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1.
Zlatskaia AV 《Genetika》2005,41(8):1013-1026
The results of studies on genetic control of the grain protein content character in common wheat and the attempts of its improvement using traditional breeding approaches and methods of experimental mutagenesis and introgressive hybridization are presented. The evolution of the views on the genetic structure of the character is considered. Possible explanations are given to the fact that the attempts to significantly improve the character in question in common wheat cultivars have failed in spite of long-term genetic and selection studies. Possible lines of further investigation of genetic control of grain protein content and its increase in common wheat are discussed. 相似文献
2.
The phenotypic manifestation and genetic control of embryo lethality observed in crosses between common wheat and rye were studied. It was found that crosses between common wheat and inbred self-fertile rye lines L2 and 535 gave rise to ungerminating grains, in which the development and differentiation of the hybrid embryo are arrested. Study of the degree of embryo development in the hybrid grains obtained by crossing common wheat varieties with inbred rye lines L2 and 535 showed that genotypes of the parents affected the ratio between undifferentiated embryos of various sizes. Analysis of this trait was performed by test crosses according to a novel pedigree program with the use of interlinear hybrids and a set of fourth-generation hybrid recombinant inbred lines. Rye line L2 was shown to bear the Eml (Embryo lethality) gene, which terminates the development of the hybrid embryo in amphihaploids. The suggestion of complementary interaction between wheat and rye genes during formation of a “new” character in wheat-rye F1 hybrids is discussed. A method of detecting an allele not complementary to the rye Eml allele in wheat is proposed. The proposed test program allows appropriate study of the system of wheat and rye genes involved in complementary interaction in the genotype of a distant hybrid.__________Translated from Genetika, Vol. 41, No. 8, 2005, pp. 1075–1083.Original Russian Text Copyright © 2005 by Tikhenko, Tsvetkova, Voylokov. 相似文献
3.
The results of the hybrid and monosomic analyses of smoky ear coloration in the common wheat variety columbina are reported. The character has been found to be monogenic, and its gene has been located to chromosome 1DS. The smoky ear coloration is determined by an allele of gene Rg2 introgressed to wheat from Aegilops tauschii.__________Translated from Genetika, Vol. 41, No. 8, 2005, pp. 1147–1149.Original Russian Text Copyright © 2005 by Pshenichnikova, Bokarev, Shchukina. 相似文献
4.
M. Rapp V. Lein F. Lacoudre J. Lafferty E. Müller G. Vida V. Bozhanova A. Ibraliu P. Thorwarth H. P. Piepho W. L. Leiser T. Würschum C. F. H. Longin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(6):1315-1329
Key message
Simultaneous improvement of protein content and grain yield by index selection is possible but its efficiency largely depends on the weighting of the single traits. The genetic architecture of these indices is similar to that of the primary traits.Abstract
Grain yield and protein content are of major importance in durum wheat breeding, but their negative correlation has hampered their simultaneous improvement. To account for this in wheat breeding, the grain protein deviation (GPD) and the protein yield were proposed as targets for selection. The aim of this work was to investigate the potential of different indices to simultaneously improve grain yield and protein content in durum wheat and to evaluate their genetic architecture towards genomics-assisted breeding. To this end, we investigated two different durum wheat panels comprising 159 and 189 genotypes, which were tested in multiple field locations across Europe and genotyped by a genotyping-by-sequencing approach. The phenotypic analyses revealed significant genetic variances for all traits and heritabilities of the phenotypic indices that were in a similar range as those of grain yield and protein content. The GPD showed a high and positive correlation with protein content, whereas protein yield was highly and positively correlated with grain yield. Thus, selecting for a high GPD would mainly increase the protein content whereas a selection based on protein yield would mainly improve grain yield, but a combination of both indices allows to balance this selection. The genome-wide association mapping revealed a complex genetic architecture for all traits with most QTL having small effects and being detected only in one germplasm set, thus limiting the potential of marker-assisted selection for trait improvement. By contrast, genome-wide prediction appeared promising but its performance strongly depends on the relatedness between training and prediction sets.5.
