus mariqueter along an elevational gradient

In order to assess the importance of sexual and asexual reproduction during the life history of Scirpus mariqueter, its reproductive and growth characters were concurrently examined along an elevational gradient (from low elevation to high elevation). The proportions of flowering shoot and inflorescence mass, seed : flower ratio and seed weight were used to quantify the investment in sexual reproduction. The proportions of current-year shoot and rhizome mass were used to quantify the investment in asexual reproduction, and the proportion of corm mass was used for growth, respectively. It was found that vegetative propagation predominated at low elevation, whereas sexual reproduction predominated at high elevation; and that sexual reproduction increased with declining asexual reproduction along the gradient. The results suggest that asexual reproduction is relatively favored in the early life stage, whereas sexual reproduction is favored when the population becomes mature and aged, probably because of the functional differentiation between the two reproductive types. Sexual productive characters (i.e. the proportions of flowering shoot and inflorescence mass) were negatively correlated to both growth and asexual reproductive characters along the gradient, indicating there might exist some trade-offs among growth, sexual and asexual reproduction during the life history. However, no obvious pattern was found between asexual reproductive characters and growth characters along the elevational gradient, possibly because of the varied relationships between them at different life stages. The variations in sexual and asexual reproduction in the species and the relationship between them are thought to be of great significance for local population growth, species persistence and evolution.     

Different Responses of Sexual and Asexual Reproduction of Arundinella hirta to Flooding

The sexual and asexual reproductive features of the graminoid species Arundinella hirta growing at riversides of the Jialing River were analyzed. It was found that the total seed mass, seed number per plant, and sexual reproductive allocation of A. hirta decreased with decreasing bank elevation, and plants growing at the lowest elevations of banks subjected to intense flooding did not show sexual reproduction. The total plant biomass and the number of ramifications per plant increased with flooding intensity, which implies that, contrary to sexual reproduction, asexual reproduction of A. hirtawas enhanced by flooding. Translated from the Journal of Southwest China Normal University (Natural Science), 2005, 30(2) (in Chinese)     

Genetic signature of amphimixis allows for the detection and fine scale localization of sexual reproduction events in a mainly parthenogenetic nematode

Asexuality is an important mode of reproduction in eukaryotic taxa and has a theoretical advantage over sexual reproduction because of the increased ability to propagate genes. Despite this advantage, hidden signs of cryptic sex have been discovered in the genomes of asexual organisms. This has provided an interesting way to address the evolutionary impact of sex in plant and animal populations. However, the identification of rare sexual reproduction events in mainly asexual species has remained a challenging task. We examined the reproductive history in populations of the plant parasitic nematode Xiphinema index by genotyping individuals collected from six grapevine fields using seven microsatellite markers. A high level of linkage disequilibrium and heterozygous excess suggested a clonality rate of 95–100%. However, we also detected rare sexual reproduction events within these highly clonal populations. By combining highly polymorphic markers with an appropriate hierarchical sampling, and using both Bayesian and multivariate analysis with phylogenetic reconstructions, we were able to identify a small number of sexually produced individuals at the overlapping zones between different genetic clusters. This suggested that sexual reproduction was favoured when and where two nematode patches came into contact. Among fields, a high degree of genetic differentiation indicated a low level of gene flow between populations. Rare genotypes that were shared by several populations suggested passive dispersal by human activities, possibly through the introduction of infected plants from nurseries. We conclude that our method can be used to detect and locate sexual events in various predominantly asexual species.     

不同生境下珠芽蓼（蓼科）的繁殖策略比较

在青藏高原横断山区以海拔相同的高山流石滩和高寒草甸中的珠芽蓼（Polygonum viviparum）居群为研究对象,探讨该物种在不同生境条件下的繁殖策略以及无性繁殖和有性繁殖间的关系。结果发现,流石滩居群的珠芽蓼植株高度和珠芽数量显著低于高寒草甸居群,而珠芽和花总数量、花数量和每个花序上的花比例却显著高于高寒草甸居群。研究结果表明珠芽蓼在环境更为恶劣的高山流石滩生境中增加了对繁殖器官和花资源的投资,说明在高山植物中繁殖优先于营养生长,且有性繁殖比无性繁殖具有更为重要的作用。而对每个植株上花数量和珠芽数量的统计结果表明,两者呈显著的负相关关系,进一步证明了同一植株内无性繁殖和有性繁殖的权衡关系。     

Restriction of sexual reproduction in the moss Racomitrium lanuginosum along an elevational gradient

