三分三的离体培养和植株再生

1植物名称三分三(AnisodusacutangulusC.Y.WuetC.Chen)。2材料类别无菌苗叶片。3培养条件(1)种子无菌萌发培养基:MS+6-BA0.5mg·L-1(单位下同);(2)愈伤组织和胚状体诱导培养基:MS+6-BA1.0+2,4-D0.5;(3)生根培养基:MS+NAA1.0。以上培养基均附加3%蔗糖和0.8%琼脂,pH5.8。培养温度为(25±1)℃,光照时间12h·d-1,光照强度为40μmol·m-2·s-1。4生长与分化情况5意义与进展三分三为茄科山莨菪属(也有列入安妮莎属)多年生草本植物,主要分布于我国云南西北部海拔2750～3000m的山区。根、茎、叶和种子均富含生物碱,味苦、辛,性温,有剧毒,具…     

刺芫荽的离体培养和植株再生

1植物名称刺芫荽(Eryngium foetidum L.),又名刺芹、野芫荽、缅芫荽、阿佤芫荽。2材料类别成熟种子。3培养条件基本培养基为MS培养基。(1)种子萌发培养基:MS;(2)芽的分化与增殖培养基:MS 6-BA 0.8 mg·L~(-1)(单位下同) NAA 0.03;(3)生根培养基:1/2MS。上述培养基中均加3%蔗糖、     

三尖杉的离体胚培养

1植物名称三尖杉(Cephalotaxus fortunei Hook.f.)。2材料类别离体胚。3培养条件芽诱导培养基:MS 6-BA2.0mg·L-1(单位下同) IAA1.0 NAA0.1;芽增殖培养基:MS 6-BA2.0 IAA1.0 NAA1.0;生根培养基:1/2MS IBA1.0 NAA0.5 KT0.2。以上各培养基     

梳唇石斛成熟胚的离体培养和植株再生

1植物名称梳唇石斛(Dendrobium strongylanthum Rchb.f.). 2材料类别成熟种子. 3培养条件以MS和1/2MS为基本培养基.(1)种子萌发培养基:MS NAA 0.2 mg·L-1(单位下同) 6-BA 0.4 马铃薯汁200g·L-1;(2)原球茎诱导增殖培养基:1/2MS 6-BA 0.5;(3)原球茎分化培养基:1/2MS 6-BA 0.5 NAA 0.5 椰子汁200g·L-1 ;(4)壮苗及生根培养基:1/2MS NAA 0.5 香蕉泥100g·L-1 .上述培养基均附加20 g·L-1 蔗糖、8 g·L-1琼脂,pH 5.5～5.8.培养温度为(24±2)℃,光照时间12 h·d-1,光照度1 000～2000 lx.     

日本牵牛茎段的离体培养及植株再生

1植物名称日本牵牛(Pharbitis nil). 2材料类别带叶柄幼嫩茎段. 3培养条件以MS为基本培养基.(1)丛生芽诱导培养基:MS KT 2.0 mg·L-1(单位下同) IBA 0.01;(2)增殖培养基:MS KT 1.0 IBA 0.01;(3)生根培养基:I/2MS NAA 0.5.以上培养基均添加30g·L-1蔗糖、6 g·L-1琼脂,pH 5.8.接种后先在培养箱中暗培养7 d,然后置于光下培养,培养温度为(25 1)℃,光照时间为12 h·d-1,光照度为2000 lx左右.     

山葵未成熟胚离体培养和植株再生

1　植物名称　山葵 (Ｅｕｔｒｅｍａｗａｓａｂｉ)日本品种岛根 3号。2　材料类别　开花 2 0ｄ后的荚果中剥出的未成熟种子 (种皮 未成熟胚 )。3　培养条件　基本培养基为自行设计的“贵山”(代号ＧＳ)配方[1 ] 。 ( 1 )诱导愈伤组织培养基为 :ＧＳ 6 ＢＡ 0 .1ｍｇ·Ｌ- 1 (单位下同 ) 2 ,4 Ｄ 1 ;( 2 )芽分化培养基为 :ＧＳ 6 ＢＡ 0 .5 ＺＴ 0 .5 ＩＢＡ0 .0 1 ;( 3)芽增殖培养基为 :ＧＳ 6 ＢＡ 0 .4;( 4 )生根培养基为 :ＧＳ ＩＢＡ 0 .1 ＮＡＡ 0 .1。以上培养基均含 3%蔗糖 [( 1 )含 2 % ]和 0 .6%琼脂粉 ,ｐＨ5 .8～ 5…     