Allopolyploidy in Wheat Induces Rapid and Heritable Alterations in DNA Methylation Patterns of Cellular Genes and Mobile Elements 总被引:2,自引:0,他引:2
Y. Z. Dong Z. L. Liu X. H. Shan T. Qiu M. Y. He B. Liu 《Russian Journal of Genetics》2005,41(8):890-896
Whereas accumulating recent evidences indicate that allopolyploid formation in plants is accompanied by rapid and non-Mendelian genomic changes, some other works showed genomic stasis in both nascent and natural allopolyploids. To further study the issue, we performed global DNA fingerprinting of a newly synthesized allohexaploid wheat and its natural counterpart, the common wheat, by AFLP analysis. It was found that ca. 20% bands showed deviation from parental additivity in both synthetic and natural common wheat. Sequence analysis indicates that a majority of the changed bands represent known-function genes and transposable elements. DNA gel blot analysis showed that the main type of changes in the amphiploid is epigenetic in nature, i.e., alteration in DNA methylation patterns. Two types of alterations in methylation, random and non-random, were detected, and both types were stably inherited. Possible causes and implications of the epigenetic changes in allopolyploid genome evolution and speciation are discussed.__________From Genetika, Vol. 41, No. 8, 2005, pp. 1089–1095.Original English Text Copyright © 2005 by Dong, Liu, Shan, Qiu, He, Liu.This text was submitted by the authors in English. 相似文献
6.
A Genetic Framework for Grain Size and Shape Variation in Wheat 总被引:1,自引:0,他引:1
Vasilis C. Gegas Aida Nazari Simon Griffiths James Simmonds Lesley Fish Simon Orford Liz Sayers John H. Doonan John W. Snape 《The Plant cell》2010,22(4):1046-1056
Grain morphology in wheat (Triticum aestivum) has been selected and manipulated even in very early agrarian societies and remains a major breeding target. We undertook a large-scale quantitative analysis to determine the genetic basis of the phenotypic diversity in wheat grain morphology. A high-throughput method was used to capture grain size and shape variation in multiple mapping populations, elite varieties, and a broad collection of ancestral wheat species. This analysis reveals that grain size and shape are largely independent traits in both primitive wheat and in modern varieties. This phenotypic structure was retained across the mapping populations studied, suggesting that these traits are under the control of a limited number of discrete genetic components. We identified the underlying genes as quantitative trait loci that are distinct for grain size and shape and are largely shared between the different mapping populations. Moreover, our results show a significant reduction of phenotypic variation in grain shape in the modern germplasm pool compared with the ancestral wheat species, probably as a result of a relatively recent bottleneck. Therefore, this study provides the genetic underpinnings of an emerging phenotypic model where wheat domestication has transformed a long thin primitive grain to a wider and shorter modern grain. 相似文献
7.
Toi J. Tsilo Gary A. Hareland Senay Simsek Shiaoman Chao James A. Anderson 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(4):717-730
Kernel characteristics, particularly kernel weight, kernel size, and grain protein content, are important components of grain
yield and quality in wheat. Development of high performing wheat cultivars, with high grain yield and quality, is a major
focus in wheat breeding programs worldwide. Here, we report chromosome regions harboring genes that influence kernel weight,
kernel diameter, kernel size distribution, grain protein content, and grain yield in hard red spring wheat breeding lines
adapted to the Upper Midwest region of the United States. A genetic linkage map composed of 531 SSR and DArT marker loci spanned
a distance of 2,505 cM, covering all 21 chromosomes of wheat. Stable QTL clusters influencing kernel weight, kernel diameter,
and kernel size distribution were identified on chromosomes 2A, 5B, and 7A. Phenotypic variation explained by individual QTL
at these clusters varied from 5 to 20% depending on the trait. A QTL region on chromosome 2B confers an undesirable pleiotropic
effect or a repulsion linkage between grain yield (LOD = 6.7; R
2 = 18%) and grain protein content (LOD = 6.2; R
2 = 13.3%). However, several grain protein and grain yield QTL independent of each other were also identified. Because some
of the QTL identified in this study were consistent across environments, DNA markers will provide an opportunity for increasing
the frequency of desirable alleles through marker-assisted selection. 相似文献
8.
本研究了小麦籽粒蛋白质合量在Fl及F2代中的表现情况。结果表明,控制蛋白质合量的基因作用以加性效应为主,同时存在非加性效应;Fl代子粒蛋白质合量与双亲平均值高度相关,F2代中子粒蛋白质合量分离呈正态分布,且正态分布的峰值蛋白质合量也接近双亲平均值。蛋白质合量的一般配合力大于特殊配合力方差,并计算了蛋白质合量的广义和狭义遗传力。 相似文献
9.
10.