1. Terrestrial plant populations located at the margins of species’ distributions often display reduced sexual reproduction and an increased reliance on asexual reproduction. One hypothesis to explain this phenomenon is that the decline is associated with environmental effects on the energetic costs to produce reproductive organs.
2. In order to clarify the changing processes of sexual reproduction along an elevational gradient, we investigated the sexual reproductive parameters, such as the number of sporophytes and gametangia, in Racomitrium lanuginosum, a dioicous moss found on Mt. Fuji.
3. Matured sporophytes were present only below 3,000 m, and the number of sporophytes per shoot tended to be lower at higher elevation habitats. The numbers of male inflorescences per shoot and antheridia per inflorescence and shoot significantly decreased with increasing elevation. In contrast, the numbers of female inflorescences per shoot and archegonia per inflorescence and shoot varied little across elevations.
4. Synthesis. Our results suggest that the reasons for this limitation are assumed to be limitations in sporophyte development that result in abortion, and the spatial segregation between males and females. Possible reasons for the abortion of sporophytes are the inhibitory effects of low air temperature, a shortened growth period, and winter environmental conditions at higher elevations. Remarkable differences between male and female on various reproductive parameters found in this study are thought to affect the mode of sexual reproduction under the harsh environment. These differences between males and females may be caused by differences in the costs of production and development of gametangia, sensitivity to environmental stressors, and phenological patterns.

     

Modeling the impact of reproductive mode on masting

Masting is defined as the intermittent highly variable production of seed in a plant population. According to reproductive modes, that is, sexual and asexual reproduction, masting species can be separated into three groups, that is, (1) species, for example, bamboo, flower only once before they die; (2) species, for example, Fagus, reproduce sexually; and (3) species, for example, Stipa tenacissima, reproduce both sexually and asexually. Several theories have been proposed to explore the underlying mechanisms of masting. However, to our knowledge, no theory has been found to explain the mechanism of masting species that reproduce both sexually and asexually. Here we refine the Resource Budget Model by considering a trade‐off between sexual and asexual reproduction. Besides the depletion efficient (i.e., the ratio of the cost of seed setting and the cost of flowering), other factors, such as the annual remaining resource (i.e., the rest of the resource from the photosynthetic activity after allocating to growth and maintenance), the trade‐off between sexual and asexual reproduction, and the reproductive thresholds, also affect masting. Moreover, two potential reproductive strategies are found to explain the mechanisms: (1) When the annual remaining resource is relatively low, plants reproduce asexually and a part of the resource is accumulated as the cost of asexual reproduction is less than the annual remaining resource. Plants flower and set fruits once the accumulated resource exceeds the threshold of sexual reproduction; (2) when the annual remaining resource is relatively high, and the accumulated resource surpasses the threshold of sexual reproduction, masting occurs. Remarkably, under certain depletion efficient, more investigation in sexual reproduction will lead plants to reproduce periodically. Additionally, plants investigate less resource to reproduce periodically when depletion efficient keeps increasing as plants can reproduce efficiently. Overall, our study provides new insights into the interpretation of masting, especially for species that reproduce both sexually and asexually.     

太行花生殖生物学研究Ⅱ有性生殖与无性生殖的调查与研究

通过原产地调查,引种试验,扦插及种子萌发等实验,研究了太行花的繁殖及扩散方式。结果表明,太行花有两条生殖途径：一条是有性生殖;另一条是无性生殖。在原产地因生境条件特殊,有性生殖能力弱,主要靠无性生殖扩大种群。在北京人工栽培条件下,两种生殖方式都能正常进行。     

Reproductive strategy, clonal structure and genetic diversity in populations of the aquatic macrophyte Sparganium emersum in river systems

Many aquatic and riparian plant species are characterized by the ability to reproduce both sexually and asexually. Yet, little is known about how spatial variation in sexual and asexual reproduction affects the genotypic diversity within populations of aquatic and riparian plants. We used six polymorphic microsatellites to examine the genetic diversity within and differentiation among 17 populations (606 individuals) of Sparganium emersum, in two Dutch-German rivers. Our study revealed a striking difference between rivers in the mode of reproduction (sexual vs. asexual) within S. emersum populations. The mode of reproduction was strongly related to locally reigning hydrodynamic conditions. Sexually reproducing populations exhibited a greater number of multilocus genotypes compared to asexual populations. The regional population structure suggested higher levels of gene flow among sexually reproducing populations compared to clonal populations. Gene flow was mainly mediated via hydrochoric dispersal of generative propagules (seeds), impeding genetic differentiation among populations even over river distances up to 50 km. Although evidence for hydrochoric dispersal of vegetative propagules (clonal plant fragments) was found, this mechanism appeared to be relatively less important. Bayesian-based assignment procedures revealed a number of immigrants, originating from outside our study area, suggesting intercatchment plant dispersal, possibly the result of waterfowl-mediated seed dispersal. This study demonstrates how variation in local environmental conditions in river systems, resulting in shifting balances of sexual vs. asexual reproduction within populations, will affect the genotypic diversity within populations. This study furthermore cautions against generalizations about dispersal of riparian plant species in river systems.     

