Architecture of a dodecameric bacterial replicative helicase

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Highlights? Crystal structure of the ATPase domain of DnaB from Helicobacter pylori ? MALLS analysis shows that HpDnaB forms dodecamers ? EM reconstruction shows that HpDnaB forms head-to-head double hexamers     

The bacterial stressosome: a modular system that has been adapted to control secondary messenger signaling

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Highlights? Regulation of cyclic di-GMP biosynthesis in Moorella thermoacetica ? Stressosome signal transduction and resetting ? X-ray crystal structure of type 2C phosphatase MtX ? X-ray crystal structures of phosphorylated MtS and the N-terminal domain of MtR     

The Structure of the Tiam1 PDZ Domain/ Phospho-Syndecan1 Complex Reveals a Ligand Conformation that Modulates Protein Dynamics

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Highlights? The Tiam1 PDZ domain preferentially binds a subset of syndecan (SDC) proteins ? The PDZ domain/phospho-SDC1 structure reveals a phosphoryl binding pocket ? Ligand specificity and phosphorylation regulate PDZ/SDC interactions and signaling ? Tiam1 PDZ domain dynamics are ligand-dependent and regulated by phosphorylation     

Design of a Phosphorylatable PDZ Domain with Peptide-Specific Affinity Changes

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Highlights? Successful design of phosphorylation sites into a globular protein ? Redesigned PDZ domain phosphorylation is associated with local destabilization ? Phosphorylation switches peptide binding function ? Phosphorylation switches peptide recognition specificity     

Targeting the Cell Wall of Mycobacterium tuberculosis: Structure and Mechanism of L,D-Transpeptidase 2

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Highlights? The structure of Mtb Ldt_Mt2 shows the binding of a peptidoglycan fragment ? The structure of the complex maps the interaction between acyl donor and enzyme ? Ldts show a conserved C-terminal ykuD domain with a divergent N-terminal domain ? Based on the structure of this complex, a reaction mechanism is proposed     

Structural and functional analysis of HtrA1 and its subdomains

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Highlights? IGFBP-like domain structure suggests that it is incompetent for IGF binding ? Kazal-like domain structure shows distorted “P1” loop incompetent for inhibition ? Protease domain shows catalytic readiness in apo form ? SAXS envelope for full-length HtrA1     

Structural Determinants of RGS-RhoGEF Signaling Critical to Entamoeba histolytica Pathogenesis

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Highlights? E. histolytica expresses a convergently evolved RGS-RhoGEF protein ? EhRGS-RhoGEF possesses a conventional nine-helix RGS domain ? Activated EhRGS-RhoGEF induces S2 cell spreading through Rac1/2 ? EhRGS-RhoGEF modulates amoebic migration and host cell killing     

The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain

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Highlights? ESCRT-I subunit UBAP1 is essential for degradation of antiviral protein tetherin ? UBAP1 has a domain consisting of a solenoid of overlapping UBAs (SOUBA) ? Each of the three UBAs in the SOUBA binds monoubiquitin     

Disease mutations in the ryanodine receptor central region: crystal structures of a phosphorylation hot spot domain

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Highlights? Crystal structures of a central RyR domain contain phosphorylation sites ? Eleven RyR1 disease mutations cluster on two opposite faces of the domain ? The RyR1 L2867G mutation causes a drastic destabilization ? In vitro phosphorylation identifies 5 PKA and 4 CaMKII targets     

Adaptor-dependent degradation of a cell-cycle regulator uses a unique substrate architecture

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Highlights? PdeA degradation requires an N-terminal PAS domain and a C-terminal degron ? CpdR acts as an adaptor to tether PdeA to the ClpXP protease ? One subunit of PdeA can deliver its partner subunit via PAS dimerization ? Separation of adaptor and protease recognition sites promotes processive degradation     

Zinc Drives a Tertiary Fold in the Prion Protein with Familial Disease Mutation Sites at the Interface