Some Swedish spring wheat varieties have recently been shown to carry a rare wildtype (wt) allele of the gene NAM-B1, known to affect leaf senescence and nutrient retranslocation to the grain. The wt allele is believed to increase grain protein concentration and has attracted interest from breeders since it could contribute to higher grain quality and more nitrogen-efficient varieties. This study investigated whether Swedish varieties with the wt allele differ from varieties with one of the more common, non-functional alleles in order to examine the effect of the gene in a wide genetic background, and possibly explain why the allele has been retained in Swedish varieties. Forty varieties of spring wheat differing in NAM-B1 allele type were cultivated under controlled conditions. Senescence was monitored and grains were harvested and analyzed for mineral nutrient concentration. Varieties with the wt allele reached anthesis earlier and completed senescence faster than varieties with the non-functional allele. The wt varieties also had more ears, lighter grains and higher yields of P and K. Contrary to previous information on effects of the wt allele, our wt varieties did not have increased grain N concentration or grain N yield. In addition, temporal studies showed that straw length has decreased but grain N yield has remained unaffected over a century of Swedish spring wheat breeding. The faster development of wt varieties supports the hypothesis of NAM-B1 being preserved in Fennoscandia, with its short growing season, because of accelerated development conferred by the NAM-B1 wt allele. Although the possible effects of other gene actions were impossible to distinguish, the genetic resource of Fennoscandian spring wheats with the wt NAM-B1 allele is interesting to investigate further for breeding purposes. 相似文献
11.
Pre-Anthesis Reserve Utilization for Protein and Carbohydrate Synthesis in Grains of Wheat 总被引:15,自引:0,他引:15
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We assessed the contribution of pre-anthesis reserve C to protein and carbohydrate deposition in grains of wheat (Triticum aestivum L.) using a new approach comprised of steady-state 13C/12C labeling and separation of the protein and carbohydrate fractions of mature grains. Experiments were performed with two spring wheat cultivars (Kadett and Star) grown with differential N fertilizer supply over 2 years. Pre-anthesis reserves contributed between 30% and 47% of the C in protein and 8% to 27% of the C in carbohydrates of grains. Partitioning of pre-anthesis C among the grain fractions was strongly dependent on the C/N (w/w) ratio in mobilized pre-anthesis biomass (r2 = 0.92). There appeared to be no significant exchange of pre-anthesis C between amino acids and carbohydrates during redistribution. The mean apparent efficiency of mobilized carbohydrate-C use in grain filling (MECHO, estimated as the mass of pre-anthesis C deposited in grain carbohydrates per gram of pre-anthesis C mobilized from carbohydrates in vegetative plant parts) was 0.72, whereas that of protein-C (MEP) was 0.56. However, MEP and MECHO varied among treatments. MECHO increased with increasing contributions of water-soluble carbohydrates to total pre-anthesis carbohydrate mobilization. MEP decreased with increasing residence time of protein in vegetative biomass. Possible causes for variability of MEP and MECHO are discussed. 相似文献
12.
防雨池栽条件下研究了花后干旱和渍水胁迫对两个不同品质类型小麦(Triticum aestivum)品种籽粒产量和品质形成的影响。结果表明,花后渍水和干旱处理明显降低了小麦籽粒产量和蛋白质产量。在整个灌浆期内干旱处理明显提高了籽粒蛋白质和醇溶蛋白含量,而渍水处理降低了籽粒蛋白质及其组分的积累量。籽粒总淀粉和直链淀粉含量以渍水处理最高,而支链淀粉以对照最高。干旱处理提高了籽粒干、湿面筋含量、沉降值和降落值,而渍水处理降低了上述品质指标。试验表明干旱和渍水胁迫对小麦籽粒蛋白质与淀粉的含量和组分及面粉品质等均有不同程度的影响,从而改变了不同品质类型小麦的籽粒品质。 相似文献
13.
The genetic control of grain hardness and its association with the specific friabilin content on starch granules of common wheat cultivars and lines with intervarietal substitutions of homeologous group-5 chromosomes were studied. A significant correlation was revealed between the technological parameters of grain hardness (mean size of flour particles) and the specific content of puroindolines on the starch surface estimated in terms of starch doses. The results obtained allowed the method of starch doses to be used to identify soft and hard wheat cultivars and lines based on an analysis of a single grain. The biochemical analysis confirmed the previously obtained estimates of flour-grinding properties of wheat cultivars and substitution lines and allowed specific genotypes to be characterized according to the composition of puroindolines. The influence of chromosomes 5D and 5A of donor wheat cultivars on the activity of the Ha loci of recipient cultivars was revealed and found to be associated with the composition of PIN products and with the expression of the Pina-D1 and Pinb-D1 genes. 相似文献
14.