The Tangled Bank: The maintenance of sexual reproduction through competitive interactions

The maintenance of sexual reproduction is discussed using a model based on the familiar Lotka-Volterra competition equations. Both the equilibrium and the stability conditions that allow a sexual population to resist invasion by a single asexual clone are considered. The equilibrium conditions give results similar to previous models: When the cost of sex, within phenotype niche width, and environmental variance are low, the sexual population coexists with the asexual clone and remains at a high density. However, the asexual clone is never completely excluded. Analysis of the stability conditions shows a different picture: The introduction of an asexual clone considerably reduces the stability of the community. However, owing to its larger total niche width, the sexual population exists partly in a “competitor-free space” where the asexual clone has almost no influence on the outcome of the interactions. Therefore the asexual clone is less stable than the sexual population and has a higher probability of extinction. In contrast, the sexual population does not become extinct, since the extreme phenotypes remain at a stable, though low, density, and the central phenotypes, where stability is low, are recreated every generation through recombination. I therefore conclude that the ecological conditions under which sexual reproduction is favored over asexual reproduction are fairly easily attained and are more general than previous analyses had suggested.     

Trade-offs between sexual and clonal reproduction in an aquatic plant: experimental manipulations vs. phenotypic correlations

That trade-offs result from the allocation of limited resources is a central concept of life history evolution. We quantified trade-offs between sexual and clonal reproduction in the aquatic plant, Butomus umbellatus, by experimentally manipulating sexual investment in two distinct nutrient environments. Increasing seed production caused a significant but nonlinear trade-off. Pollinating half of all flowers strongly reduced clonal bulbil production, but pollinating the remaining flowers did not cause any further trade-off. Trade-offs were not stronger under low nutrient conditions that clearly limited plant growth. Experimentally induced trade-offs were not reflected in negative phenotypic correlations between sexual and clonal allocation among plants within eight populations grown in a uniform greenhouse environment. Diminishing effects of increased sexual allocation plus a lack of accord between experimental manipulations and phenotypic correlations suggest that trade-offs between sexual and clonal reproduction are unlikely to constrain the evolution of reproductive strategy in this species.     

Multiple-scale environmental modulation of lichen reproduction

It is necessary to understand how environmental changes affect plant fitness to predict survival of a species, but this knowledge is scarce for lichens and complicated by their formation of sexual and asexual reproductive structures. Are the presence and number of reproductive structures in Lobaria pulmonaria, a threatened lichen, dependent on thallus size, and is their formation sequential? Does any size-dependence and sequential formation vary along a climate gradient? Generalized linear mixed models were used to explore the effect of environmental predictors on the size and presence/abundance of each reproductive structure and to determine the probability of a given-sized thallus to develop any reproductive structure. The largest individuals are more likely to develop reproductive structures, and the lichen uses a mixed strategy of early asexual reproduction and late sexual. Macro and microclimatic variables also influenced reproductive capacity. Relationships among climate conditions and lichen size and reproductive capacity can compromise the future viability of the species in the most southern populations of Europe.     

Sexual and asexual reproduction of the holothurian Stichopus chloronotus (Echinodermata): a comparison between La Réunion (Indian Ocean) and east Australia (Pacific Ocean)

Summary

Stichopus chloronotus (Brandt, 1835) is one among nine aspidochirotide holothurian species known to reproduce both sexually by broadcast spawning and asexually by transverse fission. New data on the sexual cycle of this species in La Réunion are presented here and information available on sexual and asexual reproduction in this species is summarised. Sexual reproduction on La Réunion shows a distinct seasonality with a main spawning period in the warm season (November-February). The spawning period the Great Barrier Reef appears to be at the same time. Some intriguing deviations from unity in sex-ratio, usually biased towards more male individuals, have been observed in both geographic regions (sex ratio at La Reunion 31:1). New data on the asexual reproduction of this species in La Réunion confirm the high rates of fission. The peak of asexual reproduction in both the Indian and Pacific Ocean was observed in winter (June-July). Thus, asexual reproduction in this species occurs outside the season for sexual reproduction. The rate of asexual reproduction appears to vary between sample locations. However, results of population genetic studies on S. chloronotus (Uthicke et al., 1999; Uthicke et al., 2001) indicated that in most populations investigated a maximum of about 60% of all individuals may be derived from sexual recruitment. Cluster analyses on genetic distances between populations grouped populations within Oceans together, with the exception of one sample from a nearshore reef of the GBR. Although genetic differences between the two regions exist, these are relatively small regarding the large geographic distance. We conclude that asexual reproduction in S. chloronotus is important to maintain local population sizes, but that larval exchange between populations mediated by sexual reproduction is important for colonisation of new areas and to provide connectivity between populations. Here, we present the first synthesis of these phenomena for a holothurian species.     