蓝果树的离体培养和植株再生

1植物名称 蓝果树（Nyssa sinensis Oliv．）,又名紫树。 2材料类别 幼嫩枝条。 3培养条件 以MS为基本培养基。（1）腋芽诱导培养基：1／2MS（CaCl2全量）＋6-BA1．0mg·L^-1（单位下同）＋IBA0．1;（2）不定芽增殖培养基：MS＋6-BA1．0—2．0＋KT1．0＋IBA0．1-0.3;（3）壮苗培养基：MS＋6-BA0．5＋IBA0．2;     

山椒子离体植株再生

1植物名称山椒子（Uvaria grandiflora Roxb.）。 2材料类别 下胚轴、茎段。 3培养条件 （1）种子萌发培养基：1/2MS;（2）不定芽诱导培养基：MS＋BA2.0mg·L-1（单位下同）＋NAA0.1;（3）增殖培养基：MS＋BA1.0＋NAA0.1;     

峨眉含笑试管培养再生植株

1植物名称峨眉含笑(Michelia wilsonii Finet et Gagnep.)。2材料类别侧枝的顶芽。3培养条件诱导培养基:(1)MS 6-BA 1 mg·L~(-1) (单位下同) NAA 0.2;增殖培养基:(2)MS 6- BA 0.3 NAA 0.3;生根培养基:(3)1/2MS NAA 0.5 IBA 0.5。诱导培养基和增殖培养基加入3%蔗     

屋顶长生花的离体培养和植株再生

1植物名称屋顶长生花(Sempervivum tectorum). 2材料类别叶片. 3培养条件基本培养基为MS培养基.(1)诱导愈伤组织培养基:MS 2,4-D 2.0 mg·L-1(单位下同) 6-BA 2.0;(2)诱导分化培养基:MS 6-BA 2.0 NAA0.2 GA3 0.4;(3)继代增殖培养基:MS 6-BA0.3～4;(4)生根培养基:1/2MS NAA 0.2 IAA 1.0 0.3%活性炭.以上培养基均加入0.8%琼脂、4.5%蔗糖,pH 5.8.培养温度为(21±1)℃,光照度2500lx,光照时间14 h·d-1.     

Optimization of Callus Induction Conditions from Immature Embryos in Maize and Plant Regeneration

This research uses the immature embryos of inbred maize lines (GSH9901, Hi01, Hi02, and Chang 7-2) as receptor materials to establish the callus induction system. These inbred lines provide the receptor materials for the genetic regeneration of maize and the verification of the genetic functions of maize. The factor experiment and orthogonal experiments were used to investigate the impacts of different genotypes, immature embryo size, shield orientation, 2, 4-D concentration, proline concentration, and folic acid concentration on the induction rate of embryogenic callus tissue. A sensitivity experiment testing glyphosate (Bar) and an antibiotic (Cefotaxime sodium) were also conducted. The results indicate that the immature embryos of inbred maize line GSH9901 were the most effective for callus tissue induction, and the immature embryos with a length of 1.6-2.0 mm produce the best result. The upward shield face is more successful for the formation of induced callus. Using orthogonal analysis, we found that the optimal combination for the induction system was A₃ (2,4-D concentration 0.25 mg mL^-1 ), B₁C₃ (proline concentration 0.8 mg mL^-1 ), and D₂ (folate Concentration 0.5 mg mL^-1) and the induction rate reached 84%. We found that cold storage at 4 °C for 1 d is more conducive for the formation of embryogenic callus than the other treatments tested. The sensitivity experiment for callus tissue screening revealed the critical concentration of glyphosate to be 10 mg ml^-1 , and the critical concentration of antibiotic is 250 mg ml^-1 . Using this combination of glyphosate and antibiotic resulted in regenerated plants. This study established the optimal conditions for immature embryo callus tissue induction in maize.     

