共查询到20条相似文献,搜索用时 25 毫秒
1.
Zblkowska E 《The Journal of parasitology》2003,89(1):76-79
The objective of this work was to examine the calcium concentration in the shells of naturally infected individuals of Lymnaea stagnalis. Calcium concentration in the water of lakes and in snail shells was examined by the EDTA method. Calcium concentration in the shells of infected snails was found to be higher than that in the shells of uninfected ones only in the lake with the lowest calcium concentration in water. However, this difference is not evidence of hypercalcification of snail shells caused by trematode larvae but suggests that the limited calcium concentration in the environment can be a very good factor for studying snail shell calcification under natural conditions. Moreover, small adult snails from 2 lakes had more calcium in their shells than did large ones. Different reproductive rates of different-sized snails could be the cause of this phenomenon. 相似文献
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Dr. Benita Plesch 《Cell and tissue research》1976,171(3):389-396
The attachment of the body of the snail Lymnaea stagnalis to the shell was studied by histochemistry and light and electron microscopy. Muscles of the body wall insert into the connective tissue by way of long thin projections of sarcolemma. The muscle cells end under the basement membrane of a specialised area of the epidermis, the adhesive epithelium. The cells of this epithelium are filled with microfilaments and possess characteristic knob-like microvilli. The epithelium is attached to the shell by way of an adhesive substance containing proteins and mucopolysaccharides.This research was made possible by a grant from the Netherlands Organization for Pure Research (Z.W.O.) 相似文献
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Marijke de Jong-Brink H. M. Koop W. F. de Roos J. M. Bergamin-Sassen 《Invertebrate reproduction & development.》2013,57(4):207-219
The secretory activity of the albumen gland of the freshwater snail Lymnaea stagnalis was studied morphometrically (both at the light- and at the electron-microscope level) and biochemically under the following experimental conditions: (1) glandular tissue was implanted into acceptor snails and the glandular activity of the implants was compared to that of the glands of the donors and acceptors; (2) glandular activity was measured at various periods after oviposition; and (3) the activity was measured during a 24 h cycle (diurnal activity). The results indicate that cellular release of secretion material is regulated by a nervous mechanism, whereas synthetic activity is under hormonal control. 相似文献
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Kamila Dmochowska Katarzyna Kamińska Regina Frączek Krystyna Żółtowska Elżbieta Żbikowska 《Biologia》2013,68(2):278-287
Lymnaea stagnalis is an intermediate host of many Digenea. The infestation affects host metabolism. The aim of the work was to investigate hemolymph biochemical indicators of L. stagnalis infected with four species of trematodes: Diplostomum pseudospathaceum, Paryphostomum radiatum, Plagiorchis elegans or Opisthioglyphe ranae. The protein profiles and proteinase activity in the hemolymph of sexually mature individuals of Lymnaea stagnalis maintained at 19°C were tested. As the carbohydrates are main substrates for energetic metabolism of the great pond snail their content and disaccharidase activity were also studied. Hemolymph samples were collected during weeks 3 and 4 of rearing. No significant differences in the total protein content between uninfected individuals and snails infected with the first three trematode species were detected. In the snails infected with O. ranae the quantity of total proteins was near twice higher than in those uninfected. A higher share of 70 kDa proteins in infected than in uninfected snails as well as reduction of the low molecular weight fractions of proteins for snails infected with D. pseudospathaceum and P. radiatum were detected. During week 3, carbohydrate content in the infected snails did not differ from that in the controls while during week 4 it was significantly lower in snails infected with P. elegans or O. ranae. The content of the major soluble carbohydrate in the hemolymph - saccharose — changed in a similar way. No activity of trypsin or pepsin in the hemolymph sample was detected while the activity of chymotrypsin was lower in infected snails vs. controls. On the other hand, saccharase and maltase activities were higher in infected than in uninfected snails. The biochemical hemolymph indicators in naturally infected host-snails show some differences depending on the parasite species but they are not sufficiently species-specific to offer the basis for establishing the model unique for a particular parasitosis. Our results from the field did not always coincide with those from the laboratory. 相似文献
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Vyskushenko DA 《Parazitologiia》2001,35(4):320-324
The influence of different concentrations of the copper sulfate (0.2, 1, 1.8 mg/l) on the Lymnaea stagnalis heart rate in a control and in the case of the trematode infection was examined. Concentrations which cause the decrease and the increase of heart rate were estimated. Pathological reactions in infected individuals are more expressed and appear earlier. 相似文献
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McKENZIE J. DOUGLAS Caunce Maria Hetherington Mark S. Winlow William 《Brain Cell Biology》1998,27(6):459-470