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Highlights? Zinc binds to the prion protein octarepeat domain ? The zinc-bound octarepeat makes a tertiary contact to C-terminal helices 2 and 3 ? The encompassed surface carries a majority of the mutations in familial prion disease ? Familial mutations weaken this zinc-driven tertiary contact     

Sprouty1 Regulates Reversible Quiescence of a Self-Renewing Adult Muscle Stem Cell Pool during Regeneration

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Highlights? Pax7⁺ skeletal satellite cells regenerate muscle in their endogenous environment ? Quiescent satellite cells express the RTK inhibitor Spry1 ? Spry1 is needed for satellite cells to return to quiescence after muscle injury ? Deletion of Spry1 in satellite cells depletes the muscle stem cell pool     

ZNF750 is a p63 target gene that induces KLF4 to drive terminal epidermal differentiation

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Highlights? Induction of ZNF750 expression is necessary for terminal epidermal differentiation ? The C2H2 domain of ZNF750 is necessary for its prodifferentiation function ? p63 is necessary for the induction of ZNF750 expression during differentiation ? ZNF750 activates the expression of KLF4 to drive differentiation gene expression     

Legionella pneumophila Effector RomA Uniquely Modifies Host Chromatin to Repress Gene Expression and Promote Intracellular Bacterial Replication

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Highlights? Upon infection, L. pneumophila secretes RomA, a SET domain-containing methyltransferase ? RomA triggers H3K14 trimethylation, causing a switch from gene activation to repression ? ChIP-seq identified 4,870 H3K14 methylated promoter regions, including innate immune genes ? RomA SET domain is required for efficient intracellular replication of L. pneumophila     

Characterization and In Vivo Pharmacological Rescue of a Wnt2-Gata6 Pathway Required for Cardiac Inflow Tract Development

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Highlights? Wnt2 is required for atrial and inflow tract morphogenesis ? Wnt2 regulates expansion of secondary heart field progenitors ? Defects in Wnt2^?/? mutants can be rescued using Wnt signaling agonists ? Wnt2 cooperates with Gata6 to regulate cardiac inflow tract development     

Conformational Dynamics of the Rpt6 ATPase in Proteasome Assembly and Rpn14 Binding

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Highlights? Proteasome ATPase Rpt6 undergoes helix-coil exchange in its C-terminal domain ? Rpt6 G^360,387A has a stabilized four-helix bundle and raised melting temperature ? Assembly chaperone Rpn14 binds selectively to the four-helix bundle Rpt6 conformer ? Rpt6 G^360,387A (rpt6AA) is synthetically defective with an rpn14 null mutation     

Myosin-IXA regulates collective epithelial cell migration by targeting RhoGAP activity to cell-cell junctions

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Highlights? Myosin-IXA, an actin-motor- and RhoGAP-protein, is required for collective migration ? Myosin-IXA is required for radial actin bundle assembly at nascent cell-cell contacts ? The localization of myosin-IXA to actin bundles is dependent on its motor domain ? Myosin-IXA RhoGAP domain locally inhibits Rho stabilizing nascent cell-cell contacts     

Moonlighting by different stressors: crystal structure of the chaperone species of a 2-Cys peroxiredoxin

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Highlights? The Schistosoma mansoni peroxiredoxin I (SmPrxI) is a moonlighting protein ? SmPrxI switches from LMW peroxidase to HMW chaperone due to chemical stressors ? The three-dimensional structures of both LMW and HMW SmPrxI have been solved ? SmPrxI HMW is the structure of a Prx chaperone     

Crystal structures of the outer membrane domain of intimin and invasin from enterohemorrhagic E. coli and enteropathogenic Y. pseudotuberculosis

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Highlights? First intimin or invasin β domain structure—both crystallize as monomers ? First structure solved using 3-λ SeMet MAD data from crystals grown in LCP ? Identification of a non-BIG domain directly downstream of the β domain ? Highly conserved and coevolving residues are identified in the Int/Inv family