Kunpu Zhang Junjun Wang Liyi Zhang Chaowu Rong Fengwu Zhao Tao Peng Huimin Li Dongmei Cheng Xin Liu Huanju Qin Aimin Zhang Yiping Tong Daowen Wang 《PloS one》2013,8(3)
Grain weight, an essential yield component, is under strong genetic control and markedly influenced by the environment. Here, by genome-wide association analysis with a panel of 94 elite common wheat varieties, 37 loci were found significantly associated with thousand-grain weight (TGW) in one or more environments differing in water and fertiliser levels. Five loci were stably associated with TGW under all 12 environments examined. Their elite alleles had positive effects on TGW. Four, two, three, and two loci were consistently associated with TGW in the irrigated and fertilised (IF), rainfed (RF), reduced nitrogen (RN), and reduced phosphorus (RP) environments. The elite alleles of the IF-specific loci enhanced TGW under well-resourced conditions, whereas those of the RF-, RN-, or RP-specific loci conferred tolerance to the TGW decrease when irrigation, nitrogen, or phosphorus were reduced. Moreover, the elite alleles of the environment-independent and -specific loci often acted additively to enhance TGW. Four additional loci were found associated with TGW in specific locations, one of which was shown to contribute to the TGW difference between two experimental sites. Further analysis of 14 associated loci revealed that nine affected both grain length and width, whereas the remaining loci influenced either grain length or width, indicating that these loci control grain weight by regulating kernel size. Finally, the elite allele of Xpsp3152 frequently co-segregated with the larger grain haplotype of TaGW2-6A, suggesting probable genetic and functional linkages between Xpsp3152 and GW2 that are important for grain weight control in cereal plants. Our study provides new knowledge on TGW control in elite common wheat lines, which may aid the improvement of wheat grain weight trait in further research. 相似文献
15.
Identification of the genetic controlling system of the protein content in the grain of winter wheat
L. L. Lysa 《Cytology and Genetics》2009,43(4):258-261
The additive-dominant system of genetic control of the protein content in the grain of winter wheat was established. A leading role of additive effects in the expression of the genes controlling the protein content was demonstrated. The intermediate type of inheritance of the grain’s level of protein content in hybrids was shown. 相似文献
16.
LIN WANG FA CUI JINPING WANG LI JUN ANMING DING CHUNHUA ZHAO XINGFENG LI DESHUN FENG JURONG GAO HONGGANG WANG 《Journal of genetics》2012,91(3):303-312
Grain protein content in wheat (Triticum aestivum L.) is generally considered a highly heritable character that is negatively correlated with grain yield and yield-related traits. Quantitative trait loci (QTL) for protein content was mapped using data on protein content and protein content conditioned on the putatively interrelated traits to evaluate possible genetic interrelationships between protein content and yield, as well as yield-related traits. Phenotypic data were evaluated in a recombinant inbred line population with 302 lines derived from a cross between the Chinese cultivar Weimai 8 and Luohan 2. Inclusive composite interval mapping using IciMapping 3.0 was employed for mapping unconditional and conditional QTL with additives. A strong genetic relationship was found between protein content and grain yield, and yield-related traits. Unconditional QTL mapping analysis detected seven additive QTL for protein content, with additive effects ranging in absolute size from 0.1898% to 0.3407% protein content, jointly accounting for 43.45% of the trait variance. Conditional QTL mapping analysis indicated two QTL independent from yield, which can be used in marker-assisted selection for increasing yield without affecting grain protein content. Three additional QTL with minor effects were identified in the conditional mapping. Of the three QTLs, two were identified when protein content was conditioned on yield, which had pleiotropic effects on those two traits. Conditional QTL mapping can be used to dissect the genetic interrelationship between two traits at the individual QTL level for closely correlated traits. Further, conditional QTL mapping can reveal additional QTL with minor effects that are undetectable in unconditional mapping. 相似文献
17.