The aminergic innervation of the foot of Lymnaea stagnalis was investigated using electron microscopy, immunocytochemistry, and HPLC. The foot was found to contain large amounts of serotonin and dopamine, though at lower concentrations than are found in nervous tissue. Serotonin containing tissue was concentrated in the ventral surface of the foot, under ciliated areas of the epidermis where it occurred in varicosities, with fine tracts joining these varicosities. Varicosities also occurred in deeper tissues, probably adjacent to mucus cells. Positive fluorescence for serotonin in axons was found in nerves innervating the foot, but few neuronal cell bodies containing serotonin were detected, indicating that most of the innervation was coming from the central ganglia. Axon varicosities were found using TEM on ciliated cells, mucus cells, and muscle cells as well as interaxonal junctions (possibly non-synaptic) within nerves. The neuronal varicosities contacting the ciliated cells and mucus cells contained mostly dense-cored vesicles of between 60 and 100 nm in diameter. Smaller, lucent vesicles also occurred in these terminals. The origin and significance of this innervation is discussed. It is suggested that both serotonin and dopamine may play a large role in controlling ciliary gliding by the foot. 相似文献
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We assessed how spatial and temporal heterogeneity and competition structure larval trematode communities in the pulmonate snail Lymnaea stagnalis . To postulate a dominance hierarchy, mark-release-recapture was used to monitor replacements of trematode species within snails over time. In addition, we sampled the trematode community in snails in different ponds in 3 consecutive years. A total of 7,623 snails (10,382 capture events) was sampled in 7 fishponds in the Jind?ich?v Hradec and T?eboň areas in South Bohemia (Czech Republic) from August 2006 to October 2008. Overall, 39% of snails were infected by a community of 14 trematode species; 7% of snails were infected with more than 1 trematode species (constituting 16 double- and 4 triple-species combinations). Results of the null-model analyses suggested that spatial heterogeneity in recruitment among ponds isolated trematode species from each other, whereas seasonal pulses in recruitment increased species interactions in some ponds. Competitive exclusion among trematodes led to a rarity of multiple infections compared to null-model expectations. Competitive relationships among trematode species were hypothesized as a dominance hierarchy based on direct evidence of replacement and invasion and on indirect evidence. Seven top dominant species with putatively similar competitive abilities (6 rediae and 1 sporocyst species) reduced the prevalence of the other trematode species developing in sporocysts only. 相似文献
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Kononenko NL Zhukov VV 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2005,91(3):300-308
Presence of GABA-immunoreactive fibres in the nerve cervicalis inferior was shown as well as their regulatory effects upon contractions of the dorsal longitudinal muscle in Lymnaea stagnalis. The exogenous GABA affected the amplitude of evoked muscle contractions diminishing them at concentrations from 10(-8) M and augmenting them from above 10(-5) M. Picrotoxin produced opposite effects on the muscle contractions in the same concentration range. Bicucullin did not affect the contractions. The findings are discussed from the standpoint of the supposed functional heterogeneity of the GABA receptors in the mollusc nerve-muscle junction. 相似文献
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Summary High performance liquid chromatography has been used to identify the eicosanoids produced from radiolabelled arachidonic acid by accessory sex organs of Lymnaea stagnalis. Radiolabelled compounds co-eluting with the primary prostaglandins normally associated with invertebrates were present, together with two eicosanoids known to occur in vertebrates. These results lend support to the suggestion of van Duivenboden (Int. J. Invertebr. Reprod., 6 (1983) 249–257) that prostaglandins (PGs) are involved in accelerating the onset of egg laying in female copulants of this species. The inability of indomethacin and aspirin to completely inhibit PG biosynthesis in L. stagnalis suggests that the PG synthetase of this snail differs from that of mammals and most other invertebrates. 相似文献
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D Girdlestone C R McCrohan W Winlow 《Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol.》1989,93(2):333-339
1. Cerebral giant cells (CGCs) in the isolated central nervous system (CNS) of the pond snail Lymnaea stagnalis (L.) exhibit bursting activity when superfused with anaesthetic concentrations of halothane. 2. Calcium-dependent components of the CGC action potential appear more sensitive to halothane than do other ionic mechanisms. 3. Higher concentrations of halothane block the chemical synaptic connection between CGC and buccal motoneurone B1, but have no effect on the strong electrotonic coupling between the CGCs. 4. The mechanisms underlying CGC bursting and synaptic block in the presence of halothane are discussed. 相似文献
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《Comparative biochemistry and physiology. A, Comparative physiology》1981,68(2):199-209
- 1.1. Double intracellular and extracellular recordings from cell bodies and axons were made to study the interactions between the neurosecretory “Light Yellow” bursting pacemaker cells (LYC) in the right parietal ganglion of Lymnaea stagnalis.
- 2.2. The LYC are interconnected by low efficiency, non-rectifying electrotonic junctions, transmitting low frequencies only.
- 3.3. Often bursts in different cells coincide; apparently the junctions are responsible for this coherence.