Effects of waterlogging and increased soil nutrients on growth and reproduction of Polygonum hydropiper in the hydro-fluctuation belt of the Three Gorges Reservoir Region

水淹和土壤养分是影响三峡库区消落带植物生长的主要环境因子。消落带不同高程的植物长期经历不同的淹水强度和土壤养分条件。该研究假设同一物种来自于消落带不同高程的植株可能产生性状分化, 从而对根部淹水和土壤养分变化具有不同的生长和繁殖响应策略。为了验证以上假设, 选取在三峡库区消落带高低高程均广泛分布的物种水蓼(Polygonum hydropiper)为研究对象, 采集自然种群的种子。在温室同质园条件下, 研究了根部水淹和土壤养分提升对高低高程水蓼植株生长和繁殖特性的影响。研究结果表明根部水淹显著或趋于显著降低了水蓼植株功能叶的叶长、叶宽、总分枝数、叶生物量、花生物量和总生物量; 低养分处理显著或趋于显著降低了水蓼植株的总节数、总分枝数、根生物量、花生物量和总生物量, 表明根部水淹和低土壤养分对水蓼的生长和繁殖能力具有抑制作用。同时, 根部水淹和土壤养分的交互作用显著影响植株的根生物量, 表明根部水淹条件下高土壤养分更有利于植株根生物量的积累。高高程植株的根生物量和叶生物量显著或趋于显著高于低高程植株, 而低高程植株的始花时间早于高高程植株, 且繁殖分配也显著高于高高程植株, 表明高低高程水蓼植株对资源的分配策略不同。该研究结果表明水蓼的生长和繁殖特性受根部水淹和土壤养分共同限制, 但对根部水淹条件下高土壤养分生境具有较好的适应性; 同时, 低高程植株可以通过调整其生长和繁殖特性以提高对所处生境胁迫的适应性。     

When sex is not enough: ecological correlates of resprouting capacity in congeneric tropical forest shrubs

In moist tropical forests resprouting may be an important component of life history, contributing to asexual reproduction through the clonal spread of individuals derived from shoot fragments. However, in contrast to other ecosystems where resprouting is common, the ecological correlates of resprouting capacity in tropical forests remain largely unexplored. In this study we characterized shade tolerance, resprouting capacity and sexual reproductive success of eight co-occurring Piper species from lowland forests of Panama. In field experiments we found that shade-tolerant Piper species had a higher capacity to regenerate from excised or pinned stem fragments than light-demanding species in both gap and understory light conditions. In contrast, shade-tolerant species had lower recruitment probabilities from seeds, as a consequence of lower initial seed viability, and lower seedling emergence rates. All Piper species needed gap conditions for successful seedling establishment. Of 8,000 seeds sown in the understory only 0.2% emerged. In gaps, seed germination of light-demanding species was between 10 and 50%, whereas for shade-tolerant species it was 0.5–9.8%. We propose that the capacity to reproduce asexually from resprouts could be adaptive for shade-tolerant species that are constantly exposed to damage from falling litter in the understory. Resprouting may allow Piper populations to persist and spread despite the high rate of pre-dispersal seed predation and low seed emergence rates. Across Piper species, we detected a trade-off between resprouting capacity and the annual viable seed production per plant but not with annual seed mass produced per plant. This suggests that species differences in sexual reproductive success may not necessarily result from differential resource allocation. Instead we suggest that low sexual reproductive success in the understory may in part reflect reduced genetic diversity in populations undergoing clonal growth, resulting in self-fertilization and in-breeding depression.     