内蒙古紫皮小麦紫皮性状的遗传分析 总被引:7,自引:0,他引:7
本文对内蒙古紫皮小麦进行了遗传分析。研究了这一材料的来源,普通小麦与内蒙古紫皮小麦杂交后代的细胞学特征,回交一代和杂种第二代的紫皮性状表现,以及内蒙古紫皮小麦、普通小麦和赖草的同工酶。综合研究的结果初步表明,这一紫皮小麦与国外报道的紫皮小麦材料不同。本材料是由普通小麦(Triticum aestivum L.)与赖草(Leymus dasystachys(Trin.) Dilger)杂交,经回交、自交后得到的,而国外报道的紫皮小麦材料是由普通六倍体小麦与四倍体紫皮小麦杂交得到的。内蒙古紫皮小麦很可能是一个异代换系,具有一对来自赖草的染色体,控制紫皮性状的基因在这对外源染色体上,其紫皮性状能稳定遗传,受控于一对显性基因。 相似文献
18.
Developmental Differences in Embryos of High and Low Protein Wheat Seeds during Germination
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Developmental patterns of embryos from high and low protein wheat (Triticum aestivum) grain produced under varied fertilizer conditions were compared. High protein grain produced seedlings 25% heavier with 25% more total RNA, 30% more DNA, 40% more amino acids, 60% more ribosomes, and 80% more soluble protein content than that of low protein seed. Consistently higher glutamine synthetase and α-amylase and lower acid phosphatase activities were observed in high protein seeds, though the isozyme pattern of α-amylase was not different in the two kinds of seeds. The high total ribosomes and particularly, polysome content observed in high protein seeds may be responsible for the rapid growth and high yield of these seeds. 相似文献
19.
Wheat provides a unique genetic system in which variable sink size is available across the ploidies. We characterized monocarpic senescence in diploid, tetraploid, and hexaploid wheat species in flag leaf from anthesis up to full grain maturity at regular intervals. Triticum tauschii Acc. cv. EC-331751 showed the fastest rate of senescence among the species studied and the rate of loss per day was highest in terms of photosynthesis rate, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) content, and flag leaf N content coupled with a higher rate of gain in grain N content. Cultivars Kundan and HD 4530 maintained high flag leaf N content throughout grain filling as compared to the diploids and showed a slower rate of senescence. RuBPCO content was higher in the diploids as compared to Kundan and HD 4530 at anthesis. However, the rate of decline in RuBPCO content per day was also higher in the diploids. This degradation in RuBPCO was mediated by high endoproteolytic activities in the diploids which in turn supported its higher rate of N mobilization as compared to the tetraploid and hexaploid wheat. Acidic endopeptidases were responsible for the mobilization of flag leaf nitrogen in wheat across ploidy levels (r=–0.582, p<0.01). 相似文献
20.
硫氮配施对持绿型小麦氮素运转及叶片衰老的影响 总被引:4,自引:0,他引:4
以持绿型小麦品种‘豫麦66号’、‘潍麦8号’及非持绿型品种‘小偃6号’为材料,采用以氮肥为主区硫肥为副区的田间裂区试验,研究了2个施氮水平[纯氮120kg/hm2(N120)、220kg/hm2(N220)]和3个施硫水平[纯硫0kg/hm2(S0)、20kg/hm2(S20)、60kg/hm2(S60)]下植株各部位的含氮量、叶片干鲜重及叶片叶绿素含量的变化,探讨硫氮配施对不同类型小麦氮吸收及衰老的影响。结果表明:在相同处理下,持绿型小麦植株的总含氮量、氮素转运量、叶绿素含量、叶片含水量及穗粒数和千粒重均高于非持绿型小麦。N120处理条件下,不同硫肥处理时持绿型小麦与非持绿型小麦变化趋势相同,开花期茎和叶含氮量及叶绿素含量在S60处理下均低于其他2个硫肥处理,生育后期叶片含水量下降幅度也明显高于其他处理;在N220处理条件下,3个品种开花期叶含氮量、收获期总氮累积量、氮收获指数、叶绿素含量及叶片含水量在S60处理下均高于其他2个处理,其中非持绿型小麦在高硫处理条件下灌浆期的叶绿素含量的增长率明显高于持绿型小麦,而灌浆中后期叶片含水量的下降幅度则明显低于持绿型小麦。研究发现,施用硫肥在氮肥不足时会对小麦植株氮素的吸收利用及叶片衰老等方面产生负面影响,但在氮肥充足时却在氮素的吸收利用、延缓叶片衰老及最终籽粒产量和总生物量等方面表现出正面效应;本实验条件下,220kg/hm2左右施氮量和60kg/hm2左右施硫量有利于各品种小麦生长发育和产量提高;高N和高S水平对于延缓小麦的衰老而言,非持绿性小麦比持绿型小麦更明显。 相似文献