- 4.4. It is inferred that the coupling serves to bring about a pulse-wise release of the cluster's secretory product.
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The effects of lead (5 or 10 ppm) on the survival of the freshwater snail Lymnaea stagnalis (L.) collected from lead contaminated or uncontaminated environments were evaluated under controlled laboratory conditions.
The animals from the contaminated environment had significantly greater survivability than those from the unpolluted environment
to subsequent acute (up to 24 days) exposure to lead. Acute (72 h) exposure to lead inhibited several behavioural activities
including locomotion, feeding, tentacle extension and emergence from the shell. Lead bioaccumulated in the snail tissues,
especially the buccal mass and stomach. The freshwater snail provides a valuable system for studying the bioaccumulation and
development of tolerance to environmental lead.
Electronic Publication 相似文献
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E. W. Roubos 《Cell and tissue research》1975,160(3):291-314
The process of neurosecretion in the Caudo-Dorsal Cells (CDC) of the freshwater snail Lymnaea stagnalis, which produce an ovulation hormone, shows a diurnal rhythmicity. Synthesis, transport and release of the neurosecretory material (NSM) is high during the evening and the early night and low during the rest of the day, while storage of NSM mainly occurs during the daytime. In the present study the role of the eyes in the regulation of the CDC-rhythm was investigated. During a 24-hr period, at time intervals of 6 hrs, cerebral ganglia, which contain CDC, of blinded and control snails (5 per group) were fixed and the CDC were studied with quantitative electron microscopical methods. The CDC of the controls showed a distinct diurnal thythmicity. Blinding, on the other hand, clearly affected this rhythmicity. The results indicate that after blinding the circadian CDC-rhythms of individual snails are no longer synchronous with each other ("interanimal desynchronization"). It is suggested that the rhythm of CDC neurosecretory activity is synchronized by the natural light/dark cycle via the eyes. The information from the eyes probably reaches the CDC via a nervous pathway. True snyapses and three types of synapse-like structures were found on the CDC. Their role in the regulation of CDC-activity is discussed. The effect of blinding is specific for the CDC; blinding does not influence the diurnal rhythmicity of another type of cerebral neurosecretory cells, the Light Green Cells (LGC). The CDC within a cluster act synchronously. This synchrony does not depend upon the presence of the eyes. Some structures which may be involved in establishing this synchrony, such as subsurface cisterns, desmosome-like structures and "specific release sites", are described. 相似文献
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M Kyriakides C R McCrohan C T Slade N I Syed W Winlow 《Comparative biochemistry and physiology. A, Comparative physiology》1989,93(4):861-876
1. A morphological and electrophysiological map of the identifiable neurones and neuronal clusters of the paired pedal ganglia has been prepared. 2. Neuronal morphology was investigated using the fluorescent dye, Lucifer Yellow CH, whilst electrophysiological properties were studied using conventional intracellular recording techniques and the phase plane technique. 3. The paired pedal ganglia are largely symmetrical and giant neurones usually have contralateral homologues. 4. Neuronal clusters are also paired, but minor asymmetries, both of identifiable neurones and neuronal clusters have been found to exist. 5. These asymmetries are thought to be related to asymmetries of body form. 6. Most of the individually identifiable neurones possess obligatory axon branches which are invariant from one preparation to the next, but variant branches also occur. 7. Within the neuronal clusters, morphology appears to be more variable. 8. Individually identifiable neurones and neuronal clusters were characterized electrophysiologically according to the criteria of action potential shape, spontaneous activity pattern, electrical coupling and common synaptic inputs. 9. Homologous pairs of neurones usually have similar electrophysiological properties, as do those within clusters. 10. A number of wide-acting synaptic inputs have been identified on neurones of the pedal, buccal, visceral and parietal ganglia. 相似文献
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E. W. Roubos 《Cell and tissue research》1976,168(1):11-31
The cerebral ganglia of the freshwater snail Lymnaea stagnalis contain two clusters of neurosecretory Caudo-Dorsal Cells (CDC). These cells produce a neurohormone which stimulates ovulation. Ganglion transplantation and quantitative electron microscopy show that neuronal isolation of the cerebral ganglia complex (CCC) results in an activation of the CDC. It was, therefore, concluded that the CDC are controlled by an inhibitory neuronal input originating outside the cerebral ganglia. Ultrastructural studies on synaptic degeneration in the CCC suggest that this input reaches the CDC via a special type of synapse-like structure, the type C-SLS.Furthermore, transplantation of CCC into acceptor snails leads to a reduced release and an increased intracellular breakdown of neurohormone in the CDC of the nervous system of the acceptors. It is supposed that these phenomena are caused by the release of an (unknown) factor from the transplanted CCC. Special attention was given to the formation and degradation of a peculiar type of neurohormone granule, the large electron dense granule. 相似文献
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