共查询到20条相似文献,搜索用时 15 毫秒
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Shirasugi I Sakakibara Y Yamasaki M Nishiyama K Matsui T Liu MC Suiko M 《Bioscience, biotechnology, and biochemistry》2010,74(11):2253-2258
Measurement of the melanin content by using B16 melanoma cells is generally applied to find novel skin-whitening agents. However, this measurement method using B16 melanoma cells has such disadvantages, as the time taken, its sensitivity, and troublesomeness. We therefore attempted in the present study to establish a reporter assay system by measuring the tyrosinase promoter activity to use for convenient, high-throughput screening of new melanogenesis inhibitors. We first confirmed the validity of this reporter assay system by using such known skin-whitening agents, as arbutin, sulforaphane, and theaflavin 3,3'-digallate. We then compared the effect of 56 compounds on the tyrosinase promoter activity to test this reporter assay system. Carnosol, and rottlerin strongly inhibited the tyrosinase promoter activity. Moreover, carnosol and rottlerin decreased melanin synthesis and tyrosinase expression in a dose-dependent manner when using B16 melanoma cells. These results indicate this new luciferase reported assay system to be an effective and convenient method for screening potential skin-whitening compounds. 相似文献
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Hiroyuki Horitsu Kiyohito Nakashima Mikio Tomoyeda 《Bioscience, biotechnology, and biochemistry》2013,77(11):2253-2254
Measurement of the melanin content by using B16 melanoma cells is generally applied to find novel skin-whitening agents. However, this measurement method using B16 melanoma cells has such disadvantages, as the time taken, its sensitivity, and troublesomeness. We therefore attempted in the present study to establish a reporter assay system by measuring the tyrosinase promoter activity to use for convenient, high-throughput screening of new melanogenesis inhibitors. We first confirmed the validity of this reporter assay system by using such known skin-whitening agents, as arbutin, sulforaphane, and theaflavin 3,3′-digallate. We then compared the effect of 56 compounds on the tyrosinase promoter activity to test this reporter assay system. Carnosol, and rottlerin strongly inhibited the tyrosinase promoter activity. Moreover, carnosol and rottlerin decreased melanin synthesis and tyrosinase expression in a dose-dependent manner when using B16 melanoma cells. These results indicate this new luciferase reported assay system to be an effective and convenient method for screening potential skin-whitening compounds. 相似文献
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Gpnmb is a melanoblast-expressed, MITF-dependent gene 总被引:1,自引:0,他引:1
Loftus SK Antonellis A Matera I Renaud G Baxter LL Reid D Wolfsberg TG Chen Y Wang C;NISC Comparative Sequencing Program Prasad MK Bessling SL McCallion AS Green ED Bennett DC Pavan WJ 《Pigment cell & melanoma research》2009,22(1):99-110
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Young Sic Eom Dongho Jeong A-Reum Ryu Keon-Hyoung Song Dai Sig Im Mi-Young Lee 《Current issues in molecular biology》2022,44(8):3312
Daphne odora, a blooming shrub, has been traditionally used for various medicinal purposes. However, information on its anti-melanogenic activity and dermal application is limited. In this study, the Daphne odora extract (DOE), with constituents including daphnetin, was used to investigate depigmenting activity and the underlying mechanism of Daphne odora. DOE inhibited in vitro and cellular tyrosinase activity in a dose-dependent manner, and reduced the α-MSH-induced melanin biosynthesis to a control level. The protein expressions of melanin synthesis-related enzymes were also significantly reduced by DOE. Moreover, DOE decreased the phosphorylation of cAMP-response element binding proteins (CREBs) induced by α-MSH in B16F10 cells, while it activated phosphorylated extra-cellular signal-regulated kinases (ERKs) and protein kinase B (AKT) expression. These results suggest that DOE might inhibit the melanogenesis signaling pathways by activating ERK- and AKT-signaling pathways to regulate the expression of CREB and MITF and its downstream pathways. Therefore, DOE could potentially be developed as a depigmenting agent. 相似文献
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Dickkopf-3(DKK3),Wnt/p-catenin信号通路中一个重要的抑制因子,可能参与调控黑色素生成过程.本文研究了DKK3在羊驼黑色素细胞中黑色素生成的作用.在羊驼黑色素细胞中,过表达DKK3显著下调Wntl,Lefl,Myc和黑色素生成相关基因MITF及其下游基因TYR,TYRP1和TYRP2的表达,在... 相似文献
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A novel synthetic Piper amide derivative NED‐180 inhibits hyperpigmentation by activating the PI3K and ERK pathways and by regulating Ca2+ influx via TRPM1 channels
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Eunson Hwang Taek Hwan Lee Wook‐Joo Lee Won‐Sik Shim Eui‐Ju Yeo Sanghee Kim Sun Yeou Kim 《Pigment cell & melanoma research》2016,29(1):81-91
Piper amides have a characteristic, unsaturated amide group and exhibit diverse biological activities, including proliferation and differentiation of melanocytes, although the molecular mechanisms underlying its antimelanogenesis effect remain unknown. We screened a selected chemical library of newly synthesized Piper amide derivatives and identified (E)‐3‐(4‐(tert‐butyl)phenyl)‐N‐(2,3‐dihydrobenzo[b][1,4]dioxin‐6‐yl)acrylamide (NED‐180) as one of the most potent compounds in suppressing melanogenesis. In murine melan‐a melanocytes, NED‐180 downregulated the expression of melanogenic regulatory proteins including tyrosinase, Tyrp1, Dct, and MITF. PI3K/Akt‐dependent phosphorylation of GSK3β by NED‐180 decreases MITF phosphorylation and inhibits melanogenesis without any effects on cytotoxicity and proliferation. Furthermore, topical application of NED‐180 significantly ameliorated UVB‐induced skin hyperpigmentation in guinea pigs. Interestingly, data obtained using calcium imaging techniques suggested that NED‐180 reduced the TPA‐induced activation of TRPM1 (melastatin), which could explain the NED‐180‐induced inhibition of melanogenesis. All things taken together, NED‐180 triggers activation of multiple pathways, such as PI3K and ERK, and inhibits TRPM1/TRPV1, leading to inhibition of melanogenesis. 相似文献
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Youn-Ho Shin Young-Kwon Seo Hee-Hoon Yoon Kye-Yong Song Jung-Keug Park 《Biotechnology and Bioprocess Engineering》2012,17(1):203-210
Melanocytes are the melanin-producing cells by melanogenesis, and the pigment melanin is primarily responsible for the color
of skin. These cells contain dendrites that are in close contact with neighboring keratinocytes. Keratinocytes produce and
secrete factors that regulate the proliferation and melanogenesis of melanocytes in vitro. Therefore, adopting only melanocyte pure culture may not clearly reflect the skin physiology in vivo. In this study, we applied a two-culture model using melanocytes and keratinocytes from human skin, such as melanocyte pure
culture and melanocyte co-culture with keratinocyte. And then, there was compared the responses of melanocytes under different
culture conditions (treatment with arbutin, MSH-α and UV-B irradiation). The results show that there was no significant difference
in melanocyte proliferation and melanogenesis between arbutin and MSH-α treatment. However, the co-culture model was more
stable than the pure culture model in terms of melanocyte proliferation and melanogenesis upon UV-B irradiation. Therefore,
the co-culture model was superior to the pure culture as a useful method for the study of melanocytes and epidermal melanin
unit. 相似文献
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