根部水淹和土壤养分提升对三峡库区消落带水蓼生长和繁殖特性的影响

水淹和土壤养分是影响三峡库区消落带植物生长的主要环境因子。消落带不同高程的植物长期经历不同的淹水强度和土壤养分条件。该研究假设同一物种来自于消落带不同高程的植株可能产生性状分化, 从而对根部淹水和土壤养分变化具有不同的生长和繁殖响应策略。为了验证以上假设, 选取在三峡库区消落带高低高程均广泛分布的物种水蓼(Polygonum hydropiper)为研究对象, 采集自然种群的种子。在温室同质园条件下, 研究了根部水淹和土壤养分提升对高低高程水蓼植株生长和繁殖特性的影响。研究结果表明根部水淹显著或趋于显著降低了水蓼植株功能叶的叶长、叶宽、总分枝数、叶生物量、花生物量和总生物量; 低养分处理显著或趋于显著降低了水蓼植株的总节数、总分枝数、根生物量、花生物量和总生物量, 表明根部水淹和低土壤养分对水蓼的生长和繁殖能力具有抑制作用。同时, 根部水淹和土壤养分的交互作用显著影响植株的根生物量, 表明根部水淹条件下高土壤养分更有利于植株根生物量的积累。高高程植株的根生物量和叶生物量显著或趋于显著高于低高程植株, 而低高程植株的始花时间早于高高程植株, 且繁殖分配也显著高于高高程植株, 表明高低高程水蓼植株对资源的分配策略不同。该研究结果表明水蓼的生长和繁殖特性受根部水淹和土壤养分共同限制, 但对根部水淹条件下高土壤养分生境具有较好的适应性; 同时, 低高程植株可以通过调整其生长和繁殖特性以提高对所处生境胁迫的适应性。     

拟衣藻属一新种——中华拟衣藻及其生活史的研究

报道了团藻目衣藻科拟衣藻属一新种。此种从中国科学院武汉植物研究所一浇园粪缸中采得,在分离室内单种培养过程中,对其形态学和生活史进行了研究。营养细胞具有多个盘状色素体,与拟衣藻属已知的种类有明显的差别。经培养观察,作者发现除细胞分裂、无性生殖外,还有同宗异配式有性生殖,在生活史各阶段该藻中绿体中均未见蛋白核,因此,拟衣藻作为分类单元是一个有效的属。     

General environmental heterogeneity as the explanation of sexuality? Comparative study shows that ancient asexual taxa are associated with both biotically and abiotically homogeneous environments

Ecological theories of sexual reproduction assume that sexuality is advantageous in certain conditions, for example, in biotically or abiotically more heterogeneous environments. Such theories thus could be tested by comparative studies. However, the published results of these studies are rather unconvincing. Here, we present the results of a new comparative study based exclusively on the ancient asexual clades. The association with biotically or abiotically homogeneous environments in these asexual clades was compared with the same association in their sister, or closely related, sexual clades. Using the conservative definition of ancient asexuals (i.e., age >1 million years), we found eight pairs of taxa of sexual and asexual species, six differing in the heterogeneity of their inhabited environment on the basis of available data. The difference between the environmental type associated with the sexual and asexual species was then compared in an exact binomial test. The results showed that the majority of ancient asexual clades tend to be associated with biotically, abiotically, or both biotically and abiotically more homogeneous environments than their sexual controls. In the exploratory part of the study, we found that the ancient asexuals often have durable resting stages, enabling life in subjectively homogeneous environments, live in the absence of intense biotic interactions, and are very often sedentary, inhabiting benthos, and soil. The consequences of these findings for the ecological theories of sexual reproduction are discussed.     

Asexual and sexual reproductive strategies in clonal plants

Most plants can reproduce both sexually and asexually (or vegetatively),and the balance between the two reproductive modes may vary widely between and within species.Extensive clonal growth may affect the evolution of life history traits in many ways.First,in some clonal species,sexual reproduction and sex ratio vary largely among populations.Variation in sexual reproduction may strongly affect plant's adaptation to local environments and the evolution of the geographic range.Second,clonal growth can increase floral display,and thus pollinator attraction,while it may impose serious constraints and evolutionary challenges on plants through geitonogamy that may strongly influence pollen dispersal.Geitonogamous pollination can bring a cost to plant fitness through both female and male functions.Some co-evolutionary interactions,therefore,may exist between the spatial structure and the mating behavior of clonal plants.Finally,a trade-off may exist between sexual reproduction and clonal growth.Resource allocation to the two reproductive modes may depend on environmental conditions,competitive dominance,life span,and genetic factors.If different reproductive modes represent adaptive strategies for plants in different environments,we expect that most of the resources should be allocated to sexual reproduction in habitats with fluctuating environmental conditions and strong competition,while clonal growth should be dominant in stable habitats.Yet we know little about the consequence of natural selection on the two reproductive modes and factors which control the balance of the two reproductive modes.Future studies should investigate the reproductive strategies of clonal plants simultaneously from both sexual and asexual perspectives.