In vitro Culture of Abscissed Immature Avocado Embryos

The efficiency of an avocado hybridization programme, usingsmall potted glasshouse plants, was reduced by a high rate ofabscission of immature fruitlets bearing embryos too young forconventional germination. This was overcome in part by culturein vitro of the shed embryos on a liquid medium supplementedwith 0.5 mg l^–1 benzyladenine. Most embryos younger than6 weeks did not survive in culture, but older embryos slowlyproduced multiple shoots, with axillary shoot growth being furtherstimulated by removal of both cotyledons. Embryo response wasnot related to cultivar. Shoots removed from culture could begrafted to seedling rootstocks. Grafting was considered morereliable than dependence on growth of the main root or adventitiousroots in vitro to produce established plants. Persea americana Miller, avocado, abscissed fruitlets, in vitro embryo culture     

Plant Regeneration from Cultured Immature Embryos and Inflorescences of 25 Cultivars of Wheat (Triticum aestivum)

Immature embryos and inflorescences of wheat (Triticum aestivum)were cultured on agar media. The presence of the auxin 2, 4-Dinduced the formation of multiple shoots in a proportion ofcultures. In some cases the shoots developed from organized,embryoid-like structures. The morphogenetic capacities of 25different cultivars of spring and winter wheat were compared,and clear differences were found between genotypes. Shoots werecultured separately, and the resulting plants grown to maturityin the glasshouse. Over a thousand plants were regenerated inthis way, and phenotypic variation was observed amongst theregenerants. Key words: Wheat, Somatic embryogenesis, Regeneration     

小麦幼胚培养高效成株系统的建立

研究探讨了不同的基因型、幼胚取材时期、4℃处理时间、盾片接种方式、分化及生根条件等对小麦幼胚培养再生成株特性的影响,并在此基础上建立了一套高效、可靠、重复性好的小麦组培再生系统。优化条件下,该系统从幼胚诱导致密愈伤组织的频率为89％,致密愈伤组织的分化频率诱导2周时为95％,培养近3个月时仍可达50％以上。此外还发现部分叶状结构当转至新鲜的分化培养基上时能够进一步发育成为芽苗。分化的芽苗在生根培养基上大多生成丛生苗。从基部切开后,每棵芽苗／分蘖均可独立成株。组培苗均可正常地开花结实。     

In vitro Culture of Immature Embryos of Cytisus laburnum

Immature embryos of Cytisus laburnum L. were cultivated in vitro and four culture media, different techniques of substrate preparation, sucrose concentration and the effect of suspensor removal were tested. The best results were obtained with N6 medium supplemented with 2 mg dm⁻³ glycine and set up using a double-layer culture system, in which the top layer had a higher osmotic potential than the bottom one. These conditions allowed normal embryogenic development in up to 45 % of early globular embryos, that were able to develop until a complete maturity. Osmotic potential and mineral nutrients of the medium demonstrated to be crucial for the successful culture and their effects were dependent on embryo age at the time of excision. The presence of an intact suspensor showed to be beneficial only for early globular embryos while older developmental stage embryos were not significantly affected. This revised version was published online in July 2006 with corrections to the Cover Date.     

山橙胚的离体培养和植株再生

1植物名称 山橙（Melodinus suaveolens Champ．ex Benth．），又名马骝藤、马骝橙藤、猴子果。     

核桃离体胚的植株再生

在选育核桃优良单株时一般利用实生苗繁殖,由于自然杂交,常易丧失优良品种的性状。核桃扦插不易生根,嫁接繁殖因接口处易发生氧化褐变,因而成活率较低。为了利用组织培养技术进行核桃的快速无性繁殖,我们(1984)曾从核桃的不同